Quantification of Free Sterols, Sterol Esters, Sterol Glucosides and Acylated Sterol Glucosides in Plants by

Size: px
Start display at page:

Download "Quantification of Free Sterols, Sterol Esters, Sterol Glucosides and Acylated Sterol Glucosides in Plants by"

Transcription

1 Quantification of Free Sterols, Sterol Esters, Sterol Glucosides and Acylated Sterol Glucosides in Plants by Q-TOF Mass Spectrometry Vera Wewer and Peter Dörmann Seville, Spain, July 2012 Institute of Molecular Physiology and Biotechnology of Plants (IMBIO) University of Bonn

2 Phytosterols: Structural Variety Cholesterol: the most abundant sterol in animals In plants, β-sitosterol and stigmasterol are predominent. Campesterol is the precursor for brassinosteroid synthesis. Ergosterol, a fungal sterol

3 Conjugated Sterol lipids

4 Sterol Distribution in the Plant Cell Free Sterols (FS) HO Sterol Esters (SE) Sterol Glucosides (SG) Acylated Sterol Glucosides (ASG)

5 The need to develop a comprehensive method for sterol measurements Changes of sterol lipids: Abiotic stress: -P, -N, senescence,... Biotic stress: pathogens (bacteria, fungi), symbiotic interactions (mycorrhiza, nodulation) Traditional method: TLC separation + quantification of sterols or fatty acids by GC or GC-MS

6 Strategy to measure all 4 sterol lipid classes Direct infusion MS/MS similar to the lipidomics measurements by Ruth Welti Avoid time consuming LC procedures Nanospray (low sample amount): 1 µ/min Lipid enrichment prior to MS: SPE with silica columns Use two internal standards (small, large) to correct for size dependence of signal Use saturated standards to measure the plant sterols (which are unsaturated)

7 Sterol Analysis: Workflow Q-TOF MS TLC / GC Wewer et al 2011 Wewer et al., 2011 J.Lip.Res.

8 Tandem Mass Spectrometry Direct infusion of lipid extract Selection of desired ions in Q1 Fragmentation of selected ions in Q2 (MS/MS) Time of Flight separation of fragment ions

9 Problems to overcome: Standards Free sterols: Saturated sterols: commercially available Sterol Esters: cholesterol esters: commercial available Sterol glucosides: synthesized ed from free saturated ated sterols s and glucopyranosyl-bromide-tetrabenzoate Acylated sterol glucosides saturated ASG: hydrogenation of ASG from soybean

10 Fragmentation Patterns of Sterols Free Sterols (FS) (betainylated) Improve ionization Introduce good Leaving group Sterol Esters (SE) Use of cholesterol esters as internal standards Sterol glucosides (SG) Acylated sterol glucosides (ASG) Saturated ASGs and SGs fragment differently than unsaturated ones

11 ASG Molecular Species ASG Arabidopsis leaf 16:0-Glc-Sitosterol 18:3-Glc-Sitosterol 18:2-Glc-Sitosterol

12 SE Molecular Species SE Arabidopsis leaf 18:3-Sitosterol 18:2-Sitosterol

13 Method Validation GC vs. Q-TOF MS GC Q-TOF Analysis of Arabidopsis WT leaves by GC and Q-TOF MS. Proof of concept Advantages of Q-TOF MS Only small amounts of plant tissue required Easy sample preparation allows for a large number of samples Direct measurement of conjugated sterols present at low abundances (esp. ASG)

14 Method Application Arabidopsis thaliana +P -P

15 Sterol Analysis in Arabidopsis under Phosphate Deprivation WT pho1 1, WT leaf (+P) 2, pho1 leaf (-P) + P -P 3, WT leaf +P 4, WT leaf P 5, WT root, +P 6, WT root, -P No major changes in the composition of sterol species. (here: free sterols, distribution similar in SE, SG and ASG)

16 Sterol Analysis in Arabidopsis under Phosphate Deprivation WT pho1 1, WT leaf (+P) 2, pho1 leaf (-P) * 3, WT leaf +P 4, WT leaf P 5, WT root, +P 6, WT root, -P P -P

17 Sterol Analysis in Arabidopsis under Phosphate Deprivation WT pho1 1, WT leaf (+P) 2, pho1 leaf (-P) * 3, WT leaf +P 4, WT leaf P 5, WT root, +P 6, WT root, -P P -P Sterol Ester amounts increase during P conditions, but also in plants grown in sterile culture: Sterol Ester accumulate during different stress conditions.

18 Sterol Analysis in Arabidopsis under Phosphate Deprivation WT pho1 1, WT leaf (+P) 2, pho1 leaf (-P) * 3, WT leaf +P 4, WT leaf P 5, WT root, +P 6, WT root, -P P -P Glycosylated sterols (SG, ASG) are increased during P conditions in leaves

19 Sterol Analysis in Arabidopsis under Phosphate Deprivation ASG WT pho1 1, WT leaf (+P) 2, pho1 leaf (-P) 3, WT leaf +P 4, WT leaf P 5, WT root, +P 6, WT root, -P + P -P 16:0-ASG decreased, and 18:3-ASG increased during Pi deprivation.

