The University of ~ukurova, Art & Science Faculty, Department of Chemistry, BaIcali, Adana-TURKEY

Size: px
Start display at page:

Download "The University of ~ukurova, Art & Science Faculty, Department of Chemistry, BaIcali, Adana-TURKEY"

Transcription

1 BIOCHEMISTRY andmolecular BIOLOGY INTERNATIONAL pages EFFECTS OF SULFHYDRYL COMPOUNDS ON THE INHIBITION OF ERYTHROCYTE MEMBRANE Na+-K + ATPase BY OZONE Rmnazan Bilgin, Sermin Gill, S. Seyhan Ttikel The University of ~ukurova, Art & Science Faculty, Department of Chemistry, BaIcali, Adana-TURKEY Received June 22, 1998 Accepted September 29, 1998 SUMMARY Exposure of human erythrocyte membranes to ozone (5 gmol/10 min) resulted in the inhibition of erythrocyte membrane Na+-K + ATPase (EC ). It was determined that, the degree of enzyme inhibition in the directly ozone exposed membranes was greater than that of membranes obtained from ozone exposed intact erythrocytes. In the presence of varying concentrations (0-1.0 ram) of dithiotrethiol or mercaptoethanol Na+-K + ATPase activities of both types of ozone exposed membranes were increased almost proportionally with the concentration of dithiotrethiol or mercaptoethanol however, the activities were still lower than the normal Na+-K + ATPase value. The results indicate that, dithiotrethiol or mercaptoethanol prevent the enzyme inhibition by ozone in vitro. This suggests that the membrane thiol groups are primary targets for ozone and thereby preventing the oxidation of essential functional groups of enzyme,protein. Key words: Ozone, erythrocyte, sulfhydryl compounds, Na+-K + ATPase INTRODUCTION Because ozone is a very reactive agent, every biomolecule may be a potential target for reaction with ozone. There are many in vitro and in vivo studies on the oxidative damage of ozone to biological systems (1, 2, 3, 4). In studies, in vitro erythrocytes have been used as a well-characterized model cell. It has been shown that the membrane constituents, lipids, enzymes and other essential proteins were susceptible to oxidation by ozone. The relationship between polyunsaturated fatty acid peroxidation and protein oxidation is further complicated by the fact that the maintenence of the tertiary structures of cellular membrane proteins is dependent on their associated lipids. Alterations in lipids surrounding the embedded proteins may result in structural alterations and changes in membrane function (5). Earlier reports (6,7) showed that exposure of erythrocytes or their membranes to low lewels of ozone 227 Copyright UBMB /99 $

2 BIOCHEMISTRY andmolecular BIOLOGY INTERNATIONAL lead to a loss of the Na+-K + ATPase activity as a result of a) destruction of phospholipids, b) cross linking of the membrane proteins by lipid peroxidation products, c) oxidation of essential sulphydryl groups and d) destruction of other essential components of enzyme complex. Several plasma ezymes such as lactate dehydrogenase, alkaline phosphatase, gamma glutamyl transferase and aspartate transaminase were studied in ozone exposed blood samples. The results suggested that exposure to ozone may cause oxidative damage to proteins in human body fluids (8). This study was aimed at determining the effects of dithiothreitol and mercaptoethanol on the inhibition of erythrocyte membrane Na+-K + ATPase by ozone. MATERIALS AND METHODS Materials: All reagents were of highest purity available and were obtained from BDH (England), Merck (Germany) or Sigma Chemical Company (U.S.A.). Blood samples were obtained from the BloOd Bank of ~ukurova University Medical Faculty. Preparation of ozone exposed erythrocytes and preparation of membranes from ozone exposed erythrocytes: Erythrocytes were washed four times with 10 mm potassium phosphate buffer, (ph 7.4) containing 135 mm NaC1. The cells were suspended to 10% by volume in the same buffer solution. A series of 50 ml cell suspension having the final dithiothreitol or mercaptoethanol concentrations of 0.0, 0.1, 0.3, 0.5, 0.7, 1.0 mm were prepared. Each of the samples were then immediately exposed to a flow of 5 gmolo3/min. The ozone exposed samples were centrifuged at 5000 g for 5 rain to sediment the elythrocytes which were rewashed four times with 0.9 % NaC1 solution. Then, membranes of these ozone exposed eltthrocytes were prepared according to the procedure described by (9) with some modifications. Briefly, ozone exposed cells were hemolyzed in an ice bath by adding them into 5 volumes of 10 mm Tris buffer (ph 7.4) containing 1 mm EDTA and centrifuged at g for 10 min. The membranes were washed with the same buffer until the supernatant fluid was colorless. The membrane fragments were finally suspended in the same buffer at a concentration of 3.0 mg of protein/ml in a series of tubes and were stored at -20~ until used. Preparation of membranes from intact erythrocytes and exposure of these membranes to ozone: Erythrocytes were washed four times with 10 mm potassium phosphate buffer, (ph 7.4) containing 135 mm NaCt. The cells were suspended to 10% by volume in the same buffer solution. Membranes of washed intact erythrocytes were prepared according to the procedure described above. An aliquot of this membrane suspension was used to detmrnine the Na+-K ATPase activity which was designated the control value ( gmol Pi/mg prot./h). 10 ml portions of this membrane suspension were immediately exposed to ozone again in the presence of (0.0, 0.1, 0.3, 0.5, 0.7, 1.0) mm dithiothreitol or the same concentration range of mercaptoethanol. Ozone generation: Ozone was generated by a Fisher-Ozone Generator Model 50I at an ozone flow rate 5 gmol O3/min. Ozone concentration was measured by titration with KI- Na2S203 - starch in 5 mm sodium phosphate (ph 7.0). 228

