ACE Excel UHPLC Columns

Size: px
Start display at page:

Download "ACE Excel UHPLC Columns"

Transcription

1 Excel UHPLC Columns Advanced Chromatography Technologies Ltd Australia & New Zealand contact: or call + 0

2 Outline Introduction Key Product Points Examples of Isocratic and Gradient HPLC UHPLC Key Product Characteristics Fixtures and Fittings Australia & New Zealand contact: or call + 0

3 Key Excel Features Available as NEW high efficiency, ultra-inert mm silica particles and suitable for UHPLC at 000bar (000psi) Nine selectivities including the unique C8-AR and C8-PFP Highly reproducibility: column-to-column and batch-to-batch Ultra-robust phases: NEW low dispersion column hardware and NEW High Stability Column (HSC ) packing technology Engineered with lower back pressures compared to other <mm phases due to mm particle size and frit technology Fully scalable to mm, mm and 0mm (Usable at lower pressures giving a performance boost to HPLC) Australia & New Zealand contact: infor@winlab.com.au or call + 0

4 Using Excel C8-PFP Selectivity & Throughput (Isocratic) Aim: obtain R s. in shortest possible time for mixture Waters XBridge C8 0 x. mm.00 ml/min R s =. HPLC: µm C8 t r =.0 mins (L/d p = ) Waters ACQUITY. BEH C8 0 x. mm 0. ml/min R s =. UHPLC: <µm C8 t r =.9 mins (L/d p =.9) To maintain R s and reduce run time, keep L / d p ratio constant ~ x Quicker Excel C8-PFP 0 x. mm.0 ml/min UHPLC: Excel µm C8-PFP t r =0. mins R s =. R s =.9 Using UHPLC and selectivity, it is possible to dramatically improve resolution allowing shorter columns & increased flow rates Sample: ),,dimethoxybenzene, ),-dimethoxybenzene, ),,-trimethoxybenzene, ) toluene (reference). Mobile phase 0:0 MeOH / H O; Temperature 0 C All trademarks are recognised Australia & New Zealand contact: infor@winlab.com.au or call + 0

5 Using Excel C8-PFP Selectivity & Throughput (Gradient) Aim: obtain R s. in shortest possible time for mixture C8 00 x. mm ml/min, t G = 9 min max pressure: 9 bar 0 min cycle time R s =.8,.9 min HPLC: µm C8.9 mins Excel C8 0 x. mm 0. ml/min, t G = min max pressure: bar 9 min cycle time Excel C8-PFP 0 x. mm. ml/min, tg = 0. min max pressure: 9 bar min cycle time 0 0 R s =.8 0 R s =., 0. min R s =.9 R s =. 0.. R s =. UHPLC: µm C8-PFP 0. mins,. min UHPLC: <µm C8. mins... ~ x Quicker min R s , aspirin;, phenacetin;,,-dinitrobenzene ;, ethylbenzoate;, nimesulide;, ibuprofen;, indomethacin. min Australia & New Zealand contact: infor@winlab.com.au or call + 0

6 Excel Has Typically Lower Back Pressure For UHPLC Specifically engineered for lower UHPLC backpressures Excel C8-AR P max : bar Waters ACQUITY.um BEH C P max : 8 bar Phenomenex Kinetex.um C8, P max : 0 bar Agilent ZORBAX Eclipse.8 um XDB C8, P max : 0 bar min 0x.mm; conditions: A=mM formic acid (aq); B=mM formic acid in MeOH; tg= to 00%B in min; 0. ml/min; 0C; nm. Paracetamol. Hydrochlorothiazide. Methylphenylsulphoxide. Methylphenylsulphone. Aspirin. Phenacetin.,-dinitrobenzene 8.,,-trimethoxybenzene 9. Ethylbenzoate 0. Nimesulide. Ibuprofen. Indomethacin. Mefenamic acid All trademarks are recognised Australia & New Zealand contact: infor@winlab.com.au or call + 0

7 Excel UHPLC Columns Scalability & Reproducibility Excel C8 0 x.mm 0.mL/min µm UHPLC C8 0 x.0mm 0.0mL/min µm HPLC C8 0 x.mm.00ml/min µm 0 C8 0 x.mm.ml/min 0 µm 0 8 Prep LC MP: : v/v MeCN:0.% TFA (aq); C; nm;. uracil;. -hydroxybenzoic acid;. acetylsalicyclic acid;. benzoic acid;. -hydroxybenzoic acid. ethyl paraben Australia & New Zealand contact: infor@winlab.com.au or call + 0

