Lysine analogue of Polymyxin B as a significant opportunity for photodynamic antimicrobial chemotherapy
|
|
- David Bennett
- 5 years ago
- Views:
Transcription
1 SUPPORTING INFORMATION Lysine analogue of Polymyxin B as a significant opportunity for photodynamic antimicrobial chemotherapy Florent Le Guern, Tan-Sothea Ouk *, Catherine Ouk, Regis Vanderesse +, Yves Champavier, Emilie Pinault, Vincent Sol * Université de Limoges, Laboratoire de Chimie des Substances Naturelles, EA 1069, 123 Avenue Albert Thomas, Limoges Cedex, France. Université de Limoges, BISCEm, Limoges, France + Université de Lorraine, Laboratoire de Chimie Physique Macromoléculaire (LCPM), UMR 7375 CNRS, ENSIC, 1 rue Grandville, Nancy, France 1
2 Tables of Contents Figure S1. Synthetic route of 5Lys based on the previous work... 3 Figure S2. MS and 1 H NMR analysis of 1Lys Figure S3. HRMS analysis of 5Lys... 5 Figure S4. 1 H NMR analysis of 5Lys... 6 Figure S5. 1 H COSY NMR analysis of 5Lys... 7 Figure S6. 1 H TOCSY NMR analysis of 5Lys... 8 Figure S7. Absorbance spectra of 5Lys and 5 in MeOH (4.3 µm)... 9 Figure S8. Absorption, fluorescence, and phosphorescence spectra Figure S9. Confocal laser scanning microscopy imaging of NHDF after incubation with 5Lys 11 Figure S10. Additional confocal laser scanning microscopy imaging of NHDF and bacteria Table S1. Fluorescence and phosphorescence properties Table S2. IC 50 (um) of the different molecules
3 Figure S1. Synthetic route of 5Lys based on the previous work (Le Guern et al., 2017). a) Propionic acid, 3 h, 140 C. b) Iodomethane, anhydrous DMF, 1 h, 140 C. c) 25% TFA/H 2 O, 5 h, 100 C. d) 6-maleimidohexanoic acid, HCTU, DIEA, anhydrous DMF, 24 h, RT. e) PBS ph 6.5, 24 h, RT. 3
4 Figure S2. MS and 1 H NMR (in DMSO-d 6 ) analysis of 1Lys. 4
5 Figure S3. HRMS analysis of 5Lys 5
6 Figure S4. 1 H NMR (in DMSO-d 6 ) analysis of 5Lys 6
7 Figure S5. 1 H COSY NMR analysis of 5Lys 7
8 Figure S6. 1 H TOCSY NMR analysis of 5Lys 8
9 1 5 5Lys Absorbance (A.U.) Wavelength (nm) Figure S7. Absorbance spectra of 5Lys and 5 in MeOH (4.3 µm) 9
10 Absorbance (a.u) A TMPyP 5 5Lys Fluorescence intensity (a.u) 2,5x10 7 B 2,0x10 7 1,5x10 7 1,0x10 7 5,0x10 6 TMPyP 5 5Lys Wavelength (nm) Phosphorescence intensity (a.u) 0,0016 0,0014 0,0012 0,0010 0,0008 0,0006 0,0004 0,0002 C 0, Wavelength (nm) TMPyP 5 5Lys 0, Wavelength (nm) Figure S8. Absorption (A) ([TMPyP] = 13.8 µm, [5] = 16 µm, [5Lys] = 16 µm), fluorescence (B), and phosphorescence (C) spectra of TMPyP, 5 and 5Lys in D 2 O (λ exc = 425 nm, A 425 ~ 0.2). Table S1. Fluorescence and phosphorescence properties of conjugate TMPyP, 5 and 5Lys in D 2 O. a TPP as standard (Φ F (toluene) = 0.11) (Redmond and Gamlin, 1999), b TMPyP as standard (Φ (D 2 O) = 0.90) (Redmond and Gamlin, 1999), c Literature value ~ 67 µs in D 2 O (Ogilby and Foote, 1983; Wilkinson et al., 1995).(λ exc = 425 nm) compd λ F max (nm) φ F a φ τ F (ns) τ (µs) c TMPyP b Lys
11 Figure S9. Confocal laser scanning microscopy imaging of NHDF after incubation with 5Lys. Cells were suspended in a solution of 5Lys (1 µm) for 30 min at 37 C, then washed 3 times with PBS. 11
12 IC 50 (um) Light NHDF Dark 1Lys Lys Table S2. IC 50 (um) of the different molecules after light irradiation (white LED, 35 J/cm²) and in the dark. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. 48h prior to experiments, 100 µl per well of cell suspension at 0.5 x 10 5 cells per ml were cultured in 96-well microplates in complete FGM culture medium (Lonza, Germany). Culture medium was removed and cells were incubated in the dark with different concentrations of PS, ranging from 5 nm to 5000 nm (in PBS 1X). After 2 hours of incubation at 37 C in the dark, cells were washed three times with PBS, and then 100 µl of fresh FGM culture medium were added in each well. One plate was exposed to a white light irradiation (4.83 mw/cm²) for 2 hours (35 J/cm²) at 37 C and then was placed at 37 C in the dark. Another plate was kept in the dark at 37 C without irradiation (dark condition). After 24 hours at 37 C, the MTT was added at a final concentration of 0.5 mg/ml in each well and cells were incubated at 37 C for 2 hours. Then, the culture medium was harvested and replaced by 100 µl of DMSO in each well in order to dissolve formazan crystals. The absorbance was measured at 595 nm using a Bio-Rad imark microplate reader. 12
