Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Ionization source. Mass analyzer. Detector.

Size: px
Start display at page:

Download "Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Ionization source. Mass analyzer. Detector."

Transcription

1 Mass Analyzers Árpád Somogyi OSU, Summer Workshop August 18, 2015 Want to do MS or MS/MS? Need a Mass Spectrometer Ionization source Mass analyzer Detector inlet all ions sorted ions Data system 1

2 Ionization source Mass analyzer Detector inlet all ions sorted ions Data system Ion movement Biased metal plates (electrodes, lenses) used to move ions between ion source and analyzers Electrodes and physical slits used to shape and restrict ion beam Good sensitivity is dependent on good ion transmittance efficiency in this area Ionization source Mass analyzer Detector inlet all ions sorted ions Data system 2

3 Ion detection Ions can be detected efficiently w/ high amplification by accelerating them into surfaces that eject electrons Ionization source Mass analyzer Detector inlet all ions sorted ions Data system Ion energy Kinetic energy of ions defined by E = zev = qv = ½ mv 2 E = kinetic energy m = mass v = velocity e = electronic charge ( e -19 C) z = nominal charge V = accelerating voltage 3

4 Learning Check Consider two electrodes, one at 1000 V and one at ground (0 V) 1000 V 0 V + ion will travel with kinetic energy of Question: Consider an ion source block and an extraction lens. How would you bias the block and lens if you want the ions to be accelerated by a) 8000 ev (appropriate for magnetic sector) b) 5 ev (appropriate for entering a quadrupole) c) 20,000 ev (appropriate for entering a TOF) 4

5 Mass Resolution Mass spectrometers separate ions with a defined resolution/resolving power Resolving power- the ability of a mass spectrometer to separate ions with different mass to charge (m/z) ratios. Resolution defined at 10% valley M R=M/ M M 5

6 Example of ultrahigh resolution in an FTICR J. Throck Watson Introduction to Mass Spectrometry p. 103 General about mass spectrometers Common features Accelerated charged species (ions) interact with/can be controlled by Electrostatic field (ESA, OT) Magnetic field (B, ICR) Electromagnetic (rf) fields (Q, IT, LT) Or ions just fly (TOF) 6

7 For each of the following applications, choose the most appropriate mass analyzer from the following list. orbitrap (OT) time-of-flight (TOF) quadrupole (Q) FTICR Analyzer Purpose synthetic organic chemist wants exact mass of compound biochemist wants protein molecular weight of relatively large protein (MW 300,000) EPA (Environmental Protection Agency) wants confirmation of benzene in extracts from 3000 soil samples Petroleum chemist wants to confirm the presence of 55 unique compounds at one nominal mass/charge value in a mass spectrum Desirable mass spectrometer characteristics 7

8 Desirable mass spectrometer characteristics 1) They should sort ions by m/z 2) They should have good transmission (improves sensitivity) 3) They should have appropriate resolution (helps selectivity) 4) They should have appropriate upper m/z limit 5) They should be compatible with source output (pulsed or continuous) Mass spectrometers record m/z values m/z values are determined by actually measuring different physical parameters Type of analyzer electric sector magnetic sector quadrupole, ion trap time-of-flight FT-ion cyclotron resonance Physical parameter used as basis for separation kinetic energy/z momentum/z m/z flight time m/z (resonance frequencies) 8

9 Types of Mass Analyzers Magnetic (B) and/or Electrostatic (E) (HISTORIC/OLDEST) Time-of-flight (TOF) Quadrupole (Q) Quadrupole Ion Trap (IT) Linear Ion Trap (LT) Orbitrap Fourier Transform-Ion Cyclotron Resonance (ICR) Performance Advantages / Disadvantages / $$$ TOF Quadrupole Quadrupole Ion trap FTICR Orbitrap FTICR 9

10 Notice: accelerating voltages vary with analyzer (has consequences for MS/MS) High voltage (kev energy range) magnet (B) electrostatic (E) time-of-flight (TOF) Low voltage (ev energy range) quadrupole (Q) ion trap (IT, LT) ion cyclotron resonance (ICR) MS and MS/MS revisited Ionization Analysis MS: Collide with target to produce fragments MS/MS: Ionization Selection Activation Analysis 10

11 Current popular MS/MS arrangements Tandem in Space QqQ Q Trap Q TOF TOF TOF LTQ-Orbi (& QExactive) Tandem in Time Ion Trap (2 or 3 D) FT-ICR (FT) Decision factors when choosing a mass spectrometer S p e e e e e e e e e e e e e d R e s o l u t i o n Sensitivity Dynamic range Co$t 11

12 TOF Quadrupole Quadrupole Ion trap Orbitrap FTICR Time of Flight D m/z Detector V KE = zev = ½mv 2 v = D/t ½m(D/t) 2 = zev t = m 1 / 2 2zeV D m = mass V = velocity D = distance of flight t = time of flight KE = kinetic energy e = charge 12

13 How does the ion generation step in TOF influence m/z analysis? Consider MALDI h Target + Matrix Analyte Analyte ion may have (1) Kinetic Energy distribution or (2) Spatial distribution How will KE spread influence the spectrum? Ions of same m/z Has slightly greater KE Effect is broad peaks c/o Cotter 13