20 Conclusion Arabidopsis thaliana SG and ASG increased at Pi Glyosylated sterols replace phospholipids. (also see: Anderson et al JBC, Tjellström et al FASEB J) 18:3-ASG increased, 16:0-ASG decreased at - Pi

21 University of Bonn Institute Molecular Physiology Biotechnology of Plants (IMBIO) Instit te of Molec lar Ph siolog and Biotechnolog Vera Wewer Isabel Dombrink Katharina vom Dorp Peter Dörmann

Lipids Analysis. Lipids

Lipids Analysis. Lipids Lipids Analysis Stephen Barnes 3 5 15 Lipids Lipids are mostly very hydrophobic Most are conjugates of fatty acids of a variety of chain lengths, which have different degrees of unsaturation, cis trans

More information

Supplemental Figure S1

Supplemental Figure S1 Supplemental Figure S1 GC-MS profile of total FA of BY-2 purified PM IS: h14 IS: 17: Glycerolipids 16: GIPCs 18:1,2,3 18: h22 h24 Sterols GluCER h16: 2: h2 22: h21 c23 24: h23 h25 h26 Supplemental Figure

More information

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures Lipid Analysis Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th 2005 Introduction to lipid structures Fatty acids Acylglycerols Glycerophospholipids Sterols Strategies involved in lipid

More information

BIOCATALYTIC CONVERSION OF OTHER LIPIDS

BIOCATALYTIC CONVERSION OF OTHER LIPIDS Chapter 6 BIOCATALYTIC CONVERSION OF OTHER LIPIDS In Chapters 4 and 5, lipase-mediated conversion of acylglycerols were presented. The Chapter 6 deals with biocatalytic modification of phospholipids, sphingolipids,

More information

Molecular species composition of plant cardiolipin determined by liquid chromatography-mass

Molecular species composition of plant cardiolipin determined by liquid chromatography-mass Molecular species composition of plant cardiolipin determined by liquid chromatography-mass spectrometry Yonghong Zhou, Helga Peisker and Peter Dörmann Institute of Molecular Physiology and Biotechnology

More information

ANSC (NUTR) 618 LIPIDS & LIPID METABOLISM Membrane Lipids and Sphingolipidsd

ANSC (NUTR) 618 LIPIDS & LIPID METABOLISM Membrane Lipids and Sphingolipidsd ANSC (NUTR) 618 LIPIDS & LIPID METABOLISM Membrane Lipids and Sphingolipidsd I. Classes of membrane lipids A. Glycerolipids (quantitatively the most important of the three membrane lipids) B. Shingolipids

More information

Introduction to Proteomics 1.0

Introduction to Proteomics 1.0 Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using

More information

A mutant in Arabidopsis Lacking a Chloroplast Specific Lipid. Lewis Kurschner and Karen Thulasi Masters in Botany

A mutant in Arabidopsis Lacking a Chloroplast Specific Lipid. Lewis Kurschner and Karen Thulasi Masters in Botany A mutant in Arabidopsis Lacking a Chloroplast Specific Lipid Lewis Kurschner and Karen Thulasi Masters in Botany Fatty acid nomenclature Fatty acyl composition Chain length Degree of unsaturation and position

More information

Principles of Shotgun Lipidomics

Principles of Shotgun Lipidomics Principles of Shotgun Lipidomics Xianlin Han Diabetes and Obesity Research Center Sanford-Burnham Medical Research Institute Lake Nona Orlando, FL 32827 What is shotgun lipidomics? Original definition

More information

Chapter 2 Part 3: Organic and Inorganic Compounds

Chapter 2 Part 3: Organic and Inorganic Compounds Chapter 2 Part 3: Organic and Inorganic Compounds Objectives: 1) List the major groups of inorganic chemicals common in cells. 2) Describe the functions of various types of inorganic chemicals in cells.

More information

Gas Chromatography/ Mass Spectrometry

Gas Chromatography/ Mass Spectrometry Gas Chromatography/ Mass Spectrometry Edited by H.F. Linskens and J.F. Jackson Contributors R.S. Bandurski G. Combaut A.Ehmann P.Hedden B.Janistyn H.Kameoka H.Kodama D.V.Lynch J.K.MacLeod H.Nyberg L.M.S.Palni

More information

UC Davis The Proceedings of the International Plant Nutrition Colloquium XVI

UC Davis The Proceedings of the International Plant Nutrition Colloquium XVI UC Davis The Proceedings of the International Plant Nutrition Colloquium XVI Title The content of ß-sitosterol in maize plants growing under different nitrogen nutrition Permalink https://escholarship.org/uc/item/72j7v14x

More information

Structural organization of rapeseed oil bodies affects their stability and oil extractability

