Ion Source. Mass Analyzer. Detector. intensity. mass/charge

Size: px
Start display at page:

Download "Ion Source. Mass Analyzer. Detector. intensity. mass/charge"

Transcription

1 Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector

2 Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y

3 Liquid Chromatography (LC)-MS/MS Ion Source LC Analyzer 1 Fragmentation Analyzer 2 Time Detector

4 Ion Sources Ion Source MALDI ESI Analyzer Detector

5 Electrospray Analyzer Detector

6 Matrix Assisted Laser Desorption Ionization (MALDI) Analyzer Detector V Laser Matrix alpha-cyano-4-hydroxycinnamic acid

7 Detectors Ion Source Analyzer Detector Electron Multiplier Image Current

8 Electron Multiplier Detector

9 Image Current Detector

10 Analyzers Ion Source Analyzer Time-of-Flight Magnetic Sector Quadrupole Ion Trap (3D, linear) FTICR Orbitrap Detector

11 Spectrometry (MS) dv F = ma = m = z( E + v B) dt m dv = E + v B z dt

12 Time-of-Flight Spectrometry zv L = = vt mv 2 2 Ion Source Analyzer Time-of-Flight L Detector Detector t = L 2V m z V

13 Magnetic Sector F = zv = mv R 2 2 mv 2 = zvb R = m z 2V B

14 Quadrupole Filter U V cos( ωt) y x U + V cos( ωt)

15 Ion Trap

16 Fourier transform ion cyclotron resonance F = mv = zvb R 2 m z = R v B = B ω

17 Orbitrap

18 Quadrupole Time-of-Flight Spectrometer Ion Source Analyzer 1 CAD Collision Activated Dissociation Fragmentation Analyzer 2 Detector Quadrupole Time-of-Flight Video

19 Triple Quadrupole Ion Source Analyzer 1 CAD Collision Activated Dissociation Fragmentation Analyzer 2 Quadrupole Quadrupole Quadrupole Detector m/z m/z NO m/z m/z time m/z time YES m/z time time time time m/z m/z YES m/z time time m/z is constant time

20 ESI Linear Ion Trap / Orbitrap Linear Ion Trap HCD Ion Source Analyzer 1 CAD ETD Fragmentation Detector Fragmentation Analyzer 2 Detector Orbitrap Olsen J V et al. Mol Cell Proteomics 2009;8: Video

21 Data Independent Acquisistion 1. MS 2. MS/MS 1 3. MS/MS 2 4. MS/MS 3 5. MS 6. MS/MS 1 7. MS/MS 2 8. MS/MS 3 9. MS 10. MS/MS MS/MS MS/MS MS 14. MS/MS MS/MS MS/MS MS 18. MS/MS MS/MS MS/MS MS 22. MS/MS MS/MS MS/MS 3

22 Data Dependent Acquisistion 1. MS 2. MS/MS 1 3. MS/MS 2 4. MS/MS 3 5. MS/MS 4 6. MS/MS 5 7. MS/MS 6 8. MS/MS 7 9. MS/MS MS/MS MS/MS MS 13. MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS MS/MS 10

23 Vacuum System Ion Source Vacuum System Analyzer Detector Atmosphere

24 Spectrometry Data Ion Source Analyzer Detector

25 Spectrometry Data Dimensions: Time Peptide m/z Peptide Intensity Petide fragment m/z Peptide fragment...

26 Example data MALDI-TOF m/z Peptide vs m/z m/z

27 Example data ESI-LC-MS/MS Peptide vs m/z vs time m/z Time 100 % Relative Abundance [M+2H] m/z Fragment vs m/z MS/MS

28 Slice - Scalable Data Sharing for Remote Informatics Developed by Manor Askenazi slice.ionomix.com Most mass spectrometry data is acquired in discovery mode, meaning that the data is amenable to open-ended analysis as our understanding of the target biochemistry increases. In this sense, mass spectrometry based discovery work is more akin to an astronomical survey, where the full list of object-types being imaged has not yet been fully elucidated, as opposed to e.g. micro-array work, where the list of probes spotted onto the slide is finite and well understood.

