Supplementary Figures
|
|
|
- Stephany Sanders
- 5 years ago
- Views:
Transcription
1 Supplementary Figures Supplementary Figure 1 Correlation between LKB1 and YAP expression in human lung cancer samples. (a) Representative photos showing LKB1 and YAP immunohistochemical staining in human lung cancer. Scale bar, 150 m. (b) Correlation analysis between LKB1 and YAP expression (P=0.511) in human lung cancer. 2-test was used to determine the correlation between LKB1 and YAP expression.
2 Supplementary Figure 2 TAZ expression in human lung ADC and SCC. (a) Representative photos showing TAZ immunohistochemical staining in human lung ADC and SCC. Scale bar, 150 m. (b) Statistical analysis of TAZ expression in human lung ADC and SCC. 2-test was used to determine the expression difference.
3 Supplementary Figure 3 YAP over-expression validation in KL and YKL mice. (a-b) Realtime PCR quantification (a) and western blot (b) of YAP in lung tumors from KL mice delivered with Lenti-CMV-Cre or Lenti-YAPS127A-Cre (each sample was assayed in triplicates; Representative data of western blot were shown from three independent experiments.). (c) Realtime PCR quantification of YAP in lung tissue from WT and SP-C-YAP transgenic mice (each sample was assayed in triplicates). (d) Western blot of YAP in lung tumors from KL or YKL mice from three SP-C-YAP transgenic lines. Representative data were shown from three independent experiments.
4 Supplementary Figure 4 Yap knockdown decreases the cell proliferation in lung ADC from KL mouse model. (a) Western blot of Yap in lung tumors from KL mice delivered with Lenti-U6-Cre or Lenti-shYap-Cre. Representative data were shown from three independent experiments. (b-c) Ki-67 and Cleaved Caspase-3 immunohistochemical staining in lung tumors from KL mice delivered with Lenti-U6-Cre or Lenti-shYap-Cre. Scale bar, 150 μm. Data are shown as mean±sem. t-test, ***, P<0.005.
5 Supplementary Figure 5 YAP knockdown impairs cell proliferation and anchorage independent cell growth in A549 cells. (a) Western blot validation of efficient Yap knockdown in A549 cells. Representative data were shown from three independent experiments. (b) MTT assay showing that YAP knockdown in A549 cells significantly reduced cell proliferation (each sample was assayed in triplicates). (c) Colony formation assay showing that YAP knockdown in A549 cells significantly impaired anchorage independent cell growth in soft agar (each sample was assayed in triplicates). Representative data of MTT assay and colony formation assay were shown from three independent experiments. Data are shown as mean±sem. t-test, ***, P<0.005.
6 Supplementary Figure 6 YAP negatively regulates DNp63 transcription in human lung cancer cell lines. (a) Ectopic YAP S127A expression down-regulated DNp63 mrna and protein levels in SH-416 cells. Representative data of western blot were shown from three independent experiments. (b)yap S127A over-expression down-regulated DNp63 mrna levels in A549, CRL-5908, CRL-5807 and CRL-5810 cells. (c) Knockdown of YAP up-regulated DNp63 mrna in A549 cells. (d) Ectopic expression of YAP 5SA but not YAP 5SA with C-terminal trans-activation domain deletion dramatically decreased DNp63 mrna levels in A549 cells. The asterisk represents the YAP 5SA-ΔC mutant form with its C-terminal trans-activation domain deletion. Representative data of western blot were shown from three independent experiments. (e) Knockdown of TEADs reversed the decrease of DNp63 mrna and protein levels caused by YAP S127A ectopic expression in CRL-1848 cells. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR and western blot were shown from three independent experiments. Data are shown as mean±sem. t-test, *, P<0.05; **, P<0.01; ***, P<0.005.
7 Supplementary Figure 7 Extracellular matrix (ECM) deprivation leads to YAP phosphorylation and inactivation. (a) Realtime PCR quantification of Yap target gene transcripts in ADC and SCC from KL mice (each sample was assayed in triplicates by quantitative real-time PCR).Fold change was calculated as log2 (SCC/ADC). (b) Representative photos showing YAP immunohistochemical staining in KL mice treated with BAPN or saline. Scale bar, 150 μm. (c) Cell detachment from ECM resulted in YAP phosphorylation and inactivation in A549 cells. A549 cells were either trypsinized (T), or trypsinized and then attached onto collagen I-coated petri dishes for 2 h (A), or trypsinized and then cultured on Poly-HEMA coated dishes in suspension for 2h (S), or further trypsinized after attachment (ret). Samples were lysed and analyzed by Western blots. Representative data of western blot were shown from three independent experiments.
8 Supplementary Figure 8 Screen for YAP downstream repressors regulating DNp63 transcription. (a-b) Realtime PCR analysis of relative expression of 5 common E-box binding factors including SNAIL, SLUG, ZEB1, ZEB2 and MYC following YAP S127A ectopic expression in A549 (a) and HTB-182 (b) cells. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR were shown from three independent experiments. Data are shown as mean±sem. t-test, *, P<0.05; **, P<0.01.
