YMC-Actus. Columns for Semi-preparative HPLC. Selectivity Durability Loadability
|
|
- Jane Ramsey
- 6 years ago
- Views:
Transcription
1 YMC-Actus Columns for Semi-preparative HPLC Selectivity Durability Loadability
2 Fast semi-preparative chromatography Semi-preparative chromatography is the link between analytical HPLC and preparative LC. Even though the chromatographic systems used for semi-preparative LC are not as large as preparative LC systems, the objectives remain the same: Purification and isolation of maximum sample quantity Savings in time and costs. With YMC-Actus, time is on your side! 2 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:
3 Stability Rapid pressure changes under high-speed gradient conditions may lead to column degradation and loss of column performance. With YMC-Actus, a new hardware and packing technology has been applied to these semipreparative columns to provide a uniform packing density which results in a longer lifetime than conventional semi-preparative columns. In order to determine the quality of the packing, the column performance has been evaluated every 100 runs with a sequential high-throughput gradient. YMC-Actus columns offer outstanding efficiency without compromising resolution. Furthermore, YMC-Actus columns provide reliable results, even after exposure to severe, rapid gradient conditions and multiple injections. Increase column lifetime = Decrease of cost Conventional Semi-preparative Column Sequential gradient test Colum: 50 x 20 mm ID, 5 µm or 50 x 19 mm ID, 5 µm Eluent: Gradient: A) water B) methanol 5% B (0-0.5 min), 5-95% B ( min), 95% B ( min), 5% B ( min) Flow rate: 50 ml/min Temp.: ambient Column performance test Column: 50 x 20 mm ID, 5 µm or 50 x 19 mm ID, 5 µm Eluent: methanol / water (70/30) Flow rate: 10 ml/min Sample: Naphthalene Application data by courtesy of YMC Co., Ltd. 3
4 YMC-Actus ION EXCHANGE MEDIA Axial Compression Technology for Ultimate Separation YMC-Actus series columns are semi-preparative HPLC columns that have excellent column stability and efficiency as a result of applying axial compression technology. YMC-Actus series columns show high stability under high flow rate or steep gradient conditions which are desirable for milligram scale preparative HPLC of various compounds. 10% increase in bed density Compressing Flow Test conditions (high-speed and high-pressure) Column: 5 µm, 50 x 20 mm ID Eluent: A) water B) methanol Gradient: 5%-95% B Flow rate: 50 ml/min Pressure: ~ 17 MPa Long Lasting Columns! after 700 runs initial Conventional Column Packing without compression Flow Void and crack Degradation of performance after 300 runs initial Uniformly high density packing is necessary for highly efficient and stable HPLC columns. DAC (Dynamic Axial Compression) columns are widely used for preparative separation in pilot or production scale. This allows uniformly high density packing and prevents formation of voids. YMC-Actus series columns have been developed by applying this Axial Compression Technology to semi-prep column production. The column bed is compressed appropriately when attaching the inlet end assembly of the newly designed YMC-Actus hardware. It provides increased bed density (10% higher than conventional columns) and bed uniformity. YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website: 4
5 YMC-Actus ION EXCHANGE MEDIA Excellent stability and efficiency under fast gradient conditions at high flow rate Glycyrrhizic acid in herb medicine Analysis Purification YMC-Pack ODS-AQ, 50 x 4.6 mm ID, 5 µm 3 ml/min, 10 µl injection YMC-Actus ODS-AQ, 50 x 20 mm ID, 5 µm 60 ml/min, 500 µl injection After purification 99.5% Glycyrrhizic acid Glycyrrhizic acid Available for high-throughput purification: Injection in DMSO 1. Ethenzamide 2. Clemastine fumarate 3. Ibuprofen Column: Eluent: Gradient: Flow rate: Temperature: Detection: Injection: YMC-Actus Pro C18 RS (5 µm, 8 nm), 50 x 20 mm ID A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 10-90% B (0-3 min), 90% B (3-5 min), 10% B (5-10 min) 20 ml/min ambient UV at 270 nm 100 µl (0.2-2 mg/ml in DMSO) As shown in overlay chromatograms, YMCActus columns provide outstanding stability and reproducibility in the separation of pharmaceuticals dissolved in 100% DMSO, even after 1,000 injections under the test conditions. This makes YMC-Actus columns ideal for high-throughput purification in drug discovery. Application data by courtesy of YMC Co., Ltd. 5