29 Example Spectrometry Data Peptide vs time For m/z which corresponds to 3+ of LGEHNIDVLEGNEQFINAAK

30 Example Spectrometry Data Fragment vs m/z For 3+ of LGEHNIDVLEGNEQFINAAK

31 Example Spectrometry Data Fragment vs m/z For 3+ of LGEHNIDVLEGNEQFINAAK

32 Example Spectrometry Data 3+ of LGEHNIDVLEGNEQFINAAK Peptide vs m/z

33 Example Spectrometry Data 3+ of LGEHNIDVLEGNEQFINAAK Peptide vs m/z

34 Example Spectrometry Data Peptide vs m/z Peptide vs time

35 Openslice.fenyolab.org

36 Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector

1. Sample Introduction to MS Systems:

1. Sample Introduction to MS Systems: MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:

More information

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique

More information

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit

More information

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods

More information

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample

More information

Biological Mass Spectrometry. April 30, 2014

Biological Mass Spectrometry. April 30, 2014 Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing

More information

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according

More information

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction

More information

Fundamentals of Soft Ionization and MS Instrumentation

Fundamentals of Soft Ionization and MS Instrumentation Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:

More information

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Content Lectures 1-3 The basics of mass measurement Ionisation techniques Mass analysers Detectors

More information

Introduction to Proteomics 1.0

Introduction to Proteomics 1.0 Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using

More information

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document

More information

SCS Mass Spectrometry Laboratory

SCS Mass Spectrometry Laboratory SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training

More information

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19, MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents

More information

Components of a Mass Spectrometer

Components of a Mass Spectrometer Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,

More information

New Instruments and Services

New Instruments and Services New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion

More information

MALDI-TOF. Introduction. Schematic and Theory of MALDI

MALDI-TOF. Introduction. Schematic and Theory of MALDI MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein

More information

New Instruments and Services

New Instruments and Services New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo

More information

REDOX PROTEOMICS. Roman Zubarev.

REDOX PROTEOMICS. Roman Zubarev. REDOX PROTEOMICS Roman Zubarev Roman.Zubarev@ki.se Physiological Chemistry I, Department for Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm What is (RedOx) Proteomics? Proteomics -

More information

Biological Mass spectrometry in Protein Chemistry

Biological Mass spectrometry in Protein Chemistry Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of

More information

Proteomics/Peptidomics

Proteomics/Peptidomics Proteomics/Peptidomics System biology tools and preclinical models for translational research in endometriosis, ESHRE Campus workshop, 4-5 September 2009 E. Waelkens Proteomics: What? Proteins Proteomics

More information

Time (min) Supplementary Figure 1: Gas decomposition products of irradiated DMC.

Time (min) Supplementary Figure 1: Gas decomposition products of irradiated DMC. 200000 C 2 CH 3 CH 3 DMC 180000 160000 140000 Intensity 120000 100000 80000 60000 40000 C 2 H 6 CH 3 CH 2 CH 3 CH 3 CCH 3 EMC DEC 20000 C 3 H 8 HCCH 3 5 10 15 20 25 Time (min) Supplementary Figure 1: Gas

More information

Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University

Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University Mass Spectrometry at the Wageningen University Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC-

More information

5/11/2015 MASS SPECTROMETRY. Mass spectrometry (MS) - overview. Mass spectrometer. Ionization and mass analyzer. Analyzer: QUADRUPOLE

5/11/2015 MASS SPECTROMETRY. Mass spectrometry (MS) - overview. Mass spectrometer. Ionization and mass analyzer. Analyzer: QUADRUPOLE UNIVERSITY OF PÉCS MEDICAL SCHOOL www.medschool.pte.hu MASS SPECTROMETRY Mass spectrometry (MS) - overview SAMPLE: charged/ionized sample(fluid biological samples, proteins, peptides, oligosaccharides,

More information

Solving practical problems. Maria Kuhtinskaja

Solving practical problems. Maria Kuhtinskaja Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements

More information

Shotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.

Shotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance. Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample

More information

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett

More information

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100

More information

PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System

PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow

More information

Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry

Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry PO-CON1347E Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry ASMS 2013 TP-708 Matthew S. F. Choo 1,3 ; Roberto Castangia 2 ; Matthew E.

More information

Comparison of mass spectrometers performances

Comparison of mass spectrometers performances Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion

More information

NIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05.

NIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05. NIH Public Access Author Manuscript Published in final edited form as: J Proteome Res. 2013 July 5; 12(7): 3071 3086. doi:10.1021/pr3011588. Evaluation and Optimization of Mass Spectrometric Settings during

More information

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010 MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited

More information

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its

More information

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies. Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in

More information

Characterization of an Unknown Compound Using the LTQ Orbitrap

Characterization of an Unknown Compound Using the LTQ Orbitrap Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown

More information

MALDI Imaging Mass Spectrometry

MALDI Imaging Mass Spectrometry MALDI Imaging Mass Spectrometry Nan Kleinholz Mass Spectrometry and Proteomics Facility The Ohio State University Mass Spectrometry and Proteomics Workshop What is MALDI Imaging? MALDI: Matrix Assisted

More information

Metabolomics: quantifying the phenotype

Metabolomics: quantifying the phenotype Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems

More information

Protein Analysis using Electrospray Ionization Mass Spectroscopy *

Protein Analysis using Electrospray Ionization Mass Spectroscopy * OpenStax-CNX module: m38341 1 Protein Analysis using Electrospray Ionization Mass Spectroscopy * Wilhelm Kienast Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons

More information

More structural information with MS n

More structural information with MS n PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with

More information

Choosing the metabolomics platform

Choosing the metabolomics platform Choosing the metabolomics platform Stephen Barnes, PhD Department of Pharmacology & Toxicology University of Alabama at Birmingham sbarnes@uab.edu Challenges Unlike DNA, RNA and proteins, the metabolome

More information

Agilent 7700x ICP-MS

Agilent 7700x ICP-MS Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler

More information

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016 LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry

More information

MS/MS to Targeted Proteomics (MRM)

MS/MS to Targeted Proteomics (MRM) MS/MS to Targeted Proteomics (MRM) How it worked on the Human Lens Proteome Jayson Falkner PhD jay@singleorganism.com Genes Show Limited Value in Predicting Diseases With only a few exceptions, what the

More information

MS/MS Scan Modes. Eötvös University, Budapest April 16, MS/MS Scan Modes. Árpád Somogyi. Product Ion Scan Select. Scan. Precursor Ion Scan Scan

MS/MS Scan Modes. Eötvös University, Budapest April 16, MS/MS Scan Modes. Árpád Somogyi. Product Ion Scan Select. Scan. Precursor Ion Scan Scan MS/MS Modes Árpád Somogyi Eötvös University, Budapest April 16, 2012 MS/MS Modes Product Ion Precursor Ion Neutral Loss Δ ed Reaction Monitoring (SRM) 1 modes in a triple quadrupole (QqQ) (one quadrupole

More information

MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation

MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN Dissertation zur Erlangung des akademischen Grades Doktor rerum naturalium (Dr. rer. nat.) vorgelegt

More information

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems

More information

Polymer Additive Analysis by EI and APCI

Polymer Additive Analysis by EI and APCI Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable

More information

Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF

Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,

More information

Amadeo R. Fernández-Alba

Amadeo R. Fernández-Alba % of compounds % of compounds % of compounds % of compounds Amadeo R. Fernández-Alba LC-Orbitrap QExactive Focus Instrumental LOQ 1% 9% 8% 7% 6% 5% 4% 3% 2% 1% %.1 mg/g.2 mg/g Tomato.5 mg/g ddms2 Target

More information

Mass spectrometry imaging. What does MS imaging offer?

Mass spectrometry imaging. What does MS imaging offer? BMG 744 Mass spectrometry imaging Stephen Barnes, PhD With sincere acknowledgments to David Stella, PhD and Kyle A. Floyd, MS, former students in the Barnes Laboratory (2005 2012) and Kevin Schey, PhD,

More information

Small Molecule Drug Imaging of Mouse Tissue by MALDI-TOF/TOF Mass Spectrometry and FTMS

Small Molecule Drug Imaging of Mouse Tissue by MALDI-TOF/TOF Mass Spectrometry and FTMS Bruker Daltonics Application Note # MT-93/FTMS-38 Small Molecule Drug Imaging of Mouse Tissue by MALDI-TOF/TOF Mass Spectrometry and FTMS Introduction Matrix Assisted Laser Desorption Ionization (MALDI)

More information

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE

More information

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Ionization source. Mass analyzer. Detector.

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Ionization source. Mass analyzer. Detector. Mass Analyzers Árpád Somogyi OSU, Summer Workshop August 18, 2015 Want to do MS or MS/MS? Need a Mass Spectrometer Ionization source Mass analyzer Detector inlet all ions sorted ions Data system 1 Ionization

More information

Characterization of Noncovalent Complexes of Polyelectrolytes by Mass Spectrometry

Characterization of Noncovalent Complexes of Polyelectrolytes by Mass Spectrometry The University of Akron IdeaExchange@UAkron Honors Research Projects The Dr. Gary B. and Pamela S. Williams Honors College Spring 2015 Characterization of Noncovalent Complexes of Polyelectrolytes by Mass