Structural organization of rapeseed oil bodies affects their stability and oil extractability Structural organization of rapeseed oil bodies affects their stability and oil extractability T Chardot, 2015-07-09 Saskatoon thierry,chardot@versailles,inra,fr http://www-ijpb.versailles.inra.fr/en/bs/equipes/biostructurale/index.htm

More information

Mass Spectrometry based metabolomics

Mass Spectrometry based metabolomics Mass Spectrometry based metabolomics Metabolomics- A realm of small molecules (

More information

Keywords: Cholesteryl ester; Plant sterol ester; Cholesterol esterase; Trichoderma sp. Stigmasterol

Keywords: Cholesteryl ester; Plant sterol ester; Cholesterol esterase; Trichoderma sp. Stigmasterol Novel process for enzymatic production of sterol esters in microaqueous solution with cholesterol esterase from Trichoderma sp. AS59 Atsushi Maeda a, Norihumi Hashitani a, Takayuki Mizuno a, Masanori Bunya

More information

The use of mass spectrometry in lipidomics. Outlines

The use of mass spectrometry in lipidomics. Outlines The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid

More information

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE

QUANTITATIVE ESTIMATION OF PHYTOSTEROL FROM TWO MEDICINALLY IMPORTANT PLANTS OF CUCURBITACEAE Int. J. Engg. Res. & Sci. & Tech. 2014 Renu Sarin and Sangeeta Samria, 2014 Research Paper ISSN 2319-5991 www.ijerst.com Vol. 3, No. 2, May 2014 2014 IJERST. All Rights Reserved QUANTITATIVE ESTIMATION

More information

Introduction to Lipid Chemistry

Introduction to Lipid Chemistry Introduction to Lipid Chemistry Benjamin Schwartz Ontario SCC Education Day September 18, 2018 Lipid knowledge for the personal care industry What is a Lipid? Lipids are fatty acids and their derivatives,

More information

Mass-Spectrometric Analysis of Lipids (Lipidomics)

Mass-Spectrometric Analysis of Lipids (Lipidomics) Mass-Spectrometric Analysis of Lipids (Lipidomics) 1. Identification 2. Quantification 3. Metabolism Why to do lipidomics? Biology: Functions of different lipids? Medicine: Diagnostics and Therapy Industry:

More information

The detergent-solubilized and gel filtration purified rhodopsin was partitioned against

The detergent-solubilized and gel filtration purified rhodopsin was partitioned against Supplement Jastrzebska et al. Materials and Methods The detergent-solubilized and gel filtration purified rhodopsin was partitioned against H 2 O/MeOH/CHCl 3, and the bottom layer was removed, dried down,

More information

Good Afternoon! 11/30/18

Good Afternoon! 11/30/18 Good Afternoon! 11/30/18 1. The term polar refers to a molecule that. A. Is cold B. Has two of the same charges C. Has two opposing charges D. Contains a hydrogen bond 2. Electrons on a water molecule

More information

Small Molecule Science: Experimental designs for achieving ultra trace analysis

Small Molecule Science: Experimental designs for achieving ultra trace analysis Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small

More information

CHANGES OF COMPOSITION IN TRIACYLGLYCEROLS, STEROLS AND TOCOPHEROLS OF FLAX DURING the VEGETATION

CHANGES OF COMPOSITION IN TRIACYLGLYCEROLS, STEROLS AND TOCOPHEROLS OF FLAX DURING the VEGETATION 112 Bulgarian Journal of Agricultural Science, 20 (No 1) 2014, 112-116 Agricultural Academy CHANGES OF COMPOSITION IN TRIACYLGLYCEROLS, STEROLS AND TOCOPHEROLS OF FLAX DURING the VEGETATION O. TENEVA 1,

More information

LC/MS Method for Comprehensive Analysis of Plasma Lipids

LC/MS Method for Comprehensive Analysis of Plasma Lipids Application Note omics LC/MS Method for Comprehensive Analysis of Plasma s Authors Tomas Cajka and Oliver Fiehn West Coast Metabolomics Center, University of California Davis, 451 Health Sciences Drive,

More information

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND LIPIDOMIC ANALYSIS Fourth Edition WILLIAM W.CHRISTIE MRS Lipid Analysis Unit, Scottish Crop Research Institute, Dundee, Scotland Invergowrie, and

More information

Lipids fatty, oily, or waxy hydrophobic organic compounds.

Lipids fatty, oily, or waxy hydrophobic organic compounds. Lipids Lipids Lipids fatty, oily, or waxy hydrophobic organic compounds. u long hydrocarbon chain u composed of CHO Diverse group u fats u oils u waxes u steroids Do not form polymers u big molecules made

More information

Lipids are used to store and excess energy from extra carbohydrates in animals

Lipids are used to store and excess energy from extra carbohydrates in animals Lipids Lipids are a major source of energy used by cells, however lipids are more difficult for your body to break down. They produce nearly twice the amount of energy than proteins or carbohydrates. Lipids

More information

OBJECTIVE. Lipids are largely hydrocarbon derivatives and thus represent

OBJECTIVE. Lipids are largely hydrocarbon derivatives and thus represent Paper 4. Biomolecules and their interactions Module 20: Saturated and unsaturated fatty acids, Nomenclature of fatty acids and Essential and non-essential fatty acids OBJECTIVE The main aim of this module