14 How will KE spread influence the spectrum? Solution to peak broadening caused by kinetic energy spread: Reflectron (ion mirror) Series of ring electrodes, typically with linear voltage gradient Time-of-Flight Reflectron Increased resolution by compensating for KE spread from the source 14

15 Reflectron TOF Mass Analyzer (TOF) Detector Reflectron Ion Source (MALDI) x x x x x High resolution KE = ½ mv 2 Reflectron TOF Mass Analyzer (TOF) Detector Reflectron Ion Source (MALDI) x High resolution KE = ½ mv 2 15

16 We talked about how to deal with kinetic energy spread. How do we deal with ions formed at different locations in the source (spatial distribution)? 16

17 Delayed Extraction 10 KV 10 KV KV Low mass (below 40 k Da) High resolution Continuous vs. Delayed Extraction Continuous TOF Resolution = 700 (FWHM) Delayed Extraction Resolution = 6000 (FWHM) 17

18 Recent TOF designs: improved resolution, better sensitivity and mass accuracy (Ultraflex III MALDI TOF-TOF) In te n s. [a.u.] Resolution: 25,000 S/N: 46 * Pepmix\0_N6\1\1SRef m/z Typical TOF Specs m/z Range: unlimited u Resolution: ~20,000 (Reflectron) Mass Accuracy: ~ 3-10 ppm (300-4,000 u) Scan Speed: 10 6 u/s Vacuum: 10-7 Torr Quantification: low - medium Positive and Negative Ions Variations: Linear, Reflectron, Tandem: w/ quads, TOFs and/or sectors 18

19 TOF Quad 3D Trap Linear Trap FT ICR Orbitrap mass range Resolution exact m/z sensitivity all ion detection ion storage speed dynamic range quantification MS/MS types sample introduction simplicity cost/performance Can an MS/MS instrument be constructed if the only analyzer type available is TOF? 19

20 TOF-TOF High-energy (kev) Collisions with Gas in a TOF-TOF 20

21 16 O/ 18 O-labeled DLEEGIQTLMGR *Resolution Medzihradszky, KF et al. Anal Chem. 2000, 72: High (kev) collisions allow for w peptide ions and immonium ions Intens. [a.u.] x m/z MALDI TOF-TOF fragmentation spectrum of a sodiated polymer O O O CH 2 CH 2 * m 3-OEB, m=1-3 (164, 44) Copolymer of 2-hydroxybenzoic acid and ethylene carbonate n 21

22 Advantages and Disadvantages Mass Spectrometer Advantages Disadvantages TOF-TOF high resolution, high Large size m/z fragment ions kev CID Not ideal for continuous ionization source easier de novo peptide sequencing d n and w n to distinguish Ile/Leu $$$ MALDI source Analyzers TOF Quadrupole Quadrupole Ion trap Orbitrap FTICR 22

23 Quadrupole (Q) 23

24 Quadrupole (Q) four parallel rods or poles fixed DC and alternating RF voltages rf voltage +dc voltage rf voltage 180 out of phase -dc voltage only particular m/z will be focused on the detector, all the other ions will be deflected into the rods scan by varying the amplitude of the voltages (AC/DC constant). Quadrupole Field Animation Negative rod Positive rod Positive rod Negative rod 24

25 negative DC offset -DC positive DC offset + +DC Ion Motion in Quadrupoles - a qualitative understanding + DC, ions focused to center - DC, ions defocused +DC w/ rf, light ions respond to rf, eliminated (high mass filter) -DC w/ rf, light ions respond to rf, focused to center (low mass filter) 25

26 Quadrupole animation Initial kinetic energy affects ion motion in quad 26

27 Figure 9. The a-q stability diagram: a) The shaded area represents those areas in a-q space which correspond to stable solutions of Mathieu s differential equation. B) The one amu bandpass mass filter: Notice that only ions of m/e m+1 fall within the stability diagram. Miller, P., Denton, M.B., J.Chem Ed., 63:7, pg 617, Quadrupole Typical Specs m/z Range: u Resolution: Unit Mass Accuracy: ca +/- 0.1 u Scan Speed: 4000 u/s Vacuum: Torr Low Voltages: RF ~ V DC ~500V-840V Source near ground Quantification: good choice Positive and Negative Ions Variations: SingleQ, TripleQ, Hybrids 27

28 TOF Quad 3D Trap Linear Trap FT ICR Orbitrap mass range Resolution exact m/z sensitivity all ion detection ion storage speed dynamic range quantification MS/MS types sample introduction simplicity cost/performance What MS/MS instruments can be produced from Q? What MS/MS instruments can be produced from Q and TOF? 28

29 Triple Quadrupole - since 1970 s and still going strong! Q1 Q2 Q3 S D Attractive Features: Source near ground and operates at relatively high pressure Couples well to source and to chromatography Multiple scan modes easy to implement Triple Quadrupole (QQQ) Detection System Dynode Turbo Q3 Source Q0 Q1 Q3 Q2 Q2 Heated Capillary Q1 Q0 Q00 c/o Thermo Finnigan Corp. c/o Agilent Corp. 29

