Part III => METABOLISM and ENERGY. 3.6 Krebs Cycle 3.6a Pyruvate Oxidation 3.6b Krebs Cycle Reactions 3.6c Krebs Cycle Regulation

Size: px

Start display at page:

Download "Part III => METABOLISM and ENERGY. 3.6 Krebs Cycle 3.6a Pyruvate Oxidation 3.6b Krebs Cycle Reactions 3.6c Krebs Cycle Regulation"

Dwain Johns
6 years ago
Views:

1 Part III => METABOLISM and ENERGY 3.6 Krebs Cycle 3.6a Pyruvate Oxidation 3.6b Krebs Cycle Reactions 3.6c Krebs Cycle Regulation

2 Sources of Acetyl Group of Acetyl-CoA - Acetyl group of acetyl-coa can be derived from the breakdown of: (1) Carbs (glucose) 3.2 (2) Lipids (fatty acids) 3.4 (3) Proteins (amino acids) Under normal physiological conditions, the acetyl group of acetyl-coa is largely derived from pyruvate (the end product of glycolysis) see Pyruvate is transported into the mitochondrial matrix by the pyruvate-proton symporter located within the highly selective inner mitochondrial membrane (IMM) the relatively-porous outer mitochondrial membrane (OMM) largely harbors non-selective channels such as voltage-dependent anion channels and porins, which enable facilitated diffusion of most metabolites into the intermembrane space (IMS) - Within the mitochondrial matrix, oxidation of acetyl group of acetyl-coa to CO 2 via Krebs cycle generates free energy that is captured in the form of reduced NADH/FADH 2 and high-energy GTP

3 Section 3.6a: Pyruvate Oxidation

4 Synopsis 3.6a - A major source of acetyl group of acetyl-coa entering the Krebs cycle is derived from pyruvate generated from carbohydrates via glycolysis - Transfer of the acetyl group of pyruvate to acetyl-coa occurs via oxidative decarboxylation involving a five-step reaction catalyzed by a multi-enzyme system collectively referred to as pyruvate dehydrogenase complex or PDC - Each of the five steps catalyzed by PDC, occurring within the mitochondrial matrix, requires a specific coenzyme: (1) Thiamine pyrophosphate (TPP) (2) Lipoamide/Lipoic acid (LPA) (3) Coenzyme A (CoA) (4) Flavin adenine dinucleotide (FAD) (5) Nicotinamide adenine dinucleotide (NAD + ) - Synthesis of acetyl-coa from pyruvate can be considered as a preparatory stage, or Step 0 of the Krebs cycle - Overall reaction (irreversible) catalyzed by PDC is: Pyruvate + CoA + NAD + ==> Acetyl-CoA + CO 2 + NADH

PDC Catalysis: Multienzyme System - Pyruvate dehydrogenase complex (PDC) is a multi-enzyme system comprised of three distinct subenzymes: (a) Pyruvate dehydrogenase (E1) (b) Dihydrolipoamide

5 PDC Catalysis: Multienzyme System - Pyruvate dehydrogenase complex (PDC) is a multi-enzyme system comprised of three distinct subenzymes: (a) Pyruvate dehydrogenase (E1) (b) Dihydrolipoamide transacetylase (E2) (c) Dihydrolipoamide dehydrogenase (E3) - PDC catalyzes the overall reaction: Pyruvate + CoA + NAD + Acetyl-CoA + CO 2 + NADH - This reaction can be subdivided into five distinct catalytic steps, each mediated by a specific subenzyme-coenzyme system, as outlined below: (1) E1.TPP (2) E2-LPA (3) E2.CoA (4) E3.FAD (5) E3.NAD +