More information

Thermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS

Thermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS Thermo Scientific LipidSearch Software for Lipidomics Workflows Automated Identification and Relative of Lipids by LC/MS The promise of lipidomics Lipidomics is a new field of study crucial for understanding

More information

BIOB111_CHBIO - Tutorial activity for Session 12

BIOB111_CHBIO - Tutorial activity for Session 12 BIOB111_CHBIO - Tutorial activity for Session 12 General topic for week 6 Session 12 Lipids Useful Links: 1. Animations on Cholesterol (its synthesis, lifestyle factors, LDL) http://www.wiley.com/college/boyer/0470003790/animations/cholesterol/cholesterol.htm

More information

Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry. Jianzhong Chen, PhD Assistant Professor School of Optometry UAB

Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry. Jianzhong Chen, PhD Assistant Professor School of Optometry UAB Non targeted Lipidomic Analysis by Direct Infusion Mass Spectrometry Jianzhong Chen, PhD Assistant Professor School of Optometry UAB 1 Lipidome: A subset of Metabolome https://en.wikipedia.org/wiki/lipidomics

More information

Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson

Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson Link download full: http://testbankair.com/download/test-bank-forlehninger-principles-of-biochemistry-5th-edition-by-nelson/ Chapter

More information

IDENTIFICATION AND QUANTIFICATION OF LIPID METABOLITES IN COTTON FIBERS: RECONCILIATION WITH METABOLIC PATHWAY PREDICTIONS FROM DNA DATABASES.

IDENTIFICATION AND QUANTIFICATION OF LIPID METABOLITES IN COTTON FIBERS: RECONCILIATION WITH METABOLIC PATHWAY PREDICTIONS FROM DNA DATABASES. IDENTIFICATION AND QUANTIFICATION OF LIPID METABOLITES IN COTTON FIBERS: RECONCILIATION WITH METABOLIC PATHWAY PREDICTIONS FROM DNA DATABASES. Sylvia W. Wanjie, B.S. Thesis Prepared for the Degree of MASTER

More information

MASS SPECTROMETRY STRATEGIES FOR COMPREHENSIVE LIPIDOME ANALYSIS OF COLORECTAL CANCER CELLS AND THEIR SECRETED EXOSOMES. Cassie J.

MASS SPECTROMETRY STRATEGIES FOR COMPREHENSIVE LIPIDOME ANALYSIS OF COLORECTAL CANCER CELLS AND THEIR SECRETED EXOSOMES. Cassie J. MASS SPECTROMETRY STRATEGIES FOR COMPREHENSIVE LIPIDOME ANALYSIS OF COLORECTAL CANCER CELLS AND THEIR SECRETED EXOSOMES By Cassie J. Fhaner A DISSERTATION Submitted to Michigan State University in partial

More information

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids Jeremy Netto, 1 Stephen Wong, 1 Federico Torta, 2 Pradeep Narayanaswamy, 2

More information

Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer

Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer Application Note: 367 Essential Lipidomics Experiments using the LTQ rbitrap Hybrid Mass Spectrometer Thomas Moehring 1, Michaela Scigelova 2, Christer S. Ejsing 3, Dominik Schwudke 3, Andrej Shevchenko

More information

Dietary fat supplies essential body tissue needs, both as an energy fuel and a structural material.

Dietary fat supplies essential body tissue needs, both as an energy fuel and a structural material. Chapter 3 Fats Chapter 3 Lesson 3.1 Key Concepts Dietary fat supplies essential body tissue needs, both as an energy fuel and a structural material. Foods from animal and plant sources supply distinct

More information

Essential Components of Food

Essential Components of Food Essential Components of Food The elements of life living things are mostly (98%) made of 6 elements: C carbon H hydrogen O oxygen P phosphorus N nitrogen S sulphur -each element makes a specific number

More information

Impact of Chromatography on Lipid Profiling of Liver Tissue Extracts

Impact of Chromatography on Lipid Profiling of Liver Tissue Extracts Impact of Chromatography on Lipid Profiling of Liver Tissue Extracts Application Note Clinical Research Authors Mark Sartain and Theodore Sana Agilent Technologies, Inc. Santa Clara, California, USA Introduction

More information

Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry Meets a Key Challenge in Lipidomics

Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry Meets a Key Challenge in Lipidomics Metabolites 2011, 1, 21-40; doi:10.3390/metabo1010021 Review OPEN ACCESS metabolites ISSN 2218-1989 www.mdpi.com/journal/metabolites/ Accurate Quantification of Lipid Species by Electrospray Ionization

More information

Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry

Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry Raúl González-Domínguez * Department of Chemistry, Faculty of Experimental Sciences. University of Huelva, Spain. * Corresponding

More information

2. Simple lipids: Triacylglycerols and waxes are classified as simple lipids. The characteristics of each are described in the sections below.