30 Quadrupole Time of Flight (Q-TOF) (outdated) Low-energy (ev) Collisions with Gas 30

31 Q-TOF 80 fmol BSA digest QQQ Shevchenko, A., et al., Rapid. Commum. Mass Spectrom., 1997, 11: Analyzers TOF Quadrupole Quadrupole Ion trap Orbitrap FTICR 31

32 Linear Trap Demo Ion Transfer Tube Skimmer Tube Lens TOF Quad 3D Trap Linear Trap FT ICR Orbitrap mass range Resolution exact m/z sensitivity all ion detection ion storage speed dynamic range quantification MS/MS types sample introduction simplicity cost/performance 32

33 Suggested Reading List GENERAL MS AND MS/MS Kinter, M. and Sherman, N. E., Protein Sequencing and Identification Using Tandem Mass Spectrometry, Wiley and Sons, New York, NY, De Hoffmann E., Mass Spectrometry Principles and Applications (Second Edition), Wiley and Sons, New York, NY, Busch, K.L., et al., Mass spectrometry/ mass spectrometry: techniques and applications of tandem mass spectrometry, VCH Publishing, New York, NY, McLafferty, F.W. (Ed) Tandem Mass Spectrometry, Wiley and Sons, New York, NY, McLafferty, F.W. and Turecek, F. Interpretation of Mass Spectra, University Science Books, New York, NY, Siuzdak, G. Mass Spectrometry for Biotechnology, Academic Press, San Diego, CA, Gygi, S.P. and Aebersold, R., Curr. Opin. Chem. Biol., 4 (5): 489, Roepstorff, P., Curr. Opin. Biotech., 8: 6, De Hoffmann, JMS, 31: 129, Mann, M. et al., Annu. Rev. Biochem., 70: 437, McLuckey, S and Wells, J.M., Chem. Rev., 101: 571, Suggested Reading List Q-TOF Morris, H.R. et al., J. Protein Chem., 16: 469, Shevchencko A., et al., Rapid Commun. Mass Spectrom., 11: 1015, Shevchencko A., et al., Anal. Chem., 72: 2132, Medzihradszky, K.F., et al., Anal. Chem., 72: 552, Glish, G.L. and Goeringer, D.E., Anal. Chem., 56: 2291, 1984 Morris, H.R. et al., Rapid Commun. Mass Spectrom., 10: 889, Wattenberg, A. et al., JASMS, 13 (7): 772, Lododa, A.V. et al., Rapid Commun. Mass Spectrom., 14(12): 1047, TOF Bush, K., Spectroscopy, 12: 22, Cotter, R.J., Time-of-Flight: Instrumentation and Applications in Biological Research, ACS, Washington, DC,

34 Suggested Reading List TOF-TOF Yergey, A.L. et al., JASMS, 13 (7): 784, Go, E.P. et al., Anal. Chem., 75: 2504, FTICR Buchanan, M.V. (Ed), Fourier Transform MS, ACS, Washington, DC, Comisarow, M.B. and Marshall, A. G., Chem. Phys. Lett., 25: 282, McIver, R.T. et al., Int. J. Mass Spectrom. Ion Processes, 64: 67, Kofel, P. et al., Int. J. Mass Spectrom. Ion Processes, 65: 97, Williams, E. R. Anal. Chem., 70: 179A, McLafferty, F. W. Acc. Chem. Res., 27: 379, Fridriksson, E. K. et al., Biochemistry, 39: 3369, Fridriksson, E. K. et al., Biochemistry, 38: 8056, Kelleher, N. L.et al., Protein Science, 7: 1796, McLafferty, F. W. et al., Int. J. Mass Spectrom., 165: 457, Suggested Reading List Green, M. K. and Lebrilla, C. B. Mass Spectrom. Rev., 16: 53, Guan, S. and Marshall, A. G. Chem. Rev., 94: 2161, 1994 QIT Marsh, R.E. et al., Quadrupole Storage Mass Spectrometry, vol.102, Wiley and Sons, New York, NY, Cooks, R.G. et al., Ion trap mass spectrometry. Chem. Eng. News, 69(12): 26, Jonscher, K.R. et al., Anal Biochem, 244(1): 1, Louris, J.N. et al., Anal. Chem., 59(13): 1677, Louris, J.N. et al., Int. J. Mass Spectrom. Ion Processes, 96(2): Cooks, R.G. et al., Int. J. Mass Spectrom. Ion Processes, : McLuckey, S. A. et al., Int. J. Mass Spectrom. Ion Processes, 106: 213, Ngoka, L. C. M. and Gross, M. L. Int. J. Mass Spectrom. 194: 247,