8 Excel UHPLC Column Robustness 000 bar for ~000 gradient runs Isocratic efficiency assessments every ~00 runs more demanding! Excel C8 (with HSC Packing Technology) Prototype mm C8 (without HSC Packing Technology) 00x.mm; MPA 0.% FA (aq); MPB: 0.% FA in MeOH; 0.mL/min; gradient: 0-90%B in mins, min hold; min re-equilibration. NEW High Stability Column (HSC ) Packing Technology Significantly Improves UHPLC Column Robustness 8 Australia & New Zealand contact: infor@winlab.com.au or call + 0

9 Excel UHPLC Columns ph and Temperature Stability: For optimal column lifetime, as with standard columns, the recommended ph operating range is -8 Excel columns can be used outside of this ph range but column lifetimes will vary with a number of factors (buffer, temperature, solvents etc)* Excel columns can be operated at temperatures up to 00 C (but temperatures >0 C will reduce column lifetimes) * For further information see FREE booklet: A Guide to HPLC and LC-MS Buffer Selection Australia & New Zealand contact: infor@winlab.com.au or call + 0

10 Excel C8-AR: Water Soluble Vitamins - Selectivity Excel C8-AR P max : 09 bar, Excel C8 P max : bar Agilent ZORBAX Eclipse XDB-C8 P max : 99 bar Waters ACQUITY BEH C8 P max : bar Phenomenex Kinetex. C8, P max : bar 0x.mm; MP A: 0 mm KH PO, ph.; MPB: 0 mm KH PO, ph. in MeOH/H O (0:0 v/v); Gradient: 0 0 %B in min; 0. ml/min; 0 C; 0 nm. Pyridoxine;. P-aminobenzoic acid;. Pantothenic acid;. Folic acid;. D-Biotin;. Cyanocobalamin;. Riboflavin min Australia & New Zealand contact: infor@winlab.com.au or call + 0

11 Excel C8-PFP: Structural Isomers - Selectivity Excel C8-PFP P = bar,, Excel C8, P = 0 bar,, Waters. ACQUITY BEH C8, P = bar,, Phenomenex Kinetex. C8, P = bar,, Agilent Poroshell 0 EC-C8, P = 08 bar 8 9 min 0x.mm; MP: MeOH/H O (0:0 v/v); 0. ml/min; 0 C; 0/ nm;.,,-trimethoxybenzene;.,-dimethoxybenzene;.,,-trimethoxybenzene;. methoxybenzene;.,-dimethoxybenzene;.,,-trimethoxybenzene;. toluene Australia & New Zealand contact: infor@winlab.com.au or call + 0

12 Excel UHPLC Columns A reusable UHPLC column connector is available EXL-CC EXL-CC0 = / pk = 0 / pk Rated to 0 bar (,000 psi), these are suitable for at least 0 make / break cycles when correctly fitted Suitable for use at temperature up to 00C A torque wrench is recommended to ensure the correct force is applied EXL-TW = / pk Australia & New Zealand contact: infor@winlab.com.au or call + 0

13 Excel UHPLC Columns A starter kit containing x Torque Wrench and x reusable connectors is available EXL-CCSK

Exploiting Selectivity in HPLC and UHPLC With Rational Stationary Phase Design

Exploiting Selectivity in HPLC and UHPLC With Rational Stationary Phase Design Exploiting Selectivity in HPLC and UHPLC With Rational Stationary Phase Design Alan P McKeown amckeown@ace-hplc.com Advanced Chromatography Technologies www.ace-hplc.com www.mac-mod.com Outline 2 Chromatographic

More information

ACE C18-AR. A C18 bonded phase with unique selectivity. Guaranteed reproducibility. Exceptional bonded phase stability

ACE C18-AR. A C18 bonded phase with unique selectivity. Guaranteed reproducibility. Exceptional bonded phase stability ACE C8-AR A C8 bonded phase with unique selectivity Guaranteed reproducibility Exceptional bonded phase stability Hydrophobic and aromatic mixed mode interaction High efficiency µm, µm, µm and 0µm particles

More information

ACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development

ACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency

More information

ACE CN-ES. Extra Stability. Extended Spacer. Enhanced Selectivity ACE. Combining CN polar selectivity with enhanced hydrophobicity

ACE CN-ES. Extra Stability. Extended Spacer. Enhanced Selectivity ACE. Combining CN polar selectivity with enhanced hydrophobicity ACE CN-ES Combining CN polar selectivity with enhanced hydrophobicity Extended Spacer Extra Stability New stationary phase technology for UHPLC and HPLC separations Alternative polar selectivity for method

More information

ACE C18-AR Use the Power!

