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1 Supplementary Figures Supplementary Fig. 1. Galectin-3 is present within tumors. (A) mrna expression levels of Lgals3 (galectin-3) and Lgals8 (galectin-8) in the four classes of cell lines as determined by gene expression microarray analysis. (B) Western blot analysis of galectin-3 and -8 expression in lysates from representative cell lines of the T nonmet (802T4), T Met (393T5), and M (393M1) classes. (C) Immunofluorescence imaging of transplanted M tumors excised from mice. Tumors were stained for macrophages (F4/80, green), galectin-3 (pink), isotype controls (IgG), and nuclei (blue). Dashed boxes in the top row highlight the area shown in the middle row. Scale bars in the top and bottom rows are 100µm. Scale bars in the middle row are 25µm
2 Supplementary Fig. 2. Analysis of peripheral blood from tumor-bearing mice by flow cytometry. (A-C) Gating strategy for isolating living single cells. (D) Gating of galectin-3 positive cells. (E) Gating of CD11b positive cells. (F) FSC vs. SSC plot depicting all cells (red) and those that are galectin-3 + (blue). Galectin-3 + cells are SSC mid to SSC hi. (G) Gating of Gr-1 + cells. (H) Gating of CD11b + Ly-6C hi and CD11b + Ly-6C lo cells. (I) CD115 (Csf1r) staining in Gr-1 - population.
3 Supplementary Fig. 3. Galectin-3 expression on CD11b + cells is not dependent on the presence of tumors. Peripheral blood from naïve and tumor-bearing mice were analyzed for galectin-3 levels on various CD11b + populations. (A) Galectin-3 geometric mean of all CD11b + cells. (B) Galectin-3 geometric mean of all Gr-1 + cells. (C) Percentage of CD11b + Gr-1 - cells that are galectin-3 positive.
4 Supplementary Fig. 4. Galectin-3 presentation on CD11b + leukocytes is not carbohydrate dependent Peripheral blood from mice bearing M flank tumors was harvested, and CD11b + leukocytes were isolated by MACS separation. Cells were incubated with β-lactose (hashed bars) or control (sucrose, solid bars) and analyzed by flow cytometry for galectin-3 expression on the various CD11b + subsets to determine whether galectin-3 presentation is carbohydrate-mediated. n.s. not significant. Significance was determined by One-way ANOVA with Tukey s Multiple Comparison Test. Error bars are s.e.m.
5 Supplementary Fig. 5. Tumor conditioned medium induces galectin-3-independent mobilization of CD11b + galectin-3 + leukocytes. Analysis of myeloid cell mobilization to peripheral blood following injections of control medium (white), M line conditioned medium (red), or recombinant murine galectin-3 supplemented control medium (gray): (A) percentage of all cells that are CD11b + ; (B) percentage of all cells that are galectin-3 + ; (C) ratio of CD11b + Ly-6C hi to CD11b + Ly-6C lo cells; (D) percentage of all cells that are CD11b + Gr-1 + ; (E) galectin-3 geometric mean of the various CD11b + subsets. P-values were calculated by One-way ANOVA with Tukey s Multiple Comparison Test. *** P < Error bars are s.e.m.
