FISHERIES AND MARINE SERVICE. Translation Series No Investigation of the lipid composition of several species of fish

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1 FISHERIES AND MARINE SERVICE ARCHIVES Translation Series No Investigation of the lipid composition of several species of fish by A.G. Rudenko, and V.I. Lisovskaya Original title: Issledovanie sostava lipidov u nekotorykh vidov ryb From: Rybn. Khoz% (11):78-8, 1973 Translated by the Translation Bureau(JTM) Multilingual Services Division Department of the Secretary of State of Canada Department of the Environment Fisheries and Marine Service Halifax Laboratory Halifax, N.S pages typescript

2 , DEPARTI,AENT OF THE SECRETARY OF STATE TRANSLATION BUREAU e SECRÉTARIAT D'ÉTAT BUREAU DES TRADUCTIONS.; 7 MULTILINGUAL SERVICES DIVISION DES SERVICES CANADA DIVISION ' MULTILINGUES TRANSLATED FROM - TRADUCTION DE Russian AUTHOR - AUTEUR Lisovskaya V.I. and Rudenko A.G. INTO - EN English TLE IN ENGLISH - TITRE ANGLAIS Investigation of the lipid composition of several species of fish TITLE IN FOREIGN LANGUAGE (TRANSLITERATE FOREIGN CHARACTERS) TITRE EN LANGUE ÉTRANGÈRE (TRANSCRIRE EN CARACTÉRES ROMAINS) Issledovanie sostava lipidov u nekotorykhvidov ryb REFERENCE IN FOREIGN LANGUAGE (NAME OF BOOK OR PUBLICATION) IN FULL. TRANSLITERATE FOREIGN CHARACTERS. RÉFÉRENCE EN LANGUE ÉTRANGÈRE (NOM DU LIVRE OU PUBLICATION), AU COMPLET, TRANSCRIRE EN CARACTÉRES ROMAINS. Rybnoe khozyaistvo REFERENCE IN ENGLISH - RÉFÉRENCE EN ANGLAIS Fishery PUBLISHER - ÉDITEUR DATE OF PUBLICATION DATE DE PUBLICATION PAGE NUMBERS IN ORIGINAL NUMÉROS DES PAGES DANS L'ORI GINAL PLACE OF PUBLICATION LIEU DE PUBLICATION Mos cow YEAR ANNÉE 73 VOLUME ISSUE NO. NUMÉRO NUMBER OF TYPED PAGES NOMBRE DE PAGES DACTYLOGRAPHIÉES 4 REQUESTING DEPARTMENT MINISTRE-CLIENT EnVir 11Ment TRANSLATION BUREAU NO. NOTRE DOSSIER N BRANCH OR DIVISION DIRECTION OU DIVISION Fisheries Service TRANSLATOR (INITIALS) TRADUCTEUR (INITIALES) JTM PERSON REQUESTING Dr.. R.G. Ackman DEMAND PAR YOUR NUMBER VOTRE DOSSIER N DATE OF REQUEST DATE DE LA DEMANDE 12 Dec 75 Fzir rl'zt4i:juctic.:4` only len NON rup,,,ie.l..e. SOS 2 1. (REV. 2/6)

3 ' DEPARTMENT OF THE SECRETARY OF STATE TRANSLATION BUREAU. MULTILINGUAL SERVICES DiVISION ç, - eçey,'?,1 CANADA SECRUTARIAT D'ÉTAT BUREAU DES TRADUCTIONS DIVISION DES SERVICES MULTILINGUES. CLIENT'S NO. ' DEPARTMENT DIVISION/BRANCH CITY NO DU CLIENT MINISTÉRE DIVISION/DIRECTION VILLE Environment Fisheries Service Ottawa BUREAU NO. LANGUAGE TRANSLATOR (INITIALS) N DU BUREAU LANGUE TRADUCTEUR (INITIALES) Russian JTM FEB $ UDC Investigation of the lipid composition of several species of fish By: Lisovskaya,V.I. and Rudenko,A.G. Odessa Division of the Institute of Biology of the Southern Seas, Academy of Sciences of the UkSSR I!J () rr.; e 7: C) d*'-' () E (;) '' CI 15.1 e;j: The scarcity of information in the literature concerning the lipid composition of marine fish prompted us to conduct an investigation of the lipid composition in the muscle tissue, liver and roe of six species of fish: bonefish, bronze sea bass, notched grunt, sea bream, drepane and flatfish. The biological description and data'obtained about the relative weight of their gonads and livers are given in table 1. For most of the fish investigated, the roe and milt were at the IV - V stages of ripeness and comprised no more than 3.6% of the total weight of the fish. The liver did not exceed 1% of the fish weight. Table 2 contains data on the amount of dry matter and free fat extracted on ether by the Soxlet method, and also the total amount of all the lipids extracted from. the muscle tissue, roe and liver of the fish using the Folch method. It can be seen from the table that the /79 amount of free fat and total lipid content in the muscles of different fish species fluctuates between significant limits. It is very characteristic

4 Table 1 Weight of intern al organs in % of total we ight of fish Fish species Sex Weight roe milt liver 1. Bonefish 2. Bronze sea bass 3. Notched grunt 4. Ehrenbergs's sea 5. Drepane 6. Flatfish fe bream fe fe fe that all the fish investigated were richer in liver and roe lipids than in those of muscle (about 1-3 times). When the amountsof free fat and ail the lipids in the muscles and liver of sea bass and flatfish of different sexes were compared, no essential difference was detected. In the liver of the bronze sea bass the ether-soluble (free) fats comprise about one third of all the lipids, while in the liver of the sea bream and flatfish they make up about one half. The amount of dry matter in the muscles of all the fish investigated is about double that in the liver. The lipids extracted from the muscles and liver of the fish by the Folch method were divided into fractions by thin layer chromatography. Quantitative interpretation of the chromatograms was done on the MF-4 spectrophotometer. The results are shown in table 3.

