Significance of Expression of TGF- in Pulmonary Metastasis in Non-small Cell Lung Cancer Tissues

Size: px
Start display at page:

Download "Significance of Expression of TGF- in Pulmonary Metastasis in Non-small Cell Lung Cancer Tissues"

Transcription

1 Originl Article Significnce of Expression of in Pulmonry Metstsis in Non-smll Cell Lung Cncer Tissues Hisshi Sji, Hruhiko Nkmur, Idiris Awut, Norihito Kwski, Msru Hgiwr, Akihiko Ogt, Mkoto Hosk, Tkmoto Sijo, Ysubumi Kto, nd Hrubumi Kto Recent studies hve evluted the cytokine network involved in the locl immune response to tumors. In ddition to infiltrting inflmmtory cells, tumors lso produce cytokines nd growth fctors tht my lter tumor growth nd tumor immunogenicity. Ninety-one smples of NSCLC were used in this study. We mesured the expression of, TNF-,,,,, INF-, nd in NSCLC tissues, by ELISA. The expression of nd were significntly higher in squmous cell crcinom thn in denocrcinom (p=0.016 nd p<0.001, respectively). The expression of, nd were significntly higher in pulmonry metstsis positive thn negtive cses (p=0.002, p=0.001, nd p=0.008, respectively). In multivrite logistic regression nlysis, the expression of ws n independent risk fctor for the occurrence of pulmonry metstsis (p=0.008, 95% CI= ). We confirmed tht tumor infiltrting stroml cells were mjor sources of by immunohistochemicl nlysis. The expression of nd were significntly higher in cses with centrl necrosis (p=0.006 nd p=0.011, respectively). We speculted tht expression in tumor infiltrting stroml cells my regulte the occurrence of spontneous pulmonry metstsis in NSCLC. (Ann Thorc Crdiovsc Surg 2003; 9: ) Key words: cytokine,, pulmonry metstsis, non-smll cell lung cncer Introduction From Pulmonry Oncology Unit, Deprtment of Surgery, Tokyo Medicl University, Tokyo, Jpn Received My 22, 2003; ccepted for publiction August 7, Address reprint requests to Hisshi Sji: Deprtment of Surgery, Tokyo Medicl University, 6-7-1, Nishishinjuku, Shinjuku-ku, Tokyo , Jpn. Recent studies hve evluted the cytokine network involved in the locl immune response to tumors, 1-4) In ddition to infiltrting inflmmtory cells, tumors lso produce cytokines nd growth fctors tht my lter tumor growth, tumor immunogenicity, nd the host immune response. A vriety of tumor-derived fctors, including cytokines such s interleukin (IL)-4, 5), 6), 7) IL- 10, 5) trnsforming growth fctor (TGF)-, 8) vsculr endothelil growth fctor (), 9-11) nd monocyte chemottrctnt protein (MCP) ) my either regulte tumor growth or lter the nti-tumor immune response. Thus, humn cncer growth my be regulted by vriety of cytokines vi both utocrine nd prcrine pthwys. Regultion of tumor growth by cytokines my occur directly by regultion of cell prolifertion or indirectly through effects on ngiogenesis or host immunity. Cytokine responses hve been brodly ctegorized into two min types. T helper 1 (Th1) cytokines, such s IL-2,, interferon (IFN)-, nd tumor necrosis fctor (TNF)- re responsible for cell-medited immunity. Th2 type cytokines, such s IL-4, IL-5, IL- 6, IL-10, nd IL-13, re cpble of stimulting humorl immunity. Recent studies hve indicted Th2 cytokine pttern t the tumor site nd suggested tht these cytokines my medite immunosuppression. 3,5,13) In this study, we ssessed the expression of severl cytokines, such s,, nd IFN-, chemokines, such s nd, nd ngiogenic fctors, such s, TNF-, nd in tumor tissues nd evluted the correltion with clinicopthologicl fctors in humn non-smll cell lung cncer (NSCLC). 295

2 Sji et l. Mterils nd Methods Lung cncer tissues Tissues from 91 rndomly selected primry NSCLC cses, resected surgiclly t Tokyo Medicl University from My 2000 to July 2001, were used in this investigtion. Representtive prts of the specimens were frozen in liquid nitrogen immeditely fter surgicl resection nd stored t 80 C until preprtion of tissue extrcts. The min chrcteristics of the ptients re described in Tble 1. Informed consent ws obtined from ll ptients. Smple preprtion for ELISA Tumor tissues were homogenized in 10 volumes of 10 mm Tris-HCl buffer (ph 7.4) contining 15 mm NCl, 1.5 mm MgCl2, nd 50 M potssium phosphte nd then centrifuged t 10,000 g for 15 min. The superntnt ws stored t 80 C until use. A portion of the superntnt ws dilyzed overnight t 4 C in buffer contining 20 mm potssium phosphte (ph 7.4) nd 1 mm 2- mercptoethnol nd then used for enzyme-linked immunosorbent ssy (ELISA). Mesurement of cytokines, chemokines, nd ngiogenic fctors The concentrtions of Th1 cytokines ( nd INF- ), Th2 cytokines (), chemokines ( nd ), nd ngiogenic fctors (, TNF-, nd ) in the tumor extrcts were mesured by ELISA (BioSource Interntionl, Cmrillo, CA). The mesurements were performed ccording to the mnufcturer s instructions. Absorbnce of ech smple ws determined with n ELISA plte reder (Model 450 Microplte Reder, Bio-Rd Lbortories). The miniml detection limits for the vrious fctors were s follows: +p40, 1.0 pg/ml; IFN-, 4.0 pg/ml;, 2.0 pg/ml;, 5.0 pg/ml;, 20.0 pg/ml;, 5.0 pg/ml; TNF-, 1.7 pg/ml; nd, 2.0 pg/ml. The totl protein concentrtion of the superntnts extrcted from tumor tissue ws determined with DC protein ssy kit (Bio-Rd Lbortories). The results re expressed s pg of cytokine/ mg of totl protein. Immunohistochemicl nlysis of The 3-5 m sections of prffin-embedded tumor tissues were pplied to indirect nti-peroxidse immunohistochemicl ssy, Strep ABC technique (Dko, Crpentri, CA) for ssessing the expression of. Anlysis of ws crried out using nti-humn Tble 1. Ptient chrcteristics Enrolled ptients Age (men nd rnge) Sex M/F Pthologicl type Adenocrcinom Squmous cell crcinom Lrge cell crcinom p-stge I/II/III/IV Tumor size (men nd rnge, cm) p-t fctor T1, T2/T3, T4 p-n fctor N0/N1/N2/N3 p fctor p0/p1/p2/p3 pm fctor pm0/pm1 Centrl necrosis grde 0/1/ (33-84) 57/34 64 (70%) 19 (21%) 8 (9%) 54/13/20/2 3.3 ( ) 73/18 68/14/8/1 63/12/7/9 82/9 47/23/19 p, pthologicl; pm1, intrpulmonry metstsis in only sme lobe; pm0, intrpulmonry metstsis negtive. ntibody (Genzyme/Techne, Cmbridge, MA) t 1:100 dilutions. On ech section five microscopic fields, which hd the most ccumulted positive signls (hot spots) were selected. The percentge of stined cells in these res ws counted, nd men vlue of these percentges ws used s n expression vlue of. For further nlysis, vlues of ech smple were ctegorized into two groups (negtive, 0-30%; positive, >30%). Immunohistochemicl nd pthologicl ssessments were conducted by two investigtors, completely blinded to ny clinicl informtion. Pthologicl sttus Centrl necrosis grde ws clssified by H&E stining s follows: negtive, no centrl necrosis; positive, moderte to mrked centrl necrosis. Intrpulmonry metstsis (pm) ws clssified ccording to Clssifiction of Lung Cncer of the Jpn Lung Cncer Society: pm0, no intrpulmonry metstsis; pm1, metstsis to the sme lobe s tht of the primry tumor. 14) Sttisticl nlysis Unpired groups were compred by Student s t-test. For qulittive vribles, Person chi-squre coefficient ws clculted. A p vlue of less thn 0.05 ws tken to indicte sttisticlly significnt difference. Multivrite logistic regression nlysis ws performed to evlute possible ssocitions between different vribles nd the occurrence of pm1. Only vribles significnt by univrite nlysis were included in the logistic regression model. All sttisticl nlyses were crried out using SttView 5.0 softwre (SAS Institute Inc.). 296