6 Supplemntary Fig. 6. Galectin-3 is not necessary for mobilization of galectin-3 + CD11b + leukocytes or metastatic seeding. (A) Concentrations of galectin-3 in media taken from M cells expressing short hairpins against a control gene (shluc, black) or galectin-3 (shgal3-3 and ahgal3-5, red) as determined by ELISA. (B-F) Analysis of myeloid cell mobilization to peripheral blood following injections of control hairpin medium (shluc, black) or shgalectin-3 medium (red): (B) percentage of all cells that are CD11b + ; (C) percentage of all cells that are galectin-3 + ; (D) percentage of all cells that are CD11b + Gr-1 + ; (E) ratio of CD11b + Ly-6C hi to CD11b + Ly-6C lo cells; (F) galectin-3 geometric mean of the various CD11b + subsets. Error bars are s.e.m. (G) Livers were harvested from mice bearing flank tumors expressing the hairpins and examined by IHC for the presence of F4/80 pos galectin-3 pos macrophages. (H) Wild-type mice were administered M cell lines containing the control (shluc) and galectin-3 hairpins by intrasplenic inoculation followed by splenectomy. Two weeks following injections, livers were harvested and imaged to visualize the presence of GFP + tumor nodules. These images are representative livers from each of the conditions. No decrease in tumor formation was visible in the shgalectin-3 lines.significance was analyzed by One-way ANOVA with Tukey s Multiple Comparison Test. n.s. not significant.
7 Supplementary Fig. 7. IL-6 induces mobilization of galectin-3 + CD11b + leukocytes. Analysis of myeloid cell mobilization to peripheral blood following injections with recombinant murine IL-6: (A) percentage of all cells that are CD11b + ; (B) percentage of all cells that are galectin-3 + ; (C) percentage of all cells that are CD11b + Gr-1 + ; (D) ratio of CD11b + Ly-6C hi to CD11b + Ly-6C lo cells. P-values were determined by Student s t-test. * P < 0.05; ** P < 0.01; *** P <
8 Supplementary Fig. 8. IL-6 induces the mobilization of galectin-3 + CD11b + granulocytes. Analysis of peripheral blood harvested from wild-type and Csf3r knockout mice following injections of recombinant IL-6 (gray) or PBS (white). (A) Percentage of all cells that are CD11b + Gr-1 + ; (B) ratio of CD11b + Ly-6C hi to CD11b + Ly-6C lo cells; (C) percentage of all cells that are CD11b + galectin-3 + ; galectin-3 geometric mean of the various CD11b + subsets. P-values were calculated by One-way ANOVA with Tukey s Multiple Comparison Test. *** P < n.s. not significant. Error bars are s.e.m.
9 Supplementary Fig. 9. Galectin-3 expression by leukocytes or stromal cells is not necessary for IL-6- dependent mobilization of myeloid cells. Analysis of peripheral blood harvested from wild-type and Lgals3 knockout mice following injections of recombinant IL-6 (pink) or PBS (white). (A) Percentage of all cells that are galectin-3 + ; (B) percentage of all cell that are CD11b + galectin-3 + ; (C) Percentage of all cells that are CD11b + Gr-1 + ; (D) ratio of CD11b + Ly-6C hi to CD11b + Ly-6C lo cells; galectin-3 geometric mean of the various CD11b + subsets. P-values were calculated by One-way ANOVA with Tukey s Multiple Comparison Test. * P < 0.05; *** P < n.s. not significant. Error bars are s.e.m.
10 Supplementary Fig. 10. The Thomsen-Friedenreich Antigen is elevated in metastatic cells and human lung cancer tissue. (A) PNA staining (green) of the clonally-related T Met and M pair of cell lines (393T5 and 393M1, respectively). Nuclei are stained with Hoechst (blue). (B) Quantification of human NSCLC tissue microarray (TMA) PNA staining in Fig. 4 by cancer subtype: LN : lymph node; Adj Lung lung tissue adjacent to (but not containing) cancerous tissue; Adeno : adenocarcinoma; LCC : large cell carcinoma; SCC : squamous cell carcinoma.
11 Supplementary Fig. 11. Gene expression microarray analysis of glycosyltransferases shows no evidence of differential regulation of transferases that generate galectin-3 ligands. (A) Gene expression microarray analysis of glycosyltransferase expression for 216 glocosyltransferases for cell lines from each of the four classes (T nonmet : blue; T Met : green; N: orange; M: red). Yellow bar: primary tumor-derived lines; red bar: metastasis-derived lines. (B) Gene expression microarray analysis of glycosyltransferase expression for transferases that generate the glycan motifs known to bind galectin-3 and -8. The predicted glycan linkages are shown to the left of the heatmap, and the heatmap depicts the expression of the transferases that generate those linkages for cell lines from each of the four classes (T nonmet : blue; T Met : green; N: orange; M: red). Yellow bar: primary tumor-derived lines; red bar: metastasis-derived lines.