3 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Measurement of Na + ATPase activity: Na+-K + ATPase was assayed as the release of inorganic phosphate (Pi) from ATP according to the method described by (10). Assays were carried out in a final volume of 2.5 ml containing 0.3 mg membrane protein as the enzyme sotu-ce, 120 mm NaC1, 10 mm KCI, 35 mm Tris HC1 buffer (ph 7.4), 3 mm NazATP and the presence or absence of 0.2 mm ouabain. Membranes were preincubated in the mixture for 10 min at 37~ before starting the reaction by adding the substrate, Na2ATP. After 30 rain incubation with substrate reaction was stopped by adding 0.5 ml of 30 % trichloroacetic acid. The released Pi was assayed by the method of (11) and protein was measured by the method of (12). Na+-K + ATPase activity was calculated by subtracting the activity in the presence of ouabain from the activity in its absence. Enzyme activity is expressed as gmol Pi/mg prot./h. RESULTS AND DISCUSSION The Na+-K + ATPase activity of membranes prepared from normal erythrocytes was gmol Pi/mg prot./h(n:10). This value was considered as the control value. The Na+-K + ATPase activities of the membranes prepared from the erythrocytes exposed to ozone in the presence of varying concentrations (0-1.0 mm) of dithiothretiol or mercaptoethanol and the membranes prepared from the erythrocytes and then exposed to ozone in the presence of varying concentrations (0-1.0 ram) of dithiothretiol or mercaptoethanol are presented in Figures 1, 2. ~" 0.30 e~ " ~ 0.20 "7..< [,,,,, < o i i i i Concentration (ram) 1.2 Fig 1. Na+-K + ATPase activities of membranes prepared from erythrocytes, which had prior exposure to ozone for 10 rain, in the presence of varying concentrations of mercaptoethanol and dithiothreitol mercaptoethanol --A-- dithiothreitol 229

4 BIOCHEMISTRY andmolecular BIOLOGY INTERNATIONAL Ozone exposure time was chosen as 10 min, because a previous study (13) showed that the Na+-K + ATPase activity was inhibited by approximately 70 % in membrane preparation from ozone exposed erythrocytes or membranes exposed to ozone for 10 rain. Figures 1, 2 Show that the presence of dithiothretiol or mercaptoethanol prevents the ozone inhibition of Na+-K + ATPase almost in the same range. The Na+-K + ATPase activities of the membranes prepared from the erythrocytes exposed to ozone in the presence of 1.0 mm dithiothreitol ( gmol Pi/mg prot./h ) was found to be 2.25 times greater than that of the Na+-K + ATPase activity of the membranes prepared from the erythrocytes exposed to ozone in the absence of dithiothreitol (0.120 _ lamol Pi/mg prot./h ). Similar results were obtained in the presence of mercaptoethanol. Na+-K + ATPase activity of the membranes prepared from the erythrocytes exposed to ozone in the presence of 1.0 mm mercaptoethanol ( gmol Pi/mg prot./h) was 2.1 times greater than that of ( gmol Pi/mg prot./h) the corresponding membrane preparation in the absence of mercaptoethanol. '~ ;;, '~. 'i 0.15 & 0.10 I., 9 ; 9 ~ 9,, ; Z Concentration (mm) 1.2 Fig 2, Na+-K + ATPase activities of isolated erythrocyte membranes which were then exposed to ozone for t0 rain in the presence of varying concentrations of mercaptoethanol and dithioth~eitol. --D-- --A-- mercaptoethanol dithiothreitol 230

5 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL The Na+-K + ATPase activities of the membranes prepared from the intact erythrocytes and then exposed to ozone in the presence of 1.0 mm dithiothreitol (0.27 _ lamol Pi/mg prot./h) or 1 mm mercaptoethanol ( gmol Pi/mg prot./h) were 2.1 or 2.0 times greater than that of the Na+-K + ATPase activity of the membranes treated with ozone in the absence of these reducing agents. In earlier studies, it was reported that exposure of erythrocytes in vitro, or their membranes to ozone lead to a loss of the Na+-K + ATPase activity of the erythrocyte membranes (3, 13) and it is possible that ozone oxidized plazma proteins directly by attacking histidine and tyrosine residues in proteins (14, 15). Oxidative damage to proteins as well as to lipids should be a consideration in attempting to understand the mechanism of ozone toxicity and in the design of suitable protective agents. The results suggest that, in vitro, dithiothreitol and mercaptoethanol prevent the inhibition of Na+-K + ATPase by ozone. This may be due to the fact that their thiol groups are preferentially oxidized by ozone thereby limiting the oxidation of some essential functional groups of the enzyme protein. The actions of dithiothreitol, and mercaptoethanol were similar in their opposition to the inhibitory effects of ozone, although the protective effect of mercaptoethanol was marginally greater than that of dithiothreitol in all cases. Acknowledgement This work supported by Research Grant (DTP-TBAG/49) fi'om Turkish Reseaxch and Scientific Council. REFERENCES 1. Moore, R. B., Brummitt, L. M. and Mankad, V. N. (1984) Arch. Biochem. Biophys. 273 (2), Van, D. Z., Christanse, J. T., Dubbelman, K. and Steveninok, V. J. (t987) Biochim. Biophys. Acta. 924, Kesner, L., Kindya, R. J. and Chan, P. C. (1979) J. Biol. Chem. 254 (9), Goldstein, B. D. (1979) Oxygen Free Radicals Tissue Damage, pp , Elsevier, NewYork. 5. Borek, C. and Mehlman, M. A. (1983) In The Biomedical Effects of Ozone and Related Photochemical Oxidants (Lee S. D., Mustafa, M. G. and Mehlman. M. A., Eds.), pp , Princeton Scientific Publishers, Princeton. N. J. 6. Kindya, R. J and Chan, P. C (1976) Biochim. Biophys. Acta. 429, Cross, E. C., Reznick, A. Z., Packer, L., Davis, A. P., Suziki, J. Y. and Halliwell, B. (1992) Free Radical Res. Com. 15, Hanahan, U. J. and Eckholm, J. E. (1974) Method Enzymol. 31, Luly, P., Baldini, P., Incerpi, S. and Tria, E. (1981) Experienta 37, Atkinson, A., Gatenby, A. D. and Lowe, A. G. (1973) Biocbem. Biophys. Acta. 320,

6 BIOCHEMISTRYandMOLECULAR BIOLOGY INTERNATIONAL 12. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randal, R. J. (1951) J. Biol. Chem. 193, Ttikel, S. S., Bilgin, R., Gtil, ~. (1994) Biochem. Mol. Biol. Int. 33(6), t Berlett, B. A, Omar, O. H. M., Sahakian, J. A., Levine, R. L. and Stadtman, E.R. (1991) FASEB J. 5, A Verweij, H., Christianse, K. and Van Steveninck, J. V. (1982) Biochim. Biophys. Acta. 701,