6 Excellent separation of compounds with similar structure Capsaicinoids in red pepper Analysis 50 x 4.6 mm ID, 5 µm 2.0 ml/min, 20 µl injection Phenomenex Luna C18, 5 µm, 10 nm Waters XBridge C18, 5 µm YMC-Pack Pro C18 RS Purification YMC-Actus Pro C18 RS, 50 x 20 mm ID 5 µm Nordihydrocapsaicin Capsaicin Dihydrocapsaicin 40 ml/min, 400 µl injection Purity >99.5% Eluent: A) methanol / water / TFA (50/50/0.1) B) methanol / TFA (100/0.1) 0-30% B (0-5 min, 30% B (5-10 min) Temperature: 25 C for 50 x 4.6 mm ID ambient for 50 x 20 mm ID Detection: UV at 280 Sample: methanol extract of a commercial red pepper (1 g / 3 ml) Outstanding separation of highly polar compounds Crude synthetic 30mer oligonucleotide Analysis Hydrosphere C ml/min, 5 µl injection Purification 19 ml/min, 100 µl injection 50 x 4.6 mm ID, 5 µm 50 x 20 mm ID, 5 µm Impurities 30mer YMC-Actus Hydrosphere C18 Recovery 56% purity>99% 6 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:
7 YMC-Actus Preparative Column Selection Finder Optimisation of preparative chromatography! The main task for a preparative chromatographer is to find the suitable system. In order to simplify the considerations YMC developed a Preparative Column Selection Guide. R&D Scale-up Kit: Analytical and semipreparative columns packed with a stationary phase from the same lot. The Preparative Column Selection Guide will help to select:1.the most suitable column ID for the required sample loading 2.the appropriate particle size for optimum efficiency 3.the required column length for the necessary resolution R&D Kits To minimise the problems that the process of scale-up may introduce, YMC offers YMC R&D kits, which consist of one analytical column and one YMC-Actus column packed with exactly the same packing material from the same production lot. Therefore no further method development is needed, just a simple linear scale-up calculation. Application data by courtesy of YMC Co., Ltd. 7
8 Linear Scale-Up In most cases it is beneficial to develop a semi-preparative method on an analytical scale column. The analytical separation carried out on a 150 x 4.6 mm ID column has to be scaled up to 150 x 20 mm ID Therefore the chromatographic parameters such as flow rate and column load have to be adjusted according to the following equation: Linear Scale-Up SF = P 2 ID, prep P 2 ID, anal. = m prep m anal. SF = r 2 ID, prep / r 2 ID, analytical SF = 20 2 ID, prep / ID, analytical SF = g 4.6 mm ID 20 mm ID 37.8 g Inner diameter loadability / compound Guideline for Sample Load according to column ID Column ID (mm) Scale-Up factor Loadability (mg) YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:
9 Scale-Up YMC linear scale-up is achieved in 4 steps: 1. Analytical scale method development. Determine the optimum separation conditions by using analytical columns packed with different stationary phases and various conditions. 2. Study the preparative scale. Select the particle size of the packing material and the inner diameter of column appropriate for the sample volume. 3. Optimise the separation conditions and perform loadability studies using analytical columns with inner diameter of 4.6 mm or 6.0 mm packed with the packing material selected for the preparative separation (scout column). If the particle size of the packing material is the same as in the Step 1, this process can be omitted. If the preparative column is more than 100 mm ID, it is advisable to insert another step with a scout column of 20 mm ID in order to accurately predict loadability and calculate the running costs. 