9 Supplementary Figure 9 YAP up-regulates ZEB2 transcription in human lung cancer cell lines. (a-b) YAP S127A ectopic expression up-regulated ZEB2 mrna and protein levels in A549 (a) and CRL-1848 (b) cells. (c-d) Knockdown of YAP down-regulated ZEB2 mrna and protein levels in A549 (c) and CRL-1848 (d) cells. (e-f) Ectopic expression of YAP 5SA but not YAP 5SA with C-terminal transactivation domain deletion dramatically increased ZEB2 mrna and protein levels in A549 (e) and CRL-1848 (f) cells. The asterisk represents the YAP 5SA-ΔC mutant form with its c-terminal trans-activation domain deletion. (g) Knockdown of TEADs blocked the increase of ZEB2 mrna levels caused by YAP S127A ectopic expression in A549 cells. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR and western blot were shown from three independent experiments. Data are shown as mean±sem. t-test, *, P<0.05; **, P<0.01; ***, P<0.005.
10 Supplementary Figure 10 ZEB2 represses DNp63 transcription in human lung cancer cell lines. (a-b) ZEB2 ectopic expression down-regulated DNp63 mrna and protein levels in A549 (a) and CRL-1848 (b) cells. (c-d) Knockdown of ZEB2 up-regulated DNp63 mrna and protein levels in A549 (c) and CRL-1848 (d) cells. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR and western blot were shown from three independent experiments. Data are shown as mean±sem. t-test, *, P<0.05; **, P<0.01.
11 Supplementary Figure 11 YAP suppresses DNp63 expression in a ZEB2 dependent manner. (a-b) Knockdown of ZEB2 reversed the decrease of DNp63 mrna and protein levels caused by YAP ectopic expression in A549 (a) and CRL-1848 (b) cells. (c-d) Re-introduction of ZEB2 abolished the increase of DNp63 mrna and protein levels resulted from YAP knockdown in A549 (c) and CRL-1848 (d) cells. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR and western blot were shown from three independent experiments. Data are shown as mean±sem. t-test, **, P<0.01; ***, P<0.005.
12 Supplementary Figure 12 Estrogen receptor (ER)-mediated DNp63 inducible expression validation in vitro. Western blot using cytoplasmic and nuclear fractions showed that TAM treatment increased nuclear DNp63 expression with a concomitant decrease of cytoplasmic DNp63 level. LAMIN B and TUBLIN served as loading controls for nuclear and cytoplasmic fractions, respectively. Representative data of western blot were shown from three independent experiments.
13 Supplementary Figure 13 Original photos of western blots and gels shown in the main text.
14 Supplementary Figure 14 Original photos of western blots shown in Supplementary Information.
15 Supplementary Table 1. Clinical correlation between YAP expression and key oncogenic drivers in human NSCLC. YAP Characteristics Low High P EGFR Mut. Negative 86 (74.8%) 29 (25.2%) <0.001*** Positive 22 (43.1%) 29 (56.9%) KRAS Mut. Negative 103 (64.8%) 56 (35.2%) Positive 5 (71.4%) 2 (28.6%) HER2 Mut Negative 105 (64.8%) 57 (35.2%) Positive 3 (75.0%) 1 (25.0%) BRAF Mut Negative 105 (64.8%) 57 (35.2%) Positive 3 (75.0%) 1 (25.0%) EML4-ALK fusions Negative 100 (63.3%) 58 (36.7%) Positive 8 (100%) 0 (0%) FGFR fusions Negative 104 (64.6%) 57 (35.4%) Positive 4 (80%) 1 (20%) 2-test and Fisher s Exact Test (n<5) were used to generate the P value.
16 Supplementary Table 2. Clinical correlation between YAP expression and key oncogenic drivers in human lung ADC. YAP Characteristics Low High P EGFR Mut. Negative 26 (74.3%) 9 (25.7%) 0.004** Positive 20 (42.6%) 27 (57.4%) KRAS Mut. Negative 44 (55.7%) 35 (44.3%) Positive 2 (66.7%) 1 (33.3%) HER2 Mut Negative 43 (55.1%) 35 (44.9%) Positive 3 (75.0%) 1 (25.0%) BRAF Mut Negative 44 (55.7%) 35 (44.3%) Positive 2 (66.7%) 1 (33.3%) EML4-ALK fusions Negative 38 (51.4%) 36 (48.6%) 0.008** Positive 8 (100%) 0 (0%) 2-test and Fisher s Exact Test (n<5) were used to generate the P value.
17 Supplementary Table 3. Clinical correlation between YAP expression and FGFR fusions in human lung SCC. YAP Characteristics Low High P FGFR fusions Negative 58 (73.4%) 21 (26.6%) Positive 4 (80.0%) 1 (20.0%) Fisher s Exact Test (n<5) was used to generate the P value.
18 Supplementary Table 4. Correlation analysis between Zeb2 and p63 expression in lung tumors from KL mice. Zeb2 Low High P Low <0.001*** p % 80.4% High % 30.4% 2-test was used to generate the P value.
