Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors

Transcription

1 Analytical and Bioanalytical Chemistry Electronic Supplementary Material Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors Zhicheng Liu, Peiyuan Yin, Roland Amathieu, Philippe Savarin, Guowang Xu S-1

2 Table of Contents Table S1 Identified metabolites from the retained peaks in the pre-processing with the help of the database. Page S-3 to Page S-7 Table S2 Determined varied metabolites in the comparison between the survivors and the non-survivors. Page S8 topage S-9 Figure S1 PCA score plot for all the QC samples and real samples Page S-10 Figure S2 Validation of the PLS-DA model for the separation between the survivors and the non-survivors in the negative mode. Page S-10 Figure S3 Boxchart for the ratio of carnitine C 2:0 and carnitine for both the survivors and the non-survivors. Page S-11 S-2

3 Table S1 Identified metabolites from the retained peaks following the pre-processing with the help of retention time and database. *: metabolites with VIP > 1; #: metabolites with p-value < 0.05; ^: metabolites confirmed by the MS/MS; metabolites identified by the database but rejected by the comparison to the result of MS/MS; &: significantly varied metabolites defined by both the positive and negative modes M/Z Mode Time Attribution Positive 0.44 Pyridine Positive 0.66 DL-Alanine Positive 0.91 L-Alanine?*# Positive 0.41 L-Alanine^ Positive 0.44 Amino-isobutric acid*# Positive 0.4 Choline Positive ,(4)-Hydroxybutryric acid*# Positive 2.86 Uracil*# Positive 0.69 Creatinine?*# Positive 0.42 Creatinine*#^ Positive 0.96 DL-proline*# Positive 0.71 L-Proline*#^ Positive 0.44 Betaine*#^ Positive 0.44 L-Valine*#^ Positive 0.72 L-Valine?*# Positive 0.44 L-Threonine Positive 3.1 Phenylethylamine* Positive 0.68 Pyroglutamic acid Positive 0.44 Pipecolic acid Positive 0.43 Creatine*#^ Positive 0.71 Creatine?*# Positive 1.11 L-isoleucine*#^ Positive 0.84 L-Leucine*#^ Positive 0.35 Ornithine*#^ Positive 0.64 Hypoxanthine*#^ Positive 2.52 Aminobenzoic acid* Positive 0.44 Urocanic acid Positive 4.85 Octenoic acid Positive 0.71 Proline betaine*# Positive 2.15 Acetylcholine* Positive 0.71 DL-Glutamine? Positive 0.44 L-Glutamine*#^& Positive 0.35 L-Lysine^ Positive 0.44 L-Glutamate*#^ Positive 0.62 Methionine^ Positive 0.71 PhenylGlycine Positive 0.34 Methylcysteine sulfoxide^ S-3

4 Positive 0.67 Xanthine Positive 0.71 L-carnitine Positive 0.95 Methionine sulfoxide^ Positive 1.85 DL-Phenylalanine Positive 1.57 L-Phenylalanine*#^& Positive 1.32 DL-Phenylalanine?*# Positive 0.59 Uric acid Positive 4.28 Indoleacetic Acid^ Positive 4.7 Indoleacetic Acid? Positive 1.83 Citrulline*#^ Positive 3.52 Citrulline?*# Positive 2.85 Citrulline?*# Positive 1.83 Hippuric acid*#^ Positive 2.56 Glucose Positive 2.28 L-Tyrosine? Positive 1.1 Tyrosine?*# Positive 0.71 L-Tyrosine*#^& Positive 0.41 Epinephrine Positive 2.43 L-Tryptophan*# Positive Methylhippuric acid*#^ Positive 3.07 Caffeine^ Positive 3.56 Caffeine? Positive 2.96 Salicyluric acid Positive 0.62 Carnitine C2:0*#^ Positive 4.31 N-acetyl-DL-Phenylalanine Positive 1.06 Carnitine C3: Positive 1.99 N-acetyl-L-tyrosine* Positive 1.01 Ile-Pro* Positive 0.76 Pro-Leu Positive 2.33 Carnitine C4:0*#^ Positive 3.05 Carnitine C5: Positive 4.52 N-acetyl-DL-Tryptophan Positive 0.45 Deoxyadenosine Positive 0.68 Ribothymidine Positive 4.17 Carnitine C6:0? Positive 4.44 Carnitine C6:0*#^ Positive 2.76 Glu-Leu* Positive 0.63 Epidermin Positive 4.28 Leucyl-Phe Positive 0.63 Glycated valine Positive 2.98 α-hydroxymetoprolol*# Positive 4.99 Carnitine C8:1*# Positive 5.16 Carnitine C8:0*# Positive 8 Sphinganine S-4

