ANALYSIS OF CARNAUBA WAX ON BPX5
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1 FOO 01 - FOOD ANALYSIS OF CARNAUBA WAX ON BPX5 CARNAUBA WAX Column Part No.: Phase: BPX5, 0.25 µm film 12 m x 0.32 mm ID Initial Temp: 100 C, 2 min Rate: 10 C/min Final Temp: Detector: Sensitivity: Injection Mode: 350 C, 20 min FID 40 x AFS On Column H2, 10 psi. Notes: The high temperature capability of the BPX5 made this analysis reproducible. On-column injection is recommended to reduce discrimination of high molecular weight compounds. 1. C26 alkanol 2. C28 alkanol 3. C30 alkanol 4. C32 alkanol 5. C46 ester 6. C48 ester 7. C50 ester 8. C52 ester 9. C54 ester 10. C56 ester 11. C60 ester 12. C62 ester 13. C64 ester 15. C66 ester FOO 01 Copyright SGE Analytical Science Pty Ltd July 2008
2 FOO 02 - FOOD ANALYSIS OF FOOD ADDITIVES ANTIMICROBIALS ON BP5 FOOD ADDITIVES - ANTIMICROBIALS Column Part No.: Phase: BP5, 0.5 µm film 25 m x 0.32 mm ID Initial Temp: 160 C, 0 min Rate: 15 C/min Final Temp: Detector: Sensitivity: Injection Mode: 280 C, 0 min FID 256 x AFS Split 1. Methyl Paraben 2. Ethyl Paraben 3. Propyl Paraben 4. Butyl Paraben 5. Heptyl Paraben FOO 02 Copyright SGE Analytical Science Pty Ltd July 2008
3 FOO 03 - FOOD ANALYSIS OF SCOTCH WHISKY ON BP20 SCOTCH WHISKY Column Part No.: Phase: BP20, 1.0 µm film 12 m x 0.53 mm ID Initial Temp: 55 C, 3 min Rate: 10 C/min Final Temp: Detector: Sensitivity: Injection Mode: 120 C, 0 min FID 128 x AFS Split 1. Acetaldehyde 2. Ethyl Acetate 3. Methanol 4. Ethanol 5. Propan-1-ol 6. 2-Methylpropan-1-ol 7. 2-Methylbutan-1-ol + 3-Methylbutan-1-ol FOO 03 Copyright SGE Analytical Science Pty Ltd July 2008
4 FOO 04 - FOOD ANALYSIS OF 13 SUGAR COMPONENT ALDITOL ACETATE MIXTURE ON BPX70 13 Component Alditol Acetate Mixture Column Part No.: Phase: BPX70, 0.25 µm film 30 m x 0.25 mm I.D. Initial Temp.: 190 C, 1 min. Program Rate: 3 C/min. Final Temp: 260 C, 10min. He, 50 kpa Detector: MS (Electron Impact Ionisation, Ionisation Potential 70 ev) Scan 100 m/z to 350 m/z in 0.3 s. Injection Mode: Split 50:1 Notes: BPX70 column, though a very polar phase, is compatible with GC/MS systems 1. Erythritol 2. 2-Deoxy-ribitol 3. Rhamnitol 4. Fucitol 5. Ribitol 6. Arabinitol 7. Xylitol 8. 2-Deoxy-glucitol 9. Allitol 10. Mannitol 11. Galacitol 12. Glucitol 13. Myo-inositol FOO 04 Copyright SGE Analytical Science Pty Ltd July 2008
5 FOO 05 - FOOD ARABINOGALACTAN PROTEIN FROM PLANT CELL SUSPENSION CULTURE ON BPX70 ARABINOGALACTAN PROTEIN FROM PLANT CELL SUSPENSION CULTURE Column Part No.: Phase: BPX70, 0.25 µm film 30 m x 0.25 mm I.D. Initial Temp.: 190 C, 1 min. Program Rate: 3 C/min. Final Temp: Detector: Injection Mode: 260 C, 10min. He, 50 kpa HP5790MSD. Split Notes: BPX70 is the only polar column commercially available suitable for GC/MS application 1. 2,3,4,6-O-methyl glucitol 2. 