The One Minute Chromatographer
|
|
- Jason Horton
- 6 years ago
- Views:
Transcription
1 The One Minute Chromatographer Agilent Technologies, Inc Page 1
2 Sub 1 Minute Separations with RRHD Columns Agilent ZORBAX RRHD SB-C18, 2.1 x 100mm, 1.8 µm H2O (0.05% Formic acid) / 2-98 % Acetonitrile T = 80 C V_i = 0.5 µl WL = 210 nm DR = 80 Hz F = 2 ml/min P = 1085 bar
3 Plate Height H Smaller Particles Reduce In Column Dispersion Increased Efficiency H = A + B/u + C u Large Particle Small Particle Sum Curve: van-deemter H min Longitudinal diffusion Resistance to Mass Transfer Eddy Diffusion u opt Linear Velocity u Page 3
4 Two Advances For Better Mass Transfer SOLID CORE Sub 2um Particle Superficially Porous Particle
5 Poroshell 120 Columns for HPLC and UHPLC: Poroshell 120 columns have: 80-90% efficiency of sub 2um At ~40-50% lower pressure 2X efficiency of 3.5um (totally porous) A 2.7um particle size A 2um frit to reduce clogging A 600 bar pressure limit The particle has a solid core (1.7um) and porous outer layer with a 0.5um diffusion path 0.5um 1.7um 0.5um Confidentiality Label 5 July 17, 2013
6 Poroshell 120 Several Different Phases EC-C18 EC-C8 EC-CN Stablebond C18 Stablebond C 8 Stablebond Aq Bonus RP HILIC Phenyl Hexyl Page 6
7 HETP (cm) Van Deemter Redux van Deemter Eclipse Plus C18, 3.5 µm Eclipse Plus C18, 1.8 µm Poroshell 120 C18, 2.6um Poroshell 120 C18, 2.6 µm Poroshell 120 C18, 2.7 µm Flow rate (ml/min) 7
8 High Peak Capacity Maximizes Resolution with Complex Samples 1.8u Particles
9 High Peak Capacity Maximizes Resolution with Complex Samples Poroshell 120
10 Smaller Particles Improve Detection and Integration 4 Impurities 2 Not Baseline Separated! 7 Impurities 6 Not Baseline Separated! 7 Impurities All 7 Baseline Separated! 4.6 x 150, 5μm 93 bar 4.6 x 150, 3.5μm 165 bar Customer Sample, Impurity Method 4.6 x 150, 1.8μm 490 bar Page 10
11 What is Sensitivity? Limit of detection (LOD): The lowest concentration, or smallest mass flow, which can be distinguished from the noise by a certain predefined probability (Signal/Noise). Signal Noise Limit of detection (LOD): h Signal = 3(2) x h Noise Page 11
12 Analysis of 15 Analgesic Compounds Same Method for all Three Columns ZORBAX Eclipse Plus C18, 3 x 100 mm, 3.5 µm Ibuprofen: PW 1/2 =0.014 S/N=182 n c =43 ZORBAX RRHD Eclipse Plus C18, 3 x 100 mm, 1.8 µm Ibuprofen: PW 1/2 =0.012 S/N=353 n c =54 Poroshell 120 EC-C18, 3 x 100 mm, 2.7 µm Ibuprofen: PW 1/2 =0.012 S/N=256 n c =56 2 min June, 2011
13 Comparison of 4.6 x 250 mm 5 um to Poroshell 120 EC-C x 100 mm, 2.7um mau Time %B Column: Eclipse Plus C x 250mm, 5um Flow Rate: 1 ml/min 110 bar Mobile Phase: A: 0.1% formic acid in Water B: 0.1% formic acid in ACN Sulfadiazine, Sulfathiazole Sulfapyridine Sulfamerazine, Sulfamethazine, Sulfamethazole, Sulfamethoxypyridazine, Sulfachloropyridazine Sulfamethoxazole, Sulfadimethoxine 0 mau Time %B Column: 4.6 x 100mm Poroshell 120 EC-C18, 2.7um Flow Rate: 1 ml/min 325 bar min min
14 Expand High Speed Chromatograms for True Comparison to Slower Separation See conditions previous slide mau bar 20 mau min bar min
15 Rapid Resolution LC Ultra Fast, Resolution Maintained, Sensitivity Improved Optimized Gradient 5 Run Time 1.8 min Equilibration 1 min Time Total Analysis 2.8 min Time 480 Samples/day Column: Rapid Resolution Eclipse XDB-C8, 4.6 x 50 mm, 3.5 mm Mobile Phase: A: 55% 25 mm Na 2 HPO 4, ph 3 B: 45% MeOH Gradient: 42 90% B in 2 min Flow Rate: 3 ml/min Temperature: 35 C Sample: Cardiac Drugs 1. Diltiazem 2. Dipyridamole 3. Nifedipine 4. Lidoflazine 5. Flunarizine Time (min) Page 15
16 US. Pharmacopeia 621 Column length: ± 70 % Column internal diameter: ± 25 % Column material particle size: Reduction of up to 50 %, no increase Flow rate: ± 50 % Injection volume: Changes are allowed as long as system suitability testing (SST) criteria are met. Column temperature: ± 10 % ph of mobile phase: ± 0.2 UV wavelength: no change outside manufacturer specifications Concentration of salts in buffer: ± 10 % Composition of mobile phase (adjustment of the minor component is allowed ±30 % or ± 10 % absolute whichever is smaller) Page 16
