ab Oil Red O (Lipid Stain)

Transcription

1 Version 2 Last updated 19 December 2018 ab Oil Red O (Lipid Stain) For the histological visualization of fat cells and neutral fat. View kit datasheet: (use for China, or for Japan) This product is for research use only and is not intended for diagnostic use. Copyright 2018 Abcam. All rights reserved

2 Table of Contents 1. Overview 1 2. Materials Supplied and Storage 2 3. Materials Required, Not Supplied 3 4. General guidelines, precautions, and troubleshooting 4 5. Staining Protocol 5 6. FAQs / Troubleshooting 6 7. Typical Data 7 8. Notes 8 Copyright 2018 Abcam. All rights reserved

3 1. Overview The Oil Red O (Lipid Stain) (ab150678) is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used only on frozen tissue sections, fresh smears, or touch preps. Staining Interpretation: Fat Cells Red Neutral Fat Red Nuclei Blue Control Tissue: Any frozen section containing fat. ab Oil Red O (Lipid Stain) 1

4 2. Materials Supplied and Storage Store kit at room temperature immediately on receipt and check below for storage for individual components. Kit can be stored for 1 year from receipt, if components have not been reconstituted. Keep away from open flame and refer to the safety datasheet. Item Quantity Storage temperatur e (before prep) Hematoxylin (Modified Mayer s) 125 ml RT Propylene Glycol 500 ml RT Oil Red O Solution 125 ml RT ab Oil Red O (Lipid Stain) 2

5 3. Materials Required, Not Supplied These materials are not included in the kit, but will be required to successfully perform this assay: Aqueous Mounting Medium. ab Oil Red O (Lipid Stain) 3

6 4. General guidelines, precautions, and troubleshooting Please observe safe laboratory practice and consult the safety datasheet. For general guidelines, precautions, limitations on the use of our assay kits and general assay troubleshooting tips, particularly for first time users, please consult our guide: For typical data produced using the assay, please see the assay kit datasheet on our website. ab Oil Red O (Lipid Stain) 4

7 5. Staining Protocol Equilibrate all materials and prepared reagents to room temperature just prior to use and gently agitate. 5.1 Prepare fresh or frozen tissue sections as usual. 5.2 Place slide in Propylene Glycol for 2 minutes. 5.3 Incubate slide in Oil Red O Solution for 6 minutes. 5.4 Prepare a mixture of 85% Propylene Glycol in distilled water and differentiate the tissue section in this mixture for 1 minute. 5.5 Rinse slide in 2 changes of distilled water. 5.6 Incubate slide in Hematoxylin for 1-2 minutes. 5.7 Rinse slide thoroughly in tap water. 5.8 Rinse slide in 2 changes of distilled water. 5.9 Coverslip using an aqueous mounting medium. ab Oil Red O (Lipid Stain) 5

8 6. FAQs / Troubleshooting General troubleshooting points are found at ab Oil Red O (Lipid Stain) 6

9 7. Notes ab Oil Red O (Lipid Stain) 7

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12 Technical Support Copyright Abcam. All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. Austria wissenschaftlicherdienst@abcam.com France supportscientifique@abcam.com Germany wissenschaftlicherdienst@abcam.com Spain soportecientifico@abcam.com Switzerland technical@abcam.com Deutsch: Français: UK, EU and ROW technical@abcam.com +44(0) Canada ca.technical@abcam.com US and Latin America us.technical@abcam.com Asia Pacific hk.technical@abcam.com (852) China cn.technical@abcam.com / Japan technical@abcam.co.jp +81-(0) Singapore sg.technical@abcam.com Australia au.technical@abcam.com +61-(0) New Zealand nz.technical@abc.com +64-(0) Copyright 2018 Abcam. All rights reserved