LC-MS. Pre-processing (xcms) W4M Core Team. 29/05/2017 v 1.0.0

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "LC-MS. Pre-processing (xcms) W4M Core Team. 29/05/2017 v 1.0.0"

Transcription

1 LC-MS Pre-processing (xcms) W4M Core Team 29/05/2017 v 1.0.0

2 Acquisition files upload and pre-processing with xcms: extraction, alignment and retention time drift correction. SECTION 1 2

3 LC-MS Data What is provided by the mass spectrometers What we want for data analysis

4 Extraction with XCMS R based software, Free A lot of parameters to tune, No graphical interface Need to write a R script Web for documentation: Forums :

5 Extraction with XCMS Extraction Extraction of ions in each sample independantly. Grouping alignment Each ion is aligned across all samples Retention time correction (optional) Fill peaks Replace missing data with baseline value CAMERA Annotation Statistics and visualisation (optionals) CAMERA For annotation of adducts, neutral loss and isotopes

6 Extraction with XCMS CAMERA Annotation of Adduct Fragments and isotopes

7 DATA 7

8 Data Raw data: mzxml, mzml, mzdata and netcdf samplemetadata HU_neg_011_b2 HU_neg_014_b2 Blank04 Blank05 bio bio blank blank Add some informations for further steps 8

9 Data Raw data: mzxml, mzml, mzdata and netcdf samplemetadata samples class sampletype subset full injectionorder batch age HU_neg_011_b2 bio sample ne1 19 HU_neg_014_b2 bio sample ne2 22 Blank04 blank blank ne2 NA Blank05 blank blank ne1 NA Add some informations for further steps 9

10 Two strategies 10

11 Two strategies 11 The "old" system -> files are nested in folders for their groups within a zip file + The folders set the group of the files for xcms.group + Only one import and one step - xcmsset is limited to 6 CPUs - The files aren't integrated into the history and can't be visualized (one day)

12 Two strategies The "brand new" system -> files are uploaded individually and processed in parallel - The xcmsset outputs have to be merged before using group - A samplemetadata file must be used to set the group (but you need one for some further steps anyway) + One xcmsset job is launch for each input file. It is highly parallelizable + The files are completely integrated in Galaxy and can be one day vizualized + A better transparency 12

13 Dataset Collection Dataset collection allow to group N datasets in 1 wrap / collection A Dataset collection depending of the tool will process nested datasets In one step tool In parallel xcmsset xcmsset xcmsset 13

14 Dataset Collection 14

15 Dataset Collection 15

16 Dataset Collection 1 16

17 Dataset collection XCMSSET 17

18 Dataset Collection Dataset collection allow to group N datasets in 1 wrap / collection A Dataset collection depending of the tool will process nested datasets In one step tool In parallel xcmsset xcmsset xcmsset 18

19 Dataset Collection 19

20 Dataset Collection 20

21 Dataset Collection 1 21

22 RUN! XCMSSET 22

23 mzxml raw file mzxml in a text editor 1 scan 23

24 mzxml raw file informations Real life example : m/z 187 fichier HU_neg_091.mzXML scan # RetTime (sec) basepeakmz int TIC %TIC delta ppm delta dalton E E+07 42% E E+07 62% E E+07 65% E E+07 67% E E+08 68% E E+08 68% E E+08 69% E E+08 67% E E+07 66% E E+07 65% E E+07 65% E E+07 63% E E+07 62% E E+07 58% E E+07 50% E E+07 39% E E+07 35% E E+07 29% m/z deviation RT range m/z median Scan to scan m/z deviation

25 mzxml raw file informations Real life example : m/z 187 fichier HU_neg_091.mzXML scan # RetTime (sec) basepeakmz int TIC %TIC delta ppm delta dalton E E+07 42% E E+07 62% E E+07 65% E E+07 67% E E+08 68% E E+08 68% E E+08 69% E E+08 67% E E+07 66% E E+07 65% E E+07 65% E E+07 63% E E+07 62% E E+07 58% E E+07 50% E E+07 39% E E+07 35% E E E+07 29% E+07 RT range m/z median E E E E E E E+07 peak width = E

26 xcms Extraction centwave algorithm The algorithm aims detecting «Mass traces» or «region of interest» (ROI) which are defined as regions with less than a defined deviation of m/z in consecutive scans. This deviation must be lower than the value of the parameter «ppm» The value (unit ppm) has to be set according to mass spectrometer accuracy. ROI mass intensities are then used to define the chomatographic peak with continuous wavelet transform algorithm. peakwidth (min, max) has to be set for this step. 20,50 for HPLC 5,12 for UPLC Tautenhahn R. BMC Bioinformtics 2008

27 xcms Extraction algorithms MatchedFilter is dedicated to centroid or profile low resolution MS data Centwave is dedicated to centroid high resolution MS data 27

28 xcms Extraction centwave algorithm Centwave chromatographic peaks detection. Tautenhahn R., BioInformatics, 2008

29 xcms Extraction centwave algorithm Centwave chromatographic peaks detection. Tautenhahn R., BioInformatics, 2008

30 centwave basic parameters CAMERA Annotation of Adduct Fragments and isotopes

31 centwave noise and coeluted peaks CAMERA Annotation of Adduct Fragments and isotopes

32 xcms centwave parameters xcms forum : How to choose peakwidth? "The main purpose of the peakwidth parameter is to roughly estimate the peak width range, this parameter is not a threshold. The wavelets used for peak detection are calculated from this parameter. If you use HPLC and your peaks are normally s wide (base peak with), just go with that, i.e. peakwidth=c(20,60) centwave will still detect peaks that are 15s or 80 s wide! Important: Do not choose the minimum peak width too small, it will not increase sensitivity, but cause peaks to be split." Example: peak width ~ 45 s Using peakwidth = c(20,60) the peak will be split in three peaks, each detected as a ~10s wide separate peak (since they are separated by a local minimum) : using peakwidth = c(20,120) will keep the peak intact :

33 xcms extraction output CAMERA Annotation of Adduct Fragments and isotopes

34 xcms extraction output CAMERA Annotation of Adduct Fragments and isotopes When a zip file of is used a samplemetadata.tsv is created at this step. It must contains all informations needed for further analyses: batch correction and statistical analyses. This file must be downloaded in order to add all these informations and then uploaded.