35 Suggested Reading List QQQ Yost, R. A. and Enke, C. G. J. Am. Chem. Soc., 100: 2274, Arnott, D. in Proteome Research: Mass Spectrometry, james, P (Ed.), pp 11-31, Springer-Verlag, Germany, Steen H. et al., JMS, 36: 782, 2001 Miller, P.E. and Denton, M.B., J. Chem. Educ., 7: 617, Yang, l. et al., Rapid Commun. Mass Spectrom., 16(21): 2060, Mohammed, S. et al., JMS, 36: 1260, Dongre, A.R. et al., JMS, 31: 339, Orbitrap Hu, Q, Noll, RJ, Li, H., Makarov, A., Hardman, M., Cooks, R.G. JMS, , Makarov A. Anal. Chem. 2000; 72(6): Makarov A, Denisov E, Kholomeev A, Baischun W, Lange O, Strupat K, Anal. Chem. 2006; 78(7): Makarov A, Denisov E, Lange O, Horning S. JASMS. 2006; 17(7): Macek B, Waanders LF, Olsen JV, Mann M. Mol. Cell. Proteom. 2006; 5(5): Perry RH, Cooks RG, Noll RJ. Mass Spectrom. Rev. 2008; 27(6): Scigelova M, Makarov A. Orbitrap mass analyzer - Overview and applications in proteomics. Proteomics. 2006: Olsen JV, Macek B, Lange O, Makarov A, Horning S, Mann M. Nature Methods. 2007; 4(9):

Mass Analyzers. Árpád Somogyi. Campus Chemical Instrument Center Ohio State University. OSU, July 13, What is Mass Spectrometry?

Mass Analyzers. Árpád Somogyi. Campus Chemical Instrument Center Ohio State University. OSU, July 13, What is Mass Spectrometry? Mass Analyzers Árpád Somogyi Campus Chemical Instrument Center Ohio State University OSU, July 13, 2016 What is Mass Spectrometry? Mass spectrometry is a powerful tool in analytical/bioanalytical chemistry

More information

Mass Analyzers. Ion movement

Mass Analyzers. Ion movement Mass Analyzers Árpád Somogyi CCIC MSP OSU Summer Workshop Ion movement Biased metal plates (electrodes, lenses) used to move ions between ion source and analyzers Electrodes and physical slits used to

More information

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Summer Workshop. Mass analyzer. Ionization source.

Mass Analyzers. Want to do MS or MS/MS? Need a Mass Spectrometer. Árpád Somogyi. Summer Workshop. Mass analyzer. Ionization source. Mass Analyzers Árpád Somogyi Summer Workshop Want to do MS or MS/MS? Need a Mass Spectrometer Ionization source Mass analyzer Detector inlet all ions sorted ions Data system 1 Ionization source Mass analyzer

More information

FOURIER TRANSFORM MASS SPECTROMETRY

FOURIER TRANSFORM MASS SPECTROMETRY FOURIER TRANSFORM MASS SPECTROMETRY https://goo.gl/vx3ogw FT-ICR Theory Ion Cyclotron Motion Inward directed Lorentz force causes ions to move in circular orbits about the magnetic field axis Alan G. Marshall,

More information

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according

More information

Ion Source. Mass Analyzer. Detector. intensity. mass/charge

Ion Source. Mass Analyzer. Detector. intensity. mass/charge Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS

More information

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods

More information

Lecture 3. Tandem MS & Protein Sequencing

Lecture 3. Tandem MS & Protein Sequencing Lecture 3 Tandem MS & Protein Sequencing Nancy Allbritton, M.D., Ph.D. Department of Physiology & Biophysics 824-9137 (office) nlallbri@uci.edu Office- Rm D349 Medical Science D Bldg. Tandem MS Steps:

More information

1. Sample Introduction to MS Systems:

1. Sample Introduction to MS Systems: MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:

More information

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique

More information

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its

More information

Comparison of mass spectrometers performances

Comparison of mass spectrometers performances Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion

More information

FOURIER TRANSFORM MASS SPECTROMETRY

FOURIER TRANSFORM MASS SPECTROMETRY FOURIER TRANSFORM MASS SPECTROMETRY FT-ICR Theory Ion Cyclotron Motion Inward directed Lorentz force causes ions to move in circular orbits about the magnetic field axis Alan G. Marshall, Christopher L.

More information

FOURIER TRANSFORM MASS SPECTROMETRY

FOURIER TRANSFORM MASS SPECTROMETRY FOURIER TRANSFORM MASS SPECTROMETRY FT-ICR Theory Ion Cyclotron Motion Inward directed Lorentz force causes ions to move in circular orbits about the magnetic field axis Alan G. Marshall, Christopher L.

More information

Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications

Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications Considerations of the use of Triple Quadrupoles or Ion Traps in Quantitative Applications Triple Stage Quadrupole API MS / MS Full Scan Products / - IONS AND NEUTRALS FORMED IN API SOURCE Q0 LENS TRANSPORTS

More information

Time (min) Supplementary Figure 1: Gas decomposition products of irradiated DMC.