17 USP and FDA Method Adjustment Criteria Method Adjustment Criteria for Column Dimensions Parameter Maximum Specifications Comments/Examples Column Length ± 70% 250mm 75mm 150mm 50mm ±25% (FDA ORA-LAB 5.4.5*) 4.6 mm 3.0 mm (-35%) Column Internal Diameter USP ± 25% Column ID can be adjusted provided linear velocity is constant** Flow Rate ±50% Injection Volume Particle Size Reduce as much as needed must still meet detection limits and precision Reduce by up to 50% (can not be increased) 4.6 mm 2.1 mm (-54%) 3.0 mm 2.1 mm (-30%) If you change to a smaller/ shorter column make the appropriate change in injection volume You can change column length and particle size to keep Rs same 5um 3.5um (-30%) 5um 2.7um (-46%) *Changed on 2/24/09 - For the current and official copy, go to ** USP 30 Second Supplement Revisions, PF34(5), in process expected to be final Dec 2009 See Stimuli article in Pharmacopeial Forum 2009; 35(6) Page 17
18 USP Method-Naproxen Page 18
19 Naproxen 100 mm column Page 19
20 NAPROXEN 50 mm Column Page 20
21 Agilent 1200 Rapid Resolution System Speed Conventional LC UFLC RRLC F= 1.20ml/min T = 40 C Analysis Time = 11min Solvent Cons. = 13.2ml High Resolution: 4.6mm x 150mm 5.0µm min F = 4.80ml/min T = 40 C Analysis Time = 1.05min Solvent Cons. = 5.1ml High Speed: 4.6mm x 50mm 5.0µm min F= 1.00ml/min T = 40 C Analysis Time = 1.1min Solvent Cons. = 1.1ml High Speed & Resolution: 2.1mm x 50mm 1.8µm min F= 2.40ml/min T = 95 C Analysis Time: 0.4min Solvent Cons. = 1.0ml PW HH = 197msec > 20x faster! Max Speed at T = 95 o C 2.1mm x 50mm 1.8um min Page 21
22 100% B t g = 5 Changing Gradient Time to Affect Retention (k*) and Resolution 0% B 100% B k* = t g F S D%B V m t g = 10 1/k* = gradient steepness = b 0% B 100% B DF = change in volume fraction of B solvent 0% B t g = 20 S = constant F = flow rate (ml/min.) t g = gradient time (min.) V m = column void volume (ml) 100% B t g = 40 0% B Time (min) S 4 5 for small molecules 10 < S < 1000 for peptides and proteins P1.PPT Group/Presentation Title Agilent Restricted Month ##, 200X
23 Very Fast LC on Conventional 1100 HPLC G1379 Degasser G1311 Quaternary pump G1313A ALS autosampler G1316A column compartment The Instrument G1314A VWD (standard cell G , 10mm, 14uL) Acetophenone Diethyl phthalate Benzophenone Butyrophenone Valerophenone Hexanophenone Heptanophenone Octanophenone The Sample Page 23
24 Conventional Column x 150mm, 5µm, SB-C18 mau VWD1 A, Wavelength=246 nm (D:\SAMPLE TEST\RRHT-1100\070809SBC D) Flow Rate 1.0 ml/min Injection Volume 15uL Temperature 30 C Wavelength 246nm Sample rate 2.5 Hz 150 Time (min) % Acetonitrile Initial Pressure: 69 bar Final Pressure: 38 bar min Page 24
25 Shorten Column and Gradient Time by Same Factor 1/3 Column Length- 1/3 Gradient Time RRHT Column 4.6 x 50mm, 1.8µm, SB-C18 mau VWD1 A, Wavelength=246 nm (D:\SAMPLE TEST\RRHT-1100\HDS \070809SBC D) Flow Rate 1.0 ml/min Injection Volume 5uL Temperature 30 C Wavelength 246nm Sample rate Hz Time (min) % Acetonitrile Initial Pressure: 132 bar Final Pressure: 74 bar min Page 25
26 Increase Column Flow-Reduce Gradient Time Double Flow (2mL/min) ½ Gradient Time RRHT 4.6 x 50mm, 1.8µm, SB-C18 mau VWD1 A, Wavelength=246 nm (D:\SAMPLE TEST\RRHT-1100\HDS \070810SBC D) Flow Rate 2.0 ml/min Injection Volume 5uL Temperature 30 C Wavelength 246nm Sample rate Hz Time (min) % Acetonitrile min Initial Pressure: 266 bar Final Pressure: 146 bar Page 26
27 What Length Column Yields Highest Peak Capacity? Flow rate vs. Gradient time vs. Peak capacity For small molecules(mw < ~1000) 150mm 100mm 50mm Different Column Lengths Broken lines are isobar (800 bar)
28 Shorter Columns with Fast Gradients Yield Higher Peak Capacity Shorter Gradient (5 min) 150mm 100mm 50mm Peak Capacity: 258 for 50 mm 240 for 100 mm 221 for 150 mm
29 Longer Columns with Long Gradient Times Yield Greater Peak Capacity Long Gradient (40 min) Peak Capacity: 422 for 50 mm 150mm 100mm 50mm 510 for 100 mm 525 for 150 mm
30 Constant Particle Size, Grad Time More Resolution mau Rs: 0 RRHD SB-C x 50 mm, 1.8um Pmax=366 bar n c = mau min Rs: 1.37 RRHD SB-C x 100 mm, 1.8um Pmax=595 bar n c = mau Rs: 2.40 min RRHD SB-C x 150 mm, 1.8um Pmax=768 bar n c = min Page 30 Group/Presentation Title Agilent Restricted Month ##, 200X