3 Significnce of Expression of in Pulmonry Metstsis in Non-smll Cell Lung Cncer Tissues Tble 2. Expression of cytokines, chemokines, nd ngiogenic fctors in 91 NSCLC tissues Biologicl mrkers TNF- IFN- Results Medin (rnge) pg/mg ( ) pg/mg ( ) pg/mg ( ) pg/mg ( ) pg/mg ( ) pg/mg ( ) pg/mg ( ) pg/mg ( ), vsculr endothelil growth fctor; TNF-, tumor necrosis fctor- ;, trnsforming growth fctor- ; IL, interleukin; IFN-, interferon- ;, monocyte chemottrctnt protein-1. Expression of cytokines, chemokines, nd ngiogenic fctors, TNF-,,,,, nd were vriously secreted in lung cncer tissues, which were mesured by ELISA. There were only three detectble cses of TNF- in 91 cses. No cse hd detectble levels of IFN- in this series. These fctors were therefore excluded from the nlysis. The medin vlues nd the rnge of ech biologicl mrker re shown in Tble 2. Correltion between biologicl mrkers nd pthologicl type The expression of nd were significntly higher in squmous cell crcinom thn in denocrcinom (p= nd p<0.001, respectively) (Tble 3). Correltion between biologicl mrkers, clinicopthologicl fctor, nd pm fctor Pulmonry metstsis in only one lobe: pm1 ws detected in 9 of 91 cses (9.9%). The size of pulmonry metstsis nodules rnged from 0.3 to 1.0 cm. On the other hnd, the size of primry lesions rnged from 2.0 to 13.0 cm. Among clinicopthologicl fctors, tumor size nd pthologicl nodule involvement significntly correlted with pm1 (p= nd p=0.0296, respectively). Among biologicl mrkers, the expression of,, nd MCP- 1 were significntly higher in primry lung cncer tissues of cses with pm1 (p=0.0017, p=0.0075, nd , respectively) (Tble 4). Moreover, in multivrite logistic regression nlysis, the expression of ws n independent risk fctor of the occurrence of pm1 (Tble 5). Correltion between biologicl mrkers nd centrl necrosis Expression of nd were significntly higher in centrl necrosis cses thn in cses without centrl necrosis (p=0.006 nd p=0.011, respectively) (Tble 6). Expression of NSCLC tissues by immunohistochemicl nlysis We evluted expression in rndomly selected 36 NSCLC tissues by immunohistochemicl nlysis. TGF- expression in tumor cells ws observed in 21 out of 36 cses (58%) (Fig. 1). Twenty-seven out of 36 cses (75%) showed expression in stroml cells, especilly tumor-infiltrting inflmmtory cells in tumor strom (Fig. 1b). High expression of in tumor cells nd stroml cells ws found in 9 (25%) nd 13 (36%) of cses, respectively. Discussion Humn cncer growth my be regulted by vriety of cytokines vi both utocrine nd prcrine pthwys. Regultion of tumor growth by cytokines my occur directly by regultion of cell prolifertion or indirectly through effects on ngiogenesis or host immunity. In this study, we ssessed the expression of severl meditors, including immune cytokines, such s,, nd IFN-, chemokines, such s nd, nd ngiogenic fctors, such s, TNF-, nd by the ELISA technique in 91 smples of NSCLC tissues to clrify the role of the cytokine network in the tumor tissues for the development of humn NSCLC. Severl studies reported expression of vrious cytokines in NCSLC tissues by using immunohistochemicl stins or RT-PCR or ELISA method. 3,13) In this study, the expression of,, nd ws high, while the expression of,, nd ws moderte in NCLC tissues. However, the expression of TNF-, which is one of the mjor nti-tumor cytokines, ws mesurble in only three cses, nd tht of IFN-, which is lso one of the mjor nti-tumor cytokines, ws not detectble in this series (Tble 2). Anti-tumor cytokines, such s TNF- nd IFN- were few secreted in tumor tissues, compred with in serum in NSCLC, which previous study reported. 15) With regrd to the correltion with clinicopthologicl vribles, we found three significnt correltions in the present study. First, the expression of nd were significntly higher in squmous cell crcinom thn 297

4 Sji et l. Tble 3. Correltion between biologicl mrkers nd pthologicl type Adenocrcinom (n=64) men±sd (pg/mg) Squmous cell crcinom (n=19) men±sd (pg/mg) p vlue ± ± ± ± ± ± ± ± ± ± ± ± < Sttisticl nlysis clculted ccording to Student s t-test. : Sttisticlly significnt, vsculr endothelil growth fctor; TNF-, tumor necrosis fctor- ;, trnsforming growth fctor- ; IL, interleukin;, monocyte chemottrctnt protein-1. Tble 4. Correltion between biologicl meditors, clinicopthologicl fctor nd pm fctor pm1 (n=9) men±sd (pg/mg) pm0 (n=82) men±sd (pg/mg) p vlue Tumor size (cm) p-n sttus (+/ ) 4.889± / ± ± ± ± ± ± ± / ± ± ± ± ± ± b b b b b : pm1 nodule size = cm b : Sttisticlly significnt, vsculr endothelil growth fctor;, trnsforming growth fctor- ; IL, interleukin;, monocyte chemottrctnt protein-1. Tble 5. Reltionship between occurrence of pm1 nd clinicl fctors or cytokine expression by multivrite logistic regression nlysis Clinicl fctors nd cytokines p vlue Odds rtio 95% CI Tumor size (cm) p-n sttus (+/ ) : Sttisticlly significnt, trnsforming growth fctor- ; IL, interleukin;, monocyte chemottrctnt protein-1. in denocrcinom in NSCLC tissues, which hs not previously been reported. is one of the inflmmtory cytokines, which my contribute to n incresed expression of MHC molecules, while is one of the mjor CXC chemokines cpble of inducing chemotctic migrtion of neutrophils. It is importnt to note tht these specific findings my be reflected in the different clinicl behviors nd immune response of squmous cell nd denocrcinoms. The second result ws tht the expression of,, nd positively correlted with the occurrence of pulmonry metstsis, pm1, in ddition to clinicopthologicl fctors, such s tumor size nd p-n sttus, by univrite nlysis (Tble 4). Moreover, multivri- 298

5 Significnce of Expression of in Pulmonry Metstsis in Non-smll Cell Lung Cncer Tissues Tble 6. Correltion between biologicl meditors nd centrl necrosis Centrl necrosis [men±sd (pg/mg)] + p vlue ± ± ± ± ± ± ± ± ± ± ± ± Sttisticl nlysis were clculted ccording to Student s t-test. : Sttisticlly significnt, vsculr endothelil growth fctor; TNF-, tumor necrosis fctor- ; TGF-, trnsforming growth fctor- ; IL, interleukin;, monocyte chemottrctnt protein-1. b Fig. 1. Immunohistochemicl nlysis of in NSCLC tissues. Positive stining of ws seen in tumor cells () nd in tumor infiltrting stroml cells (b). te logistic regression nlysis reveled tht the expression of remined s n independent risk fctor of the occurrence of pm1 (Tble 5). We confirmed tht both tumor cells nd tumor infiltrting stroml cells were mjor sources of by immunohistochemicl nlysis (Fig. 1). Furthermore, the expression sttus of in stroml cells by immunohistochemicl stin significntly positively correlted with the vlue of in tumor tissues by ELISA (p=0.0279), s compred with immunohistochemicl expression of in tumor cells (p=0.8282), using Student s t-test nlysis. hs been reported to be multifunctionl growth fctor, synthesized by wide vriety of both norml nd mlignnt cells. The effect of on tumor growth is controversil, nd it is ble to directly inhibit prolifertion of epithelil cncer cells, but its immunosuppressive properties cn lso promote tumor growth. In this study, we suggested tht expression of in the tumor microenvironment promotes the development of spontneous pulmonry metstsis by its immunosuppressive properties in NSCLC. Lst, we showed tht nd expression hve significnt reltionship with the sttus of centrl necrosis (Tble 6). Some studies hve reported tht hypoxi in tumor tissues, such s centrl necrosis, induced tumor ngiogenesis in brest cncer nd NSCLC. 16,17) Hypoxi incresed the expression nd secretion of nd IL- 8 in vitro. 18) The hypoxi-induced neovsculriztion ws found to be medited by, multifunctionl cytokine tht shows potent ngiogenic ctivities in vitro nd in vivo, nd possibly by, strong specific mitogen for endothelil cells ) We ssumed tht our result supports this hypothesis. In ddition, recent studies reported tht serum levels of proinflmmtory cytokines, such s IL-1,, nd TNF-, 15) nti-tumor cytokines, such s IL-2 nd IL-10, 22) 299