ACE C18-AR Use the Power! combines the retention and separation characteristics of a C18 phase with the extra selectivity of an integral phenyl functional group. The combination provides extra resolving power for a wide variety

More information

Aeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE

Aeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance

More information

ACE ACE. HPLC and UHPLC Columns. Product Catalogue

ACE ACE. HPLC and UHPLC Columns. Product Catalogue ACE HPLC and UHPLC Columns Product Catalogue ACE Ako nás možno kontaktova : AZ Chrom s.r.o. Robotnícka 8 Bratislava Tel. 97 Fax. 78 azetchrom@hplc.sk www.azetchrom.sk À ÆøÛ ªÆ Ù fiø ª ªø ø ªº ÿ Àÿ ± Ô

More information

ACE ACE. HPLC and UHPLC Columns. Product Catalog

ACE ACE. HPLC and UHPLC Columns. Product Catalog ACE HPLC and UHPLC Columns Product Catalog ACE Ultra-Inert, Base Deactivated HPLC & UHPLC Columns ACE Ultra-Inert, Base Deactivated HPLC and ACE Excel UHPLC columns give you the choices you need to achieve

More information

ACE. Intelligent Solutions for Method Development. Method Development Kits. ACE UHPLC and HPLC Columns. a a

ACE. Intelligent Solutions for Method Development. Method Development Kits. ACE UHPLC and HPLC Columns. a a r: ff e ts ice O ki r ti y mn e p tiv olu sam mn c c u le the col e d S an for ngle le si ab s a l i a a av ACE Method Development Kits Intelligent Solutions for Method Development Highly cost effective

More information

Am I getting the very best value from my UHPLC analyses?

Am I getting the very best value from my UHPLC analyses? Am I getting the very best value from my UHPLC analyses? Stephen Luke LC Columns Product Manager 1 Primary reasons for UHPLC use Very fast Very high resolution Column 2.1 x 50 mm Column 2.1 x 150 mm Run

More information

Restek Ultra II HPLC Columns

Restek Ultra II HPLC Columns Restek Ultra II HPLC Columns THE Choice for All U(HPLC) Systems November 2009 1 www.chromtech.net.au, sales@chromtech.net.au, Tel: 03 9762 2034, Fax: 03 9761 1169 Topics for Today Introducing Restek Ultra

More information

APPLICATIONS TN µm Core-Shell Particle. (continued on page 2) Page 1 of 8. For additional technical notes, visit

APPLICATIONS TN µm Core-Shell Particle. (continued on page 2) Page 1 of 8. For additional technical notes, visit Kinetex 5 µm Core-Shell Columns Deliver Significant Improvements in Chromatographic Efficiency and Resolution versus Traditional Fully Porous 5 µm Columns Philip J. Koerner and Jeff Layne Phenomenex, Inc.,

More information

The LC Column Playbook

The LC Column Playbook The LC Column Playbook Mark Powell Applications Engineer Technical Support 1 Overview How do we achieve our goal: Resolution? Efficiency Particle size Column length Selectivity Bonded phase Mobile phase

More information

Dikma Technologies Inc.

Dikma Technologies Inc. Dikma HPLC / UHPLC Columns Selection Guide HPLC Column Selection Chart Sample MW Sample Solubility Separation Mode Dikma Recommended Column Page Fat Soluble Normal Phase Platisil NH 2 Platisil CN Platisil

More information

High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development

High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development Robert Ricker Bio-Applications Scientist CABU April 5, 2 Rapid Resolution HPLC High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development Slide 4 Ultra-Fast

More information

ACE HILIC + + UHPLC and HPLC Columns. For Reproducible Polar Analyte Separations. Three HILIC Column Chemistries Available

ACE HILIC + + UHPLC and HPLC Columns. For Reproducible Polar Analyte Separations. Three HILIC Column Chemistries Available ACE HILIC UHPLC and HPLC s For Reproducible Polar Analyte Separations Three HILIC Chemistries Available ACE HILIC s What is HILIC? Hydrophilic Interaction Liquid Chromatography (HILIC) was first described

More information

APPLICATIONS TN Overview of Kinetex 2.6 µm Core-Shell Technology

APPLICATIONS TN Overview of Kinetex 2.6 µm Core-Shell Technology Determination of Impurities and Related Substances for Acetylsalicylic Acid (Ph. Eur. Monograph 9): Increased Sensitivity and Resolution, Faster Analysis, and Reduced Solvent Usage Using Kinetex 2.6 µm

More information

USP Method Transfer of Ziprasidone HCl from HPLC to UPLC

USP Method Transfer of Ziprasidone HCl from HPLC to UPLC Mia Summers and Kenneth J. Fountain Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Updating USP Methods from HPLC to UPLC using sub-2 µm columns 85% decrease in analysis time, faster throughput

More information

APPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology.

APPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology. Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology Jeff Layne, Phenomenex, Inc. Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 951 USA With virtually

More information

Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system

Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author

More information

Comparison of a UPLC Method across Multiple UHPLC Systems

Comparison of a UPLC Method across Multiple UHPLC Systems Comparison of a UPLC Method across Multiple UHPLC Systems Tanya Jenkins Waters Corporation, Milford, MA, U.S. INTRODUCTION In 2004, Waters introduced the ACQUITY UPLC System. Since this launch, many liquid

More information

2012 Waters Corporation 1

2012 Waters Corporation 1 An Introduction to Waters HPLC and ACQUITY UPLC Column Technologies for small molecule analysis Esa Lehtorinne Esa_Lehtorinne@waters.com Tel: +358-9-5659 5659 6288 Fax: +358-9-5659 5659 6282 Waters Finland

More information

New HPLC Column Options for UHPLC

New HPLC Column Options for UHPLC New HPLC Column Options for UHPLC Rapid Resolution High Definition Columns and More Chor Koon Tan, PhD Business Development Manager Columns & Supplies Division Asia-Pacific Agilent Technologies Page 1

More information

Dikma Technologies Inc.

Dikma Technologies Inc. Dikma HPLC / UHPLC Columns Selection Guide Dikma Technologies Inc. www.dikmatech.com HPLC Column Selection Chart Sample MW Sample Solubility Separation Mode Dikma Recommended Column Fat Soluble Normal

More information

For Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns.

For Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns. For Far Greater UHPLC Column Performance... You Must Have Kinetex Core-Shell UHPLC Columns www.phenomenex.com/kinetex The UHPLC Core-Shell Advantage Kinetex Core-Shell Technology produces increased efficiencies

More information

Thermo Scientific Hypersil BDS Columns

Thermo Scientific Hypersil BDS Columns Technical Guide Version 2 Thermo Scientific Hypersil BDS Columns Robust and rugged, delivering excellent reproducibility and peak symmetry Part of Thermo Fisher Scientific Thermo Scientific Hypersil BDS

More information

Agilent Technologies Prep LC Columns

Agilent Technologies Prep LC Columns Agilent Technologies Prep LC Columns Agilent Technologies Prep LC Columns Agilent Technologies has always taken seriously its responsibility to ensure your success. That s why all our instruments and supplies

More information

UHPLC / HPLC Columns Developed for Mass Spectrometry

UHPLC / HPLC Columns Developed for Mass Spectrometry ACE UltraCore SuperC18 UHPLC / HPLC s Developed for Mass Spectrometry Water analysis Pharmaceutical Environmental Toxicology Food safety Clinical Forensics Metabolism Trace analysis Ultra-inert 2.5µm and

More information

Core-Shell Technology for Proteins and Peptides

Core-Shell Technology for Proteins and Peptides Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein

More information

Phenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases

Phenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases Phenyl-Hexyl UHPLC Columns lternate, complementary selectivity to A C8 and C8 bonded phases articularly recommended for P compounds containing aromatic groups xcellent bonded phase stability for E durable,

More information

Preparative Chromatography Meets UHPLC

Preparative Chromatography Meets UHPLC Preparative Chromatography Meets UHPLC The Power of Kinetex Core-Shell Technology with the Performance of Piston Packed Prep Columns Ultra-High Efficiencies Ultra-Long Lifetimes NO Added Pressure Two Award

More information

Meteoric Core. Core-Shell Columns for UHPLC & HPLC. Easy Method Optimization! Excellent Chromatography with Fast Separation!

Meteoric Core. Core-Shell Columns for UHPLC & HPLC. Easy Method Optimization! Excellent Chromatography with Fast Separation! Meteoric Core Easy Method Optimization! Excellent Chromatography with Fast Separation! Core Porous shell layer Meteoric Core Particle Image Structure Schöttmannshof 19 46539 Dinslaken Phone +49 (0)2064

More information

11:00 a.m. EST Telephone Number: Chair Person: Tim Spaeder

11:00 a.m. EST Telephone Number: Chair Person: Tim Spaeder Stephen W. Coates LC Columns Marketing Specialist Consumables and Accessories Business Unit May 17, 2 High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry, and Rapid Method Development

More information

InertSustainBio C18. Rapid Separations of Proteins and Peptides

InertSustainBio C18. Rapid Separations of Proteins and Peptides InertSustainBio C18 Rapid Separations of Proteins and Peptides High Recoveries of Proteins and Peptides The 200A pore size silica creates the opportunity to separate compounds having a molecular weight

More information

The One Minute Chromatographer

The One Minute Chromatographer The One Minute Chromatographer Agilent Technologies, Inc. 2013 Page 1 Sub 1 Minute Separations with RRHD Columns Agilent ZORBAX RRHD SB-C18, 2.1 x 100mm, 1.8 µm H2O (0.05% Formic acid) / 2-98 % Acetonitrile

More information

Maximizing Efficiency Using Agilent InfinityLab Poroshell 120 Columns

Maximizing Efficiency Using Agilent InfinityLab Poroshell 120 Columns Maximizing Efficiency Using Agilent InfinityLab Poroshell 12 Columns 1, Plates in Less Than Minutes Using Coupled Column Technology Application Note Food, Environmental, Chemical, Pharmaceutical Authors

