BIOLOGICAL PRETREATMENT AND ETHANOL PRODUCTION FROM OLIVE CAKE
|
|
- Samson Paul
- 6 years ago
- Views:
Transcription
1 BIOLOGICAL PRETREATMENT AND ETHANOL PRODUCTION FROM OLIVE CAKE E. JURADO, H.N. GAVALA, G.N. BAROI and I.V. SKIADAS,* Copenhagen Institute of Technology (Aalborg University Copenhagen), Section for Sustainable Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering, Lautrupvang 5, DK 275 Ballerup, Denmark. *Corresponding author: telephone , fax Keywords: enzymatic hydrolysis, ethanol, olive cake, white rot fungi, Phaneroachaete chrysosporium, Ceriporiopsis subvermispora, Ceriolopsis polyzona, Thermoanaerobacter ethanolicus. Abstract Olive oil is one of the major Mediterranean products, whose nutritional and economic importance is well-known. However the extraction of olive oil yields a highly contaminating residue that causes serious environmental concerns in the olive oil producing countries. The olive cake (OC) coming out of the three-phase olive oil production process could be used as low price feedstock for lignocellulosic ethanol production due to its high concentration in carbohydrates. However, the binding of the carbohydrates with lignin may significantly hinder the necessary enzymatic hydrolysis of the polymeric sugars before ethanol fermentation. Treatment with three white rot fungi, Phaneroachaete chrysosporium, Ceriporiopsis subvermispora and Ceriolopsis polyzona has been applied on olive cake in order to investigate the potential for performing delignification and thus enhancing the efficiency of the subsequent enzymatic hydrolysis and ethanol fermentation process steps. It has been concluded that the conditions tested were not adequate for reaching satisfactory delignification and thus studying different conditions (humidity, ph and nitrogen levels) is necessary. Another possibility for lowering the cost of bioethanol is the use of microbial strains, which possess the ability for hydrolysis of complex carbohydrates. Thus, the addition of enzymes could be eliminated to a minimum extent for reaching a satisfactory degree of fermentable sugars release from a biomass. In that concept, the hydrolytic, ethanol-producing Thermoanaerobacter ethanolicus has been applied on olive cake supplemented with glucose in order to evaluate any inhibitory effect that olive cake might have on the microbial growth and metabolism. It was shown that the strain was able to grow and produce ethanol up to a TS content of g per L. Further investigation of the ph role on the ethanol yield will take place.
2 - INTRODUCTION Olive oil is one of the major Mediterranean products, whose nutritional and economic importance is well-known. However the extraction of olive oil yields a highly contaminating residue that causes serious environmental concerns in the olive oil producing countries and unfortunately, this is a problem which has not been solved yet []. Two different methods are mainly used for the extraction of olive oil: the pressing and the centrifugation of the precrashed olives. The centrifugation and especially the three-phase process is the most common in the olive oil industry. In the three-phase process, centrifugation is directly applied to the crashed olives mass, resulting in three phases: olive oil, wastewater (mixture of vegetation and process water) and olive cake (solid residue from the olives with about 5% water content). The olive cake (OC) could be used as low price feedstock for lignocellulosic ethanol production due to its high concentration in carbohydrates. However, the binding of the carbohydrates with lignin may significantly hinder the necessary enzymatic hydrolysis of the polymeric sugars before ethanol fermentation. Enzymes comprise an estimated 2 4% of cellulosic ethanol production costs [2] and thus they are one of the main factors affecting the financial viability of bioethanol. To decrease the use of enzymes and increase their efficiency, several physico-chemical processes have been tested as a previous step to enzymatic hydrolysis, but these processes present their own limitations as high costs, or production of inhibitory compounds. On the other hand, white rot fungi (WRF) are the most efficient lignin degraders in nature [3], which posses the unique ability to efficiently degrade lignin. Some WRF are the only organisms, which are able to perform selective delignification without consuming the valuable carbohydrates under specific conditions. Two main characteristics of the OC may make it suitable for fungal pretreatment. The first characteristic is the activation of the WRF s enzymatic system by the presence of polyphenolic compounds in olive-mill effluents [4] in combination with the existing pectinase activity [5] and its high dry matter content (up to 5%). Several studies have shown a better performance of the WRF in presence of elevated oxygen levels [6, 7]. Also, the substrate must possess enough moisture to support growth and metabolism of microorganism [8]. Another possibility for lowering the cost of bioethanol is the use of microbial strains, which possess the ability for hydrolysis of complex carbohydrates. Thus, the addition of enzymes could be eliminated to a minimum extent for reaching a satisfactory degree of fermentable sugars release from a biomass. The aim of the present work was to study the capability of selected fungi either to perform delignification or to alter the lignin structure of the olive cake and thus enhancing the efficiency of the subsequent enzymatic hydrolysis and ethanol fermentation process steps. Three WRF strains were selected based on previous studies [9,,, 5] and screened for their ability to grow on OC and to facilitate sugars release during enzymatic hydrolysis: Phaneroachaete chrysosporium, Ceriporiopsis subvermispora and Ceriolopsis polyzona. Also, the hydrolytic, ethanol-producing Thermoanaerobacter ethanolicus has been applied on olive cake supplemented with glucose in order to evaluate any inhibitory effect that olive cake might have on the microbial growth and metabolism. The research performed so far is part of a more general research frame, which is shown in figure. 2- MATERIALS AND METHODS 2.-Analytical Methods Dry matter content was determined as total solids (TS) concentration and was carried out according to Standard Methods [2]. Detection and quantification of sugar monomers,