5 Positive 1.28 Glycated Isoleucine Positive 0.98 Glycated leucine Positive 11.8 Sphingosine Positive 4.5 Phe-Phe Positive 6.37 Carinitine C10: Positive 6.86 Carnitine C10:0*#^ Positive 1.32 p-acetamidophenyl glucuronide Positive MG 16:0* Positive 4.72 Nicotine glucuronide* Positive 7.47 Carnitine C12: Positive 7.93 Carnitine C12:0*#^ Positive MG 18:0^ Positive 7.91 Carnitine C14: Positive 8.44 Carnitine C14: Positive Hydroxy-3,5-bis(1-methylethyl)phenyl glucuronide Positive 8.97 Carnitine C14:0^ Positive 5.83 Terazosin Positive 9.32 Carnitine C16:1* Positive 9.91 Carnitine C16:0*#^ Positive Ketocholesterol Positive 9.91 Calcifediol Positive Carnitine C18: Positive Carnitine C18:0*#^ Negative 0.74 Propionic acid* Negative 0.61 L-Lactic acid*#^ Negative 1.75 DL-Lactic acid?*# Negative 1.21 DL-Lactic acid?*# Negative Oxobutanoate Negative 1.04 Hydroxybutyric acid Negative 1.54 Hydroxyisobutyric acid Negative 0.43 Glyceric acid Negative 0.76 Succinic acid*#^ Negative 2.31 Hydroxyvaleric acid*# Negative 3.19 Benzoic acid Negative 0.68 DL-Glutamate* Negative 0.78 DL-Leucine/Isoleucine Negative 0.41 Malic acid*#^ Negative 0.43 Threonic acid* Negative 4.48 Salicylic acid Negative 2.32 Adipic acid Negative 0.4 L-Glutamine*#^& Negative Hydroxy enanthoic acid Negative 0.43 L-Glutamate S-5

6 Negative 0.79 alpha-ketoglutarate*#^ Negative 3.85 Cinnamic acid*# Negative 0.43 Ribose Negative Phenylglycine Negative 0.77 Xanthine* Negative 3.54 p-hydroxyphenylacetic acid Negative 2.5 Succinylacetone*# Negative 3.5 N-isovalerylglycine Negative 1.89 L-Phenylalanine*#^& Negative 2.2 Phenol sulfate*# Negative 0.4 Allo-Inostiol* Negative 0.74 L-Tyrosine*#^& Negative 7.12 FFA C11: Negative 6.94 OH-FFA C11:1* Negative 3.38 p-cresol sulfate Negative 4.7 Nonanedioic acid Negative 0.65 Citric acid*#^ Negative 0.43 Quinic acid Negative 5.44 Sebacic acid* Negative 0.76 Acetylcarnitine Negative Hydroxymethylantipyrine Negative 2.43 L-Tryptophan Negative 4.12 Indolelactic acid*#^ Negative 3.55 Kynurenine Negative 3.53 Hydroxyphenylacetylglycine Negative 2.49 Indoxy sulfate*#^ Negative 4.95 Methylthiobenzoic acid Negative 8.62 Hydroxydodecanoic acid*# Negative 1.72 Acetyl-L-tyrosine*^ Negative 2.64 Acetyl-L-tyrosine?*# Negative 6.34 Traumatic acid Negative 6.81 Dodecanedioic acid Negative 1.47 Norepinephrine sulfate Negative 3.53 Leu-Iso Negative 5.51 Asp-Asp Negative 9.82 FFA 16:0*#^ Negative 3.18 Phe-Gln Negative FFA 17: Negative FFA 18:0*#^ Negative 3.2 Argininosuccinate*#^ Negative 7.17 FFA 22: Negative 6.36 Etiocholanolone sulfate Negative 5.78 DHAS Negative 6.83 ANDS S-6