Time (min) Supplementary Figure 1: Gas decomposition products of irradiated DMC. 200000 C 2 CH 3 CH 3 DMC 180000 160000 140000 Intensity 120000 100000 80000 60000 40000 C 2 H 6 CH 3 CH 2 CH 3 CH 3 CCH 3 EMC DEC 20000 C 3 H 8 HCCH 3 5 10 15 20 25 Time (min) Supplementary Figure 1: Gas

More information

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE

More information

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Content Lectures 1-3 The basics of mass measurement Ionisation techniques Mass analysers Detectors

More information

MS/MS Scan Modes. Eötvös University, Budapest April 16, MS/MS Scan Modes. Árpád Somogyi. Product Ion Scan Select. Scan. Precursor Ion Scan Scan

MS/MS Scan Modes. Eötvös University, Budapest April 16, MS/MS Scan Modes. Árpád Somogyi. Product Ion Scan Select. Scan. Precursor Ion Scan Scan MS/MS Modes Árpád Somogyi Eötvös University, Budapest April 16, 2012 MS/MS Modes Product Ion Precursor Ion Neutral Loss Δ ed Reaction Monitoring (SRM) 1 modes in a triple quadrupole (QqQ) (one quadrupole

More information

MALDI-TOF. Introduction. Schematic and Theory of MALDI

MALDI-TOF. Introduction. Schematic and Theory of MALDI MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein

More information

Mass Spectrometry Infrastructure

Mass Spectrometry Infrastructure Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical

More information

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample

More information

New Instruments and Services

New Instruments and Services New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo

More information

Fundamentals of Soft Ionization and MS Instrumentation

Fundamentals of Soft Ionization and MS Instrumentation Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:

More information

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations For Research Use Only. Not for use in diagnostic procedures. 1 2015 AB Sciex RUO-MKT-11-1018-A For research

More information

Chapter 12: Mass Spectrometry: molecular weight of the sample

Chapter 12: Mass Spectrometry: molecular weight of the sample Structure Determination: hapter 12: Mass Spectrometry- molecular weight of the sample; formula hapter 12: Infrared Spectroscopy- indicated which functional groups are present hapter 13: Nuclear Magnetic

More information

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction

More information

(III) MALDI instrumentation

(III) MALDI instrumentation Dr. Sanjeeva Srivastava (I) Basics of MALDI-TF (II) Sample preparation In-gel digestion Zip-tip sample clean-up Matrix and sample plating (III) MALDI instrumentation 2 1 (I) Basics of MALDI-TF Analyte

More information

Mass Spectrometry Introduction

Mass Spectrometry Introduction Mass Spectrometry Introduction Chem 744 Spring 2013 What MS is and is not MS is NOT a spectroscopic method. Molecules are not absorbing EM radiation MS is the generation, separation and characterization

More information

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism

More information

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems

More information

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100

More information

More structural information with MS n

More structural information with MS n PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with

More information

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics

New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics July.3.13 Ken Miller Vice President of Marketing, Life Sciences Mass Spectrometry 1 The world leader in serving science Omics & the

More information

Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer

Essential Lipidomics Experiments using the LTQ Orbitrap Hybrid Mass Spectrometer Application Note: 367 Essential Lipidomics Experiments using the LTQ rbitrap Hybrid Mass Spectrometer Thomas Moehring 1, Michaela Scigelova 2, Christer S. Ejsing 3, Dominik Schwudke 3, Andrej Shevchenko

More information

SYNAPT G2-S High Definition MS (HDMS) System

SYNAPT G2-S High Definition MS (HDMS) System SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business

More information

SCS Mass Spectrometry Laboratory

SCS Mass Spectrometry Laboratory SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training

More information

New Instruments and Services

New Instruments and Services New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion

More information

Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules

Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Add a New Dimension to your Research Capability with Agilent s New Drift Ion Mobility Q-TOF System Overview: 6560 IM

More information

Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University

Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University Mass Spectrometry at the Wageningen University Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC-

More information

Since its invention in the 1980s, a matrix-assisted laser

Since its invention in the 1980s, a matrix-assisted laser FOCUS: THE ORBITRAP MALDI Produced Ions Inspected with a Linear Ion Trap-Orbitrap Hybrid Mass Analyzer Kerstin Strupat, a, * Viatcheslav Kovtoun, b, * Huy Bui, b Rosa Viner, b George Stafford, b and Stevan

More information

Solving practical problems. Maria Kuhtinskaja

Solving practical problems. Maria Kuhtinskaja Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements

More information

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance Bruker Daltonics Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance The new ultraflextreme exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary khz smartbeam-ii TM MALDI

More information

Agilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value

Agilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value Agilent 64 Triple Quadrupole LC/MS Sensitivity, Reliability, Value Sensitivity, Reliability, Value Whether you quantitate drug metabolites, measure herbicide levels in food, or determine contaminant levels

More information

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS

LC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS LC/MS/MS SOLUTIONS FOR LIPIDOMICS Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS Lipids play a key role in many biological processes, such as the formation of cell membranes and signaling

More information

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Kai Scheffler, PhD BioPharma Support Expert,LSMS Europe The world

More information

Biological Mass Spectrometry. April 30, 2014

Biological Mass Spectrometry. April 30, 2014 Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing

More information

Orbitrap technology update

Orbitrap technology update Orbitrap technology update Users Meeting Somerset, Oct 12, 211 Dr. Thomas Moehring Outline Users Meeting 21 Technology Update 211 - Orbitrap Elite - Q Exactive Summary 2 Your Goal: The ideal mass analyzer