More information

Quantitation of Spatially-Localized Protein in Tissue Samples using MALDI-MRM Imaging. Elizabeth J. Clemis BSc, University of Victoria, 2008

Quantitation of Spatially-Localized Protein in Tissue Samples using MALDI-MRM Imaging. Elizabeth J. Clemis BSc, University of Victoria, 2008 Quantitation of Spatially-Localized Protein in Tissue Samples using MALDI-MRM Imaging by Elizabeth J. Clemis BSc, University of Victoria, 2008 A Thesis Submitted in Partial Fulfillment of the Requirements

More information

Mass Spectrometry. Actual Instrumentation

Mass Spectrometry. Actual Instrumentation Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY

More information

Mass spectrometry based proteomics

Mass spectrometry based proteomics Practice-oriented, student-friendly modernization of the biomedical education for strengthening the international competitiveness of the rural Hungarian universities TÁMOP-4.1.1.C-13/1/KONV-2014-0001 Mass

More information

Proteins: Proteomics & Protein-Protein Interactions Part I

Proteins: Proteomics & Protein-Protein Interactions Part I Proteins: Proteomics & Protein-Protein Interactions Part I Jesse Rinehart, PhD Department of Cellular & Molecular Physiology Systems Biology Institute DNA RNA PROTEIN DNA RNA PROTEIN Proteins: Proteomics

More information

The Detection of Allergens in Food Products with LC-MS

The Detection of Allergens in Food Products with LC-MS The Detection of Allergens in Food Products with LC-MS Something for the future? Jacqueline van der Wielen Scope of Organisation Dutch Food and Consumer Product Safety Authority: Law enforcement Control

More information

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Kai Scheffler, PhD BioPharma Support Expert,LSMS Europe The world

More information

MALDI-IMS (MATRIX-ASSISTED LASER DESORPTION/IONIZATION

MALDI-IMS (MATRIX-ASSISTED LASER DESORPTION/IONIZATION MALDI-IMS (MATRIX-ASSISTED LASER DESORPTION/IONIZATION IMAGING MASS SPECTROMETER) IN TISSUE STUDY YANXIAN CHEN MARCH 8 TH, WEDNESDAY. SEMINAR FOCUSING ON What is MALDI imaging mass spectrometer? How does

More information

Research to Routine Workflows for Large and Small Molecules using the Q Exactive HR/MS

Research to Routine Workflows for Large and Small Molecules using the Q Exactive HR/MS Research to Routine Workflows for Large and Small Molecules using the Q Exactive HR/MS Sept. 22, 2011 Patrick Bennett Director Pharma Strategic Marketing Thermo Fisher Scientific Agenda Definitions Research

More information

Tissue and Fluid Proteomics Chao-Cheng (Sam) Wang

Tissue and Fluid Proteomics Chao-Cheng (Sam) Wang Tissue and Fluid Proteomics Chao-Cheng (Sam) Wang UAB-03/09/2004 What is proteomics? A snap shot of the protein pattern!! What can proteomics do? To provide information on functional networks and/or involvement

More information

Advances in Hybrid Mass Spectrometry

Advances in Hybrid Mass Spectrometry The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap

More information

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Summer Workshop. Mass analyzer. Ionization source.

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Summer Workshop. Mass analyzer. Ionization source. Mass Analyzers Árpád Somogyi Summer Workshop Want to do MS or MS/MS? Need a Mass Spectrometer Ionization source Mass analyzer Detector inlet all ions sorted ions Data system 1 Ionization source Mass analyzer

More information

ABSTRACT. Catherine Fenselau, Professor, Department of Chemistry and Biochemistry

ABSTRACT. Catherine Fenselau, Professor, Department of Chemistry and Biochemistry ABSTRACT Title of Dissertation: ANALYSIS OF INTACT PROTEINS IN COMPLEX MIXTURES Avantika Dhabaria Doctor of Philosophy 2013 Directed By: Catherine Fenselau, Professor, Department of Chemistry and Biochemistry

More information

On the Nature of the Chemical Noise in MALDI Mass Spectra

On the Nature of the Chemical Noise in MALDI Mass Spectra On the Nature of the Chemical Noise in MALDI Mass Spectra Andrew N. Krutchinsky and Brian T. Chait* Rockefeller University, New York, New York, USA The so-called chemical noise background imposes a major