3 glucose and xylose as well as end-fermentation products, i.e. ethanol, acetic and lactic acids was made by HPLC-RI equipped with an Aminex HPX-87H column (BioRad) at 6 C. A solution of 4mmol L - H 2 SO 4 was used as eluent at a flow rate of.6ml min -. Prior to HPLC analysis, ml of sample was acidified with μl of 2% H 2 SO 4. Centrifugation at, rpm for min followed by filtration through a.45μm membrane filter was then applied. Three groups of carbohydrates were analyzed in the olive pulp: the st group was the total carbohydrates, including those bound in the lignocellulosic biomass, the 2nd group was the soluble carbohydrates and the last one was the simple sugars. An overview and details of the methodology used for carbohydrates and lignin determination can be found in [3]. olive cake pretreatment, biological (fungal) or physicochemical (alkali treatment) A. SHF with Celluclast.5L & Novozyme-88 and T. ethanolicus enzymatic hydrol. ethanol prod. B. SSF with Celluclast.5L & Novozyme-88 and T. ethanolicus enzymatic hydrolysis and ethanol production (SSF) C. DMC with Celluclast.5L & Novozyme-88 and T. ethanolicus direct microbial conversion (DMC) (no enzymes addition) Figure : Research outline for bioethanol production from olive cake (SHF: separate hydrolysis and fermentation, SSF: simultaneous saccharification and fermentation). 2.2-Enzymatic hydrolysis The possibility of applying enzymatic hydrolysis for release of the sugars contained in the olive cake was assessed using two different combinations of enzymes. The assessment was done as batch tests with 5 g TS olive cake and different enzyme loadings. The first enzymes combination was a mixture of Celluclast.5L (an enzyme mixture containing cellulase and hemicellulase activities, CAS number: ) and Novozyme 88 (an enzyme containing beta-glucosidase activity, MDL number: MFCD3628) and the second, which was named Novozymes Biomass Kit, consisted of five different enzymes (NS53, NS5, NS52, NS53, NS222) containing cellulase, beta-glucosidase, arabinase, hemicelluloses, pectinase and xylanase activities. All enzymes were kindly provided by Novozymes A/S (Bagsværd, Denmark). Enzyme activity was measured by Filter Paper assay [4, 5]. Novozyme 88 was supplemented with Celluclast at a ratio of :3 (volume based) while the NS53:NS5:NS52:NS53:NS222 ratio was 5:.5::.25:5. The olive cake was distributed to infusion flasks before being heated to 2 C C for 2 min. After cooling, enzyme loadings of.,.34 and.58 g of each of the two enzymes combinations per g TS were added to the batches. The enzymatic hydrolysis was tested at 5 C with agitation at 2 rpm for a period of four. Glucose and xylose concentrations were determined after the completion of the experiment. All batch tests were run in triplicates. For enzymatic hydrolysis of the fungal pretreated olive cake (described in the following) a mixture of the Novozymes Biomass kit was used at a loading of 5 FPU per g TS olive cake. The enzymatic hydrolysis was performed at the conditions described above. 2.3-Biological (fungal) pre-treatment P. chrysosporium strain DSM 699, C. subvermispora strain CBS and C. polyzona strain MUCL were obtained from the Deutsche Sammlung von Microorganismen und Zellkulturen (DSMZ), CBS-KNAW fungal biodiversity centre and Belgian Co-ordinated
4 Collections of Micro-organisms (BCCM), respectively. The medium used for growing the cultures was the DSM 9 for P. chrysosporium and MA2 (malt agar 2%) for C. subvermispora and C. polyzona. Agar-grown cultures were maintained at -8 C in % glycerol in water. P. chrysosporium, C. subvermispora and C. polyzona cultures were first regrown in agar plates (5 g of agar per L medium for P. chrysosporium and 2 g per L for C. subvermispora and C. polyzona). Subsequently they were transferred in autoclaved (2 C) infusion bottles containing 3 ml of their respective growth medium and placed in a rotary shaker at 5 rpm at 37, 32 and 2 C respectively. The rubber stopper of the infusion bottles was equipped with a glass tube filled with cotton in order to ensure sterile oxygen transfer. The cultures were harvested, rinsed with water and homogenized prior to olive cake inoculation (% v/w). Delignification experiments with P. chrysosporium and C. polyzona: A thin layer of g olive cake was added in 2 infusion bottles and autoclaved at 2 C for 2 min. Subsequently, the bottles were inoculated with the fungi and incubated at 37 C in a rotary shaker at 5 rpm. Triplicates were analyzed for lignin and carbohydrates content at, 4,, 5, 9 and 25 after inoculation. One triplicate control with olive cake without inoculation was running in parallel. Sugar release experiments with P. chrysosporium, C. subvermispora and C. polyzona pretreatment followed with enzymatic treatment: Sugars release after 7 and 4 fungal pretreatment of the olive cake followed by 4 enzymatic hydrolysis was investigated as shown in figure 2. Also, experiments with P. Chrysosporium grown in medium without peptone (elimination of the nitrogen content) and forced aeration using an air compressor have been carried out as well. After 4 of enzymatic treatment all triplicates were analyzed for free and soluble sugars content. Autoclaved olive cake control vials, no fungi addition, x6 vials inoculated with fungi, x2 after 7 and 4 after 7 and 4 enzyme addition, 5 FPU per g TS autoclavation at 2 C for 2 min free and soluble sugars analysis Figure 2. Experimental set-up for fungal pre-treatment followed by enzymatic hydrolysis. 2.4-Microbial batch fermentation tests T. ethanolicus, strain DSM 2246 was obtained from the Deutsche Sammlung von Microorganismen und Zellkulturen (DSMZ) culture collection and was maintained in DSM 6 medium. Stock cultures were stored at -8 C in % glycerol and inoculation cultures were transferred once before use. All cultures were grown in a N 2 atmosphere, at 7 C. Experiments with T. ethanolicus on glucose medium supplemented with different olive cake contents were carried in triplicates in serum vials sealed with rubber stoppers. Glucose and products concentrations as well as ph were followed throughout the experiment. 3- RESULTS AND DISCUSSION 3.-Olive cake characterization and enzymatic hydrolysis Olive cake was characterized by a 5,7 ±,4 % TS content and a ph value of 5,5. The total carbohydrates in olive pulp were composed by glucose and xylose (3,3 ±,2 and 6,4 ±,3 g per g TS, respectively) with the soluble and simple sugars comprising a small fraction
5 of them (4 ±,6 and,34 ±,5 g-soluble and free glucose per g TS and,9 ±,22 and,94 ±,4 g-soluble and free xylose per g TS, respectively). The low content of free and soluble sugar compared to the total carbohydrates implies that efficient hydrolysis should considerably increase the availability of sugars for ethanol fermentation. Lignin content is also high (45,8 g per g TS) and therefore reduction of lignin will further facilitate the efficiency of hydrolysis and ethanol fermentation steps. The enzymatic hydrolysis or raw olive cake with both enzyme mixtures (Celluclast.5L- Novozyme-88 and Novozymes Biomass Kit) significantly increased the soluble sugars concentration (Figure 3). Based on the results obtained, the use of the Novozymes Biomass Kit was more effective for olive cake hydrolysis and therefore the Novozymes Biomass kit was used for all subsequent experimental tests at a loading of 5 FPU per g TS. 3, 2,,,5, glucose xylose control () 8, 29,7 5 FPU per g TS (a) 3, 2,,,5, glucose xylose control () 6,2 2,3 35,8 FPU per g TS Figure 3: Soluble sugars released from olive cake after enzymatic hydrolysis with (a) Celluclast.5L mixed with Novozyme-88 and (b) Novozymes Biomass Kit 3.2-Biological (fungal) pre-treatment The results from the delignification experiments with P. chrysosporium and C. polyzona are shown in Figure 4. It seems that after of fungal