More information

Agilent s LC/MS Portfolio and Applications Examples

Agilent s LC/MS Portfolio and Applications Examples Agilent s LC/MS Portfolio and Applications Examples Sean Orlowicz LC/MS Product Specialist Agilent Technologies 31August Agilent s > 40 year heritage in mass spectrometry 1971, 5930A GC/MS 1971-2011 2005,

More information

Advances in Hybrid Mass Spectrometry

Advances in Hybrid Mass Spectrometry The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap

More information

Welcome! Mass Spectrometry meets Cheminformatics WCMC Metabolomics Course 2014 Tobias Kind. Course: Search of MS/MS files with the NIST MS Search GUI

Welcome! Mass Spectrometry meets Cheminformatics WCMC Metabolomics Course 2014 Tobias Kind. Course: Search of MS/MS files with the NIST MS Search GUI Biology Informatics Chemistry Welcome! Mass Spectrometry meets Cheminformatics WCMC Metabolomics Course 2014 Tobias Kind Course: Search of MS/MS files with the NIST MS Search GUI http://fiehnlab.ucdavis.edu/staff/kind

More information

Shotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance.

Shotgun Proteomics MS/MS. Protein Mixture. proteolysis. Peptide Mixture. Time. Abundance. Abundance. m/z. Abundance. m/z 2. Abundance. Abundance Abundance Abundance Abundance Abundance Shotgun Proteomics Protein Mixture 1 2 3 MS/MS proteolysis m/z 2 3 Time µlc m/z MS 1 m/z Peptide Mixture m/z Block Diagram of a Mass Spectrometer Sample

More information

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification 22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and

More information

Proteomics/Peptidomics

Proteomics/Peptidomics Proteomics/Peptidomics System biology tools and preclinical models for translational research in endometriosis, ESHRE Campus workshop, 4-5 September 2009 E. Waelkens Proteomics: What? Proteins Proteomics

More information

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016 LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry

More information

Application Note # FTMS-46 solarix XR: Analysis of Complex Mixtures

Application Note # FTMS-46 solarix XR: Analysis of Complex Mixtures Application Note # FTMS-46 solarix XR: Analysis of Complex Mixtures Introduction Natural organic matter (NOM) as well as crude oil and crude oil fractions are very complex mixtures of organic compounds

More information

Agilent 7700x ICP-MS

Agilent 7700x ICP-MS Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler

More information

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010

MASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010 MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited

More information

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit

More information

Rapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer

Rapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer Robert Plumb, Michael D. Jones, and Marian Twohig Waters Corporation, Milford, MA, USA INTRODUCTION The detection and characterization of impurities and degradation products of an active pharmaceutical

More information

Edgar Naegele. Abstract

Edgar Naegele. Abstract Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in

More information

Supporting information

Supporting information Supporting information Figure legends Supplementary Table 1. Specific product ions obtained from fragmentation of lithium adducts in the positive ion mode comparing the different positional isomers of

More information

NIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05.

NIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05. NIH Public Access Author Manuscript Published in final edited form as: J Proteome Res. 2013 July 5; 12(7): 3071 3086. doi:10.1021/pr3011588. Evaluation and Optimization of Mass Spectrometric Settings during

More information

Metabolomics: quantifying the phenotype

Metabolomics: quantifying the phenotype Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems

More information

Amadeo R. Fernández-Alba

Amadeo R. Fernández-Alba % of compounds % of compounds % of compounds % of compounds Amadeo R. Fernández-Alba LC-Orbitrap QExactive Focus Instrumental LOQ 1% 9% 8% 7% 6% 5% 4% 3% 2% 1% %.1 mg/g.2 mg/g Tomato.5 mg/g ddms2 Target

More information

Biological Mass spectrometry in Protein Chemistry

Biological Mass spectrometry in Protein Chemistry Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of

More information

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses

More information

Increased Identification Coverage and Throughput for Complex Lipidomes

Increased Identification Coverage and Throughput for Complex Lipidomes Increased Identification Coverage and Throughput for Complex Lipidomes Reiko Kiyonami, David Peake, Yingying Huang, Thermo Fisher Scientific, San Jose, CA USA Application Note 607 Key Words Q Exactive

More information

N-terminal charge-driven de novo sequencing by using ASDF-incorporated Curved Field Reflectron

N-terminal charge-driven de novo sequencing by using ASDF-incorporated Curved Field Reflectron PO-CON1534E N-terminal charge-driven de novo sequencing by using ASDF-incorporated Curved Field Reflectron ASMS 2015 ThP 361 Yuzo Yamazaki 1 ; Keisuke Shima 1 ; Atsushi Kitanaka 2 ; Masahiro Miyashita

More information

SUPPORTING INFORMATION. Multimodal Mass Spectrometry Imaging of N-glycans and Proteins from the Same

