FT-IR Analysis of column extract (F4) of B.zeylanica. FT-IR Analysis of column extract (F5) of B.zeylanica

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1 Fig. 39 FT-IR Analysis of column extract (F4) of B.zeylanica Fig. 40 FT-IR Analysis of column extract (F5) of B.zeylanica

2 Fig. 41 FT-IR Analysis of column extract (F1) of P.persica Fig. 42 FT-IR Analysis of column extract (F2) of P.persica

3 Fig. 43 FT-IR Analysis of column extract (F3) of P.persica Fig. 44 FT-IR Analysis of column extract (F5) of P.persica

4 Fig. 45 FT-IR Analysis of column extract (F1) of C.virgineus Fig. 46 FT-IR Analysis of column extract (F2) of C.virgineus

5 Fig HNMR Spectrum of column extract (F4) of B.zeylanica Expanded

6 Fig HNMR Spectrum of column extract (F5) of B.zeylanica Expanded

7 Fig HNMR Spectrum of column extract (F1) of P.persica Expanded

8 Fig HNMR Spectrum of column extract (F2) of P.persica Expanded

9 Fig HNMR Spectrum of column extract (F3) of P.persica Expanded

10 Fig HNMR Spectrum of column extract (F5) of P.persica Expanded

11 Fig HNMR Spectrum of column extract (F1) of C.virgineus Expanded

12 Fig HNMR Spectrum of column extract (F2) of C.virgineus Expanded

13 Fig. 55 Chromatogram of column extract (F4) of B.zeylanica by GC-MS Fig. 55 a Chromatogram Compounds in F4 fraction of B.zeylanica á-d-mannofuranoside, farnesyl- HO C 21 H 36 O HO O O OH OH (mainlib) á-d-mannofuranoside, farnesyl- Fig. 55 b 2-Piperidnone C 5 H 9 NO

14 Fig. 55 c 9-Octadecenamide, (z)- C18H35NO Fig. 55 d 1,2-Benzenedicarboxylic acid, diisooctyl ester C 24H 38O 4 Fig. 56 Chromatogram of column extract (F5) of B.zeylanica by GC-MS

15 Fig. 56 a Chromatogram Compounds in F5 fraction of B.zeylanica (mainlib) 2,2-Dimethyl-6-methylene-1-[3,5-dihydroxy-1-pentenyl]cyclohexan-1-perhydrol Fig. 56 b 2,2-Dimethyl-6-methylene-1-[3,5-dihydroxy-1- pentenyl]cyclohexan-1-perhydrol 160 O OH 203 OH OH 221 C 14 H 24 O 4 Diethyl Phthalate C 12 H 14 O 4 Fig. 56 c trans-à-bergamotene C15H (mainlib) trans-à-bergamotene

16 Fig. 57 Chromatogram of column extract (F1) of P.persica by GC-MS Chromatogram Compounds in F1 fraction of P.persica Fig. 57 a 7,8 Epoxylanostan-11-ol, 3-acetoxy C32H54O4 Fig. 57 b Ethanethiol, 2-phenoxy- C8H10OS

17 Fig. 58 Chromatogram of column extract (F2) of P.persica by GC-MS Fig. 58 a Chromatogram Compounds in F2 fraction of P.persica 9,12-Octadecadienoyl chloride, (Z,Z)- C18H31ClO Chromatogram of column extract (F3) of P.persica by GC-MS Fig. 59

18 Fig. 59 a Chromatogram Compounds in F3 fraction of P.persica Eugenol C10H12O2 Fig. 59 b 1,2-Benzenedicarboxylic acid, diisooctyl ester C24H38O4 Fig. 59 c Phthalic acid, bis (7-methyloctyl) ester C26H42O4

19 Chromatogram of column extract (F5) of P.persica by GC-MS Fig. 60 Chromatogram Fig. 60 a Compounds in F5 fraction of P.persica Azulene C10H8 Fig. 60 b 1-Octanol, 2,7-dimethyl- C10H22O