6 PDC Catalysis: (Dihydro)lipoamide Reduction Lipoamide Oxidation Dihydrolipoamide Lipoic Acid Lipoic acid is covalently attached via an amide linkage to a lysine residue in E2 dihydrolipoamide transacetylase hence often termed lipoamide Specific roles of the three subenzymes in PDC are: (1) Pyruvate dehydrogenase (E1) oxidizes/decarboxylates pyruvate, transferring the acetyl group to bound TPP cofactor (2) Dihydrolipoamide/dihydrolipoyl transacetylase (E2) transfers the acetyl group from TPP in E1 to bound lipoamide cofactor, and then from lipoamide to CoA resulting in the reduction of lipoamide to dihydrolipoamide (3) Dihydrolipoamide/dihydrolipoyl dehydrogenase (E3) oxidizes dihydrolipoamide to lipoamide in E2 using FAD and NAD + as oxidizing agents

PDC Catalysis: Catalytic Steps LPA Cys-Cys disulfide bridge 1 E1 prosthetic group (noncovalently bound) 2 3 4 5

7 PDC Catalysis: Catalytic Steps LPA Cys-Cys disulfide bridge 1 E1 prosthetic group (noncovalently bound) Lipoamide (LPA) E2 prosthetic group (covalently bound) E2 cosubstrate E3 prosthetic group (noncovalently bound) E3 cosubstrate

ring and pyrophosphate - E1 pyruvate dehydrogenase (which harbors TPP cofactor) decarboxylates pyruvate resulting in the formation of hydroxyethyl-tpp.

8 PDC Catalysis: (1) E1.TPP Thiamine Pyrophosphate (TPP) Pyrimidine Thiazole Pyrophosphate TPP consists of a central thiazole ring harboring a carbanion flanked between a pyrimidine ring and pyrophosphate - E1 pyruvate dehydrogenase (which harbors TPP cofactor) decarboxylates pyruvate resulting in the formation of hydroxyethyl-tpp.e1 carbanion intermediate - The reaction is mediated by the nucleophilic attack of TPP carbanion on the carbonyl atom of the acetyl moiety on pyruvate - Recall that TPP is also required for the decarboxylation of pyruvate to acetaldehyde by pyruvate decarboxylase during alcoholic fermentation (see 3.2c)

9 PDC Catalysis: (2) E2-LPA Hydroxyethyl-TPP.E1 Lipoamide (LPA) Lipoamide consists of lipoic acid covalently linked via an amide bond to the -amino group of a lysine residue in E2 Hydroxyethyl moiety of hydroxyethyl-tpp.e1 intermediate is transferred to E2 dihydrolipoamide transacetylase harboring a lipoamide cofactor (lipoamide-e2) via two steps: (1) Hydroxyethyl carbanion in the hydroxyethyl-tpp.e1 complex launches nucleophilic attack on the lipoamide disulfide in lipoamide-e2 (2) Hydroxyethyl carbanion is subsequently oxidized to an acetyl group with concomitant reduction of lipoamide resulting in the formation of acetyl-dihydrolipoamide-e2 intermediate and regeneration of active TPP.E1

10 - The subsequent reactions merely act to regenerate active lipoamide-e2 complex Reactive thiol group PDC Catalysis: (3) E2.CoA Coenzyme A (CoA) - E2 dihydrolipoamide transacetylase catalyzes the transfer of acetyl group of acetyl-dihydrolipoamide-e2 intermediate to CoA - This results in the formation of acetyl-coa (the Krebs cycle substrate) with concomitant release of dihydrolipoamide-e2 intermediate

11 PDC Catalysis: (4) E3.FAD Cys-Cys disulfide bridge E3(ox).FAD Dihydrolipoamide-E2 E3(red).FAD Lipoamide-E2 - Oxidized E3 dihydrolipoamide dehydrogenase [E3(ox).FAD] oxidizes the dihydrolipoamide-e2 intermediate to active lipoamide-e2 complex in a disulfide exchange reaction - In so doing, the disulfide linkage of E3 becomes reduced to thiol groups resulting in the conversion of E3(ox).FAD complex to E3(red).FAD intermediate