growth some delignification started to occur in the experiments with P. chrysosporium reaching approximately 5%, while at the same time a decrease of total glucose content was observed while xylose content remained unaltered. On the other hand, the experimental data with C. polyzona showed that no lignin degradation or total carbohydrates decrease took place. It is noticeable that the standard deviation of lignin and total carbohydrates was considerably high, thus not allowing for solid conclusions to be drawn. (b) lignin - P. chrysosporium lignin-c. polyzona 25 2 glu - P. chrysosporium glu-c. polyzona xyl-p. chrysosporium xyl-c. polyzona Figure 4. Lignin, total-glucose and total xylose content in g per g TS versus time in during delignification experiments with P. chrysosporium and C. polyzona. In figures 5a, 5b and 5c, soluble sugars release after enzymatic treatment of fungal pre-treated olive cake by the three strains is shown. In all three cases studied, the sugars release was lower or comparable to the control (no fungal pre-treatment). Data from experiments with P. Chrysosporium grown in medium without peptone (elimination of the nitrogen content) were comparable with the ones shown in figure 5a. Also, forced aeration did not influence the
6 sugars release. Investigation of delignification potential at different conditions, i.e. different humidity, ph and nitrogen levels, is necessary. 3 control without sterilisation with sterilization (a) 3 control without sterilisation with sterilization (b) 2 2,5,5 glucose-7 glucose-4 xylose-7 xylose-4 glucose-7 glucose-4 xylose-7 xylose control without sterilisation with sterilization (c) 3 2 glucose-7 glucose-4 xylose-7 xylose-4 Figure 5. Soluble sugars release in g per g TS after enzymatic treatment of fungal pretreated olive cake by (a) P. chrysosporium, (b) C. subvermispora and (c) C. polyzona. 3.3-Microbial batch fermentation tests Glucose and products concentration as well as ph values in the experiments with T. ethanolicus supplemented with glucose and different amounts of olive cake are shown in Table. Complete consumption of glucose was observed in the experiments with up to g TS olive cake added per L of medium within three, while the vials with 5 and 25 g TS per L showed limited and no consumption, respectively. It is noticeable that the higher the amounts of olive cake the lower the ethanol yield was (.9,. and.63 g per g in the control vials and vials supplements with 5 and g TS per L olive cake, respectively). This can be attributed either to the lower ph values and/or to potential presence of inhibitors in the olive cake. It is more likely that the ethanol yield was dependent on the ph values while the absence of microbial activity at 25 g TS olive cake per L was due to inhibitory effects since a ph of 5,5 allowed still for microbial growth. Future studies will include experiments with high TS concentration and controlled ph in order to clarify this issue. Glucose at time Substrate in g/l Products concentration in g/l and ph values after 3 Glucose after 3 Lactic acid Acetic acid Ethanol ph Control 5,47 ±,39,74 ±,4,22 ±,2,5 ±,6 6,5 5 g TS/L 6,4 ±,68 2,84 ±,27,25 ±,2,65 ±,5 5,7 g TS/L 7,33 ±,3 4 ±,6,23 ±,46 ±,6 4,8 5 g TS/L 8,97 ±,74 3,8 ±,54 2,9 ±,3,33 ±,2 4,8 25 g TS/L,7 ± 9 9 ±,49,2 ±, 5,5 Table. Glucose and products concentration as well as ph values in the experiments with T. ethanolicus supplemented with glucose and different amounts of olive cake.
7 4- CONCLUSIONS Treatment with three white rot fungi, Phaneroachaete chrysosporium, Ceriporiopsis subvermispora and Ceriolopsis polyzona has been applied on olive cake in order to investigate the potential for performing delignification and thus enhancing the efficiency of the subsequent enzymatic hydrolysis and ethanol fermentation process steps. It has been concluded that the conditions tested were not adequate for reaching satisfactory delignification and thus studying different conditions (at different humidity, ph and nitrogen levels) is necessary. Also, the hydrolytic, ethanol-producing Thermoanaerobacter ethanolicus has been applied on olive cake supplemented with glucose in order to evaluate any inhibitory effect that olive cake might have on the microbial growth and metabolism. It was shown that the strain was able to grow and produce ethanol up to a TS content of g per L. Further investigation of the ph role on the ethanol yield will take place. ACKNOWLEDGEMENT: The authors wish to thank the Commission of the European Communities for the financial support of this work under FP7 grant No (Acronym: ETOILE). REFERENCES [] Ballesteros, I., Olivia, J.M., Saez, F., Ballesteros, M.: Ethanol production from lignocellulosic byproducts of olive oil extraction. Applied Biochemistry and Biotechnology 9-93, (2) [2] Sainz, M.B.: Commercial cellulosic ethanol: The role of plant-expressed enzymes. In vitro cellular & developmental biology-plant 45, (29) [3] Hatakka, A.: Lignin-moifiing enzymes from selected white-rot fungi. production and role in lignin degradation. FEMS Microbiolgy Reviews 3, (994) [4] Saavedra, M., Benitez, E., Cifuentes, C., Nogales, R.: Enzyme activities and chemical changes in wet olive cake after treatment with pleurotus ostreatus or elisea fetida. Biodegradation 7, 93-2, 26, [5] Chi,Y., Hatakka, A., Maijala, P.: Can co-culturing of two white-rot fungi increase lignin degradation and the production of lignin-degrading enzymes?. International Biodeteroriation and Biodegradation 59, 32-39, (27) [6] Sanchez, C.: Lignocellulosic residues: biodegradation and bioconversion by fungi. Biotechnology Advances 27, 85-94, (29) [7] Kent kirk, T., Farrel, R.L.: Enzymatic commbustion : The micorbial degradation of lignin. Ann. Rev. Microbiol. 4, (987) [8] Pandey, A.: Solid-state fermentation. Biochemical Engineering Journal 3, 8-84 (23) [9] Jaouani, A., Sayadi, S., Vanthournhout, M., Penninckx, M.J.: Potent fungi for decolourisation of olive oli mill wastewater. Enzyme and Microbial Technology 33, (23). [] Aloui, F., Abid, N., Roussos, S., Sayadi, S.: Decolorization of semisolid olive residues of alperujo during the solid state fermentation by Phaneroachaete crysosporum, Trametes versicolor, Pycnoporus cinnabarinus and Aspergillus niger. Biochemical Engineering Journal 35, 2-25, (27) [] Dorado, J., Almendros, G., Camarero, S.; Martinez, A.T.; Vares, T.; Hatakka, A.: Transformation of wheat straw in the course of solid-state fermentation by four ligninolytic basidiomycetes. Enzyme and Microbial Technology 25, 65-62, (999) [2] APHA, AWWA, WPCF. In: Franson, M.A. (ed.) Standard methods for the examination of water and wastewater. Washington, DC: American Public Health Association (995). [3] Haagensen, F., Skiadas, I.V., Gavala, H.N., Ahring, B.K.: Pre-treatment and ethanol fermentation potential of olive pulp at different dry matter concentrations. Biomass and Bioenergy 33, (29). [4] Mandels, M., Andreotti, R., Roche, C.: Measurement of saccharifying cellulase. Biotechnol. Bioeng. Symp. 6, 2-33 (976). [5] Ghose, TK.: Measurement of cellulose activities. Pure & Appl. Chem. 59 (2), (987).