4. Proceed with the preparative separation with scale-up of chromatographic parameters such as flow rate/ column ID/ sample load as necessary. From all the given steps above the most demanding step will be the scale-up of the chromatographic parameters in order to meet the preparative demands. There are a number of scalable parameters: flow rate, column ID, sample load, tubing ID, sample injection concentration, volume of sample loop, consumption of solvent, dead volume,fraction mass, size of the detector cell. How to adjust the scalable factors? In order to simplify your scale-up the three most important scale-up factors are summarised. Scalable factor SF Internal Diameter Linear Scale-Up Column length Time Column length and ID Volume SF = r 2 ID, prep / r 2 ID, anal. SF = l ID, prep / l ID, anal. SF = (r 2 ID, prep / r 2 ID, anal.) / (l ID, prep / l ID, anal. ) Impact Flow rate Eluent composition Retention time Cycle time Plate number Amount of adsorbent Application data by courtesy of YMC Co., Ltd. 9
10 Ordering Information High durability semi-preparative columns Packing material Particle size Pore size Column size [µm] [nm] length x ID [mm] Product Code 50 x 20 TA12S WX 100 x 20 TA12S WX 150 x 20 TA12S WX Triart C18 S x 20 TA12S WX 50 x 30 TA12S WX 75 x 30 TA12S05-L530WX 100 x 30 TA12S WX 50 x 20 AS12S WX 100 x 20 AS12S WX 150 x 20 AS12S WX Pro C18 S x 20 AS12S WX 50 x 30 AS12S WX 75 x 30 AS12S05-L530WX 100 x 30 AS12S WX 50 x 20 HS12S WX 100 x 20 HS12S WX 150 x 20 HS12S WX Hydrosphere C18 S x 20 HS12S WX 50 x 30 HS12S WX 75 x 30 HS12S05-L530WX 100 x 30 HS12S WX 50 x 20 RS08S WX 100 x 20 RS08S WX 150 x 20 RS12S WX Pro C18 RS S x 20 RS12S WX 50 x 30 RS08S WX 75 x 30 RS08S05-L530WX 100 x 30 RS08S WX 50 x 20 OS12S WX 100 x 20 OS12S WX 150 x 20 OS12S WX Pro C8 S x 20 OS12S WX 50 x 30 OS12S WX 75 x 30 OS12S05-L530WX 100 x 30 OS12S WX 50 x 20 AA12S WX 100 x 20 AA12S WX ODS-A S x 20 AA12S WX 50 x 30 AA12S WX 75 x 30 AA12S05-L530WX 100 x 30 AA12S WX 50 x 20 AQ12S WX 100 x 20 AQ12S WX ODS-AQ S x 20 AQ12S WX 50 x 30 AQ12S WX 75 x 30 AQ12S05-L530WX 100 x 30 AQ12S WX YMCbasic S x 20 BA99S WX Other phases are available on request. Columns with 50 mm ID are available on request. 10 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:
11 Ordering Information Guard cartridges Packing material Particle size [µm] Pore size [nm] Triart C18 S-5 12 Pro C18 S-5 12 Hydrosphere C18 S-5 12 Pro C18 RS S-5 8 Pro C8 S-5 12 ODS-A S-5 12 ODS-AQ S-5 12 Column size length x ID [mm] Product Code 10 x 20 TA12S GC 20 x 30 TA12S GC 10 x 20 AS12S GC 20 x 30 AS12S GC 10 x 20 HS12S GC 20 x 30 HS12S GC 10 x 20 RS08S GC 20 x 30 RS08S GC 10 x 20 OS12S GC 20 x 30 OS12S GC 10 x 20 AA12S GC 20 x 30 AA12S GC 10 x 20 AQ12S GC 20 x 30 AQ12S GC YMCbasic S x 20 BA99S GC Guard Cartridge Column Holder: Product Code XPCHSPW2 Application data by courtesy of YMC Co., Ltd. 11
12 Your local distributor: Co., Ltd. YMC Karasuma-Gojo Bld. 284 Daigo-cho, Karasuma Nisiiru Gojo-dori Shimogyo-ku, Kyoto Japan TEL. +81(0) , FAX +81(0) India Ltd. CX - 07, 3rd Floor, Lobe - 1, Tower - A, The Corenthum, Plot No- A-41, Sector - 62, Noida (UP) India. TEL. +91(0) , FAX +91(0) Europe GmbH Schöttmannshof 19 D Dinslaken Germany TEL. +49(0)2064/427-0, FAX +49(0)2064/ Korea Co., Ltd. #208, Owners Tower, 16-5, Sunae-dong, Bundang-gu, Seongnami-si, Gyeonggi-do, Korea TEL , FAX America, Inc. 941 Marcon Boulevard Suite 301 Allentown, PA18109 USA TEL , FAX Co., Ltd. Shanghai Rep. Office Far East International Plaza A2404 No. 319 Xianxia Road, Shanghai P.R. China TEL: , FAX:
Purification of Insulin with YMC-Triart Prep C8. Increased Output at Reduced Costs
Purification of Insulin with YMC-Triart Prep C8 Increased Output at Reduced Costs 2 Introduction YMC bulk material along with phases from Daiso and Kromasil took part in a study by a major peptide manufacturer.
More informationYMC HILIC Columns for Polar Analytes
YMC HILIC Columns for Polar Analytes Polar Analytes Advantages In general, reliable applications in HPLC require a minimum retention factor (k ) of 2. In the widely used reversed phase methods polar compounds
More informationMeteoric Core. Core-Shell Columns for UHPLC & HPLC. Easy Method Optimization! Excellent Chromatography with Fast Separation!