6 Sji et l. nd chemokines, such s 23) hve prognostic significnce for survivl of NSCLC ptients. We could not detect ny fctors tht correlted with the sttus of nodl involvement, which is known to be the worst prognostic fctor in NSCLC in this series (dt is not shown). We did not ssess the prognostic significnce of these cytokines, becuse of short medin follow-up terms such s 2.1 yers to less thn 3 yers. Cytokine expression in tumor microenvironments is thought to be importnt in the medition of both host immune response nd tumor survivl. The source of these cytokines includes tumor cells, infiltrting leukocytes, fibroblsts, nd other stroml elements. Therefore, understnding the bsis of moleculr nd cellulr cross-tlk between tumor cells nd the immune system of the host in locl re remins crucil issue in the effective use of cytokines in the biotherpy of cncer. Acknowledgment The uthors re indebted to Prof. J. Ptrick Brron of the Interntionl Medicl Communictions Center of Tokyo Medicl University for his review of this mnuscript. References 1. North RJ. The immune response to tumors. Trnsplnt Proc 1989; 21: Prehn RT. Tumor immunogenicity: how fr cn it be pushed? Proc Ntl Acd Sci U S A 1993; 90: Ymmur M. Modlin RL, Ohmen JD, et l. Locl expression of nti-inflmmtory cytokines in cncer. J Clin Invest 1993; 91: Allev DG, Burger CJ, Elgert KD. Tumor-induces regultion of suppressor mcrophge nitric oxide nd TNF- production: role of tumor-derived IL-10, nd prostglndin E2. J Immunol 1994; 153: Hung M, Wng J, Lee P, et l. Humn non-smll cell lung cncer cells express type 2 cytokine pttern. Cncer Res 1995; 55: Tkizw H. Ohtoshi T, Oht K, et l. Growth inhibition of humn lung cncer cell lines by interleukin 6 in vitro: possible role in tumor growth vi n utocrine mechnism. Cncer Res 1993; 53: Smith DR, Polverini PJ, Kunkel SL, et l. Inhibition of interleukin-8 ttenutes ngiogenesis in bronchogenic crcinom. J Exp Med 1994; 197: Siegfried JM, Owens SE. Response of primry humn lung crcinoms to utocrine growth fctors produced by lung crcinom cell line. Cncer Res 1988; 48: Yun A, Yu CJ, Chen WJ, et l. Correltion of totl mrna nd protein expression with histologic type, tumor ngiogenesis, ptient survivl nd timing of relpse in non-smll-cell lung cncer. Int J Cncer 2000; 89: Ueno T, Toi M, Sji H, et l. Significnce of mcrophge chemottrctnt protein-1 in mcrophge recruitment, ngiogenesis, nd survivl in humn brest cncer. Clin Cncer Res 2000; 6: Toi M, Gion M, Sji H, et l. Endogenous interleukin- 12: reltionship with ngiogenic fctors, hormone receptors nd nodl sttus in humn brest crcinom. Int J Oncol 1999; 15: Sji H, Koike M, Ymori T, et l. Significnt correltion of monocyte chemottrctnt protein-1 expression with neovsculriztion nd progression of brest crcinom. Cncer 2001; 92: Boldrini L, Clcini A, Smritni E, et l. Tumour necrosis fctor-lph nd trnsforming growth fctorbet re significntly ssocited with better prognosis in non-smll cell lung crcinom: puttive reltion with BCL-2-medited neovsculriztion. Br J Cncer 2000; 83: The Jpn Lung Cncer Society. Clssifiction of Lung Cncer. Tokyo: Knehr & Co., 2000; p De Vit F, Orditur M, Auriemm A, et l. Serum concentrtions of proinflmmtory cytokines in dvnced non smll cell lung cncer ptients. J Exp Clin Cncer Res 1998; 17: Leek RD, Lnders RJ, Hrris AL, et l. Necrosis correltes with high vsculr density nd focl mcrophge infiltrtion in invsive crcinom of the brest. Br J Cncer 1999; 79: Gitromnolki A, Koukourkis MI, Sivridis E, et l. Expression of hypoxi-inducible crbonic nhydrse- 9 reltes to ngiogenic pthwys nd independently to poor outcome in non-smll cell lung cncer. Cncer Res 2001; 61: Rofstd EK, Dnielsen T. Hypoxi-induced metstsis of humn melnom cells: involvement of vsculr endothelil growth fctor-medited ngiogenesis. Br J Cncer 1999; 80: Br-Eli M. Role of interleukin-8 in tumor growth nd metstsis of humn melnom. Pthobiology 1999; 67: Ferrr N, Dvis-Smyth T. The biology of vsculr endothelil growth fctor. Endocr Rev 1997; 18: Rofstd EK, Hlsor EF. Hypoxi-ssocited spontneous pulmonry metstsis in humn melnom xenogrfts: involvement of microvsculr hot spots induced in hypoxic foci by interleukin 8. Br J Cncer 2002; 86: Neuner A, Schindel M, Wildenberg U, et l. Prognostic significnce of cytokine modultion in non-smll cell lung cncer. Int J Cncer 2002; 20: Orditur M, De Vit F, Ctlno G, et l. Elevted serum levels of interleukin-8 in dvnced non-smll cell lung cncer ptients: reltionship with prognosis. J Interferon Cytokine Res 2002; 22:

Esophageal carcinoma is the eighth most common cancer

Esophageal carcinoma is the eighth most common cancer ORIGINAL ARTICLE Tumor-Strom Rtio Is n Independent Predictor for Survivl in Esophgel Squmous Cell Crcinom Ki Wng, MD,* Wei M, MD,* Jinbo Wng, MD,* Ling Yu, MD, Xiomei Zhng, MD, Zhenbo Wng, MD, Bingxu Tn,

More information

CheckMate 153: Randomized Results of Continuous vs 1-Year Fixed-Duration Nivolumab in Patients With Advanced Non-Small Cell Lung Cancer

CheckMate 153: Randomized Results of Continuous vs 1-Year Fixed-Duration Nivolumab in Patients With Advanced Non-Small Cell Lung Cancer CheckMte 53: Rndomized Results of Continuous vs -Yer Fixed-Durtion Nivolumb in Ptients With Advnced Non-Smll Cell Lung Cncer Abstrct 297O Spigel DR, McCleod M, Hussein MA, Wterhouse DM, Einhorn L, Horn

More information

Lung cancer is the leading cause of cancer death worldwide, EGFR Mutation and Brain Metastasis in Pulmonary Adenocarcinomas

Lung cancer is the leading cause of cancer death worldwide, EGFR Mutation and Brain Metastasis in Pulmonary Adenocarcinomas Originl Article EGFR Muttion nd Brin Metstsis in Pulmonry Adenocrcinoms Dong-Yeop Shin, MD,* Im Il N, MD,* Cheol Hyeon Kim, MD, PhD, Sunhoo Prk, MD, PhD, HeeJong Bek, MD, PhD, nd Sung Hyun Yng, MD, PhD*

More information

Impact of Positive Nodal Metastases in Patients with Thymic Carcinoma and Thymic Neuroendocrine Tumors

Impact of Positive Nodal Metastases in Patients with Thymic Carcinoma and Thymic Neuroendocrine Tumors Originl Article Impct of Positive Nodl Metstses in Ptients with Thymic Crcinom nd Thymic Neuroendocrine Tumors Benny Weksler, MD, Anthony Holden, MD, nd Jennifer L. Sullivn, MD Introduction: Thymic crcinoms

More information

Prognostic significance of pretreatment serum levels of albumin, LDH and total bilirubin in patients with nonmetastatic

Prognostic significance of pretreatment serum levels of albumin, LDH and total bilirubin in patients with nonmetastatic Crcinogenesis, 2015, Vol. 36, No. 2, 243 248 doi:10.1093/crcin/bgu247 Advnce Access publiction December 18, 2014 Originl Mnuscript originl mnuscript Prognostic significnce of pretretment serum levels of

More information

ORIGINAL ARTICLE ABSTRACT INTRODUCTION

ORIGINAL ARTICLE ABSTRACT INTRODUCTION ORIGINAL ARTICLE LOSS OF EPCAM STAINING CORRELATES WITH POOR OUTCOME IN CRC, Wng et l. Reduction in membrnous immunohistochemicl stining for the intrcellulr domin of epithelil cell dhesion molecule correltes

More information

One of the most important biological mechanisms of

One of the most important biological mechanisms of Brief Report Serum Thymidine Kinse 1 Activity in the Prognosis nd Monitoring of Chemotherpy in Lung Cncer Ptients: A Brief Report Benjmin Nismn, PhD,* Hovv Nechushtn, MD, PhD,* Him Birn, MD, Hds Gntz-Sorotsky,

More information

Supplementary Figure 1

Supplementary Figure 1 doi: 1.138/nture6188 SUPPLEMENTARY INFORMATION Supplementry Figure 1 c CFU-F colonies per 1 5 stroml cells 14 12 1 8 6 4 2 Mtrigel plug Neg. MCF7/Rs MDA-MB-231 * * MCF7/Rs-Lung MDA-MB-231-Lung MCF7/Rs-Kidney

More information

Association of PTEN expression with liver function and inflammatory changes in patients with liver cancer after chemotherapy

Association of PTEN expression with liver function and inflammatory changes in patients with liver cancer after chemotherapy ONCOLOGY LETTERS Assocition of PTEN expression with liver function nd inflmmtory chnges in ptients with liver cncer fter chemotherpy JIXIANG ZHOU nd XIAOLI LI Deprtment of Heptobiliry Surgery, Xingy Hospitl,

More information

ONCOLOGY LETTERS 14: , China Medical University, Shenyang, Liaoning , P.R. China

ONCOLOGY LETTERS 14: , China Medical University, Shenyang, Liaoning , P.R. China 4890 Expression of vsculr endothelil growth fctor nd cspse 3 in mucinous brest crcinom nd infiltrting ductl crcinom not otherwise specified, nd the correltion with disese free survivl QIULI WANG 1, LISHA

More information

Journal of Hainan Medical University.