35 xcms extraction output CAMERA Annotation of Adduct Fragments and isotopes

36 extraction parameters summary xcms steps xcms parameters related to description examples Extraction ppm m/z fluctuation of m/z value (ppm) from scan to scan. 5 (xcmsset) Depends on the mass spectrometer resolution peakwidth retention time range of chromatographic peak width (second) UPLC 5,20 HPLC 10,40 mzdiff m/z and retention time Minimum difference of mz for peaks with overlapping retention time (coeluting peak). Must be negative to allow overlap or 0.05 Prefilter Intensity A peak must be present in n scans with an intensity n=3,k=1000 greater than k. snthresh Intensity Ratio signal/noise threshold 3 noise Intensity Each centroid must be greater than "noise" value.

37 xcmsset 37

38 «Grouping» step Independant peaklists pool1b1 pool1b2 pool1b3 mz rt int mz rt int mz rt int Group ions by m/z Group by retention time mz rt int mz rt int mz rt int mz rt int mz rt int mz rt int Resulting matrix mz rt pool1b1 pool1b2 pool1b

39 xcms alignment group First step, a binning of mass domain is performed. The size of the bin is defined by mzwid parameter. Then for each mz bin, all ions of all samples are taken into account for all retention times. Kernel density estimator method is used to detect region of retention time with high density of ions. mzwid

40 xcms alignment group A gaussian model group together peaks with simillar retention time. The inclusivness of ions in a group is defined by the the standard deviation of the gaussian model (bandwith) corresponding to of the bw parameter xcms. This parameter can be interpreted as a retention time window. Vertical dash lines indicates that the feature is valid and will be retain in the data Matrix To be valid, the number of peaks in a group must be greater than the a percentage of the total number of samples. This threshold is defined by the minfrac parameter. mzwid Problem bw = 30 sec

41 xcms alignment group mzwid bw = 30 sec Problem Solved bw = 10 sec Decreasing bw allows to separate these 2 groups. The resulting m/z and retention time of the feature correspond to the median of m/z and RT of all ions grouped together as a single feature.

42 Minfrac parameter for group Minfrac = samples in each group m/z Minfrac = minimum sample detected in at least one class to be considered as a group RT m/z RT 42

43 Minfrac parameter for group Minfrac = samples in each group m/z RT

44 group interface CAMERA Annotation of Adduct Fragments and isotopes

45 xcms group output mzwid define the intervals of m/z CAMERA Annotation of Adduct Fragments and isotopes bw define the width of the gaussian curve

46 xcms group output Two distinct m/z merge as one group. Mzwid and bw too large

47 xcms group output Two distinct m/z are separated by decreasing bandwith value.

48 grouping parameters summary xcms steps Alignment (group) xcms parameters related to definition examples mzwid m/z Size of mz slices (bins). Range of m/z to be included in a group. Depends on mass spectrometer accuracy. bw retention standart deviation of the gaussian metapeak that group time together peaks minfrac samples A group to be valid must be found in minfrac*total n=10, number of samples in each subfolder of datafiles. minfrac=0.5 minfrac=0.5 correspond to 50%. found in at least 5 max number of ions Maximum number of groups detected in a single mz slices. 10 or 50

49 xcms workflow retcor CAMERA Annotation of Adduct Fragments and isotopes

50 xcms retcor output CAMERA Annotation of Adduct Fragments and isotopes retcor improving retention time must be followed by a second group step.

51 xcms retcor output Modification of the degre of smoothing Span = 0.8 CAMERA Annotation of Adduct Fragments and isotopes

52 Parameters for retcor Missing = 1 4 samples in each group m/z RT Extra = 1 m/z RT 52

53 xcms retcor obiwarp 53

54 retcor parameters summary xcms steps Retention time correction (retcor) xcms parameters smooth method related to description examples retention time Regression model to model time deviation among samples (linear or loess) linear or loess span degree of smoothing of the loess model. 0.2 to 1 extra samples number of "extra" peaks use to define reference peaks default=1 (or well behaved peaks) for modeling time deviation. Number of Peaks > number of samples. missing samples number of samples without reference peaks. If blank samples are used, missing = number of blanks. ploytype retention time Define the graphical visualistion of the effect of the model on retention time correction. number of blank samples deviation

55 Second grouping As retcor improved retention drift among samples a new grouping is mandatory to take advantage of this correction. bw parameter can thus be set to a smaller value than in the first group step. CAMERA Annotation of Adduct Fragments and isotopes

56 xcms fillpeaks Filling method: «chrom» for LCMS «MSW» for direct injection. CAMERA Annotation of Adduct Fragments and isotopes

57 MS data processing Report creation and Annotations Yann GUITTON 29/05/2017 v 1.0.0

SimLipid 3.5. Manual PREMIER. Biosoft International Corina Way, Palo Alto, CA Tel: (650) FAX: (650)

SimLipid 3.5. Manual PREMIER. Biosoft International Corina Way, Palo Alto, CA Tel: (650) FAX: (650) SimLipid 3.5 Manual PREMIER Biosoft International 3786 Corina Way, Palo Alto, CA 94303-4504 Tel: (650) 856-2703 FAX: (650) 843-1250 E-mail: sales@premierbiosoft.com Copyright 2003 by PREMIER Biosoft International.