In this study, effect of different high-boiling-organic solvent (ethanolamine, diethylene glycol and
ISESCO JOURNAL of Science and Technology Vol. 12 No 21 High Boiling Solvent Pre-treatment of Hazelnut Shells for Enzymatic Hydrolysis Emir Zafer Hoşgün, Berrin Bozan Anadolu University, Engineering Faculty,
More informationEFFECT OF HEMICELLULOSE LIQUID PHASE ON THE ENZYMATIC HYDROLYSIS OF AUTOHYDROLYZED EUCALYPTUS GLOBULUS WOOD
S05-036 EFFECT OF HEMICELLULOSE LIQUID PHASE ON THE ENZYMATIC HYDROLYSIS OF AUTOHYDROLYZED EUCALYPTUS GLOBULUS WOOD Romaní, Aloia; Ruiz, Héctor A. *; Pereira, Francisco B; Domingues, Lucília; Teixeira,
More informationImprovement of enzymatic hydrolysis of a marine macro-alga by dilute acid hydrolysis pretreatment
Improvement of enzymatic hydrolysis of a marine macro-alga by dilute acid hydrolysis pretreatment Parviz Yazdani 1*, Keikhosro Karimi 1,2, Mohammad J. Taherzadeh 2 1 Department of Chemical Engineering,
More informationEthanol production from alfalfa fiber fractions by saccharification and fermentation*
PROCESS BIOCHEMISTRY ELSEVIER Process Biochemistry 36 (2001) 1199-1204 www.elsevier.com/locate/procbio Ethanol production from alfalfa fiber fractions by saccharification and fermentation* Hassan K. Sreenath
More informationDecolorization of olive mill wastewaters by co-culture of Geotrichum candidum and Lactobacillus plantarum
Proceedings of International Symposium on Environmental Pollution Control and Waste Management 7-0 January 00, Tunis (EPCOWM 00), p.6-66. Decolorization of olive mill wastewaters by co-culture of Geotrichum
More informationCHAPTER NO. TITLE PAGES
BRIEF CONTENTS CHAPTER NO. TITLE PAGES PREFACE 1 1 INTRODUCTION 3 2 REVIEW OF LITERATURE 6 3 MATERIALS & METHODS 70 4 OBSERVATIONS & RESULTS 110 5 DISCUSSION 222 6 SUMMARY & CONCLUSIONS 243 BIBLIOGRAPHY
More informationHydrothermal pretreatment of biomass for ethanol fermentation
Hydrothermal pretreatment of biomass for ethanol fermentation Yukihiko Matsumura Hiroshima University 1 Dec. 10-12, 2012 JAPANESE-DANISH JOINT WORKSHOP Future Green Technology Hakata, Japan 緒言 First and
More informationEFFECT OF LACCASE DOSAGE ON ENZYMATIC HYDROLYSIS OF STEAM- EXPLODED WHEAT STRAW
CELLULOSE CHEMISTRY AND TECHNOLOGY EFFECT OF LACCASE DOSAGE ON ENZYMATIC HYDROLYSIS OF STEAM- EXPLODED WHEAT STRAW ALFREDO OLIVA-TARAVILLA, * ELIA TOMÁS-PEJÓ, * MARIE DEMUEZ, * CRISTINA GONZÁLEZ-FERNÁNDEZ
More informationEffect of process conditions on high solid enzymatic hydrolysis of pre-treated pine
Effect of process conditions on high solid enzymatic hydrolysis of pre-treated pine Abstract Anders Josefsson Department of Chemical Engineering, Lund University, Sweden 213-6-12 In this study a relatively
More informationEthanol Production from the Mixture of Hemicellulose Prehydrolysate
Ethanol Production from the Mixture of Hemicellulose Prehydrolysate and Paper Sludge Li Kang, David Webster, Harry Cullinan and Y. Y. Lee Department of Chemical Engineering Auburn University 1 Outline
More informationEnzymatic Bioconversion and Fermentation of Corn Stover at High-solids Content for Efficient Ethanol Production
National Technical University of Athens School of Chemical Engineering Biotechnology Laboratory Industrial Waste & Wastewater Treatment & Valorization Enzymatic Bioconversion and Fermentation of Corn Stover
More informationChemical and Microbial Hydrolysis of Sweet Sorghum Bagasse for Ethanol Production
Chemical and Microbial Hydrolysis of Sweet Sorghum Bagasse for Ethanol Production Anusith Thanapimmetha 1,2, Korsuk Vuttibunchon 1, Maythee Saisriyoot 1,2, Penjit Srinophakun 1,2 * 1 Bioprocess Laboratory,
More informationTHE EFFECT OF DELIGNIFICATION PROCESS WITH ALKALINE PEROXIDE ON LACTIC ACID PRODUCTION FROM FURFURAL RESIDUES
THE EFFECT OF DELIGNIFICATION PROCESS WITH ALKALINE PEROXIDE ON LACTIC ACID PRODUCTION FROM FURFURAL RESIDUES Yong Tang, Lingxi Bu, Lihong Deng, Liwei Zhu, and Jianxin Jiang* Furfural residues produced
More informationScreening of Rice Straw Degrading Microorganisms and Their Cellulase Activities
Research 83 KKU Sci. J.37 (Supplement) 83-88 (2009) Screening of Rice Straw Degrading Microorganisms and Their Cellulase Activities Abstract Atcha Boonmee 1,2* Rice straw is one of the most abundant agricultural
More informationOPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE
Int. J. Chem. Sci.: 8(2), 2010, 909-913 OPTIMISATION OF XYLOSE PRODUCTION USING XYLANASE T. SATHISH a and N. Y. S. MURTHY * Department of Biotechnology, Malla Reddy Engineering College, HYDERABAD (A.P.)
More informationDeutscher Tropentag - Bonn, 9-11 October 2001
Deutscher Tropentag - Bonn, 9-11 October 21 Conference on International Agricultural Research for Development Tropical wood-decaying fungi as a means of conversion of agricultural plant residues: Influence
More informationMinimizing Wash Water Usage After Acid Hydrolysis Pretreatment of Biomass
University of Arkansas, Fayetteville ScholarWorks@UARK Biological and Agricultural Engineering Undergraduate Honors Theses Biological and Agricultural Engineering 5-2013 Minimizing Wash Water Usage After
More informationLiquid Hot Water Pretreatment of Corn Stover: Impact of BMR. Nathan S. Mosier and Wilfred Vermerris
Liquid Hot Water Pretreatment of Corn Stover: Impact of BMR Nathan S. Mosier and Wilfred Vermerris Acknowledgements Research, Inc. (CPBR), U.S. Department of Energy (DOE) Prime Agreement no. DEFG36-02GO12026.