More information

Summary Chapter 8 CHAPTER 8. Summary. Page 173

Summary Chapter 8 CHAPTER 8. Summary. Page 173 CHAPTER 8 Summary Page 173 Chapter 2: Liquid Chromatography/Tandem Mass Spectrometry Method for Quantitative Estimation of PEG 400 and its Applications A rapid sensitive and selective MRM based method

More information

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses

More information

Ionization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)

Ionization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s) Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization

More information

FOURIER TRANSFORM MASS SPECTROMETRY

FOURIER TRANSFORM MASS SPECTROMETRY FOURIER TRANSFORM MASS SPECTROMETRY https://goo.gl/vx3ogw FT-ICR Theory Ion Cyclotron Motion Inward directed Lorentz force causes ions to move in circular orbits about the magnetic field axis Alan G. Marshall,

More information

(III) MALDI instrumentation

(III) MALDI instrumentation Dr. Sanjeeva Srivastava (I) Basics of MALDI-TF (II) Sample preparation In-gel digestion Zip-tip sample clean-up Matrix and sample plating (III) MALDI instrumentation 2 1 (I) Basics of MALDI-TF Analyte

More information

Agilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value

Agilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value Agilent 64 Triple Quadrupole LC/MS Sensitivity, Reliability, Value Sensitivity, Reliability, Value Whether you quantitate drug metabolites, measure herbicide levels in food, or determine contaminant levels

More information

Mass Spectrometry Infrastructure

Mass Spectrometry Infrastructure Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical

More information

INTRODUCTION TO MALDI IMAGING

INTRODUCTION TO MALDI IMAGING INTRODUCTION TO MALDI IMAGING Marten F. Snel, Emmanuelle Claude, Thérèse McKenna, and James I. Langridge Waters Corporation, Manchester, UK INT RODUCTION The last few years have seen a rapid increase in

More information

SUPPORTING INFORMATION:

SUPPORTING INFORMATION: 1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld

More information

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics July.3.13 Ken Miller Vice President of Marketing, Life Sciences Mass Spectrometry 1 The world leader in serving science Omics & the

More information

Molecular Cartography:

Molecular Cartography: Molecular Cartography: Moving Towards Combined Topographical and Chemical Imaging Using AFM and Mass Spectrometry Olga S. Ovchinnikova Organic and Biological Mass Spectrometry Group, Chemical Sciences

More information

Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices

Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring

More information

Mass Spectrometry and Proteomics. Professor Xudong Yao Bioanalytical Chemistry Spring 2007

Mass Spectrometry and Proteomics. Professor Xudong Yao Bioanalytical Chemistry Spring 2007 Mass Spectrometry and Proteomics Professor Xudong Yao Bioanalytical Chemistry Spring 2007 Proteomics and -omics Roles of mass spectrometry Comparative proteomics Chemical proteomics Protein, Proteome and

More information

Chapter 3. Structure of Enzymes. Enzyme Engineering

Chapter 3. Structure of Enzymes. Enzyme Engineering Chapter 3. Structure of Enzymes Enzyme Engineering 3.1 Introduction With purified protein, Determining M r of the protein Determining composition of amino acids and the primary structure Determining the

More information

Mass spectrometry Technologies in Lipid chemistry

Mass spectrometry Technologies in Lipid chemistry Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to

More information

Phosphorylation of proteins Steve Barnes Feb 19th, 2002 in some cases, proteins are found in a stable, hyperphosphorylated state, e.g.

Phosphorylation of proteins Steve Barnes Feb 19th, 2002 in some cases, proteins are found in a stable, hyperphosphorylated state, e.g. Phosphorylation of proteins Steve Barnes Feb 19th, 2002 in some cases, proteins are found in a stable, hyperphosphorylated state, e.g., casein more interestingly, in most other cases, it is a transient

More information

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management CONTENT Chapter No. Title Page No. Abbreviations List of Figures List of Tables 1 Introduction 1 1.1 Practical aspects 4 1.2 Stability-indicating assay method (SIAM) 5 1.3 Regulatory aspects 6 1.4 Techniques

More information

Introduction to LC/MS/MS

Introduction to LC/MS/MS Trends in 2006 Introduction to LC/MS/MS By Crystal Holt, LC/MS Product Specialist, Varian Inc. Toxicology laboratories Increased use of LC/MS Excellent LD Cheaper (still expensive) Much more robust Solves

More information

MALDI IMAGING MASS SPECTROMETRY FOR SMALL MOLECULE QUANTITATION AND EVALUATION FOR MARKERS OF DRUG RESPONSE IN GLIOMAS. Sara L. Frappier.