19 Supplementary Table 5. The list of primers used in this study. Gene Forward primer Reverse primer p63 GGAAAACAATGCCCAGACTC GTGGAATACGTCCAGGTGGC CTGF CCTGGTCCAGACCACAGAGT TGGAGATTTTGGGAGTACGG Ctgf AGCAGCTGGGAGAACTGTGT GCTGCTTTGGAAGGACTCAC CYR61 ACCGCTCTGAAGGGGATCT ACTGATGTTTACAGTTGGGCTG Cyr61 GATGACCTCCTCGGACTCGAT CGTGCAGAGGGTTGAAAAGAA ITGB2 TGCGTCCTCTCTCAGGAGTG GGTCCATGATGTCGTCAGCC Itgb2 CAGGAATGCACCAAGTACAAAGT GTCACAGCGCAAGGAGTCA GLI2 CCCCTACCGATTGACATGCG GAAAGCCGGATCAAGGAGATG Gli2 CAACGCCTACTCTCCCAGAC GAGCCTTGATGTACTGTACCAC FGF1 ATACGGCTCACAGACACCAA CGTTTGCAGCTCCCATTCTT Fgf1 CTCGCAGACACCAAATGAGG ACAGCTCCCGTTCTTCTTGA AREG GAGCCGACTATGACTACTCAGA TCACTTTCCGTCTTGTTTTGGG Areg GGGGACTACGACTACTCAGAG TCTTGGGCTTAATCACCTGTTC AXL CCGTGGACCTACTCTGGCT CCTTGGCGTTATGGGCTTC Axl ATGGCCGACATTGCCAGTG CGGTAGTAATCCCCGTTGTAGA YAP CACAGCATGTTCGAGCTCAT GATGCTGAGCTGTGGGTGTA GAPDH CAGGTGGTCTCCTCTGACTT CCAAATTCGTTGTCATACCA β-actin TGAGCGCAAGTACTCTGTGTGGAT ACTCATCGTACTCCTGCTTGCTGA SNAIL CTGCCCTGCGTCTGCGGAAC GGAGCGGTCAGCGAAGGCAC SLUG CCGCGCTCCTTCCTGGTCAA TGGAGCAGCGGTAGTCCACACA ZEB1 AGCAGTGAAAGAGAAGGGAATGC GGTCCTCTTCAGGTGCCTCAG ZEB2 CAAGAGGCGCAAACAAGCC GGTTGGCAATACCGTCATCC MYC TCAAGAGGCGAACACACAAC GGCCTTTTCATTGTTTTCCA Col1a1 GCTCCTCTTAGGGGCCACT CCACGTCTCACCATTGGGG Col3a1 CTGTAACATGGAAACTGGGGAAA CCATAGCTGAACTGAAAACCACC Col4a1 CTGGCACAAAAGGGACGAG ACGTGGCCGAGAATTTCACC Col4a2 GACCGAGTGCGGTTCAAAG CGCAGGGCACATCCAACTT Col4a3 CAAAGGCATCAGGGGAATAACT ATCCGTTGCATCCTGGTAAAC Col4a5 GGAGAACGGGGGTTTCCAG CTCCCTTGGTTCCATTGCATC Col5a2 ACAGGTGAAGTGGGATTCTCA CCATAGCACCCATTGGACCA Col6a1 CTGCTGCTACAAGCCTGCT CCCCATAAGGTTTCAGCCTCA Col7a1 GCCCAGAGATAGAGTGACCTG CGCACTTCTCGAAAGTTGCTG Col18a1 GTGCCCATCGTCAACCTGAA GACATCTCTGCCGTCAAAAGAA Timp1 GCAACTCGGACCTGGTCATAA CGGCCCGTGATGAGAAACT Timp2 TCAGAGCCAAAGCAGTGAGC GCCGTGTAGATAAACTCGATGTC Timp3 CTTCTGCAACTCCGACATCGT GGGGCATCTTACTGAAGCCTC Timp4 TGTGGCTGCCAAATCACCA TCATGCAGACATAGTGCTGGG Mmp9 TGTCTGGAGATTCGACTTGAAGTC TGGTGTGCCCTGGAACTCA Mmp14 CAGTATGGCTACCTACCTCCAG GCCTTGCCTGTCACTTGTAAA Lox TCTTCTGCTGCGTGACAACC GAGAAACCAGCTTGGAACCAG Loxl1 TGCCCGACAACTGGAGAGA TGCGGATAGGGGAACTGCT
20 Loxl2 ATTAACCCCAACTATGAAGTGCC CTGTCTCCTCACTGAAGGCTC GAPDH promoter ChIP CCTTCTTGCCTTGCTCTTGCTAC GCCTGCCTGGTGATAATCTTTG ZEB2 promoter ChIP AATATCATGCTCTCCCTTCC TGGCATTTTAATAAAGGGTC DNp63 promoter ChIP TCCATTGGAGTGGAGGAGTC GCTGCTGTCCTTTCTTCTGG
Supplementary Figure 1. Lkb1-deficient lung ADC progressively transdifferentiates into SCC. (a) A scheme showing the progression pattern of atypical
Supplementary Figure 1. Lkb1-deficient lung ADC progressively transdifferentiates into SCC. (a) A scheme showing the progression pattern of atypical adenomatous hyperplasia/epithelial hyperplasia (AAH/EH),
Supplementary Figures
Supplementary Figures Supplementary Figure 1 DOT1L regulates the expression of epithelial and mesenchymal markers. (a) The expression levels and cellular localizations of EMT markers were confirmed by
Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the
Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the genome-wide methylation microarray data. Mean ± s.d.; Student
(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a
Supplementary figure legends Supplementary Figure 1. Expression of Shh signaling components in a panel of gastric cancer. (A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and
(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)
Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory
Nature Medicine: doi: /nm.4324
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Supplementary Figure 1. Kinetics of SnCs development in surgically-induced OA and effect of GCV-induced SnC clearance on OA disease progression
Supplementary Figure 1
Combination index (CI) Supplementary Figure 1 2. 1.5 1. Ishikawa AN3CA Nou-1 Hec-18.5...2.4.6.8 1. Fraction affected (Fa) Supplementary Figure 1. The synergistic effect of PARP inhibitor and PI3K inhibitor
supplementary information
DOI: 10.1038/ncb1875 Figure S1 (a) The 79 surgical specimens from NSCLC patients were analysed by immunohistochemistry with an anti-p53 antibody and control serum (data not shown). The normal bronchi served
Figure S1. Sorting nexin 9 (SNX9) specifically binds psmad3 and not psmad 1/5/8. Lysates from AKR-2B cells untreated (-) or stimulated (+) for 45 min
Figure S1. Sorting nexin 9 (SNX9) specifically binds psmad3 and not psmad 1/5/8. Lysates from AKR2B cells untreated () or stimulated () for 45 min with 5 ng/ml TGFβ or 10 ng/ml BMP4 were incubated with
Supplementary Information and Figure legends
Supplementary Information and Figure legends Table S1. Primers for quantitative RT-PCR Target Sequence (5 -> 3 ) Target Sequence (5 -> 3 ) DAB2IP F:TGGACGATGTGCTCTATGCC R:GGATGGTGATGGTTTGGTAG Snail F:CCTCCCTGTCAGATGAGGAC
SUPPLEMENTARY FIGURES AND TABLE
SUPPLEMENTARY FIGURES AND TABLE Supplementary Figure S1: Characterization of IRE1α mutants. A. U87-LUC cells were transduced with the lentiviral vector containing the GFP sequence (U87-LUC Tet-ON GFP).