12 PDC Catalysis: (5) E3.NAD + E3(red).FAD E3(ox).FADH 2 E3(ox).FAD - FAD oxidizes the thiol groups of reduced E3 dihydrolipoamide dehydrogenase [E3(red).FAD] intermediate resulting in the formation of E3(ox).FADH 2 intermediate - FADH 2 within the E3(ox).FADH 2 intermediate is subsequently oxidized back to FAD by funneling its electrons to NAD + in an electron exchange reaction, thereby yielding an active E3(ox).FAD complex with concomitant release of NADH - Recall that FAD is a more powerful oxidizing agent than NAD + ( FAD > NAD+ ) thus, it is energetically unfavorable for electrons to flow from FADH 2 to NAD + however, E3 overrides such energetic penalty by bringing NAD + and FAD within close proximity to each other so as to allow the latter to serve as an electron conduit rather than an electron sink NAD +

13 Exercise 3.6a - Write an equation for the pyruvate dehydrogenase complex (PDC) reaction - Describe the five reactions of the pyruvate dehydrogenase complex (PDC) - What cofactors are required? Which of these are prosthetic groups?

14 Section 3.6b: Krebs Cycle Reactions

15 Synopsis 3.6b - Krebs cycle involves the oxidation of acetyl group of acetyl-coenzyme A (acetyl-coa) to CO 2 with concomitant release of NADH, FADH 2, and GTP - Such oxidation of acetyl groups occurs via a cycle rather than a pathway since both the substrate and the product are identical (oxaloacetate), or simply put, the substrate ultimately cycles to itself in a series of reactions this is in contrast to a pathway in which a substrate undergoes conversion to a chemically-distinct product! - Krebs cycle is comprised of a total of eight enzymatic steps excluding Step 0 for the synthesis of acetyl-coa and occurs within the mitochondrial matrix - Overall reaction scheme is: Acetyl-CoA + 3NAD + + FAD + GDP + P i <=> CoA + 3NADH + FADH 2 + GTP + 2CO 2 - In some bacteria, Krebs cycle runs counterclockwise in order to generate C compounds such as acetyl-coa solely from CO 2 and H 2 O in this so-called reverse Krebs cycle, a subset of alternative enzymes are employed (like pathways, cycles are not wholly reversible!) - In addition to Krebs cycle, other notable metabolic cycles include: Cori cycle: lactate glucose Cahill cycle: alanine glucose Calvin cycle: CO 2 C compounds (C fixation plants only!)

Krebs Cycle: Overview - Widely considered as the metabolic hub due to the fact that a major portion of macronutrients such as carbs, fats, and proteins are oxidized via Krebs cycle in

16 Krebs Cycle: Overview - Widely considered as the metabolic hub due to the fact that a major portion of macronutrients such as carbs, fats, and proteins are oxidized via Krebs cycle in order to generate free energy and other metabolites needed to sustain life - Named after Krebs, it is also known as the citric acid cycle and tricarboxylic acid cycle Hans Krebs ( ) GDP

Mono- and Dicarboxylic Acids Monocarboxylate Anions - - -

Propionate (Propanoate) 4:1 Butyrate (Butanoate) 5:1

- - 2:2 Oxalate (Ethanedioate) 3:2 Malonate

17 Mono- and Dicarboxylic Acids Monocarboxylate Anions :1 Formate (Methanoate) 2:1 Acetate (Ethanoate) 3:1 Propionate (Propanoate) 4:1 Butyrate (Butanoate) 5:1 Valerate (Pentanoate) Dicarboxylate Dianions :2 Oxalate (Ethanedioate) 3:2 Malonate (Propanedioate) 4:2 Succinate (Butanedioate) 5:2 Glutarate (Pentanedioate)

18 Krebs Cycle: A Logical Approach -Ketopropionate -Ketosuccinate (Oxaloacetate) -Hydroxy- -carboxyglutarate (Citrate) L- -Hydroxysuccinate (L-Malate) D- -Hydroxy- -carboxyglutarate (D-Isocitrate) Didehydrosuccinate (Fumarate) -Ketoglutarate Succinate Succinyl-CoA