More informationKinetics of Bioethanol Production from Glycerol by Enterobacter aerogenes
Available online at www.sciencedirect.com ScienceDirect Energy Procedia 61 (2014 ) 2244 2248 Abstract The 6 th International Conference on Applied Energy ICAE2014 Kinetics of Bioethanol Production from
More informationSaccharification of corncob using cellulolytic bacteria - Titi Candra Sunarti et al.
Saccharification of corncob using cellulolytic bacteria - Titi Candra Sunarti et al. Figure 2. (a) (b) (c) (d) Microscopic structures of (a) corncob, (b) delignified corncob, (c) cellulose fraction, (d)
More informationEvaluation of the Main Inhibitors from Lignocellulose Pretreatment for Enzymatic Hydrolysis and Yeast Fermentation
Evaluation of the Main Inhibitors from Lignocellulose Pretreatment for Enzymatic Hydrolysis and Yeast Fermentation Young Hoon Jung a and Kyoung Heon Kim b, * To produce cellulosic ethanol more economically,
More informationProduction of a cellulosic substrate susceptible to enzymatic hydrolysis from prehydrolyzed barley husks
Vol. 11 (2002): 51 58. Production of a cellulosic substrate susceptible to enzymatic hydrolysis from prehydrolyzed barley husks Ana Belén Moldes, José Manuel Cruz, José Manuel Domínguez and Juan Carlos
More informationEXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN
BIOLOGIA 2001, 47 (1&2), PP 47 52 ISSN 0006 3096 EXTRACTION OF THERMO-STABLE ALPHA AMYLASE FROM FERMENTED WHEAT BRAN *HAMAD ASHRAF, IKRAM UL HAQ, AND JAVED IQBAL Biotechnology Research Laboratory, Department
More informationIMPROVED PRETREATMENT PROCESS OF WHEAT STRAW WITH DIRECT STEAM INJECTION
IMPROVED PRETREATMENT PROCESS OF WHEAT STRAW WITH DIRECT STEAM INJECTION Patrick Ballmann *1, Michael Müller *1, Esther Gasser *2, Stefan Dröge *1, Helmut König *2 *1 Test and Research Institute Pirmasens
More informationBioconversion of agro-industrial wastes: optimization of the saccharification stage
Bioconversion of agro-industrial wastes: optimization of the saccharification stage M. González, C. Marzo, A.B. Díaz, A. Blandino*, I. Caro, Department of Chemical Engineering and Food Technology, Faculty
More informationLAP-019CS. Procedure Title: Author(s): Bonnie Hames, Fannie Posey-Eddy, Chris Roth, Ray Ruiz, Amie Sluiter, David Templeton.
Biofuels Program Biomass Analysis Technology Team Laboratory Analytical Procedure LAP-019CS Procedure Title: Hydrolysis of Corn Stover for Compositional Analysis Author(s): Bonnie Hames, Fannie Posey-Eddy,
More informationMODIFICATION OF WHEAT STRAW LIGNIN BY SOLID STATE FERMENTATION WITH WHITE-ROT FUNGI
MODIFICATION OF WHEAT STRAW LIGNIN BY SOLID STATE FERMENTATION WITH WHITE-ROT FUNGI Maria J. Dinis a, Rui M. F. Bezerra b, Fernando Nunes c, Albino A. Dias b, Cristina V. Guedes a, Luís M. M. Ferreira
More informationEffect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste
Biotechnological Communication Biosci. Biotech. Res. Comm. 8(1): 78-83 (2015) Effect of ph on the production of protease by Fusarium oxysporum using agroindustrial waste Rupali R. Deshmukh and N. N. Vidhale*
More informationStudy on moisture content of substrate (Sesamum oil cake and Rice chaff) on the yield of Citric acid
Int.J.Curr.Microbiol.App.Sci (14) 3(5): 138-144 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-776 Volume 3 Number 5 (14) pp. 138-144 http://www.ijcmas.com Original Research
More informationCellulase Inhibitors/Deactivators in Lignocellulosic Biomass
Cellulase Inhibitors/Deactivators in Lignocellulosic Biomass Youngmi Kim *, Eduardo Ximenes, Nathan S. Mosier and Michael R. Ladisch LORRE, Purdue Univ. 32 nd Symposium on Biotechnology for Fuels and Chemicals
More informationCELLULASE from PENICILLIUM FUNICULOSUM
CELLULASE from PENICILLIUM FUNICULOSUM Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in FNP
More informationFungal Enzymes in Decolorizing Paper Pulp
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 11 (2017) pp. 2873-2879 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.611.339
More informationCaroline Vanderghem, Nicolas Jacquet, Christophe Blecker, Michel Paquot
Pretreatments and enzymatic hydrolysis of Miscanthus x giganteus for oligosaccharides production: delignification degree and characterisation of the hydrolysis products Caroline Vanderghem, Nicolas Jacquet,
More informationIJREAT International Journal of Research in Engineering & Advanced Technology, Volume 1, Issue 2, April-May, 2013 ISSN:
A Comparative Overview of Ethanol Production from Cereal Grains and Potato by Enzymatic Treatment Soumitra Banerjee 1, Debalina Kundu 2 Dept of Food Technology, Techno India Saltlake, Kolkata 700091 Abstract
More informationOPTIMIZATION OF RICE BRAN HYDROLYSIS AND KINETIC MODELLING OF XANTHAN GUM PRODUCTION USING AN ISOLATED STRAIN
International Journal of Science, Environment and Technology, Vol. 4, No 2, 2015, 285 292 ISSN 2278-3687 (O) 2277-663X (P) OPTIMIZATION OF RICE BRAN HYDROLYSIS AND KINETIC MODELLING OF XANTHAN GUM PRODUCTION
More informationTHE RELATIONSHIP BETWEEN TWO METHODS FOR EVALUATING FIVE-CARBON SUGARS IN EUCALYPTUS EXTRACTION LIQUOR
THE RELATIONSHIP BETWEEN TWO METHODS FOR EVALUATING FIVE-CARBON SUGARS IN EUCALYPTUS EXTRACTION LIQUOR Congcong Chi, a,b* Zeng Zhang, a Weiwei Ge, a and Hasan Jameel b Alkaline pre-extraction and hydrothermal
More informationThe Use of Novel Enzyme Accelerant Technology in Reducing Costs and Increasing Yields in Ethanol Production.
The Use of Novel Enzyme Accelerant Technology in Reducing Costs and Increasing Yields in Ethanol Production. Ken Matthews. Business Development Manager Eka Chemicals Inc. 1 Pulp, Paper and More. The Use
More informationAppearance Celluclast 1.5 L FG is a brown liquid with a density of approximately 1.2 g/ml. Celluclast 1.5 L FG EGU/g.