Journal of Hainan Medical University. 132 Journl of Hinn Medicl University 2017; 23(11): 132-136 Journl of Hinn Medicl University http://www.hnykdxxb.com Assessment of the efficcy nd sfety of bronchil rtery perfusion chemotherpy combined with

More information

Original Article Prognostic and clinicopathologic significance of AEG-1/MTDH and E-cadherin expression in human gallbladder carcinoma

Original Article Prognostic and clinicopathologic significance of AEG-1/MTDH and E-cadherin expression in human gallbladder carcinoma Int J Clin Exp Pthol 2018;11(12):6025-6031 www.ijcep.com /ISSN:1936-2625/IJCEP0086349 Originl Article Prognostic nd clinicopthologic significnce of AEG-1/MTDH nd E-cdherin expression in humn gllbldder

More information

Aberrant expression of B7-H4 correlates with poor prognosis and suppresses tumor-infiltration of CD8 + T lymphocytes in human cholangiocarcinoma

Aberrant expression of B7-H4 correlates with poor prognosis and suppresses tumor-infiltration of CD8 + T lymphocytes in human cholangiocarcinoma ONCOLOGY REPORTS 36: 419-427, 2016 Aberrnt expression of B7-H4 correltes with poor prognosis nd suppresses tumor-infiltrtion of CD8 + T lymphocytes in humn cholngiocrcinom Xin Zho *, Fei Guo *, Zhonghu

More information

Implications for Human Leukocyte Antigen Antibodies After Lung Transplantation. A 10-Year Experience in 441 Patients

Implications for Human Leukocyte Antigen Antibodies After Lung Transplantation. A 10-Year Experience in 441 Patients CHEST Originl Reserch TRANSPLANTATION Implictions for Humn Leukocyte Antigen Antibodies After Lung Trnsplnttion A 10-Yer Experience in 441 Ptients Lurie D. Snyder, MD, MHS ; Ziwei Wng, BS ; Dong-Feng Chen,

More information

Assessment of Depression in Multiple Sclerosis. Validity of Including Somatic Items on the Beck Depression Inventory II

Assessment of Depression in Multiple Sclerosis. Validity of Including Somatic Items on the Beck Depression Inventory II Assessment of Depression in Multiple Sclerosis Vlidity of Including Somtic Items on the Beck Depression Inventory II Peggy Crwford, PhD; Noh J. Webster, MA Signs nd symptoms of multiple sclerosis (MS)

More information

Efficacy of Pembrolizumab in Patients With Advanced Melanoma With Stable Brain Metastases at Baseline: A Pooled Retrospective Analysis

Efficacy of Pembrolizumab in Patients With Advanced Melanoma With Stable Brain Metastases at Baseline: A Pooled Retrospective Analysis Efficcy of Pembrolizumb in Ptients With Advnced Melnom With Stble Brin Metstses t Bseline: A Pooled Retrospective Anlysis Abstrct 1248PD Hmid O, Ribs A, Dud A, Butler MO, Crlino MS, Hwu WJ, Long GV, Ancell

More information

Clinical manifestations in patients with alpha-fetoprotein producing gastric cancer

Clinical manifestations in patients with alpha-fetoprotein producing gastric cancer Curr Oncol, Vol. 21, pp. e394-399; doi: http://dx.doi.org/10.3747/co.21.1768 CLINICAL MANIFESTATIONS IN AFP PRODUCING GASTRIC CANCER ORIGINAL ARTICLE Clinicl mnifesttions in ptients with lph-fetoprotein

More information

CHEST. Thyroid transcription factor 1 (TTF-1) is an important. Original Research

CHEST. Thyroid transcription factor 1 (TTF-1) is an important. Original Research CHEST Originl Reserch Clinicl Significnce of Thyroid Trnscription Fctor-1 in Advnced Lung Adenocrcinom Under Epiderml Growth Fctor Receptor Tyrosine Kinse Inhibitor Tretment Kuei-Pin Chung, MD; Yen-Tsung

More information

Effects of TRPM8 on the proliferation and angiogenesis of prostate cancer PC-3 cells in vivo

Effects of TRPM8 on the proliferation and angiogenesis of prostate cancer PC-3 cells in vivo ONCOLOGY LETTERS 2: 1213-1217, 2011 Effects of TRPM8 on the prolifertion nd ngiogenesis of prostte cncer PC-3 cells in vivo GUANGBIN ZHU, XINGHUAN WANG, ZHONGHUA YANG, HONG CAO, ZHE MENG, YONGZHI WANG

More information

American Joint Committee on Cancer Staging and Clinicopathological High-Risk Predictors of Ocular Surface Squamous Neoplasia

American Joint Committee on Cancer Staging and Clinicopathological High-Risk Predictors of Ocular Surface Squamous Neoplasia Americn Joint Committee on Cncer Stging nd Clinicopthologicl High-Risk Predictors of Oculr Surfce Squmous Neoplsi A Study From Tertiry Eye Center in Indi Sheetl Chuhn, MSc; Seem Sen, MD; Anjn Shrm, PhD;

More information

Prognostic factors in tongue cancer relative importance of demographic, clinical and histopathological factors

Prognostic factors in tongue cancer relative importance of demographic, clinical and histopathological factors British Journl of Cncer (2000) 83(5), 614 619 doi: 10.1054/ bjoc.2000.1323, vilble online t http://www.idelibrry.com on Prognostic fctors in tongue cncer reltive importnce of demogrphic, clinicl nd histopthologicl

More information

Extraction and Some Functional Properties of Protein Extract from Rice Bran

Extraction and Some Functional Properties of Protein Extract from Rice Bran Ksetsrt J. (Nt. Sci.) 40 : 209-214 (2006) Extrction nd Some Functionl Properties of Protein Extrct from Rice Brn Chockchi Theerkulkit*, Siree Chiseri nd Siriwt Mongkolknchnsiri ABSTRACT Rice brn protein

More information

Study on the association between PI3K/AKT/mTOR signaling pathway gene polymorphism and susceptibility to gastric

Study on the association between PI3K/AKT/mTOR signaling pathway gene polymorphism and susceptibility to gastric JBUON 2017; 22(6): 1488-1493 ISSN: 1107-0625, online ISSN: 2241-6293 www.jbuon.com E-mil: editoril_office@jbuon.com ORIGINAL ARTICLE Study on the ssocition between PI3K/AKT/mTOR signling pthwy gene polymorphism

More information

MICRO RNA-21 EXPRESSION LEVELS IN INVASIVE BREAST CARCINOMA WITH A NON-INVASIVE COMPONENT

MICRO RNA-21 EXPRESSION LEVELS IN INVASIVE BREAST CARCINOMA WITH A NON-INVASIVE COMPONENT Arch. Biol. Sci., Belgrde, 67(4), 1285-1295, 2015 DOI:10.2298/ABS150327105P MICRO RNA-21 EXPRESSION LEVELS IN INVASIVE BREAST CARCINOMA WITH A NON-INVASIVE COMPONENT Nin Petrović 1,*, Snežn Jovnović-Ćupić

More information

Body mass index, waist-to-hip ratio, and metabolic syndrome as predictors of middle-aged men's health

Body mass index, waist-to-hip ratio, and metabolic syndrome as predictors of middle-aged men's health Originl Article - Sexul Dysfunction/Infertility pissn 2005-6737 eissn 2005-6745 Body mss index, wist-to-hip rtio, nd metbolic syndrome s predictors of middle-ged men's helth Jung Hyun Prk *, In-Chng Cho

