Roc On with These Dependable LC Columns

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2 Roc LC s Conventional column built to be the cornerstone for your lab; pressure rated for any 400 bar system. Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you demand. Exceptional value for routine analyses and large-volume workflows high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most methods. Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods. Newly designed minimal packaging for compact storage download serial #-specific certificates of analysis at Get more of what you want, and less of what you don t, with Roc LC columns from Restek. Order today at Roc Family of LC Phases C8 C8 Phenyl-Hexyl Cyano Silica C N OH CH 3 Si CH 3 O CH 3 Si CH 3 O CH 3 Si CH 3 O CH 3 Si O CH 3 Si O O O C8 Cyano Phenyl-Hexyl USP Phase Code L L7 L L0 L3 Stationary Phase Category C8, octadecylsilane C8, octylsilane phenyl cyano bare silica Ligand Type monomeric C8 monomeric C8 phenyl-hexyl cyanopropyl n/a Particle Size 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical 3 μm or 5 μm, spherical Pore Size 00 Å 00 Å 00 Å 00 Å 00 Å Surface Area 300 m /g 300 m /g 300 m /g 300 m /g 300 m /g Carbon Load 0% % 5% 8% n/a End-Cap yes yes yes yes n/a ph Range.5 to to to to to 8.0 Maximum Temperature 80 C 80 C 80 C 80 C 80 C

3 Roc On with These Dependable LC s Figure : Over,000 injections and Roc columns will still offer reliable performance, regardless of which dimension your method calls for % Efficiency vs # of Injections Roc 5 µm 375 bar x 4.6 mm 50 x 4.6 mm 50 x 4.6 mm 00 x 3.0 mm 50 x 3.0 mm 50 x 3.0 mm Figure : Add a Roc LC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency. Peaks tr (min) (mg/ml). Uracil Toluene Naphthalene Biphenyl With guard column : Roc C8 (cat.# ); Dimensions: 50 mm x 4.6 mm ID; ; Pore Size: 00 Å; Guard : Roc C8 0 mm, 4.0 mm ID, 5 µm (cat.# ); ; : Custom mix; Diluent: 50:50 Acetonitrile:water; Inj. Vol.: µl; : A: Water, B: Acetonitrile; Without guard column Gradient (%B): 0.00 min (70%), 8.00 min (70%); Flow:.0 ml/min; : 54 nm; :. 3 k =.7 Asymmetry =.07 Plates/m = 97,5 4 k =.7 Asymmetry =.097 Plates/m = 9,053 LC_GN0565 Figure 3: From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again. Reversed Phase Peaks tr (min). p-tolunitrile Butylbenzoic acid ,4-Dimethylanisole Mefenamic acid p-xylene Lot Peaks tr (min). Benzene 3.5. Nitrobenzene 4. 3.,3,5-Trinitrobenzene ,4-Dinitrotoluene 7.4 Normal Phase Lot 5 Lot Lot Lot 3 LC_GN056 : Roc C8 (cat.# ); Dimensions: 50 mm x 4.6 mm ID; ; Pore Size: 00 Å; Temp.: 35 C; Inj. Vol.: 5 µl; : 0.05% Formic acid in water:0.05% formic acid in acetonitrile (40:60); Flow:.0 ml/min; : 0 nm; Cell Temp: 35 C; :. Lot LC_GN0563 : Roc Cyano (cat.# 95363E); Dimensions: 00 mm x 3.0 mm ID; Particle Size: 3 µm; Pore Size: 00 Å; Temp.: 40 C; : Inj. Vol.: µl; : Water:acetonitrile (80:0); Flow: 0.6 ml/min; : 54 nm; Cell Temp.: 40 C; :. Find the entire Roc column line and order now at 3

4 Roc LC s from Restek Perform Better Than or Equal To s from Other Vendors Table I: Roc LC columns offer equivalent or better peak shape, retention, and resolution compared to FPP columns from other manufacturers under identical conditions (average values, n = 5). s Analyte tr Area Height USP Tailing Asymmetry (0%) k' Plate Number Resolution Ethylparaben 3.5,407,609 8, ,738 Roc 5.40,869,684 34, ,64.5 Ethylparaben 3.060,44,6 5, ,73 Vendor A 4.889,860,659 3, , Ethylparaben.873,46,068 39, ,706 Vendor B 4.307,867,049 87, , Ethylparaben.763,43,099 35, ,643 Vendor C 4.580,868,74 70, ,700.9 Ethylparaben 3.57,939,87 357, ,478 Vendor D 5.86,77,785 8, ,954. Vendor E Ethylparaben 3.873,498,446 44, , ,78,453 74, , Chromatographic conditions for Table I and Figure 4 (USP Monograph 38, ) All columns are C8 stationary phase (5 µm, 50 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (:); Flow rate: ml/min; Injection volume: 5 µl; : with UV detector, 80 nm; : 00 µg/ml ethinyl estradiol and 50 µg/ml ethylparaben in water:acetonitrile (:). 4

5 Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Roc LC. Ethylparaben estradiol Roc C8 (cat.# ) Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX058 Vendor A. Ethylparaben estradiol Vendor C C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX05 5

6 (Continued from page 5) Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Vendor B. Ethylparaben estradiol Vendor B C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 0 Å Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX050 Vendor C. Ethylparaben estradiol Vendor A C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 80 Å Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX

7 (Continued from page 6) Figure 4: Count on Roc LC columns from Restek for outstanding chromatographic performance. Vendor D. Ethylparaben estradiol Vendor D C8 Dimensions: 50 mm x 4.6 mm ID Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX05 Vendor E. Ethylparaben estradiol Vendor E C8 Dimensions: 50 mm x 4.6 mm ID Pore Size: 00 Å Inj. Vol.: 5 µl Flow (ml/min) %A , 4 nm LC_EX

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