31 Poroshell 120, 150mm Columns HPLC or UHPLC Conditions: Column: Poroshell 120 EC-C18, 4.6 x 150mm, 2.7um Mobile Phase: Solvent A: Water with 0.1% Formic Acid Solvent B: Acetonitrile 1200 SL controlled temperature at 25 C 2 ul flow cell mau ml/min P = 538 bar 1. Hydroquinone 2. Resourcinol 3. Catechol 4. Phenol 5. 4-Nitrophenol 6. p-cresol 7. o-cresol 8. 2-Nitrophenol 9. 3,4 di methyl phenol 10. 2,3 di methyl phenol 11. 2,5 di methyl phenol napthol min mau ml/min P = 285 bar Gradient: 1mL/min Time %B 6.0 5% 51 60% Gradient: 2mL/min Time %B 3.0 5% % min
32 UHPLC/TOF (1290/6230) Can Identify More Compounds in Less Time 224 pesticides at 50 pg each 217 ionized & detected in positive mode (97%, Find by Formula) 2.1 x 50 mm x 1.8 micron Eclipse Plus C bar 1.5 ml/min 1290 Infinity Time Composition 1.5 min % ACN % ACN TOF fast acquisition rates (20Hz) ensure maximum throughpu Page 32
33 Ultrafast LC/MS Analysis for 15 Analyte Subset RRHD Eclipse Plus C18 2.1x 50 mm, 1.8 um 750 bar 1 minute Peak Width 0.7 sec Time Composition % ACN % ACN Ultimate speed on a short column with ballistic gradient 1290 Infinity Applications
34 Method Translator Makes Changes Easy Let Agilent Method Translator Do the Math Basic Mode for Easy Transfer of Conventional Method to RRLC Injection Volume Conversion Detector Settings recommendation Gradient and Isocratic Method Conversion (autodetected) Page 34
35 Agilent Method Translator Advanced Mode More Detaled Information, But Still Easy to Use Detailed Input Detailed Output Original Method New Method Page 35
36 Page 36 Systems/Liquid-Chromatography/pages/1200infinity_cost_calculator.aspx
37 RULE OF THUMB Set of Approximations based on chromatographic behavior and mathematical relationships Will deliver nearly the desired goal Probably need to be tweaked to deliver best results
38 What Is Your Current Column Efficiency and How Fast Do You Want To Run? Column Length (mm) Resolving Power N(5 µm) Resolving Power N(3.5 µm) Resolving Power N(1.8 µm) Typical Pressure Bar (1.8 µm) Analysis Time* ,500 21,000 32, ,500 14,000 24, ,500 17, ,200 7,000 12, N.A. 4,200 6, N.A. 2,100 2, Analysis Time Peak Volume Solvent Usage -33% -50% -67% -80% -90% * Reduction in analysis time compared to 150 mm column pressure determined with 60:40 MeOH/water, 1ml/min, 4.6mm ID Page 38
39 First, Reduce Column Length 5um 1.8um Reduce column length by factor of 3 Quite often original method will have more resolution than is actually needed and a reduction by 5 may be possible 3.5 um 1.8um Reduce column length by factor of 2 Quite often original method will have more resolution than is actually needed and a reduction by 3 is possible Page 39
40 Flow Modification - Different Diameter Columns Flow col. 1 Diam. Diam. column2 column1 2 Flow col. 2 i.e mm 1.0ml/min 0. 21ml/min 4.6mm Page 40
41 Decreased Column Volume May Require Conversion for Injection Volume Inj.Vol. col. 1 Volume Volume column2 column1 Inj.Vol. col. 2 Zorbax column volume = 3.14 x r 2 x L x 0.6 (r and L in cm) i.e. 20μl 0.4ml column2 col ml column1 μl col. 2 Page 41
42 Reduce injection volume Reduction to allow for diameter change 4.6 mm 3 mm = 0.4 x Original 4.6 mm 2.1 mm = 0.2 x Original x Reduction to allow for length change 150 mm 50 mm = 0.33 x Original 150 mm 1100 mm = 0.67 x Original 100 mm 50 mm = 0.5 x Original e.g. Original 4.6mm x 150mm transferred to 2.1mm x 100mm = 0.2 x 0.67 =0.13 x original injection volume Page 42
43 Simple Isocratic Method Transfer for Speed Step 1 Reduce Column Length and Particle Size Maintain Flow Rate Step 2 Faster Increase Flow Rate Pressure too High? Increase Temperature to Lower Pressure Increase Flow Rate Step 3 Even Faster (Need Instrument Optimization) Decrease Column Diameter (2.1mm), Reduce Flow Rate Proportionately Reduce Injection Volume Based on Ratio of Column Volumes Increase Flow Rate Page 43
44 Simplified Gradient Method Transfer Step 1 Reduce Column Length and Particle Size Adjust Gradient Time by same Factor Maintain Flow Rate Step 2 Faster Increase Flow and Reduce Gradient Time Stop When Reach Flow Limits of Instrument Step 3 Even Faster Decrease Diameter of Column (2.1mm) Match Flow to New Column Diameter Reduce Injection Volume Repeat Step 2 Until Reach % Instrument Pressure Limit Page 44