Meteoric Core Easy Method Optimization! Excellent Chromatography with Fast Separation! Core Porous shell layer Meteoric Core Particle Image Structure Schöttmannshof 19 46539 Dinslaken Phone +49 (0)2064
More informationYMC Chiral columns. Polysaccharides NEA(R)(S) CD BR
YMC Chiral columns Polysaccharides NEA(R)(S) CD BR HPLC Columns for Optical Isomer Separation Chirality has become vitally important in the production of pharmaceuticals, agrochemicals, food and related
More informationYMC-Triart C18. Multilayer hybrid material for HPLC ph and temperature stability. Innovative micro-reactor technology
YMC-Triart C18 Versatile hybrid silica based HPLC column The Selectivity Company Multilayer hybrid material for HPLC ph and temperature stability Innovative micro-reactor technology www.ymc.de 2 YMC-Triart
More informationYMC-Triart C18. Multi-layer hybrid material for HPLC ph and temperature stability. Innovative micro-reactor technology
YMC-Triart C18 Versatile hybrid silica based HPLC Column The Selectivity Company Multi-layer hybrid material for HPLC ph and temperature stability Innovative micro-reactor technology www.ymc.de 2 YMC-Triart
More informationSize Exclusion Chromatography YMC-Pack Diol
Size Exclusion Chromatography YMC-Pack Diol www.ymc.de What is special about YMC SEC-Columns? Method development Scalability Reproducibility Cost-effective YMC-Column for SEC: YMC-Pack Diol Analysis of
More informationYMC CHIRAL columns. Polysaccharides NEA(R)(S) CD BR
YMC CHIRAL columns Polysaccharides NEA(R)(S) CD BR HPLC Columns for Optical Isomer Separation Chirality has become vitally important in the production of pharmaceuticals, agrochemicals, food and related
More informationHPLC. Varian Dynamax Preparative HPLC Columns
HPLC Varian Dynamax Preparative HPLC Columns Preparative HPLC Simple, successful and economical Linear scale-up move from analytical separation to preparative purification quickly and simply. Dynamic Axial
More informationOverview on YMC Column Hardware and Phases
Overview on and Phases Content UHPLC Column Hardware PT and CC...page 03 Analytical Column Hardware PTH and WT...page 04 Analytical Column Hardware QT and QC...page 05 Analytical Column Hardware GC...page
More informationYMC-Triart C18. Multilayer hybrid material for HPLC ph and temperature stability. Innovative micro-reactor technology
YMC-Triart C18 Versatile hybrid silica based HPLC column The Selectivity Company Multilayer hybrid material for HPLC ph and temperature stability Innovative micro-reactor technology www.ymc.de 2 YMC-Triart
More informationOverview on YMC Column Hardware and Phases
Overview on and Phases Contents UHPLC Column Hardware PT and CC...page 02 Analytical Column Hardware PTH and WT...page 03 Analytical Column Hardware QT and QC...page 04 Analytical Column Hardware GC...page
More informationAeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE
Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance
More informationMass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System
Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent
More informationChoosing Preparative Columns
Choosing Preparative Relationship between Column I.D., Sample Loading Volume and Flow Rate In preparative operations, column internal diameter sizes from 6.0 to 100 mm are widely used. When the analytical
More information3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis.
We are pleased to present the 11th YMC General Catalog. Since its formation in 1980, YMC Co., Ltd. has been working in the rapidly changing field of chemistry. Over this time, the rate of discovery of
More informationAnalytical and Preparative SFC Columns
Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...
More informationYMC*Gel HG-series. High grade silica phases for preparative HPLC. The Selectivity Company
YMC*Gel HG-series High grade silica phases for preparative HPLC The Selectivity Company www.ymc.de Company profile YMC is a leading specialist supplier of high performance products for liquid chromatography
More informationPreparative Chromatography Meets UHPLC
Preparative Chromatography Meets UHPLC The Power of Kinetex Core-Shell Technology with the Performance of Piston Packed Prep Columns Ultra-High Efficiencies Ultra-Long Lifetimes NO Added Pressure Two Award
More informationA New Era in SFC Column Technology
B TM A ew Era in SFC Column Technology Combining state-of-the-art media manufacturing with the industry leading column technology, Viridis Supercritical Fluid Chromatography (SFC) columns bring a new level
More informationColumns for HPLC. Columns for HPLC
Basic principles of preparative HPLC Basically, preparative HPLC follows the same rules as analytical scale chromatography. However, there are important differences in the aims of the two techniques. In
More informationAnalytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 120 PFP
Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 1 PFP Application Note Clinical Research Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd Abstract
More informationEffective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate
Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More informationSepax Technologies, Inc.
Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid
More informationRebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica
Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica This
More informationExceed your Purification Goals. Guaranteed. Phenomenex l WEB:
Exceed your Purification Goals Guaranteed The Axia Prep Column Advantage Extended column lifetime Reproducibility: column-to-column and batch-to-batch igher efficiency Improved peak shape Increased loadability
More informationComparison of a UPLC Method across Multiple UHPLC Systems
Comparison of a UPLC Method across Multiple UHPLC Systems Tanya Jenkins Waters Corporation, Milford, MA, U.S. INTRODUCTION In 2004, Waters introduced the ACQUITY UPLC System. Since this launch, many liquid
More informationLC Columns - Exceed the limit. A premium inert range of LC columns delivering optimal peak shape. ProteCol -P PEEK lined
ProteCol LC Columns - Exceed the limit A premium inert range of LC columns delivering optimal peak shape. ProteCol -G Glass lined ProteCol -P PEEK lined B C A D E F A Column end cap B PEEK frit housing
More informationSUMMARY, CONCLUSION & RECOMMENDATIONS
196 Chapter-5 SUMMARY, CONCLUSION & RECOMMENDATIONS 197 CHAPTER 5 5.1 Summary, Conclusion and Recommendations Summary and Conclusion are drawn based on the work carried out by the author on development
More informationNormal Phase HPLC Column COSMOSIL SL-II
Normal Phase HPLC Column COSMOSIL SL-II High purity silica gel (>99.99%) with special treatment Suitable for preparative separation Ultra-pure silica gel of more than 99.99% purity is used for the COSMOSIL
More informationApplication Note. Preparative Isolation of Piperine from Black Pepper Extracts. Summary. Introduction. Fig. 1
Application Note Preparative Isolation of Piperine from Black Pepper Extracts Category Food analysis Matrix Pepper Extract Method analytical HPLC, preparative HPLC Keywords Pepper, quality control, purity
More informationSelectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants
Selectivity Comparison of Agilent Poroshell 1 Phases in the Separation of Butter Antioxidants Application Note Food Testing & Agriculture Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd. Abstract
More informationRoc On with These Dependable LC Columns
Roc LC s Conventional column built to be the cornerstone for your lab; pressure rated for any 400 bar system. Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you
More informationIon Exchange Chromatography COSMOGEL IEX Series Columns
Ion Echange Chromatography COSMOGEL IEX Series Columns Available in 3 different ion-echange modes Anion-echange type, Cation-echange type, Amphoteric ion-echange type Available for 3 different application
More informationPrep Columns. and Bulk Media. and Bulk Media
Prep Columns and Bulk Media Axia Columns...346-355 Process Chromatography...356-36 Bulk Media...356-36 Columns, Scout and Preparative...359-36 Sepra Bulk Sorbents... 36 Prep Columns and Bulk Media 345-36
More informationCore-Shell Technology for Proteins and Peptides
Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein
More informationAPPLICATIONS TN µm Core-Shell Particle. (continued on page 2) Page 1 of 8. For additional technical notes, visit
Kinetex 5 µm Core-Shell Columns Deliver Significant Improvements in Chromatographic Efficiency and Resolution versus Traditional Fully Porous 5 µm Columns Philip J. Koerner and Jeff Layne Phenomenex, Inc.,
More information4 Columns for HTS and LC/MS Analysis
YMC GENERAL CATALG Columns for HTS and LC/MS Analysis Semi-micro columns for LC/MS YMC-UltraHT series YMC CombiChrom 9 9 97 98,99 93 第 章.indd 93 11.1.8 1:51:39 AM Semi-micro columns for LC/MS Wide selection
More informationDetermination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography
Determination of Tetracyclines in Chicken by Solid-Phase Extraction and High-Performance Liquid Chromatography Application ote Food Safety Authors Chen-Hao Zhai and Yun Zou Agilent Technologies Co. Ltd.
More informationImpurity Profiling of Carbamazepine by HPLC/UV
Application Note: 52049 Impurity Profiling of Carbamazepine by HPLC/UV Terry Zhang, Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela Hypersil GOLD Carbamazepine Drug Analysis
More informationInertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior
InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups,
More informationEurospher II the logical choice. Physical properties of Eurospher II silica gel: Silica gel: ultra pure, > % Metal content: < 10 ppm
HPLC SMB smometry Euroline Eurospher II Eurospher II the logical choice After more than years on the market, our Eurospher stationary phase has established a reputation for being a first-class packing
More informationSmall Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application
Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Pharmaceutical Author Cliff Woodward Agilent Technologies, Inc. 285 Centerville
More informationMicroLC. MicroLC. CapillaryLC NanoLC
MicroLC MicroLC CapillaryLC NanoLC Introduction Miniaturisation of liquid chromatography in combination with mass spectrometry has several advantages including improvements in sensitivity, especially at
More information176 YMC Chiral Columns
176 YMC Chiral Columns YMC Chiral Columns 177 Chiral Columns Content YMC Chiral NEA(R)(S)... 178-181 YMC Chiral CD BR... 182-185 Ordering Information... 186 HPLC Columns for Optical Isomer Separation Introduction
More informationACE C18-AR Use the Power!