B. 15 mm Ouabain Solution (Ouabain) (Prepare 10 ml in Reagent A using Ouabain Octahydrate, Sigma Prod. No. O3125.)

B. 15 mm Ouabain Solution (Ouabain) (Prepare 10 ml in Reagent A using Ouabain Octahydrate, Sigma Prod. No. O3125.) SIGMA QUALITY CONTROL TEST PROCEDURE Sigma Prod. No. A7510 PRINCIPLE: ATP + H 2 O ATPase > ADP + P i Abbreviations used: ATPase = Adenosine 5'-Triphosphatase ATP = Adenosine 5'-Triphosphate ADP = Adenosine

More information

Activation of Mitochondrial Glycerol 3-Phosphate Dehydrogenase by Cadmium Ions

Activation of Mitochondrial Glycerol 3-Phosphate Dehydrogenase by Cadmium Ions Gen. Physiol. Biophys. (1985), 4, 29 34 29 Activation of Mitochondrial Glycerol 3-Phosphate Dehydrogenase by Cadmium Ions H. RAUCHOVÁ, P. P. KAUL* and Z. DRAHOTA Institute of Physiology, Czechoslovak Academy

More information

1G. Celed6n*, ~V. Lips, 1C. Alvarado, 1M. Cort6s, 2E. A. Lissi and 3G. Gonzfilez

1G. Celed6n*, ~V. Lips, 1C. Alvarado, 1M. Cort6s, 2E. A. Lissi and 3G. Gonzfilez Vol. 43, No. 5, December 1997 Pages 1121-1 ] 27 PROTEIN DEGRADATION IN RED CELLS EXPOSED TO 2,2'-AZO-BIS(2-AMIDINOPROPANE) DERIVED RADICALS 1G. Celed6n*, ~V. Lips, 1C. Alvarado, 1M. Cort6s, 2E. A. Lissi

More information

Interaction of lanthanum chloride with human erythrocyte membrane in relation to acetylcholinesterase activity

Interaction of lanthanum chloride with human erythrocyte membrane in relation to acetylcholinesterase activity J. Biosci., Vol. 13, Number 2, June 1988, pp. 123 128. Printed in India. Interaction of lanthanum chloride with human erythrocyte membrane in relation to acetylcholinesterase activity SUNIL MUKHOPADHYAY,

More information

Phospholipid Assay Kit

Phospholipid Assay Kit Phospholipid Assay Kit Catalog Number KA1635 100 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Proteases in germinating finger millet (Eleusine coracana) seeds

Proteases in germinating finger millet (Eleusine coracana) seeds Biosci., Vol. 5, Number 3, September 1983, pp. 219 224. Printed in India. Proteases in germinating finger millet (Eleusine coracana) seeds Introduction U. VIDYAVATHI, B. SHIVARAJ and T. N. PATTABIRAMAN

More information

Effect of a Selenium Analogue of [L Title Transport of Candida pelliculosa (C Dedicated to Professor Masaya Okano Retirement) Author(s) Shimizu, Eiichi; Yamana, Ryutaro; T Kenji Citation Bulletin of the

More information

Stimulation of Active K + Transport by Anti-L Antibodies in Trypsin-Treated Low Potassium Sheep Erythrocytes

Stimulation of Active K + Transport by Anti-L Antibodies in Trypsin-Treated Low Potassium Sheep Erythrocytes LETTER TO THE EDITOR Stimulation of Active K + Transport by Anti-L Antibodies in Trypsin-Treated Low Potassium Sheep Erythrocytes Dear Sir: In this letter we attempt to resolve a discrepancy on the effect

More information

Iron Chelates and Unwanted Biological Oxidations

Iron Chelates and Unwanted Biological Oxidations The Virtual Free Radical School Iron Chelates and Unwanted Biological Oxidations Kevin D. Welch and Steven D. Aust Department of Chemistry and Biochemistry Biotechnology Center Utah State University Logan,

More information

Naoki YAMANAKA, Toshio IMANARI,* Zenzo TAMURA,*

Naoki YAMANAKA, Toshio IMANARI,* Zenzo TAMURA,* J. Biochem., 73, 993-998 (1973) Uncoupling of Oxidative Phosphorylation of Rat Liver Mitochondria by Chinoform Naoki YAMANAKA, Toshio IMANARI,* Zenzo TAMURA,* and Kunio YAGI Institute of Biochemistry,

More information

Synopsis. Received March 2, adrenaline. Mosinger and Kujalova (1964) reported that adrenaline-induced lipolysis

Synopsis. Received March 2, adrenaline. Mosinger and Kujalova (1964) reported that adrenaline-induced lipolysis Studies on Reduction of Lipolysis in Adipose Tissue on Freezing and Thawing YASUSHI SAITO1, NoBUO MATSUOKA1, AKIRA KUMAGAI1, HIROMICHI OKUDA2, AND SETSURO FUJII3 Chiba University, Chiba 280, Japan, 2Department

More information

erythrocyte membranes (transport/inhibition/isozyme)

erythrocyte membranes (transport/inhibition/isozyme) Proc. Nad. Acad. Sci. USA Vol. 84, pp. 7373-7377, November 1987 Biochemistry Glutathione disulfide-stimulated Mg2+-ATPase of human erythrocyte membranes (transport/inhibition/isozyme) TAKAHITO KONDO*,

More information

RAT LIVER MICROSOMES can be shown to carry out. lipid synthesis on added protein. Dependence of microsomal

RAT LIVER MICROSOMES can be shown to carry out. lipid synthesis on added protein. Dependence of microsomal Dependence of microsomal lipid synthesis on added protein RUTH TZUR and B. SHAPIRO Department of Biochemistry, The Hebrew University-Hadassah Medical School, Jerusalem, Israel SUMMARY Lipid synthesis by

More information

Oxidation of Escherichia coli Sulfhydryl Components by the Peroxidase-Hydrogen Peroxide-Iodide Antimicrobial System

Oxidation of Escherichia coli Sulfhydryl Components by the Peroxidase-Hydrogen Peroxide-Iodide Antimicrobial System ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, June 1978, p. 06-0066-4804/78/0013-06$02.00/0 Copyright 1978 American Society for Microbiology Vol. 13, No. 6 Printed in U.S.A. Oxidation of Escherichia coli Sulfhydryl

More information

BIL 256 Cell and Molecular Biology Lab Spring, Tissue-Specific Isoenzymes

BIL 256 Cell and Molecular Biology Lab Spring, Tissue-Specific Isoenzymes BIL 256 Cell and Molecular Biology Lab Spring, 2007 Background Information Tissue-Specific Isoenzymes A. BIOCHEMISTRY The basic pattern of glucose oxidation is outlined in Figure 3-1. Glucose is split