19 Krebs Cycle Reactions: (1) Citrate Synthase Catalyzes the condensation of acetyl-coa and oxaloacetate ( -ketosuccinate) to produce citrate ( -hydroxy- -carboxyglutarate) and CoA: (a) Acid-base catalysis mediated by active site D375/H274 residues produces a highly reactive acetyl-coa enolate nucleophile from acetyl-coa and oxaloacetate (b) Subsequent nucleophilic attack of acetyl-coa enolate on the carbonyl group of oxaloacetate generates citryl-coa intermediate (c) The citryl-coa intermediate spontaneously hydrolyzes into citrate and free CoA

20 Krebs Cycle Reactions: (2) Aconitase Catalyzes the isomerization of citrate ( -hydroxy- -carboxyglutarate) to isocitrate ( hydroxy- -carboxyglutarate): (a) Dehydration of citrate resulting in the elimination of a H 2 O molecule to generate cis-aconitate intermediate with a C=C double bond (b) Hydration of the C=C double bond in cis-aconitate intermediate generates isocitrate at the expense of a H 2 O molecule Is aconitase a mutase (since it overall mediates intramolecular transfer of a group)? But, aconitase also mediates bond cleavage to generate cis-aconitate intermediate? Mechanistically, aconitase is classified as a lyase rather than a mutase!

21 Krebs Cycle Reactions: (3) Isocitrate Dehydrogenase Catalyzes the oxidative decarboxylation of isocitrate ( -hydroxy- -carboxyglutarate) to -ketoglutarate : (a) Oxidation of isocitrate (using NAD + as oxidizing agent) to oxalosuccinate intermediate harboring a newly formed carbonyl group with concomitant release of NADH (b) Hyperpolarization of newly formed carbonyl group in oxalosuccinate by Mn 2+ ion facilitates the release of a CO 2 molecule resulting in the formation of a transient enolate intermediate subsequent protonation of which generates -ketoglutarate

22 Krebs Cycle Reactions: (4) -Ketoglutarate Dehydrogenase Catalyzes the oxidative decarboxylation of -ketoglutarate to succinyl-coa: (a) Oxidation of -ketoglutarate (using NAD + as oxidizing agent) facilitates the release of a CO 2 molecule (b) Transfer of the thiol group of CoA-SH generates high-energy succinyl-coa recall that thioester bonds (like phosphoanhydride bonds) are high-energy (their hydrolysis releases lots of free energy to drive endergonic reactions)!

23 Krebs Cycle Reactions: (5) Succinyl-CoA Synthetase Catalyzes the cleavage of high-energy succinyl-coa to succinate coupled with the synthesis of high-energy GTP via three steps: (1) Condensation of P i and succinyl-coa to generate succinyl-phosphate (SucP) and CoA (2) Transfer of phosphoryl group of SucP to a histidine on the enzyme so as to release succinate (3) Transfer of phosphoryl group from the histidine on the enzyme to GDP to generate GTP

24 Krebs Cycle Reactions: (6) Succinate Dehydrogenase Catalyzes dehydrogenation of succinate to fumarate (didehydrosuccinate) using FAD (covalently bound to the enzyme via a histidine residue) as an oxidizing agent (more powerful than NAD + ): (a) Electrons from succinate are funneled to FAD resulting in the formation of a C=C double bond in fumarate (b) Enzyme-bound FAD is reduced to FADH 2

25 Krebs Cycle Reactions: (7) Fumarase Catalyzes hydration of C=C double bond in fumarate (didehydrosuccinate) to malate ( -hydroxysuccinate): (a) Nucleophilic attack of an hydroxyl anion (from H 2 O) on C=C double bond in fumarate generates a carbanion intermediate (b) Protonation of carbanion intermediate generates malate Fumarase belongs to the hydratase family of enzymes addition of H 2 O to unsaturated bonds such as C=C

