Photo: USDA. DHA Natur (ADM Dried Algae) Biomass rich in DHA
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2 Photo: USDA DHA Natur (ADM Dried Algae) Biomass rich in DHA
3 DHA Growth Limiting Factor? Sustainable fish oil alternative needed Supply challenges for fish oil increase price & environmental concern Except for El Niño events, global supply of fish oil has been stagnant at ~1.3 MMT best case scenario it is predicted to remain stable. Salmon feed utilizes 50% of the fish oil FAO: 25% of world s fish stocks are either overexploited or depleted, and 52% of the stocks are fully exploited Demand for food and supplemented Ω-3 is estimated to increase 10% by 2016 according to EuroMonitor Demand from the salmon feed industry is particularly strong with a projected increase of 55% from 2012 to 2020
4 That`s Why: DHA Natur Rich in DHA The need for DHA is increasing and the health benefits of Omega-3 (EPA/DHA) are accepted by nutritionally-conscious consumers ADM has dedicated a large production facility to the production of a novel, globally-competitive DHA source ADM s DHA Natur is a viable ingredient suitable to fulfill DHA requirements for all species, but it is most favorable for marine fish including salmonids
5 DHA and EPA (NRC 2011 Table 19-5) Fish species DHA EPA EPA + DHA* % of total Fatty Acids Anchovy Capelin Cod liver Herring (Atlantic) Menhaden Sardine Tuna orbital DHA Natur
6 EPA and DHA Requirements Salmon and Humans Salmon have a requirement for EPA + DHA of 1% for optimum growth (NRC 2011 Table 6-1) BUT: A higher dietary inclusion is necessary for suitable deposition in fillet tissue to attain the WHO-recommended DHA intake* via one weekly serving of salmon (~ 6% EPA + DHA in dietary lipid fraction) *World Health Organization recommendation is mg/day EPA+DHA
7 DHA Natur Genus Aurantiochytrium (formerly Schizochytrium) Microalgae unicellular heterotrophic species Vegetarian source of DHA with minimum 17% DHA ADM s technology allows heterotrophic algae to be produced more efficiently than phototrophic algae ADM has developed analytical method for accurate testing of DHA level in algae products
8 Product Specs DHA Natur is a dried algal biomass rich in docosahexaenoic acid (DHA). It is a highly concentrated, vegetarian source of DHA, an Omega-3 fatty acid, derived from algae through an efficient, controlled fermentation process that does not require light. ADM s microalgae-based technology allows heterotrophic algae to be produced efficiently. Typical Analysis Test Method Crude protein, min... 5% ISO :2005 Total Fat as FAME, min./max % AM 102 (ADM, Decatur, IL, USA) (FAME Fatty Acid Methyl Esters) DHA content, min % AM 102 (ADM, Decatur, IL, USA) Moisture, max % ISO 6496:1999 Ash % Carbohydrate (galactose) 28%
9 Bio-efficacy Univ. Ås Norway Salmon Trial ADC-DHA: comparison against cod liver oil CLO coated after extrusion algae passed through extruder Digestibility of DHA in algae biomass was 91% that of DHA in cod liver oil
10 ADM Trial Nutrition and Digestibilily Code: A
11 Experimental setup Fundación Chile, Quillaipe, Chile Atlantic salmon, Salmo salar Size: 140 g at trial start Tanks: 1,000 L conical bottom Temperature: 17 C Dissolved oxygen: > 80% saturation Salinity: 32 ppt Photoperiod: 24 h light Feeding: ad libitum AM & PM Density: 9 40 kg/m 3 90-day growth trial 14-day digestibility trial Experimental diets Growth trial: Four experimental diets 55% crude protein, 20% lipid Iso proteic & Iso lipidic Golden Extruded (BioMar) + DHA 0, 1.8, 3.7 and 5.5% of diet Weighing: Beginning, monthly and end Digestibility trial: 70:30 reference diet: DHA Natur 0.01% Yttrium oxide Manual stripping every week
12 Results Weight gain Best growth diet with 3.7% DHA Natur (diet 3) Average weight (g) Diet 1 Diet 2 Diet 3 Diet 4 T0 T30 T60 T90 % ADC Digestibility DHA % ADC Algae meal Crude fat Further ongoing trials Crude protein DHA Crude fat Crude protein Rainbow trout: In progress at Fish Technology Center, USDA-ARS, Bozeman, MT Atlantic salmon: In progress at Cool & Cold Water Aquaculture Center, USDA-ARS, Franklin, ME
