The Golgi Apparatus: Shipping and Receiving Center. The Golgi apparatus. Functions of the Golgi apparatus. Lysosomes: Digestive Compartments

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1 The Golgi Apparatus: Shipping and Receiving Center The Golgi apparatus Receives (on the cis-side) many of the transport vesicles produced in the rough ER Consists of flattened membranous sacs called cisternae Exports many substances (from the transside) in transport vesicles Functions of the Golgi apparatus Golgi apparatus - Modification of the products of the rough ER cis face ( receiving side of Golgi apparatus) - Manufacture of certain macromolecules 1 Vesicles move 2 6 Vesicles also from ER to Golgi transport certain proteins back to ER Vesicles coalesce to form new cis Golgi cisternae Cisternae 3 Cisternal maturation: Golgi cisternae move in a cisto-trans direction -Probably evolved from ER µm Figure Vesicles transport specific proteins backward to newer Golgi cisternae 4 Vesicles form and leave Golgi, carrying specific proteins to other locations or to the plasma for secretion trans face ( shipping side of Golgi apparatus) TEM of Golgi apparatus Lysosomes: Digestive Compartments Lysosomes are membranous sacs of hydrolytic enzymes, and they carry out intracellular digestion. They digest food from food vacuoles that form by phagocytosis and they recycle old cell parts in autophagy. Lysosome Lysosome contains active hydrolytic enzymes Food vacuole fuses with lysosome Digestive enzymes Nucleus 1 µm Hydrolytic enzymes digest food particles Lysosome Plasma Digestion Food vacuole Figure 6.14 A (a) Phagocytosis: lysosome digesting food 1

2 Lysosomes different lysosomes have different enzymes for breaking down different macromolecules Lysosome containing two damaged organelles 1 µ m They have a low ph (around 5); pump H + ions in from the cell Example of a lysosomal disease: Tay-Sachs disease, caused by a missing lysosomal enzyme for lipid breakdown, leads to buildup of lipids in the brain, killing the individual in infancy. Figure 6.14 B Mitochondrion fragment Peroxisome fragment Lysosome fuses with Hydrolytic enzymes vesicle containing digest organelle damaged organelle components Lysosome Digestion Vesicle containing damaged mitochondrion (b) Autophagy: lysosome breaking down damaged organelle Vacuoles: Diverse Maintenance Compartments Vacuoles are fluid filled and enclosed. A cell may have one or several vacuoles. Food vacuoles Are formed by phagocytosis Contractile vacuoles Pump excess water out of protist cells Vacuoles: Diverse Maintenance Compartments Central vacuoles Found in plant cells Function in cell size and turgidity Store reserves of important organic compounds and water Central vacuole Cytosol Figure 6.15 Nucleus Cell wall Chloroplast Tonoplast Central vacuole 5 µm 2

3 The Endo System: A Review Relationships among s/organelles of the endo system 1 Nuclear envelope is connected to rough ER, which is also continuous with smooth ER Nucleus Rough ER 2 Membranes and proteins produced by the ER flow in the form of transport vesicles to the Golgi Smooth ER Nuclear envelop cis Golgi 3 Golgi pinches off transport Vesicles and other vesicles that give rise to lysosomes and Vacuoles trans Golgi Plasma Figure Lysosome available 5 Transport vesicle carries 6 Plasma expands for fusion with another proteins to plasma by fusion of vesicles; proteins vesicle for digestion for secretion are secreted from cell Organelles of Endosymbiotic Origin Mitochondria and chloroplasts change energy from one form to another Mitochondria Are sites of cellular respiration Plastids Found only in plants, are sites of photosynthesis Mitochondria: Chemical Energy Conversion Mitochondria (powerhouse of the cell) Are found in nearly all eukaryotic cells Have their own DNA- derived from the mother. This DNA changes very slowly over time because there is no recombination, only change is due to drift (chance). Mitochondrion Inter space Outer Free ribosomes in the mitochondrial matrix Inner Cristae Matrix Figure 6.17 Mitochondrial DNA 100 µm 3