SUPPORTING INFORMATION. Multimodal Mass Spectrometry Imaging of N-glycans and Proteins from the Same SUPPORTING INFORMATION Multimodal Mass Spectrometry Imaging of N-glycans and Proteins from the Same Tissue Section. Bram Heijs 1, Stephanie Holst 1, Inge H. Briaire-de Bruijn 2, Gabi W. van Pelt 3, Arnoud

More information

LECTURE 3. Ionization Techniques for Mass Spectrometry

LECTURE 3. Ionization Techniques for Mass Spectrometry LECTURE 3 Ionization Techniques for Mass Spectrometry Jack Henion, Ph.D. Emeritus Professor, Analytical Toxicology Cornell University Ithaca, NY 14850 Lecture 3, Page 1 Contents Electron ionization (EI)

More information

Choosing the metabolomics platform

Choosing the metabolomics platform Choosing the metabolomics platform Stephen Barnes, PhD Department of Pharmacology & Toxicology University of Alabama at Birmingham sbarnes@uab.edu Challenges Unlike DNA, RNA and proteins, the metabolome

More information

Extended Mass Range Triple Quadrupole for Routine Analysis of High Mass-to-charge Peptide Ions

Extended Mass Range Triple Quadrupole for Routine Analysis of High Mass-to-charge Peptide Ions Extended Mass Range Triple Quadrupole for Routine Analysis of High Mass-to-charge Peptide Ions Application Note Targeted Proteomics Authors Linfeng Wu, Christine A. Miller, Jordy Hsiao, Te-wei Chu, Behrooz

More information

MS/MS to Targeted Proteomics (MRM)

MS/MS to Targeted Proteomics (MRM) MS/MS to Targeted Proteomics (MRM) How it worked on the Human Lens Proteome Jayson Falkner PhD jay@singleorganism.com Genes Show Limited Value in Predicting Diseases With only a few exceptions, what the

More information

DART MSI of drugs of abuse in hair

DART MSI of drugs of abuse in hair DART MSI of drugs of abuse in hair Wilco Duvivier, Teris van Beek, Michel Nielen October 5, 2017 Forensic hair evidence Forensically interesting: Many compounds accumulate in hair Prolonged detectability

More information

Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System

Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System Marta Kozak Clinical Research Applications Group Thermo Fisher Scientific San Jose CA Clinical Research use only, Not for

More information

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19, MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents

More information

The New 6495 Triple Quadrupole LC/MS

The New 6495 Triple Quadrupole LC/MS The New 6495 Triple Quadrupole LC/MS Experience A New Level of Confidence Martin Haex Product Specialist MS and Automation 1 Overview of Topics The New 6495 QQQ LC/MS Technology Innovations Quantitation

More information

Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry

Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry PO-CON1347E Structural Elucidation of N-glycans Originating From Ovarian Cancer Cells Using High-Vacuum MALDI Mass Spectrometry ASMS 2013 TP-708 Matthew S. F. Choo 1,3 ; Roberto Castangia 2 ; Matthew E.

More information

Characterization of an Unknown Compound Using the LTQ Orbitrap

Characterization of an Unknown Compound Using the LTQ Orbitrap Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown

More information

An Introduction. 1 Introduction MALDI-TOF/TOF_version100623

An Introduction. 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF/TOF mass spectrometry An Introduction 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF(/TOF) mass spectrometry MALDI ion source Time-of of-flight (TOF) mass analyzer detector 2 Introduction

More information

Components of a Mass Spectrometer

Components of a Mass Spectrometer Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,

More information

Mass Spectrometry. Actual Instrumentation

Mass Spectrometry. Actual Instrumentation Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY

More information

On the Nature of the Chemical Noise in MALDI Mass Spectra

On the Nature of the Chemical Noise in MALDI Mass Spectra On the Nature of the Chemical Noise in MALDI Mass Spectra Andrew N. Krutchinsky and Brian T. Chait* Rockefeller University, New York, New York, USA The so-called chemical noise background imposes a major

More information

Parent and Neutral Loss Monitoring on a Quadrupole Ion Trap Mass Spectrometer: Screening of Acylcarnitines in Complex Mixtures

Parent and Neutral Loss Monitoring on a Quadrupole Ion Trap Mass Spectrometer: Screening of Acylcarnitines in Complex Mixtures Anal. Chem. 2002, 74, 5799-5806 Parent and Neutral Loss Monitoring on a Quadrupole Ion Trap Mass Spectrometer: Screening of Acylcarnitines in Complex Mixtures Joseph E. McClellan, Scott T. Quarmby, and

More information

Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS

Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS Analysis of Glycopeptides Using Porous Graphite Chromatography and LTQ Orbitrap XL ETD Hybrid MS Terry Zhang, Rosa Viner, Zhiqi Hao, Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA, USA Introduction

More information

ION MOTION AND AN OPTIMIZATION OF TANDEM MASS SPECTROMETRY

ION MOTION AND AN OPTIMIZATION OF TANDEM MASS SPECTROMETRY University of Kentucky UKnowledge University of Kentucky Master's Theses Graduate School 25 ION MOTION AND AN OPTIMIZATION OF TANDEM MASS SPECTROMETRY John Edward Spencer University of Kentucky, jespen2@uky.edu