12 Supplementary Fig. 12. Differential regulation of select glycosyltransferase activity prevents glycan elongation and preserves T-Antigen presentation Gene expression microarray analysis of differentiallyregulated glycosyltansferases from each of the four classes (T nonmet, T Met, and N: n=3; M: n=2). Bottom: Glycan symbol depiction of the structures resulting from the respective transferase activity. P-values in were calculated by One-way ANOVA with Tukey s Multiple Comparison Test. * P < 0.05; ** P < Error bars are s.e.m.
13 Supplementary Fig. 13. O-linked core 1 disaccharide synthesis is unaltered during metastatic progression, but branching and elongation affect T-Antigen surface presentation. (A) PNA lectin blot for whole cell lysates from the clonally-related T Met and M cell lines. (B) Gene expression microarray analysis of the human NSCLC lines shown in Fig. 4F. Cosmc (C1GALT1C1) and T-synthase (C1GALT1) promote the formation of the core 1 disaccharide. GCNT3 and GCNT1 induce branching and formation of the core 2 structure and ST6GALNAC4, ST3GAL1, and ST3GAL2 result in sialylation of the T-Antigen.
14 Supplementary Fig. 14. Transfection of Gcnt3 and knockdown of St6galnac4. (A) qrt-pcr for Gcnt3 in 393M1 cells following transfection with Gcnt3. (B) qrt-pcr for St6galnac4 following transduction with short hairpins targeting St6galnac4 or control (targeting firefly luciferase). (C) PNA staining of nonsialylated T-Antigen on 393M1 cells following transduction with short hairpins. (D) qrt-pcr for St6galnac4 following transduction with a second set of short hairpins targeting St6galnac4 or control (targeting firefly luciferase). (E) Galectin-3 binding by flow cytometry of 393M1 cells following transduction with short hairpins. Error bars are s.e.m.
15 Supplementary Fig. 15. Transfection of Gcnt3 reduces T-Antigen expression and adhesion to galectin-3. (A) Analysis of PNA labeling of the M cell line 393M1 transfected with Gcnt3 by flow cytometry for T- Antigen expression. (B) Analysis of galectin-3 binding to the M cell line 393M1 transfected with Gcnt3 by flow cytometry. P-values were determined by Student s t-test. ** P < 0.01; *** P <
16 Supplementary Fig. 16. Knockdown of St6GalNAcIV reduces the formation of metastases in vivo. Hematoxylin and eosin staining of representative liver sections from mice two weeks following intrasplenic transplantation of M cells expressing short hairpins against St6galnac4 or control (Luc, firefly luciferase). Boxed areas in the top row highlight the regions shown in the bottom row. White arrowheads denote the locations of tumor nodules in the shst6galnac4 livers. Scale bars on the top row are 2mm. Scale bars on the bottom row are 500µm.
17 Supplementary Fig. 17. Knockdown of St6galnac4 does not affect proliferation. (A) Cell counts of the M line harboring hairpins against the control gene (firefly luciferase, shluc, black) or St6galnac4 (red) were made at 24 hour intervals in cell culture.
