3) How many different amino acids are proteogenic in eukaryotic cells? A) 12 B) 20 C) 25 D) 30 E) None of the above
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1 Suggesting questions for Biochemistry 1 and 2 and clinical biochemistry 1) Henderson Hasselbalch Equation shows: A) The relationship between ph and the concentration of an acid and its conjugate base B) The relationship between ph and pk a C) Only the concentration of an acid in the solution D) Only the conjugate base in the solution E) A and B are correct 2) What is/are true about buffers: A) Buffer resists changes in solution ph B) Maximum buffering capacity is at or near the pk a of an acid C) One ph unit is equivalent to a 10 fold change in H+ ion concentration. D) Acetic Acid / Acetate is an example of buffer system in human cells E) A, B and C are correct 3) How many different amino acids are proteogenic in eukaryotic cells? A) 12 B) 20 C) 25 D) 30 E) None of the above 4) What is/are true about protein? A) Liner of amino acids B) A big molecules with multipurpose C) Contains different functional groups D) It is flexible 5) Identify which of the following terms refers to the overall three dimensional structure of a protein 1) Primary structure 2) Tertiary structure 3) Quaternary structure 4) Secondary structure 5) B and C are correct
2 6) Isolation of protein according to the charge of the protein, one should use: A) Affinity chromatography B) Gel filtration chromatography C) Ultracentrifugation D) Ion exchange chromatography E) SDS Polyacrylamide Electrophoresis 7) Protein secondary structure contains: A) Alpha helixes B) Beta sheets C) Turns D) Loops 8) Enzymes are: A) Proteins B) Carbohydrates C) Lipids D) Nucleic acids E) Ions 9) Enzymes: A) are specific B) catalyze reactions under mild conditions C) are regulated (controlled) D) some of them need cofactor and coenzymes 10) An enzyme functions by: A) lowering the activation energy of the reaction B) shifting the reaction balance to the right C) increase collides (hits) between the reactants D) giving more energy to the reaction E) stabilize the products
3 11) Coenzymes are A) vitamins B) ions C) organic compounds D) metals E) A and C 12) The number of molecules of substrates converted to product per enzyme molecule per second is called the? D A) Optimum number B) Maximum reaction rate / Vmax C) Michaelis Menten Constant/Km D) Turnover number / kcat E) Line weaver Plot 13) The most significance of Km is: B A) To know the enzyme regulation ways B) It indicates the relative suitability of alternate substrates for an enzyme C) To determine the ½ Vmax D) To calculate the substrate concentration for the reaction E) None of the above 14) What is / are true about inhibitor E A) It is any substances that reduce the reaction rate B) It is any substances that inactivates the enzyme of the reaction C) It is reversible D) Changes always both Vmax and Km E) A and C are correct 15) Enzymes can be regulated through E A) Covalent modifications B) Allosteric effects C) Enzyme availability D) Non covalent interactions
4 16) The lipids involve: A) Phospholipids B) Triglycerides C) Steroids/Sterols D) Fat soluble vitamins 17) An omega 3 fatty acid has the double bond A) at position 3 from the begin of fatty acid B) at position 3 from the end of fatty acid C) in the middle of fatty acids D) at the end E) none of the above 18) Cholestrol is precursor of: D A) Steroid hormones B) Vitamin A C) Eicosinodes D) A and E are correct E) Bile acids 19) Ionophores are A) Ion Carriers B) Ion channel C) Can be isolated from microorganisms D) Used as antibiotics 20) Cellular compartments permit for: A) Specialized environments B) Place to perform important process C) Chemical/electrical gradients D) Fusion with other organelles E) A, B and C are correct
5 21) The most common type of DNA is A) A type B) Z type C) B type D) G type E) A and Z 22) Posttranslational modification involve A) Signal sequence removing B) Glycosylation only in eukaryotes C) Folding of the protein D) Formation of S S bonds 23) Which antibiotic does inhibit protein synthesis only in eukaryotes? A) Tetracyclin B) Neomycin C) Erythromycin D) Cycloheximide E) Chloramphenicol 24) What is true about genetic code A) Genetic code is overlapping B) Genetic code is the relation between the sequence of bases in DNA (or mrna) and the sequence of amino acids in proteins C) Has punctuations D) Genetic code is not degenerate E) universal 25) The function of trnas in the cell is A) Carrying the protein B) Carrying the amino acids C) Recognition of the codons D) Interaction with DNA E) B and C are correct
6 26) A biological redox reaction always involves: A) A loss of electrons. B) A gain of electrons C) A reducing/oxidizing agent D) Biological materials 27) Adenylate cyclase is an enzyme that catalysis A) Formation of cadp B) Formation of CGMP C) Formation camp D) Ip3 E) A and B 28) G protein losses its activity when A) beta subunit dissociated from the effector protein B) Alpha, beta and gamma subunits bind GTP C) Protein kinase become inactive D) GTP is hydrolyzed to GDP E) A, B and C 29) Cholera and pertussis toxins bind to A) The ligands B) A proteins C) Cytochrome P450 D) G proteins E) Hydrogenase 30) The cytochrome c oxidase complex: A) Accepts electrons from cyt c. B) Donates four electrons to O2. C) Produces 2 H2O per O2 reduced. D) All of the above are correct. E) None of the above
7 Solutions: 1 E 2 E 3 B 4 E 5 B 6 D 7 E 8 A 9 E 10 A 11 E 12 D 13 B 14 E 15 E 16 E 17 B 18 D 19 E 20 E 21 C 22 E 23 D 24 B 25 E 26 E 27 D 28 D 29 D 30 D
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