Multicenter Analytical Evaluation of an Automated Immunoassay for Total Plasma Homocysteine

Size: px
Start display at page:

Download "Multicenter Analytical Evaluation of an Automated Immunoassay for Total Plasma Homocysteine"

Transcription

1 Annals o f Clinical & Laboratory Science, vol. 30, no. 2, Multicenter Analytical Evaluation of an Automated Immunoassay for Total Plasma Homocysteine Alan H.B. W u,1 Verena H oltm an,1 Fred S. A p p let Vincent Ricchiuti,2 Patricia M. DiBello,3 and Donald Jacobsen3 1 Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, Connecticut 2 Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, Minneapolis, Minnesota 3 Cleveland Clinic Foundation, Cleveland, Ohio Abstract. A fully automated immunoassay for total plasma homocysteine assay was evaluated at four centers. To measure total homocysteine, oxidized forms of homocysteine in serum and plasma were reduced by dithiothreitol and assayed by a competitive fluorescence polarization technique. The assay had within-run precision from 0.9 to 3.0% and total precision from 2.8 to 4.1% for control materials with homocysteine concentrations of approximately 7, 12.5, and 25 JLmol/L, a sensitivity of 0.35 lmol/l, good parallelism upon dilution, and analytical recovery ranging from 97.4 to 103.8%. The immunoassay correlated with four different HPLC assays for homocysteine, yielding a slope of 0.98, an intercept of jlmol/l, and a correlation coefficient of for 440 paired samples. The reference range, determined with plasma samples from 609 males and 600 females, yielded a mean of 9.17i2.86 (Omol/L, with a central 95% range of (Xmol/L. The immunoassay is a suitable alternative to HPLC and may be useful in screening persons with high risk of coronary artery disease. Keywords: Plasma homocysteine, fluorescence polarization immunoassay, cardiovascular disease Introduction Hom ocysteine is a dem ethylated product of methionine metabolism and circulates in the plasma in reduced and oxidized forms; the oxidized forms include the disulfide, homocystine, and mixed disulfides containing homocysteine, cysteine, and protein. Total homocysteine (thcy) is a strong independent risk factor for cardiovascular disease [1,2]. Thus, elevated total plasma homocysteine is associated with atherosclerosis [3], stroke [4], peripheral vascular disease [5], venous thrombosis [6], and end-stage renal disease [7]. The causes of hyperhomocysteinemia in subjects with cardiovascular disease are poorly understood and multifactorial, involving genetic and acquired components [8-11]. Address correspondence to Alan H.B. Wu, Ph.D., Hartford Hospital, 80 Seymour St., Hartford, CT 06102; tel ; fax ; awu@harthosp.org. During the past several years, various assays have been developed to measure thcy [12]. These are based on, but not limited to, gas chromatography/mass spectrom etry [13], high pressure liquid chrom atography (HPLC) with electrochemical detection [14], and HPLC with fluorescence detection [15-17]. Although there is generally good agreement among the assays, most are labor intensive and have relatively low throughput. Recently, immunoassays for thcy were introduced [18,19]. In the present study, a fully automated commercial immunoassay with relatively high throughput, using the Abbott IMx analyzer, has been evaluated in four different laboratories by comparisons with established HPLC methods for thcy. Materials and M ethods This study was performed at four laboratories: Hennepin County Medical Center, Minneapolis, MN; /00/ $1.50; 2000 by the Association of Clinical Scientists, Inc.

2 186 Annals o f Clinical dr Laboratory Science Cleveland Clinic Foundation, Cleveland, OH; Oregon Regional Primate Research Center, Beaverton, OR; and Hartford Hospital, Hartford, CT. Samples provided by these sites were serum or plasma (EDTA or lithium heparin), which represented fresh surplus samples that remained after routine analyses or frozen samples that remained from previous analyses. Enrollment of subjects followed protocols that were approved by the Institutional Review Board at each institution. Criteria for enrollment for the normal range study varied from site to site. Age and gender were recorded for >99% of the patients (age information was missing for four samples); the type of collection tube was also recorded. One site excluded volunteers who had a history of coronary artery disease, and one site included outpatients without evidence of disease and with a normal chemistry profile (including lipids and lipoprotein) that did not include specific screening tests for cardiac disease. The two remaining sites used samples from general hospital populations (outpatients and inpatients) which had homocysteine results in the normal range, based on HPLC analyses. For correlation studies, elevated samples were obtained from high risk patients, patients undergoing methionine load testing, and samples known to be elevated from previous HPLC testing. Plasma samples were processed within 20 min of collection. Serum samples were collected in SST tubes and centrifuged within 1 hr. All samples were stored frozen (< -20 C) until assay by the Abbott IMx technique. Samples were tested within 3 mo of storage. For correlation studies, samples were frozen between HPLC and IMx analyses. For the reference range, study site #1 collected 225 serum, 62 EDTA plasma, and 2 lithium heparin plasma samples; site #2 collected 320 EDTA samples; site #3 collected 2 serum and 298 EDTA samples; and site #4 collected 300 serum samples. For the correlation study, site #1 collected 48 serum, 5 EDTA, and 65 heparin samples; site #2 collected 80 EDTA samples; site #3 collected 176 EDTA samples; and site #4 collected 66 serum samples. All sites were provided with IMx homocysteine reagents, calibrators, and controls (Abbott Laboratories, Abbott Park, IL) and the samples for precision studies. The sites provided the samples for correlation with HPLC assays, the normal range study, and the dilution recovery. IM x method. The IMx homocysteine assay is a quantitative, competitive fluorescence polarization im m unoassay (FPIA) which measures total homocysteine (free, bound, oxidized, reduced) in human serum or plasma (EDTA or heparin). The assay method has been described previously [18,20] and is based on the enzymatic conversion of homocysteine to S-adenosyl-homocysteine. The assay utilizes six S- adenosyl-l-homocysteine calibrators (0,2.5, 5.0,10.0, 20.0, and 50.0 Jmol/L) prepared in a buffered solution. The calibration curve is stored and may be used for a minimum of two weeks. Three assay controls (7.0, 12.5, and 25.0 (Xmol/L) are composed of L- homocystine added to a serum-based matrix. Precision. Assay precision was assessed at each site over five days and at two sites over twenty days, according to a modified NCCLS protocol, using the three serumbased controls. Additionally, precision of the automated dilution protocol (1:10) was compared to a manual 1:10 dilution using two L-homocysteinespiked serum samples and the modified five-day NCCLS protocol. Analytical sensitivity. Analytical sensitivity was determined by testing ten replicates of IMx calibrator A (0.0 lmol/l) followed by calibrator B (2.5 fimol/l) in replicates of two. The sensitivity of the IMx homocysteine assay was calculated by determining the concentration (jimol/l) corresponding to two standard deviations from the mean of the rate of calibrator A. Dilution linearity. Five serum samples with elevated total homocysteine concentrations were serially diluted and assayed in duplicate at each site. The sites were requested to analyze two samples with thcy value between 50 and 100 j_lmol/l and three samples between 20 and 50 imol/l. Linear regression analysis of the observed homocysteine concentration was performed as a function of the expected concentration. Statistical analyses. Data were analyzed using SAS computer software, version 6.09, on a Unix operating system. Linear regression was performed using proc reg, which is a least-squares linear regression analysis. The statistical method for establishing the reference range was proc capability, which uses the 2.5 and

