Proximate composition, amino acid and fatty acid composition of fish maws. Department of Biology, Lingnan Normal University, Zhanjiang, , China

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1 SUPPLEMENTARY MATERIAL Proximate composition, amino acid and fatty acid composition of fish maws Jing Wen a, Ling Zeng b *, Youhou Xu c, Yulin Sun a, Ziming Chen b and Sigang Fan d a Department of Biology, Lingnan Normal University, Zhanjiang, , China b Department of Chemistry, Lingnan Normal University, Zhanjiang, , China c Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou, , China d Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture;South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou , China * Corresponding author. karine126@126.com Abstract Fish maws are commonly recommended and consumed in Asia over many centuries because it is believed to have some traditional medical properties. The present study highlights and provides new information on the proximate composition, amino acid and fatty acid composition of fish maws Cynoscion acoupa, Congresox talabonoides and Sciades proops. The results indicated that fish maws were excellent protein sources and low in fat content. The proteins in fish maws were rich in functional amino acids and the ratio of FAA: TAA (functional amino acids: total amino acids) in fish maws ranged from Among species, croaker C. acoupa contained most PUFA (polyunsaturated fatty acids), AA (arachidonic acid), DHA (docosahexaenoic acid) and EPA (eicosapntemacnioc acid), showing the lowest value of IA (index of atherogenicity) and IT (index of thrombogenicity), showing the highest value of HH (hypocholesterolemic / hypercholesterolemic ratio), which is the most desirable. Keywords: fish maw; proximate composition; amino acids; fatty acids 1

2 3. Experimental 3.1. Sample preparation The dried fish maw samples of C. acoupa, S. proops and C. talabonoides were purchased from the Yidelu dried seafood retail market in Guangzhou, China. For each species, there were 30 maw samples which were divided into three groups for three different analyses. The samples were ground using a grinder into fine powder. For each analysis, 100 g maw powder was used Proximate chemical composition Moisture, protein, fat and ash contents were determined according to AOAC (1990) Amino acid analysis Amino acid content of samples was measured according to Wen et al. (2010). Amino acids were expressed in mg/g of crude protein: amino acid content (% w/w) 1000 / crude protein content (% w/w) Amino acid score Essential amino acid scores were calculated according to reference amino acid requirements of adults (FAO/WHO/UNU, 2007) and child (FAO/WHO/UNU, 1985). The amino acid score was calculated using the following formula: amino acid score = amount of amino acid per test protein (mg g 1 ) / amount of amino acid per protein in reference pattern (mg g 1 ) Fatty acid analysis Fatty acid content of samples was measured according to Wen et al. (2010). Gas chromatography mass spectrometry (GC-MS) was performed on an Agilent Technologies 5973 instrument. Individual components were identified using mass spectral data and by comparing retention time data with those obtained for authentic and laboratory standards. Peak area was quantified and expressed as percentage of total fatty acids Indexes of fat quality The nutritional quality of the fats were determined from the data on the fatty acid composition, the following three indexes were calculated: (1) Index of atherogenicity (IA) (Ulbricht & Southgate, 1991): IA = [(C12:0 + (4 C14:0) + C16:0)]/ (Σ MUFA 2

3 + Σ n-6 PUFA + Σ n-3 PUFA). (2) Index of thrombogenicity (IT) (Ulbricht & Southgate, 1991): IT = (C14:0 + C16:0 + C18:0)/ [(0.5 Σ MUFA) + (0.5 Σ n-6 PUFA) + (3 Σ n-3 PUFA) + (Σ n-3 PUFA/ Σ n-6 PUFA)]. (3) The hypocholesterolaemic/ hypercholesterolaemic ratio (HH) (Santos-Silva, Bessa, & Santos-Silva, 2002): HH = (C18:1 cis-9 + C18:2 n-6 + C20:4 n-6 + C18:3 n-3 + C20:5 n-3 + C22:5 n-3 + C22:6 n-3)/ (C14:0 + C16:0) Statistical analysis All analyses were repeated in triplicate. Results were expressed as mean values ± standard deviation and analysis of variance were carried out using a statistical analysis system (SPSS Version 12). Differences between species were compared by the Tukey s test. Differences were considered to be significant when P <

4 Tables Table S1. Proximate composition (% w/w) of fish maws (mean values ± standard deviation). Species Moisture Protein Fat Ash Cynoscion acoupa ± 0.27a ± 0.24a 0.51 ± 0.01b 0.68 ± 0.01c Congresox talabonoides ± 0.28a ± 0.33b 1.08 ± 0.01a 1.40 ± 0.02b Sciades proops ± 0.38a ± 0.45b 0.56 ± 0.01b 1.83 ± 0.01a Values in the same column bearing different letters are significantly different (P < 0.05). 4