More information

InertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior

InertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups,

More information

Yes You Can! New! Amazing EVO C18. Core-Shell Technology

Yes You Can! New! Amazing EVO C18. Core-Shell Technology Core-Shell Technology Yes You Can! New! Amazing EVO C8 Develop robust methods from ph - Get improved peak shape for bases Easily reduce run times and increase sensitivity THE PAST Fully Porous You used

More information

Effective Surface Area (m 2 /g) Pore Size (Å)

Effective Surface Area (m 2 /g) Pore Size (Å) n e w Ultra-High Performance on Any LC System Achieve sub- µm performance within HPLC backpressure limitations Substitute µm and µm columns for -x higher efficiency Obtain higher throughput without sacrificing

More information

Columns The FIRST and ONLY sub-2 µm core-shell columns on the market

Columns The FIRST and ONLY sub-2 µm core-shell columns on the market Better UHPLC Performance Starts NW.7 µm Core-Shell * Columns The IRST and NLY sub- µm core-shell columns on the market Phenomenex India Laxmi Cyber City, Ground loor, B Block, Survey no: Kondapur, Hyderabad

More information

Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate

Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,

More information

Ultimate Core Performance to Maximize Your Investment

Ultimate Core Performance to Maximize Your Investment Thermo Scientific Accucore HPL olumns Phase Overview Ultimate ore Performance to Maximize Your Investment Phase Overview Founded on state-of-the-art ore Enhanced Technology and utilizing vast experience

More information

APPLICATIONS Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns

APPLICATIONS Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns Michael McGinley, Deborah Jarrett, and Jeff Layne Phenomenex, Inc., 411 Madrid Avenue, Torrance, CA 91 USA Aeris WIDEPORE

More information

Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System

Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 129 Infinity LC System Application Note Pharmaceutical and Chemical Analysis

More information

Hyper-fast & Super-rugged

Hyper-fast & Super-rugged Hyper-fast & Super-rugged UHPLC & HPLC columns FALL 2013 PRODUCT CATALOG Hyper-fast & Super-rugged UHPLC & HPLC columns he introduction of HALO columns, developed using innovative Fused-Core particle

More information

SeQuant ZIC -HILIC For all who expect more...

SeQuant ZIC -HILIC For all who expect more... SeQuant ZIC -ILIC For all who expect more... The better choice for PLC and LC/MS of all types of polar and hydrophilic compounds EMD Millipore is a division of Merck KGaA, Darmstadt, Germany Poor retention

More information

Awesome! Unconventionally. Kinetex EVO C18 HPLC/UHPLC Core-Shell Columns 2.6. Core-Shell Technology

Awesome! Unconventionally. Kinetex EVO C18 HPLC/UHPLC Core-Shell Columns 2.6. Core-Shell Technology Core-Shell Technology Kinetex EVO C8 HPLC/UHPLC Core-Shell Columns Greater Polar Retention with % Aqueous Stability Robust Methods from ph - Improved Peak Shape for Bases NEW.7 µm NEW. µm µm Unconventionally

More information

Rapid and sensitive UHPLC screening for water soluble vitamins in sports beverages

Rapid and sensitive UHPLC screening for water soluble vitamins in sports beverages APPLICATION NOTE 21671 Rapid and sensitive UHPLC screening for water soluble vitamins in sports beverages Authors Jon Bardsley, Thermo Fisher Scientific, Runcorn, UK Keywords Vanquish Flex, Acclaim PolarAdvantage

More information

HPLC Application Notes

HPLC Application Notes HPLC Application Notes Antibiotics. Ampicillin. Chloramphenicol Prestige C8 5µm, 50 x 4.6mm CAN/MeOH/(NH 4 )HPO 4 (0/0/70) UV at 0nm Injection Volume µl Amino Acids. L-Valine. L-Phenylalanine Polar Embedded

More information

Prep Columns. and Bulk Media. and Bulk Media

Prep Columns. and Bulk Media. and Bulk Media Prep Columns and Bulk Media Axia Columns...346-355 Process Chromatography...356-36 Bulk Media...356-36 Columns, Scout and Preparative...359-36 Sepra Bulk Sorbents... 36 Prep Columns and Bulk Media 345-36

More information

Core-Shell Technology. 11 HPLC Myths. Uncovered. & Why You Must Have Kinetex Core-Shell HPLC Columns

Core-Shell Technology. 11 HPLC Myths. Uncovered. & Why You Must Have Kinetex Core-Shell HPLC Columns Core-Shell Technology 11 HPLC Myths Uncovered & Why You Must Have Kinetex Core-Shell HPLC Columns 2.6 www.phenomenex.com/kinetex HPLC myths have been around since the early 19 s! From the one about the