3 Evaluation o f the IM x assay for plasma homocysteine percentiles of each sample category to represent the category s expected 95% range of values. borohydride for reduction and HPLC separation with electrochemical detection [14]. Reference range. Samples from approximately 150 males and 150 females were assayed at each site to determine the reference range for thcy using the IMx assay. Samples were collected in serum, lithium heparin, or EDTA plasma tubes. Specimen history forms were completed to record the specimen collection tubes, plus each participant s age and gender. Samples were stored refrigerated (2-8 C) for < 3 days before analysis, or frozen at 20 C for < 6 months before analysis. A total of 1209 samples were tested. Correlation with HPLC. Specimens with thcy values spanning the dynamic range of the IMx assay (0 50 Omol/L) were assayed by both IMx and HPLC. Linear regression analysis was performed with the HPLC assay (x-axis) versus the IMx assay (y-axis) at each site. The four sites used three different HPLC methods. Sites #1 and #4 used tri-n-butylphosphine for reduction, derivitization with 7-fluorobenzo-furazane-4-sulfonic acid, and separation by HPLC [16,21]. Site #2 used sodium borohydride reduction, derivitization with monobromobimane, and HPLC separation with fluorescence detection [17]. Site #3 used sodium Results At the four sites, the precision of the IMx assay for thcy over 5 days ranged from 0.9 to 3.0%, and for thcy over 20 days from 2.8 to 4.1%, for the three control levels. The precision of the automated dilution ranged from 1.9 to 3.1%, compared to the manual dilution, which ranged from 1.7 to 9.9%. The grand means and coefficients of variation (CV) across all sites and reagent lots are shown in Table 1. There were no significant differences among the four sites in analytical precision, based on total CV percentages calculated across the sites (data not shown). The data from 12 runs using two different reagent lots (4 runs with lot A and 8 runs with lot B) were used to calculate analytical sensitivity. The analytical sensitivity was determined to be 0.35 jimol/l. Twenty samples were serially diluted to a final concentration of approximately 5 Umol/L, which corresponded to endpoint dilution factors ranging from Table 2. Homocysteine concentrations in a reference population, categorized by age and gender. Table 1. Precision studies of the IMx homocysteine assay.a Age (yr) & gender N Plasma homocysteine mean ±SD conc ( Jmol/L) log mean Hog SD Control N Mean homocysteine concentration Umol/L Within-run precision data SD %CV Total precision data SD %CV Low Medium High Auto l b Auto 2b Manual Ie Manual 2C a n = 80 for the 5-day study; n =1 6.0 for the 20 day study, k Auto = automated dilution, 1 & 2 are different samples. c Manual = manual dilution, 1 & 2 are different samples. females ± males

4 188 Annals o f Clinical & Laboratory Science 1:4 to 1:16. The recovery values ranged from 97.4% to 103.8%. Linear regression analysis of the observed thcy concentration vs the expected concentration showed linearity (r = , slope = ). The mean thcy concentration in the studied population (n = 1209) was 9.17 imol/lwith a central 95% range of lmol/l; the population distribution was skewed to the right and upon logarithmic transformation was normally distributed (Fig. 1). The mean thcy concentration for females (8.50 jlmol/l, central 95% range Jmol/L) was significantly lower than the mean th cy concentration for males (9.82 jlmol/l, central 95% range lmol/l). The data, analyzed by age and sex (n = 1205), showed an increase in thcy levels with increasing age, with the values for males consistently greater than the corresponding values for females in each age group. The population data are summarized in Table 2. Each site s correlation data for IMx vs HPLC assays are summarized in Table 3. Fig. 2 shows the combined correlation data. Overall, the correlation between HPLC and IMx was 0.966, with a slope of 0.98 and intercept of-0.19 fimol/l. The differences in slope at the various sites can be attributed to the different methods of assay, calibration, and standardization. Discussion Modern analytical methods for the measurement of th cy are largely based on some form of chromatography [13-17]. These methods measure total homocysteine concentration, which comprises the sum of the reduced and oxidized species. The disadvantages of some chromatographic techniques are that they are laborious, time consuming, and not amenable to high volumes of testing or screening of the general population. An enzyme-linked immunoassay has been developed using a microtiter plate format [19,22]. Although this assay compares well to the results by HPLC assay, semi-automated analysis requires m icrotiter plate washers and readers that are uncommon in clinical laboratories in the USA. The H C Y nmol/l I Log H C Y im ol/l Fig. 1. Frequency distribution graphs for plasma homocysteine concentrations in the reference population. In panel A, the abscissa has an arithmetic scale; in panel B, the abscissa has a logarithmic scale.

5 Evaluation o f the IM x assay for plasma homocysteine 189 development of the first fully automated assay for thcy facilitates the routine availability of this assay. A prototype assay of the current system demonstrated excellent precision, calibration curve stability, parallelism, recovery, and correlation with chromatographic methods [18], and the precision of this automated method was equal or superior to other published assays [17,19,21]. In this report, use of the FDA-approved IMx homocysteine assay was validated in a multi-center clinical study. The availability of results on an automated basis enabled verification of the reference range on a large population (n = 1209). Although different assays were used, results in this study were correlated with other reference range reports, as summarized by Ueland et al [23]. Previous studies of paired serum and plasma samples showed significant differences [14,17] due to the synthesis and release of homocysteine from red cells while the serum sample was clotting at room temperature. The preferred sample is EDTA plasma that is either centrifuged immediately to remove the red cells, or stored on ice until centrifugation. Storage of the sample on ice slows the synthesis of the homocysteine, and previous studies have dem onstrated no significant increase in homocysteine in EDTA plasma samples stored on ice for 8 hr [20]. Since this study used excess samples from previous studies, we were unable to collect matched serum and plasma samples. Use of an autom ated assay also provides a convenient means for screening homocysteine in high risk patients, as some have recently recommended [24]. Prospective studies are being conducted to determine if lowering plasma homocysteine concentrations is attended by decreased risks of cardiovascular, peripheral vascular, or cerebrovascular diseases. If such correlation is evident, routine screening may be warranted. Acknowledgments This study was supported by Abbott Laboratories, Abbott Park, IL, USA, and by Axis Biochemicals ASA, Oslo, Norway. Table 3. Correlation statistics for plasm a hom ocysteine concentrations assayed by the IMx and HPLC techniques at the four laboratory sites. Lab Site Homocysteine concentration range by IMx ( Imol/L) N r Slope o f the regression line (95% Cl) [and its y-intercept (95% Cl)] ( )] [0.12 ( )] ( ) [0.17 ( )] ( ) [0.21 ( )] Homocysteine conc. by HPLC assay (fxmol/l) Fig. 2. Combined correlation graph o f plasma homocysteine concentrations assayed by the IMx and HPLC techniques (square = site 1; circle = site 2; inverted Y = site 3; asterisk = site 4) ( ) [1.93 ( )] All ( ) [-0.19 ( )]