18 Supplementary Fig. 18. Galectin-3 is elevated in mice bearing tumors. Serum galectin-3 concentrations in control mice or mice bearing bilateral T nonmet and T Met tumors as quantified by ELISA. P-value in (B) was determined by Student s t-test. **** P <
Aberrant Glycosylation Promotes Lung Cancer Metastasis through Adhesion to Galectins in the Metastatic Niche
Aberrant Glycosylation Promotes Lung Cancer Metastasis through Adhesion to Galectins in the Metastatic Niche The MIT Faculty has made this article openly available. Please share how this access benefits
Supplemental Table S1
Supplemental Table S. Tumorigenicity and metastatic potential of 44SQ cell subpopulations a Tumorigenicity b Average tumor volume (mm ) c Lung metastasis d CD high /4 8. 8/ CD low /4 6./ a Mice were injected
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doi:1.138/nature1554 a TNF-α + in CD4 + cells [%] 1 GF SPF 6 b IL-1 + in CD4 + cells [%] 5 4 3 2 1 Supplementary Figure 1. Effect of microbiota on cytokine profiles of T cells in GALT. Frequencies of TNF-α
Supplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
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Supplementary Information Häuselmann et al. Monocyte induction of E-selectin-mediated endothelial activation releases VE-cadherin junctions to promote tumor cell extravasation in the metastasis cascade
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A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
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Supplement Material. Spleen weight (mg) LN cells (X106) Acat1-/- Acat1-/- Mouse weight (g)
Supplement Material A Spleen weight (mg) C Mouse weight (g) 1 5 1 2 9 6 3 2 5 2 1 5 Male LN cells (X16) 4 ** ** Female B 3 2 1 Supplemental Figure I. Spleen weight (A), Inguinal lymph node (LN) cell number
Supplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
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Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic B cells from WT Nfat2 +/+, TCL1 Nfat2 +/+ and TCL1 Nfat2
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Supplementary Figure 1 Chemokine and chemokine receptor expression during muscle regeneration (a) Analysis of CR3CR1 mrna expression by real time-pcr
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SUPPLEMENTARY FIGURE 1 A LN Cell count (1 ) 1 3 1 CD+ 1 1 CDL lo CD hi 1 CD+FoxP3+ 1 1 1 7 3 3 3 % of cells 9 7 7 % of cells CD+ 3 1 % of cells CDL lo CD hi 1 1 % of CD+ cells CD+FoxP3+ 3 1 % of CD+ T
Bezzi et al., Supplementary Figure 1 *** Nature Medicine: doi: /nm Pten pc-/- ;Zbtb7a pc-/- Pten pc-/- ;Pml pc-/- Pten pc-/- ;Trp53 pc-/-
Gr-1 Gr-1 Gr-1 Bezzi et al., Supplementary Figure 1 a Gr1-CD11b 3 months Spleen T cells 3 months Spleen B cells 3 months Spleen Macrophages 3 months Spleen 15 4 8 6 c CD11b+/Gr1+ cells [%] 1 5 b T cells
Supplemental Material
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Nature Medicine: doi: /nm.3922
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Supplementary Figures Supplementary Figure 1 DOT1L regulates the expression of epithelial and mesenchymal markers. (a) The expression levels and cellular localizations of EMT markers were confirmed by
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1 Supplemental Figure 1 Effects of DATE shortening on HGF promoter activity. The HGF promoter region (-1037 to +56) containing wild-type (30As) or truncated DATE (26As, 27As, 28A, 29As) from breast cancer
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Type of file: PDF Size of file: 0 KB Title of file for HTML: Supplementary Information Description: Supplementary Figures
Type of file: PDF Size of file: 0 KB Title of file for HTML: Supplementary Information Description: Supplementary Figures Supplementary Figure 1 mir-128-3p is highly expressed in chemoresistant, metastatic
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doi:10.1038/nature10134 Supplementary Figure 1. Anti-inflammatory activity of sfc. a, Autoantibody immune complexes crosslink activating Fc receptors, promoting activation of macrophages, and WWW.NATURE.COM/NATURE
Supplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
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Fig S1 A B Tumors per mouse 1.2 1.0 0.8 0.6 0.4 0.2 0.0 All T-lymph B-lymph Sarcomas Carcinomas Germ cell Tumor type -/- (n = 46) Q/- (n = 76) Q/Q (n = 31) Tumors per mouse 1.2 1.0 0.8 0.6 0.4 0.2 0.0
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Pathologic Stage. Lymph node Stage
ASC ASC a c Patient ID BMI Age Gleason score Non-obese PBMC 1 22.1 81 6 (3+3) PBMC 2 21.9 6 6 (3+3) PBMC 3 22 84 8 (4+4) PBMC 4 24.6 68 7 (3+4) PBMC 24. 6 (3+3) PBMC 6 24.7 73 7 (3+4) PBMC 7 23. 67 7 (3+4)
Supplementary Materials for
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Supporting Information
Supporting Information M1 macrophage-derived nanovesicles potentiate the anticancer efficacy of immune checkpoint inhibitors Yeon Woong Choo, 1, Mikyung Kang, 2, Han Young Kim, 1 Jin Han, 1 Seokyung Kang,
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Supplementary information a +KA Relative expression d! Tlr9 5!! 5! NSC Neuron Astrocyte Microglia! 5! Tlr7!!!! NSC Neuron Astrocyte! GFP/Sβ/! Iba/Hoechst Microglia e Hoechst/Iba/TLR9! GFP/Iba/GFAP f Brain
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DOI:.38/ncb3399 a b c d FSP DAPI 5mm mm 5mm 5mm e Correspond to melanoma in-situ Figure a DCT FSP- f MITF mm mm MlanaA melanoma in-situ DCT 5mm FSP- mm mm mm mm mm g melanoma in-situ MITF MlanaA mm mm
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Supplementary Information Title Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis Authors Shin H. Kang, Ying Li, Masahiro Fukaya, Ileana Lorenzini,
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Supplementary Table 1
Supplementary Table 1 Flow Cytometry Antibodies Antibody Fluorochrome Clone Vendor CD45 PE-cyanine 7 30-F11 D ioscience CD3 Pacific lue 17A2 iolegend (San Diego, CA) CD11b APC M1/70 iolegend (San Diego,
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Nature Immunology: doi: /ni Supplementary Figure 1. RNA-Seq analysis of CD8 + TILs and N-TILs.
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Nature Immunology: doi: /ni Supplementary Figure 1. Id2 and Id3 define polyclonal T H 1 and T FH cell subsets.
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Supplemental information
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File Name: Supplementary Information Description: Supplementary Figures and Supplementary Table File Name: Peer Review File Description: Supplementary Table 1 Primers and taqman probes used were the following:
Supplementary Figure 1. Validation of astrocytes. Primary astrocytes were
Supplementary Figure 1. Validation of astrocytes. Primary astrocytes were separated from the glial cultures using a mild trypsinization protocol. Anti-glial fibrillary acidic protein (GFAP) immunofluorescent
SUPPLEMENTARY INFORMATION
1. Supplementary Figures and Legends Supplementary Fig. 1. S1P-mediated transcriptional regulation of integrins expressed in OP/monocytoid cells. Real-time quantitative PCR analyses of mrna for two integrins,
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Díaz et al., http://www.jcb.org/cgi/content/full/jcb.201209151/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Hypoxia induces invadopodia formation in different epithelial
Supplementary Figure 1. Identification of tumorous sphere-forming CSCs and CAF feeder cells. The LEAP (Laser-Enabled Analysis and Processing)
Supplementary Figure 1. Identification of tumorous sphere-forming CSCs and CAF feeder cells. The LEAP (Laser-Enabled Analysis and Processing) platform with laser manipulation to efficiently purify lung
Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
SUPPLEMENTARY INFORMATION Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation Samantha Arokiasamy 1,2, Christian Zakian 1, Jessica Dilliway
Nature Immunology: doi: /ni Supplementary Figure 1. Huwe1 has high expression in HSCs and is necessary for quiescence.