combines the retention and separation characteristics of a C18 phase with the extra selectivity of an integral phenyl functional group. The combination provides extra resolving power for a wide variety
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationApplication Note. Separation of clindamycin phosphate and process impurities. Summary. Introduction. Category Pharmaceutical analysis Matrix
Application Note Separation of clindamycin phosphate and process impurities Category Pharmaceutical analysis Matrix Drugs Method UHPLC Keywords Antibiotics, process impurities, quality control Analytes
More informationInertSustainBio C18. Rapid Separations of Proteins and Peptides
InertSustainBio C18 Rapid Separations of Proteins and Peptides High Recoveries of Proteins and Peptides The 200A pore size silica creates the opportunity to separate compounds having a molecular weight
More informationUltimate Core Performance to Maximize Your Investment
Thermo Scientific Accucore HPL olumns Phase Overview Ultimate ore Performance to Maximize Your Investment Phase Overview Founded on state-of-the-art ore Enhanced Technology and utilizing vast experience
More informationPurification - Flash Chromatography
SuperFlash Compound Purification Columns Optimal Design Cost effective, high performance disposable flash column specifically designed for flash chromatography. Excellent Separations Designed to deliver
More informationFigure 1. Core-Shell Particle Technology
Purification of Chlorogenic cid from Green Coffee using Kinetex Core-Shell Technology in xia Preparative Formats Zeshan qeel Technical Magician Zeeshan loves to collect watches and the ack to the Future
More informationA New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis
A New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis X. Liu, C. Pohl, Dionex Corporation, Sunnyvale, CA, USA ABSTRACT Although reversed-phase (RP) silica columns (e.g., C18 and
More informationProduct Guide for LudgerSep TM ur2 UHPLC Column for DMB Sialic Acid Analysis
Product Guide for LudgerSep TM ur2 UHPLC Column for DMB Sialic Acid Analysis Product # LS-UR2-2.1x100 Ludger Document # LS-uR2-DMB-Guide-v2.1 Ludger Ltd Culham Science Centre Oxford OX14 3EB United Kingdom
More informationProduct Guide for LudgerSep TM R1 HPLC Column for DMB labelled Sialic Acid Analysis
Product Guide for LudgerSep TM R1 HPLC Column for DMB labelled Sialic Acid Analysis Product # LS-R1-4.6x150 Ludger Document # LS-R1-DMB-Guide-v5.1 Ludger Ltd Culham Science Centre Oxford OX14 3EB United
More informationYMC Phases for Biochromatography IEX SEC RP NP/HILIC
YMC Phases for Biochromatography IEX SEC RP NP/HILIC YMC Phases for Biochromatography Historically, small molecules have played the major role in diagnosis and therapy. However with the recent developments
More informationMaximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system
Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author
More informationASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC
ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry
More informationACE HILIC + + UHPLC and HPLC Columns. For Reproducible Polar Analyte Separations. Three HILIC Column Chemistries Available
ACE HILIC UHPLC and HPLC s For Reproducible Polar Analyte Separations Three HILIC Chemistries Available ACE HILIC s What is HILIC? Hydrophilic Interaction Liquid Chromatography (HILIC) was first described
More informationReversed-Phase Columns (Other than ODS)
7 Reversed-Phase Columns (ther than DS) Types and characteristics of reversed-phase columns -- 9, 9 YMC-Pack Pro C8, C--------------------------- 96 YMC-Pack C 8 --------------------------------------
More informationAPPLICATIONS TN Overview of Kinetex 2.6 µm Core-Shell Technology
Determination of Impurities and Related Substances for Acetylsalicylic Acid (Ph. Eur. Monograph 9): Increased Sensitivity and Resolution, Faster Analysis, and Reduced Solvent Usage Using Kinetex 2.6 µm
More information- intermediate polarity between normal phase silica and other alkyl bonded reversed phases - operates in either normal phase or reversed phase mode
Normal Phases 125 YMC-Pack TMS (C1) - intermediate polarity between normal phase silica and other alkyl bonded reversed phases L13 USP - operates in either normal phase or reversed phase mode YMC-Pack
More informationUltra Columns. also available. ordering note
: 3μm or 5μm particles; 100Å pore size Our broadest selection of stationary phases, including unique phases. High density bondings, for maximum retention. High-purity, Type B silica gives excellent peak
More informationHICHROM. Chromatography Columns and Supplies NEW PRODUCTS. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies NEW PRODUCTS Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: sales@hichrom.co.uk
More informationAm I getting the very best value from my UHPLC analyses?