2,4,6-O- methyl glucitol 3. 3,4,6-O-methyl glucitol 4. 2,3,4-O-methyl glucitol 5. 2,3,6-O-methyl glucitol 6. 2,6-O-methyl glucitol 7. 4,6-O-methyl glucitol 8. 3,6-O-methyl glucitol 9. 2,4-O-methyl glucitol 10. 2,3-O-methyl glucitol 11. 3,4-O-methyl glucitol O-methyl glucitol O-methyl glucitol O-methyl glucitol O-methyl glucitol 16. glucitol hexa-acetate 17. myo-inositol hexa-acetate FOO 05 Copyright SGE Analytical Science Pty Ltd July 2008
6 FOO 06 - FOOD ANALYSIS OF SUGAR ALDITOL ACETATES ON A 12M BPX70 COLUMN ALDITOL ACETATES Column Part No.: Phase: BPX70, 0.25 µm film 12 m x 0.32 mm I.D. Temp.: Isothermal at 210 C H 2 Detector: F.I.D. Sensitivity: 16 x AFS Injection Mode: Split, 50:1 Notes: The polarity of BPX70 enables rapid analysis of Alditol Acetates. BPX70 is the only highly polar column compatible with GC/MS 1. Erythritol 2. Deoxyribitol 3. Rhamnitol 4. Fucitol 5. Ribitol 6. Arabinitol 7. Xylitol 8. Deoxyglucitol 9. Allitol 10. Mannitol 11. Galactitol 12. Glucitol 13. Inositol FOO 06 Copyright SGE Analytical Science Pty Ltd July 2008
7 FOO 07 - FOOD ANALYSIS OF MONO, DI, & TRIGLYCERIDES ON BPX5 ANALYSIS OF MONO, Di & TRIGLYCERIDES Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.32 mm I.D. Initial Temp.: 80 C, 1 min Rate: Final Temp.: Detector: Injector Mode: Carrier: 10 C/min 365 C, 10 min FID On-Column (SGE OCI-5) He, 10 psi Notes: BPX5 column allows this analysis to be performed on a routine basis. On-column injection is recommended to ensure no loss of high MW fraction. FOO 07 Copyright SGE Analytical Science Pty Ltd July 2008
8 FOO 08 - FOOD TRIGLYCERIDE DISTRIBUTION IN MILK FAT ON BPX5 TRIGLYCERIDE DISTRIBUTION IN MILK FAT Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.53 mm I.D. Initial Temp.: 100 C, 0.5 min. Rate 1: 50 C/min. Temp.: 280 C Rate 2: 10 C/min. Final Temp: 360 C, 5 min. He, 6 psi Injection Mode: On-column (OCI-5) Injection Volume: 1 µl PEAK TRIGLYCERIDE COMPOSITION No. CARBON No. (%) 1 T T T T T T T T T T T T T T T T T FOO 08 Copyright SGE Analytical Science Pty Ltd July 2008
9 FOO 09 - FOOD ANALYSIS OF TRIGLYCERIDE DISTRIBUTION IN LARD ON BPX5 TRIGLYCERIDE DISTRIBUTION IN LARD Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.53 mm I.D. Initial Temp.: 100 C, 0.5 min. Rate 1: 50 C/min. Notes: Recommended Operating Conditions Carrier Gas Velocity (Helium): cm/sec approx., Pressure: 5-7 psi Sample Concentration Temp.: 280 C Rate 2: 10 C/min. Final Temp: 360 C, 5 min. He, 6 psi Injection Mode: On-column (OCI-5) Injection Volume: 1 µl On-column: - Olive oil, lard and cocoa butter : mg/ml ( % solution) Injection Temp.: 100 C, solution dissolved in iso-octane Peak TRIGLYCERIDE COMPOSITION No. CARBON No. (%) 1 T T T T T T T FOO 09 Copyright SGE Analytical Science Pty Ltd July 2008