More information

ACTIVATION OF THE RABBIT POLYMORPHONUCLEAR

ACTIVATION OF THE RABBIT POLYMORPHONUCLEAR Published Online: 1 May, 1978 Supp Info: http://doi.org/10.1083/jcb.77.2.329 Downloaded from jcb.rupress.org on June 29, 2018 ACTIVATION OF THE RABBIT POLYMORPHONUCLEAR LEUKOCYTE MEMBRANE "Na+,K +''- ATPase

More information

Supplementary Files S1 Isolation of Monocytes S2 Haemolysis study Reagents Procedure S3 Cytotoxicity studies Trypan blue dye exclusion method

Supplementary Files S1 Isolation of Monocytes S2 Haemolysis study Reagents Procedure S3 Cytotoxicity studies Trypan blue dye exclusion method Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supplementary Files S1 Isolation of Monocytes A 3 ml volume of Histopaque 1083 solution was

More information

TRANSPORT OF AMINO ACIDS IN INTACT 3T3 AND SV3T3 CELLS. Binding Activity for Leucine in Membrane Preparations of Ehrlich Ascites Tumor Cells

TRANSPORT OF AMINO ACIDS IN INTACT 3T3 AND SV3T3 CELLS. Binding Activity for Leucine in Membrane Preparations of Ehrlich Ascites Tumor Cells Journal of Supramolecular Structure 4:441 (401)-447 (407) (1976) TRANSPORT OF AMINO ACIDS IN INTACT 3T3 AND SV3T3 CELLS. Binding Activity for Leucine in Membrane Preparations of Ehrlich Ascites Tumor Cells

More information

BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 48]-486

BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 48]-486 Vol. 41, No. 3, March 1997 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 48]-486 INACTIVATION OF ACONITASE IN YEAST EXPOSED TO OXIDATIVE STRESS Keiko Murakami and Masataka Yoshino* Department

More information

Gen. Physiol. Biophys. (1987). 6,

Gen. Physiol. Biophys. (1987). 6, Gen. Physiol. Biophys. (1987). 6, 103 108 103 Short comnu»nication Modification of Primary Amino Groups in Rat Heart Sarcolemma by 2,4,6-Trinitrobenzene Sulfonic Acid in aspect to the Activities of (Na

More information

MRP2 TR ATPase Assay Protocol CAT. NO. SBAT03

MRP2 TR ATPase Assay Protocol CAT. NO. SBAT03 MRP2 TR ATPase CAT. NO. SBAT03 Page 1 of 18 Determination of the interaction of drugs with the human MRP2 (ABCC2) transporter using the ATPase Assay For the following membrane products: SB-MRP2-Sf9-ATPase

More information

ENHANCEMENT BY F-ACTIN OF MGATP-DEPENDENT DOPAMINE UPTAKE INTO ISOLATED CHROMAFFIN GRANULES

ENHANCEMENT BY F-ACTIN OF MGATP-DEPENDENT DOPAMINE UPTAKE INTO ISOLATED CHROMAFFIN GRANULES Vol. 4, No. 1, September 1996 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 61-66 ENHANCEMENT BY F-ACTIN OF MGATP-DEPENDENT DOPAMINE UPTAKE INTO ISOLATED CHROMAFFIN GRANULES Kyoji Morita ~)*,

More information

Regulation of glycolysis/fructolysis in buffalo

Regulation of glycolysis/fructolysis in buffalo Regulation of glycolysis/fructolysis in buffalo spermatozoa K. K. Gandhi and S. R. Anand National Dairy Research Institute, Karnal-132001, India Summary. Assay of maximal activities of 11 glycolytic enzymes

More information

Glutathione Peroxidase Assay Kit

Glutathione Peroxidase Assay Kit Glutathione Peroxidase Assay Kit Catalog Number KA0882 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4

More information

Phospholipid Assay Kit

Phospholipid Assay Kit Phospholipid Assay Kit Catalog Number KA1635 100 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Kit for assay of thioredoxin

Kit for assay of thioredoxin FkTRX-02-V2 Kit for assay of thioredoxin The thioredoxin system is the major protein disulfide reductase in cells and comprises thioredoxin, thioredoxin reductase and NADPH (1). Thioredoxin systems are

More information

2. 2,4 Dinitro phenyl hydrazine (DNPH): I mm in 1N HCl. 5. Working standard: 1 in 20 dilution of the stock standard.

2. 2,4 Dinitro phenyl hydrazine (DNPH): I mm in 1N HCl. 5. Working standard: 1 in 20 dilution of the stock standard. -1 Estimation of Alanine Transaminase (ALT) (Mohun and Cook, 1957) Reagents I. Buffered substrate: [100 mm phosphate buffer, 200mM DL-alanine; 2 mm 2-oxo glutarate.}- Dissolved 1.5 g di potassium hydrogen

More information

Glutathione Synthesis in Human Erythrocytes

Glutathione Synthesis in Human Erythrocytes Glutathione Synthesis in Human Erythrocytes II. PURIFICATION AND PROPERTIES OF THE ENZYMES OF GLUTATHIONE BIOSYNTHESIS PHILI W. MAjEUS, M. J. BRAUNER, M. B. SMITH, and VIRGINIA MINNICH From the Departments

More information

The Pyruvate Carboxylase of Verticillium albo-atrum

The Pyruvate Carboxylase of Verticillium albo-atrum Journal of General Microbiology (I 974), 81, I 5- I 9 Printed in Great Britain The Pyruvate Carboxylase of Verticillium albo-atrum By R. E. HARTMAN Department of Biology, St Bonaventure University, St

More information

Data sheet. TBARS Assay kit. (Colorimetric/Fluorometric) Kit Contents. MDA-TBA Adduct. 2-Thiobarbituric Acid. Cat. No: CA995.

Data sheet. TBARS Assay kit. (Colorimetric/Fluorometric) Kit Contents. MDA-TBA Adduct. 2-Thiobarbituric Acid. Cat. No: CA995. Data sheet Cat. No: CA995 TBARS Assay kit (Colorimetric/Fluorometric) Introduction Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides.

More information

BIOL 347L Laboratory Three

BIOL 347L Laboratory Three Introduction BIOL 347L Laboratory Three Osmosis in potato and carrot samples Osmosis is the movement of water molecules through a selectively permeable membrane into a region of higher solute concentration,

More information

Alkaline Phosphatase Assay Kit

Alkaline Phosphatase Assay Kit Alkaline Phosphatase Assay Kit Catalog Number KA0817 500 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials

More information

Iodide transport in isolated cells of mouse submaxillary gland

Iodide transport in isolated cells of mouse submaxillary gland J. Biosci., Vol. 10, Number 3, September 1986, pp. 303 309. Printed in India. Iodide transport in isolated cells of mouse submaxillary gland R. K. BANERJEE*, A. K. BOSE, T. K. CHAKRABORTY, P. K. DE and

More information

BIOENERGETICS. 1. Detection of succinate dehydrogenase activity in liver homogenate using artificial electron acceptors.

BIOENERGETICS. 1. Detection of succinate dehydrogenase activity in liver homogenate using artificial electron acceptors. BIOENERGETICS Problems to be prepared: 1. Methods of enzymes activity assessment, the role of artificial electron acceptors and donors. 2. Reactions catalyzed by malate dehydrogenase, succinate dehydrogenase,

More information

TABLE OF CONTENTS GENERAL INFORMATION... 1

TABLE OF CONTENTS GENERAL INFORMATION... 1 BIOO RESEARCH PRODUCTS Glucose Assay Kit Manual Catalog #: 5611-01 BIOO Scientific Corp. 2011 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview... 1 Required Materials

More information

Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture

Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture Damage of embryo development caused by peroxidized and its association with albumin in culture Junko Otsuki, Ph.D., a,b Yasushi Nagai, M.D., a and Kazuyoshi Chiba, Ph.D. b a Nagai Clinic, Saitama; and

More information

GPx Equation 1 Æ R - O - H + GSSG + H2 O

GPx Equation 1 Æ R - O - H + GSSG + H2 O OXFORD BIOMEDICAL RESEARCH P.O. Box 522, Oxford MI 48371 USA USA: 800-692-4633 Fax: 248-852-4466 www.oxfordbiomed.com Colorimetric Assay for Cellular Glutathione Peroxidase Product No. FR 17 For Research

More information

Alkaline Phosphatase Assay Kit (Fluorometric)

Alkaline Phosphatase Assay Kit (Fluorometric) Alkaline Phosphatase Assay Kit (Fluorometric) Catalog Number KA0820 500 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...

More information

APPENDIX Heparin 2 mg heparin was dissolved in 0.9 % NaCl (10 ml). 200 µl of heparin was added to each 1 ml of blood to prevent coagulation.

APPENDIX Heparin 2 mg heparin was dissolved in 0.9 % NaCl (10 ml). 200 µl of heparin was added to each 1 ml of blood to prevent coagulation. APPENDIX 1 Preparation of reagents 1.1. Preparation of dosing solution Nonylphenol 15 mg of Nonylphenol was dissolved in olive oil (10 ml) and used as stock solution. The stock solution was serially diluted

More information

Name: Student Number

Name: Student Number UNIVERSITY OF GUELPH CHEM 454 ENZYMOLOGY Winter 2003 Quiz #1: February 13, 2003, 11:30 13:00 Instructor: Prof R. Merrill Instructions: Time allowed = 80 minutes. Total marks = 34. This quiz represents

More information

DIFFERENTIAL ph SENSITIVITY OF TISSUE SUPEROXIDE DISMUTASES

DIFFERENTIAL ph SENSITIVITY OF TISSUE SUPEROXIDE DISMUTASES Indian Journal of Clinical Biochemistry, 2006 / 21 (2) 129-133 DIFFERENTIAL ph SENSITIVITY OF TISSUE SUPEROXIDE DISMUTASES Samir P Patel and Surendra S Katyare Department of Biochemistry, Faculty of Science,

More information

TECHNICAL BULLETIN. R 2 GlcNAcβ1 4GlcNAcβ1 Asn

TECHNICAL BULLETIN. R 2 GlcNAcβ1 4GlcNAcβ1 Asn GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit Product Code PP0201 Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description Glycosylation is one of the most common posttranslational

More information

Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples:

Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples: Dr. Sanjeeva Srivastava IIT Bombay Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples: Sample preparation for serum proteome analysis Sample

More information

Protein Dephosphorylation Methods

Protein Dephosphorylation Methods Protein Dephosphorylation Methods Phosphospecific antibodies are designed to differentiate between the phosphorylated and the non-phosphorylated states of a protein. The method to determine if or how well

More information

Delta-9-tetrahydrocannabinol and human spermatozoa

Delta-9-tetrahydrocannabinol and human spermatozoa J. Biosci., Vol. 1, Number 3, September 1979, pp. 289 293. Printed in India. Delta-9-tetrahydrocannabinol and human spermatozoa INDIRA CHAKRAVARTY*, GIRISH SHAH**, ANIL R. SHETH** and JAGAT J. GHOSH *

More information

THE RESPIRATION MECHANISM OF PNEUMOCOCCUS. III*

THE RESPIRATION MECHANISM OF PNEUMOCOCCUS. III* THE RESPIRATION MECHANISM OF PNEUMOCOCCUS. III* BY M. G. SEVAG A~rD LORE MAIWEG (From the Robert Koch Institute, Berlin, Germany) (Received for publication, April 11, 1934) In two previous communications

More information

DELFIA Tb-N1 DTA Chelate & Terbium Standard

DELFIA Tb-N1 DTA Chelate & Terbium Standard AD0029P-1 (en) 1 DELFIA Tb-N1 DTA Chelate & AD0012 Terbium Standard For Research Use Only INTRODUCTION DELFIA Tb-N1 DTA Chelate is optimized for the terbium labeling of proteins and peptides for use in

More information

MEK1 Assay Kit 1 Catalog # Lot # 16875

MEK1 Assay Kit 1 Catalog # Lot # 16875 MEK1 Assay Kit 1 Kit Components Assay Dilution Buffer (ADB), Catalog # 20-108. Three vials, each containing 1.0ml of assay dilution buffer (20mM MOPS, ph 7.2, 25mM ß-glycerol phosphate, 5mM EGTA, 1mM sodium

More information

PDF hosted at the Radboud Repository of the Radboud University Nijmegen

PDF hosted at the Radboud Repository of the Radboud University Nijmegen PDF hosted at the Radboud Repository of the Radboud University Nijmegen The following full text is a publisher's version. For additional information about this publication click this link. http://hdl.handle.net/2066/142604