More information

SimGlycan. A high-throughput glycan and glycopeptide data analysis tool for LC-, MALDI-, ESI- Mass Spectrometry workflows.

SimGlycan. A high-throughput glycan and glycopeptide data analysis tool for LC-, MALDI-, ESI- Mass Spectrometry workflows. PREMIER Biosoft SimGlycan A high-throughput glycan and glycopeptide data analysis tool for LC-, MALDI-, ESI- Mass Spectrometry workflows SimGlycan software processes and interprets the MS/MS and higher

More information

Thermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS

Thermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS Thermo Scientific LipidSearch Software for Lipidomics Workflows Automated Identification and Relative of Lipids by LC/MS The promise of lipidomics Lipidomics is a new field of study crucial for understanding

More information

A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.

A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin

More information

CEU MASS MEDIATOR USER'S MANUAL Version 2.0, 31 st July 2017

CEU MASS MEDIATOR USER'S MANUAL Version 2.0, 31 st July 2017 CEU MASS MEDIATOR USER'S MANUAL Version 2.0, 31 st July 2017 1. Introduction... 2 1.1. System Requirements... 2 2. Peak search... 3 2.1. Simple Search... 3 2.2. Advanced Search... 5 2.3. Batch Search...

More information

How to Use TOF and Q-TOF Mass Spectrometers

How to Use TOF and Q-TOF Mass Spectrometers How to Use TOF and Q-TOF Mass Spectrometers October 2011 What do TOF and Q-TOF offer? TOF Fast scanning of full spectrum High resolution full scan spectra Accurate mass measurements Q-TOF Fast scanning

More information

Lipid annotation with MS2Analyzer. Yan Ma 10/24/2013

Lipid annotation with MS2Analyzer. Yan Ma 10/24/2013 Lipid annotation with MS2Analyzer Yan Ma 10/24/2013 Checklist before you start You need to have: 1.A computer with Java environment and Office(2003 or higher) 2.MS/MS spectra in MGF files 3.Latest version

More information

Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS

Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS Comprehensive Forensic Toxicology Screening in Serum using On-Line SPE LC-MS/MS SCIEX QTRAP 4500 LC-MS/MS System and Spark Holland PICO Adrian M. Taylor 1, Peter Ringeling 2, Martin Sibum 2, Stefan Sturm

More information

Towards High Resolution MS in Regulated Bioanalysis

Towards High Resolution MS in Regulated Bioanalysis Towards High Resolution MS in Regulated Bioanalysis Benno Ingelse MSD 3 rd EB focus meeting June 12 th 13 th Brussels, Belgium Contributors Gary Adamson Ken Anderson Kevin Bateman Cynthia Chavez-Eng Inhou

More information

Chemical Analysis Business Operations Waters Corporation Milford MA

Chemical Analysis Business Operations Waters Corporation Milford MA The Detection and Identification of Unknown Contaminants During ToF Screening and Structural Elucidation for Pesticides in River Water Using an Integrated Software Approach Chemical Analysis Business Operations

More information

Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research

Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research Analysis of Testosterone, Androstenedione, and Dehydroepiandrosterone Sulfate in Serum for Clinical Research Dominic Foley, Michelle Wills, and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION

More information

SYNAPT G2-S High Definition MS (HDMS) System

SYNAPT G2-S High Definition MS (HDMS) System SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business

More information

Biological Mass Spectrometry. April 30, 2014

Biological Mass Spectrometry. April 30, 2014 Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing

More information

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS LC/MS/MS SOLUTIONS FOR LIPIDOMICS Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS Lipids play a key role in many biological processes, such as the formation of cell membranes and signaling

More information

Increased Identification Coverage and Throughput for Complex Lipidomes

Increased Identification Coverage and Throughput for Complex Lipidomes Increased Identification Coverage and Throughput for Complex Lipidomes Reiko Kiyonami, David Peake, Yingying Huang, Thermo Fisher Scientific, San Jose, CA USA Application Note 607 Key Words Q Exactive

More information

Phospholipid characterization by a TQ-MS data based identification scheme

Phospholipid characterization by a TQ-MS data based identification scheme P-CN1716E Phospholipid characterization by a TQ-MS data based identification scheme ASMS 2017 MP-406 Tsuyoshi Nakanishi 1, Masaki Yamada 1, Ningombam Sanjib Meitei 2, 3 1 Shimadzu Corporation, Kyoto, Japan,

More information

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics July.3.13 Ken Miller Vice President of Marketing, Life Sciences Mass Spectrometry 1 The world leader in serving science Omics & the

More information

Characterization of an Unknown Compound Using the LTQ Orbitrap

Characterization of an Unknown Compound Using the LTQ Orbitrap Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown

More information

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation

More information

Agilent Lipidomics. Workflow Guide. MS Data Qualitative Workflow. With MS/MS ID Confirmation. Find features in one file by MFE in MH Qual.