13 Figure S10. Additional confocal laser scanning microscopy imaging of NHDF and bacteria (P. aeruginosa and S. aureus) after incubation with 5Lys. NHDF cells (5 000 cells) were seeded in a 8-well Lab-Tek TM II chamber coverglass (NUNC) in complete FBM culture medium for 48 hours at 37 C CFU were prepared and washed three times with PBS CFU were directly added to each well. After 30 min of incubation at 37 C, the supernatant was removed and the wells were washed once with PBS. Then, 500 µl of PS solutions (1 µm in PBS) were deposited after 30 min at 37 C in the dark, three washing steps were done, and then confocal microscopy imaging was carried out as described before, with a 40X objective lens. 13
Electronic Supporting Information for
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Electronic Supporting Information for Rhodamine based Turn-On Fluorescent Probe for Pb(II)
More informationB16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small cell lung cancer
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Experimental Methods Cell culture B16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small
More informationA novel quinoline-based two-photon fluorescent probe for detecting Cd 2+ in vitro and in vivo
Supporting Information A novel quinoline-based two-photon fluorescent probe for detecting Cd 2+ in vitro and in vivo Yiming Li, a,b Hanbao Chong, a Xiangming Meng,* a Shuxin Wang, a Manzhou Zhu a and Qingxiang
More informationbio-mof-1 DMASM Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM.
bio-mof-1 Transmittance bio-mof-1 DMASM DMASMI 2000 1500 1000 500 Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM. Intensity (a.u.) bio-mof-1 DMASM as
More informationA Single fluorescent probe for Dual-imaging Viscosity and H 2 O 2 in Mitochondria with Different Fluorescence Signals in Living Cells
Supporting Information for A Single fluorescent probe for Dual-imaging Viscosity and H 2 O 2 in Mitochondria with Different Fluorescence Signals in Living Cells Mingguang Ren, Beibei Deng, Kai Zhou, Xiuqi
More informationSupporting Information Nitric oxide releasing photoresponsive nanohybrids as excellent therapeutic agent for cervical cancer cell lines
upporting Information itric oxide releasing photoresponsive nanohybrids as excellent therapeutic agent for cervical cancer cell lines Priya udhesh a, Kaviyarasan Tamilarasan a, Palaniappan Arumugam a and
More informationSupporting Information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Supporting Information Enzyme-activatable Probe with a Self-immolative Linker for Rapid and Sensitive
More informationCarbon-1 versus carbon-3 linkage of D-galactose to porphyrins: Synthesis, uptake, and photodynamic efficiency
Supporting information for Carbon-1 versus carbon-3 linkage of D-galactose to porphyrins: Synthesis, uptake, and photodynamic efficiency Patrícia M. R. Pereira #, Waqar Rizvi #, N. V. S. Dinesh K. Bhupathiraju,
More informationChemical composition, antioxidant and antibacterial activities of Tamarix balansae J. Gay aerial parts
SUPPLEMENTARY MATERIAL Chemical composition, antioxidant and antibacterial activities of Tamarix balansae J. Gay aerial parts Abbes Benmerache a, Mounira Benteldjoune a, Abdulmagid Alabdul Magid b, Amin
More informationPhotoswitchable micelles for the control of singlet-oxygen generation in. photodynamic therapies
Supporting Information for Photoswitchable micelles for the control of singlet-oxygen generation in photodynamic therapies Yan Zhai, Henk J. Busscher,,* Yong Liu, Zhenkun Zhang, Theo G. van Kooten, Linzhu
More informationSupporting Information
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2018 Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 2016. Supporting Information
More informationNature Protocols: doi: /nprot Supplementary Figure 1. Fluorescent titration of probe CPDSA.