6 190 Annals o f Clinical & Laboratory Science References 1. Boushey CJ, Beresford SAA, Omenn GS, Motulsky AG. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease probably benefits of increasing folic acid intakes. J Am Med Assoc 1995; 274: Refsum H, U eland P, Nygärd O, Vollset SE. Homocysteine and cardiovascular disease. Ann Rev Med 1998;49: Mayer EL, Jacobsen DW, Robinson K. Homocysteine and coronary atherosclerosis. J Am Coll Cardiol 1996; 27: Coull BM, Malinow MR, Beamer H, Sexton G, Nordt F, de Garmo P. Elevated plasma homocyst(e)ine concentration as a possible independent risk factor for stroke. Stroke 1990;21: Taylor LM Jr, Moneta GL, Sexton GJ, SchuffRA, Porter JM, and the Homocysteine and Progression o f Atherosclerosis Study Investigators. Prospective blinded study of the relationship between plasma homocysteine and progression of symptomatic peripheral arterial disease. J Vase Surg 1999;29: DAngelo A, Selhub J. Homocysteine and thrombotic disease. Blood 1997;90: Bostom AG, Lathrop L. Hyperhomocysteinemia in end-stage renal disease: prevalence, etiology, and potential relationship to arteriosclerotic outcomes. Kidney Int 1997;52: Selhub J, Jacques PF, Wilson PWF, Rush D, Rosenberg IH. Vitamin status and intake as primary determinants of homocysteinemia in an elderly population. J Am Med Assoc 1993;270: Nygard O, Vollset SE, Refsum H, Stensvold I, Tverdal A, Nordrehaug JE, Ueland M, Kvale G. Total plasma homocysteine and cardiovascular risk profile the Hordaland homocysteine study. J Am Med Assoc 1995; 274: Green R, Jacobsen DW. Clinical implications of hyperhomocysteinemia. In: Folate in Health and Disease (Bailey LB, ed), Marcell Dekker, Inc, New York, 1995; pp Nygard O, Refsum H, Ueland PM, Vollset SE. Major lifestyle determinants of plasma total homocysteine distribution: the Hordaland homocysteine study. Am J Clin N utr 1998;67: Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH. Total homocysteine in plasma or serum: Methods and clinical applications. Clin Chem 1993;39: Stabler SP, M arcel PD, Podell ER, Allen RH. Quantitation of total homocysteine, total cysteine, and methionine in normal serum and urine using capillary gas chromatography-mass spectrometry. Anal Biochem 1997;162: Malinow MR, Kang S-S, Taylor LM, Wong PW, Coull B, Inahara T, Mukerjee D, Sexton G, Upson B. Prevalence of hyperhomocyst(e)inemia in patients with peripheral arterial occlusive disease. Circulation 1989; 79: Refsum H, Ueland PM, Svardal AM. Fully automated fluorescence assay for determining total homocystine in plasma. Clin Chem 1989;35: Vester B, Rasmussen K. High performance liquid chromatography m ethod for rapid and accurate determination of homocysteine in plasma and serum. Eur J Clin Chem Clin Biochem 1991;29: Jacobsen DW, Gatautis VJ, Green R, Robinson K, Savon SR, Seek M, Ji J, Otto JM, Taylor LM Jr. Rapid HPLC determination of total homocystine and other thiols in serum and plasma: sex differences and correlation with cobalamin and folate levels in normal subjects. Clin Chem 1994;40: Shipchandler MT, Moore EG. Rapid, fully automated measurement of plasma homocyst(e)ine with the Abbott IMx analyzer. Clin Chem 1995;41: Frantzen F, Faaren AL, Alfheim I, Nordhei AK. Enzyme conversion immunoassay for determ ining total homocystine in plasma or serum. Clin Chem 1998; 44: Alfheim I, Auerbekk A, Holets-McCormack S, Shih J. Development of an assay for homocysteine on an automated immunoassay analyzer (Abstract). Clin Chem 1998;44(suppl):A Dudman N, Guo X, Crooks R, Xie L, Silberberg J. Assay of plasma homocysteine: light sensitivity of the fluorescent 7-benzo-2-oxa-l,3-diazole-4-sulfonic acid derivative, and use of appropriate calibrators. Clin Chem 1996;42: Pietzsch J, Pixa A. Determination of total homocysteine. Clin Chem 1998;44: Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A, Allen RH. Total homocysteine in plasma or serum: methods and clinical applications. Clin Chem 1993;39: M alinow MR, Bostom AG, Krauss RM. Homocyst(e)ine, diet, and cardiovascular diseases. A statement for healthcare professions from the Nutrition Committee, American Heart Association. Circulation 1999;99:

Original Article Ann Clin Biochem 2000; 37: 194±198

Original Article Ann Clin Biochem 2000; 37: 194±198 Original Article Ann Clin Biochem 2000; 37: 194±198 Evaluation of the Abbott IMx TM uorescence polarization immunoassay and the Bio-Rad enzyme immunoassay for homocysteine: comparison with high-performance

More information

Comparison of Five Automated Serum and Whole Blood Folate Assays

Comparison of Five Automated Serum and Whole Blood Folate Assays Clinical Chemistry / FIVE AUTOMATED FOLATE ASSAYS Comparison of Five Automated Serum and Whole Blood Folate Assays William E. Owen, MT(ASCP), 1 and William L. Roberts, MD, PhD 2 Key Words: Hemolysate;

More information

Homocysteine Determination in Plasma

Homocysteine Determination in Plasma omocysteine Determination in Plasma Bruce Peary Solomon, Ph.D. Chester T. Duda, Ph.D. Bioanalytical Systems, Inc. West Lafayette, IN E-mail: bp@bioanalytical.com Recent publications suggest that high homocysteine

More information

Polymer Technology Systems, Inc. CardioChek PA Comparison Study

Polymer Technology Systems, Inc. CardioChek PA Comparison Study Polymer Technology Systems, Inc. CardioChek PA Comparison Study Evaluation Protocol: Accuracy Precision Clinical Correlation PTS Panels Lipid Panel Test Strips For Use in Comparisons to a Reference Laboratory

More information

Plasma homocysteine concentrations in a Belgian school-age population 1 3

Plasma homocysteine concentrations in a Belgian school-age population 1 3 Plasma homocysteine concentrations in a Belgian school-age population 1 3 Corinne De Laet, Jean-Claude Wautrecht, Daniel Brasseur, Michèle Dramaix, Jean-Marie Boeynaems, Jean Decuyper, and André Kahn ABSTRACT