Agilent Lipidomics. Workflow Guide. MS Data Qualitative Workflow. With MS/MS ID Confirmation. Find features in one file by MFE in MH Qual. Agilent Lipidomics Workflow Guide A MS Data Lipidomics Workflows B MS/MS Data Lipidomics Workflows MS Data Qualitative Workflow MS Data Profiling Workflow With MS/MS ID Confirmation MS/MS Data Qualitative

More information

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS

More information

Data Preprocessing Method for Liquid Chromatography Mass Spectrometry Based Metabolomics

Data Preprocessing Method for Liquid Chromatography Mass Spectrometry Based Metabolomics pubs.acs.org/ac Data Preprocessing Method for Liquid Chromatography Mass Spectrometry Based Metabolomics Xiaoli Wei, Xue Shi, Seongho Kim, Li Zhang, Jeffrey S. Patrick, Joe Binkley, Craig McClain,,,,#

More information

ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS

ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS ION MOBILITY COUPLED TO HIGH RESOLUTION MASS SPECTROMETRY: THE POSSIBILITIES, THE LIMITATIONS Anton Kaufmann Official Food Control Authority of the Canton of Zurich (Kantonales Labor Zürich) Switzerland

More information

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides Mohammed Abrar Unilabs York Bioanalytical Solutions Outline Introduction Why LC-MS/MS? Limitations of

More information

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D

More information

Top 10 Tips for Successful Searching ASMS 2003

Top 10 Tips for Successful Searching ASMS 2003 Top 10 Tips for Successful Searching I'd like to present our top 10 tips for successful searching with Mascot. Like any hit parade, we will, of course, count them off in reverse order 1 10. Don t specify

More information

The area under the ROC curve as a variable selection criterion for multiclass classification problems

The area under the ROC curve as a variable selection criterion for multiclass classification problems The area under the ROC curve as a variable selection criterion for multiclass classification problems M. de Figueiredo 1,2 C. B. Y. Cordella 3 D. Jouan-Rimbaud Bouveresse 1,3 X. Archer 2 J.-M. Bégué 2

More information

New Methodologies for POPs Analysis using Orbitrap GC-MS in Official Food Control

New Methodologies for POPs Analysis using Orbitrap GC-MS in Official Food Control New Methodologies for POPs Analysis using Orbitrap GC-MS in Official Food Control Dr. Nuria Cortés-Francisco Servei de Química Laboratori Agència Salut Pública Barcelona Vancouver, August 22 nd, 217 Summary

More information

Content Part 2 Users manual... 4

Content Part 2 Users manual... 4 Content Part 2 Users manual... 4 Introduction. What is Kleos... 4 Case management... 5 Identity management... 9 Document management... 11 Document generation... 15 e-mail management... 15 Installation

More information

Discovering Meaningful Cut-points to Predict High HbA1c Variation

Discovering Meaningful Cut-points to Predict High HbA1c Variation Proceedings of the 7th INFORMS Workshop on Data Mining and Health Informatics (DM-HI 202) H. Yang, D. Zeng, O. E. Kundakcioglu, eds. Discovering Meaningful Cut-points to Predict High HbAc Variation Si-Chi

More information

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection Comparison of Relative Quantification of Monoclonal ntibody N-glycans Using Fluorescence and MS Detection pplication Note iotherapeutics & iologics uthors scar Potter and Gregory Staples gilent Technologies,

More information

Benefits and Characteristic Applications of High Resolution GC/MS and LC/MS. Frank David RIC and Ghent University

Benefits and Characteristic Applications of High Resolution GC/MS and LC/MS. Frank David RIC and Ghent University Benefits and Characteristic Applications of High Resolution GC/MS and LC/MS. Frank David RIC and Ghent University Mass Spectrometry Structure Elucidation Selective and Sensitive Detection Identification

More information

Thermo Fisher Scientific Inc., San Jose, CA; Bristol Myers Squibb, Princeton, NJ. Vast Scientific, Boston, MA

Thermo Fisher Scientific Inc., San Jose, CA; Bristol Myers Squibb, Princeton, NJ. Vast Scientific, Boston, MA Metabolomic Profiling in Drug Discovery: Understanding the Factors that Influence a Metabolomics Study and Strategies to Reduce Biochemical and Chemical Noise Mark Sanders 1 ;Serhiy Hnatyshyn 2 ; Don Robertson

More information

QSTAR Operation. May 3, Bob Seward

QSTAR Operation. May 3, Bob Seward QSTAR Operation May 3, 2005 Bob Seward Presentation CD Contents QSTAR Hardware QSTAR Schematic 10-7 torr QSTAR Schematic Chernushevich et al, JMS 2001, 36: 849-865 Curtain Plate ESI Source ESI Source Curtain

More information

Improving Selectivity in Quantitative Analysis Using MS 3 on a Hybrid Quadrupole-Linear Ion Trap Mass Spectrometer

Improving Selectivity in Quantitative Analysis Using MS 3 on a Hybrid Quadrupole-Linear Ion Trap Mass Spectrometer Improving Selectivity in Quantitative Analysis Using MS 3 on a Hybrid Quadrupole-Linear Ion Trap Mass Spectrometer Overview Increased selectivity is achieved in quantitative analysis by using MS 3. The

More information

1 Pattern Recognition 2 1

1 Pattern Recognition 2 1 1 Pattern Recognition 2 1 3 Perceptrons by M.L. Minsky and S.A. Papert (1969) Books: 4 Pattern Recognition, fourth Edition (Hardcover) by Sergios Theodoridis, Konstantinos Koutroumbas Publisher: Academic