Supplementary Figure 1 Fluorescent titration of probe CPDSA. Fluorescent titration of probe CPDSA (10 um) upon addition of GSH in HEPES (10 mm, ph = 7.4) containing 10% DMSO. Each spectrum was recorded
More information2,6,9-Triazabicyclo[3.3.1]nonanes as overlooked. amino-modification products by acrolein
Supplementary Information 2,6,9-Triazabicyclo[3.3.1]nonanes as overlooked amino-modification products by acrolein Ayumi Tsutsui and Katsunori Tanaka* Biofunctional Synthetic Chemistry Laboratory, RIKEN
More informationSUPPORTING INFORMATION. For. ACS Applied Materials & Interfaces
SUPPRTIG IFRMATI For ACS Applied Materials & Interfaces S-1 Specific Fluorescence Probes for Lipid Droplets Based on Simple AIEgens Zhiming Wang,,,, # Chen Gui,,, Engui Zhao,, Jing Wang, # Xiaodong Li,
More informationA Photostable AIE Luminogen for Specific Mitochondrial
SUPPORTING INFORMATION A Photostable AIE Luminogen for Specific Mitochondrial Imaging and Tracking Chris Wai Tung Leung,,# Yuning Hong,,# Sijie Chen, Engui Zhao, Jacky Wing Yip Lam, and Ben Zhong Tang
More informationSupporting Information. Silver Nanoparticle-Gated Mesoporous Silica-Coated Gold Nanorods. Low Premature Release and Multifunctional
Supporting Information Silver Nanoparticle-Gated Mesoporous Silica-Coated Gold Nanorods (AuNR@MS@AgNPs): Low Premature Release and Multifunctional Cancer Theranostic Platform Zhehua Zhang, a, Changhui
More informationInhibition of TGFβ enhances chemotherapy action against triple negative breast cancer by abrogation of
SUPPLEMENTAL DATA Inhibition of TGFβ enhances chemotherapy action against triple negative breast cancer by abrogation of cancer stem cells and interleukin-8 Neil E. Bhola 1, Justin M. Balko 1, Teresa C.
More informationSupporting Information
Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 212. Supporting Information for Adv. Funct. Mater., DOI:.2/adfm.2122233 MnO Nanocrystals: A Platform for Integration of MRI and Genuine
More informationData Sheet. Fluorogenic HDAC 8 Assay Kit Catalog #: 50068
Data Sheet Fluorogenic HDAC 8 Assay Kit Catalog #: 50068 DESCRIPTION: The Fluorogenic HDAC 8 Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) 8 activity for screening
More information8. CHAPTER IV. ANTICANCER ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES
8. CHAPTER IV. ANTICANCER ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES 8.1. Introduction Nanobiotechnology, an emerging field of nanoscience, utilizes nanobased-systems for various biomedical applications.