7 Negative 9.27 UDCA*#^ Negative 12 CDCA Negative 7.84 CA^ Negative 11.1 LPC 16:0^ Negative LPC 18: Negative 7.92 GUDCA*#^ Negative 6.99 GCDCA*#^ Negative 8.92 LPE 16: Negative 5.91 GCA*#^ Negative 7 LPC 14:0*#^ Negative 9.64 LPE 18:2*#^ Negative LPE 18: Negative LPE 18:0*#^ Negative 9.15 LPE 20:5*#^ Negative 6.31 TCDCA Negative 9.7 LPE 20:4*#^ Negative LPE 20:3*# Negative 6.4 TCA Negative 6.8 GUDCS*#^ S-7

8 Table S2 Discriminating metabolites in the comparison between septic survivors and non-survivors. The fold change is calculated by the report of concentration survivor/non-survivor, thereby values which are inferior to 1 are for decreased metabolites in dead patients and increased metabolites possess values which are superior to 1 Identified Metabolites Mode m/z tr (Min) P-Value Fold change L-Lactic acid Negative E Creatinine Positive E L-Proline Positive E Succinic acid Negative E Betaine Positive E L-Valine Positive E Creatine Positive E L-Leucine Positive E L-Isoleucine Positive E Malic acid Negative E Ornithine Positive E Hypoxanthine Positive E L-Glutamine Negative E alpha-kg Negative E L-Glutamate Positive E L-Phenylalanine Positive E Citrulline Positive E Hippuric acid Positive E L-Tyrosine Positive E Citric acid Negative E Methylhippuric acid Positive E Indolelactic acid Negative E Carnitine C2:0 Positive E Indoxy sulfate Negative E Carnitine C4:0 Positive E FFA 16:0 Negative E Carnitine C6:0 Positive E FFA 18:0 Negative E Argininosuccinate Negative E Carnitine C10:0 Positive E Carnitine C12:0 Positive E UDCA Negative E Carnitine C16:0 Positive E Carnitine C18:0 Positive E GUDCA Negative E GCDCA Negative E GCA Negative E S-8

9 LPC 14:0 Negative E LPE 18:2 Negative E LPE 18:0 Negative E LPE 20:5 Negative E LPE 20:4 Negative E GUDCS Negative E S-9

10 Fig. S1 PCA score plot for all the real samples and QC samples. Red triangles: QC samples; blue squares : real samples Fig. S2 Validate plot of the PLS-DA model revealing that the model is not over-fitting. The samples were permutated into a different orders from those in the established model for 200 times. The green triangles stand for the obtained R 2 value (goodness of fit) and the blue squares stand for the obtained Q 2 value (representing the predictive power of the model with a certain number of component) by the 200 permutations. The Y-axis represents R 2 and Q 2 value for every model while the X-axis represents the correlation coefficient between original and permuted response data. For a model not over-fitting, it is necessary that the two curve have their positive slope S-10

11 Fig. S3 Boxchart for the comparison of the ratio of carnitine C 2:0 / carnitine in serum between septic shock non-survivors and survivors. The lower level of the ratio implies a faster beta-oxidation in the patient S-11