More information

METABOSCAPE A METABOLITE PROFILING PIPELINE DRIVEN BY AUTOMATIC COMPOUND IDENTIFICATION

METABOSCAPE A METABOLITE PROFILING PIPELINE DRIVEN BY AUTOMATIC COMPOUND IDENTIFICATION METABOSCAPE A METABOLITE PROFILING PIPELINE DRIVEN BY AUTOMATIC COMPOUND IDENTIFICATION OR W TO LINK HRAM QTOF PLANT METABOLOMICS DATA TO BIOLOGY Aiko Barsch, Bruker Daltonics, Bremen, Germany 1 Outline

More information

Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry. Jianzhong Chen, PhD Assistant Professor School of Optometry UAB

Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry. Jianzhong Chen, PhD Assistant Professor School of Optometry UAB Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry Jianzhong Chen, PhD Assistant Professor School of Optometry UAB 1 Lipidome: A subset of Metabolome https://en.wikipedia.org/wiki/lipidomics

More information

Sue D Antonio Application Chemist Cedar Creek, TX

Sue D Antonio Application Chemist Cedar Creek, TX Sue D Antonio Application Chemist Cedar Creek, TX What is Hemp Oil? CBD hemp oil is a natural botanical extract of the common hemp plant. CBD hemp oil is derived from the seeds and stem of the Cannabis

More information

Agilent 1260 Infinity Analytical SFC System

Agilent 1260 Infinity Analytical SFC System Agilent 1260 Infinity Analytical SFC System Installation Instructions: Checkout Procedure This technical note describes the checkout procedures for the Agilent 1260 Infinity Analytical SFC System. Contents

More information

ProMax 3D Scanning Protocol

ProMax 3D Scanning Protocol ProMax 3D ProMax 3D Scanning Protocol Page 1 General Scanning Protocol for the ProMax 3D For Use with ident This protocol is written specifically for users of ProMax 3d CBCT scanner. Patient Scan with

More information

Low-level environmental phthalate exposure associates with urine metabolome. alteration in a Chinese male cohort

Low-level environmental phthalate exposure associates with urine metabolome. alteration in a Chinese male cohort Supporting Information Low-level environmental phthalate exposure associates with urine metabolome alteration in a Chinese male cohort Jie Zhang*, Liangpo Liu, Xiaofei Wang, Qingyu Huang, Meiping Tian,

More information

Benchmark Dose Modeling Cancer Models. Allen Davis, MSPH Jeff Gift, Ph.D. Jay Zhao, Ph.D. National Center for Environmental Assessment, U.S.

Benchmark Dose Modeling Cancer Models. Allen Davis, MSPH Jeff Gift, Ph.D. Jay Zhao, Ph.D. National Center for Environmental Assessment, U.S. Benchmark Dose Modeling Cancer Models Allen Davis, MSPH Jeff Gift, Ph.D. Jay Zhao, Ph.D. National Center for Environmental Assessment, U.S. EPA Disclaimer The views expressed in this presentation are those

More information

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique

More information

Early Learning vs Early Variability 1.5 r = p = Early Learning r = p = e 005. Early Learning 0.

Early Learning vs Early Variability 1.5 r = p = Early Learning r = p = e 005. Early Learning 0. The temporal structure of motor variability is dynamically regulated and predicts individual differences in motor learning ability Howard Wu *, Yohsuke Miyamoto *, Luis Nicolas Gonzales-Castro, Bence P.

More information

1. Sample Introduction to MS Systems:

1. Sample Introduction to MS Systems: MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:

More information

Target Analyses in Parallel Reaction Monitoring Mode (PRM)

Target Analyses in Parallel Reaction Monitoring Mode (PRM) Target Analses in Parallel Reaction Monitoring Mode (PRM) Skline Webinar Januar 13, 2015 Bruno Domon, PhD Head Luxembourg Clinical Proteomics Center Invited Professor Universit of Luxembourg INTRODUCTION

More information

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance Bruker Daltonics Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance The new ultraflextreme exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary khz smartbeam-ii TM MALDI

More information

Join the mass movement towards mass spectrometry

Join the mass movement towards mass spectrometry Join the mass movement towards mass spectrometry Thermo Scientific ISQ EC single quadrupole mass spectrometer Embrace the power of mass spectrometry Achieving a comprehensive understanding of the samples

More information

Learning Objectives. Overview of topics to be discussed 10/25/2013 HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS

Learning Objectives. Overview of topics to be discussed 10/25/2013 HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS A clinical proteomics perspective Michael L. Merchant, PhD School of Medicine, University of Louisville Louisville, KY Learning Objectives

More information

Rapid Screening and Quantitation of Postharvest Fungicides on Citrus Fruits Using AxION DSA/TOF and Flexar SQ MS

Rapid Screening and Quantitation of Postharvest Fungicides on Citrus Fruits Using AxION DSA/TOF and Flexar SQ MS APPLICATION NOTE Mass Spectrometry Author: Avinash Dalmia PerkinElmer, Inc. Shelton, CT USA Rapid Screening and Quantitation of Postharvest Fungicides on Citrus Fruits Using AxION DSA/TOF and Flexar SQ

More information

Why use Signal-To-Noise as a Measure of MS Performance When it is Often Meaningless?