More information

EliKine Free Thyroxine (ft4) ELISA Kit

EliKine Free Thyroxine (ft4) ELISA Kit EliKine Free Thyroxine (ft4) ELISA Kit Booklet Item NO. KET0005 Product Name EliKine Free Thyroxine (ft4) ELISA Kit ATTENTION For laboratory research use only. Not for clinical or diagnostic use TABLE

More information

Hyperhomocysteinaemia in Black patients with cerebral thrombosis

Hyperhomocysteinaemia in Black patients with cerebral thrombosis Q J Med 1997; 90:635-639 Hyperhomocysteinaemia in Black patients with cerebral thrombosis R. DELPORT 1, J.B. UBBINK\ W.J.H. VERMAAK 1, H. ROSSOUW 1, P.J. BECKER 2 andj. JOUBERT 3 * From the ^Department

More information

Predictors of Change in Plasma Total Cysteine: Longitudinal Findings from the Hordaland Homocysteine Study

Predictors of Change in Plasma Total Cysteine: Longitudinal Findings from the Hordaland Homocysteine Study Clinical Chemistry 49:1 113 120 (2003) Lipids, Lipoproteins, and Cardiovascular Risk Factors Predictors of Change in Plasma Total Cysteine: Longitudinal Findings from the Hordaland Homocysteine Study Lina

More information

Screening for vitamin B-12 and folate deficiency in older persons 1 3

Screening for vitamin B-12 and folate deficiency in older persons 1 3 Screening for vitamin B-12 and folate deficiency in older persons 1 3 Robert Clarke, Helga Refsum, Jacqueline Birks, John Grimley Evans, Carole Johnston, Paul Sherliker, Per M Ueland, Joern Schneede, Joseph

More information

1 PROTOCOL. Comparison Study Summary. Springs Memorial Hospital. Springs Memorial Hospital 800 W. Meeting St. Lancaster, SC (803)

1 PROTOCOL. Comparison Study Summary. Springs Memorial Hospital. Springs Memorial Hospital 800 W. Meeting St. Lancaster, SC (803) Comparison Study Summary Springs Memorial Hospital 800 W. Meeting St. Lancaster, SC 29720 (803) 286-1480 February 10, 2016 1 PROTOCOL This evaluation was conducted on February 10, 2016 at Springs Memorial

More information

A highly sensitive single-enzyme homocysteine assay

A highly sensitive single-enzyme homocysteine assay A highly sensitive single-enzyme homocysteine assay Yuying Tan 1 & Robert M Hoffman 1,2 1 A/C Diagnostics, LLC and AntiCancer, Inc., 7917 Ostrow Street, San Diego, California 92111, USA. 2 Department of

More information

Vitamin B 12, homocysteine and carotid plaque in the era of folic acid fortification of enriched cereal grain products

Vitamin B 12, homocysteine and carotid plaque in the era of folic acid fortification of enriched cereal grain products Research Recherche Vitamin B 12, homocysteine and carotid plaque in the era of folic acid fortification of enriched cereal grain products Julie Robertson, Francesco Iemolo, Sally P. Stabler, Robert H.

More information

(a) y = 1.0x + 0.0; r = ; N = 60 (b) y = 1.0x + 0.0; r = ; N = Lot 1, Li-heparin whole blood, HbA1c (%)

(a) y = 1.0x + 0.0; r = ; N = 60 (b) y = 1.0x + 0.0; r = ; N = Lot 1, Li-heparin whole blood, HbA1c (%) cobas b system - performance evaluation Study report from a multicenter evaluation of the new cobas b system for the measurement of HbAc and lipid panel Introduction The new cobas b system provides a point-of-care

More information

LDL (Human) ELISA Kit

LDL (Human) ELISA Kit LDL (Human) ELISA Kit Cat. No.:DEIA3864 Pkg.Size:96T Intended use This immunoassay kit allows for the specific measurement of human low density lipoprotein, LDL concentrations in cell culture supernates,

More information

1. PROTOCOL. Comparison Study Summary. Tuality Healthcare 324 SE 9 th Ave. Suite E Hillsboro, OR July 30, 2014

1. PROTOCOL. Comparison Study Summary. Tuality Healthcare 324 SE 9 th Ave. Suite E Hillsboro, OR July 30, 2014 Comparison Study Summary Tuality Healthcare 324 SE 9 th Ave. Suite E Hillsboro, OR 97123 July 30, 2014 1. PROTOCOL This study was conducted on July 24 th, 2014 at Tuality Healthcare, Hillsboro, OR. The

More information

Key words: Risk factor. Hyperhomocysteinemia. Ischemic heart disease. Smoking.

Key words: Risk factor. Hyperhomocysteinemia. Ischemic heart disease. Smoking. j c p s p September 2004 Volume 14 Number 09 September 2004 Home JCPSP Home Sep JCPSP Contents Email JCPSP HYPERHOMOCYSTEINEMIA AS A RISK FACTOR FOR ISCHEMIC HEART DISEASE Muhammad Aamir, Abdus Sattar,*

More information

HbA1c (Human) ELISA Kit

HbA1c (Human) ELISA Kit HbA1c (Human) ELISA Kit Cat. No.:DEIA3509 Pkg.Size:96T Intended use GHbA1c (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human GHbA1c. General Description vhemoglobin,

More information

Rat cholesterol ELISA Kit

Rat cholesterol ELISA Kit Rat cholesterol ELISA Kit Catalog No. CSB-E11706r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat Cholesterol concentrations in serum, plasma and other biological fluids.

More information

Insulin Resistance Is Not Related to Plasma Homocysteine Concentration in Healthy Premenopausal Women

Insulin Resistance Is Not Related to Plasma Homocysteine Concentration in Healthy Premenopausal Women Physiol. Res. 55: 285-29, 26 Insulin Resistance Is Not Related to Plasma Homocysteine Concentration in Healthy Premenopausal Women F. TANRIKULU-KILIÇ, S. BEKPINAR, Y. ÜNLÜÇERÇI, Y. ORHAN 1 Department of

More information

Prospective blinded study of the relationship between plasma homocysteine and progression of symptomatic peripheral arterial disease

Prospective blinded study of the relationship between plasma homocysteine and progression of symptomatic peripheral arterial disease Prospective blinded study of the relationship between plasma homocysteine and progression of symptomatic peripheral arterial disease Lloyd M. Taylor, Jr, MD, Gregory L. Moneta, MD, Gary J. Sexton, PhD,

More information

APOB (Human) ELISA Kit

APOB (Human) ELISA Kit APOB (Human) ELISA Kit Catalog Number KA4330 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...

More information

Human LDL ELISA Kit. Innovative Research, Inc.