More informationab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric)
Version 10 Last updated 19 December 2017 ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric) For the measurement of triglycerides in various samples. This product is for research
More informationFluorescent probes for detecting monoamine oxidase activity and cell imaging
Fluorescent probes for detecting monoamine oxidase activity and cell imaging Xuefeng Li, Huatang Zhang, Yusheng Xie, Yi Hu, Hongyan Sun *, Qing Zhu * Supporting Information Table of Contents 1. General
More informationSupporting Information
Supporting Information Toward High-Efficient Red Emissive Carbon Dots: Facile Preparation, Unique Properties, and Applications as Multifunctional Theranostic Agents Shan Sun,, Ling Zhang, Kai Jiang, Aiguo
More informationDevelopment of a near-infrared fluorescent probe for monitoring hydrazine in serum and living cells
Supporting Information for Development of a near-infrared fluorescent probe for monitoring hydrazine in serum and living cells Sasa Zhu, Weiying Lin,* Lin Yuan State Key Laboratory of Chemo/Biosensing
More informationSupporting Information Appendix
Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes Alina Y. Rwei, Jung-Jae Lee, Changyou Zhan, Qian Liu, Meryem T. Ok, Sahadev A. Shankarappa, Robert Langer, Daniel
More informationA highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae
Electronic Supporting Information highly selective IE fluorogen for lipid droplet imaging in live cells and green algae Erjing Wang, ab Engui Zhao, ab Yuning Hong, ab Jacky W. Y. Lam, ab and en Zhong Tang*
More informationSupporting Information
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2017 Supporting Information Cancer-mitochondria-targeted photodynamic therapy with supramolecular
More informationIN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS
CHAPTER 3 IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS 3. INTRODUCTION Plants are the basic source of knowledge of modern medicine. Almost all the parts of the plant, namely
More informationThe effect of insulin on chemotherapeutic drug sensitivity in human esophageal and lung cancer cells
The effect of insulin on chemotherapeutic drug sensitivity in human esophageal and lung cancer cells Published in: Natl Med J China, February 10, 2003; Vol 83, No 3, Page 195-197. Authors: JIAO Shun-Chang,
More informationUltrapure laser-synthesized Si-based nanomaterials for biomedical. applications: in vivo assessment of safety and biodistribution
Supplementary Information Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution Tarek Baati, 1 Ahmed Al-Kattan, 2 Marie-Anne Esteve,
More informationSupplementary Information. Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food
Supplementary Information Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena and Debendra K. Sahoo* Biochemical
More informationSupporting Information For
Supporting Information For MicroRNA-Catalyzed Cancer Therapeutics Based on DNA-Programmed Nanoparticle Complex Xucheng Luo, 1 Zhi Li, 1 Ganglin Wang, 1 Xuewen He, 2,3 Xiaoqin Shen, 1 Quanhong Sun, 1 Li
More informationMetal swap between Zn 7 metallothionein 3 and amyloid β Cu protects against amyloid β toxicity
Metal swap between Zn 7 metallothionein 3 and amyloid β Cu protects against amyloid β toxicity Supplementary Information Gabriele Meloni 1, Vanessa Sonois 2,3, Tamara Delaine 2, Luc Guilloreau 2, Audrey
More informationElectronic Supplementary Information (ESI) A unique dansyl-based chromogenic chemosensor for rapid and ultrasensitive hydrazine detection
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information (ESI) A unique dansyl-based chromogenic
More informationDual-site Controlled and Lysosome-targeted ICT-PET-FRET. Fluorescent Probe for Monitoring ph Changes in Living Cells
Supporting information for Dual-site Controlled and Lysosome-targeted ICT-PET-FRET Fluorescent Probe for Monitoring ph Changes in Living Cells Baoli Dong, Xuezhen Song, Chao Wang, Xiuqi Kong, Yonghe Tang
More informationab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric)
ab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of uric acid in various samples. This product is for research use only and
More informationAnti-cancer activity of Aya Thambira Chendooram (ATC) in in-vitro cell line against Breast Carcinoma
International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 5, Issue 1-2018 Research Article DOI: http://dx.doi.org/10.22192/ijarbs.2018.05.01.010
More informationSupporting Information. A two-photon ratiometric fluorescent probe for imaging. carboxylesterase 2 in living cells and tissues
Supporting Information A two-photon ratiometric fluorescent probe for imaging carboxylesterase 2 in living cells and tissues Qiang Jin,, Lei Feng,, Dan-Dan Wang, Zi-Ru Dai, Ping Wang, Li-Wei Zou, Zhi-Hong
More informationSupplementary data file. Three-in-one type fluorescent sensor based on pyrene pyridoxal cascade for the selective
Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is The Royal Society of Chemistry 2017 Supplementary data file Three-in-one type fluorescent sensor based on pyrene pyridoxal