Why use Signal-To-Noise as a Measure of MS Performance When it is Often Meaningless? Why use Signal-To-Noise as a Measure of MS Performance When it is Often Meaningless? Technical Overview Authors Greg Wells, Harry Prest, and Charles William Russ IV, Agilent Technologies, Inc. 285 Centerville

More information

PesticideScreener. Innovation with Integrity

PesticideScreener. Innovation with Integrity PesticideScreener A complete multi-residue solution for pesticide screening using high resolution full scan accurate mass data Innovation with Integrity LC-MS The Challenge of Ever Increasing Number of

More information

Identification of Ginsenosides Using the SCIEX X500R QTOF System

Identification of Ginsenosides Using the SCIEX X500R QTOF System Identification of Ginsenosides Using the SCIEX X500R QTOF System Wang Sha, Cheng Haiyan, Liu Ting, Li Lijun, Jin Wenhai[Author] SCIEX, Pacific Applications Support Center (Beijing). China Background Ginseng

More information

Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D

Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D Julie A. Horner 1, Marta Kozak 1, Subodh Nimkar 1, and August A. Specht 1 1 Thermo Fisher Scientific, San Jose,

More information

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies. Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in

More information

TOP-DOWN DETERMINATION OF SERUM INSULIN-LIKE GROWTH FACTORS BY LC-MS

TOP-DOWN DETERMINATION OF SERUM INSULIN-LIKE GROWTH FACTORS BY LC-MS TOP-DOWN DETERMINATION OF SERUM INSULIN-LIKE GROWTH FACTORS BY LC-MS Richard KT Kam, Ph.D. Scientific Officer, Department of Chemical Pathology Prince of Wales Hospital Prince of Wales Hospital, Hong Kong

More information

Bruker D8 Discover User Manual (Version: July 2017)

Bruker D8 Discover User Manual (Version: July 2017) University of Minnesota College of Science and Engineering Characterization Facility Eulerian cradle Reference 2θ (two-theta): Detector angle (GADDS angle # 1) Bruker D8 Discover User Manual (Version:

More information

A high performance mass spec designed just for chemists

A high performance mass spec designed just for chemists A high performance mass spec designed just for chemists Compact Mass Spectrometer The Advion CMS quickly provides essential information and improves the chemist s workflow. Modern organic labs have become

More information

Development and Validation of a Polysorbate 20 Assay in a Therapeutic Antibody Formulation by RP-HPLC and Charged Aerosol Detector (CAD)

Development and Validation of a Polysorbate 20 Assay in a Therapeutic Antibody Formulation by RP-HPLC and Charged Aerosol Detector (CAD) LIFE SCIENCES AR ENOUGH? HOW DO YOU KNOW RAW MATERIALS ARE PURE? HOW DO YOU EVALUATE PRODUCT PACKAGING? HOW DO YOU DO YOU KNOW WHAT ANALYTICAL TECHNIQUE TO USE? HOW DO YOU SIMULTANEOUSLY TEST FOR TWO BYPRODUCTS?

More information

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition

Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Evelyn Goh Waters Pacific, Singapore APPLICATION BENEFITS This method allows for the simultaneous analysis of 12 water-soluble

More information

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F

More information

Title: Prediction of HIV-1 virus-host protein interactions using virus and host sequence motifs

Title: Prediction of HIV-1 virus-host protein interactions using virus and host sequence motifs Author's response to reviews Title: Prediction of HIV-1 virus-host protein interactions using virus and host sequence motifs Authors: Perry PE Evans (evansjp@mail.med.upenn.edu) Will WD Dampier (wnd22@drexel.edu)

More information

Improved method for the quantification of lysophospholipids including enol ether

Improved method for the quantification of lysophospholipids including enol ether Supplemental Material Improved method for the quantification of lysophospholipids including enol ether species by liquid chromatography-tandem mass spectrometry James G. Bollinger *, Hiromi Ii*, Martin

More information

Background Information. Instructions. Problem Statement. HOMEWORK INSTRUCTIONS Homework #2 HIV Statistics Problem

Background Information. Instructions. Problem Statement. HOMEWORK INSTRUCTIONS Homework #2 HIV Statistics Problem Background Information HOMEWORK INSTRUCTIONS The scourge of HIV/AIDS has had an extraordinary impact on the entire world. The spread of the disease has been closely tracked since the discovery of the HIV

More information

Quantification with Proteome Discoverer. Bernard Delanghe

Quantification with Proteome Discoverer. Bernard Delanghe Quantification with Proteome Discoverer Bernard Delanghe Overview: Which approach to use? Proteome Discoverer Quantification Method What When to use Metabolic labeling SILAC Cell culture systems Small

More information

NMR ANNOTATION. Rémi Servien Patrick Tardivel Marie Tremblay-Franco Cécile Canlet 01/06/2017 v 1.0.0

NMR ANNOTATION. Rémi Servien Patrick Tardivel Marie Tremblay-Franco Cécile Canlet 01/06/2017 v 1.0.0 NMR ANNOTATION Rémi Servien Patrick Tardivel Marie Tremblay-Franco Cécile Canlet 01/06/2017 v 1.0.0 WHY DO WE NEED AN AUTOMATED NMR ANNOTATION TOOL 2 Metabolomic workflow Control mice Exposed mice Metabolites

More information

Application Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract.

Application Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling Application Note Food 1 Agilent 12 Infinity 1 Author Siji Joseph Agilent Technologies, Inc. Bangalore,

More information

High-Throughput Quantitative LC-MS/MS Analysis of 6 Opiates and 14 Benzodiazepines in Urine

High-Throughput Quantitative LC-MS/MS Analysis of 6 Opiates and 14 Benzodiazepines in Urine High-Throughput Quantitative LC-MS/MS Analysis of and 14 Benzodiazepines in Urine Bill Yu, Kristine Van Natta, Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 588 Key Words Opiates,

More information

Spot Colors Deep Dive in Fiery proserver and Fiery XF

Spot Colors Deep Dive in Fiery proserver and Fiery XF Spot Colors Deep Dive in Fiery proserver and Fiery XF Wide Format Series Kerry Moloney Field & Channel Marketing Manager Fiery Wide Format John Nate WW Technical Product Training Manager Fiery Wide Format

More information

Intro to HCI / Why is Design Hard?