Supplementary Figure 1 Huwe1 has high expression in HSCs and is necessary for quiescence. (a) Heat map visualizing expression of genes with a known function in ubiquitin-mediated proteolysis (KEGG: Ubiquitin
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Supplemental Figure 1. Furin is efficiently deleted in CD4 + and CD8 + T cells. a, Western blot for furin and actin proteins in CD4cre-fur f/f and fur f/f Th1 cells. Wild-type and furin-deficient CD4 +
Supplementary Information. Tissue-wide immunity against Leishmania. through collective production of nitric oxide
Supplementary Information Tissue-wide immunity against Leishmania through collective production of nitric oxide Romain Olekhnovitch, Bernhard Ryffel, Andreas J. Müller and Philippe Bousso Supplementary
TEB. Id4 p63 DAPI Merge. Id4 CK8 DAPI Merge
a Duct TEB b Id4 p63 DAPI Merge Id4 CK8 DAPI Merge c d e Supplementary Figure 1. Identification of Id4-positive MECs and characterization of the Comma-D model. (a) IHC analysis of ID4 expression in the
Supplementary Figure 1.
Supplementary Figure 1. Female Pro-ins2 -/- mice at 5-6 weeks of age were either inoculated i.p. with a single dose of CVB4 (1x10 5 PFU/mouse) or PBS and treated with αgalcer or control vehicle. On day
SUPPLEMENTARY INFORMATION
doi:10.1038/nature12652 Supplementary Figure 1. PRDM16 interacts with endogenous EHMT1 in brown adipocytes. Immunoprecipitation of PRDM16 complex by flag antibody (M2) followed by Western blot analysis
days days and gbt-i.cd Recipient 20
gbt-i. GFP+ Resident memory cells: gbt-i.gfp+ Recruited memory cells: gbt-i.cd45.1+ 1 2-3 gbt-i. flu.gb sc. CD45.1+ Graft with gbt-i.gfp+ 1 Recipient 1 re- 3 36 Graft with gbt-i.gfp+ and gbt-i.cd45.1+
Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures
Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures Type of file: MOV Title of file for HTML: Supplementary Movie 1 Description: NLRP3 is moving along
Supplementary Figure 1
Supplementary Figure 1 Genetic labeling of microglia Male and female 2-3 month-old CreERT2;R26-tdTomato mice or CreERT2;R26-tdTomato;Iba1-eGFP transgenic mice were treated with 1x, 2x (48 h apart), or
Supplementary Figure 1. Nature Neuroscience: doi: /nn.4547
Supplementary Figure 1 Characterization of the Microfetti mouse model. (a) Gating strategy for 8-color flow analysis of peripheral Ly-6C + monocytes from Microfetti mice 5-7 days after TAM treatment. Living
Supplementary Figure 1. Supernatants electrophoresis from CD14+ and dendritic cells. Supernatants were resolved by SDS-PAGE and stained with
Supplementary Figure 1. Supernatants electrophoresis from CD14+ and dendritic cells. Supernatants were resolved by SDS-PAGE and stained with Coomassie brilliant blue. One µg/ml recombinant human (rh) apo-e
University of Miami Miller School of Medicine, Miami, FL 33136, USA, 3 State Key Laboratory
Supplementary File ASXL1 plays an important role in erythropoiesis Hui Shi 1,2,3, Shohei Yamamoto 1,2,4, Mengyao Sheng 3, Jie Bai 3, Peng Zhang 1,2, Runze Chen 1,2, Shi Chen 1,2, Lihong Shi 3, Omar Abdel-Wahab
a surface permeabilized
a surface permeabilized RAW 64.7 P388D1 J774 b CD11b + Ly-6G - Blood Monocytes WT Supplementary Figure 1. Cell surface expression on macrophages and DCs. (a) RAW64.7, P388D1, and J774 cells were subjected
CD4 + T cells recovered in Rag2 / recipient ( 10 5 ) Heart Lung Pancreas
a CD4 + T cells recovered in Rag2 / recipient ( 1 5 ) Heart Lung Pancreas.5 1 2 4 6 2 4 6 Ctla4 +/+ Ctla4 / Ctla4 / Lung Ctla4 / Pancreas b Heart Lung Pancreas Ctla4 +/+ Ctla4 / Ctla4 / Lung Ctla4 / Pancreas
Supplementary Figure 1. Generation of knockin mice expressing L-selectinN138G. (a) Schematics of the Sellg allele (top), the targeting vector, the
Supplementary Figure 1. Generation of knockin mice expressing L-selectinN138G. (a) Schematics of the Sellg allele (top), the targeting vector, the targeted allele in ES cells, and the mutant allele in
Supplementary Figure S I: Effects of D4F on body weight and serum lipids in apoe -/- mice.