More information

Thermo Scientific. GlycanPac AXR-1. Column Product Manual. P/N: April, Part of Thermo Fisher Scientific

Thermo Scientific. GlycanPac AXR-1. Column Product Manual. P/N: April, Part of Thermo Fisher Scientific Thermo Scientific GlycanPac AXR-1 Column Product Manual P/N: April, 2014 Part of Thermo Fisher Scientific Product Manual for GlycanPac AXR-1 Columns GlycanPac AXR-1, 1.9 µm, Analytical, 2.1 x 150 mm (P/N

More information

SUMMARY, CONCLUSION & RECOMMENDATIONS

SUMMARY, CONCLUSION & RECOMMENDATIONS 196 Chapter-5 SUMMARY, CONCLUSION & RECOMMENDATIONS 197 CHAPTER 5 5.1 Summary, Conclusion and Recommendations Summary and Conclusion are drawn based on the work carried out by the author on development

More information

LC Columns with Liquid Separation Cell Technology

LC Columns with Liquid Separation Cell Technology Obelisc LC Columns with Liquid Separation Cell Technology Obelisc HPLC Columns - Liquid Separation Cell Technology Introduction Obelisc HPLC columns are the latest, innovative columns from SIELC Technologies,

More information

Converting a CHP Method for Insulin to Agilent Poroshell 120 Columns

Converting a CHP Method for Insulin to Agilent Poroshell 120 Columns Converting a CHP Method for Insulin to Agilent Poroshell Columns Application Note Biopharm Author Rongjie Fu Agilent Technologies (Shanghai) Co., Ltd. 4 Ying Lun Road Shanghai 3 China Abstract A regulatory

More information

Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column

Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Application Note Pharmaceutical and Food Testing Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Author Lu Yufei Agilent Technologies, Inc. Abstract A liquid chromatographic

More information

APPLICATIONS TN A Comparison of Various Kinetex C18 Phases USP: L1

APPLICATIONS TN A Comparison of Various Kinetex C18 Phases USP: L1 APPLICATIONS A Comparison of Various Kinetex C18 Phases Zeshan Aqeel and Jeff Layne Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 91 USA Introduction When performing reversed phase LC separations, there

More information

SeQuant ZIC -HILIC For all who expect more...

SeQuant ZIC -HILIC For all who expect more... SeQuant ZIC -ILIC For all who expect more... The better choice for PLC and LC/MS of all types of polar and hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany Poor

More information

Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 120 PFP

Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 120 PFP Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 1 PFP Application Note Clinical Research Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd Abstract

More information

Eurospher II the logical choice. Physical properties of Eurospher II silica gel: Silica gel: ultra pure, > % Metal content: < 10 ppm

Eurospher II the logical choice. Physical properties of Eurospher II silica gel: Silica gel: ultra pure, > % Metal content: < 10 ppm HPLC SMB smometry Euroline Eurospher II Eurospher II the logical choice After more than years on the market, our Eurospher stationary phase has established a reputation for being a first-class packing

More information

SecurityGuard. Column Protection for UHPLC, HPLC to PREP. A Universal Guard Cartridge System. Increases HPLC Column Lifetime, Guaranteed!

SecurityGuard. Column Protection for UHPLC, HPLC to PREP. A Universal Guard Cartridge System. Increases HPLC Column Lifetime, Guaranteed! U.S. Patent No. 6, 162, 362 Column Protection for UHPLC, HPLC to PREP Protect HPLC columns and extend lifetime Virtually no change in chromatography Available in analytical, semi-prep, and preparative

More information

Analytical and Preparative SFC Columns

Analytical and Preparative SFC Columns Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...

More information

YMC-Actus. Columns for Semi-preparative HPLC. Selectivity Durability Loadability

YMC-Actus. Columns for Semi-preparative HPLC. Selectivity Durability Loadability YMC-Actus Columns for Semi-preparative HPLC Selectivity Durability Loadability Fast semi-preparative chromatography Semi-preparative chromatography is the link between analytical HPLC and preparative LC.

More information

Application Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.

Application Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2

More information

Fortis Method Development Options

Fortis Method Development Options Method Dev Fortis Method Development Options Choice of Stationary phase functionality Based on Ultra pure silica Reversed Phase (RP) and Normal Phase (NP) options Fortis Ho - Polar endcapped C8 - Increased

More information

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F

More information

Evaluation of UHPLC System Performance Under High Throughput Conditions. William Hedgepeth, Masatoshi Takahashi, Shimadzu Scientific Instruments

Evaluation of UHPLC System Performance Under High Throughput Conditions. William Hedgepeth, Masatoshi Takahashi, Shimadzu Scientific Instruments Evaluation of UHPLC System Performance Under High Throughput Conditions William Hedgepeth, Masatoshi Takahashi, Shimadzu Scientific Instruments Introduction The use of UHPLC technology has continued to

More information

The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns.