5 - - Table 2 3 a6-a mg a 2' 1Anb5y.na BHA pte5bi ( a) 2 Benononocbul mpoy 3 Ilpncrinoma,LI.Tninnonepbirt napr 4 Apenàna 6 neerrce non CamenT Camna8 Camenrï Camxati Camna 6-beKT ncencaonannn ( c ) Mblalgbi 9 MOROKII 1 n negerb MbIW1Ibl fleenb 11 Iinpa rienenb 111b! l'iihnught' 9 l'inpai 2 Iletient.,..,1 1.bibunizbbil Ilnpa 12 9 Hnpal2 MÉnnubi Helen 11 CoAemanne cxxnx nernectn. % OT cupoil macchz ( d) 22,9-11, 15,6 9,9 2,9 1,6 25,8 24,3 9,5 22,9 12,3 21, 23,8 12,3 Neplxamte annn,qou, % OT cyxoro netnec -rna 3+11npopacr- IMplIMNX no CoRenery fi I / 2,5. 1,4. 26,6 1,6 16,3-21,9 2,4 1,7 1,1 2,7 1,2 13,9,9 21,9 1,4 2,7 of5uptx no <Ponqy q?-9 23,8 2,3 63,4 2,4 71,8 4, 1,8 49,8 1,3 62,3 1,4 1,6 49,8 Key: a- fish species; b- sex; c- subject; d- amount of dry matter in % of raw weight; e- amount of lipids in % of dry matter; f- ether solubles by Soxlet method; g- total by Folch method: 1 to 6- as for table 1; 7- ; 8- fe; 9- muscle; 1- mut; 11- liver; 12- roe. BnA pm6u ( a) Anb6y.na 1:. 13e.gono.noemil m9p,2 ' To :ne 'Dittb IlpneTnnoma 3 Anninionepuri narp 4..{1penana 5 ncettoa» 6 Anb6yza Be.nono.nocuil eppoy 2 To n:e nplictunoma Dtto i 3..annnnonephnl narp Lipenana 4 ficerro,1 6 5,non ( b ) cameu 7 Ca MK a 8 cameu 7 Camna 8 Cam= 7 Camel( 7 Camxa 8 Camen 7 Camxa 8 Caren 7 2 4, 31,4 22,7 4,1 27,7 2,2 M 39,5 43,4 48, 37,3 53,1 44,3 56,3 49,8 ii 2 M ( 111 ) % OT Ile gelib 5,1 4,4 4,6 7,2 8,3 4,2 e. a z f nma n- X Liver 22,2 2,5 2,6 4,3 11,8 6,5 11,2 3,9 13,2 7,8 6,5 2,7 2,6 23,7 33,9 17,3 16, ,1 21,5 Muscle F.< ( h) -e oew,ero Konngectna BCCX.nnrn Awl 14,5 13, 9,8 14,2 16,8 25,4 1,3 23, 8,5 26,7 35,8 37,4 37,2 31,5 17,2 5, , çae;ui tra ça 8,9 2,3 4,3 29, 3,4 es Table 3 ra6aina 3 (.1)1 ( o. mu 3, 3,6 o t=c 2 >a I.( - 11.,9 3,6 6,7 7,8 7, _ _ - Caura Traces - 5,8 C Tem:4 Traces 8,1 11,2 5,. 12,2 14,1 15,1 1,8 6,4 Key: a- fish species; b- sex; c- phospholipids; d- monoglycerides; e- cholesterol; f- free fatty acids; g- triglycerides; h- vitamin A; J- sterol esters; k- carbohydrates; m- % of total of all lipids; 1 to 8- as for table 2.

6 4 The composition of the lipids extracted frdm fish liver was found to contain phospholipids, mono- and diglycerides, cholesterol, free fatty acids, triglycerides, vitamin A, sterol esters, carbohydrates and some unidentified substances. The composition of the muscle lipids was found to be poorer, they included phospholipids, cholesterol, free fatty acids, triglycerides, carbohydrates and some unidentified substances. It is possible that some of the other lipid fractions. found in liver are also present in the muscles, but in very small quantities and were not detected in these experimental conditions. Table 3 shows that the liver and muscles of the investigated fish are characterized by high phospholipid and cholesterol content, the proportion of which in muscles is from 54.6 to 81.9% of the total lipids; and in the liver of different fish, with the exception of flatfish, it is 34.5 to 62.2% (26.7% in flatfish liver). The high content of triglycerides in fish liver is also noted; they comprise from 26.7 to 37.4% of the total lipids, bonefish excluded, the lipid composition in the liver of which was found to be 8.5% triglycerides. The muscles of most of the fish investigated were found /8 to contain insignificant amounts of triglycerides, and only in bonefish and notched grunt did they comprise respectively 17.2 and 12.% of all lipids. The free fatty acids in the composition of muscles lipids was significantly greater (from.9.8 to 25.4%) than in liver (2.5 to 7.8%). Considering the intensity of metabolic processes taking place in the liver, it is easily understood why it contains fractions that are completely absent from the muscles, or else are thenain insufficient quantities, such as the mono- and diglycerides, vitamin A, and sterol esters.

FISHERIES.AND MARINE SERVICE

FISHERIES.AND MARINE SERVICE Trànslation Series No, 3638 Search for preservatives for storage of caught fish by G.G. Skovortsova, E.S. Domnina, Yu.A. Mansurov, G.N. Kurov, and N.P. Glazkova Original title: