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1 DEPARTMENT OF SCIENCE COURSE OUTLINE Fall 2017 BC 2000 INTRODUCTORY BIOCHEMISTRY 3 (3-0-0) 45 HOURS FOR 15 WEEKS INSTRUCTOR: Philip Johnson PHONE: OFFICE: J224 PJohnson@gprc.ab.ca OFFICE HOURS: Tuesdays hrs & hrs Thursdays hrs Fridays hrs PREREQUISITE(S)/COREQUISITE: CH 1010 and CH 1610 or CH 2610 REQUIRED TEXT/RESOURCE MATERIALS: Essential Biochemistry (4 th Edition, 2018 or 3 rd Edition, 2014) Charlotte W. Pratt and Kathleen Cornely John Wiley & Sons Inc. Publishers SUPPLEMENTS: Practice quizzes will be made available on Moodle to aid preparation for exams. These can be completed at any time by students. A link to the textbook website is also available on Moodle. This site provides access to a number of useful resources. CALENDAR DESCRIPTION: An introduction to the fundamental principles of biochemistry. Protein structure and function; lipids and the structure of biological membranes; nucleotides and the structure of nucleic acids; bioenergetics and the metabolism of carbohydrates, lipids and nitrogen; the integration and regulation of cellular metabolism. This course is designed both for students who require a single term course in the fundamental principles of biochemistry, AND for students who intend to take further courses in biochemistry. Copyright 2009, Grande Prairie Regional College and its licensors. 1

2 CREDIT/CONTACT HOURS: 3 credits (3-0-0) DELIVERY MODE(S): Classes Monday (J201) Friday COURSE OBJECTIVES: 1. To gain an understanding of biochemical reactions and pathways. 2. To develop critical thinking skills with respect to biochemistry (J201) LEARNING OUTCOMES: 1. Students should know the reactions of common metabolic pathways. 2. Students should understand how metabolic pathways are integrated. 3. Students should understand the relationship between metabolism and disease. TRANSFERABILITY: University of Alberta (Biochemistry 200) University of Calgary (Biochemistry 341) EVALUATIONS: Mid-term Exam I 30% Mid-term Exam II 30% Final Exam 40% GRADING CRITERIA: GRANDE PRAIRIE REGIONAL COLLEGE GRADING CONVERSION CHART Alpha Grade 4-point Percentage Designation A A EXCELLENT A B FIRST CLASS STANDING B B GOOD C C SATISFACTORY C D D MINIMAL PASS F FAIL WF FAIL, withdrawal after the deadline ** Grade of D or D+ may not be acceptable for transfer to other post-secondary institutions. Students are cautioned that it is their responsibility to contact the receiving institutions to ensure transferability Copyright 2009, Grande Prairie Regional College and its licensors. 2

3 BC2000 Fall Topic Outline &Text Readings Topic 3 rd Edition pages 4 th Edition pages Biological Molecules Types of biomolecules Biopolymers Nucleosides and nucleotides Basic structure of DNA and RNA Functions of Nucleic acids (Central Dogma) Protein Structure and Function Overview Amino acids Peptide bonds and primary structure Secondary structures Tertiary structure and stabilization Protein folding & Quarternary structure Oxygen binding to myoglobin and haemoglobin Lipids and Biological Membranes Fatty acids, triacylglycerols and membrane lipids Lipid bilayers and membrane fluidity Membrane proteins Fluid Mosaic Model Passive & Active membrane transport Enzymes What is an enzyme? Classifying enzymes Co-enzymes and dietary vitamins 54-55; ; Catalytic mechanisms Substrate binding Enzyme kinetics Enzyme inhibition Allosteric enzymes Copyright 2009, Grande Prairie Regional College and its licensors. 3

4 Other in vivo regulatory mechanisms Co-enzymes and roles as electron carriers Introduction to Metabolism Energy and metabolism Food and Fuel Storage and use of fuels Metabolic pathways and common intermediates Oxidation and reduction Overview of metabolism Free energy changes in metabolic reactions Energy currency, ATP, coupled reactions Fig 3-3a Fig 3-2a Glucose Metabolism Introduction , ; 349; 329 Glycolysis Fates of Pyruvate Anaerobic exercise and the Cori Cycle Gluconeogenesis and Glycogen metabolism Pentose phosphate pathway Summary of glucose metabolism ; ; ; ; Hormonal regulation Citric Acid Cycle and Oxidative Phosphorylation Introduction Conversion of pyruvate to acetyl-coa Reactions of the Citric Acid Cycle Regulation of the Citric Acid Cycle Catabolism, anabolism and anapleurotic reactions Overview of oxidative phosphorylation Mitochondria and Electron transport chain Chemiosmosis ATP synthase ATP yield from aerobic catabolism of glucose Copyright 2009, Grande Prairie Regional College and its licensors. 4

5 Metabolism of Fats, Fatty Acids and Cholesterol Overview of fat metabolism Fig Fig Transport of lipids TAG synthesis Lipases and TAG breakdown Degradation of fatty acids (activation & transport) Degradation of fatty acids (β-oxidation) Glyoxylate cycle Fatty acid synthesis Regulation of fatty acid metabolism Fat metabolism and diabetes Ketone bodies and ketogenesis Cholesterol synthesis and regulation Fates of cholesterol Good and Bad cholesterol 458 Box 17A Box 17A Nitrogen Metabolism Nitrogen fixation and assimilation Transamination and amino acid synthesis Catabolism of amino acids The Urea Cycle and nitrogen disposal STUDENT RESPONSIBILITIES: Students are expected to attend all classes, seminars and laboratory sessions. All assignments must be completed in full and handed in by the date specified. Refer to the College Policy on Student Rights and Responsibilities at STATEMENT ON PLAGIARISM AND CHEATING: Cheating and plagiarism will not be tolerated and there will be penalties. For a more precise definition of plagiarism and its consequences, refer to the Student Conduct section of the College Admission Guide at or the College Policy on Student Misconduct: Plagiarism and Cheating at **Note: all Academic and Administrative policies are available on the same page. Copyright 2009, Grande Prairie Regional College and its licensors. 5

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