Supplementary methods:
Supplementary methods: Primers sequences used in real-time PCR analyses: β-actin F: GACCTCTATGCCAACACAGT β-actin [11] R: AGTACTTGCGCTCAGGAGGA MMP13 F: TTCTGGTCTTCTGGCACACGCTTT MMP13 R: CCAAGCTCATGGGCAGCAACAATA
Supplemental Figure 1. Western blot analysis indicated that MIF was detected in the fractions of
Supplemental Figure Legends Supplemental Figure 1. Western blot analysis indicated that was detected in the fractions of plasma membrane and cytosol but not in nuclear fraction isolated from Pkd1 null
Supplementary Table 1. List of primers used in this study
Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3
Supplementary Figure 1.TRIM33 binds β-catenin in the nucleus. a & b, Co-IP of endogenous TRIM33 with β-catenin in HT-29 cells (a) and HEK 293T cells
Supplementary Figure 1.TRIM33 binds β-catenin in the nucleus. a & b, Co-IP of endogenous TRIM33 with β-catenin in HT-29 cells (a) and HEK 293T cells (b). TRIM33 was immunoprecipitated, and the amount of
Supplementary Figure 1
Supplementary Figure 1 YAP negatively regulates IFN- signaling. (a) Immunoblot analysis of Yap knockdown efficiency with sh-yap (#1 to #4 independent constructs) in Raw264.7 cells. (b) IFN- -Luc and PRDs
Supplementary Figure 1. A. Bar graph representing the expression levels of the 19 indicated genes in the microarrays analyses comparing human lung
Supplementary Figure 1. A. Bar graph representing the expression levels of the 19 indicated genes in the microarrays analyses comparing human lung immortalized broncho-epithelial cells (AALE cells) expressing
Problem Set 8 Key 1 of 8
7.06 2003 Problem Set 8 Key 1 of 8 7.06 2003 Problem Set 8 Key 1. As a bright MD/PhD, you are interested in questions about the control of cell number in the body. Recently, you've seen three patients
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2607 Figure S1 Elf5 loss promotes EMT in mammary epithelium while Elf5 overexpression inhibits TGFβ induced EMT. (a, c) Different confocal slices through the Z stack image. (b, d) 3D rendering
Supplementary Figure 1. The CagA-dependent wound healing or transwell migration of gastric cancer cell. AGS cells transfected with vector control or
Supplementary Figure 1. The CagA-dependent wound healing or transwell migration of gastric cancer cell. AGS cells transfected with vector control or 3xflag-CagA expression vector were wounded using a pipette
Plasma exposure levels from individual mice 4 hours post IP administration at the
Supplemental Figure Legends Figure S1. Plasma exposure levels of MKC-3946 in mice. Plasma exposure levels from individual mice 4 hours post IP administration at the indicated dose mg/kg. Data represent
Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model.
A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
SUPPLEMENTARY INFORMATION
doi:10.1038/nature12652 Supplementary Figure 1. PRDM16 interacts with endogenous EHMT1 in brown adipocytes. Immunoprecipitation of PRDM16 complex by flag antibody (M2) followed by Western blot analysis
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:1.138/nature9814 a A SHARPIN FL B SHARPIN ΔNZF C SHARPIN T38L, F39V b His-SHARPIN FL -1xUb -2xUb -4xUb α-his c Linear 4xUb -SHARPIN FL -SHARPIN TF_LV -SHARPINΔNZF -SHARPIN
EPIGENETIC RE-EXPRESSION OF HIF-2α SUPPRESSES SOFT TISSUE SARCOMA GROWTH
EPIGENETIC RE-EXPRESSION OF HIF-2α SUPPRESSES SOFT TISSUE SARCOMA GROWTH Supplementary Figure 1. Supplementary Figure 1. Characterization of KP and KPH2 autochthonous UPS tumors. a) Genotyping of KPH2
Supplementary Figure 1 ITGB1 and ITGA11 increase with evidence for heterodimers following HSC activation. (a) Time course of rat HSC activation
Supplementary Figure 1 ITGB1 and ITGA11 increase with evidence for heterodimers following HSC activation. (a) Time course of rat HSC activation indicated by the detection of -SMA and COL1 (log scale).