More informationSupporting Information
Electronic Supplementary Material (ESI) for MedChemComm. This journal is The Royal Society of Chemistry 2015 Supporting Information Synthesis and Biological Evaluation of D- gluconhydroximo-1, 5-lactam
More informationMTS assay in THP-1 cells
Project: VIGO MTS assay in THP-1 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
More informationASSESSMENT OF CELLULAR OXYGEN GRADIENTS WITH A PANEL OF PHOSPHORESCENT OXYGEN-SENSITIVE PROBES
ASSESSMENT OF CELLULAR OXYGEN GRADIENTS WITH A PANEL OF PHOSPHORESCENT OXYGEN-SENSITIVE PROBES Ruslan I. Dmitriev, Alexander V. Zhdanov, Greg Jasionek, Dmitri B. Papkovsky Biochemistry Department, University
More informationComparison of Cytotoxic Activity of Anticancer Drugs against Various Human Tumor Cell Lines Using In Vitro Cell-Based Approach
International journal of Biomedical science ORIGINAL ARTICLE Comparison of Cytotoxic Activity of Anticancer Drugs against Various Human Tumor Cell Lines Using In Vitro Cell-Based Approach Leila Florento,
More informationHCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation
SUPPLEMENTARY INFORMATION Materials and Methods Human cell lines and culture conditions HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation in exon 20 of BRCA1
More informationStudy of structure-bioactivity relationship of three new pyridine Schiff bases:
Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2018 Study of structure-bioactivity
More informationThe Annexin V Apoptosis Assay
The Annexin V Apoptosis Assay Development of the Annexin V Apoptosis Assay: 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium dependent manner.
More informationSensoLyte 520 Cathepsin K Assay Kit *Fluorimetric*
SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric* Catalog # 72171 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Cathepsin K activity. Enhanced Value: Ample
More informationGuajavadimer A, a dimeric caryophyllene-derived meroterpenoid with a new carbon skeleton from the leaves of Psidium guajava.
Guajavadimer A, a dimeric caryophyllene-derived meroterpenoid with a new carbon skeleton from the leaves of Psidium guajava. Chuang-Jun Li, Jie Ma, Hua Sun, Dan Zhang, and Dong-Ming Zhang* State Key Laboratory
More informationHyaluronic Acid - Conjugated Graphene Oxide / Photosensitizer. Nanohybrids for Cancer Targeted Photodynamic Therapy
Supporting Information Hyaluronic Acid - Conjugated Graphene Oxide / Photosensitizer Nanohybrids for Cancer Targeted Photodynamic Therapy Fangyuan Li, a Sin-Jung Park, b Daishun Ling b, Wooram Park b,
More informationSupporting Information
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Supporting Information A Red Emitting Mitochondrial-targeted AIE Probe as an Indicator for Membrane
More informationParameter Optimization for FEM based modeling of singlet oxygen during PDT using COMSOL
Presented at the COMSOL Conference 21 Boston COMSOL Conference, Boston, 1/8/21 Parameter Optimization for FEM based modeling of singlet oxygen during PDT using COMSOL Xing Liang, Ken Kang-Hsin Wang, and
More informationSupporting Information. Light-enhanced hypoxia-response of conjugated polymer nanocarrier. for successive synergistic photodynamic and chemo-therapy
Supporting Information Light-enhanced hypoxia-response of conjugated polymer nanocarrier for successive synergistic photodynamic and chemo-therapy Xiaolong Zhang a,d, Ming Wu a,d, Jiong Li a,c,d, Shanyou
More informationMTS assay in A549 cells
Project: VIGO MTS assay in A549 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
More informationCaspase-3 Assay Cat. No. 8228, 100 tests. Introduction
Introduction Caspase-3 Assay Cat. No. 8228, 100 tests Caspase-3 is a member of caspases that plays a key role in mediating apoptosis, or programmed cell death. Upon activation, it cleaves a variety of
More informationFree Glycerol Assay kit (Colorimetric/Fluorometric) For the rapid, sensitive and accurate measurement of Free Glycerol in various samples.
ab65337 Free Glycerol Assay kit (Colorimetric/Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of Free Glycerol in various samples. This product is for research use
More informationCalibration Protocols
Calibration Protocols (1)OD 600 Reference point LUDOX Protocol Materials: 1ml LUDOX CL-X (provided in kit) ddh20 (provided by team) 96 well plate, black with clear flat bottom preferred (provided by team)
More informationSupporting Information. for. Synthesis of dye/fluorescent functionalized. dendrons based on cyclotriphosphazene
Supporting Information for Synthesis of dye/fluorescent functionalized dendrons based on cyclotriphosphazene Aurélien Hameau 1,2, Sabine Fuchs 1,2, Régis Laurent 1,2, Jean-Pierre Majoral* 1,2 and Anne-Marie
More informationab Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric)
Version 10b Last updated 19 December 2018 ab118970 Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric) For the measurement of Lipid Peroxidation in plasma, cell culture and tissue extracts.