26 Krebs Cycle Reactions: (8) Malate Dehydrogenase Catalyzes oxidation of malate ( -hydroxysuccinate) to oxaloacetate ( -ketosuccinate): (a) Electrons in the form of an hydride ion are funneled to NAD + so as to oxidize the hydroxyl moiety in malate to a keto group in oxaloacetate (b) NAD + is reduced to NADH

27 Exercise 3.6b - Explain why the citric acid cycle is considered to be the hub of cellular metabolism - What are the substrates and products of the net reaction corresponding to one turn of the citric acid cycle? - Draw the structures of the eight intermediates of the citric acid cycle and name the enzymes that catalyze their interconversions - Which steps of the citric acid cycle release CO 2 as a product? - Which steps produce NADH or FADH 2? Which step produces GTP?

28 Section 3.6c: Krebs cycle Regulation

29 Synopsis 3.6c - Given that Krebs cycle plays a central role in the oxidation of macronutrients to generate energy, it is imperative that it be tightly regulated depending on cellular demands - Key enzymes involved in Krebs cycle regulation are: (a) Pyruvate dehydrogenase (b) Citrate synthase (c) Isocitrate dehydrogenase (d) -Ketoglutarate dehydrogenase - Regulatory mechanisms include: - Feedback inhibition - Feedforth activation - Allosteric regulation - Post-translational modification (PTM)

Krebs cycle Products - Oxidation of acetyl group of acetyl- CoA results in the liberation of free energy and electrons - Free energy is conserved in the form of GTP which can be readily converted to

30 Krebs cycle Products - Oxidation of acetyl group of acetyl- CoA results in the liberation of free energy and electrons - Free energy is conserved in the form of GTP which can be readily converted to ATP via the action of NDK (nucleoside diphosphate kinase): ADP + GTP < = > ATP + GDP - Electrons are stored in the form of NADH and FADH 2 which will be ultimately funneled into the electron transport chain (ETC) to reduce O 2 to H 2 O to generate ATP via oxidative phosphorylation ( 3.6)

31 Krebs cycle Thermodynamics (in cardiomyocytes) Step Enzyme G / kj.mol -1 G / kj.mol -1 0 Pyruvate dehydrogenase << 0 << 0 1 Citrate synthase -32 << 0 2 Aconitase Isocitrate dehydrogenase -21 << 0 4 -Ketoglutarate dehydrogenase -33 << 0 5 Succinyl-CoA synthetase Succinate dehydrogenase Fumarase Malate dehydrogenase Recall that G = G + RT lnk eq ( 1.1) where G is the actual free energy change under non-equilibrium (steady-state) conditions, and G is the standard free energy equilibrium! - Since living cells operate under steady-state rather than equilibrium setting, the free energy changes associated with various Krebs cycle steps are largely concerned with G - Of the nine steps of Krebs cycle, only four (Steps 0, 1, 3 and 4) operate far from equilibrium ( G << 0) implying that they are primarily responsible for flux control ie they are the rate-determining steps of Krebs cycle!

32 Krebs cycle Regulation: Key Enzymes 0 a Key enzymes involved in Krebs cycle regulation are: (a) Pyruvate dehydrogenase (Step 0) (b) Citrate synthase (Step 1) (c) Isocitrate dehydrogenase (Step 3) (d) -Ketoglutarate dehydrogenase (Step 4) 1 b c 3 Activator Inhibitor Point of Inhibition d 4

Krebs cycle Regulation: (a) Pyruvate Dehydrogenase Acetyl-CoA NADH E1-OH (active) Pyruvate NAD + PDK PDP - Pyruvate dehydrogenase (E1) is a component of pyruvate dehydrogenase complex (PDC)

Ca 2+ (1) Feedback Inhibition PDC reaction products acetyl-coa and NADH respectively compete with corresponding substrates CoA and NAD + for the E1 active site, thereby slowing down the enzyme as the