45 Let s Look at Method Development of These Compounds propranolol pindolol dipyridamole β-blocker Anti-arrhythmic Vasodilator Ca+ channel blocker disopyramide diltiazem Group/Presentation Title Agilent Restricted Month ##, 200X
46 Start at Low ph, Adjust Organic Eclipse Plus C18 Cardiac Drugs with Acetonitrile mau mau mau Column: ZORBAX RRHT Eclipse Plus C18, 4.6 x 50 mm, 1.8 mm Mobile Phase: A: 25 mm NaH 2 PO 4, ph 3.0 B: ACN Flow Rate: 2.0 ml/min Temperature: 30 C Detection: UV 240 nm Sample: Cardiac Drugs 1.Pindolol 2. Diisopyridamide 3.Propranolol 4.Dipyridamole 5. Diltiazem 40% ACN % ACN RRHT Eclipse Plus C18 allows for very fast optimization of % organic in mobile phase. 20% ACN Good resolution Fast analysis No time wasted To get this k on a 25cm column at 2 ml/min would require 1.5 hours run time!! k= 44!! min Group/Presentation Title Agilent Restricted Month ##, 200X
47 Start at Low ph on Eclipse Plus C18 Cardiac Drugs with Methanol, Changing Organic Modifier mau mau Column: ZORBAX RRHT Eclipse Plus C18, 4.6 x 50 mm, 1.8 mm Mobile Phase: A: 25 mm NaH 2 PO 4, ph 3.0 B: MeOH Flow Rate: 2.0 ml/min Temperature: 30 C Detection: UV 240 nm Sample: Cardiac Drugs 1.Pindolol 2. Diisopyridamide 3.Propranolol 4.Diltiazem 5. Dipyridamole 70% MeOH % MeOH Adjusting MeOH influences retention like adjusting ACN Solvents can be compared quickly with RRHT columns MeOH changes selectivity and gives longer analysis time for these analytes mau % MeOH k= 49!! min Group/Presentation Title Agilent Restricted Month ##, 200X
48 Viscosity When to Stop!? Viscosity When flow limit of pump is reached Consider using a smaller i.d. column When approaching about 90% pressure limit of instrument 540 bar for 1200SL pump 360 bar for 1100/1200 pump Remember to allow for increase in pressure at non-ideal mix When resolution is no longer satisfactory ACN/water 40C Methanol/water 40C %ACN %Methanol
49 Agilent 1.8u and Poroshell 120 Columns Make High Resolution, Fast LC Possible on All Instruments Reduce Analysis Time Speed Up Method Development Improves Methods on HPLC Provides Best Benefits on UHPLC Coupled with UHPLC Instruments Allow Higher Flow Rates for Higher Resolution Without Long Run Times Page 49
Maximizing Efficiency Using Agilent InfinityLab Poroshell 120 Columns
Maximizing Efficiency Using Agilent InfinityLab Poroshell 12 Columns 1, Plates in Less Than Minutes Using Coupled Column Technology Application Note Food, Environmental, Chemical, Pharmaceutical Authors
More informationNew HPLC Column Options for UHPLC
New HPLC Column Options for UHPLC Rapid Resolution High Definition Columns and More Chor Koon Tan, PhD Business Development Manager Columns & Supplies Division Asia-Pacific Agilent Technologies Page 1
More informationEffective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate
Effective use of Pharmacopeia guidelines to reduce cost of chromatographic analysis for Fluticasone propionate Application Note Pharmaceutical QA/QC Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More informationPerformance Characteristics of the Agilent 1290 Infinity II Multicolumn Thermostat
Performance Characteristics of the Agilent 9 Infinity II Multicolumn Thermostat Technical Overview Author Sonja Schneider Agilent Technologies, Inc. Waldbronn, Germany Abstract This Technical Overview
More informationRapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit
Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent
More informationMaximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system
Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author
More informationIncreasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 1290 Infinity LC System
Increasing resolution using longer columns while maintaining analysis time Advantages of the wide power range of the Agilent 129 Infinity LC System Application Note Pharmaceutical and Chemical Analysis
More informationAm I getting the very best value from my UHPLC analyses?