Supplementary Information
Supplementary Information TABLE S1. SUBJECT CHARACTERISTICS* Normal Control Subjects Subjects with Asthma p Value Number 23 48 Age (years) 35±10 35±10 0.75 Sex, M:F (% F) 9:12 (57) 17:26 (60) 0.76 FEV1
Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients.
Supplementary Materials Supplementary Figures Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients. Figure S2. Expression level of podocyte
Supplementary Figure 1. Basal level EGFR across a panel of ESCC lines. Immunoblots demonstrate the expression of phosphorylated and total EGFR as
Supplementary Figure 1. Basal level EGFR across a panel of ESCC lines. Immunoblots demonstrate the expression of phosphorylated and total EGFR as well as their downstream effectors across a panel of ESCC
SOPten flox/flox (KO) Pten flox/flox (WT) flox allele 6.0 kb. Pten. Actin. ! allele 2.3 kb. Supplementary Figure S1. Yanagi, et al.
s1 A Pten flox/flox () SOPten flox/flox () flox allele 6. kb B Pten flox/flox () SOPten flox/flox () Pten Actin! allele 2.3 kb Supplementary Figure S1. Yanagi, et al. A B BrdU BrdU positive cells ( ) 3
Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at
Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at E10.5 were double-stained for TUNEL (red) and PECAM-1 (green).
Table S1. Primer sequences used for qrt-pcr. CACCATTGGCAATGAGCGGTTC AGGTCTTTGCGGATGTCCACGT ACTB AAGTCCATGTGCTGGCAGCACT ATCACCACTCCGAAGTCCGTCT LCOR
Table S1. Primer sequences used for qrt-pcr. ACTB LCOR KLF6 CTBP1 CDKN1A CDH1 ATF3 PLAU MMP9 TFPI2 CACCATTGGCAATGAGCGGTTC AGGTCTTTGCGGATGTCCACGT AAGTCCATGTGCTGGCAGCACT ATCACCACTCCGAAGTCCGTCT CGGCTGCAGGAAAGTTTACA
Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β
Supplementary Figures Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β and LPS. Non-parenchymal liver cells were isolated and treated with or without TGF-β
SUPPLEMENTARY INFORMATION. Supplementary Figures S1-S9. Supplementary Methods
SUPPLEMENTARY INFORMATION SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane Jian Huang 1,2#, Jie Yan 1,2#, Jian Zhang 3#, Shiguo Zhu 1, Yanli Wang
Supplementary Figures
Supplementary Figures Supplementary Figure 1. Confirmation of Dnmt1 conditional knockout out mice. a, Representative images of sorted stem (Lin - CD49f high CD24 + ), luminal (Lin - CD49f low CD24 + )
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3021 Supplementary figure 1 Characterisation of TIMPless fibroblasts. a) Relative gene expression of TIMPs1-4 by real time quantitative PCR (RT-qPCR) in WT or ΔTimp fibroblasts (mean ±
Supplementary Figure 1. SA-β-Gal positive senescent cells in various cancer tissues. Representative frozen sections of breast, thyroid, colon and
Supplementary Figure 1. SA-β-Gal positive senescent cells in various cancer tissues. Representative frozen sections of breast, thyroid, colon and stomach cancer were stained with SA-β-Gal and nuclear fast
Supplemental Table 1. Plasma NEFA and liver triglyceride levels in ap2-hif1ako and ap2-hif2ako mice under control and high fat diets.
Supplemental Table 1. Plasma NEFA and liver triglyceride levels in Hif1aKO and Hif2aKO mice under control and high fat diets. Hif1a (n=6) Hif1aK O (n=6) Hif2a Hif2aK O Hif1a (n=5) Hif1aKO (n=5) Hif2a Hif2aK
Supplementary Figure 1: STAT3 suppresses Kras-induced lung tumorigenesis
Supplementary Figure 1: STAT3 suppresses Kras-induced lung tumorigenesis (a) Immunohistochemical (IHC) analysis of tyrosine 705 phosphorylation status of STAT3 (P- STAT3) in tumors and stroma (all-time
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3311 A B TSC2 -/- MEFs C Rapa Hours WCL 0 6 12 24 36 pakt.s473 AKT ps6k S6K CM IGF-1 Recipient WCL - + - + - + pigf-1r IGF-1R pakt ps6 AKT D 1 st SILAC 2 nd SILAC E GAPDH FGF21 ALKPGVIQILGVK
TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer
Supplementary Information TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer Yabing Mu, Reshma Sundar, Noopur Thakur, Maria Ekman, Shyam Kumar Gudey, Mariya
Supplementary Information
Supplementary Information mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis Zhang et al. a b d e g h Rel. Luc. Act. Rel. mrna Rel. mrna
B16F1 B16F10 Supplemental Figure S1
B16F1 B16F1 Supplemental Figure S1 Representative microangiography images of B16F1 and B16F1 tumors grown in the cranial windows. FITC-dextran (2 million MW) was injected systemically to visualize blood
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic B cells from WT Nfat2 +/+, TCL1 Nfat2 +/+ and TCL1 Nfat2
Supplemental Information
Supplemental Information LKB1 inhibits lung cancer progression through lysyl oxidase and extracellular matrix remodeling Yijun Gao, Qian Xiao, HuiMin Ma, Li Li, Jun Liu, Yan Feng, Zhaoyuan Fang, Jing Wu,
condition. Left panel, the HCT-116 cells were lysed with RIPA buffer containing 0.1%
FIGURE LEGENDS Supplementary Fig 1 (A) sumoylation pattern detected under denaturing condition. Left panel, the HCT-116 cells were lysed with RIPA buffer containing 0.1% SDS in the presence and absence
Supplemental Figure S1. RANK expression on human lung cancer cells.