The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. Fast High peak capacity Low back pressure PROTEIN PEPTIDE UHPLC COLUMNS Put these new, high-performance

More information

Gerry Hendrickx Regional Sales Manager Central Europe. Developments in Waters Column Chemistries : BEH Technology

Gerry Hendrickx Regional Sales Manager Central Europe. Developments in Waters Column Chemistries : BEH Technology Gerry Hendrickx Regional Sales Manager Central Europe Developments in Waters Column Chemistries : BEH Technology XTerra Columns: First Generation Hybrid Particles Initial Launch 1999 Advantages of XTerra

More information

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit

Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent

More information

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 129 Infinity II LC Application Note Small Molecule Pharmaceuticals Authors Gerd Vanhoenacker, Mieke Steenbeke, Koen Sandra,

More information

Normal Phase HPLC Column COSMOSIL SL-II

Normal Phase HPLC Column COSMOSIL SL-II Normal Phase HPLC Column COSMOSIL SL-II High purity silica gel (>99.99%) with special treatment Suitable for preparative separation Ultra-pure silica gel of more than 99.99% purity is used for the COSMOSIL

More information

Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC

Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC Bàrbara Bagó Bilbao, 12 Julio 2012 2011 Waters Corporation 1 Agenda Column Manufacturing and Platform Definitions New HPLC & ACQUITY UPLC Column Chemistries

More information

Unequalled durability against water elution. % tr

Unequalled durability against water elution. % tr Unequalled durability against water elution 2 Revolutionary aqueous durability for aminopropyl phase 1 3 4 1. fructose (2.5mg/mL) 3. sucrose (2.5mg/mL) 500 hr 2. glucose 400 hr Aqueous to non-aqueous Normal

More information

ARC-18. Stationary Phase: Ahead of the Curve for Large, Multiclass Lists by Mass Spec. Pure Chromatography. Selectivity Accelerated.

ARC-18. Stationary Phase: Ahead of the Curve for Large, Multiclass Lists by Mass Spec. Pure Chromatography. Selectivity Accelerated. Ahead of the Curve for Large, Multiclass Lists by Mass Spec Selectivity Accelerated Stationary Phase: ARC-18 Si O Pure Chromatography www.restek.com/raptor The Raptor ARC-18 Column With Raptor LC columns,

More information

A New Era in SFC Column Technology

A New Era in SFC Column Technology B TM A ew Era in SFC Column Technology Combining state-of-the-art media manufacturing with the industry leading column technology, Viridis Supercritical Fluid Chromatography (SFC) columns bring a new level

More information

Performance Characteristics of the Agilent 1290 Infinity II Multicolumn Thermostat

Performance Characteristics of the Agilent 1290 Infinity II Multicolumn Thermostat Performance Characteristics of the Agilent 9 Infinity II Multicolumn Thermostat Technical Overview Author Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany Abstract This Technical Overview

More information

Protect your expensive HPLC and UHPLC columns from premature failure with MAC-MOD Pre-Column Filters

Protect your expensive HPLC and UHPLC columns from premature failure with MAC-MOD Pre-Column Filters Protect your expensive HPLC and UHPLC columns from premature failure with MAC-MOD Pre-Column Filters Over 70% of the failures of HPLC columns are caused by inlet frit plugging. MAC-MOD Pre-Column Filters

More information

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Evelyn Goh Waters Pacific, Singapore APPLICATION BENEFITS This method allows for the simultaneous analysis of 12 water-soluble

More information

ANALYTICAL SCIENCES FEBRUARY 2014, VOL The Japan Society for Analytical Chemistry

ANALYTICAL SCIENCES FEBRUARY 2014, VOL The Japan Society for Analytical Chemistry ANALYTICAL SCIENCES FEBRUARY 2014, VOL. 30 205 2014 The Japan Society for Analytical Chemistry Special Reviews New Trend in the LC Separation Analysis of Pharmaceuticals High Performance Separation by

More information

Selectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants

Selectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants Selectivity Comparison of Agilent Poroshell 1 Phases in the Separation of Butter Antioxidants Application Note Food Testing & Agriculture Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd. Abstract

More information

Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector

Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector application Note UHPLC Author Njies Pedjie PerkinElmer, Inc. Shelton, CT 06484 USA Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector Introduction Foods

More information

NEW Kinetex F5. HPLC/UHPLC Core-Shell Columns

NEW Kinetex F5. HPLC/UHPLC Core-Shell Columns Core-Shell Technology NEW Kinetex Whoa! I can even separate structural isomers! TMS TMS HPLC/UHPLC Core-Shell Columns Reduce Method Development Time by Days Greater Reproducibility than other PPs Glorious