More information

How to Use TOF and Q-TOF Mass Spectrometers

How to Use TOF and Q-TOF Mass Spectrometers How to Use TOF and Q-TOF Mass Spectrometers October 2011 What do TOF and Q-TOF offer? TOF Fast scanning of full spectrum High resolution full scan spectra Accurate mass measurements Q-TOF Fast scanning

More information

Towards High Resolution MS in Regulated Bioanalysis

Towards High Resolution MS in Regulated Bioanalysis Towards High Resolution MS in Regulated Bioanalysis Benno Ingelse MSD 3 rd EB focus meeting June 12 th 13 th Brussels, Belgium Contributors Gary Adamson Ken Anderson Kevin Bateman Cynthia Chavez-Eng Inhou

More information

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations

More information

MOMA: Mars Organic Molecule Analyzer Luann Becker Johns Hopkins Physics and Astronomy Harsh Environment Mass Spectrometry Workshop September 23rd

MOMA: Mars Organic Molecule Analyzer Luann Becker Johns Hopkins Physics and Astronomy Harsh Environment Mass Spectrometry Workshop September 23rd MOMA: Mars Organic Molecule Analyzer Luann Becker Johns Hopkins Physics and Astronomy Harsh Environment Mass Spectrometry Workshop September 23rd 2009 Strategy for Future Missions to Mars - Lessons learned

More information

Sue D Antonio Application Chemist Cedar Creek, TX

Sue D Antonio Application Chemist Cedar Creek, TX Sue D Antonio Application Chemist Cedar Creek, TX What is Hemp Oil? CBD hemp oil is a natural botanical extract of the common hemp plant. CBD hemp oil is derived from the seeds and stem of the Cannabis

More information

Characterization and Quantification of Covalent Modification of Proteins Using Mass Spectrometry

Characterization and Quantification of Covalent Modification of Proteins Using Mass Spectrometry City University of New York (CUNY) CUNY Academic Works Dissertations, Theses, and Capstone Projects Graduate Center 9-2018 Characterization and Quantification of Covalent Modification of Proteins Using

More information

Multiple Reaction Monitoring for Direct Quantitation of Intact Proteins using a Triple Quadrupole Mass Spectrometer

Multiple Reaction Monitoring for Direct Quantitation of Intact Proteins using a Triple Quadrupole Mass Spectrometer ELECTRONIC SUPPLEMENTARY INFORMATION Multiple Reaction Monitoring for Direct Quantitation of Intact Proteins using a Triple Quadrupole Mass Spectrometer Evelyn H. Wang 1, Peter C. Combe 2, and Kevin A.

More information

Protein Analysis using Electrospray Ionization Mass Spectroscopy *

Protein Analysis using Electrospray Ionization Mass Spectroscopy * OpenStax-CNX module: m38341 1 Protein Analysis using Electrospray Ionization Mass Spectroscopy * Wilhelm Kienast Andrew R. Barron This work is produced by OpenStax-CNX and licensed under the Creative Commons

More information

PosterREPRINT EVALUATION OF A RF-ONLY STACKED RING BASED COLLISION CELL WITH AXIAL FIELD FOR THE LC-MS-MS ANALYSIS OF THE METABOLITES OF RABEPRAZOLE

PosterREPRINT EVALUATION OF A RF-ONLY STACKED RING BASED COLLISION CELL WITH AXIAL FIELD FOR THE LC-MS-MS ANALYSIS OF THE METABOLITES OF RABEPRAZOLE OVERVIEW Comparison of the performance of a stacked ring based collision cell with axial field and a hexapole collision cell. Full scan LC-MS/MS-MS using precursor, product and neutral loss modes of operation

More information

Introduction to Proteomics 1.0

Introduction to Proteomics 1.0 Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using

More information

MSSimulator. Simulation of Mass Spectrometry Data. Chris Bielow, Stephan Aiche, Sandro Andreotti, Knut Reinert FU Berlin, Germany

MSSimulator. Simulation of Mass Spectrometry Data. Chris Bielow, Stephan Aiche, Sandro Andreotti, Knut Reinert FU Berlin, Germany Chris Bielow Algorithmic Bioinformatics, Institute for Computer Science MSSimulator Chris Bielow, Stephan Aiche, Sandro Andreotti, Knut Reinert FU Berlin, Germany Simulation of Mass Spectrometry Data Motivation

More information

5/11/2015 MASS SPECTROMETRY. Mass spectrometry (MS) - overview. Mass spectrometer. Ionization and mass analyzer. Analyzer: QUADRUPOLE

5/11/2015 MASS SPECTROMETRY. Mass spectrometry (MS) - overview. Mass spectrometer. Ionization and mass analyzer. Analyzer: QUADRUPOLE UNIVERSITY OF PÉCS MEDICAL SCHOOL www.medschool.pte.hu MASS SPECTROMETRY Mass spectrometry (MS) - overview SAMPLE: charged/ionized sample(fluid biological samples, proteins, peptides, oligosaccharides,

More information

Polymer Additive Analysis by EI and APCI

Polymer Additive Analysis by EI and APCI Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable

More information