3/28/17. Cellular Respiration. Chapter 9: Cellular Respiration & Fermentation. Chapter 9: Cellular Respiration & Fermentation

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1 Chapter 9: Cellular Respiration & Fermentation SE C TION 1: C E LLULAR RE SP IRATION: AN OVERVIEW As we learned last chapter, energy from the sun is transformed into different forms. In this chapter you will learn how cellular respiration transforms the energy into a form that our cells are able to use. this chapter focuses on how cells use the energy stored in food molecules to make ATP Chemical Energy for Animals Cellular Respiration: is the process by which cells make energy for life processes. Cells take the carbon present in glucose & turn it into stable carbon dioxide & energy In simple terms, it is the process where our bodies break down foods and the cells convert stored chemical energy from the food into ATP, which is the energy the cells use ATP is the portable form of energy used inside of cells Cellular Respiration is basically the opposite of photosynthesis. Cellular Respiration The products of photosynthesis are the reactants of respiration. In other words, plants and animals work together! biochemical pathway Adenosine Tri-Phosphate ATP: is the main source of energy for cells. ATP is made of a nitrogen base, a sugar, and 3 phosphates Energy holds the 3 rd phosphate to the other 2. When the 3 rd phosphate is released, ATP becomes Adenosine Di- Phosphate (ADP). The energy connector is also released, and energy is available for cell processes. Chapter 9: Cellular Respiration & Fermentation SE C TION 2: TH E P ROC E SS OF C E LLULAR RESPIRATION 1

2 Cellular Respiration (ATP Production) Glycolysis is the first step in respiration. It yields a small amount of ATP. It then leads to 1 of 2 paths depending on the presence or absence of oxygen: Anaerobic Fermentation occurs if oxygen is absent. It yields no additional ATP. Aerobic Respiration occurs if there is oxygen present. It yields more ATP. The amount of oxygen present determines how much ATP can be produced Glycolysis is complicated here s what happens: Glycolysis It takes place in the cytoplasm of the cell. 1 molecule of glucose (sugar) enters. 2 molecules of pyruvic acid are made. 2 molecules of NADH are made 2 (net) molecules of ATP are made. Glycolysis Summary: the process that breaks down glucose to pyruvate, producing a small amount of ATP Chapter 9: Cellular Respiration & Fermentation SE C TION 3: FE RME NTATION Anaerobic Respiration Fermentation occurs after glycolysis if there is no oxygen (in the absence of oxygen). No ATP is produced, but NAD+ is. NAD+ is used to keep glycolysis going. Other products are lactic acid or ethyl alcohol. Summary of Fermentation: Process that recycles NAD+ in the absence of oxygen so that carbohydrates can continue to be broken down to produce ATP. Occurs to enable glycolysis to continue Lactic Acid Fermentation Lactic Acid Fermentation Pyruvic acid (from glycolysis) is converted to lactic acid. Lactic acid fermentation is used in the manufacture of yogurt and cheese. This process occurs in muscle cells when you exercise very hard. It occurs when there is not enough oxygen. The increased acidity in the cell cytosol reduces the ability of muscles to contract. This causes muscle fatigue, pain, & cramping. 2

3 Alcoholic Fermentation Some plants and unicellular organisms use alcoholic fermentation rather than lactic acid fermentation. Ethyl alcohol fermentation is used in making beer and wine. It is also used by yeast in bread-making. The ethyl alcohol is burned off, and the CO 2 bubbles make the bread rise. Chapter 9: Cellular Respiration & Fermentation SE C TION 2: TH E P ROC E SS OF C E LLULAR RESPIRATION Aerobic Respiration Aerobic Respiration Our cells usually have oxygen available. This allows for aerobic respiration. Nearly 20 times the ATP can be produced (over glycolysis)!!! Remember, we started with glycolysis which gave us 2 ATPs. Since we now have oxygen available, fermentation is skipped Remember the 2 Pyruvic acid molecules from glycolysis? The pyruvic acid enters the mitochondria (in eukaryotes) It is used to make 2 acetyl CoA, 2 NADH, & 2 CO 2 Your muscles don t hurt as bad, and you can keep going longer! (Of course, you re working at a slower rate.) 3