More information

Supplementary Online Content

Supplementary Online Content Supplementry Online Content Zulmn DM, Pl Chee C, Ezeji-Okoye SC, et l. Effect of n intensive outptient progrm to ugment primry cre for high-need Veterns Affirs ptients: rndomized clinicl tril. JAMA Intern

More information

Genetic polymorphisms in the TERT-CLPTM1L region and lung cancer susceptibility in Chinese males

Genetic polymorphisms in the TERT-CLPTM1L region and lung cancer susceptibility in Chinese males 1588 Genetic polymorphisms in the TERT-CLPTM1L region nd lung cncer susceptibility in Chinese mles XUYANG XIAO 1 nd WUBIN HE 2 1 Deprtment of Thorcic Surgery nd 2 Biologicl Therpy Center Lbortory, The

More information

Diabetes mellitus secondary to pancreatic diseases (type 3c): The effect of smoking on the exocrine endocrine interactions of the pancreas

Diabetes mellitus secondary to pancreatic diseases (type 3c): The effect of smoking on the exocrine endocrine interactions of the pancreas 764062DVR0010.1177/1479164118764062Dibetes & Vsculr Disese ReserchŚliwińsk-Mossoń et l. reserch-rticle2018 Originl Article Dibetes mellitus secondry to pncretic diseses (type 3c): The effect of smoking

More information

Invasive Pneumococcal Disease Quarterly Report. July September 2017

Invasive Pneumococcal Disease Quarterly Report. July September 2017 Invsive Pneumococcl Disese Qurterly Report July September 2017 Prepred s prt of Ministry of Helth contrct for scientific services by Rebekh Roos Helen Heffernn October 2017 Acknowledgements This report

More information

Possible predictive role of cancer/testis antigens in breast ductal carcinoma in situ

Possible predictive role of cancer/testis antigens in breast ductal carcinoma in situ ONCOLOGY LETTS Possible predictive role of cncer/testis ntigens in brest ductl crcinom in situ ANA ROGULJIC 1, GULIO SPAGNOLI 2, ANTONIO JURETIC 3, BOZENA SARCEVIC 4, MARIJA BANOVIC 5 nd LIDIJA BEKETIC

More information

High EGFR mrna expression is a prognostic factor for reduced survival in pancreatic cancer after gemcitabine-based adjuvant chemotherapy

High EGFR mrna expression is a prognostic factor for reduced survival in pancreatic cancer after gemcitabine-based adjuvant chemotherapy INTERNATIONAL JOURNAL OF ONCOLOGY 38: 629-641, 2011 High EGFR mrna expression is prognostic fctor for reduced survivl in pncretic cncer fter gemcitbine-bsed djuvnt chemotherpy Hyto Fujit 1, Kenoki Ohuchid

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Prentl doi:.8/nture57 Figure S HPMECs LM Cells Cell lines VEGF (ng/ml) Prentl 7. +/-. LM 7. +/-.99 LM 7. +/-.99 Fold COX induction 5 VEGF: - + + + Bevcizum: - - 5 (µg/ml) Reltive MMP LM mock COX MMP LM+

More information

Original Article Serum tumor markers used for predicting esophagogastric junction adenocarcinoma in esophageal malignancy

Original Article Serum tumor markers used for predicting esophagogastric junction adenocarcinoma in esophageal malignancy Int J Clin Exp Med 2016;9(6):11859-11864 www.ijcem.com /ISSN:1940-5901/IJCEM0025330 Originl Article Serum tumor mrkers used for predicting esophgogstric junction denocrcinom in esophgel mlignncy Yongkng

More information

Supplementary Figure 1

Supplementary Figure 1 Roles of endoplsmic reticulum stress-medited poptosis in -polrized mcrophges during mycocteril infections Supplementry informtion Yun-Ji Lim, Min-Hee Yi, Ji-Ae Choi, Jung-hwn Lee, Ji-Ye Hn, Sung-Hee Jo,

More information

Comparison of three simple methods for the

Comparison of three simple methods for the J. clin. Pth. (1967), 2, 5 Comprison of three simple methods for the ssessment of 'free' thyroid hormone T. M. D. GIMLETTE1 From the Rdio-Isotope Lbortory, St. Thoms's Hospitl, London SYNOPSIS A dilysis

More information

RESEARCH ARTICLE. Wen Li 1, Jing Deng 2 *, Shuang-Shuang Wang 1, Liang Ma 1, Jiang Pei 1, Xiao-Xi Zeng 1, Jian-Xin Tang 1. Abstract.

RESEARCH ARTICLE. Wen Li 1, Jing Deng 2 *, Shuang-Shuang Wang 1, Liang Ma 1, Jiang Pei 1, Xiao-Xi Zeng 1, Jian-Xin Tang 1. Abstract. DOI:http://dx.doi.org/10.7314/APJCP.2014.15.24.10937 Methyltion of the RAR-β Gene nd Cigrette Smoking in NSCLC in Southern-Centrl Chinese RESEARCH ARTICLE Assocition of Methyltion of the RAR-β Gene with

More information

Geographical influence on digit ratio (2D:4D): a case study of Andoni and Ikwerre ethnic groups in Niger delta, Nigeria.

Geographical influence on digit ratio (2D:4D): a case study of Andoni and Ikwerre ethnic groups in Niger delta, Nigeria. Journl of Applied Biosciences 27: 1736-1741 ISSN 1997 5902 Geogrphicl influence on digit rtio (2D:4D): cse study of Andoni nd Ikwerre ethnic groups in Niger delt, Nigeri. Gwunirem, Isrel U 1 nd Ihemelndu,

More information

Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes

Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes originl rticle Serum nesftin-1 levels re decresed in pregnnt women newly dignosed with gesttionl dibetes Esr Nur Ademoglu 1, Suheyl Gorr 2, Muge Keskin 3, Ayse Crlioglu 4, Rifki Ucler 5, Husmettin Erdmr

More information

Synovial sarcoma. Evaluation of prognosis with emphasis on the study of DNA ploidy and proliferation (PCNA and Ki-67) markers

Synovial sarcoma. Evaluation of prognosis with emphasis on the study of DNA ploidy and proliferation (PCNA and Ki-67) markers Anlyticl Cellulr Pthology 16 (1998) 45 62 45 IOS Press Synovil srcom. Evlution of prognosis with emphsis on the study of DNA ploidy nd prolifertion (PCNA nd Ki-67) mrkers José M. Lopes,, Einr Hnnisdl b,

More information

Feeding state and age dependent changes in melaninconcentrating hormone expression in the hypothalamus of broiler chickens

Feeding state and age dependent changes in melaninconcentrating hormone expression in the hypothalamus of broiler chickens Supplementry Mterils Epub: No 2017_23 Vol. 65, 2018 https://doi.org/10.183/bp.2017_23 Regulr pper Feeding stte nd ge dependent chnges in melninconcentrting hormone expression in the hypothlmus of broiler

More information

CME/SAM. Study on Hyperuricemia in HBV-Associated Glomerulonephritis

CME/SAM. Study on Hyperuricemia in HBV-Associated Glomerulonephritis AJCP / Originl Article Study on Hyperuricemi in HBV-Associted Glomerulonephritis Yongze Zhung, MD, PhD, 1 Yingho Yu, MD, PhD, 2 Yingfng Hung, MD, 3 nd Xiorong Zhong, MD 1 From the 1 Deprtment of Nephrology,

More information

Phosphorylated p70s6k in noninvasive low grade urothelial carcinoma of the bladder: correlation with tumor recurrence

Phosphorylated p70s6k in noninvasive low grade urothelial carcinoma of the bladder: correlation with tumor recurrence (2014) 16, 611 617 2014 AJA, SIMM & SJTU. All rights reserved 1008-682X www.sindro.com; www.jndrology.com Mle Helth Open Access ORIGINAL ARTICLE Phosphorylted p70s6k in noninvsive low grde urothelil crcinom

More information

27 June Bmnly L. WALTER ET AL.: RESPONSE OF CERVICAL CANCERS TO IRRADIATION

27 June Bmnly L. WALTER ET AL.: RESPONSE OF CERVICAL CANCERS TO IRRADIATION 27 June 1964 Bmnly MEDICAL JOURNAL L. WALTER ET AL.: RESPONSE OF CERVICAL CANCERS TO IRRADIATION x 1,638.) FIG. 2.-Foci of sme tumour s in Fig. 1 contining vible tumour cells with scnty cytoplsm, reltively

More information

Evaluation of the detection of 14 high-risk human papillomaviruses with HPV 16 and HPV 18 genotyping for cervical cancer screening