More informationSynergistic effects of antioxidative peptides from rice bran
Synergistic effects of antioxidative peptides from rice bran Pichamon Kiatwuthinon 1,*, Neeracha Lapanusorn 1, Anunyaporn Phungsom 1, Wirawan Tinanchai 1 1 Department of Biochemistry, Faculty of Science,
More informationConfocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex
Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is The Royal Society of Chemistry 215 Electronic Supplementary Information (ESI) Confocal and fluorescence lifetime imaging
More informationThe Energy Blocker Inside The Power House: Mitochondria. Targeted Delivery Of 3-Bromopyruvate
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2014 Supporting Information for The Energy Blocker Inside The Power House: Mitochondria Targeted
More informationSupporting Information
Supporting Information H 2 O 2 -Activatable and O 2 -Evolving Nanoparticles for Highly Efficient and Selective Photodynamic Therapy against Hypoxic Tumor Cells Huachao Chen, Jiangwei Tian, Weijiang He,*
More informationPhoton upconversion via triplet triplet annihilation
Photon upconversion via triplet triplet annihilation Photon upconversion: the process wherein light of long wavelength is frequency converted to photons of higher energy. Triplet triplet annihilation (TTA)
More informationIntracellular (Total) ROS Activity Assay Kit (Red)
Intracellular (Total) ROS Activity Assay Kit (Red) Catalog Number KA2525 200 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General
More informationDepartment of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Zhongshan II
Electronic Supplementary Material (ESI) for Medicinal Chemistry Communications. This journal is The Royal Society of Chemistry 214 Discovery of mitochondria-targeting berberine derivatives as inhibitors
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):144-149 In vitro hepatoprotective activity of ethanolic
More informationData Sheet. PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002
Data Sheet PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002 DESCRIPTION: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function
More informationAnti-Aging Skin Care Regimen. Phyto-Stem Cell: Advanced Plant Cell Culture Technology by Bio-FD&C
Anti-Aging Skin Care Regimen Phyto-Stem Cell: Advanced Plant Cell Culture Technology by Bio-FD&C 01 Plant Cell Culture Technology Highly expression of phytochemicals from plant cell culturing process by
More informationab HIV-1 Protease Inhibitor Screening Kit (Fluorometric)
Version 1 Last updated 2 March 2017 ab211106 HIV-1 Protease Inhibitor Screening Kit (Fluorometric) For the rapid, sensitive and accurate screening of potential HIV-1 Protease inhibitors. This product is
More informationInstructions. Fuse-It-Color. Overview. Specifications
Membrane fusion is a novel and highly superior method for incorporating various molecules and particles into mammalian cells. Cargo-specific liposomal carriers are able to attach and rapidly fuse with
More informationGlucose Detection Kit
ab102517 Glucose Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose levels in various samples This product is for research use only and is not intended for
More informationHahn Lab Dye Kit. Contents of dye kit:
Hahn Lab Dye Kit Contents of dye kit: dye name donor/acceptor code references (see below) Mero53 si-so-ia 4 Mero58 TD-BA-sIA - Mero59 TD-SO-sIA - Mero60 I-Pht-sIA 1 Mero61 I-BA-sIA 1 Mero62 I-TBA-sIA 1
More informationROS Activity Assay Kit
ROS Activity Assay Kit Catalog Number KA3841 200 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationDoctoral Degree Program in Marine Biotechnology, College of Marine Sciences, Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei,
Cyclooxygenase 2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents Chun-Kuang Lin 1,2, Chin-Kai Tseng 3,4, Yu-Hsuan Wu 3,4, Chih-Chuang Liaw 1,5, Chun-
More informationPorphyrin photosensitized metal organic framework for cancer cell apoptosis and caspase responsive theranostics
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information (ESI) for Chemical Communications This journal is (c) The
More informationab CytoPainter Golgi/ER Staining Kit
ab139485 CytoPainter Golgi/ER Staining Kit Instructions for Use Designed to detect Golgi bodies and endoplasmic reticulum by microscopy This product is for research use only and is not intended for diagnostic