2. Simple lipids: Triacylglycerols and waxes are classified as simple lipids. The characteristics of each are described in the sections below. Paper 4: Biomolecules and their interactions Module 21: Classification of Lipids: simple and compound lipids, phospholipids, Cholesterol OBJECTIVE The main aim of this module is to introduce the students

More information

Lipids are broadly classified in to simple, complex and derived, which are further subdivided into different groups.

Lipids are broadly classified in to simple, complex and derived, which are further subdivided into different groups. Paper No. 01 Paper Title: Food Chemistry Module -9: Classification of lipids Lipids are organic substances which are insoluble in water and soluble in organic solvents. Lipids are not polymers and exist

More information

3150:112 SAMPLE TEST 2. Print out a copy Answer the questions on your own. Check the answers at GOBC Ans.pdf. Good Luck!

3150:112 SAMPLE TEST 2. Print out a copy Answer the questions on your own. Check the answers at GOBC Ans.pdf. Good Luck! SAMPLE TEST 2 3150:112 Print out a copy Answer the questions on your own. Check the answers at GOBC Ans.pdf. Good Luck! QUESTIONS 1-3 REFER TO TE FOLLOWING: A. C 2 O O B. C 2 O O O C 2 O C. O C 2 O 1.

More information

Terminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids

Terminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Metabolome: the quantitative complement of all the low molecular weight molecules present in

More information

Lipididentifizierung in der LC-MS-basierten Lipidomik mittels einer Kombination aus SWATH und DMS

Lipididentifizierung in der LC-MS-basierten Lipidomik mittels einer Kombination aus SWATH und DMS Lipididentifizierung in der LC-MS-basierten Lipidomik mittels einer Kombination aus SWATH und DMS Michael Witting, et al. Helmholtz Zentrum München Research Unit Analytical BioGeoChemistry Berlin, 14/3/17

More information

Engineering wheat for take-all resistance. Aymeric Leveau & Emma Wallington

Engineering wheat for take-all resistance. Aymeric Leveau & Emma Wallington Engineering wheat for take-all resistance Aymeric Leveau & Emma Wallington Lab meeting 03-07-2013 Take-all disease of wheat Caused by the root-infecting pathogen Gaeumannomyces graminis var. tritici The

More information

Glycerolipidome responses to freezingand chilling-induced injuries: examples in Arabidopsis and rice

Glycerolipidome responses to freezingand chilling-induced injuries: examples in Arabidopsis and rice Zheng et al. BMC Plant Biology (2016) 16:70 DOI 10.1186/s12870-016-0758-8 RESEARCH ARTICLE Open Access Glycerolipidome responses to freezingand chilling-induced injuries: examples in Arabidopsis and rice

More information

Analysis of Lipid Metabolites. = Lipidomics

Analysis of Lipid Metabolites. = Lipidomics Analysis of Lipid Metabolites = Lipidomics Pentti Somerharju Transmed 11.10.2012 utline of the talk What is Lipidomics? Where is lipidomics needed? Lipid classes and their functions Why mass-spectrometric

More information

M ETHODS IN MOLECULAR BIOLOGY

M ETHODS IN MOLECULAR BIOLOGY M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651

More information

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document

More information

Yueming LU, Coralie BIACHE and R. Paul Philp

Yueming LU, Coralie BIACHE and R. Paul Philp Yueming LU, Coralie BIACHE and R. Paul Philp School of Geology & Geophysics, University of Oklahoma, Norman, OK7319, USA LIEC, University of Lorraine, Vandoeuvre-lès-Nancy, 5456, France. Session: Water

More information

Can you explain that monomers are smaller units from which larger molecules are made?

Can you explain that monomers are smaller units from which larger molecules are made? Biological molecules Can you explain that all living things have a similar biochemical basis? Can you explain that monomers are smaller units from which larger molecules are made? Can you describe polymers

More information

Anatomy & Physiology I. Macromolecules

Anatomy & Physiology I. Macromolecules Anatomy & Physiology I Macromolecules Many molecules in the human body are very large, consisting of hundreds or even thousands of atoms. These are called macromolecules. Four types of macromolecules are

More information

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016 LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry

More information

Very-Long Chain Fatty Acid Biosynthesis

Very-Long Chain Fatty Acid Biosynthesis Very-Long Chain Fatty Acid Biosynthesis Objectives: 1. Review information on the isolation of mutants deficient in VLCFA biosynthesis 2. Generate hypotheses to explain the absence of mutants with lesions

More information

Nafith Abu Tarboush DDS, MSc, PhD

Nafith Abu Tarboush DDS, MSc, PhD Nafith Abu Tarboush DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Lipids (cholesterol, cholesterol esters, phospholipids & triacylglycerols) combined with proteins (apolipoprotein) in

More information

Esters PAPER and Total Lipids Fractions from Cold Pressed Nonrefined Sesame and Walnut Oil