Intro to HCI / Why is Design Hard? Intro to HCI / Why is Design Hard? September 12, 2016 Fall 2016 COMP 3020 1 Announcements A02 notes: http://www.cs.umanitoba.ca/~umdubo26/comp3020/ A01 notes: http://www.cs.umanitoba.ca/~bunt/comp3020/lecturenotes.html

More information

Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application

Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application Direct Analysis of Folic Acid in Digestive Juices by LC/TOF-MS Application Food Safety Authors M. H. Blokland, P. W. Zoontjes, S. S. Sterk, and L. A. van Ginkel, National Institute of Public Health and

More information

Analysis and Quantitation of Cocaine on Currency Using GC-MS/MS. No. GCMS No. SSI-GCMS-1501

Analysis and Quantitation of Cocaine on Currency Using GC-MS/MS. No. GCMS No. SSI-GCMS-1501 Gas Chromatograph Mass Spectrometer No. GCMS-1501 Analysis and Quantitation of Cocaine on Currency Using GC-MS/MS Shilpi Chopra, Ph.D., Laura Chambers Introduction Cocaine (CAS # 50-36-2), a white crystalline

More information

ERI User s Guide. 2. Obtaining the ERI for research purposes

ERI User s Guide. 2. Obtaining the ERI for research purposes ERI User s Guide 1. Goal and features of the ERI The Emotion Recognition Index (Scherer & Scherer, 2011) is a rapid screening instrument measuring emotion recognition ability. The ERI consists of a facial

More information

LC-Based Lipidomics Analysis on QTRAP Instruments

LC-Based Lipidomics Analysis on QTRAP Instruments LC-Based Lipidomics Analysis on QTRAP Instruments Junhua Wang and Paul RS Baker SCIEX LC-Based Lipidomics Analysis Topics Covered Lipid extraction techniques Hydrophilic Interaction Chromatography (HILIC)

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Sex-specific metabolic profiles of androgens and its main binding protein SHBG in a middle aged population without diabetes Uwe Piontek, Henri Wallaschofski, Gabi Kastenmüller,

More information

Crime Scene Investigation. Story

Crime Scene Investigation. Story Crime Scene Investigation Story Joe works as Detective in the Crime Scene Unit (CSU), which is a part of the SPD Detective Bureau's Forensic Investigations Division in Smallville City Police Department.

More information

FT-302 Force Transducer

FT-302 Force Transducer Technical Note FT-302 LabScribe is a trademark of 2015 Overview The FT-302 is a high-sensitivity dual-range research grade force transducer designed to measure forces in the 0.005 to 10 gram and 0 to 100

More information

PosterReprint OVERVIEW

PosterReprint OVERVIEW OVERVIEW A rapid multi-residue GC/MS method was developed for pesticides in foodstuffs. Plum extract was used as an example matrix. Analysis time was reduced from minutes to minutes. pesticides were analysed

More information

Automated Assessment of Diabetic Retinal Image Quality Based on Blood Vessel Detection

Automated Assessment of Diabetic Retinal Image Quality Based on Blood Vessel Detection Y.-H. Wen, A. Bainbridge-Smith, A. B. Morris, Automated Assessment of Diabetic Retinal Image Quality Based on Blood Vessel Detection, Proceedings of Image and Vision Computing New Zealand 2007, pp. 132

More information

Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease

Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease Multiplexed Precursor Ion Scanning and LipidView Software Brigitte Simons 1 and Bianca Arendt 2 1 AB SCIEX,

More information

Vitamin D3 and related compounds by ESI and APCI

Vitamin D3 and related compounds by ESI and APCI Liquid Chromatography Mass Spectrometry SSI-LCMS-9 Vitamin D and related compounds by ESI and APCI LCMS-8 Summary Vitamin D and related compounds were measured by LC-ESI/APCI-MS-MS. Background Accurate

More information

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No.

Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection. EPL-BAS Method No. Page 1 of 10 Determination of 6-Chloropicolinic Acid (6-CPA) in Crops by Liquid Chromatography with Tandem Mass Spectrometry Detection EPL-BAS Method No. 205G881B Method Summary: Residues of 6-CPA are

More information

ERO SCAN. Otoacoustic Emission Testing

ERO SCAN. Otoacoustic Emission Testing ERO SCAN Otoacoustic Emission Testing ERO SCAN OAE Testing for all ages Newborns School Children Toddlers Adults ERO SCAN Screening Version The ERO SCAN with screening function is the smart choice for

More information

MODULE: SQUAT JUMP BRIEF DESCRIPTION:

MODULE: SQUAT JUMP BRIEF DESCRIPTION: MODULE: SQUAT JUMP BRIEF DESCRIPTION: Squat jump is a vertical jump and among the most often used tests for maximal anaerobic power output of the lower extremities. It is a concentric muscle action which

More information

CDISC - Mapping New Pathways for Novartis. Darren Weston, Project Leader Pat Majcher, Senior Principal Programmer

CDISC - Mapping New Pathways for Novartis. Darren Weston, Project Leader Pat Majcher, Senior Principal Programmer CDISC - Mapping New Pathways for Novartis Darren Weston, Project Leader Pat Majcher, Senior Principal Programmer Shuhong hong Xie, Senior Statistical Programmer Agenda What was the submission request?