More informationab Lipase Activity Assay Kit (Colorimetric)
ab102524 Lipase Activity Assay Kit (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of lipase activity in various samples. This product is for research use only and
More informationSupporting Information
Supporting Information Wiley-VCH 2006 69451 Weinheim, Germany Stepwise Directing Nanocrystals to Self-Assemble at Water/Oil Interfaces Jing Wang, Dayang Wang, Nelli S. Sobal, Michael Giersig, Ming Jiang,
More informationCoQ10(Coenzyme Q10) ELISA Kit
CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:
More informationAnnexin V-Cy3 Apoptosis Detection Reagent
ab14143 Annexin V-Cy3 Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples This product is for research use only and is not
More informationHigh-density Lipoprotein Cholesterol (HDL-C) Assay Kit
(FOR RESEARCH USE ONLY. DO NOT USE IT IN CLINICAL DIAGNOSIS!) High-density Lipoprotein Cholesterol (HDL-C) Assay Kit (Double reagents) Catalog No: E-BC-K221 Method: Colorimetric method Specification: 96T
More informationCinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells
Indonesian Journal of Cancer Chemoprevention, 2017, 8(1): 27-31 ISSN: 2088 0197 Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells Layung Sekar Sih Wikanthi, Nindi Wulandari,
More informationFor the rapid, sensitive and accurate measurement of Glucose in various samples
ab65333 Glucose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose in various samples This product is for research use only and is not intended for diagnostic use.
More informationPlasmonic blood glucose monitor based on enzymatic. etching of gold nanorods
Plasmonic blood glucose monitor based on enzymatic etching of gold nanorods Xin Liu, Shuya Zhang, Penglong Tan, Jiang Zhou, Yan Huang, Zhou Nie* and Shouzhuo Yao State Key Laboratory of Chemo/Biosensing
More informationGlucose Assay Kit. Catalog Number KA assays Version: 07. Intended for research use only.
Glucose Assay Kit Catalog Number KA0831 100 assays Version: 07 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
More informationAnnexin V-PE Apoptosis Detection Kit
Annexin V-PE Apoptosis Detection Kit Catalog Number KA0716 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationSensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric*
SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric* Catalog # 72146 Kit Size 500 Assays (96-well plate) Optimized Performance: This kit is optimized to detect alkaline phosphatase activity Enhanced
More informationDual aggregation-induced emission for enhanced fluorescence. sensing of furin activity in vitro and in living cells
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2016 Electronic Supplementary Information Dual aggregation-induced emission for enhanced fluorescence
More informationAnnexin V-APC/7-AAD Apoptosis Kit
Annexin V-APC/7-AAD Apoptosis Kit Catalog Number KA3808 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
More informationHuman Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General
Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General For the detection and quantitation of human OxLDL in plasma, serum or other biological fluid samples Cat. No. KT-959 For Research Use Only.
More informationThiol-Activated gem-dithiols: A New Class of Controllable. Hydrogen Sulfide (H 2 S) Donors
Thiol-Activated gem-dithiols: A New Class of Controllable Hydrogen Sulfide (H 2 S) Donors Yu Zhao, Jianming Kang, Chung-Min Park, Powell E. Bagdon, Bo Peng, and Ming Xian * Department of Chemistry, Washington
More informationSupplementary Data. Different volumes of ethanol or calcium solution were slowly added through one of four
Supplementary Data METHODS Liposome preparation Different volumes of ethanol or calcium solution were slowly added through one of four methods: Method I, no ethanol or calcium solution; Method II, exactly
More informationHuman Alpha 1 microglobulin ELISA Kit
Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:
More informationImmobilization of Samples on Slide 1.0 Hoskins Lab Last modified 03/28/2017 Chris DeCiantis
Immobilization of Samples on Slide 1. For a PEGylated slide, use 1X PBS sterile filtered buffer solution. 2. Wash the mpeg/peg-biotin using 1X PBS. Flow on 20 μl of the PBS using a 20 μl tip. The solution
More information