Beta Amyloid Peptides

Transcription

1 Beta Amyloid Peptides The Most Comprehensive Collection for Alzheimer s Disease Academic Services Pharmaceutical Services

2 Beta Amyloid Peptides The Most Comprehensive Collection for Alzheimer s Disease Proprierary FlexPeptide TM technology with combined platform, microwave and ligation system used for amyloid peptide synthesis. igher quality with less expensive price. Superior Purity All products purity. Rigorous QC Test Products must pass extensive quality control, such as PLC, MS, etc. Wide Selection ver 59 amyloid products available. We also provide customized peptides on special request. Quick rdering System All products are in stock and promptly shipped following purchase. State-of-The-Art Facilities GMP What are Beta Amyloid Peptides Beta amyloid is a protein fragment that forms amyloid plaques in the brain of Alzheimer's disease (AD) patients. Beta amyloid peptide consists of 43 amino acids. It is created via the enzymatic digestion of amyloid precursor protein (APP) by beta secretase and gamma secretase. It then forms insoluble fibrous aggregates, causing amyloidosis and neurodegenerative diseases such as AD. Due to beta amyloid's pathology, it has become one of the most investigated molecules in Alzheimer's disease research. Why choose our Beta Amyloid Peptides GenScript's FlexPeptide TM system and click peptide platform allows us to synthesize a comprehensive array of human and rat beta amyloid peptide fragments. Testing these fragments can help elucidate the dynamic biological interactions of beta amyloid peptide and facilitate a better understanding of Alzheimer s disease at the molecular level. At GenScript, we are dedicated to providing the most current and highest quality reagents in the global effort to treat and find a cure for Alzheimer s disease. With our philosophy of always striving to be on the forefront of new technology and its application in treating disease, you can be confident you have a committed partner to further your research and future innovations. Advanced Technologies 1. FlexPeptide TM Technology includes: a. Combined platform GenScript has several LPPS and SPPS methods that ensure high peptide synthesis success rates. b. Microwave technology The smart and efficient way to optimize peptide synthesis yield c. Ligation technology Long peptides up to 200 aa can be synthesized. 1

3 Case study : β-amyloid (1-40) Sequence: Asp - Ala - Glu - Phe - Arg - is - Asp Leu - Met - Val - Gly - Gly - Val - Val Length: 40 aa Purity: 95.55% PLC results mv 300 Chromatogram min 1 Det.A Ch1 / 220nm MS results Positive Intensity Mass Spectrum [M+4] [M+a+4] [M+3] m/z 2

4 Case study III: β-amyloid (25-35) Sequence: Gly - Ser - Asn - Lys - Gly - Ala - Ile - Ile - Gly - Leu - Met Length: 11 aa Purity: 95.18% PLC results mv 400 Chromatogram min Det.A Ch1 / 220nm MS results Positive Intensity [M+2]2+ Mass Spectrum [M+] m/z 3

5 2. Click Peptide Platform The preparation of hydrophobic peptides like beta-amyloid can be a time consuming process. Even after peptides are successfully prepared, proper dissolving of peptides is critical. At GenScript, our featured click peptide platform is specially designed to handle the low water-solubility and aggregative nature of hydrophobic peptides. (Xaa) m 2 (Xaa) n Xaa: amino acid - intramolecular acyl migration p 7.4 ne way and quick process (Xaa) m (Xaa) (ydrophilic, chick peptide) - acyl lsopeptide (ydrophobic, native peptide) A click peptide based on the -acyl isopeptide method Case Study : 26--acyl β-amyloid (1-42) click peptide was successfully synthesized. The β-ester bond was quickly and quantitatively converted to a native Gly25-Ser26 amide bond via a p-dependent - intramolecular acyl migration reaction (t1/2 =1 min, p 7.4, 37 ) at a hydroxyamino acid residue. amely, upon this p-triggered conversion (p-click), the non-aggregative and water-soluble precursor (click peptide) can produce the monomer with a random-coil structure under physiological conditions (p 7.4, 37 ). The structure information is as follows. Aβ Aβ to- acyl migration p 7.4 ne way and quick process Aβ1-24 Aβ acyl isoaβ 1-42 (click peptide) amyloid β peptide (Aβ) 1-42 β-amyloid (1-42) click peptide Results β-amyloid precursor(click peptide) has a water solubility of 1/ml, while it is only 0.14 mg/ml for the native peptide. The aggregative property of the peptide is significantly reduced. The -acyl moiety was stable under acidic p. 4