Page 1:5 Special Food / 2001-08524-03.pdf Product Sheet Celluclast 1.5 L FG Description Celluclast 1.5 L FG is a liquid cellulase preparation produced by submerged fermentation of a selected strain of
More informationOptimization of saccharification conditions of prebiotic extracted jackfruit seeds
Paper Code: fb005 TIChE International Conference 0 November 0, 0 at Hatyai, Songkhla THAILAND Optimization of saccharification conditions of prebiotic extracted jackfruit seeds Sininart Chongkhong *, Bancha
More informationDilute Acid Pretreatment of Corncob for Efficient Sugar Production
DOI 10.1007/s12010-010-9071-4 Dilute Acid Pretreatment of Corncob for Efficient Sugar Production G. S. Wang & Jae-Won Lee & J. Y. Zhu & Thomas W. Jeffries Received: 3 May 2010 / Accepted: 16 August 2010
More informationLAP-003CS. Procedure Title: Author(s): Bonnie Hames, Fannie Posey-Eddy, Chris Roth, Ray Ruiz, Amie Sluiter, David Templeton.
Biofuels Program Biomass Analysis Technology Team Laboratory Analytical Procedure LAP-003CS Procedure Title: Determination of Acid-Insoluble Lignin in Corn Stover Author(s): Bonnie Hames, Fannie Posey-Eddy,
More informationAPPLICATION OF SUPERCRITICAL FLUIDS FOR POLYPHENOLIC COMPOUNDS EXTRACTION FROM EXHAUSTED OLIVE POMACE
Processes APPLICATION OF SUPERCRITICAL FLUIDS FOR POLYPHENOLIC COMPOUNDS EXTRACTION FROM EXHAUSTED OLIVE POMACE Ashley Sthefanía Caballero 1, Juan Miguel Romero-García 2, Eulogio Castro 2, Carlos Ariel
More informationDegradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm
Bioresource Technology 96 (5) 1357 1363 Degradation of lignin in pulp mill wastewaters by white-rot fungi on biofilm Juan Wu a,b, Ya-Zhong Xiao b, Han-Qing Yu a, * a Laboratory of Environmental Engineering,
More informationMass flow every 24h BCRL SSCF. Enzymes Biomass, Water EH. Liquid Ferm. broth
Fig. S1 BCRL SHF Mass flow every 24h Enzymes Biomass, Water EH hydrolysate Ferm. Ferm. broth Enzymes Biomass, Water EH Whole hydrolysate SSCF Ferm. broth Solids buildup Solids buildup BCRL SHF EH (ml)
More informationComparative Study of Bioethanol Production from Cassava Peels by Monoculture and Co-Culture of Yeast
Kasetsart J. (Nat. Sci.) : - 7 (11) Comparative Study of Bioethanol Production from Cassava Peels by Monoculture and Co-Culture of Yeast Jirasak Kongkiattikajorn* and Buddhiporn Sornvoraweat ABSTRACT The
More informationKINETIC MODELING OF ENZYMATIC HYDROLYSIS OF POPLAR WASTE BY WET OXIDATION PRETREATMENT
KINETIC MODELING OF ENZYMATIC HYDROLYSIS OF POPLAR WASTE BY WET OXIDATION PRETREATMENT Shanshan Liu, a Guigan Fang, a, * Qiang Wang, b Yongjun Deng, a and Shanming Han a Kinetic modeling of enzymolysis
More informationUSE OF ENZYMES IN HYDROLYSIS OF MAIZE STALKS. Ivo Valchev, Sanchi Nenkova, Petya Tsekova, and Veska Lasheva
USE OF ENZYMES IN HYDROLYSIS OF MAIZE STALKS Ivo Valchev, Sanchi Nenkova, Petya Tsekova, and Veska Lasheva Lignocellulosic biomass is the most abundant organic raw material in the world. Cellulose and
More informationPRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY MUSHROOMS
PRODUCTION OF LIGNOCELLULOLYTIC ENZYMES BY MUSHROOMS PETR BALDRIAN Laboratory of Environmental Microbiology, Institute of Microbiology of the ASCR, v.v.i., Videnska 1083, 14220 Praha 4, Czech Republic,
More informationConversion of glycerol to ethanol and formate by Raoultella Planticola
Conversion of glycerol to ethanol and formate by Raoultella Planticola Li Z.A.D 1., Chong W.K., Mathew, S., Montefrio, M.J.F. and Obbard J.P. 2 Division of Environmental Science and Engineering, National
More informationLactic acid production from rice straw using plant-originated Lactobacillus rhamnosus PN04
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(5):590-594 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Lactic acid production from rice straw using plant-originated
More informationARABINAN
www.megazyme.com ARABINAN ASSAY PROCEDURE K-ARAB 08/18 (100 Assays per Kit) Megazyme 2018 INTRODUCTION: In the processing of apples and pears, the yield of juice can be dramatically improved by using enzymes
More informationEnzymatic Hydrolysis of Oil Palm Empty Fruit Bunch using Membrane Reactor
1543 A publication of CHEMICAL ENGINEERING TRANSACTIONS VOL. 56, 2017 Guest Editors: Jiří Jaromír Klemeš, Peng Yen Liew, Wai Shin Ho, Jeng Shiun Lim Copyright 2017, AIDIC Servizi S.r.l., ISBN 978-88-95608-47-1;
More informationMonosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail
Monosaccharides and Ethanol Production from Superfine Ground Sugarcane Bagasse Using Enzyme Cocktail Jingbo Li, Pengfei Zhou, Hongmei Liu, Jianghai Lin, Yingxue Gong, Wenjuan Xiao, and Zehuan Liu* In this
More informationMIXED XYLANASE, β-glucanase ENZYME PREPARATION, produced by a strain of HUMICOLA INSOLENS
MIXED XYLANASE, β-glucanase ENZYME PREPARATION, produced by a strain of HUMICOLA INSOLENS New specifications prepared at the 61st JECFA (2003) and published in FNP 52 Add 11 (2003). An ADI not specified
More informationThe biochemistry of wood degradation. Kari Steffen
The biochemistry of wood degradation Kari Steffen verview Degradation of dead wood focuses on fungal activity Enzymatic attack of natural biopolymers The main organic components of dead wood Cellulose
More informationEFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-110
BIOLOGIA 21, 47 (1&2), PP 59 65 ISSN 6 396 EFFECT OF ADDITIONAL MINERAL IONS ON CITRIC ACID PRODUCTIVITY BY ASPERGILLUS NIGER NG-11 SIKANDER ALI 1, IKRAM-UL-HAQ 1 AND JAVED IQBAL 2 1 Biotechnology Research
More informationAnaerobic fermentation of organic wastes for production of soluble organic compounds
Anaerobic fermentation of organic wastes for production of soluble organic compounds Barış Çallı Marmara University Department of Environmental Engineering Istanbul, Turkey BioPXenoR Workshop, October
More informationHeterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as a Carbon Substrate
2011 International Conference on Food Engineering and Biotechnology IPCBEE vol.9 (2011) (2011)IACSIT Press, Singapoore Heterotrophic Growth of Chlorella sp. KKU-S2 for Lipid Production using Molasses as
More informationThe Application of Détente Instantanée Contrôlée (DIC) Technology to Minimize the Degradation Rate of Glucose
International Proceedings of Chemical, Biological and Environmental Engineering, Vol. 88 (2015) DOI: 10.7763/IPCBEE. 2015. V88. 6 The Application of Détente Instantanée Contrôlée (DIC) Technology to Minimize
More informationMANNOSYLERYTHRITOL LIPIDS (MEL) AS ADDITIVES IN COSMETIC FORMULATIONS
MANNOSYLERYTHRITOL LIPIDS (MEL) AS ADDITIVES IN COSMETIC FORMULATIONS Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB Alexander Beck SuperBIO Workshop Biosurfactants, Gent, Belgium
More informationRedefine the Role of Lignin in Enzymatic Hydrolysis of Lignocellulosic Biomass
Redefine the Role of Lignin in Enzymatic Hydrolysis of Lignocellulosic Biomass Maobing Tu Auburn University Background Biomass pretreatment is needed Break down the recalcitrant structure of cell walls
More informationBiolignin, a renewable and efficient material for wood adhesives
Biolignin, a renewable and efficient material for wood adhesives Dr. Bouchra Benjelloun-Mlayah, Dr. Nadine Tachon, Dr. Louis Pilato and Prof. Dr. Michel Delmas 53th SWST Conference Zvolen, Slovaquia, June
More informationProduction of Cellulase from Aspergillus fumigatus Under Submerged and Solid State Fermentation Using Agricultural Waste
INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACY, BIOLOGY AND CHEMISTRY Research Article Production of Cellulase from Aspergillus fumigatus Under and Solid State Using Agricultural Waste P. Shobana and N.
More informationMODIFICATION OF FORAGE QUALITY POST-HARVEST
MODIFICATION OF FORAGE QUALITY POST-HARVEST http://www.hayhorsefeeders.com/round-bale-feeders.html Gwinyai E. Chibisa, Ph.D. Chemical Treatments: Hydrolytic Agents Hydrolytic agents = Alkali s e.g., NaOH,
More informationMathematical Modeling for the Prediction of Liquid Glucose and Xylose Produced From Cassava Peel
American Journal of Engineering Research (AJER) e-issn: 232-847 p-issn : 232-936 Volume-6, Issue-5, pp-274-28 www.ajer.org Research Paper Open Access Mathematical Modeling for the Prediction of Liquid
More informationScholars Research Library. Purification and characterization of neutral protease enzyme from Bacillus Subtilis
Journal of Microbiology and Biotechnology Research Scholars Research Library J. Microbiol. Biotech. Res., 2012, 2 (4):612-618 (http://scholarsresearchlibrary.com/archive.html) Purification and characterization
More informationVALUE-ADDITION TO CORN-ETHANOL STILLAGE USING OLEAGINOUS MUCOR CIRCINELLOIDES
VALUE-ADDITION TO CORN-ETHANOL STILLAGE USING OLEAGINOUS MUCOR CIRCINELLOIDES Debjani Mitra Iowa State University Lawrence Berkeley Laboratory 5 th June, 2012 Corn Utilization & Technology Conference,
More informationBio-delignification ability of locally available edible mushrooms for the biological treatment of crop residues
Indian Journal of Biotechnology Vol 11, April 2012, pp 191-196 Bio-delignification ability of locally available edible mushrooms for the biological treatment of crop residues Ch Vijya 1 and R Malikarjuna
More informationProduction of thermostable lignolytic enzymes by Thermoascus aurantiacus MTCC 375 using paddy straw as substrate
ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 116-121 http://www.ijcmas.com Original Research Article Production of thermostable lignolytic enzymes by Thermoascus aurantiacus MTCC 375 using paddy straw
More informationComparative evaluation of some brown midrib sorghum mutants for the production of food grain and 2,3-butanediol
Comparative evaluation of some brown midrib sorghum mutants for the production of food grain and 2,-butanediol Yadhu N Guragain 1, K.S. Vinutha 2, G.S. Anil Kumar 2, Reggeany Barrios 1, P.V. Vara Prasad,
More informationEnzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol
An- Najah National University Faculty of Graduate Studies Enzymatic Hydrolysis of Olive Industry Solid Waste into Glucose, the Precursor of Bioethanol By Israa Jamal Mohammad Dagher Supervisor Prof. Shehdeh
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationCOMPARATIVE STUDY OF DIFFERENT METHODS OF HYDROLYSIS AND FERMENTATION FOR BIOETHANOL OBTAINING FROM INULIN AND INULIN RICH FEEDSTOCK
Studii şi Cercetări Ştiinţifice Chimie şi Inginerie Chimică, Biotehnologii, Industrie Alimentară Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry 2012, 13 (1),
More informationLignin Isolation from Pulp
Lignin Isolation from Pulp Several different enzymatic, chemical and mechanical methods have been developed for the isolation of lignin from wood and pulp. However, due to the heterogeneous nature of wood
More informationHydrolysis and Fractionation of Hot-Water Wood Extracts
C Hydrolysis and Fractionation of Hot-Water Wood Extracts Thomas E. Amidon Christopher D. Wood, Jian Xu, Yang Wang, Mitchell Graves and Shijie Liu Biorefinery Research Institute Department of Paper and
More informationEnsiling as a method to preserve energy crops and to enhance the energy yields Seija Jaakkola (UH) Ensiling
Ensiling as a method to preserve energy crops and to enhance the energy yields Seija Jaakkola (UH) Ensiling Fred Stoddard (UH) Energy crops, cultivations Maritta Kymäläinen (HAMK) Biogas Pekka Maijala,
More informationThe Contribution of Enzymes to Bioprocessing and Industrial Sustainability
The Contribution of Enzymes to Bioprocessing and Industrial Sustainability Ghent, Belgium September 21st 2005 Kirsten Birkegaard Stær, Director External Affairs Novozymes in brief Danish biotech-based
More informationHEMICELLULASE from ASPERGILLUS NIGER, var.