20 Fig. 60 c 2-Piperidinone C5H9NO Fig. 60 d Cholest-5-ene, 3-bromo-, (3.beta.)- C27H45Br Chromatogram of column extract (F1) of C.virgineus by GC-MS Fig. 61

21 Fig. 61 a Chromatogram Compounds in F1 fraction of C.virgineus Diethyl Phthalate C12H14O4 Fig. 61 b Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro- C7H10N2O2 Fig. 61 c 9-Dodecenoic acid, methyl ester, (E)- C13H24O2

22 Chromatogram of column extract (F2) of C.virgineus by GC-MS Fig. 62 Fig. 62 a Chromatogram Compounds in F2 fraction of C.virgineus Perfluoro-n-pentanoic acid C5HF9O2 Fig. 62 b Perfluorotributylamine C12F27N

23 Fig. 62 c Ethanol, 2-(hexadecyloxy)- C18H38O2

24 Fig. 63 HPLC chromatogram of isolated compound of B.zeylanica compared to the standard diethyl phthalate

25 Fig. 64 HPLC chromatogram of isolated compound of B.zeylanica compared to the standard oleic acid

26 Fig. 65 HPLC chromatogram of isolated compound of P.persica compared to the standard azulene

27 Fig. 66 HPLC chromatogram of isolated compound of P.persica compared to the standard eugenol

28 Fig. 67 HPLC chromatogram of isolated compound of P.persica compared to the standard oleic acid

29 Fig. 68 HPLC chromatogram of isolated compound of P.persica compared to the standard diethyl phthalate

30 Fig. 69 HPLC chromatogram of isolated compound of C.virgineus compared to the standard oleic acid

31 Fig. 70 HPLC chromatogram of isolated compound of C.virgineus compared to the standard diethyl phthalate

32 Table 1 Showing various activities of identified compounds by GC-MS in experimental animals B.zeylanica, P.persica and C.virgineus B.zeylanica (F 4 ) Benzene : Methanol S.No RT Name of the compound Peak Area % Glycerin Alcohol Piperidinone 6.55 Alkaloid Diethyl Phthalate Hexadecenoic acid, methyl ester, (Z)- Hexadecanoic acid, methyl ester Compound Nature Plasticizer compound 1.83 Oleic acid ester 4.66 Palmitic acid ester * Activity Octadecenamide, (Z) Amide compound Undecanal, 2-methyl Aldehyde compound Oleic Acid 0.73 Oleic acid ,2-Benzenedicarboxylic acid, diisooctyl ester 3-Hexadecyloxycarbonyl- 5-(2-hydroxyethyl)-4- methylimidazolium ion á-d-mannofuranoside, farnesyl Plastici8zer compound 3.88 Nitrogen compound Terpene compound Cholesterol Steroid Preservative Anti-inflammatory Anti fouling Anti-inflammatory, Antiandrogenic, Cancer preventive, Dermatitigenic, Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic, Insectifuge, Flavor Antioxidant, Hypocholesterolemic Nematicide, Pesticide, Lubricant, Antiandrogenic, Flavor, Hemolytic 5-Alpha reductase inhibitor Anti-inflammatory, Antiandrogenic, Cancer preventive, Dermatitigenic, Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic, Insectifuge, Flavor Anti fouling Anti-inflammatory Anticancer Antiasthma Anti-inflammatory

33 Table 2 B.zeylanica (F 5 ) Methanal RT Name of the compound Peak Area % Compound Nature trans-à-bergamotene 2.35 Sesquiterpene Diethyl Phthalate 4.70 Plasticizer compound Phenol, 2-methyl-5- (1,2,2- trimethylcyclopentyl)-, (S)- 1,2- Benzenedicarboxylic acid, diisooctyl ester 2,2-Dimethyl-6- methylene-1-[3,5- dihydroxy-1- pentenyl]cyclohexan- 1-perhydrol 2.98 Phenolic compound Plasticizer compound *Activity Anti-inflammatory Antifouling Anti-inflammatory Antioxidant Antifouling 5.78 Alcoholic compound Table 3 P.persica (F 1 ) Hexane : Chloroform S.No RT Name of the compound Peak Area % ,8-Epoxylanostan-11-ol, 3-acetoxy Nonadecanone 2,4-dinitrophenylhydrazine 8.79 Compound Nature Alcoholic compound Nitrogen compound *Activity Ethanethiol, 2-phenoxy Sulfur compound