Human LDL ELISA Kit. Innovative Research, Inc. Human LDL ELISA Kit Catalog No: IRKTAH2582 Lot No: SAMPLE INTRODUCTION Human low-density lipoprotein (LDL) transports cholesterol from the liver to tissues where it is incorporated into cell membranes.

More information

Abraxis Progesterone (bovine) ELISA Kit

Abraxis Progesterone (bovine) ELISA Kit Abraxis Progesterone (bovine) ELISA Kit Enzyme immunoassay for the quantitative determination of progesterone in bovine milk/serum/plasma samples PN5081M 96 Tests For Research Use Only. Not for use in

More information

Serum total homocysteine concentration before and after renal transplantation

Serum total homocysteine concentration before and after renal transplantation Kidney International, Vol. 54 (1998), pp. 1380 1384 Serum total homocysteine concentration before and after renal transplantation MARGRET ARNADOTTIR, BJÖRN HULTBERG, JAN WAHLBERG, BENGT FELLSTRÖM, and

More information

Total Thyroxine ELISA (T4)

Total Thyroxine ELISA (T4) Design Verification Total Thyroxine ELISA (T4) Contents 1 Assay Principle... 2 2 Imprecision... 2 Within-run Imprecision... 2 Between run Imprecision... 2 3 Comparison of Methods, Accuracy... 2 4 Linearity...

More information

Urea Nitrogen (BUN) detection Kit

Urea Nitrogen (BUN) detection Kit K-ASSAY KAMIYA BIOMEDICAL COMPANY KAMIYA BIOMEDICAL COMPANY Urea Nitrogen (BUN) detection Kit For the quantitative determination of urea nitrogen in saliva and TCM Cat. No. KT-747 For Research Use Only.

More information

Lab Guide 2019 Metabolic Section Lab Guide

Lab Guide 2019 Metabolic Section Lab Guide Lab Guide 2019 Metabolic Section Lab Guide Quantitative Amino acids Plasma Plasma. Container/Tube: Preferred EDTA, Place immediately in ice. Acceptable: lithium heparin, sodium heparin. Patient preparation:

More information

Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit

Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit Catalog No. E0228r (96 tests) Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

More information

Cotinine (Mouse/Rat) ELISA Kit

Cotinine (Mouse/Rat) ELISA Kit Cotinine (Mouse/Rat) ELISA Kit Catalog Number KA2264 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

HOMOCYSTEINE. A Strong Risk Factor for Cardiovascular Disease INNOVATIONS IN CLINICAL DIAGNOSTICS

HOMOCYSTEINE. A Strong Risk Factor for Cardiovascular Disease INNOVATIONS IN CLINICAL DIAGNOSTICS HOMOCYSTEINE A Strong Risk Factor for Cardiovascular Disease INNOVATIONS IN CLINICAL DIAGNOSTICS About Diazyme Diazyme Laboratories, Inc., an affiliate of General Atomics, is located in Poway, California.

More information

USING THE ACCESS AMH ASSAY IN YOUR LABORATORY

USING THE ACCESS AMH ASSAY IN YOUR LABORATORY INFORMATION BULLETIN USING THE ACCESS AMH ASSAY IN YOUR LABORATORY ///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////

More information

The Adiponectin Turbidimetric Immunoassay Reagent Kit

The Adiponectin Turbidimetric Immunoassay Reagent Kit The Adiponectin Turbidimetric Immunoassay Reagent Kit Catalogue number: 51010 For the quantitative determination of Adiponectin in human serum and plasma This package insert must be read in its entirety

More information

Independent Risk Factors of Cardiovascular Disease Achieving Healthy Homocysteine Levels

Independent Risk Factors of Cardiovascular Disease Achieving Healthy Homocysteine Levels Independent Risk Factors of Cardiovascular Disease Achieving Healthy Homocysteine Levels AUTHORS: JON LEGERE, BRADLEY RALPH, DR. RON LEGERE 15344 N 83RD WAY, SCOTTSDALE, AZ 85260 TEL: 800.528.3144 EMAIL:

More information

Low-dose folic acid lowers plasma homocysteine levels in women of child-bearing age

Low-dose folic acid lowers plasma homocysteine levels in women of child-bearing age Q J Med 2002; 95:733 740 Low-dose folic acid lowers plasma homocysteine levels in women of child-bearing age S. DALY 1,J.L.MILLS 2,A.M.MOLLOY 3,M.CONLEY 2,J.MCPARTLIN 3, Y.J. LEE 2, P.B. YOUNG 5,P.N.KIRKE

More information

Relationship of Total Homocysteine, Cholesterol, Triglyceride in the Serum and Diastolic Blood Pressure of Patients with Myocardial Infarction

Relationship of Total Homocysteine, Cholesterol, Triglyceride in the Serum and Diastolic Blood Pressure of Patients with Myocardial Infarction Relationship of Total Homocysteine, Cholesterol, Triglyceride in the Serum and Diastolic Blood Pressure of Patients with Myocardial Infarction Durdi Qujeq *1, Laia Hossini 1 and M. Taghi Salehi Omran 2

More information

Papers in Press. First published August 25, 2005 as doi: /clinchem

Papers in Press. First published August 25, 2005 as doi: /clinchem Papers in Press. First published August 25, 2005 as doi:10.1373/clinchem.2005.053835 Clinical Chemistry 51:11 000 000 (2005) Automation and Analytical Techniques Automated Assay for the Determination of

More information

Human HIV (1+2) antigen&antibody ELISA Kit

Human HIV (1+2) antigen&antibody ELISA Kit Human HIV (1+2) antigen&antibody ELISA Kit Catalog Number. CSB-E18042h For the qualitative determination of human HIV (1+2) antibody and P24 antigen concentrations in serum, plasma. This package insert

More information

Homocysteine ELISA. Product information Information about other products is available at: Userś Manual

Homocysteine ELISA. Product information Information about other products is available at:  Userś Manual Product information Information about other products is available at: www.demeditec.com Userś Manual Homocysteine ELISA Enzyme immunoassay for the quantitative determination of total L-homocysteine in

More information

Triiodothyronine (T3) ELISA

Triiodothyronine (T3) ELISA For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Triiodothyronine (T3) ELISA Kit is intended for the detection of total T3 in human serum or plasma. For research

More information

Determinants and Vitamin Responsiveness of Intermediate Hyperhomocysteinemia ( 40 mol/liter)

Determinants and Vitamin Responsiveness of Intermediate Hyperhomocysteinemia ( 40 mol/liter) Determinants and Vitamin Responsiveness of Intermediate Hyperhomocysteinemia ( 40 mol/liter) The Hordaland Homocysteine Study Anne Berit Guttormsen,* Per Magne Ueland,* Ingerid Nesthus, Ottar Nygård, Jörn