HEMICELLULASE from ASPERGILLUS NIGER, var. Prepared at the 55th JECFA (2000) and published in FNP 52 Add 8 (2000), superseding tentative specifications prepared at the 31st JECFA (1987) and published in
More informationEvaluation of pine kraft cellulosic pulps and fines from papermaking as potential feedstocks for biofuel production
Cellulose (2016) 23:649 659 DOI 10.1007/s10570-015-0808-7 ORIGINAL PAPER Evaluation of pine kraft cellulosic pulps and fines from papermaking as potential feedstocks for biofuel production Kamila Przybysz
More informationJOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.625, ISSN: , Volume 2, Issue 11, December 2014
COMPARISON OF ACTIVITY OF PECTINASE USING AGRICULTURAL WASTE SUBSTRATES A STUDY M.P.KUSUMA* DR M.V.V.CHANDANA LAKSHMI** *Assistant Professor, RBVRR College of Pharmacy, Osmania University, India **Associate
More informationTHE EFFECT OF PRETREATMENT AND VARIETY OF MICROORGANISMS TO THE PRODUCTION OF ETHANOL FROM COFFEE PULP
THE EFFECT OF PRETREATMENT AND VARIETY OF MICROORGANISMS TO THE PRODUCTION OF ETHANOL FROM COFFEE PULP Tri Widjaja 1, Ali Altway 1, Siti Nurkhamidah 1, Luluk Edahwati 2, Fibrillian Zata Lini 1 and Fixalis
More informationRenewable Carbon-Feedstock to Industrial Chemicals: Producing Renewable Materials from Granular Starch
S E 1 E 2 E 3 E 4 E n Continuous Biocatalytic Systems P Renewable Carbon-Feedstock to Industrial Chemicals: Producing Renewable Materials from Granular Starch Manoj Kumar, PhD Sr. Scientist, Research &
More informationFACTORS AFFECTING THE GROWTH OF MICRO-ORGANISMS IN FOODS
FACTORS AFFECTING THE GROWTH OF MICRO-ORGANISMS IN FOODS FACTORS AFFECTING THE GROWH OF MICRO-ORGANISMS IN FOODS Several factors related to the environment and the conditions in which food is stored influence
More informationChapter IV. Optimization of Cultural Conditions for Decolorization of Textile Dye Effluent
Chapter IV Optimization of Cultural Conditions for Decolorization of Textile Dye Effluent The ability of the fungal system to degrade azo dyes depends on the structural characteristics of the dyes, temperature,
More informationBiodegradation of coffee husk substrate during the mycelia growth of Pleurotus ostreatus and the effect on in vitro digestibility.
Biodegradation of coffee husk substrate during the mycelia growth of Pleurotus ostreatus and the effect on in vitro digestibility Irma Badarina 1, D. Evvyernie 1, T. Toharmat 1, E. N. Herliyana 2 & L.K.
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 211, 1 (3):124-129 ISSN: 2248 9215 Production of Alkaline Protease by Bacillus subtilis (MTCC7312) using Submerged
More informationNew Approaches for Improving Edible Oil Quality
New Approaches for Improving Edible Oil Quality Selma Turkay and Hale Gurbuz Istanbul Technical University, Chemical Engineering Department OFI TURKEY 2014 13 May Istanbul Introduction In recent years,
More informationBioresource Technology
Bioresource Technology 102 (2011) 11115 11120 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Short Communication Effects of enzyme loading
More informationEffect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S.
Available online at www.sciencedirect.com APCBEE Procedia 00 (2012) 000 000 Conference title Effect of Different Combinations of Soybean and Wheat Bran on Enzyme Production from Aspergillus oryzae S. Chuenjit
More informationENZYMATIC DIGESTIBILITY OF TOMATO, PEPPER, AND EGGPLANT STALKS MIXTURE
ENZYMATIC DIGESTIBILITY OF TOMATO, PEPPER, AND EGGPLANT STALKS MIXTURE Yalçın Çöpür, a, * Ömer Özyürek, a Ayhan Tozluoglu, and Selva Kütük Turkey annually produces 26 million tons of vegetables and is
More informationOligosaccharide Hydrolysis in Plug Flow Reactor using Strong Acid Catalyst Young Mi Kim, Nathan Mosier, Rick Hendrickson, and Michael R.
Oligosaccharide Hydrolysis in Plug Flow Reactor using Strong Acid Catalyst Young Mi Kim, Nathan Mosier, Rick Hendrickson, and Michael R. Ladisch Laboratory of Renewable Resources Engineering Department
More informationOptimizing the Conversion of Pretreated Sila Sorghum Stalks to Simple Sugars Using Immobilized Enzymes
Optimizing the Conversion of Pretreated Sila Sorghum Stalks to Simple Sugars Using Immobilized Enzymes Wiseman Tumbo Ngigi Department of Chemical & Process Engineering, Moi University, P.O. Box 39-31,
More informationThe effect of dilute-acid pretreatment on cellulose crystallinity and digestibility
The effect of dilute-acid pretreatment on cellulose crystallinity and digestibility Name course : Thesis project Biobased Chemistry and Technology Number : BCT-80324 Study load : 24 ects Date : 13-01-2016
More informationBioenergy and Resource Management Centre Cranfield University, UK
Bio-based Production of Platform Chemical 3-Hydroxypropanoic Acid Dr Vinod Kumar Lecturer in Bioenergy/Biomass Systems 25 th October 2017 Bioenergy and Resource Management Centre Cranfield University,
More informationBicomponent films of cellulose and lignin and their biodegradation
LIGNOCELLVALUE-ADDED MATERIALS AND FUNCTIONAL STRUCTURES FROM LIGNOCELLULOSICS Bicomponent films of cellulose and lignin and their biodegradation Steering group meeting 20.5.2011 Ingrid Hoeger Outline
More informationEnhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment
Enhancement of total sugar and lignin yields through dissolution of poplar wood by hot water and dilute acid flowthrough pretreatment Yan et al. Yan et al. Biotechnology for Biofuels 2014, 7:76 Yan et
More informationSyringe Pump Application Note AN27. Figure 1: Phase diagram of water showing vapor-liquid relationship for subcritical water
Measurement of Aqueous Solubility of Compounds at High Temperature Using a Dynamic Flow Apparatus and a Teledyne Isco Syringe Pump Jerry W. King & Keerthi Srinivas, University of Arkansas, Dept. of Chemical
More informationCanadian Journal of Microbiology
Recovery of glucose from dried distiller's grain with solubles using combinations of solid-state fermentation and insect culture Journal: Canadian Journal of Microbiology Manuscript ID cjm-2018-0042.r2
More informationOptimization of Trichoderma reesei Medium for Increasing Xylanase Enzyme Production
Advances in Bioresearch Adv. Biores., Vol 7 (3) May 2016: 94-99 2015 Society of Education, India Print ISSN 0976-4585; Online ISSN 2277-1573 Journal s URL:http://www.soeagra.com/abr.html CODEN: ABRDC3
More informationEnzyme Applications in Pulp and Paper Manufacturing
Enzyme Applications in Pulp and Paper Manufacturing Mike Paice Lakehead University Symposium September 27, 2005 2005 Paprican 1 utline Background on status of biotech in P&P Enzymes in Kraft pulping Enzymes
More information