13 DHA Natur Production Corn Dextrose Vitamins, Minerals Micro Algae Fermentor Algae Broth Packaging Dryer Liquid Biomass Evaporation Product is packaged in oxygen impermeable, nitrogen-purged, high-quality, 500-kg super sacks Antioxidants
14 GMP, Food-grade facility Production Facility Produced from a controlled fermentation process Produced at ADM s facility in Clinton, Iowa, USA Large capacity production
15 Production Facility Clinton, Iowa, USA
16 Product Packaging High-quality, 500-kg, multi-lined super sack; also available in 20-kg bags Product is stabilized with natural antioxidants (citric acid and MTS) and kept in oxygen impermeable, nitrogen-purged packaging Unsaturated fatty acids are vulnerable to oxidation rancidity Physical Characteristics Appearance: tan, granular powder Aroma: marine Storage & Handling DHA Nature should be kept in a dry, cool and well-ventilated storage facility where direct sunlight can be prevented. When stored in unopened original containers and under recommended storage conditions, the shelf life is minimum six months
17 Features and Benefits Summary A concentrated algae Vegetarian source of DHA Efficient production process Manufactured under controlled fermentation GMP production that does not require light (heterotropic fermentation) DHA Natur is free of heavy metals and pesticides ADM quality standards and single-site production guarantee consistency DHA Natur is approved in the EU, Chile, Australia and New Zealand Registrations initiated in the USA, Canada and other countries
18 Nutritional Profile Physical Characteristics Description: DHA Natur (ADM Dried Algae) is a highly concentrated, vegetarian source of DHA, an Omega-3 fatty acid. Appearance: Tan, fine granular meal European Union (EU) Approved DHA Natur (ADM Dried Algae) feed-grade algae is approved in the EU: Commission Regulation (EU) 68/2013 PART C List of Feed Materials No Also approved in Chile, Australia and New Zealand Gross Energy (GE) kcal/g (as-is 97.6% dry matter) Directions for Use DHA Natur is well suited for all terrestrial and aquatic species where a need for dietary Omega-3 fatty acids, in particular DHA, has been established Storage & Handling DHA Natur should be kept in a dry, cool and wellventilated place away from direct sunlight. When stored in closed or sealed, airtight original packaging and under recommended storage conditions, the shelf life is a minimum six months
19 Nutritional Profile continued Fatty Acids Profile 31-36%.... DHA - C22:6n3 (based on lipid portion of DHA Natur) 17-20%.... DHA - C22:6n3 (based on as is basis of DHA Natur) Fatty acid composition available upon request. Amino Acid Concentrations (%) Alanine 0.31 Arginine 0.26 Aspartic Acid 1.00 Cysteine 0.23 Glutamic Acid 0.65 Glycine 0.25 Histidine 0.12 Hydroxylysine 0.03 Hydroxyproline 1.33 Isoleucine 0.23 Lanthionine 0.00 Leucine 0.37 Lysine 0.29 Methionine 0.18 Ornithine 0.01 Phenylalanine 0.21 Proline 0.18 Serine 0.29 Taurine 0.19 Threonine 0.32 Tryptophan 0.08 Tyrosine 0.15 Valine 0.30
20 Thank you Photos: USDA The information contained herein is correct as of the date of this document to the best of our knowledge. Any recommendations or suggestions are made without guarantee or representation as to results and are subject to change without notice. We suggest you evaluate any recommendations and suggestions independently. WE DISCLAIM ANY AND ALL WARRANTIES, WHETHER EXPRESS OR IMPLIED, AND SPECIFICALLY DISCLAIM THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, AND NON-INFRINGEMENT. Our responsibility for claims arising from any claim for breach of warranty, negligence, or otherwise shall not include consequential, special, or incidental damages, and is limited to the purchase price of material purchased from us. None of the statements made here shall be construed as a grant, either express or implied, of any license under any patent held by Archer Daniels Midland Company or other parties. Customers are responsible for obtaining any licenses or other rights that may be necessary to make, use, or sell products containing Archer Daniels Midland Company ingredients. 0916
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