10 FOO 10 - FOOD STANDARD MIXTURE OF TRIGLYCERIDES ON BPX5 STANDARD MIXTURE OF TRIGLYCERIDES Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.53 mm I.D. Initial Temp.: 100 C, 0.5 min. Rate 1: 50 C/min. Temp.: 280 C Rate 2: 10 C/min. Final Temp: 360 C, 5 min. He, 6 psi Injection Mode: On-column (OCI-5) Injection Volume: 1 µl Notes: This analysis requires on-column injection to provide quantitative results Peak TRIGLYCERIDE No. CARBON No. 1 T36 2 T38 3 T40 4 T42 5 T44 6 T46 7 T48 8 T50 9 T52 10 T54 FOO 10 Copyright SGE Analytical Science Pty Ltd July 2008
11 FOO 11 - FOOD ANALYSIS OF TRIGLYCERIDE DISTRIBUTION IN COCOA BUTTER ON BPX5 Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.53 mm I.D. Initial Temp.: 100 C, 0.5 min Rate 1: 50 C/min Temp: 280 C Rate 2: 10 C/min Final Temp.: 360 C, 5 min Detector: FID Injector Mode: On-Column (SGE OCI-5) Carrier: He, 6 psi Injection Volume: 1 µl Notes: Recommended Operating Conditions Carrier Gas Velocity (Helium): cm/sec approx., Pressure: 5-7 psi Sample Concentration On-column: - cocoa butter: mg/ml ( % solution) Injection Temp.: 100 C, solution dissolved in iso-octane Peak TRIGLYCERIDE COMPOSITION No. CARBON No. (%) 1 T T T T T T FOO 11 Copyright SGE Analytical Science Pty Ltd July 2008
12 FOO 12 - FOOD TRIGLYCERIDE DISTRIBUTION IN OLIVE OIL ON BPX5 Column Part No.: Phase: BPX5, 0.25 µm 12 m x 0.53 mm I.D. Initial Temp.: 100 C, 0.5 min Rate 1: 50 C/min Temp: 280 C Notes: Recommended Operating Conditions Carrier Gas Velocity (Helium): cm/sec approx., Pressure: 5-7 psi Sample Concentration Rate 2: 10 C/min Final Temp.: 360 C, 5 min Detector: FID Injector Mode: On-Column (SGE OCI-5) Carrier: He, 6 psi Injection Volume: 1µL On-column: - Olive oil : mg/ml ( % solution) Injection Temp.: 100 C, solution dissolved in iso-octane Peak TRIGLYCERIDE COMPOSITION No. CARBON No. (%) 1 T T T T T T FOO 12 Copyright SGE Analytical Science Pty Ltd July 2008
13 FOO 13 - FOOD ANALYSIS OF PARTIALLY METHYLATED GLUCOSE ON BPX70 PARTIALLY METHYLATED GLUCOSE Column Part No.: Phase: BPX70, 0.25 µm film 25 m x 0.22 mm I.D. Initial Temp.: 185 C, 1min. Program Rate: 3 C/min. Final Temp: Detector: Injection Mode: 260 C, 10min. He, 50 kpa MS (Electron Impact Ionisation, Ionisation Potential 70eV) Scan 100m/z to 350 m/z in 0.3s. Split Notes: BPX70 is compatible with GC/MS systems. FOO 13 Copyright SGE Analytical Science Pty Ltd July ,3,4-O-methyl rhamnitol 2. 2,3,5-O- methyl arabinitol (f) 3. 2,3,4-O-methyl arabinitol (p) 4. 3,5-O-methyl arabinitol 5. 2,3,4,6-O-methyl glucitol 6. 2,5-O-methyl arabinitol 7. 2,3,4,6-O-methyl galactitol 8. 2,3-O-methyl arabinitol 9. 2,4,6-O-methyl galactitol O-methyl arabinitol 11. 2,3,6-O-methyl glucitol 12. 2,3,4-O-methyl galactitol 13. arabinitol hexa-acetate 14. xylitol hexa-acetate 15. 2,3,4,6-O-methyl galactitol O-methyl galactitol O-methyl galactitol 18. galactitol hexa-acetate 19. glucitol hexa-acetate 20. myo-inositol hexa-acetate
14 FOO 14 - FOOD ANALYSIS OF BUTTER FAT ON HT5 ANALYSIS OF BUTTER FAT Column Part No.: Phase: HT5, 0.1 µm 6 m x 0.53 mm I.D. (Aluminum Clad) Initial Temp.: 200 C, 0 min Program Rate: Final Temp.: Detector: Sensitivity: Injection Mode: 10 C/min 370 C, 5 min H 2, 2 psi F.I.D. 32 x AFS On-column FOO 14 Copyright SGE Analytical Science Pty Ltd July 2008
15 FOO 15 - FOOD FREE BASE AND METAL COMPLEXED PORPHYRINS ANALYSIS ON HT5 FREE BASE AND METAL COMPLEXED PORPHYRINS Column Part No.: Phase: HT5, 0.1 µm 12 m x 0.32 mm I.D. (Aluminium Clad) Initial Temp.: 60 C, 0 min Notes: The high temperature nature of HT5 makes it the best column available for this analysis Program Rate 1: 20 C/min to 300 C Program Rate 2: 10 C/min Final Temp.: 430 C, 5 min H 2, 15 psi Detector: F.I.D. Sensitivity: 16 x AFS Injection Mode: On-column 1. Porphyrin M=TiO 2. Porphyrin M=Cu 3. Porphyrin M=VO 4. Porphyrin M=Pd FOO 15 Copyright SGE Analytical Science Pty Ltd July 2008
16 FOO 16 - FOOD ANALYSIS OF TRIGLYCERIDE STANDARDS ON HT5 ANALYSIS OF TRIGLYCERIDE STANDARDS Column Part No.: Phase: HT5, 0.1 µm 6 m x 0.53 mm I.D. (Aluminium Clad) Initial Temp.: 60 C, 0 min Program Rate: 10 C/min Final Temp.: Detector: Sensitivity: Injection Mode: 370 C, 5 min H 2, 2 psi F.I.D. 32 x AFS On-column Notes: For the analysis of triglycerides, on-column injection is recommended. Temperatures above 380 C are not recommended as triglycerides can degrade. FOO 16 Copyright SGE Analytical Science Pty Ltd July 2008
17 FOO 17 - FOOD THE ANALYSIS OF PLANT STEROLS ISOTHERMALLY ON BPX35 Column Part No.: Phase: BPX35, 0.25 µm 30 m x 0.22 mm ID Temp.: 310 C Detector: FID, 360 C Injector Mode: Split, 100:1 He, 20 psi Injection Volume: 1 µl 1. Caprosterol 2. Cholesterol 3. Cholestanol 4. Brassicasterol 5. Desmosterol 6. Ergosterol 7. Campasterol 8. Stigmasterol 9. Unknown 10. beta-sitosterol 11. Lanosterol FOO 17 Copyright SGE Analytical Science Pty Ltd July 2008
18 FOO 18 - FOOD THE ANALYSIS OF PLANT STEROLS WITH TEMPERATURE PROGRAM ON BPX35 Column Part No.: Phase: BPX35, 0.25 µm 30 m x 0.25 mm ID Initial Temp.: 280 C, 1 min Rate 1: 4 C/min Final Temp.: 350, 5 min Detector: FID, 360 C Injector Mode: Split, 100:1 He, 20 psi Injection Volume: 1 µl 1. Caprosterol 2. Cholesterol 3. Cholestanol 4. Brassicasterol 5. Desmosterol 6. Ergosterol 7. Campasterol 8. Stigmasterol 9. Unknown 10. beta-sitosterol 11. Lanosterol FOO 18 Copyright SGE Analytical Science Pty Ltd July 2008
19 FOO 19 - FOOD PLANT STEROLS ISOTHERMALLY ON BPX5 Column Part No.: Phase: BPX5, 0.25 µm 30 m x 0.22 mm ID Initial Temp.: 310 C Detector: FID, 360 C Injector Mode: Split, 100:1 He, 20 psi Injection Volume: 1 µl 1. Coprosterol 2. Cholesterol 3. Cholestanol 4. Desmesterol 5. Brassicasterol 6. Ergosterol 7. Campasterol 8. Stigmasterol 9. Unknown 10. beta-sitosterol 11. Lanosterol FOO 19 Copyright SGE Analytical Science Pty Ltd July 2008
20 FOO 20 - FOOD PLANT STEROLS WITH TEMPERATURE PROGRAM ON BPX5 Column Part No.: Phase: BPX5, 0.25 µm 30 m x 0.22 mm ID Initial Temp.: 280 C, 1min Rate 1: 4 C/min Final Temp.: 350 C, 5min Detector: FID, 360 C Injector Mode: Split 100:1 He, 20 psi Injection Volume: 1 µl 1. Coprosterol 2. Cholesterol 3. Cholestanol 4. Desmosterol 5. Brassicasterol 6. Ergosterol 7. Campasterol 8. Stigmasterol 9. Unknown 10. beta-sitosterol 11. Lanosterol FOO 20 Copyright SGE Analytical Science Pty Ltd July 2008
21 FOO 21 - FOOD PLANT STEROLS ON A 0.53 MM ID BPX5 COLUMN Column Part No.: Phase: BPX5, 1.0 µm 30 m x 0.53 mm ID Initial Temp.: 320 C Detector: FID, 360 C Injector Mode: split 100:1 He, 3 psi Injection Volume: 1 µl 1. Coprosterol 2. Cholesterol 3. Cholestanol 4. Desmosterol 5. Brassicasterol 6. Ergosterol 7. Campasterol 8. Stigmasterol 9. Unknown 10. beta-sitosterol 11. Lanosterol FOO 21 Copyright SGE Analytical Science Pty Ltd July 2008
22 FOO 22 - FOOD COD OIL FATTY ACIDS (UNDERIVATISED) ON BP21 Column Part No.: Phase: BP21, 0.25 µm 25 m x 0.32 mm ID Initial Temp.: 70 C, 0.5 min Rate 1: 6 C/min Final Temp.: 260 C, 10 min Detector: FID, 280 C Injector Mode: Split, 50:1 He, 15 psi Injection Volume: 1 µl Injection Temp: 280 C 1. C14:0 2. C16:0 3. C16:1 4. C18:0 5. C18:1 6. C18:2 7. C18:3 8. C20:0 9. C22:0 10. C24:O Int Methylbutanoic Acid Int. 2. Octanoic Acid FOO 22 Copyright SGE Analytical Science Pty Ltd July 2008
23 FOO 23 - FOOD FREE FATTY ACID ANALYSIS ON BP21 Column Part No.: Phase: BP21, 0.5 µm Film 25 m x 0.53 mm ID Initial Temp.: 90 C, 1min Rate 1: 8 C/min Final Temp.: 160 C Detector: FID, 300 C Injector Mode: Split, 100:1 He, 5 ml/min Injection Volume: 1 µl 1. Acetic Acid 2. Propionic Acid 3. Isobutyric Acid 4. Butyric Acid 5. Isovaleric Acid 6. Valeric Acid 7. Unknown 8. Isocaproic Acid FOO 23 Copyright SGE Analytical Science Pty Ltd July 2008
24 FOO 24 - FOOD ANALYSIS OF WINE ON BP20 Column Part No.: Phase: BP20, 1.0 µm 25 m x 0.32 mm ID Initial Temp.: 40 C, 2 min Rate 1: 5 C/min Temp 2: 50 C Rate 2: 15 C/min Final Temp.: 190 C H 2, 6 psi Injection Mode: 2 µl 1. Acetaldehyde 2. Ethyl Acetate 3. Methanol 4. Isopropanol 5. Ethanol 6. Propanol 7. Isobutanol 8. Isoamyl Alcohol 9. Acetic Acid 10. Unknown FOO 24 Copyright SGE Analytical Science Pty Ltd July 2008
25 FOO 25 - FOOD TOCOPHEROL ISOMER MIXTURE ON BPX5 Column Part No.: Phase: BPX5, 0.25 µm 15 m x 0.32 mm ID Initial Temp.: 70 C, 1 min Rate 1: 25 C/min Temp 2: 280 C Rate 2: 5 C/min Final Temp.: 330 C, 5 min He, 7 psi Detector: F.I.D., 340 C Injector Mode: Cool on-column (OCI-5) Injection Volume: 0.5 µl FOO 25 Copyright SGE Analytical Science Pty Ltd July 2008
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