More information

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix Dorota Woźniak A, Andrzej Dryś B, and Adam Matkowski* A A Department of Pharmaceutical Biology

More information

THE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN

THE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN THE ESTIMATION OF TRYPSIN WITH HEMOGLOBIN BY M. L. ANSON Am) A. E. MIRSKY (From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller

More information

Enzymatic Assay of RIBONUCLEIC ACID POLYMERASE 1 (EC )

Enzymatic Assay of RIBONUCLEIC ACID POLYMERASE 1 (EC ) PRINCIPLE: Enzymatic Assay of RIBONUCLEIC ACID POLYMERASE 1 DNA + NTP RNA Polymerase > DNA + RNA + PP i PP i + UDPG UDPG Pyrophosphorylase > UTP + Glucose 1-Phosphate Glucose 1-Phosphate Phosphoglucomutase

More information

D-Mannitol Assay Kit (Colorimetric)

D-Mannitol Assay Kit (Colorimetric) D-Mannitol Assay Kit (Colorimetric) Catalog Number KA3760 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...

More information

Isolation and Identification of the Cytoplasmic Membrane

Isolation and Identification of the Cytoplasmic Membrane APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 1978, p. 851-856 Vol. 36, No. 6 0099-2240/78/0036-0851$02.00/0 Copyright C 1978 American Society for Microbiology Printed in U.S.A. Isolation and Identification

More information

potassium is included in the dialysis fluid. Unless the phosphate buffer

potassium is included in the dialysis fluid. Unless the phosphate buffer EFFECT OF POTASSIUM ON RAM SPERMATOZOA STUDIED BY A FLOW DIALYSIS TECHNIQUE H. M. DOTT and I. G. WHITE A.R.C. Unit of Reproductive Physiology and Biochemistry, University of Cambridge {Received 23rd July

More information

For the quantitative measurement of ATP Synthase Specific activity in samples from Human, Rat and Cow

For the quantitative measurement of ATP Synthase Specific activity in samples from Human, Rat and Cow ab109716 ATP Synthase Specific Activity Microplate Assay Kit Instructions for Use For the quantitative measurement of ATP Synthase Specific activity in samples from Human, Rat and Cow This product is for

More information

THE EFFECTS OF CHOLESTEROL DEPLETION ON THE SODIUM PUMP IN HUMAN RED CELLS

THE EFFECTS OF CHOLESTEROL DEPLETION ON THE SODIUM PUMP IN HUMAN RED CELLS Experimental Physiology (1991), 76, 437-443 Printed in Great Britain THE EFFECTS OF CHOLESTEROL DEPLETION ON THE SODIUM PUMP IN HUMAN RED CELLS F. J. LUCIO*, B. M. HENDRYt AND J. C. ELLORY* * University

More information

Pyruvate Kinase Is Protected by Glutathione-Dependent Redox Balance in Human Red Blood Cells Exposed to Reactive Oxygen Species

Pyruvate Kinase Is Protected by Glutathione-Dependent Redox Balance in Human Red Blood Cells Exposed to Reactive Oxygen Species October 2008 Biol. Pharm. Bull. 31(10) 1875 1881 (2008) 1875 Pyruvate Kinase Is Protected by Glutathione-Dependent Redox Balance in Human Red Blood Cells Exposed to Reactive Oxygen Species Yuki OGASAWARA,*

More information

Monoamine oxidase in sympathetic nerves: a transmitter specific enzyme type

Monoamine oxidase in sympathetic nerves: a transmitter specific enzyme type Br. J. Pharmac. (1971), 43, 814-818. Monoamine oxidase in sympathetic nerves: a transmitter specific enzyme type C. GORIDIS AND N. H. NEFF Laboratory of Preclinical Pharmacology, National Institute of

More information

TECHNICAL BULLETIN. Sialic Acid Quantitation Kit. Catalog Number SIALICQ Storage Temperature 2 8 C

TECHNICAL BULLETIN. Sialic Acid Quantitation Kit. Catalog Number SIALICQ Storage Temperature 2 8 C Sialic Acid Quantitation Kit Catalog Number SIALICQ Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total

More information

Kinin forming and destroying activities in human bile and mucous membranes of the biliary tract

Kinin forming and destroying activities in human bile and mucous membranes of the biliary tract Br. J. Pharmac. (1969), 37, 172-177. Kinin forming and destroying activities in human bile and mucous membranes of the biliary tract H. M. NIELSEN Surgical Department L, Aarhus Municipal Hospital, University

More information

Serrata) Alkaline Phosphatase

Serrata) Alkaline Phosphatase Vol. 41, No. 5, April 1997 BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL Pages 951-959 An Essential Tryptophan Residue of Green Crab (Syclla Serrata) Alkaline Phosphatase Wen-Zhu Zheng 1, Qing-Xi Chen

More information

A GLUCOSEPHOSPHATE ISOMERASE INHIBITOR OF SEASONAL OCCURRENCE IN COD (GADUS MORHUA) AND OTHER FISH

A GLUCOSEPHOSPHATE ISOMERASE INHIBITOR OF SEASONAL OCCURRENCE IN COD (GADUS MORHUA) AND OTHER FISH J. mar. biol. Ass. U.K. (969) 49, 447-453 447 Printed in Great Britain A GLUCOSEPHOSPHATE ISOMERASE INHIBITOR OF SEASONAL OCCURRENCE IN COD (GADUS MORHUA) AND OTHER FISH By P. R. DANDO The Plymouth Laboratory

More information

20X Buffer (Tube1) 96-well microplate (12 strips) 1

20X Buffer (Tube1) 96-well microplate (12 strips) 1 PROTOCOL MitoProfile Rapid Microplate Assay Kit for PDH Activity and Quantity (Combines Kit MSP18 & MSP19) 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 MSP20 Rev.1 DESCRIPTION MitoProfile Rapid Microplate

More information

User s Manual and Instructions

User s Manual and Instructions User s Manual and Instructions Mitochondria Activity Assay (Cytochrome C Oxidase Activity Assay) Kit Catalog Number: KC310100 Introduction Mitochondria are the eukaryotic subcellular organelles that contain

More information

Reconstitution of Neutral Amino Acid Transport From Partially Purified Membrane Components From Ehrlich Ascites Tumor Cells