More information

Morita 3D Accuitomo Scanning Protocol

Morita 3D Accuitomo Scanning Protocol Morita 3D Accuitomo Scanning Protocol Page 1 Scanning Protocol for the Morita 3D Accuitomo General For Use with ident This protocol is written specifically for users of Morita 3D CBCT scanner. Patient

More information

Made For Routine Food, Environment, and Forensic Testing

Made For Routine Food, Environment, and Forensic Testing Made For Routine Food, Environment, and Forensic Testing The X500R QTOF System Powered by SCIEX OS High resolution mass spec, in perfect balance It s Time You Were Heard For far too long you ve had to

More information

Data mining for Obstructive Sleep Apnea Detection. 18 October 2017 Konstantinos Nikolaidis

Data mining for Obstructive Sleep Apnea Detection. 18 October 2017 Konstantinos Nikolaidis Data mining for Obstructive Sleep Apnea Detection 18 October 2017 Konstantinos Nikolaidis Introduction: What is Obstructive Sleep Apnea? Obstructive Sleep Apnea (OSA) is a relatively common sleep disorder

More information

The Development of LC/MS Methods for Determination of MDMA (Ecstasy) and Metabolites in Biological Samples

The Development of LC/MS Methods for Determination of MDMA (Ecstasy) and Metabolites in Biological Samples WA20714 The Development of LC/MS Methods for Determination of MDMA (Ecstasy) and Metabolites in Biological Samples Michael S. Young and Kevin M. Jenkins Waters Corporation, 34 Maple Street, Milford, MA

More information

Numerical Integration of Bivariate Gaussian Distribution

Numerical Integration of Bivariate Gaussian Distribution Numerical Integration of Bivariate Gaussian Distribution S. H. Derakhshan and C. V. Deutsch The bivariate normal distribution arises in many geostatistical applications as most geostatistical techniques

More information

Types of Modifications

Types of Modifications Modifications 1 Types of Modifications Post-translational Phosphorylation, acetylation Artefacts Oxidation, acetylation Derivatisation Alkylation of cysteine, ICAT, SILAC Sequence variants Errors, SNP

More information

Scanora 3D Scanning Protocol

Scanora 3D Scanning Protocol Scanora 3D Scanning Protocol Page 1 Scanning Protocol for the Scanora 3D For Use with ident General This protocol is written specifically for users of Scanora 3D CBCT scanner. Patient Scan with the appliance

More information

The Effect of LC-MS Data Preprocessing Methods on the Selection of Plasma Biomarkers in Fed vs. Fasted Rats

The Effect of LC-MS Data Preprocessing Methods on the Selection of Plasma Biomarkers in Fed vs. Fasted Rats Downloaded from orbit.dtu.dk on: Jan 28, 2018 The Effect of LC-MS Data Preprocessing Methods on the Selection of Plasma Biomarkers in Fed vs. Fasted Rats Gürdeniz, Gözde; Kristensen, Mette; Skov, Thomas;

More information

MSstatsQC: Longitudinal system suitability monitoring and quality control for proteomic experiments

MSstatsQC: Longitudinal system suitability monitoring and quality control for proteomic experiments MSstatsQC: Longitudinal system suitability monitoring and quality control for proteomic experiments Dr. Eralp DOGU Mugla University, TR Prof. Olga Vitek Group Northeastern University Skyline User Group

More information

Efficient Quantitative Analysis of THC and Metabolites in Human Plasma Using Agilent Captiva EMR Lipid and LC-MS/MS

Efficient Quantitative Analysis of THC and Metabolites in Human Plasma Using Agilent Captiva EMR Lipid and LC-MS/MS Application Note Forensic Analysis Efficient Quantitative Analysis of THC and Metabolites in Human Plasma Using Agilent Captiva EMR Lipid and LC-MS/MS Authors Joan Stevens and Limian Zhao Agilent Technologies,

More information

Pyxis MedStation System. Guide for Managing Patient-Specific Medication

Pyxis MedStation System. Guide for Managing Patient-Specific Medication Pyxis MedStation System Guide for Managing Patient-Specific Medication April 2012 CareFusion, Pyxis, Pyxis MedStation, and the CareFusion logo are trademarks or registered trademarks of CareFusion Corporation

More information

Quantitative Analysis of Opiates in Urine Using RRHT LC/MS/MS. Application. Authors. Introduction. Abstract. Forensics

Quantitative Analysis of Opiates in Urine Using RRHT LC/MS/MS. Application. Authors. Introduction. Abstract. Forensics Quantitative Analysis of piates in Urine Using RRHT LC/MS/MS Application Forensics Authors Sheher Mohsin Agilent Technologies, Inc. 10 N. Martingale Rd., Suite 550 Schaumburg, IL 60173 USA Yanan Yang Agilent

More information

Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition

Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition PO-CON1321E Quantitative Analysis of Carbohydrates and Artificial Sweeteners in Food Samples Using GFC- MS with APCI Interface and Post-column Reagent Addition ASMS 213 TP 71 Jie Xing 1, Yin Ling Chew*

More information

DPV. Ramona Ranz, Andreas Hungele, Prof. Reinhard Holl

DPV. Ramona Ranz, Andreas Hungele, Prof. Reinhard Holl DPV Ramona Ranz, Andreas Hungele, Prof. Reinhard Holl Contents Possible use of DPV Languages Patient data Search for patients Patient s info Save data Mandatory fields Diabetes subtypes ICD 10 Fuzzy date

More information