4 Mitochondria: Chemical Energy Conversion Are the site of oxidative metabolism (conversion of glucose to ATP, carbon dioxide, and water), also known as cellular respiration. * Which type of cell would you expect to have a lot of mitochondria? Are enclosed in a double. Inner is folded for increased surface area. This is where the metabolism occurs; enzymes are embedded in the. Mitochondrion Inter space Outer Free ribosomes in the mitochondrial matrix Inner Cristae Matrix Figure 6.17 Mitochondrial DNA 100 µm Plastids: Capture of Light Energy Plastids have a double have their own DNA function in photosynthesis (the chloroplast is an example) contain pigments such as chlorophyll, carotenoids can also be for storage (leukoplasts) Chloroplasts Are found in leaves and other green organs of plants and in algae Their structure includes Thylakoids, membranous sacs Stroma, the internal fluid Chloroplast Chloroplast DNA Ribosomes Stroma Inner and outer s Granum Figure 6.18 Thylakoid 1 µm 4

5 Peroxisomes: Oxidation Peroxisomes Produce hydrogen peroxide and convert it to water Chloroplast Peroxisome Mitochondrion Figure µm The Cytoskeleton Cytoplasm includes all the space inside the plasma but outside the nucleus (includes organelles, cytosol, and cytoskeleton) Cytoskeleton: microlattice of fibers supports the cell and gives it 3-dimensional shape. Organelles attach to the fibers. The cytoskeleton gives the cell spatial information, which is very important in development The cytoskeleton is not stationary, it is dynamic. The Cytoskeleton Is a network of fibers extending throughout the cytoplasm, and it organizes cell structures and activities. Microtubule Figure µm Microfilaments 5

6 Roles of the Cytoskeleton: Support, Motility, and Regulation Gives mechanical support to the cell Is involved in cell motility, which utilizes motor proteins ATP Vesicle Receptor for motor protein Motor protein Microtubule (ATP powered) of cytoskeleton (a) Motor proteins that attach to receptors on organelles can walk the organelles along microtubules or, in some cases, microfilaments. Microtubule Vesicles 0.25 µm Figure 6.21 A, B (b) Vesicles containing neurotransmitters migrate to the tips of nerve cell axons via the mechanism in (a). In this SEM of a squid giant axon, two vesicles can be seen moving along a microtubule. (A separate part of the experiment provided the evidence that they were in fact moving.) Components of the Cytoskeleton There are three main types of fibers that make up the cytoskeleton Table 6.1 Microtubules Microtubules Shape the cell Cilia and flagella for motility Guide the movement of organelles Help separate the chromosome copies in dividing cells 6

7 Centrosomes and Centrioles The centrosome Is considered to be a microtubule-organizing center and it organizes the spindle fibers used to guide the movement of chromosomes during cell division. In animal cells, the centrosome: Contains a pair of centrioles which are made of microtubules in a nine-triplets pattern. Centrosome Microtubule Centrioles 0.25 µm Figure 6.22 Longitudinal section of one centriole Microtubules Cross section of the other centriole Cilia and flagella locomotory organelles Cilia and flagella share a common ultrastructure of microtubules in a arrangement. The base structure is similar to that of centrioles (nine triplets). 0.1 µm Outer microtubule doublet Dynein arms Plasma Microtubules Plasma Central microtubule Outer doublets cross-linking proteins inside Radial spoke Basal body (b) (a) 0.5 µm 0.1 µm Triplet (c) Figure 6.24 A-C Cross section of basal body 7