6 The following are PLC reports of purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor) and purified 26--acyl β-amyloid (1-42) click peptide (precursor). mv Chromatogram Det.A Ch1 / 220nm Detector A Ch1 220nm Peak# Total Ret. Time Area Peak Table eight Area % PLC report of purified β-amyloid (1-42) native peptide converted from β-amyloid (1-42) click peptide (precursor) min mv Chromatogram Det.A Ch1 / 220nm min Detector A Ch1 220nm Peak# Total Ret. Time Area eight Area %

7 rdering List: Category Cata o. Purity ame Size RP β-amyloid (1-28) RP β-amyloid (1-40) RP04-1 β-amyloid (1-40) RP08-1 β-amyloid (25-35) RP08-5 β-amyloid (25-35) RP β-amyloid (1-42), rat RP13-1 β-amyloid (1-42), rat RP β-amyloid (1-40), rat RP16-1 β-amyloid (1-40), rat RP β-amyloid (1-42), human RP17-1 β-amyloid (1-42), human RP β-amyloid (42-1) RP34-1 β-amyloid (42-1) RP20101 β-amyloid (1-33) RP20102 β-amyloid (11-22) RP20103 β-amyloid (1-16) RP20104 β-amyloid (12-28) RP20105 β-amyloid (18-28) RP20106 β-amyloid (1-11) RP β-amyloid (22-35) RP β-amyloid (22-35) RP20138 β-amyloid (1-37) RP20139 β-amyloid (1-36) RP20140 β-amyloid (1-34) RP20141 β-amyloid (1-15) RP20142 β-amyloid (26-40) RP20143 β-amyloid (30-34) RP20144 β-amyloid (33-40) RP20145 β-amyloid (35-42) RP20146 β-amyloid (8-38) RP20148 β-amyloid (17-24) 6

8 Category Cat o Purity ame Size RP20150 β-amyloid (22-40) RP20152 β-amyloid (16-23) RP20153 β-amyloid (16-24) RP20154 β-amyloid (16-26) RP20160 β-amyloid (1-9) RP20161 β-amyloid (1-17) RP20162 β-amyloid (1-20) RP20163 β-amyloid (10-35) RP20165 β-amyloid A4 Protein Precursor ( ), APP (C31) RP20166 β-amyloid (1-40) Binding Peptide RP20168 Cys Containing β-amyloid (1-40) Binding Peptide RP20169 Parallel topology β-amyloid modified peptide RP20170 β-amyloid (13-27) RP β-amyloid (15-21) RP β-amyloid (15-21) RP β-amyloid (16-20) RP β-amyloid (16-20) RP β-amyloid (4-10) RP β-amyloid (4-10) RP20174 β-amyloid (10-20) RP20175 β-amyloid (12-20) RP β-amyloid (32-35) RP β-amyloid (32-35) RP β-amyloid (29-40) RP β-amyloid (29-40) RP β-amyloid (31-35) RP β-amyloid (31-35) 7

9 Category Cat o Purity ame Size RP β-amyloid (35-25) RP β-amyloid (35-25) RP β-amyloid (35-25) RP20186 β-amyloid (37-43) RP20192 β-amyloid (7-22) RP20195 β-amyloid Protein Precursor ( ) RP β-amyloid Protein Precursor 770 ( ) RP β-amyloid Protein Precursor 770 ( ) Mca-(Asn 670,Leu 671 )-Amyloid RP20207 β/a4 Protein Precursor770 ( )-Lys(Dnp) Mca-(Asn 670,Leu 671 )-Amyloid RP20208 β/a4 Protein Precursor770 ( )-Lys(Dnp), amide RP20265 Beta-Amyloid (10-25) RP20266 Amyloid Precursor Protein (APP) ( ) RP20278 Beta-Amyloid (36-42) RP20352 Beta-amyloid peptide (1-8) RP20367 Beta-amyloid(33-42) RP20405 Beta-Amyloid (11-17) RP20483 Cys-Beta-Amyloid (25-35) RP20505 Beta-Amyloid(1-14),mouse,rat RP β-amyloid (1-42), human RP β-amyloid (1-42), human RP β-amyloid (1-40) RP β-amyloid (1-40) GenScript offer customized bulk synthesis, please send your request via product@genscript.com For current price, visit For related amyloid antibodies, visit 8

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