Reconstitution of Neutral Amino Acid Transport From Partially Purified Membrane Components From Ehrlich Ascites Tumor Cells Journal of Supramolecular Structure 7:481-487 (1977) Molecular Aspects of Membrane Transport 5 1 1-5 17 Reconstitution of Neutral Amino Acid Transport From Partially Purified Membrane Components From Ehrlich

More information

OxiSelect Human Oxidized LDL ELISA Kit (MDA- LDL Quantitation)

OxiSelect Human Oxidized LDL ELISA Kit (MDA- LDL Quantitation) Product Manual OxiSelect Human Oxidized LDL ELISA Kit (MDA- LDL Quantitation) Catalog Number STA- 369 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are

More information

Status of LDL Oxidation and antioxidant potential of LDL in Type II Diabetes Mellitus

Status of LDL Oxidation and antioxidant potential of LDL in Type II Diabetes Mellitus Biomedical Research 2010; 21 (4): 416-418 Status of LDL Oxidation and antioxidant potential of LDL in Type II Singh N, Singh N, Singh S K, Singh A K, Bhargava V. Department of Biochemistry, G. R. Medical

More information

Phospholipase D Activity of Gram-Negative Bacteria

Phospholipase D Activity of Gram-Negative Bacteria JOURNAL OF BACTERIOLOGY, Dec. 1975, p. 1148-1152 Copyright 1975 American Society for Microbiology Vol. 124, No. 3 Printed in U.S.A. Phospholipase D Activity of Gram-Negative Bacteria R. COLE AND P. PROULX*

More information

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 62, No. 2, 19Z5 L-KYNURENINE AMINOTRANSFERASE AND L-~-AMINOADIPATE AMINOTRANSFERASE. I. EVIDENCE FOR IDENTITY Michael C. Tobes and Merle Mason Department of Biological Chemistry, The University of

More information

OxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation)

OxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation) Product Manual OxiSelect Human Oxidized LDL ELISA Kit (OxPL-LDL Quantitation) Catalog Number STA-358 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are submicroscopic

More information

Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit

Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit PROTOCOL Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit DESCRIPTION Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit Sufficient materials

More information

Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard. Product Number: AD0014

Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard. Product Number: AD0014 TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard Product Number: AD0014 INTRODUCTION: Iodoacetamido-activated

More information

BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL. Xin-Min Li ~, Jia-Zuan Ni 1, Jian-Wen Chen 2 and Fen Hwang 2

BIOCHEMISTRY and MOLECULAR BIOLOGY INTERNATIONAL. Xin-Min Li ~, Jia-Zuan Ni 1, Jian-Wen Chen 2 and Fen Hwang 2 Vol. 45, No. 2, June 1998 Pages 323-330 EFFECTS OF La 3 ON LIPID FLUIDITY AND STRUCTURAL TRANSITIONS IN HUMAN ERYTHROCYTE MEMBRANES Xin-Min Li ~, Jia-Zuan Ni 1, Jian-Wen Chen 2 and Fen Hwang 2 1Laboratory

More information

PhosFree TM Phosphate Assay Biochem Kit

PhosFree TM Phosphate Assay Biochem Kit PhosFree TM Phosphate Assay Biochem Kit (Cat. # BK050) ORDERING INFORMATION To order by phone: (303) - 322-2254 To order by Fax: (303) - 322-2257 To order by e-mail: cservice@cytoskeleton.com Technical

More information

TBARS Assay Kit Catalog Number:

TBARS Assay Kit Catalog Number: TBARS Assay Kit : 0801192 INTENDED USE The sensitivity of measuring Thiobarbituric Acid Reactive Substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation,

More information

Available online through ISSN

Available online through  ISSN Research Article Available online through www.ijrap.net ISSN 2229-3566 PROTECTIVE ROLE OF GLUTATHIONE IN TUNGSTEN-INDUCED TOXICITY IN BLOOD COMPONENTS: A PHARMACOLOGICAL AND TOXICOLOGICAL PERSPECTIVE Arshad

More information

Lipid Concentration And The Extent Of Their Peroxidation In Nigerian Hypertensives

Lipid Concentration And The Extent Of Their Peroxidation In Nigerian Hypertensives ISPUB.COM The Internet Journal of Health Volume 8 Number 1 Lipid Concentration And The Extent Of Their Peroxidation In Nigerian Hypertensives P Igbinaduwa, B Igbinaduwa, I Oforofuo Citation P Igbinaduwa,

More information

1.2 Systematic Name: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

1.2 Systematic Name: Orthophosphoric-monoester phosphohydrolase (alkaline optimum) Document Title Alkaline Phosphatase Page 1 of 6 Originating Department QA Approval Departments QA, QC Approval Date 5 th June 2017 Effective Date 8 th June 2017 1.0 PRODUCT DETAILS 1.1 Enzyme Name: Alkaline

More information

RUBISCO > 2 moles of 3-phosphoglycerate Mg +2

RUBISCO > 2 moles of 3-phosphoglycerate Mg +2 PRINCIPLE: RuDP + CO 2 RUBISCO > 2 moles of 3-phosphoglycerate Mg +2 3-Phosphoglycerate + ATP PGK > Glycerate 1,3-Diphosphate + ADP Glycerate 1,3-Diphosphate + ß-NADH GAPDH > Glyceraldehyde 3-Phosphate

More information

and Its Inhibitor Human-Polymorphonuclear-Leucocyte Neutral Protease Studies with Fluorescein-Labelled Polymeric Collagen Fibrils as a Substrate

and Its Inhibitor Human-Polymorphonuclear-Leucocyte Neutral Protease Studies with Fluorescein-Labelled Polymeric Collagen Fibrils as a Substrate Eur. J. Biochem. 67, 165169 (1976) HumanPolymorphonuclearLeucocyte Neutral Protease and Its Inhibitor Studies with FluoresceinLabelled Polymeric Collagen Fibrils as a Substrate Frank S. STEVEN, David W.