Evaluation of the detection of 14 high-risk human papillomaviruses with HPV 16 and HPV 18 genotyping for cervical cancer screening 1332 Evlution of the detection of 14 high-risk humn ppillomviruses with HPV 16 nd HPV 18 genotyping for cervicl cncer screening MEI-LU BIAN, JIAO-YING CHENG, LI MA, XIAO CONG, JUN LIU, YING CHEN nd XI

More information

Association between LAPTM4B gene polymorphism and susceptibility to and prognosis of diffuse large B cell lymphoma

Association between LAPTM4B gene polymorphism and susceptibility to and prognosis of diffuse large B cell lymphoma 264 Assocition between LAPTM4B gene polymorphism nd susceptibility to nd prognosis of diffuse lrge B cell lymphom HUIRONG DING 1*, XIAOJING CHENG 2*, NING DING 3*, ZHIHUA TIAN 1, JUN ZHU 3, CHUNLIAN ZHOU

More information

Classic Papillary Thyroid Carcinoma with Tall Cell Features and Tall Cell Variant Have Similar Clinicopathologic Features

Classic Papillary Thyroid Carcinoma with Tall Cell Features and Tall Cell Variant Have Similar Clinicopathologic Features The Koren Journl of Pthology 2014; 48: 201-208 ORIGINAL ARTICLE Clssic Ppillry Thyroid Crcinom with Tll Cell Fetures nd Tll Cell Vrint Hve Similr Clinicopthologic Fetures Woo Jin Oh 1 Young Sub Lee 1 Uiju

More information

Apoptosis and p53 status predict the efficacy of postoperative administration of UFT in non-small cell lung cancer

Apoptosis and p53 status predict the efficacy of postoperative administration of UFT in non-small cell lung cancer doi: 10.1054/ bjoc.2000.1579, vilble online t http://www.idelibrry.com on http://www.bjcncer.com Apoptosis nd p53 sttus predict the efficcy of postopertive dministrtion of UFT in non-smll cell lung cncer

More information

IGF-I and IGFBP-3 augment transforming growth factor-b actions in human renal carcinoma cells

IGF-I and IGFBP-3 augment transforming growth factor-b actions in human renal carcinoma cells originl rticle http://www.kidney-interntionl.org & Interntionl Society of Nephrology IGF-I nd IGFBP-3 ugment trnsforming growth fctor-b ctions in humn renl crcinom cells AH Rosendhl 1, nd G Forsberg 1

More information

Breast carcinoma grading by histologic features has

Breast carcinoma grading by histologic features has Originl Articles Mitotic Index of Invsive Brest Crcinom Achieving Cliniclly Meningful Precision nd Evluting Tertil Cutoffs John S. Meyer, MD; Eric Costto, PhD; Hns Peter Grf, PhD Context. Mitotic figure

More information

Comparative effectiveness of the tumour diagnostics,

Comparative effectiveness of the tumour diagnostics, Gut, 1987, 28, 323-329 Comprtive effectiveness of the tumour dignostics, C 19-9, C 125 nd crcinoembryonic ntigen in ptients with diseses of the digestive system K SKMOTO, Y HG, R YOSHIMR, H GMI, Y YOKOYM,

More information

TNF-α (pg/ml) IL-6 (ng/ml)

TNF-α (pg/ml) IL-6 (ng/ml) Xio, et l., Supplementry Figure 1 IL-6 (ng/ml) TNF-α (pg/ml) 16 12 8 4 1,4 1,2 1, 8 6 4 2 med Cl / Pm3CSK4 zymosn curdln Poly (I:C) LPS flgelin MALP-2 imiquimod R848 CpG TNF-α (pg/ml) IL-6 (ng/ml) 2 1.6

More information

ENERGY CONTENT OF BARLEY

ENERGY CONTENT OF BARLEY ENERGY CONTENT OF BARLEY VARIATION IN THE DIETARY ENERGY CONTENT OF BARLEY Shwn Firbirn, John Ptience, Hnk Clssen nd Ruurd Zijlstr SUMMARY Formultion of commercil pig diets requires n incresing degree

More information

Lipase and Pancreatic Amylase Activities in Tissues and in Patients with Hyperamylasemia

Lipase and Pancreatic Amylase Activities in Tissues and in Patients with Hyperamylasemia CLINICAL CHEMISTRY Originl Article Lipse nd Pncretic Amylse Activities in Tissues nd in Ptients with Hypermylsemi FRED APPLE, PH.D, PETER BENSON, M.D., LYNNE PREESE, MT, M.B.A., STEVEN EASTEP, M.D., LAURA

More information

Overexpression of FOXM1 Is a Potential Prognostic Marker in Male Breast Cancer

Overexpression of FOXM1 Is a Potential Prognostic Marker in Male Breast Cancer Originl Article Oncol Res Tret 2017;40:167 172 DOI: 10.1159/000458156 Received: September 13, 2016 Accepted: Jnury 26, 2017 Published online: Mrch 15, 2017 Overexpression of FOXM1 Is Potentil Prognostic

More information

Methylation of a Panel of MicroRNA Genes Is a Novel Biomarker for Detection of Bladder Cancer

Methylation of a Panel of MicroRNA Genes Is a Novel Biomarker for Detection of Bladder Cancer EUROPEAN UROLOGY 6 (1) 191 11 vilble t www.sciencedirect.com journl homepge: www.europenurology.com Urothelil Cncer Methyltion of Pnel of MicroRNA Genes Is Novel Biomrker for Detection of Bldder Cncer

More information

Prophylactic effect of neoadjuvant chemotherapy in gastric cancer patients with postoperative complications

Prophylactic effect of neoadjuvant chemotherapy in gastric cancer patients with postoperative complications Gstric Cncer (2018) 21:703 709 https://doi.org/10.1007/s10120-017-0781-y ORIGINAL ARTICLE Prophylctic effect of neodjuvnt chemotherpy in gstric cncer ptients with postopertive complictions Kojiro Eto 1

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.

More information

MOLECULAR AND CLINICAL ONCOLOGY 5: , 2016

MOLECULAR AND CLINICAL ONCOLOGY 5: , 2016 MOLECULAR AND CLINICAL ONCOLOGY 5: 429-436, 2016 Improvement of survivl with postmstectomy rdiotherpy in ptients with 1-3 positive xillry lymph nodes: A systemtic review nd met-nlysis of the current literture

More information

EFFECTS OF INGREDIENT AND WHOLE DIET IRRADIATION ON NURSERY PIG PERFORMANCE

EFFECTS OF INGREDIENT AND WHOLE DIET IRRADIATION ON NURSERY PIG PERFORMANCE Swine Dy 21 EFFECTS OF INGREDIENT AND WHOLE DIET IRRADIATION ON NURSERY PIG PERFORMANCE J. M. DeRouchey, M. D. Tokch, J. L. Nelssen, R. D. Goodbnd, S. S. Dritz 1, J. C. Woodworth, M. J. Webster, B. W.

More information

Single-Molecule Studies of Unlabelled Full-Length p53 Protein Binding to DNA

Single-Molecule Studies of Unlabelled Full-Length p53 Protein Binding to DNA Single-Molecule Studies of Unlbelled Full-Length p53 Protein Binding to DNA Philipp Nuttll, 1 Kidn Lee, 2 Pietro Ciccrell, 3 Mrco Crminti, 3 Giorgio Ferrri, 3 Ki- Bum Kim, 2 Tim Albrecht 1* 1 Imperil College

More information

Supplementary figure 1

Supplementary figure 1 Supplementry figure 1 Dy 8 post LCMV infection Vsculr Assoc. Prenchym Dy 3 post LCMV infection 1 5 6.7.29 1 4 1 3 1 2 88.9 4.16 1 2 1 3 1 4 1 5 1 5 1.59 5.97 1 4 1 3 1 2 21.4 71 1 2 1 3 1 4 1 5 1 5.59.22

More information

Effects of modified FOLFOX-6 chemotherapy on cellular immune function in patients with gastric cancer

Effects of modified FOLFOX-6 chemotherapy on cellular immune function in patients with gastric cancer ONCOLOGY LETTERS 15: 8635-8640, 2018 Effects of modified FOLFOX-6 chemotherpy on cellulr immune function in ptients with gstric cncer LIANG WANG 1, DONGER ZHOU 1, HAITAO REN 2 nd YAN CHEN 3 Deprtments

More information

A proliferation-inducing ligand (APRIL) in neutrophils of patients with oral cavity squamous cell carcinoma

A proliferation-inducing ligand (APRIL) in neutrophils of patients with oral cavity squamous cell carcinoma Eur. Cytokine Netw. Vol. 23 n 3, July-August-September 212, 93-1 93 RESEARCH ARTICLE A prolifertion-inducing lignd (APRIL) in neutrophils of ptients with orl cvity squmous cell crcinom Ew Jbłońsk 1, Ntli

More information

Using proliferative markers and Oncotype DX in therapeutic decision-making for breast cancer: the B.C. experience

Using proliferative markers and Oncotype DX in therapeutic decision-making for breast cancer: the B.C. experience ORIGINAL ARTICLE Using prolifertive mrkers nd Oncotype DX in therpeutic decision-mking for brest cncer: the B.C. experience E. Bxter bsc,* L. Gondr btech pdc, C. Lohrisch md, S. Chi md, K. Gelmon md, M.