MALDI IMAGING MASS SPECTROMETRY FOR SMALL MOLECULE QUANTITATION AND EVALUATION FOR MARKERS OF DRUG RESPONSE IN GLIOMAS. Sara L. Frappier. MALDI IMAGING MASS SPECTROMETRY FOR SMALL MOLECULE QUANTITATION AND EVALUATION FOR MARKERS OF DRUG RESPONSE IN GLIOMAS By Sara L. Frappier Dissertation Submitted to the Faculty of the Graduate School of

More information

Mass Analyzers. Árpád Somogyi. Campus Chemical Instrument Center Ohio State University. OSU, July 13, What is Mass Spectrometry?

Mass Analyzers. Árpád Somogyi. Campus Chemical Instrument Center Ohio State University. OSU, July 13, What is Mass Spectrometry? Mass Analyzers Árpád Somogyi Campus Chemical Instrument Center Ohio State University OSU, July 13, 2016 What is Mass Spectrometry? Mass spectrometry is a powerful tool in analytical/bioanalytical chemistry

More information

Characterization and Quantification of Covalent Modification of Proteins Using Mass Spectrometry

Characterization and Quantification of Covalent Modification of Proteins Using Mass Spectrometry City University of New York (CUNY) CUNY Academic Works Dissertations, Theses, and Capstone Projects Graduate Center 9-2018 Characterization and Quantification of Covalent Modification of Proteins Using

More information

QUANTITATIVE ANALYSIS IN PROTEOMICS AND METABOLOMICS

QUANTITATIVE ANALYSIS IN PROTEOMICS AND METABOLOMICS UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II TESI DI DOTTORATO IN SCIENZE CHIMICHE XXVIII CICLO QUANTITATIVE ANALYSIS IN PROTEOMICS AND METABOLOMICS Francesca Pane Tutor Prof. Angela Amoresano Coordinatore

More information

SYNAPT G2-S High Definition MS (HDMS) System

SYNAPT G2-S High Definition MS (HDMS) System SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business

More information

Go beyond. to realities unexplored. Comprehensive workflows, integrated solutions

Go beyond. to realities unexplored. Comprehensive workflows, integrated solutions Go beyond to realities unexplored Comprehensive workflows, integrated solutions We re committed to helping researchers and scientists in academia and industry harness the power of metabolomics to gain

More information

PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012

PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012 Courtney Phillips Wednesday, March 14, 2012 courtney.phillips@perkinelmer.com 1 In 1996, the FQPA amended the two federal laws governing pesticides Federal Insecticide, Fungicide FIFRA& Rodenticide Act

More information

Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications

Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications Triple Stage Quadrupole API MS / MS Full Scan Products / - IONS AND NEUTRALS FORMED IN API SOURCE Q0 LENS TRANSPORTS

More information

Tandem Mass Spectrometric Analysis of Bacterial Lipid A of Aeromonas Salmonicida (SJ-112)

Tandem Mass Spectrometric Analysis of Bacterial Lipid A of Aeromonas Salmonicida (SJ-112) Tandem Mass Spectrometric Analysis of Bacterial Lipid A of Aeromonas Salmonicida (SJ-112) by: Faisal Alarabi A thesis submitted to the School of Graduate Studies in partial fulfillment of the requirements

More information

Terminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids

Terminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Metabolome: the quantitative complement of all the low molecular weight molecules present in

More information

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations

More information

Method Development for Characterization of N- linked Glycoproteins in Mass Spectrometry

Method Development for Characterization of N- linked Glycoproteins in Mass Spectrometry Method Development for Characterization of N- linked Glycoproteins in Mass Spectrometry By 2007 Ying Zhang Ph.D., University of Kansas 2007 Submitted to the Department of Chemistry and the Faculty of the

More information

UNIVERSITÀ DEGLI STUDI DI PARMA

UNIVERSITÀ DEGLI STUDI DI PARMA UNIVERSITÀ DEGLI STUDI DI PARMA Dottorato di ricerca in Progettazione e Sintesi di Composti Biologicamente Attivi Ciclo XXVII Mass spectrometry in structure-property relationships studies: allosteric inhibitors

More information

Mass Spectrometry in Clinical Chemistry. David Hardy

Mass Spectrometry in Clinical Chemistry. David Hardy Mass Spectrometry in Clinical Chemistry David Hardy What is Mass Spectrometry? The separation of ions on the basis of mass to charge ratio (m/z) Gas phase technique requiring high vacuum Non-volatile compounds

More information