More information

Apolipoprotein A1 (Apo A1) ELISA

Apolipoprotein A1 (Apo A1) ELISA Package Insert Apolipoprotein A1 (Apo A1) ELISA 1 x 96 Wells For Research Use Only (RUO). Not for use in clinical, diagnostic or therapeutic procedures. v. 1.0 Eagle Biosciences, Inc. 20A Northwest Blvd.,

More information

Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit

Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit Catalog No: E0442h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Insulin (Porcine/Canine) ELISA

Insulin (Porcine/Canine) ELISA Insulin (Porcine/Canine) ELISA For the quantitative measurement of insulin in Porcine/Canine serum and plasma (EDTA) For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSPO-E01

More information

Mouse C-Peptide ELISA Kit

Mouse C-Peptide ELISA Kit Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and

More information

DetectX. Urinary Creatinine Detection Kit. Catalog Number K002-H1. Sample Types Validated: Human, Monkey, Dog, Rat and Mouse Urine

DetectX. Urinary Creatinine Detection Kit. Catalog Number K002-H1. Sample Types Validated: Human, Monkey, Dog, Rat and Mouse Urine World s Only One Component Assay Simple & Easy to Use DetectX Urinary Creatinine Detection Kit Catalog Number K002-H1 Sample Types Validated: Human, Monkey, Dog, Rat and Mouse Urine Please read this insert

More information

P H Whincup, H Refsum, I J Perry, R Morris, M Walker, L Lennon, A Thomson, P M Ueland, S B J Ebrahim

P H Whincup, H Refsum, I J Perry, R Morris, M Walker, L Lennon, A Thomson, P M Ueland, S B J Ebrahim 448 Cardiovascular Research Unit, Department of Primary Care and Population Sciences, Royal Free Hospital School of Medicine, London NW3 2PF, UK P H Whincup R Morris M Walker L Lennon A Thomson S B J Ebrahim

More information

Canine Thyroid Stimulating Hormone, TSH ELISA Kit

Canine Thyroid Stimulating Hormone, TSH ELISA Kit Canine Thyroid Stimulating Hormone, TSH ELISA Kit Catalog No: E0463c 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

IDEXX Catalyst One Chemistry Analyzer for In-house Measurement of Total Thyroxine (TT 4 ) Concentration in Serum from Dogs and Cats

IDEXX Catalyst One Chemistry Analyzer for In-house Measurement of Total Thyroxine (TT 4 ) Concentration in Serum from Dogs and Cats IDEXX Catalyst One Chemistry Analyzer for In-house Measurement of Total Thyroxine (TT 4 ) Concentration in Serum from Dogs and Cats Authors: Kate Cote, Ph.D., Graham Bilbrough, MA, VetMB, CertVA, MRCVS,

More information

Method Comparison Report Semi-Annual 1/5/2018

Method Comparison Report Semi-Annual 1/5/2018 Method Comparison Report Semi-Annual 1/5/2018 Prepared for Carl Commissioner Regularatory Commission 123 Commission Drive Anytown, XX, 12345 Prepared by Dr. Mark Mainstay Clinical Laboratory Kennett Community

More information

Rat Hemoglobin A1c (HbA1c) Kit Instructions

Rat Hemoglobin A1c (HbA1c) Kit Instructions V.3 Crystal Chem Rat Hemoglobin A1c (HbA1c) Kit Instructions For the quantitative determination of hemoglobin A1c (HbA1c) in rat whole blood Catalog #80300 96 Assays For research use only. Not for use

More information

ACCESS hstni SCIENTIFIC LITERATURE

ACCESS hstni SCIENTIFIC LITERATURE ACCESS hstni SCIENTIFIC LITERATURE 2017 2018 Table of contents Performance Evaluation of Access hstni A critical evaluation of the Beckman Coulter Access hstni: Analytical performance, reference interval

More information

Evaluation Report: Eurolyser CRP test (ST0100 and ST0102) on. CUBE analyser (CA0100)

Evaluation Report: Eurolyser CRP test (ST0100 and ST0102) on. CUBE analyser (CA0100) Evaluation Report: Eurolyser CRP test (ST0100 and ST0102) on CUBE analyser (CA0100) Location Location: Eurolyser Diagnostica GmbH Operators: Simone Wieser; Franz Helminger; Michael Gruber Date: July-November

More information

RayBio Human Granzyme B ELISA Kit

RayBio Human Granzyme B ELISA Kit RayBio Human Granzyme B ELISA Kit Catalog #: ELH-GZMB User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

Manual. Transfer step. Figure 1. Workflow comparison of manual phenylalanine assay and automated GSP Neonatal Phenylalanine assay

Manual. Transfer step. Figure 1. Workflow comparison of manual phenylalanine assay and automated GSP Neonatal Phenylalanine assay TECHNICAL NOTE GSP Neonatal Phenylalanine kit 3308-0010/3308-001B GSP Neonatal Phenylalanine kit (3308-0010/3308-001B) is a fully Introduction automated enzymatic assay for the GSP system. Optimized for

More information

1,25 Dihydroxyvitamin D

1,25 Dihydroxyvitamin D First Unique Automated 1,25 ihydroxyvitamin First fully automated, extraction free immunoassay for the accurate detection of 1,25 ihydroxyvitamin FOR OUTSIE US & CANAA ONLY Clinical background of 1,25

More information

Human TSH ELISA Kit. User Manual

Human TSH ELISA Kit. User Manual Human TSH ELISA Kit User Manual Catalog number: GTX15585 GeneTex Table of Contents A. Product Description... 2 B. Kit Components... 3 C. Additional Required Materials (not included)... 3 D. Reagent Preparation...

More information

Homocysteine (Hcy) A Strong Risk Factor for Cardiovascular Disease. A guide to Homocysteine, correlation to human disease, and various testing methods

Homocysteine (Hcy) A Strong Risk Factor for Cardiovascular Disease. A guide to Homocysteine, correlation to human disease, and various testing methods Homocysteine (Hcy) A Strong Risk Factor for Cardiovascular Disease A guide to Homocysteine, correlation to human disease, and various testing methods Table of Contents 1. Introduction...3 2. What is homocysteine?...4

More information

25(OH) Vitamin D ELISA (BD-220BA), 192 Tests

25(OH) Vitamin D ELISA (BD-220BA), 192 Tests INTENDED USE The 25-hydroxy (25-OH) Vitamin D ELISA is intended for the quantitative determination of total 25-OH Vitamin D in human serum and Plasma. SUMMARY AND EXPLANATION Vitamin D is a steroid hormone

More information

Thyroid Stimulating Hormone (TSH) ELISA Catalog No. GWB , legacy id (96 Tests)

Thyroid Stimulating Hormone (TSH) ELISA Catalog No. GWB , legacy id (96 Tests) For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. TSH ELISA Kit is intended for the quantitative measurement of TSH in human serum or plasma. For research use only.