4 Aerobic Respiration Krebs Cycle Aerobic Respiration has 2 major stages: 1. Krebs Cycle NAD+ is changed to NADH. FAD+ is changed to FADH 2 In prokaryotes, this occurs in the cytosol. In eukaryotes, this occurs in the mitochondria. 2. Electron Transport Chain NADH and FADH 2 is used to make ATP. 1 NADH makes 3 ATP 1 FADH 2 makes 2 ATP Pyruvic Acid (from glycolysis) is converted into Acetyl CoA to start the Krebs Cycle CO 2, Hydrogen, and ATP are produced. Along with NADH and FADH 2 Krebs Cycle Krebs Cycle 1 molecule of glucose makes 2 molecules of pyruvic acid, which makes 2 Acetyl CoA Therefore, the Krebs cycle must turn twice. Only 2 ATPs are produced (the same as glycolysis). 6 NADH molecules and 2 FADH 2 molecules are made. They drive the rest of aerobic respiration. Krebs Cycle Summary: a series of chemical reactions that break down Acetyl CoA, producing ATP & electron carriers that enter an ETC how_the_krebs_cy cle_works quiz_1_.html Electron Transport Chain Electron Transport Chain The ETC is the final stage of aerobic respiration. Up to 34 (net) ATPs are produced (this is the stage of aerobic respiration that produces the most ATP) The NADH & FADH 2 produced previously during glycolysis, the conversion of pyruvic acid, & the Krebs Cycle are converted into ATP during this process Remember: 1 NADH = 3 ATP 1 FADH 2 = 2 ATP The electrons grab Hydrogen ions and throw them out of the mitochondrial matrix. The Hydrogen naturally wants to leak back in (by diffusion). This leaking helps turn ADP back into ATP. Oxygen is the final electron acceptor. 4

5 ETC Amount of Energy Produced The efficiency of aerobic respiration is roughly 66%. That may not seem like much, but your car is only about 25% efficient!!! Amount of Energy Produced Summarizing Cellular Respiration Glycolysis is extremely inefficient!!! It is only about 3.5% efficient. It is enough to provide energy for some unicellular and very small multi-cellular organisms, though. Most of the energy is contained in pyruvic acid, not the 2 ATPs produced. Reactants: glucose and oxygen Products: carbon dioxide, water, and energy C 6 H 12 O 6 + 6O 2 à 6CO 2 + 6H 2 O + energy (Glucose + Oxygen à Carbon Dioxide + Water + Energy) Cellular Respiration Organic Compounds ( Glucose ) cellular respiration - the process by which cells make ATP by breaking down glucose or other food molecules as you can see, the equations for photosynthesis and respiration have similar pieces photosynthesis produces glucose and cellular respiration uses it photosynthesis removes CO 2 from the atmosphere and respiration puts it back photosynthesis releases O 2 into the atmosphere, and cellular respiration uses it Oxygen absent Fe rme ntation Glycolysis Pyruvic acid Oxygen present Aerobic Respiration The Krebs Cycle Electron Transport Chain ATP 2 ATP & 2 NADH 5

6 What is the difference between cellular respiration & photosynthesis? They are backwards in chemical equation of each other Cellular respiration produces ATP as an end product while photosynthesis produces glucose(sugar) as an end product What do they have in common? H2O CO2 O2 Glucose Energy (but different types) There are 3 main steps in glycolysis. What are the starting & ending products for each step? In the first step, glucose is the starting product and two three-carbon sugars are the end products. In the second step the three-carbon sugars are the starting products and NADH are the ending products. In the third step, the modified three-carbon sugar from the second step is converted to pyruvate; four molecules of ATP are produced. What happens during the Krebs cycle as electrons pass down the electron transport chain? Where does this step take place? How does the ETC during the Krebs cycle affect the hydrogen ion concentration in a mitochondria? Energy is transferred into each molecule through which the electrons pass. The electrons are transported in the inner membranes of mitochondria. Hydrogen ions are pumped out of the inner chamber of the mitochondria, setting up a concentration gradient for hydrogen ions across the membrane. What is the role of ATP synthase in the Krebs cycle? Hydrogen ions diffuse through this enzyme, providing energy for ATP production. How is water formed as a by-product of the Krebs cycle? Oxygen combines with hydrogen ions and spent electrons to form water. If oxygen is not present, the electron transport chain stops and the Krebs cycle also stops. 6

7 Which is most efficient in producing ATP: glycolysis, fermentation, or the Krebs cycle? Why? Cellular Respiration The Krebs cycle(aerobic respiration) because it includes the electron transport chain, which produces up to 34 molecules of ATP, which are added to the 2 ATP molecules produced by the Krebs cycle glycolysis produces a net of 2 ATP molecules fermentation produces NO ATP molecules. The total amount of ATP produced through aerobic respiration is 38 Glycolysis Fermentation: w/o O2 after glycolysis Production of Acetyl CoA: w/o2 after glycolysis Krebs Cycle 7

8 ETC Total ATP Production 8

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