33 Krebs cycle Regulation: (a) Pyruvate Dehydrogenase Acetyl-CoA NADH E1-OH (active) Pyruvate NAD + PDK PDP - Pyruvate dehydrogenase (E1) is a component of pyruvate dehydrogenase complex (PDC) multi-enzyme system, which catalyzes the overall reaction: Pyruvate + CoA + NAD + => Acetyl-CoA + CO 2 + NADH - E1 is under tight regulation via three major mechanisms: E1-OPO 3 2- (inactive) Insulin Ca 2+ (1) Feedback Inhibition PDC reaction products acetyl-coa and NADH respectively compete with corresponding substrates CoA and NAD + for the E1 active site, thereby slowing down the enzyme as the products accumulate (2) Post-Translational Modification (PTM) - PDC reaction products acetyl-coa and NADH activate pyruvate dehydrogenase kinase (PDK) which in turn phosphorylates a serine residue in E1 resulting in its inactivation - Mitogenic signals (demanding energy production) such as insulin and Ca 2+ reverse this inactivation by virtue of their ability to activate pyruvate dehydrogenase phosphatase (PDP) which in turn dephosphorylates E1, thereby promoting its activation (3) Feedforth Activation Accumulation of pyruvate and NAD + substrates serve as a signal for the inhibition of PDK, thereby favoring the activation of E1 and driving the PDC reaction forward in the direction of acetyl-coa synthesis

34 Krebs cycle Regulation: (b) Citrate Synthase Citrate Succinyl-CoA NADH Oxaloacetate + Acetyl-CoA Citrate synthase Citrate + CoA Citrate synthase is regulated by: Feedback Inhibition inhibited directly by its own product citrate as well as downstream products succinyl-coa and NADH

Krebs cycle Regulation: (c) Isocitrate Dehydrogenase ADP Ca 2+ NADH ATP Isocitrate + NAD + Isocitrate dehydrogenase -Ketoglutarate + CO 2 + NADH Isocitrate dehydrogenase is regulated by: (1) Feedback

35 Krebs cycle Regulation: (c) Isocitrate Dehydrogenase ADP Ca 2+ NADH ATP Isocitrate + NAD + Isocitrate dehydrogenase -Ketoglutarate + CO 2 + NADH Isocitrate dehydrogenase is regulated by: (1) Feedback Inhibition inhibited directly by its own product NADH, which competes with NAD + for binding to the active site (2) Allosteric Modulation while ATP allosterically inhibits the enzyme, ADP exerts exactly the opposite effect binding of Ca 2+ also augments its catalytic activity

Krebs cycle Regulation: (d) -Ketoglutarate Dehydrogenase Ca 2+ Succinyl-CoA NADH -Ketoglutarate + CoA + NAD + -Ketoglutarate dehydrogenase Succinyl-CoA + CO 2 + NADH -Ketoglutarate dehydrogenase is

36 Krebs cycle Regulation: (d) -Ketoglutarate Dehydrogenase Ca 2+ Succinyl-CoA NADH -Ketoglutarate + CoA + NAD + -Ketoglutarate dehydrogenase Succinyl-CoA + CO 2 + NADH -Ketoglutarate dehydrogenase is regulated by: (1) Feedback Inhibition inhibited directly by its own products succinyl-coa and NADH, which respectively compete with -ketoglutarate and NAD + substrates for binding to the active site (2) Allosteric Modulation Ca 2+ binds and activates the enzyme though the extent to which this represents an allosteric effect is debatable

37 Exercise 3.6c - How much ATP can be generated from glucose when the citric acid cycle is operating? - Which steps of the citric acid cycle regulate flux through the cycle? - Describe the role of ADP, Ca 2+, acetyl-coa, and NADH in regulating pyruvate dehydrogenase and the citric acid cycle.

Citric Acid Cycle: Central Role in Catabolism. Entry of Pyruvate into the TCA cycle

Citric Acid Cycle: Central Role in Catabolism Stage II of catabolism involves the conversion of carbohydrates, fats and aminoacids into acetylcoa In aerobic organisms, citric acid cycle makes up the final