5 Table S2. Amino acid composition (mg/g crude protein) of fish maws (mean values ± standard deviation). Amino acid Cynoscion acoupa Congresox talabonoides Sciades proops Asp b 64.6 ± 0.99a 65.4 ± 1.09a 60.4 ± 0.88b Ser 31.0 ± 0.56a 29.2 ± 0.64a 33.2 ± 0.66a Glu b 119 ± 1.89a 107 ± 1.63c 113 ± 1.70b Gly b 216 ± 4.04a 215 ± 3.96a 213 ± 3.95a Ala 116 ± 1.84b 120 ± 1.89a 106 ± 1.77c Pro b 130 ± 2.14a 125 ± 2.04b 127 ± 2.08b Arg b 90.2 ± 1.67a 91.1 ± 1.73a 89.5 ± 1.66a Val a 24.8 ± 0.46b 30.9 ± 0.54a 31.6 ± 0.58a Met a, b 23.5 ± 0.42a 21.8 ± 0.39a 18.5 ± 0.33b Ile a 12.6 ± 0.28c 16.0 ± 0.36b 20.6 ± 0.42a Leu a, b 33.5 ± 0.65b 31.4 ± 0.58b 36.7 ± 0.76a Tyr a, b 9.00 ± 0.24b 10.4 ± 0.28b 18.5 ± 0.30a Phe a 25.5 ± 0.53b 23.7 ± 0.50b 28.0 ± 0.55a His a 9.10 ± 0.25c 13.4 ± 0.30b 17.3 ± 0.39a Lys a 45.9 ± 0.83a 43.2 ± 0.79a 37.2 ± 0.73b Thr a 37.2 ± 0.78a 32.3 ± 0.66b 38.0 ± 0.76a Trp a, b 7.12 ± 0.15a 8.09 ± 0.17a 7.51 ± 0.17a EAA / NEAA FAA / TAA Notes: Asp, aspartic acid; Ser, serine; Glu, glutamic acid; Gly, glycine; Ala, alanine; Pro, proline; Arg, arginine; Val, valine; Met, methionine; Ile, isoleucine; Leu, leucine; Tyr, tyrosine; Phe, phenylalanine; His, histidine; Lys, lysine; Thr, threonine; Trp, tryptophan; EAA / NEAA, essential amino acids / nonessential amino acids; FAA / TAA, functional amino acids / total amino acids. Values in the same row bearing different letters are significantly different (P < 0.05). a Essential amino acids. b Functional amino acids. 5

6 Table S3. Essential amino acids scores of fish maws. Amino acid Reference a Score (for adults) Reference b Score (for children) Cynoscion acoupa Congresox talabonoides Sciades proops Cynoscion acoupa Congresox talabonoides Sciades proops Val Met Ile Leu Phe + Tyr His Lys Thr Trp Abbreviations of amino acid are as in Table S2. a Reference amino acid requirements of adults (FAO/WHO/UNU, 2007). b Reference amino acid requirements of child (FAO/WHO/UNU, 1985). 6

7 Table S4. Fatty acid profiles (% of total fatty acids, mean values ± standard deviation) of fish maws. Fatty acid Cynoscion acoupa Congresox talabonoides Sciades proops C14: ± 0.05b 3.22 ± 0.05a 1.53 ± 0.03c C15: ± 0.02b 1.39 ± 0.02a 1.23 ± 0.03a C16: ± 0.58b ± 0.78a ± 0.82a C17: ± 0.02b 2.66 ± 0.04a 1.75 ± 0.02b C18: ± 0.61b ± 0.37c ± 0.56a C20: ± 0.01a 0.80 ± 0.01a 0.87 ± 0.01a C21:0 nd 0.20 ± 0.01 nd C22: ± 0.02b 0.46 ± 0.01c 0.86 ± 0.02a C23: ± 0.01b 0.19 ± 0.01b 0.33 ± 0.01a C24: ± 0.01a 0.26 ± 0.01b 0.59 ± 0.02a Σ SFA ± 0.28c ± 0.29b ± 0.27a C14:1 nd 0.18 nd C15: ± 0.03 nd nd C16:1 n ± 0.06a 3.69 ± 0.05a 3.13 ± 0.05b C17: ± 0.02b 0.82 ± 0.01a 0.45 ± 0.01b C18:1 n ± 0.36b ± 0.40a ± 0.33a C20:1 n ± 0.03a 0.82 ± 0.02c 1.99 ± 0.02b C22:1 n ± 0.01a 0.23 ± 0.01b nd C24:1 n ± 0.01a 0.82 ± 0.02b 0.69 ± 0.03b Σ MUFA ± 0.27a ± 0.31a ± 0.21a C18:2 n ± 0.06a 2.06 ± 0.03c 2.64 ± 0.03b C18:3 n-6 nd 0.96 ± 0.01 nd C20:2 n ± 0.01b 0.37 ± 0.01a nd C20:4 n-6 (AA) 5.75 ± 0.10a 4.39 ± 0.06b 3.93 ± 0.06c Σ n ± 0.05a 7.78 ± 0.06b 6.57 ± 0.04c C18:3 n ± 0.01a 0.28 ± 0.01b nd C20:3 n ± 0.01b 0.55 ± 0.02a nd C20:5 n-3 (EPA) 2.26 ± 0.02a 1.23 ± 0.02b 0.42 ± 0.01c C22:6 n-3 (DHA) 7.20 ± 0.15a 5.02 ± 0.09b 1.50 ± 0.02c EPA + DHA 9.46 ± 0.08a 6.25 ± 0.05b 1.92 ± 0.03c Σ n ± 0.07a 7.08 ± 0.06b 1.92 ± 0.03c Σ PUFA ± 0.09a ± 0.06b 8.49 ± 0.06c Notes: nd, not detected; SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; n-6, n-6 PUFA; n-3, n-3 PUFA. Values in the same row bearing different letters are significantly different (P < 0.05). 7

8 Table S5. Indexes of fat quality in fish maws. Species Σ PUFA / Σ SFA Σ n-3 / Σ n-6 IA IT HH Cynoscion acoupa Congresox talabonoides Sciades proops Abbreviations of fatty acid are as in Table S4. Notes: IA, index of atherogenicity; IT, index of thrombogenicity; HH, hypocholesterolemic / hypercholesterolemic ratio. 8