Supplementary Figures: Supplementary Figure S I: Effects of D4F on body weight and serum lipids in apoe -/- mice. Male apoe -/- mice were fed a high-fat diet for 8 weeks, and given PBS (model group) or
Breeding scheme, transgenes, histological analysis and site distribution of SB-mutagenized osteosarcoma.
Supplementary Figure 1 Breeding scheme, transgenes, histological analysis and site distribution of SB-mutagenized osteosarcoma. (a) Breeding scheme. R26-LSL-SB11 homozygous mice were bred to Trp53 LSL-R270H/+
SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28
Supp. Fig. 1 a APC b APC ICOS ICOS TCR CD28 mir P TCR CD28 P T cell Tolerance Roquin WT SG Icos mrna T cell Autoimmunity Roquin M199R SG Icos mrna www.nature.com/nature 1 Supp. Fig. 2 CD4 + CD44 low CD4
Supplementary Figure 1. A. Bar graph representing the expression levels of the 19 indicated genes in the microarrays analyses comparing human lung
Supplementary Figure 1. A. Bar graph representing the expression levels of the 19 indicated genes in the microarrays analyses comparing human lung immortalized broncho-epithelial cells (AALE cells) expressing
supplementary information
DOI: 10.1038/ncb2133 Figure S1 Actomyosin organisation in human squamous cell carcinoma. (a) Three examples of actomyosin organisation around the edges of squamous cell carcinoma biopsies are shown. Myosin
Supplementary information. The proton-sensing G protein-coupled receptor T-cell death-associated gene 8
1 Supplementary information 2 3 The proton-sensing G protein-coupled receptor T-cell death-associated gene 8 4 (TDAG8) shows cardioprotective effects against myocardial infarction 5 Akiomi Nagasaka 1+,
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DOI: 10.1038/ncb2607 Figure S1 Elf5 loss promotes EMT in mammary epithelium while Elf5 overexpression inhibits TGFβ induced EMT. (a, c) Different confocal slices through the Z stack image. (b, d) 3D rendering
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured under Th0, Th1, Th2, Th17, and Treg conditions. mrna
SUPPLEMENTARY FIGURES AND TABLE
SUPPLEMENTARY FIGURES AND TABLE Supplementary Figure S1: Characterization of IRE1α mutants. A. U87-LUC cells were transduced with the lentiviral vector containing the GFP sequence (U87-LUC Tet-ON GFP).
Supplementary Table 1. List of primers used in this study
Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3
Nature Immunology: doi: /ni Supplementary Figure 1. Cellularity of leukocytes and their progenitors in naive wild-type and Spp1 / mice.
Supplementary Figure 1 Cellularity of leukocytes and their progenitors in naive wild-type and Spp1 / mice. (a, b) Gating strategies for differentiated cells including PMN (CD11b + Ly6G hi and CD11b + Ly6G
Supplementary Figure 1. Successful excision of genes from WBM lysates and
Supplementary Information: Supplementary Figure 1. Successful excision of genes from WBM lysates and survival of mice with different genotypes. (a) The proper excision of Pten, p110α, p110α and p110δ was