Am I getting the very best value from my UHPLC analyses? Stephen Luke LC Columns Product Manager 1 Primary reasons for UHPLC use Very fast Very high resolution Column 2.1 x 50 mm Column 2.1 x 150 mm Run
More informationAPPLICATIONS TN Overview of Kinetex 2.6 µm Core-Shell Technology
Determination of Impurities and Related Substances for Acetylsalicylic Acid (Ph. Eur. Monograph 9): Increased Sensitivity and Resolution, Faster Analysis, and Reduced Solvent Usage Using Kinetex 2.6 µm
More informationEvaluation of UHPLC System Performance Under High Throughput Conditions. William Hedgepeth, Masatoshi Takahashi, Shimadzu Scientific Instruments
Evaluation of UHPLC System Performance Under High Throughput Conditions William Hedgepeth, Masatoshi Takahashi, Shimadzu Scientific Instruments Introduction The use of UHPLC technology has continued to
More informationThe LC Column Playbook
The LC Column Playbook Mark Powell Applications Engineer Technical Support 1 Overview How do we achieve our goal: Resolution? Efficiency Particle size Column length Selectivity Bonded phase Mobile phase
More informationAnalytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 120 PFP
Analytical Method Development for USP Related Compounds in Paclitaxel Using an Agilent Poroshell 1 PFP Application Note Clinical Research Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd Abstract
More informationConverting a CHP Method for Insulin to Agilent Poroshell 120 Columns
Converting a CHP Method for Insulin to Agilent Poroshell Columns Application Note Biopharm Author Rongjie Fu Agilent Technologies (Shanghai) Co., Ltd. 4 Ying Lun Road Shanghai 3 China Abstract A regulatory
More information11:00 a.m. EST Telephone Number: Chair Person: Tim Spaeder
Stephen W. Coates LC Columns Marketing Specialist Consumables and Accessories Business Unit May 17, 2 High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry, and Rapid Method Development
More informationHPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC
HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 129 Infinity II LC Application Note Small Molecule Pharmaceuticals Authors Gerd Vanhoenacker, Mieke Steenbeke, Koen Sandra,
More informationPhenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases
Phenyl-Hexyl UHPLC Columns lternate, complementary selectivity to A C8 and C8 bonded phases articularly recommended for P compounds containing aromatic groups xcellent bonded phase stability for E durable,
More informationComparison of a UPLC Method across Multiple UHPLC Systems
Comparison of a UPLC Method across Multiple UHPLC Systems Tanya Jenkins Waters Corporation, Milford, MA, U.S. INTRODUCTION In 2004, Waters introduced the ACQUITY UPLC System. Since this launch, many liquid
More informationApplication Note. Small Molecule Pharmaceuticals. 2-Ethyl-2-phenylmalonamide Ethosuximide Primidone Carbamazepine. Carbamazepine-
Analysis of Pharmaceutical Substances Using HPLC and UHPLC Methods Backward Compatibility for Validated Analytical Procedures and Methods Comparing the Infi nity LC and the Infi nity II LC Application
More informationApplication Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.
Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2
More informationSelectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants
Selectivity Comparison of Agilent Poroshell 1 Phases in the Separation of Butter Antioxidants Application Note Food Testing & Agriculture Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd. Abstract
More informationHigh-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System
High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System Application Note Food, Hydrocarbon Processing Authors Michael Woodman Agilent Technologies,
More informationMass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System
Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent
More informationTransferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC
Transferring a Method for Analysis of DNPH-Derivatized Aldehydes and Ketones from HPLC to UHPLC Application Note Environmental Author Melanie Metzlaff Agilent Technologies, Inc. Waldbronn, Germany Abstract
More informationApplication Note. Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling. Abstract.
Agilent Application Solution Analysis of fat-soluble vitamins from food matrix for nutrition labeling Application Note Food 1 Agilent 12 Infinity 1 Author Siji Joseph Agilent Technologies, Inc. Bangalore,
More information2D-LC as an Automated Desalting Tool for MSD Analysis
2D-LC as an Automated Desalting Tool for MSD Analysis Direct Mass Selective Detection of a Pharmaceutical Peptide from an MS-Incompatible USP Method Application Note Biologics and Biosimilars Author Sonja
More informationUSP purity analysis of pravastatin sodium using the Agilent 1120 Compact LC
USP purity analysis of pravastatin sodium using the Agilent 1120 Compact LC Application Note Manufacturing QA/QC Authors Syed S. Lateef, Siji Joseph Agilent Technologies Bangalore, India 500 400 Pravastatin
More informationOpiates, Opioids and Benzodiazepines, Amphetamines & Illicit Drug Forensic Analysis by LC/MS
Opiates, Opioids and Benzodiazepines, Amphetamines & Illicit Drug Forensic Analysis by LC/MS Julie Cichelli, PhD Agilent Technologies Application Engineer April 29, 2014 Agenda A method for the rapid analysis
More informationThe high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns.
The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. Fast High peak capacity Low back pressure PROTEIN PEPTIDE UHPLC COLUMNS Put these new, high-performance
More informationFor Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns.