More information

ISSN: Asian Journal of Medical and Pharmaceutical Researches Asian J. Med. Pharm. Res. 4(2): , 2014

ISSN: Asian Journal of Medical and Pharmaceutical Researches Asian J. Med. Pharm. Res. 4(2): , 2014 \\\\ Received 12 Apri. 2014 Accepted 25 May. 2014 ORIGINAL ARTICLE 2014 Scienceline Publication www.science-line.com ISSN: 2322-4789 Asian Journal of Medical and Pharmaceutical Researches Asian J. Med.

More information

FreeStyle Lite A Blood Glucose Meter That Requires No Coding

FreeStyle Lite A Blood Glucose Meter That Requires No Coding Journal of Diabetes Science and Technology Volume 2, Issue 4, July 2008 Diabetes Technology Society SYMPOSIUM Shridhara, Ph.D. Abstract Background: Abbott Diabetes Care introduced the FreeStyle Lite blood

More information

REVIEW ARTICLE. Blood Levels of Homocysteine and Increased Risks of Cardiovascular Disease

REVIEW ARTICLE. Blood Levels of Homocysteine and Increased Risks of Cardiovascular Disease Blood Levels of Homocysteine and Increased Risks of Cardiovascular Disease Causal or Casual? REVIEW ARTICLE William G. Christen, ScD; Umed A. Ajani, MBBS; Robert J. Glynn, ScD; Charles H. Hennekens, MD

More information

Cortisol (Sheep) ELISA Kit

Cortisol (Sheep) ELISA Kit Cortisol (Sheep) ELISA Kit Catalog Number KA0919 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the

More information

Procine sphingomyelin ELISA Kit

Procine sphingomyelin ELISA Kit Procine sphingomyelin ELISA Kit For the quantitative in vitro determination of Procine sphingomyelin concentrations in serum - plasma - celiac fluid - tissue homogenate - body fluid FOR LABORATORY RESEARCH

More information

The NGAL Turbidimetric Immunoassay Reagent Kit

The NGAL Turbidimetric Immunoassay Reagent Kit Antibody and Immunoassay Services Li Ka Shing Faculty of Medicine, University of Hong Kong The NGAL Turbidimetric Immunoassay Reagent Kit Catalogue number: 51300 For the quantitative determination of NGAL

More information

Homocysteine (plasma, urine, dried blood spots)

Homocysteine (plasma, urine, dried blood spots) Homocysteine (plasma, urine, dried blood spots) 1 Name and description of analyte 1.1 Name of analyte Homocysteine 1.2 Alternative names None 1.3 NLMC code To follow 1.4. Function(s) of analyte Homocysteine

More information

Biomarkers of inflammation for population research: Stability of C-reactive protein and alpha 1 -acid glycoprotein in dried blood spots.

Biomarkers of inflammation for population research: Stability of C-reactive protein and alpha 1 -acid glycoprotein in dried blood spots. Biomarkers of inflammation for population research: Stability of C-reactive protein and alpha -acid glycoprotein in dried blood spots. Running title: CRP and AGP in dried blood spots Authors and institutions:

More information

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004 Coulter HIV p24 1. PRINCIPLE The Human Immunodeficiency Virus Type 1 (HIV-1) is recognized as the etiologic agent of acquired immunodeficiency syndrome (AIDS). The virus is transmitted by sexual contact,

More information

Unpredictable intra-individual variations in serum homocysteine levels on folic acid supplementation

Unpredictable intra-individual variations in serum homocysteine levels on folic acid supplementation European Journal of Clinical Nutrition (1997) 51, 188±192 ß 1997 Stockton Press. All rights reserved 0954±3007/97 $12.00 in serum homocysteine levels on folic acid supplementation CR Santhosh-Kumar 1,

More information

Instructions for Use. Tg Antibody ELISA

Instructions for Use. Tg Antibody ELISA Instructions for Use Tg Antibody ELISA Enzyme Immunoassay for the Quantitative Determination of Autoantibodies to Thyroglobulin (Tg) in Serum and Plasma I V D REF EA618/96 12 x 8 2 8 C DLD Gesellschaft

More information

Sally P. Stabler * and Robert H. Allen. Metabolism. Clinical Chemistry 50: (2004) Endocrinology and

Sally P. Stabler * and Robert H. Allen. Metabolism. Clinical Chemistry 50: (2004) Endocrinology and Clinical Chemistry 50:2 365 372 (2004) Endocrinology and Metabolism Quantification of Serum and Urinary S-Adenosylmethionine and S-Adenosylhomocysteine by Stable-Isotope- Dilution Liquid Chromatography

More information

BNP Fragment EIA (Cat.No. BI-20852W) For the Determination of BNP Fragment in Human Samples

BNP Fragment EIA (Cat.No. BI-20852W) For the Determination of BNP Fragment in Human Samples BNP Fragment EIA (Cat.No. BI-20852W) For the Determination of BNP Fragment in Human Samples ASSAY CHARACTERISTICS Method Competitive Enzyme Immunoassay, HRP/TMB. Microtiter plates are coated with a polyclonal

More information

25 OH Vitamin D Rapid Test

25 OH Vitamin D Rapid Test INSTRUCTION FOR USE REF:GDB 7120-25T 25 OH Vitamin D Rapid Test A Rapid Sandwich Immunochromatographic Test for Quantitative Detection of total 25-OH Vitamin D in human finger-prick blood For In Vitro

More information

Human Obestatin ELISA

Human Obestatin ELISA K-ASSAY Human Obestatin ELISA For the quantitative determination of obestatin in human serum and plasma Cat. No. KT-495 For Research Use Only. 1 Rev. 081309 K-ASSAY PRODUCT INFORMATION Human Obestatin

More information

Hyperhomocysteinemia is associated with increased risk

Hyperhomocysteinemia is associated with increased risk Supplementation of Atherogenic Diet With B Vitamins Does Not Prevent Atherosclerosis or Vascular Dysfunction in Monkeys Steven R. Lentz, MD, PhD; Donald J. Piegors, MBA; M. René Malinow, MD; Donald D.