More information

Total Phosphatidic Acid Assay Kit

Total Phosphatidic Acid Assay Kit Product Manual Total Phosphatidic Acid Assay Kit Catalog Number MET- 5019 100 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Phosphatidic Acid (PA) is a critical precursor

More information

DELFIA Eu-DTPA ITC Chelate & Europium Standard

DELFIA Eu-DTPA ITC Chelate & Europium Standard AD0026P-3 (en) 1 DELFIA Eu-DTPA ITC Chelate & AD0021 Europium Standard For Research Use Only INTRODUCTION DELFIA Eu-DTPA ITC Chelate is optimized for the europium labelling of proteins and peptides for

More information

Lipid Peroxide Formation in Microsomes

Lipid Peroxide Formation in Microsomes Biochem. J. (1969) 113, 35 Printed in Great Britain 35 Lipid Peroxide Formation in Microsomes THE ROLE OF NON-HAEM IRON By E. D. WILLS Department of Biochemi8try, Medical College of St Bartholomew'8 Ho8pital,

More information

I mutants accumulate pyruvate when growing in the presence of isoleucine and

I mutants accumulate pyruvate when growing in the presence of isoleucine and THE iv-3 MUTANTS OF NEUROSPORA CRASSA 11. ACTIVITY OF ACETOHYDROXY ACID SYNTHETASE DINA F. CAROLINE, ROY W. HARDINGZ, HOMARE KUWANA3, T. SATYANARAYANA AND R.P. WAGNER4 Genetics Foundation, The University

More information

EFFECTS OF FLUORIDE ON LIPID PEROXIDATION IN RABBITS

EFFECTS OF FLUORIDE ON LIPID PEROXIDATION IN RABBITS :185 189 Research report 185 EFFECTS OF FLUORIDE ON LIPID PEROXIDATION IN RABBITS M Akdogan, a G Eraslan, b F Gultekin, a F Sahindokuyucu, c D Essiz d Ankara and Isparta, Turkey SUMMARY: Twenty-one 6-month-old

More information

ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric)

ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric) Version 10 Last updated 19 December 2017 ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric) For the measurement of triglycerides in various samples. This product is for research

More information

Biodegradative Threonine Dehydratase. Reduction of Ferricyanide by an Intermediate of the Enzyme-Catalyzed Reaction

Biodegradative Threonine Dehydratase. Reduction of Ferricyanide by an Intermediate of the Enzyme-Catalyzed Reaction Eur. J. Biochem. Y I, 527-532 (1978) Biodegradative Threonine Dehydratase. Reduction of Ferricyanide by an Intermediate of the Enzyme-Catalyzed Reaction Prasanta DATTA and Ranjan BHADRA Department of Biological

More information

BIOO LIFE SCIENCE PRODUCTS

BIOO LIFE SCIENCE PRODUCTS BIOO LIFE SCIENCE PRODUCTS FOR REFERENCE PURPOSES This manual is for Reference Purposes Only. DO NOT use this protocol to run your assays. Periodically, optimizations and revisions are made to the kit

More information

(Adams 8c Purves 1958), or LATS-protector (LATS-P) (Adams 8c Kennedy. 1967). The failure of the McKenzie (1958) mouse bioassay to detect LATS in

(Adams 8c Purves 1958), or LATS-protector (LATS-P) (Adams 8c Kennedy. 1967). The failure of the McKenzie (1958) mouse bioassay to detect LATS in Department of Endocrinology, Royal Prince Alfred Hospital, and Department of Medicine, University of Sydney, Sydney, Australia THE THYROTROPHIN RECEPTOR IN HUMAN THYROID PLASMA MEMBRANES: EFFECT OF SERUM

More information

Cell Lysis Buffer. Catalog number: AR0103

Cell Lysis Buffer. Catalog number: AR0103 Cell Lysis Buffer Catalog number: AR0103 Boster s Cell Lysis Buffer is a ready-to-use Western blot related reagent solution used for efficient extraction of total soluble protein in nondenatured state

More information

Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard. Product Number: AD0013

Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard. Product Number: AD0013 TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard Product Number: AD0013 INTRODUCTION: Fluorescent isothiocyanato-activated

More information

DAG (Diacylglycerol) Assay Kit

DAG (Diacylglycerol) Assay Kit Product Manual DAG (Diacylglycerol) Assay Kit Catalog Number MET-5028 100 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Diacylglycerols (DAG) are key intermediates in the

More information

Separation of Plasma and Serum and Their Proteins from Whole Blood

Separation of Plasma and Serum and Their Proteins from Whole Blood Separation of Plasma and Serum and Their Proteins from Whole Blood BCH 471 [Practical] BLOOD COMPOSITION Other names to blood cells Red blood cells (erythrocytes) White blood cells (leukocytes) Platelets

More information

INHIBITION OF TRYPSIN AND PAPAIN BY SODIUM AUROTHIOMALATE MEDIATED BY EXCHANGE REACTIONS

INHIBITION OF TRYPSIN AND PAPAIN BY SODIUM AUROTHIOMALATE MEDIATED BY EXCHANGE REACTIONS Br. J. Pharmac. (1982), 75, 333-339 INHIBITION OF TRYPSIN AND PAPAIN BY SODIUM AUROTHIOMALATE MEDIATED BY EXCHANGE REACTIONS M.M. GRIFFIN & F.S. STEVEN Department of Medical Biochemistry, University of

More information

OXIDATIVE STRESS STUDIES ON LIPID MODEL MEMBRANES

OXIDATIVE STRESS STUDIES ON LIPID MODEL MEMBRANES OXIDATIVE STRESS STUDIES ON LIPID MODEL MEMBRANES MARCELA ELISABETA BARBINTA-PATRASCU *, LAURA TUGULEA * * Faculty of Physics, University of Bucharest, Romania Received December 21, 2004 The liposomes

More information

OCR (A) Biology A-level

OCR (A) Biology A-level OCR (A) Biology A-level Topic 2.2: Biological molecules Notes Water Water is a very important molecule which is a major component of cells, for instance: Water is a polar molecule due to uneven distribution

More information

THE EFFECT OF TITANIUM ON THE OXIDATION OF SULFHYDRYL GROUPS BY VARIOUS TISSUES

THE EFFECT OF TITANIUM ON THE OXIDATION OF SULFHYDRYL GROUPS BY VARIOUS TISSUES THE EFFECT OF TITANIUM ON THE OXIDATION OF SULFHYDRYL GROUPS BY VARIOUS TISSUES BY FREDERICK BERNHEIM AND MARY L. C. BERNHEIM (From the Departments oj Physiology and Pharmacology and Biochemistry, Duke

More information

Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General

Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General For the detection and quantitation of human OxLDL in plasma, serum or other biological fluid samples Cat. No. KT-959 For Research Use Only.

More information