Supplemental Figure S1. RANK expression on human lung cancer cells. (A) Incidence and H-Scores of RANK expression determined from IHC in the indicated primary lung cancer subgroups. The overall expression
SUPPLEMENTARY FIGURE LEGENDS. atypical adenomatous hyperplasias (AAH); Grade II: adenomas; Grade III: adenocarcinomas;
SUPPLEMENTARY FIGURE LEGENDS Supplementary Figure S1: Tumor grades in Ras G12D ; p53 / lung tumors. Representative histology (H&E) of K-Ras G12D ; p53 / lung tumors 13 weeks after tumor initiation. Grade
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES 1 Supplementary Figure 1, Adult hippocampal QNPs and TAPs uniformly express REST a-b) Confocal images of adult hippocampal mouse sections showing GFAP (green), Sox2 (red), and REST
(a) Schematic diagram of the FS mutation of UVRAG in exon 8 containing the highly instable
Supplementary Figure 1. Frameshift (FS) mutation in UVRAG. (a) Schematic diagram of the FS mutation of UVRAG in exon 8 containing the highly instable A 10 DNA repeat, generating a premature stop codon
Supplementary Materials
Supplementary Materials Figure S1. MTT Cell viability assay. To measure the cytotoxic potential of the oxidative treatment, the MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide] assay
Supplementary Figure 1. Spatial distribution of LRP5 and β-catenin in intact cardiomyocytes. (a) and (b) Immunofluorescence staining of endogenous
Supplementary Figure 1. Spatial distribution of LRP5 and β-catenin in intact cardiomyocytes. (a) and (b) Immunofluorescence staining of endogenous LRP5 in intact adult mouse ventricular myocytes (AMVMs)
Supplementary Figure 1 Induction of cellular senescence and isolation of exosome. a to c, Pre-senescent primary normal human diploid fibroblasts
Supplementary Figure 1 Induction of cellular senescence and isolation of exosome. a to c, Pre-senescent primary normal human diploid fibroblasts (TIG-3 cells) were rendered senescent by either serial passage
Supplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1 Schematic depiction of the tandem Fc GDF15. Supplementary Figure 2 Supplementary Figure 2 Gfral mrna levels in the brains of both wild-type and knockout Gfral
Supplementary Information Titles Journal: Nature Medicine
Supplementary Information Titles Journal: Nature Medicine Article Title: Corresponding Author: Supplementary Item & Number Supplementary Fig.1 Fig.2 Fig.3 Fig.4 Fig.5 Fig.6 Fig.7 Fig.8 Fig.9 Fig. Fig.11
Supplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus
Supplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus changes in corresponding proteins between wild type and Gprc5a-/-
Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols
Supplementary Figure S1. Monolayer differentiation of mouse ESCs into telencephalic neural precursors. (a) Schematic representation of the protocols used to differentiate mouse ESCs. (b) Representative
Supplemental Data. TGF-β-mediated mir-181a expression promotes breast cancer metastasis by targeting Bim.
Supplemental Data TGF-β-mediated mir-181a expression promotes breast cancer metastasis by targeting Bim. Molly A. Taylor 1, Khalid Sossey-Alaoui 2, Cheryl L. Thompson 3, David Danielpour 4, and William
Cells and reagents. Synaptopodin knockdown (1) and dynamin knockdown (2)
Supplemental Methods Cells and reagents. Synaptopodin knockdown (1) and dynamin knockdown (2) podocytes were cultured as described previously. Staurosporine, angiotensin II and actinomycin D were all obtained
Tcf21 MCM ; R26 mtmg Sham GFP Col 1/3 TAC 8W TAC 2W. Postn MCM ; R26 mtmg Sham GFP Col 1/3 TAC 8W TAC 2W
A Tcf21 MCM ; R26 mtmg Sham GFP Col 1/3 Tcf21 MCM ; R26 mtmg TAC 2W Tcf21 MCM ; R26 mtmg TAC 8W B Postn MCM ; R26 mtmg Sham GFP Col 1/3 Postn MCM ; R26 mtmg TAC 2W Postn MCM ; R26 mtmg TAC 8W Supplementary
Nature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 EGFR inhibition activates signaling pathways (a-b) EGFR inhibition activates signaling pathways (a) U251EGFR cells were treated with erlotinib (1µM) for the indicated times followed
Supplementary Figure S1 Expression of mir-181b in EOC (A) Kaplan-Meier
Supplementary Figure S1 Expression of mir-181b in EOC (A) Kaplan-Meier curves for progression-free survival (PFS) and overall survival (OS) in a cohort of patients (N=52) with stage III primary ovarian
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2566 Figure S1 CDKL5 protein expression pattern and localization in mouse brain. (a) Multiple-tissue western blot from a postnatal day (P) 21 mouse probed with an antibody against CDKL5.