Esters PAPER and Total Lipids Fractions from Cold Pressed Nonrefined Sesame and Walnut Oil Fatty Acid Profile ORIGINAL PRELIMINARY of Phytosterol SCIENTIFIC COMMUNICATIONS Esters PAPER and Total Lipids Fractions from Cold Pressed Nonrefined Sesame and Walnut Oil Fatty Acid Profile of Phytosterol

More information

Relative Quantitation of Human Polymorphonuclear Leukocyte Cell Membrane GPEtn Lipids

Relative Quantitation of Human Polymorphonuclear Leukocyte Cell Membrane GPEtn Lipids Relative Quantitation of Human Polymorphonuclear Leukocyte Cell Membrane GPEtn Lipids Using the QTRAP System with mtraq Reagents Karin A. Zemski-Berry 1, John M. Hevko 2, and Robert C. Murphy 1 1 Department

More information

PHOSPHOLIPIDS METABOLISM. BY Dr. Walid Said Zaki Dr. Marwa Ali LECTURER OF BIOCHEMISTRY AND MOLECULAR BIOLOGY

PHOSPHOLIPIDS METABOLISM. BY Dr. Walid Said Zaki Dr. Marwa Ali LECTURER OF BIOCHEMISTRY AND MOLECULAR BIOLOGY PHOSPHOLIPIDS METABOLISM BY Dr. Walid Said Zaki Dr. Marwa Ali LECTURER OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 1. State the definition and classification of Phospholipids. 2. Describe the general structure

More information

Chapter 26 Biochemistry 5th edition. phospholipids. Sphingolipids. Cholesterol. db=books&itool=toolbar

Chapter 26 Biochemistry 5th edition. phospholipids. Sphingolipids. Cholesterol.   db=books&itool=toolbar http://www.ncbi.nlm.nih.gov/sites/entrez? db=books&itool=toolbar 1 The surface of a soap bubble is a bilayer formed by detergent molecules 2 Chapter 26 Biochemistry 5th edition phospholipids Sphingolipids

More information

Supporting Information

Supporting Information Supporting Information Supplemental Figure Legends Supplementary Figure 1. Experimental Design. A. To benchmark the performance of software parameter sets proteins were extracted from a pool of Arabidopsis

More information

* What are Sesqueterpens and Diterpenes *Their types. *How we can synthesis it? *Their biological activities *Their natural occurrence

* What are Sesqueterpens and Diterpenes *Their types. *How we can synthesis it? *Their biological activities *Their natural occurrence Terpenes * What are Sesqueterpens and Diterpenes *Their types. *How we can synthesis it? *Their biological activities *Their natural occurrence 3-Sesqueterpens 15 carbons, three isoprene units Types of

More information

LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell 120 EC C18 Column

LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell 120 EC C18 Column Application Note Clinical Research LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell EC C8 Column Authors Rongjie Fu and Zhiming Zhang Agilent Technologies

More information

Choosing the metabolomics platform

Choosing the metabolomics platform Choosing the metabolomics platform Stephen Barnes, PhD Department of Pharmacology & Toxicology University of Alabama at Birmingham sbarnes@uab.edu Challenges Unlike DNA, RNA and proteins, the metabolome

More information

High Performance Gas Chromatography Mass Spectrometry in Addressing the Challenges of Metabolomic Studies Separation in Time and Mass

High Performance Gas Chromatography Mass Spectrometry in Addressing the Challenges of Metabolomic Studies Separation in Time and Mass High Performance Gas Chromatography Mass Spectrometry in Addressing the Challenges of Metabolomic Studies Separation in Time and Mass Jeffrey S. Patrick Director of Marketed Technology LECO Separation

More information

Lipids. Lipids: a Diverse group of chemicals. Storage Lipids: derivatives of fatty acids. 11/21/10

Lipids. Lipids: a Diverse group of chemicals. Storage Lipids: derivatives of fatty acids. 11/21/10 1 Lipids Lehninger 3 rd ed. Chapter 11 (For biosynthesis see Chapter 21) 2 Lipids: a Diverse group of chemicals Insolubility in water. Fats and oils: energy stores. Phospholipids and sterols: structural

More information

Lipids: Membranes Testing Fluid Mosaic Model of Membrane Structure: Cellular Fusion

Lipids: Membranes Testing Fluid Mosaic Model of Membrane Structure: Cellular Fusion Models for Membrane Structure NEW MODEL (1972) Fluid Mosaic Model proposed by Singer & Nicholson Lipids form a viscous, twodimensional solvent into which proteins are inserted and integrated more or less

More information

A -GLS Arabidopsis Cuscuta gronovii

A -GLS Arabidopsis Cuscuta gronovii -GLS Arabidopsis Cuscuta gronovii internal standard 4MS Wildtype Arabidopsis Cuscuta gronovii base Cuscuta gronovii apex 3MSP internal standard MSP 4MT 8MSO 4MO 1MO Figure S1. Representative HPLC-DAD chromatograms