For Far Greater UHPLC Column Performance... You Must Have Kinetex Core-Shell UHPLC Columns www.phenomenex.com/kinetex The UHPLC Core-Shell Advantage Kinetex Core-Shell Technology produces increased efficiencies
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationAeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE
Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance
More informationUltra High Performance Liquid Chromatograph. Nexera X2 C196-E079
Ultra High Performance Liquid Chromatograph Nexera X2 C196-E79 Maximizing the Potential of UHPLC/HPLC Analyses Intelligence Intelligent data solutions, including a new i -PDeA peak calculation method for
More informationUltrafast analysis of synthetic antioxidants in vegetable oils using the Agilent 1290 Infinity LC system
Ultrafast analysis of synthetic antioxidants in vegetable oils using the Agilent 19 Infinity LC system Application Note Food Authors Standard solution Gerd Vanhoenacker, Frank David, Pat Sandra Research
More informationHyper-fast & Super-rugged
Hyper-fast & Super-rugged UHPLC & HPLC columns FALL 2013 PRODUCT CATALOG Hyper-fast & Super-rugged UHPLC & HPLC columns he introduction of HALO columns, developed using innovative Fused-Core particle
More informationRestek Ultra II HPLC Columns
Restek Ultra II HPLC Columns THE Choice for All U(HPLC) Systems November 2009 1 www.chromtech.net.au, sales@chromtech.net.au, Tel: 03 9762 2034, Fax: 03 9761 1169 Topics for Today Introducing Restek Ultra
More informationFat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C18 Bonded Column
Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C8 Bonded Column Application Note Food Authors Rongjie FU Agilent Technologies (Shanghai) Co. Ltd. Ying Lun Road Waigaoqiao Free
More informationTransfer and Speed-up of Methods to Fused-Core Particle Columns EPA Method 1694
Transfer and Speed-up of Methods to Fused-Core Particle Columns EPA Method 1694 Ed Mauney, Hillel K. Brandes, William H. Campbell, Wayne K. Way, Richard A. Henry, David S. Bell, and Carmen T. Santasania
More informationCore-Shell / Solid core Particles: A Practical Substitute to Sub-2 Micron Particles: An overview
Core-Shell / Solid core Particles: A Practical Substitute to Sub-2 Micron Particles: An overview ABSTRACT: S. M. Ramdharane 1, K. B. Vyas 2, K. S. Nimavat 3 * 1 Pacific Academy of Higher Education & Research
More informationAPPLICATIONS Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns
Improving Intact Biogeneric Protein Separations with Aeris WIDEPORE Core-Shell Columns Michael McGinley, Deborah Jarrett, and Jeff Layne Phenomenex, Inc., 411 Madrid Avenue, Torrance, CA 91 USA Aeris WIDEPORE
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic
More informationThe Raptor HILIC-Si Column
The Raptor HILIC-Si Column With Raptor LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or core-shell particles) with the resolution
More informationF. Al-Rimawi* Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, East Jerusalem. Abstract
JJC Jordan Journal of Chemistry Vol. 4 No.4, 2009, pp. 357-365 Development and Validation of Analytical Method for Fluconazole and Fluconazole Related Compounds (A, B, and C) in Capsule Formulations by
More informationCannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system
Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D
More informationACE Excel UHPLC Columns
Excel UHPLC Columns info@ace-hplc.com Advanced Chromatography Technologies Ltd www.ace-hplc.com Australia & New Zealand contact: infor@winlab.com.au or call + 0 Outline Introduction Key Product Points
More informationSue D Antonio Application Chemist Cedar Creek, TX
Sue D Antonio Application Chemist Cedar Creek, TX What is Hemp Oil? CBD hemp oil is a natural botanical extract of the common hemp plant. CBD hemp oil is derived from the seeds and stem of the Cannabis
More informationDevelopment and Validation of a Polysorbate 20 Assay in a Therapeutic Antibody Formulation by RP-HPLC and Charged Aerosol Detector (CAD)
LIFE SCIENCES AR ENOUGH? HOW DO YOU KNOW RAW MATERIALS ARE PURE? HOW DO YOU EVALUATE PRODUCT PACKAGING? HOW DO YOU DO YOU KNOW WHAT ANALYTICAL TECHNIQUE TO USE? HOW DO YOU SIMULTANEOUSLY TEST FOR TWO BYPRODUCTS?
More informationHigh-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development
Robert Ricker Bio-Applications Scientist CABU April 5, 2 Rapid Resolution HPLC High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development Slide 4 Ultra-Fast
More informationFused-Core Particles:
Fused-Core Particles: Varying Shell Thickness and Pore Size Stephanie A. Schuster; Joseph J. Kirkland; Brian M. Wagner; Barry E. Boyes; William L. Johnson; Timothy J. Langlois; Joseph J. DeStefano Advanced
More informationApplication Note. Authors. Abstract. Food
Determination of Hormones in Shrimp by Agilent 129 Infinity LC with Agilent Poroshell 12 LC Column and Agilent Bond Elut QuEChERS for Sample Preparation Application Note Food Authors Rongjie Fu and Andy
More informationACQUITY UPLC WITH PDA DETECTION: DETERMINING THE SENSITIVITY LIMITS OF OXYBUTYNIN AND RELATED COMPOUNDS
ACQUITY UPLC WITH PDA DETECTION: DETERMINING THE SENSITIVITY LIMITS OF OXYBUTYNIN AND RELATED COMPOUNDS Tanya Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Some of the most challenging methods
More informationExtended Application Note
Extended Application Note Comparison of 4 μm vs. 2.o Columns in Analysis of Drospirenone/Ethinyl Estradiol Tablet APP A-339 www.mtc-usa.com 1-732-578-1777 INTRODUCTION The formulation of drospirenone and
More informationChiral Multicolumn Method Development on the Agilent 1260 Infinity II SFC System
Chiral Multicolumn Method Development on the Agilent 126 Infinity II SFC System Application Note Small Molecule Pharmaceuticals Author Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany Abstract
More informationUltra High Definition Optimizing all Analytical Dimensions
Ultra High Definition Optimizing all Analytical Dimensions Sensitivity Dynamic Range Signal Response Linearity Separation Speed Peak Capacity Chromatogram Mass Spectrum Mass Accuracy Resolving Power Acquisition
More informationChiral Screening for SFC and UHPLC with the Agilent 1260 Infinity II SFC/UHPLC Hybrid System
Application Note Small Molecule Pharmaceutical Chiral Screening for SFC and UHPLC with the Agilent 16 Infinity II SFC/UHPLC Hybrid System Author Edgar Naegele, Agilent Technologies, Inc. Waldbronn, Germany
More informationApplication Note. Abstract. Authors. Pharmaceutical
Analysis of xycodone and Its Metabolites-oroxycodone, xymorphone, and oroxymorphone in Plasma by LC/MS with an Agilent ZRBAX StableBond SB-C18 LC Column Application ote Pharmaceutical Authors Linda L.
More informationAPPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology.
Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology Jeff Layne, Phenomenex, Inc. Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 951 USA With virtually
More informationExtended Temperature Range Impact on HPLC Column Stability and Performance
Extended Temperature Range Impact on HPLC Column Stability and Performance Stephanie J. Marin Dale Felix, Jody Clark Selerity Technologies, Inc. Salt Lake City, UT www.selerity.com The Selerity Series
More informationAnalysis of bisphenol A leaching from baby feeding bottles
Agilent Application Solution Analysis of bisphenol A leaching from baby feeding bottles Application Note Consumer Products Author Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India Abstract
More informationImpurity Profiling of Carbamazepine by HPLC/UV
Application Note: 52049 Impurity Profiling of Carbamazepine by HPLC/UV Terry Zhang, Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela Hypersil GOLD Carbamazepine Drug Analysis
More informationQualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution
Qualitative and quantitative determination of phenolic antioxidant compounds in red wine and fruit juice with the Agilent 1290 Infinity 2D-LC Solution Application Note Food Testing Author Edgar Naegele
More informationACE C18-AR Use the Power!
combines the retention and separation characteristics of a C18 phase with the extra selectivity of an integral phenyl functional group. The combination provides extra resolving power for a wide variety
More informationSeparation of Macrocyclic Lactones (Avermectins) on FLARE C18 MM & FLARE C18+ Columns
Separation of Macrocyclic Lactones (Avermectins) on FLARE C8 MM & FLARE C8+ Columns Introduction Diamond Analytics Technical Note: T05- Avermectins are a series of 6-membered macrocyclic lactone derivatives
More informationPerformance characteristics of the Agilent 1100 Series preparative pump. Technical Note. Abstract
Performance characteristics of the Agilent Series preparative pump Technical Note Abstract The Agilent Series preparative pump as part of the Agilent Series purification system is especially designed for
More informationQuantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System
Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System Marta Kozak Clinical Research Applications Group Thermo Fisher Scientific San Jose CA Clinical Research use only, Not for
More informationLC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell 120 EC C18 Column
Application Note Clinical Research LC/MS/MS Separation of Cholesterol and Related Sterols in Plasma on an Agilent InfinityLab Poroshell EC C8 Column Authors Rongjie Fu and Zhiming Zhang Agilent Technologies
More informationAchiral SFC: Development of an Orthogonal SFC Method for Mometasone Furoate Impurity Analysis
Achiral SFC: Development of an Orthogonal SFC Method for Mometasone Furoate Impurity Analysis Zhenyu Wang Merck Research Laboratories NJCG 2013 Outline SFC chronicle The journey of a half century Re-birth
More informationEdgar Naegele. Abstract
Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in
More informationSeparation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement
Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Application Note Food Testing & Agriculture Author Edgar Naegele Agilent Technologies,
More informationFig.1. Denatonium benzoate (DB) chemical structure
ILIADE code 280 CLEN Method Determination of Denatonium Benzoate in Alcoholic Products by HPLC-UV 1 Scope The purpose of this method is verification of fulfilment of the legislative requirements on denatured
More informationReduced Ion Suppression and Improved LC/MS Sensitivity with Agilent Bond Elut Plexa
Reduced Ion Suppression and Improved LC/MS Sensitivity with Agilent Bond Elut Plexa Application Note Small Molecule Pharmaceuticals & Generics Author Mike Chang Agilent Technologies, Inc. 5 Commercentre
More informationShuguang Li, Jason Anspach, Sky Countryman, and Erica Pike Phenomenex, Inc., 411 Madrid Ave., Torrance, CA USA PO _W
Simple, Fast and Accurate Quantitation of Human Plasma Vitamins and Their Metabolites by Protein Precipitation Combined with Columns Using HPLC-UV, HPLC-FLD or LC/MS/MS Shuguang Li, Jason Anspach, Sky
More informationRoc On with These Dependable LC Columns
Roc LC s Conventional column built to be the cornerstone for your lab; pressure rated for any 400 bar system. Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you
More informationAPPLICATIONS TN µm Core-Shell Particle. (continued on page 2) Page 1 of 8. For additional technical notes, visit
Kinetex 5 µm Core-Shell Columns Deliver Significant Improvements in Chromatographic Efficiency and Resolution versus Traditional Fully Porous 5 µm Columns Philip J. Koerner and Jeff Layne Phenomenex, Inc.,
More informationApplication Note. Separation of clindamycin phosphate and process impurities. Summary. Introduction. Category Pharmaceutical analysis Matrix
Application Note Separation of clindamycin phosphate and process impurities Category Pharmaceutical analysis Matrix Drugs Method UHPLC Keywords Antibiotics, process impurities, quality control Analytes
More informationA Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS
A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS By Shun-Hsin Liang and Frances Carroll Abstract Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these
More informationC30 ISOMERS HAVE MET THEIR MATCH
C30 ISOMERS HAVE MET THEIR MATCH INTRODUCING THE NEW! Built on proven Fused-Core particle technology, the is designed to deliver fast separations ideal for lipids and isomers compared to your C18. FEATURES