8 Cilia and Flagella move through the action of motor proteins The protein dynein Is responsible for the bending movement of cilia and flagella Microtubule doublets ATP Dynein arm (a) Powered by ATP, the dynein arms of one microtubule doublet grip the adjacent doublet, push it up, release, and then grip again. If the two microtubule doublets were not attached, they would slide Figure 6.25 A relative to each other. Ciliary/flagellar motion Outer doublets cross-linking proteins ATP Anchorage in cell (b) In a cilium or flagellum, two adjacent doublets cannot slide far because they are physically restrained by proteins, so they bend. (Only two of Figure 6.25 B the nine outer doublets in Figure 6.24b are shown here.) Microfilaments (Actin Filaments) Are built from molecules of the protein actin Are found in microvilli Microvillus Plasma Microfilaments (actin filaments) Intermediate filaments Figure µm 8

9 Microfilaments of muscle Microfilaments that function in cellular motility Contain the protein myosin in addition to actin Muscle cell Actin filament Myosin filament Myosin arm Figure 6.27 A (a) Myosin motors in muscle cell contraction. Amoeboid motion Involves the contraction of actin and myosin filaments Cortex (outer cytoplasm): gel with actin network Inner cytoplasm: sol with actin subunits Extending pseudopodium Figure 6.27 B (b) Amoeboid movement Cytoplasmic streaming Is another form of locomotion created by microfilaments Streaming cytoplasm (sol) Nonmoving cytoplasm (gel) Chloroplast Parallel actin filaments Cell wall Figure 6.27 C (b) Cytoplasmic streaming in plant cells 9

10 Intermediate Filaments Support cell shape Fix organelles in place Are fixed and do not disassemble. Extracellular components and connections between cells help coordinate cellular activities Cell Walls of Plants Extracellular structures of plant cells that distinguish them from animal cells Are made of cellulose fibers embedded in other polysaccharides and protein May have multiple layers Central vacuole of cell Central vacuole of cell Plasma Secondary cell wall Primary cell wall Middle lamella 1 µm Central vacuole Cytosol Plasma Plant cell walls Figure 6.28 Plasmodesmata 10

11 The Extracellular Matrix (ECM) of Animal Cells Animal cells Lack cell walls Are covered by an elaborate matrix, the ECM The ECM Is made up of glycoproteins and other macromolecules. Some of these molecules can be part of self-recognition or - interactions (e.g. tissue glue that holds cells together). Collagen Fibronectin EXTRACELLULAR FLUID A proteoglycan complex Microfilaments Polysaccharide molecule Carbohydrates Core protein Plasma Integrins Proteoglycan molecule Integrin Figure 6.29 CYTOPLASM Functions of the ECM include Support Adhesion Movement Regulation 11

12 Intercellular Junctions in Plants Plasmodesmata are channels that perforate plant cell walls. The cell s of neighboring cells are continuous through these pores in the cell walls. This allows cells to share molecules and communicate. Cell walls Interior of cell Interior of cell Figure µm Plasmodesmata Plasma s Animal Cell Junctions In animals, there are three types of intercellular junctions Tight junctions Desmosomes Gap junctions Animal Cell Junctions Types of intercellular junctions in animals TIGHT JUNCTIONS Tight junctions prevent fluid from moving across a layer of cells Tight junctions Intermediate filaments Desmosome Tight junction At tight junctions, the s of neighboring cells are very tightly pressed against each other, bound together by specific proteins (purple). Forming continuous seals around the cells, tight junctions prevent leakage of extracellular fluid across A layer of epithelial cells. 0.5 µm DESMOSOMES Desmosomes (also called anchoring junctions) function like rivets, fastening cells Together into strong sheets. Intermediate Filaments made of sturdy keratin proteins Anchor desmosomes in the cytoplasm. Gap junctions 1 µm GAP JUNCTIONS Space between Plasma s cells of adjacent cells Figure 6.31 Extracellular matrix Gap junction 0.1 µm Gap junctions (also called communicating junctions) provide cytoplasmic channels from one cell to an adjacent cell. Gap junctions consist of special proteins that surround a pore through which ions, sugars, amino acids, and other small molecules may pass rapidly. Gap junctions are necessary for communication between cells in many types of tissues, including heart muscle and animal embryos. 12