More information

Alcohol Biomarkers by UHPLC-MS/MS

Alcohol Biomarkers by UHPLC-MS/MS Alcohol Biomarkers by UHPLC-MS/MS Application #AN9 ACE Excel. C8 x. mm EXL--U A: mm ammonium fluoride B: MeCN....... ml/ µl C AB SCIEX triple quad Negative ESI MRM Source temperature: C IonSpray voltage:

More information

Vitamin Analysis by HPLC

Vitamin Analysis by HPLC Vitamin Analysis by HPLC Technical Note Vitamins are trace-amount organic compounds that regulate physiological functions of an organ- COSMOSIL 5C18 Fat-soluble Vitamins (1) Fat-soluble Vitamins Standard

More information

ZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006)

ZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006) September 2006 RESTRICTED ZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006) This document was provided by a contracted quality control laboratory.

More information

Sepax Technologies, Inc.

Sepax Technologies, Inc. Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid

More information

Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE. PLD+ Prior to LC-MS/MS Analysis

Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE. PLD+ Prior to LC-MS/MS Analysis Application Note AN842.V. Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE PLD+ Prior to LC-MS/MS Analysis Page Extraction of 25-hydroxy Vitamin D from Serum Using ISOLUTE PLD+ Prior to LC-MS/MS

More information

Transfer and Speed-up of Methods to Fused-Core Particle Columns EPA Method 1694

Transfer and Speed-up of Methods to Fused-Core Particle Columns EPA Method 1694 Transfer and Speed-up of Methods to Fused-Core Particle Columns EPA Method 1694 Ed Mauney, Hillel K. Brandes, William H. Campbell, Wayne K. Way, Richard A. Henry, David S. Bell, and Carmen T. Santasania

More information

SEDEX 90LT Presentation

SEDEX 90LT Presentation SEDEX 90LT Presentation HPLC 2011, Budapest SENSITIVITY FLEXIBILITY SEDERE EXPERIENCE SEDEX 90LT Technology SEDEX 990LT 0LT Light Source Selected Blue-Violet Laser 405nm 10mW Output SEDEX 90LT Technology

More information

A Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer

A Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer A Robustness Study for the Agilent 7 LC-MS/MS Mass Spectrometer Application Note Clinical Research Authors Linda Côté, Siji Joseph, Sreelakshmy Menon, and Kevin McCann Agilent Technologies, Inc. Abstract

More information

Chiral Screening for SFC and UHPLC with the Agilent 1260 Infinity II SFC/UHPLC Hybrid System

Chiral Screening for SFC and UHPLC with the Agilent 1260 Infinity II SFC/UHPLC Hybrid System Application Note Small Molecule Pharmaceutical Chiral Screening for SFC and UHPLC with the Agilent 16 Infinity II SFC/UHPLC Hybrid System Author Edgar Naegele, Agilent Technologies, Inc. Waldbronn, Germany

More information

SUPPORTING INFORMATION:

SUPPORTING INFORMATION: 1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld

More information

High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System

High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System Application Note Food, Hydrocarbon Processing Authors Michael Woodman Agilent Technologies,

More information

Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C18 Bonded Column

Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C18 Bonded Column Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C8 Bonded Column Application Note Food Authors Rongjie FU Agilent Technologies (Shanghai) Co. Ltd. Ying Lun Road Waigaoqiao Free

More information

Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column

Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column APPLICATION NOTE 21673 Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column Authors Aaron Lamb and Brian King, Thermo Fisher Scientific, Runcorn, UK

More information

HPLC Analysis with Fluorescence Detection of Chlorophyll Degradation Products Pheophytins and Pyropheophytin in Virgin Olive Oil

HPLC Analysis with Fluorescence Detection of Chlorophyll Degradation Products Pheophytins and Pyropheophytin in Virgin Olive Oil HPLC Analysis with Fluorescence Detection of Chlorophyll Degradation Products Pheophytins and Pyropheophytin in Virgin Olive Oil Application Note Authors Xueqi Li, Selina Wang UC Davis Olive Center Robert

More information

NEW. Kinetex. Biphenyl TMS TMS TMS TMS. Selectivity that a C18 just can t give you!

NEW. Kinetex. Biphenyl TMS TMS TMS TMS. Selectivity that a C18 just can t give you! Core-Shell Technology NEW Kinetex enyl Biph Biphenyl TM S TM Selectivity that a C8 just can t give you! S Bip he ylhenyl Bnip Remarkable Separation Power Rugged and Reliable % Aqueous Stable Kinetex Core-Shell

More information

GlycanPac AXR-1 Columns

GlycanPac AXR-1 Columns CHRMATGRAPHY GlycanPac AXR- Columns For High Resolution Glycan Analysis Product Specifications The Thermo Scientific GlycanPac AXR- columns are highperformance, silica-based HPLC columns for simultaneous

More information