34 Table 4 P.persica (F 2 ) Chloroform S.No RT Name of the compound Peak Area % Nature of compound *Activity ,12-Octadecadienoyl chloride, (Z,Z) Chloride compound ,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)- Cholest-5-en-3-ol (3á)-, carbonochloridate 1.52 Linoleic acid ester Steroid Anti-inflammatory, Hypocholesterolemic, Cancer preventive Hepatoprotective Nematicide, Insectifuge Antihistaminic, Antieczemic Antiacne, 5-Alpha reductase inhibitor Antiandrogenic, Antiarthritic, Anticoronary, Insectifuge Antiasthma Anti-inflammatory Table 5 P.persica No RT Name of the compound Peak Area % Eugenol Dibutyl phthalate ,2- Benzenedicarboxylic acid, diisooctyl ester Phthalic acid, bis(7- methyloctyl) ester Compound Nature Phenolic compound Plasticizer compound Plasticizer compound Plasticizer compound (F 3 ) Benzene *Activity Analgesic, Anesthetic, Allergenic, Antibacterial, Anticonvulsant, Anti-inflammatory, Antioxidant, Antipyretic, Antisalmonella, Antistaphylococc, Antiseptic Antifouling Antifouling Antifouling

35 Table 6 P.persica (F 5 ) Methanol S.No RT Name of the compound Peak Area % Nature of compound Glycerin Alcoholic compound Piperidinone Alkaloid compound Acetic acid, trifluoro-, tetradecyl ester *Activity Preservative Anti-inflammatory 4.88 Fluro compound Octanol, 2,7-dimethyl Alcoholic compound Azulene 0.55 Monoterpene Cholest-5-ene, 3- bromo-, (3á) Steroid Anti-tumor, Analgesic, Antibacterial Antiinflammatory, Sedative, Fungicide, Hypocholesterolemic, Insecticide, Insectifuge Chemopreventive, Pesticide, Antiacne, Nematicide Anti-inflammatory Anticancer Diuretic Antiasthma

36 Table 7 C.virgineus (F 1 ) Hexane: Chloroform No RT Name of the compound Dimethylaminomethylisopropyl-sulfide Peak Area % 4.75 Compound Nature Amino compound with sulfur Piperidinone 0.39 Alkaloid Diethyl Phthalate Pyrrolo[1,2-a]pyrazine-1,4- dione, hexahydro Hexadecanoic acid, methyl ester Dodecenoic acid, methyl ester, (E) ,8,11,14- Eicosatetraenoic acid, ethyl ester, (all-z) ,11,14-Eicosatrienoic acid, (Z,Z,Z) ,10-Secochola- 5,7,10(19)-trien-24-al, 3- hydroxy-, (3á,5Z,7E)- Plasticizer compound 0.59 Alkaloid 2.58 Palmitic acid ester 0.88 Oleic acid ester Unsaturated fatty acid ester Unsaturated fatty acid ester 7.24 Steroid * Activity Anti-inflammatory Anti fouling Anti-inflammatory Antioxidant, Pesticide, Hypocholesterolemic, Nematicide, Lubricant, Antiandrogenic, Flavor, Hemolytic. Anti-inflammatory, Antiandrogenic Cancer preventive, Dermatitigenic Hypocholesterolemic, 5-Alpha reductase inhibitor, Anemiagenic Insectifuge, Flavor Anticholesterol Anticholesterol Anti-inflammatory Antiasthma Diuretic

37 Table 8 C.virgineus (F 2 ) Chloroform No RT Name of the compound Peak Area % Compound Nature * Activity Diethyl Phthalate Plasticizer compound Antifouling Perfluorotributylamine 4.50 Amino compound Perfluoro-n-pentanoic acid 7.19 Fluro compound Ethanol, 2- (hexadecyloxy) Alcoholic compound * Source: Dr.Duke's Phytochemical and Ethnobotanical Databases

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