More information

WSU Tree Fruit Research and Extension Center, Wenatchee (509) ext. 265;

WSU Tree Fruit Research and Extension Center, Wenatchee (509) ext. 265; FINAL REPORT WTFRC Project # AH-1-5 WSU Project # 13C-355-3 Project title: PI: Orgniztion: Coopertors: of Sunburn in Apples with RAYNOX Lrry Schrder, Horticulturist WSU Tree Fruit Reserch nd Extension

More information

MOLECULAR AND CLINICAL ONCOLOGY 7: , 2017

MOLECULAR AND CLINICAL ONCOLOGY 7: , 2017 MOLECULAR AND CLINICAL ONCOLOGY 7: 787-797, 2017 Evlution of epiderml growth fctor receptor serum levels nd their ssocition with clinicopthologicl chrcteristics in ptients with colorectl cncer MEHMET KARABULUT

More information

PD L1 expression is associated with advanced non small cell lung cancer

PD L1 expression is associated with advanced non small cell lung cancer ONCOLOGY LETTERS 12: 921-927, 2016 PD L1 expression is ssocited with dvnced non smll cell lung cncer ZHIQUAN CHEN 1 3, JIANDONG MEI 3 5, LUNXU LIU 4,5, GUOCHEN WANG 2, ZUOSHENG LI 2, JINGPU HOU 2, QIUYANG

More information

Expression of long chain fatty acid receptor GPR40 is associated with cancer progression in colorectal cancer: A retrospective study

Expression of long chain fatty acid receptor GPR40 is associated with cancer progression in colorectal cancer: A retrospective study ONCOLOGY LETTERS 15: 8641-8646, 2018 Expression of long chin ftty cid receptor GPR40 is ssocited with cncer progression in colorectl cncer: A retrospective study CHIE NAKASHIMA 1, KISHI SHINGO 1, RINA

More information

Rheumatoid-susceptible alleles of HLA-DRB 1 are genetically recessive to non-susceptible alleles in the progression of bone destruction in the wrists

Rheumatoid-susceptible alleles of HLA-DRB 1 are genetically recessive to non-susceptible alleles in the progression of bone destruction in the wrists Annls of the Rheumtic Diseses 1994; 53: 587-592 587 Deprtment of Orthopedic Surgery, Knsi Medicl University, Otokoym Hospitl, Kyoto, Jpn Y Tod Y Mori Deprtment of Orthopedic Surgery, Knsi Medicl University,

More information

Differentiation of malignant from normal and reactive mesothelial cells by the argyrophil technique for

Differentiation of malignant from normal and reactive mesothelial cells by the argyrophil technique for Thorx 1988;43:366-370 Differentition of mlignnt from norml nd rective mesothelil cells by the rgyrophil technique for nucleolr orgniser region ssocited proteins JON G AYRES, JOHN G CROCKER, NCHOLAS Q SKLBECK

More information

Clinical Study Report Synopsis Drug Substance Naloxegol Study Code D3820C00018 Edition Number 1 Date 01 February 2013 EudraCT Number

Clinical Study Report Synopsis Drug Substance Naloxegol Study Code D3820C00018 Edition Number 1 Date 01 February 2013 EudraCT Number EudrCT Number 2012-001531-31 A Phse I, Rndomised, Open-lbel, 3-wy Cross-over Study in Helthy Volunteers to Demonstrte the Bioequivlence of the Nloxegol 25 mg Commercil nd Phse III Formultions nd to Assess

More information

METHOD 4010 SCREENING FOR PENTACHLOROPHENOL BY IMMUNOASSAY

METHOD 4010 SCREENING FOR PENTACHLOROPHENOL BY IMMUNOASSAY METHOD 4010 SCREENING FOR PENTACHLOROPHENOL BY IMMUNOASSAY 1.0 SCOPE AND APPLICATION 1.1 Method 4010 is procedure for screening solids such s soils, sludges, nd queous medi such s wste wter nd lechtes

More information

Effects of Bevacizumab on Autocrine VEGF Stimulation in Bladder Cancer Cell Lines

Effects of Bevacizumab on Autocrine VEGF Stimulation in Bladder Cancer Cell Lines Urologi Interntionlis Originl Pper DOI: 1.1159/32195 Received: April 26, 21 Accepted fter revision: October 3, 21 Published online: Jnury 8, 211 Effects of Bevcizumb on Autocrine VEGF Stimultion in Bldder

More information

Int J Clin Exp Med 2018;11(8): /ISSN: /IJCEM Jun Luo, Peng Hao, Xuesong Gao, Yuchuan Wang, Ruiqiang Sun

Int J Clin Exp Med 2018;11(8): /ISSN: /IJCEM Jun Luo, Peng Hao, Xuesong Gao, Yuchuan Wang, Ruiqiang Sun Int J Clin Exp Med 2018;11(8):8479-8486 www.ijcem.com /ISSN:1940-5901/IJCEM0068421 Originl Article Dexmedetomidine pretretment llevites cerebrl ischemi-reperfusion injury in rts through resisting oxidtive

More information

Protective effect of rosuvastatin treatment by regulating oxidized low-density lipoprotein expression in a rat model of liver fibrosis

Protective effect of rosuvastatin treatment by regulating oxidized low-density lipoprotein expression in a rat model of liver fibrosis BIOMEDICAL REPORTS 5: 311-316, 2016 Protective effect of rosuvsttin tretment by regulting oxidized low-density lipoprotein expression in rt model of liver fibrosis SHUIPING YU 1, XUELING ZHOU 1, BINGZONG

More information

Hypertension, hyperinsulinaemia and obesity in middle-aged Finns with impaired glucose tolerance

Hypertension, hyperinsulinaemia and obesity in middle-aged Finns with impaired glucose tolerance Journl of Humn Hypertension (1998) 12, 265 269 1998 Stockton Press. All rights reserved 0950-9240/98 $12.00 ORIGINAL ARTICLE Hypertension, hyperinsulinemi nd obesity in middle-ged Finns with impired glucose

More information

Age related differences in prognosis and prognostic factors among patients with epithelial ovarian cancer

Age related differences in prognosis and prognostic factors among patients with epithelial ovarian cancer MOLECULAR AND CLINICAL ONCOLOGY 9: 329-334, 2018 Age relted differences in prognosis nd prognostic fctors mong ptients with epithelil ovrin cncer KENJI YOSHIKAWA, TAKESHI FUKUDA, RYO UEMURA, HIROAKI MATSUBARA,

More information

Effects of physical exercise on working memory and prefrontal cortex function in post-stroke patients

Effects of physical exercise on working memory and prefrontal cortex function in post-stroke patients Effects of physicl exercise on working memory nd prefrontl cortex function in post-stroke ptients M Moriy, C Aoki, K Sktni Grdute School of Helth Sciences Reserch, Mjor of Physicl Therpy, TeikyoHeisei

More information

Opioid Use and Survival at the End of Life: A Survey of a Hospice Population

Opioid Use and Survival at the End of Life: A Survey of a Hospice Population 532 Journl of Pin nd Symptom Mngement Vol. 32 No. 6 December 2006 NHPCO Originl Article Opioid Use nd Survivl t the End of Life: A Survey of Hospice Popultion Russell K. Portenoy, MD, Un Sibircev, BA,

More information

Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells

Correlation of ERK/MAPK signaling pathway with proliferation and apoptosis of colon cancer cells ONCOLOGY LETTERS Correltion of ERK/MAPK signling pthwy with prolifertion nd poptosis of colon cncer cells GANG ZHOU, JING YANG nd PENG SONG Deprtment of Medicl Oncology, The Second Medicl Centre, Chinese

More information

Effect of TCN2 776C G on vitamin B 12 cellular availability. end-stage renal disease patients

Effect of TCN2 776C G on vitamin B 12 cellular availability. end-stage renal disease patients Kidney Interntionl, Vol. 64 (2003), pp. 1095 1100 Effect of TCN2 776C G on vitmin B 12 cellulr vilbility in end-stge renl disese ptients MANUELA FÖDINGER, MARIO VEITL, SONJA SKOUPY, JADWIGA WOJCIK, CLAUDIA

More information

Relationship between serum irisin, glycemic indices, and renal function in type 2 diabetic patients

Relationship between serum irisin, glycemic indices, and renal function in type 2 diabetic patients J Renl Inj Prev. 2017; 6(2): 88-92. http://journlrip.com Journl of Renl Injury Prevention DOI: 10.15171/jrip.2017.17 Reltionship between serum irisin, glycemic indices, nd renl function in type 2 dibetic

More information

Predictors of Hospitalization in Male Marine Corps Recruits with Exertional Heat Illness

Predictors of Hospitalization in Male Marine Corps Recruits with Exertional Heat Illness MILITARY MEDICINE, 169, 3:169, 2004 Predictors of Hospitliztion in Mle Mrine Corps Recruits with Exertionl Het Illness Gurntor: COL John W. Grdner, MC FS USA Contributors: Shilp Hkre, DrPH; COL John W.