More information

4.1 Cycling of Matter Date: Cycling of Organic and Inorganic Matter. Build your Own Notes:

4.1 Cycling of Matter Date: Cycling of Organic and Inorganic Matter. Build your Own Notes: 4.1 Cycling of Matter Date: Build your Own Notes: Use these topics as guidelines to create your own notes for 4.1 from pages 83 84 Study Notes/Questions Cycling of Organic and Inorganic Matter Matter is

More information

Metabolomic and Proteomics Solutions for Integrated Biology. Christine Miller Omics Market Manager ASMS 2015

Metabolomic and Proteomics Solutions for Integrated Biology. Christine Miller Omics Market Manager ASMS 2015 Metabolomic and Proteomics Solutions for Integrated Biology Christine Miller Omics Market Manager ASMS 2015 Integrating Biological Analysis Using Pathways Protein A R HO R Protein B Protein X Identifies

More information

MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology

MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology Patrick R. Murray, PhD WW Director, Scientific Affairs BD Diagnostic Systems Outline MALDI-TOF is the most important innovation

More information

Synthesis and Solubility of Hydrophilic Derivatives of β- Sitosterol

Synthesis and Solubility of Hydrophilic Derivatives of β- Sitosterol J. Ind. Eng. Chem., Vol. 13, No. 3, (2007) 367-372 Synthesis and Solubility of Hydrophilic Derivatives of β- Sitosterol Dae-won Chung and Young Tai Choi Department of Polymer Engineering, College of Engineering,

More information

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID James Little, Eastman Chemical Company, Kingsport, TN Overview Phospholipids and

More information

Overview of the food science behind fatty acid technology

Overview of the food science behind fatty acid technology Overview of the food science behind fatty acid technology Pamela J. White, Ph.D. Food Chemist/Scientist Food Science and Human Nutrition Dept. & Center for Crops Utilization Research Iowa State University

More information

Agilent Solutions for Lipidomics. Greater Insight into

Agilent Solutions for Lipidomics. Greater Insight into Agilent Solutions for Lipidomics Greater Insight into Lipid Metabolism Understanding Lipidomics What is Lipidomics? The term "lipidome" refers to all the lipids that exist in an organism and their effects

More information

Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS

Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS Rapid Lipid Profiling of Serum by Reverse Phase UPLC-Tandem Quadrupole MS Mark Ritchie and Evelyn Goh Waters Pacific Pte Ltd., Singapore A P P L I C AT ION B E N E F I T S Delivers a rapid 10-min MRM method

More information

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19, MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents

More information

TEST BANK FOR LEHNINGER PRINCIPLES OF BIOCHEMISTRY 6TH EDITION BY NELSON

TEST BANK FOR LEHNINGER PRINCIPLES OF BIOCHEMISTRY 6TH EDITION BY NELSON Link full download: https://testbankservice.com/download/testbank-for-lehninger-principles-of-biochemistry-6th-edition-bynelson TEST BANK FOR LEHNINGER PRINCIPLES OF BIOCHEMISTRY 6TH EDITION BY NELSON

More information

Separation of Campesterol and b-sitosterol from a Sterol Mixture

Separation of Campesterol and b-sitosterol from a Sterol Mixture Separation Science and Technology, 41: 3027 3038, 2006 Copyright # Taylor & Francis Group, LLC ISSN 0149-6395 print/1520-5754 online DOI: 10.1080/01496390600851699 Separation of Campesterol and b-sitosterol

More information

Cell Membrane: a Phospholipid Bilayer. Membrane Structure and Function. Fluid Mosaic Model. Chapter 5

Cell Membrane: a Phospholipid Bilayer. Membrane Structure and Function. Fluid Mosaic Model. Chapter 5 Membrane Structure and Function Chapter 5 Cell Membrane: a Phospholipid Bilayer Phospholipid Hydrophilic Head Hydrophobic Tail Lipid Bilayer Fluid Mosaic Model Mixture of saturated and unsaturated fatty

More information

Fatty Acid Methylation Kits

Fatty Acid Methylation Kits Methyl esterification kit for fatty acids analysis Fatty Acid Methylation Kits Below are two methods for efficiently preparing fatty acid samples for GC analysis. Neither method requires high temperatures,

More information

The phosphate group replaces the fatty acid on C number 3 of a triacylglycerol molecule O O CH 2 O C R CH 2 O P O X OH.

The phosphate group replaces the fatty acid on C number 3 of a triacylglycerol molecule O O CH 2 O C R CH 2 O P O X OH. Phosphoacylglycerols (Phospholipids) Phosphoacylglycerols are fatty acid esters of glycerol which also contain a phosphate group and other specific groups The phosphate group replaces the fatty acid on

More information

Very-Long Chain Fatty Acid Biosynthesis

Very-Long Chain Fatty Acid Biosynthesis Very-Long Chain Fatty Acid Biosynthesis Objectives: 1. Review information on the isolation of mutants deficient in VLCFA biosynthesis 2. Generate hypotheses to explain the absence of mutants with lesions

More information

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010 MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited

More information

Automated Lipid Identification Using UPLC/HDMS E in Combination with SimLipid

Automated Lipid Identification Using UPLC/HDMS E in Combination with SimLipid Automated Lipid Identification Using UPLC/HDMS E in Combination with SimLipid Giorgis Isaac, Stephen McDonald, Giuseppe Astarita Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S

More information

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS LC/MS/MS SOLUTIONS FOR LIPIDOMICS Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS Lipids play a key role in many biological processes, such as the formation of cell membranes and signaling

More information

Improving Coverage of the Plasma Lipidome Using Iterative MS/MS Data Acquisition Combined with Lipid Annotator Software and 6546 LC/Q-TOF

Improving Coverage of the Plasma Lipidome Using Iterative MS/MS Data Acquisition Combined with Lipid Annotator Software and 6546 LC/Q-TOF Application Note Lipidomics Improving Coverage of the Plasma Lipidome Using Iterative MS/MS Data Acquisition Combined with Lipid Annotator Software and 6546 LC/Q-TOF Authors Jeremy Koelmel Department of

More information

Section 1 Lecture 1- Origins of Life Life probably started by Hydrothermal Vents.

Section 1 Lecture 1- Origins of Life Life probably started by Hydrothermal Vents. Section 1 Lecture 1- Origins of Life Life probably started by Hydrothermal Vents. Photosynthesis originated around 3GA, as cells figured out how to fix CO2 and release O2. Eukaryotes originates 1.5-2.5

More information

Lipids and Classification:

Lipids and Classification: Lipids and Classification: Lipids: Biological lipids are a chemically diverse group of organic compounds which are insoluble or only poorly soluble in water. They are readily soluble in non-polar solvents

More information

Chem Lecture 8 Lipids and Cell Membranes

Chem Lecture 8 Lipids and Cell Membranes Chem 452 - Lecture 8 Lipids and Cell Membranes 111114 Like carbohydrates, lipids are one of the four major classes of biomolecules, which also include the proteins, carbohydrates and nucleic acids. Lipids

More information

Very-Long Chain Fatty Acid Biosynthesis

Very-Long Chain Fatty Acid Biosynthesis Very-Long Chain Fatty Acid Biosynthesis Objectives: 1. Review information on the isolation of mutants deficient in VLCFA biosynthesis 2. Generate hypotheses to explain the absence of mutants with lesions

More information

Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley

Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley This protocol describes the extraction and direct measurement of cholesterol esters (CEs) and triacylglycerols

More information

Will s Pre-Test. (4) A collection of cells that work together to perform a function is termed a(n): a) Organelle b) Organ c) Cell d) Tissue e) Prison

Will s Pre-Test. (4) A collection of cells that work together to perform a function is termed a(n): a) Organelle b) Organ c) Cell d) Tissue e) Prison Will s Pre-Test This is a representative of Exam I that you will take Tuesday September 18, 2007. The actual exam will be 50 multiple choice questions. (1) The basic structural and functional unit of the

More information

PHYTOSTEROLS BIOLOGICAL ACTIVE COMPOUNDS IN FOOD. Abstract

PHYTOSTEROLS BIOLOGICAL ACTIVE COMPOUNDS IN FOOD. Abstract S.L. Pascal, et all. Journal of Agroalimentary Processes and Technologies, Volume XII, No. 1 (2006), 149-158 Review - Natural Food Extracts and Additives Section PHYTOSTEROLS BIOLOGICAL ACTIVE COMPOUNDS

More information

Kazusa DNA Res Inst. Daisuke Shibata

Kazusa DNA Res Inst. Daisuke Shibata Workshop Argentina-Japan Bioscience and Biotechnology for the promotion of Agricutulure and Food Production August 3 rd to 7 th 2009- Metabolite Metabolomics Databases approaches for Plant for Agro-Biotechnology

More information

Biomolecules. Unit 3

Biomolecules. Unit 3 Biomolecules Unit 3 Atoms Elements Compounds Periodic Table What are biomolecules? Monomers vs Polymers Carbohydrates Lipids Proteins Nucleic Acids Minerals Vitamins Enzymes Triglycerides Chemical Reactions

More information

Chapter 5: The Structure and Function of Large Biological Molecules

Chapter 5: The Structure and Function of Large Biological Molecules Chapter 5: The Structure and Function of Large Biological Molecules 1. Name the four main classes of organic molecules found in all living things. Which of the four are classified as macromolecules. Define

More information

Summary of fatty acid synthesis

Summary of fatty acid synthesis Lipid Metabolism, part 2 1 Summary of fatty acid synthesis 8 acetyl CoA + 14 NADPH + 14 H+ + 7 ATP palmitic acid (16:0) + 8 CoA + 14 NADP + + 7 ADP + 7 Pi + 7 H20 1. The major suppliers of NADPH for fatty

More information

Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease

Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease Comprehensive Lipid Profiling of Human Liver Tissue Extracts of Non-Alcoholic Fatty Liver Disease Multiplexed Precursor Ion Scanning and LipidView Software Brigitte Simons 1 and Bianca Arendt 2 1 AB SCIEX,

More information