Supplemental Figure 1
Supplemental Figure 1 A S100A4: SFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDK Overlap: SF G DE KLM LD N D VDFQEY VFL I M N FF G PD S100A2: SFVGEKVDEEGLKKLMGSLDENSDQQVDFQEYAVFLALITVMCNDFFQGCPDR
Supplemental Table S1
Supplemental Table S. Tumorigenicity and metastatic potential of 44SQ cell subpopulations a Tumorigenicity b Average tumor volume (mm ) c Lung metastasis d CD high /4 8. 8/ CD low /4 6./ a Mice were injected
Supplementary Figure (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were
Supplementary Figure 1. Gd@C 82 (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were treated with PBS, Gd@C 82 (OH) 22, C 60 (OH) 22 or GdCl
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
Supplementary Figure 1. a. b. Relative cell viability. Nature Genetics: doi: /ng SCR shyap1-1 shyap
Supplementary Figure 1. a. b. p-value for depletion in vehicle (DMSO) 1e-05 1e-03 1e-01 1 0 1000 2000 3000 4000 5000 Genes log2 normalized shrna counts in T0 0 2 4 6 8 sh1 shluc 0 2 4 6 8 log2 normalized
Supplemental Figure 1. Intracranial transduction of a modified ptomo lentiviral vector in the mouse
Supplemental figure legends Supplemental Figure 1. Intracranial transduction of a modified ptomo lentiviral vector in the mouse hippocampus targets GFAP-positive but not NeuN-positive cells. (A) Stereotaxic
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure S1: Fibroblast-induced elongation of cancer cells requires direct contact with living fibroblasts. A. Representative images of HT29-GFP cultured in the presence
SUPPLEMENTARY DATA. Supplementary Table 1. Characteristics of Subjects.
Supplementary Table 1. Characteristics of Subjects. a includes one patient who had an aqueous sample taken from the same eye twice b includes one patients who had an aqueous sample taken from the same
Supplemental Data Macrophage Migration Inhibitory Factor MIF Interferes with the Rb-E2F Pathway
Supplemental Data Macrophage Migration Inhibitory Factor MIF Interferes with the Rb-E2F Pathway S1 Oleksi Petrenko and Ute M. Moll Figure S1. MIF-Deficient Cells Have Reduced Transforming Ability (A) Soft
Supplemental Information. Menin Deficiency Leads to Depressive-like. Behaviors in Mice by Modulating. Astrocyte-Mediated Neuroinflammation
Neuron, Volume 100 Supplemental Information Menin Deficiency Leads to Depressive-like Behaviors in Mice by Modulating Astrocyte-Mediated Neuroinflammation Lige Leng, Kai Zhuang, Zeyue Liu, Changquan Huang,
(A) SW480, DLD1, RKO and HCT116 cells were treated with DMSO or XAV939 (5 µm)
Supplementary Figure Legends Figure S1. Tankyrase inhibition suppresses cell proliferation in an axin/β-catenin independent manner. (A) SW480, DLD1, RKO and HCT116 cells were treated with DMSO or XAV939
ANGPTL2 increases bone metastasis of breast cancer cells through. Tetsuro Masuda, Motoyoshi Endo, Yutaka Yamamoto, Haruki Odagiri, Tsuyoshi
Masuda et al. Supplementary information for ANGPTL2 increases bone metastasis of breast cancer cells through enhancing CXCR4 signaling Tetsuro Masuda, Motoyoshi Endo, Yutaka Yamamoto, Haruki Odagiri, Tsuyoshi
Predictive PP1Ca binding region in BIG3 : 1,228 1,232aa (-KAVSF-) HEK293T cells *** *** *** KPL-3C cells - E E2 treatment time (h)
Relative expression ERE-luciferase activity activity (pmole/min) activity (pmole/min) activity (pmole/min) activity (pmole/min) MCF-7 KPL-3C ZR--1 BT-474 T47D HCC15 KPL-1 HBC4 activity (pmole/min) a d
Title: Smooth muscle cell-specific Tgfbr1 deficiency promotes aortic aneurysm formation by stimulating multiple signaling events
Title: Smooth muscle cell-specific Tgfbr1 deficiency promotes aortic aneurysm formation by stimulating multiple signaling events Pu Yang 1, 3, radley M. Schmit 1, Chunhua Fu 1, Kenneth DeSart 1, S. Paul
SUPPLEMENTARY INFORMATION
Figure S1 Treatment with both Sema6D and Plexin-A1 sirnas induces the phenotype essentially identical to that induced by treatment with Sema6D sirna alone or Plexin-A1 sirna alone. (a,b) The cardiac tube
Supplementary Fig. S1. Schematic diagram of minigenome segments.
open reading frame 1565 (segment 5) 47 (-) 3 5 (+) 76 101 125 149 173 197 221 246 287 open reading frame 890 (segment 8) 60 (-) 3 5 (+) 172 Supplementary Fig. S1. Schematic diagram of minigenome segments.