More information

Significance of Silver Binding Nucleolar Organizer Regions in Oral Squamous Cell Carcinomas

Significance of Silver Binding Nucleolar Organizer Regions in Oral Squamous Cell Carcinomas Originl Article DOI: 10.17354/ijss/2016/147 Significnce of Silver Binding Nucleolr Orgnizer Regions in Orl Squmous Cell Crcinoms Ritu Shrm 1, Gurv Kumr 2 1 Assistnt Professor, Deprtment of Pthology, Hind

More information

Original Article CD40-1C>T polymorphism and the risk of lung cancer in a Chinese population

Original Article CD40-1C>T polymorphism and the risk of lung cancer in a Chinese population Int J Clin Exp Pthol 2015;8(11):15163-15169 www.ijcep.com /ISSN:1936-2625/IJCEP0015790 Originl Article CD40-1C>T polymorphism nd the risk of lung cncer in Chinese popultion Gng Zhou 1*, Ying Wng 1,2*,

More information

Analysis of Risk Factors for the Development of Incisional and Parastomal Hernias in Patients after Colorectal Surgery

Analysis of Risk Factors for the Development of Incisional and Parastomal Hernias in Patients after Colorectal Surgery Originl Article Journl of the Koren Society of J Koren Soc Coloproctol 2012;28(6):299-303 http://dx.doi.org/10.3393/jksc.2012.28.6.299 pissn 2093-7822 eissn 2093-7830 Anlysis of Risk Fctors for the Development

More information

Utilization of dental services in Southern China. Lo, ECM; Lin, HC; Wang, ZJ; Wong, MCM; Schwarz, E

Utilization of dental services in Southern China. Lo, ECM; Lin, HC; Wang, ZJ; Wong, MCM; Schwarz, E Title Utiliztion of dentl services in Southern Chin Author(s) Lo, ECM; Lin, HC; Wng, ZJ; Wong, MCM; Schwrz, E Cittion Journl Of Dentl Reserch, 2001, v. 80 n. 5, p. 1471-1474 Issued Dte 2001 URL http://hdl.hndle.net/10722/53200

More information

The potential future of targeted radionuclide therapy: implications for occupational exposure? P. Covens

The potential future of targeted radionuclide therapy: implications for occupational exposure? P. Covens The potentil future of trgeted rdionuclide therpy: implictions for occuptionl exposure? Introduction: Trgeted Rdionuclide Therpy (TRT) Systemic tretment Molecule lbelled with rdionuclide delivers toxic

More information

Association on polymorphisms in LncRNA HOTAIR and susceptibility to HNSCC in Chinese population

Association on polymorphisms in LncRNA HOTAIR and susceptibility to HNSCC in Chinese population Europen Review for Medicl nd Phrmcologicl Sciences 2018; 22: 702-706 Assocition on polymorphisms in LncRNA HOTAIR nd susceptibility to HNSCC in Chinese popultion B. WU 1, J. LIU 2, B. WANG 2, X. LIAO 1,

More information

Effects of Weight Reduction on Serum Vaspin Concentrations in Obese Subjects: Modification by Insulin Resistance

Effects of Weight Reduction on Serum Vaspin Concentrations in Obese Subjects: Modification by Insulin Resistance nture publishing group rticles Effects of Weight Reduction on Serum Vspin Concentrtions in Obese Subjects: Modifiction by Insulin Resistnce Hye M. Chng 1, He J. Lee 1, Hye S. Prk 1, Je H. Kng 2, Kyung

More information

Inhaled Corticosteroid Is Associated With an Increased Risk of TB in Patients With COPD

Inhaled Corticosteroid Is Associated With an Increased Risk of TB in Patients With COPD CHEST Originl Reserch Inhled Corticosteroid Is Associted With n Incresed Risk of TB in Ptients With COPD Jung-Hyun Kim, MD ; Ji-Soo Prk, MD ; Kyung-Ho Kim, MD ; Hye-Cheol Jeong, MD ; Eun-Kyung Kim, MD

More information

DR. MARC PAGÈS Project Manager R&D Biologicals - Coccidia Projects, HIPRA

DR. MARC PAGÈS Project Manager R&D Biologicals - Coccidia Projects, HIPRA DR. MARC PAGÈS Project Mnger R&D Biologicls - Coccidi Projects, HIPRA Dr. Mrc Pgès Bosch otined Microiology nd Genetics degree t the University of Brcelon in 1998. He otined his PhD working on the synptoneml

More information

Journal of Hainan Medical University 2017; 23(2): Journal of Hainan Medical University.

Journal of Hainan Medical University 2017; 23(2): Journal of Hainan Medical University. Journl of Hinn Medicl University 2017; 23(2): 151-155 151 Journl of Hinn Medicl University http://www.hnykdxxb.com Reltionship between DEXA bone minerl density mesurement results nd serum cytokines s well

More information

Precipitins and specific IgG antibody to

Precipitins and specific IgG antibody to 48 Thorx 1992;47:48-52 Precipitins nd specific IgG ntibody to Aspergillus fumigtus in chest unit popultion Deprtment of Immunology nd Osler Chest Unit, Churchill Hospitl, Oxford OX3 7LJ J A Fux D J Shle

More information

Characteristics of hip involvement in patients with ankylosing spondylitis in Korea

Characteristics of hip involvement in patients with ankylosing spondylitis in Korea ORIGINAL ARTICLE Koren J Intern Med 2017;32:158-164 Chrcteristics of hip involvement in ptients with nkylosing spondylitis in Kore Hyemin Jeong, Yeong Hee Eun, In Young Kim, Hyungjin Kim, Jejoon Lee, Eun-Mi

More information

Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion

Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion PO Box 2345, Beijing 100023, Chin World J Gstroenterol 2005;11(29):4524-4529 www.wjgnet.com World Journl of Gstroenterology ISSN 1007-9327 wjg@wjgnet.com ELSEVIER 2005 The WJG Press nd Elsevier Inc. All

More information

World Journal of Gastroenterology

World Journal of Gastroenterology ISSN 17-937 (print) ISSN 19-84 (online) World Journl of Gstroenterology World J Gstroenterol 17 My 7; 3(17): 311-3194 Published by Bishideng Publishing Group Inc S Contents Weekly Volume 3 Number 17 My

More information

Keywords: Anti-human leukocyte antigen-donor specific antibody; Kidney transplantation; Luminex Single Antigen assay; Allograft biopsy

Keywords: Anti-human leukocyte antigen-donor specific antibody; Kidney transplantation; Luminex Single Antigen assay; Allograft biopsy ORIGINAL ARTICLE Koren J Intern Med 18;33:157-167 https://doi.org/1.394/kjim.16.17 Clinicl significnce of the presence of ntihumn leukocyte ntigen-donor specific ntibody in kidney trnsplnt recipients with

More information

Reduced expression of cytokeratin 4 and 13 is a valuable marker for histologic grading of esophageal squamous intraepithelial neoplasia

Reduced expression of cytokeratin 4 and 13 is a valuable marker for histologic grading of esophageal squamous intraepithelial neoplasia J Med Dent Sci 2012; 59: 17-28 Originl Article Reduced expression of cytokertin 4 nd 13 is vlule mrker for histologic grding of esophgel squmous intrepithelil neoplsi Mski Tkshim 1), Hiroshi Kwchi 1),

More information

XII. HIV/AIDS. Knowledge about HIV Transmission and Misconceptions about HIV

XII. HIV/AIDS. Knowledge about HIV Transmission and Misconceptions about HIV XII. HIV/AIDS Knowledge bout HIV Trnsmission nd Misconceptions bout HIV One of the most importnt prerequisites for reducing the rte of HIV infection is ccurte knowledge of how HIV is trnsmitted nd strtegies

More information