More informationHigh Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column
High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column Application Note BioPharma Authors James Martosella, Phu Duong, and Alex Zhu Agilent Technologies, Inc. Abstract
More informationAnalysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column
Application Note Pharmaceutical and Food Testing Analysis of Amino Acids Derived Online Using an Agilent AdvanceBio AAA Column Author Lu Yufei Agilent Technologies, Inc. Abstract A liquid chromatographic
More informationBarry Boyes 1,2, Shujuan Tao 2, and Ron Orlando 2
Barry Boyes 1,2, Shujuan Tao 2, and Ron Orlando 2 1 Advanced Materials Technology, Inc. Wilmington, DE USA 2 Complex Carbohydrate Research Center University of Georgia, Athens, GA USA bboyes@advanced-materials-tech.com
More informationCore-Shell Technology for Proteins and Peptides
Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein
More informationAgilent Technologies Prep LC Columns
Agilent Technologies Prep LC Columns Agilent Technologies Prep LC Columns Agilent Technologies has always taken seriously its responsibility to ensure your success. That s why all our instruments and supplies
More informationAnalysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector
application Note UHPLC Author Njies Pedjie PerkinElmer, Inc. Shelton, CT 06484 USA Analysis of Isoflavones with the PerkinElmer Flexar FX-15 UHPLC System Equipped with a PDA Detector Introduction Foods
More informationThis revision also necessitates a change in the table numbering in the test for Organic Impurities.
Methylphenidate Hydrochloride Extended-Release Tablets Type of Posting Notice of Intent to Revise Posting Date 27 Jul 2018 Targeted Official Date To Be Determined, Revision Bulletin Expert Committee Chemical
More informationDevelopment and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets
Journal of PharmaSciTech 0; ():- Research Article Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets * Sayyed Hussain,
More informationAgilent 1220 Infinity LC System Performance Specifications
IET International Equipment Trading, Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Agilent 1220 Infinity LC System Performance
More informationAgilent 1260 Infinity Analytical SFC System
Agilent 1260 Infinity Analytical SFC System Installation Instructions: Checkout Procedure This technical note describes the checkout procedures for the Agilent 1260 Infinity Analytical SFC System. Contents
More informationZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006)
September 2006 RESTRICTED ZIDOVUDINE, LAMIVUDINE AND ABACAVIR TABLETS Draft proposal for The International Pharmacopoeia (September 2006) This document was provided by a contracted quality control laboratory.
More informationChoosing Preparative Columns
Choosing Preparative Relationship between Column I.D., Sample Loading Volume and Flow Rate In preparative operations, column internal diameter sizes from 6.0 to 100 mm are widely used. When the analytical
More informationSEDEX 90LT Presentation
SEDEX 90LT Presentation HPLC 2011, Budapest SENSITIVITY FLEXIBILITY SEDERE EXPERIENCE SEDEX 90LT Technology SEDEX 990LT 0LT Light Source Selected Blue-Violet Laser 405nm 10mW Output SEDEX 90LT Technology
More informationAnalysis of Common Sweeteners and Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector
application Note Liquid Chromatography Author Njies Pedjie PerkinElmer, Inc. Shelton, CT 06484 USA Analysis of Common Sweeteners and Additives in Beverages with the PerkinElmer Flexar FX-15 System Equipped
More informationSeparation of Vitamin D and Vitamin D Metabolites on FLARE C18 MM (Mixed Mode) HPLC Column
Diamond Analytics Technical Note: T131-1 Separation of Vitamin D and Vitamin D Metabolites on FLARE C1 MM (Mixed Mode) HPLC Column Introduction In this technical note, the versatility of the diamond-based
More informationAnalysis of Counterfeit Antidiabetic Drugs by UHPLC with the Agilent 1220 Infinity Mobile LC
Analysis of Counterfeit Antidiabetic Drugs by UPLC with the Agilent 1 Infinity Mobile LC Application Note Small Molecule Pharmaceuticals & Generics Author Sonja Schneider Agilent Technologies, Inc. Waldbronn,
More informationRebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica
Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica This
More informationRobust extraction, separation, and quantitation of structural isomer steroids from human plasma by SPE-UHPLC-MS/MS
TECHNICAL NOTE 21882 Robust extraction, separation, and quantitation of structural isomer steroids human plasma by SPE-UHPLC-MS/MS Authors Jon Bardsley 1, Kean Woodmansey 1, and Stacy Tremintin 2 1 Thermo
More informationProfiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography
Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Application Note Metabolomics Author Yuqin Dai Agilent Technologies, Inc. 31 Stevens Creek Blvd,
More informationIdentification of Steroids in Water by Ion Trap LC/MS/MS Application
Identification of Steroids in Water by Ion Trap LC/MS/MS Application Environmental Author Paul Zavitsanos and Christine Miller Agilent Technologies, Inc. Centerville Road Wilmington, DE 9- USA Abstract
More informationRP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form
RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form S. LAVANYA* 1, SK. MANSURA BEGUM 1, K. NAGAMALLESWARA RAO 2, K. GAYATHRI DEVI 3 Department of pharmaceutical
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationAnalytical and Preparative SFC Columns
Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...
More information