Supplementary Figures. T Cell Factor-1 initiates T helper 2 fate by inducing GATA-3 and repressing Interferon-γ
Supplementary Figures T Cell Factor-1 initiates T helper 2 fate by inducing GATA-3 and repressing Interferon-γ Qing Yu, Archna Sharma, Sun Young Oh, Hyung-Geun Moon, M. Zulfiquer Hossain, Theresa M. Salay,
Protection against doxorubicin-induced myocardial dysfunction in mice by cardiac-specific expression of carboxyl terminus of hsp70-interacting protein
Protection against doxorubicin-induced myocardial dysfunction in mice by cardiac-specific expression of carboxyl terminus of hsp70-interacting protein Lei Wang 1, Tian-Peng Zhang 1, Yuan Zhang 2, Hai-Lian
RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh-
1 a b Supplementary Figure 1. Effects of GSK3b knockdown on poly I:C-induced cytokine production. RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh- GSK3b) were stimulated
Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was
Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was painted on the shaved back skin of CBL/J and BALB/c mice for consecutive days. (a, b) Phenotypic presentation of mouse back skin
Supplemental Figure 1. (A) The localization of Cre DNA recombinase in the testis of Cyp19a1-Cre mice was detected by immunohistchemical analyses
Supplemental Figure 1. (A) The localization of Cre DNA recombinase in the testis of Cyp19a1-Cre mice was detected by immunohistchemical analyses using an anti-cre antibody; testes at 1 week (left panel),
Supplementary Figure 1: Tissue of Origin analysis on 152 cell lines. (a) Heatmap representation of the 30 Tissue scores for the 152 cell lines.
Supplementary Figure 1: Tissue of Origin analysis on 152 cell lines. (a) Heatmap representation of the 30 Tissue scores for the 152 cell lines. The scores summarize the global expression of the tissue
Figure S1: Effects on haptotaxis are independent of effects on cell velocity A)
Supplemental Figures Figure S1: Effects on haptotaxis are independent of effects on cell velocity A) Velocity of MV D7 fibroblasts expressing different GFP-tagged Ena/VASP family proteins in the haptotaxis
s u p p l e m e n ta ry i n f o r m at i o n
Figure S1 Characterization of tet-off inducible cell lines expressing GFPprogerin and GFP-wt lamin A. a, Western blot analysis of GFP-progerin- or GFP-wt lamin A- expressing cells before induction (0d)
BCL-W has a fundamental role in B cell survival and lymphomagenesis.
Thomas Jefferson University Jefferson Digital Commons Department of Cancer Biology Faculty Papers Department of Cancer Biology 2-1-2017 BCL-W has a fundamental role in B cell survival and lymphomagenesis.
Soft Agar Assay. For each cell pool, 100,000 cells were resuspended in 0.35% (w/v)
SUPPLEMENTARY MATERIAL AND METHODS Soft Agar Assay. For each cell pool, 100,000 cells were resuspended in 0.35% (w/v) top agar (LONZA, SeaKem LE Agarose cat.5004) and plated onto 0.5% (w/v) basal agar.
Supplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
SUPPLEMENTAL EXPERIMENTAL PROCEDURES
SUPPLEMENTAL EXPERIMENTAL PROCEDURES Crystal violet assay Cells were seeded in 24-well plates and cultured in media supplemented with % FBS for 7 days. Media were then removed, plates were briefly washed
stability and tumor suppression
Supplementary information The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression Daniel Schramek 1, Athanassios Kotsinas 2, Arabella Meixner 1, Teiji Wada 1, Ulrich Elling
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Tables. File Name: Peer Review File Description:
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Tables File Name: Peer Review File Description: Primer Name Sequence (5'-3') AT ( C) RT-PCR USP21 F 5'-TTCCCATGGCTCCTTCCACATGAT-3'
Supplementary Materials for
www.sciencesignaling.org/cgi/content/full/8/364/ra18/dc1 Supplementary Materials for The tyrosine phosphatase (Pez) inhibits metastasis by altering protein trafficking Leila Belle, Naveid Ali, Ana Lonic,
Supplementary Figure 1:
Supplementary Figure 1: (A) Whole aortic cross-sections stained with Hematoxylin and Eosin (H&E), 7 days after porcine-pancreatic-elastase (PPE)-induced AAA compared to untreated, healthy control aortas
Supplemental Tables and Figures. The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate,
Supplemental Tables and Figures The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate, tendon-specific protective mechanism against heterotopic ossification Timothy Mead et al Supplemental
Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and
Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and exclude YAP from the nucleus. (a) Schematic diagram of an E10.5 mouse embryo. (b,c) Sections at B and C in (a)
Supplemental Information
Supplemental Information Tobacco-specific Carcinogen Induces DNA Methyltransferases 1 Accumulation through AKT/GSK3β/βTrCP/hnRNP-U in Mice and Lung Cancer patients Ruo-Kai Lin, 1 Yi-Shuan Hsieh, 2 Pinpin
Supplementary Table 1. The primers used for quantitative RT-PCR. Gene name Forward (5 > 3 ) Reverse (5 > 3 )
770 771 Supplementary Table 1. The primers used for quantitative RT-PCR. Gene name Forward (5 > 3 ) Reverse (5 > 3 ) Human CXCL1 GCGCCCAAACCGAAGTCATA ATGGGGGATGCAGGATTGAG PF4 CCCCACTGCCCAACTGATAG TTCTTGTACAGCGGGGCTTG
Lentiviral Delivery of Combinatorial mirna Expression Constructs Provides Efficient Target Gene Repression.
Supplementary Figure 1 Lentiviral Delivery of Combinatorial mirna Expression Constructs Provides Efficient Target Gene Repression. a, Design for lentiviral combinatorial mirna expression and sensor constructs.