More information

Multiple studies are available concerning the use of

Multiple studies are available concerning the use of SPECIAL ARTICLE Evaluation of the Biosite Ò Quantitative Whole Blood D-dimer Assay and Comparison With the biomérieux VIDAS Ò D-dimer Exclusion Test Validation and Utility For Use in the Central Laboratory

More information

CoQ10(Coenzyme Q10) ELISA Kit

CoQ10(Coenzyme Q10) ELISA Kit CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:

More information

GSI Canine IL-5 ELISA Kit-2 Plates DataSheet

GSI Canine IL-5 ELISA Kit-2 Plates DataSheet Interleukin5 (IL5) is a secreted glycoprotein that belongs to the α-helical group of cytokines (1, 2, 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). IL-5

More information

Association of B vitamins status and homocysteine levels in elderly Taiwanese

Association of B vitamins status and homocysteine levels in elderly Taiwanese Asia Pac J Clin Nutr 2005; 14 (3):250-255 250 Original Article Association of B vitamins status and homocysteine levels in elderly Taiwanese Kuan-Ju Chen MS, 1,2 Wen-Harn Pan PhD, 1 Feili-Lo Yang PhD,

More information

THE INFLUENCE OF TOTAL PHYSICAL INACTIVITY ON PLASMA HOMOCYSTEINE LEVELS. RISK FACTOR FOR DEVELOPMENT OF CARDIOVASCULAR DISEASE

THE INFLUENCE OF TOTAL PHYSICAL INACTIVITY ON PLASMA HOMOCYSTEINE LEVELS. RISK FACTOR FOR DEVELOPMENT OF CARDIOVASCULAR DISEASE THE INFLUENCE OF TOTAL PHYSICAL INACTIVITY ON PLASMA HOMOCYSTEINE LEVELS. RISK FACTOR FOR DEVELOPMENT OF CARDIOVASCULAR DISEASE Mihaela Jurdana*, Nadja Plazar*, and Rado Pišot** * College of Health Care

More information

Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit

Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit Rabbit Glycated hemoglobin A1c (GHbA1c) ELISA Kit Catalog No. CSB-E07011Rb (96 tests) This immunoassay kit allows for the in vitro quantitative determination of rabbit GHbA1c concentrations in cell culture

More information

Diagnostic reagent for quantitative in vitro determination of Urea / Blood Urea Nitrogen in serum, plasma and urine by colorimetry.

Diagnostic reagent for quantitative in vitro determination of Urea / Blood Urea Nitrogen in serum, plasma and urine by colorimetry. 2013/07/30 A93A01315AUS A11A01641 60 ml 15 ml Pentra C400 Diagnostic reagent for quantitative in vitro determination of Urea / Blood Urea Nitrogen in serum, plasma and urine by colorimetry. QUAL-QA-TEMP-0846

More information

Insulin ELISA. For the quantitative determination of insulin in serum and plasma.

Insulin ELISA. For the quantitative determination of insulin in serum and plasma. Insulin ELISA For the quantitative determination of insulin in serum and plasma. For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United

More information

Bovine Insulin ELISA

Bovine Insulin ELISA Bovine Insulin ELISA For quantitative determination of insulin in bovine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSBO-E01 Size: 96 wells Version:

More information

ab Sphingomyelin Assay Kit

ab Sphingomyelin Assay Kit ab133118 - Sphingomyelin Assay Kit Instructions for Use For the specific, sensitive and convenient method of quantifying Sphingomyelin in plasma or serum. This product is for research use only and is not

More information

Pilot studies with reference specimens

Pilot studies with reference specimens Pilot studies with reference specimens HUPO: Plasma Proteome Project Workshop July 16-17, 17, 2003 Daniel W. Chan, Ph.D.,DABCC Professor of Pathology, Oncology, Radiology & Urology Director of Clinical

More information

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures.

TNF-alpha ELISA. For Research Use Only. Not For Use In Diagnostic Procedures. TNF-alpha ELISA For the quantitative determination of TNF-alpha in serum, plasma, buffered solution or cell culture medium. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:

More information

Evaluation of the Precision and Accuracy of the INRatio 2 Prothrombin Time (PT) Monitoring System

Evaluation of the Precision and Accuracy of the INRatio 2 Prothrombin Time (PT) Monitoring System Evaluation of the Precision and Accuracy of the INRatio 2 Prothrombin Time (PT) Monitoring System Grace DeSantis, PhD Lisa Croner, PhD Michealle Havenhill, BA Shari Kipp, BSc (Honours) MBA Inverness Medical,

More information

Active-B12 (Holo-TC) ELISA KIT

Active-B12 (Holo-TC) ELISA KIT Active-B12 (Holo-TC) ELISA KIT Cat. No.:DEIA6312 Pkg.Size:96T Intended use The Active-B12 (Holotranscobalamin) assay is an enzyme-immunoassay (ELISA) for the quantitative determination of holotranscobalamin

More information

OxisResearch A Division of OXIS Health Products, Inc.

OxisResearch A Division of OXIS Health Products, Inc. OxisResearch A Division of OXIS Health Products, Inc. BIOXYTECH GSH/GSSG-412 TM Colorimetric Determination of Reduced and Oxidized Glutathione For Research Use Only. Not For Use In Diagnostic Procedures.

More information

Human Hemoglobin Colorimetric Detection Kit

Human Hemoglobin Colorimetric Detection Kit Human Hemoglobin Colorimetric Detection Kit CATALOG NO: IRAAKT2522 LOT NO: SAMPLE INTENDED USE The Hemoglobin detection kit is designed to quantitatively measure all forms of hemoglobin present in blood

More information

Gentian Canine CRP Immunoassay Application Note for Abbott Architect * c4000

Gentian Canine CRP Immunoassay Application Note for Abbott Architect * c4000 v2-jan214 Gentian Canine CRP Immunoassay Application Note for Abbott Architect * c4 Intended Use The canine CRP immunoassay on Abbott's Architect c4 is an in vitro diagnostic test for quantitative determination

More information

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum

More information

Porcine/Canine Insulin ELISA

Porcine/Canine Insulin ELISA Porcine/Canine Insulin ELISA For the quantitative determination of insulin in porcine or canine serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research

More information

Instructions for use. Histamine Release

Instructions for use. Histamine Release Instructions for use Histamine Release Supplementary kit for the determination of the release of histamine from heparinized whole blood (this kit has to be used in combination with the Histamine ELISA,

More information

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #:

Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3 Required

More information

ab Creatinine Assay Kit (Colorimetric)

ab Creatinine Assay Kit (Colorimetric) ab204537 Creatinine Assay Kit (Colorimetric) Instructions for Use For the quantitative determination of Creatinine in urine samples. This product is for research use only and is not intended for diagnostic

More information

Mouse Ultrasensitive Insulin ELISA

Mouse Ultrasensitive Insulin ELISA Mouse Ultrasensitive Insulin ELISA For the quantitative determination of insulin in mouse serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research Use

More information

Liquid Liquid Extraction of Gamma Hydroxybutyric Acid (GHB) in Blood and Urine for GC-MS analysis

Liquid Liquid Extraction of Gamma Hydroxybutyric Acid (GHB) in Blood and Urine for GC-MS analysis Liquid Liquid Extraction of Gamma Hydroxybutyric Acid (GHB) in Blood and Urine for GC-MS analysis 1.0 Purpose - This procedure specifies the required elements for the extraction of GHB from blood, urine

More information

European Journal of Clinical Investigation (2003) 33, VU University Medical Center, Amsterdam, the Netherlands

European Journal of Clinical Investigation (2003) 33, VU University Medical Center, Amsterdam, the Netherlands European Journal of Clinical Investigation (2003) 33, 17 25 Blackwell Science, Ltd S-adenosylhomocysteine and the ratio of S-adenosylmethionine to S-adenosylhomocysteine are not related to folate, cobalamin

More information