Am I getting the very best value from my UHPLC analyses? Stephen Luke LC Columns Product Manager 1 Primary reasons for UHPLC use Very fast Very high resolution Column 2.1 x 50 mm Column 2.1 x 150 mm Run
More informationUnequalled durability against water elution. % tr
Unequalled durability against water elution 2 Revolutionary aqueous durability for aminopropyl phase 1 3 4 1. fructose (2.5mg/mL) 3. sucrose (2.5mg/mL) 500 hr 2. glucose 400 hr Aqueous to non-aqueous Normal
More informationConverting a CHP Method for Insulin to Agilent Poroshell 120 Columns
Converting a CHP Method for Insulin to Agilent Poroshell Columns Application Note Biopharm Author Rongjie Fu Agilent Technologies (Shanghai) Co., Ltd. 4 Ying Lun Road Shanghai 3 China Abstract A regulatory
More informationPAPRIKA EXTRACT SYNONYMS DEFINITION DESCRIPTION FUNCTIONAL USES CHARACTERISTICS
PAPRIKA EXTRACT Prepared at the 77 th JECFA, published in FAO JECFA Monographs 14 (2013), superseding tentative specifications prepared at the 69 th JECFA (2008). An ADI of 0-1.5 mg/kg bw was allocated
More informationKromasil The way to peak performance in liquid chromatography
Kromasil The way to peak performance in liquid chromatography Kromasil product catalogue Kromasil is offered in a wide assortment of columns and in bulk amounts from parts of kilograms to metric tons.
More informationDikma Technologies Inc.
Dikma HPLC / UHPLC Columns Selection Guide HPLC Column Selection Chart Sample MW Sample Solubility Separation Mode Dikma Recommended Column Page Fat Soluble Normal Phase Platisil NH 2 Platisil CN Platisil
More informationAchiral SFC: Development of an Orthogonal SFC Method for Mometasone Furoate Impurity Analysis
Achiral SFC: Development of an Orthogonal SFC Method for Mometasone Furoate Impurity Analysis Zhenyu Wang Merck Research Laboratories NJCG 2013 Outline SFC chronicle The journey of a half century Re-birth
More informationLC-MS/MS Method for the Determination of Tenofovir from Plasma
LC-MS/MS Method for the Determination of Tenofovir from Plasma Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 687 Key Words SPE, SOLA CX, Hypersil GOLD, tenofovir Abstract
More informationThe high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns.
The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. Fast High peak capacity Low back pressure PROTEIN PEPTIDE UHPLC COLUMNS Put these new, high-performance
More informationChromatogram Search Tool Search by compound name, synonym, CAS # or keyword Pinnacle II Columns
: 3μm or 5μm particles; 110Å pore size Silica at, for total control of quality and reproducibility. Excellent replacement for the original Hypersil material. Physical and chromatographic properties similar
More informationSPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine
SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA
More informationAgilent Anion-Exchange Media for Proteins - Loading vs Resolution - Effect of Flow Rate and Example Protein Separations
Agilent Anion-Exchange Media for Proteins - Loading vs Resolution - Effect of Flow Rate and Example Protein Separations Technical Overview Introduction PL-SAX is a hydrophilic strong anion-exchange chromatographic
More informationSeparation of Macrocyclic Lactones (Avermectins) on FLARE C18 MM & FLARE C18+ Columns
Separation of Macrocyclic Lactones (Avermectins) on FLARE C8 MM & FLARE C8+ Columns Introduction Diamond Analytics Technical Note: T05- Avermectins are a series of 6-membered macrocyclic lactone derivatives
More informationUNISON UK - Amino For Aqueous Elutions and Exceptional Separation Balance
UNISON UK - Amino For Aqueous Elutions and Exceptional Separation Balance Revolutionary aqueous durability Aqueous to non-aqueous High speed normal phase separation 3μm particle size Superior resolution
More informationLC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column - Shodex TM HILICpak TM VG-50 2D
LC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column - Shodex TM HILICpak TM VG-50 2D Introduction Components of pharmaceutical products and food products often include high
More informationAPPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology.
Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology Jeff Layne, Phenomenex, Inc. Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 951 USA With virtually
More informationPEEK SS Columns & Guard Columns
PEEK SS Columns & Guard Columns ~ Stainless Steel Analytical Columns ~ PEEK Columns ID 2.1, 4.0, 4.6 & 7.5 mm ~ Jour-Guard Cartridges Stainless Steel or PEEK ~ Stainless Steel End Fittings 1/4 OD Columns
More informationAPPLICATIONS TN A Comparison of Various Kinetex C18 Phases USP: L1
APPLICATIONS A Comparison of Various Kinetex C18 Phases Zeshan Aqeel and Jeff Layne Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 91 USA Introduction When performing reversed phase LC separations, there
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationSeQuant ZIC -HILIC For all who expect more...
SeQuant ZIC -ILIC For all who expect more... The better choice for PLC and LC/MS of all types of polar and hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany Poor
More information(U)HPLC columns for polar analytes
(U)HPLC columns for polar analytes The Selectivity Company YMC-Triart C8 for 00% aqueous conditions Universal 2 columns in : C8 phase with AQ compatibility Flexible UHPLC HPLC ph = - 2 Temp. up to 70 C
More informationSeQuant ZIC -HILIC For all who expect more...
SeQuant ZIC -ILIC For all who expect more... The better choice for PLC and LC/MS of all types of polar and hydrophilic compounds EMD Millipore is a division of Merck KGaA, Darmstadt, Germany Poor retention
More informationACE Excel UHPLC Columns
Excel UHPLC Columns info@ace-hplc.com Advanced Chromatography Technologies Ltd www.ace-hplc.com Australia & New Zealand contact: infor@winlab.com.au or call + 0 Outline Introduction Key Product Points
More informationCYCLOSERINI CAPSULAE - CYCLOSERINE CAPSULES (AUGUST 2015)
August 2015 Document for comment 1 2 3 4 5 CYCLOSERINI CAPSULAE - CYCLOSERINE CAPSULES DRAFT PROPOSAL FOR THE INTERNATIONAL PHARMACOPOEIA (AUGUST 2015) DRAFT FOR COMMENT 6 Should you have any comments
More informationF. Al-Rimawi* Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, East Jerusalem. Abstract
JJC Jordan Journal of Chemistry Vol. 4 No.4, 2009, pp. 357-365 Development and Validation of Analytical Method for Fluconazole and Fluconazole Related Compounds (A, B, and C) in Capsule Formulations by
More informationUSP purity analysis of pravastatin sodium using the Agilent 1120 Compact LC
USP purity analysis of pravastatin sodium using the Agilent 1120 Compact LC Application Note Manufacturing QA/QC Authors Syed S. Lateef, Siji Joseph Agilent Technologies Bangalore, India 500 400 Pravastatin
More informationUltra Columns HPLC COLUMNS
Acetaminophen, Narcotic Analgesics.............519 Aldehydes, Ketones...........................500 Beclomethasone.............................523 Corticosteroids..............................529 Explosives..................................501
More informationIJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet
More informationPhenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases
Phenyl-Hexyl UHPLC Columns lternate, complementary selectivity to A C8 and C8 bonded phases articularly recommended for P compounds containing aromatic groups xcellent bonded phase stability for E durable,
More informationApplication Note. Authors. Abstract. Food
Determination of Hormones in Shrimp by Agilent 129 Infinity LC with Agilent Poroshell 12 LC Column and Agilent Bond Elut QuEChERS for Sample Preparation Application Note Food Authors Rongjie Fu and Andy
More informationDetermination of enantiomeric purity of Timolol Maleate by Supercritical Fluid Chromatography
Determination of enantiomeric purity of Timolol Maleate by Supercritical Fluid Chromatography Dr. Damian Connolly, (PMBRC, WIT) & Mr. Adrian Marley (Allergan Pharmaceuticals, Westport) PMBRC 3 rd Analytical
More informationUltra High Performance Liquid Chromatograph. Nexera X2 C196-E079
Ultra High Performance Liquid Chromatograph Nexera X2 C196-E79 Maximizing the Potential of UHPLC/HPLC Analyses Intelligence Intelligent data solutions, including a new i -PDeA peak calculation method for
More informationAsian Journal of Chemistry Vol. 21, No. 1 (2009),
Asian Journal of Chemistry Vol. 21, o. 1 (2009), 176-182 imultaneous Estimation of Related Impurities of Tizanidine Hydrochloride in its Active Pharmaceutical Ingradient by Reversed-Phase Liquid Chromatography
More informationComprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids
PO-CON1576E Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids HPLC 2015 PSB-MULTI-06 Yoshiyuki WATABE, Tetsuo IIDA, Daisuke NAKAYAMA, Kanya TSUJII, Saki
More information