SecurityGuard. Column Protection for UHPLC, HPLC to PREP. A Universal Guard Cartridge System. Increases HPLC Column Lifetime, Guaranteed!

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1 U.S. Patent No. 6, 162, 362 Column Protection for UHPLC, HPLC to PREP Protect HPLC columns and extend lifetime Virtually no change in chromatography Available in analytical, semi-prep, and preparative sizes Simple to use Did you know a common cause of high backpressure, split pks, broad pks, baseline noise, baseline drift and loss of resolution is contaminants? The fact is all mobile phases contain some chemical contaminants or microparticulates, from the sample, solvent, or wr on the polymeric sls of the pump or injector. These contaminants can clog frits, irreversibly bind to an HPLC column, degrade performance, and even damage the flow cell. An sy solution, SecurityGuard is a universal HPLC guard cartridge system designed to effectively (and inexpensively), protect your valuable columns, from the damaging effects of chemical contaminants, without altering your chromatographic results. A Universal Guard System How SecurityGuard Works* The traditional SecurityGuard analytical cartridge holder (patented) directly finger-tightens into virtually any manufacturer s non core-shell and 3 μm particle columns. Contaminants are retained by an inexpensive, 4 mm, disposable cartridge instd of fouling your expensive analytical column. contaminants compounds of interest FLOW From injector Cutaway view showing cartridge can be sily inspected for contaminants Guard Universal Fingertight connection to HPLC column no wrenches required If the SecurityGuard System does not provide at lst an equivalent performance as compared to a competing guard cartridge system, return the product with the comparative data within 45 days for a FULL REFUND. Fingertight connection to virtually any female inverted endfitting worldwide* See it in action video: To detector Incrses HPLC Column Lifetime, Guaranteed! Simply replace SecurityGuard cartridges instd of your expensive HPLC columns. In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use HPLC column Replaced HPLC SECURITYGUARD Backpressure (Bar) For available Semi-Preparative and PREP sizes, see pp For UHPLC, core-shell, and / or < 3 μm particle column protection, see SecurityGuard ULTRA p * Fture applies to traditional analytical-sized guard system only, and does not apply to SemiPrep or PREP guard cartridges. -1 Column returns to initial backpressure Injections Accelerated lifetime test using endogenous biomolecule matrix on a reversed phase C18 column, 5 µm, 5 x 4.6 mm with SecurityGuard C18 cartridges. Backpressure values represent additional backpressure contributed by SecurityGuard. 251

2 U.S. Patent No. 6, 162, 362 See Your Dirt Fture The see your dirt fture lets you know exactly when it s time to replace your cartridge. Visually inspect the surface of the cartridge s packing material any time, without disturbing the packing bed. Now you can sily monitor visual contaminant build-up, and change your guard cartridge before it s too late! If your contaminants are colorless, replace the cartridge as often as needed to maintain chromatographic performance. Compare, Virtually No Change mau Without SecurityGuard App ID 1731 CLEAN If it looks cln, the cartridge may be reinserted for further use. DIRTY If either discoloration or particle build-up is observed, it s time to replace the cartridge. mau min vs. With SecurityGuard min App ID 1731 SECURITYGUARD HPLC The SecurityGuard is sy to use and cartridge replacement is simple. F. Shakir, Sheffield Pharmaceuticals We didn t see any change in retention time or difference in the pks. The SecurityGuard has incrsed the life of the column. B. Dietz, ADM The opinions stated herein are solely those of the individual and not necessarily those of any company or organization. Columns: As specified within App ID Dimensions: 15 x 4.6 mm Guard : SecurityGuard C18 (ODS) 4 x 3. mm ID Mobile Phase:.1 M Ammonium acetate/acetonitrile/methanol (35:25:4) Flow Rate:.6 ml/min Detection: 245 nm Sample: 1. Clonazepam 2. Oxazepam 3. Diazepam 4. Bromazepam 5. Temazepam 6. Medazepam Analytical Holder Kit and Accessories For 2. and 3. mm ID cartridges, use with 2. to 8. mm ID columns Analytical Kit Part No. Description Price KJ-4282 SecurityGuard Kit* (includes holder) *Kit KJ-4282 Includes: PEEK Ferrules (Qty. 3) Replacement Parts and Accessories AJ-4283 PEEK Ferrules 3/pk AJ-4285 Stacking Rings AQ-1389 PEEK Fingertight Fittings 1/pk AJ-4284 SecurityGuard Wrenches Holder (Qty. 1) Stacking Rings (to stack 2 cartridges for extra protection) (Qty. 2) Wrenches (Qty. 2) PEEK Fingertight Fittings (Qty. 2) 252

3 Semi-Preparative Holder For 1. mm ID cartridges, use with 9 to 16 mm ID columns SemiPrep Guard Holder AJ-722 Holder for 1. mm ID cartridges Accessories Nut and Ferrule AQ Thrded Male Nut and Ferrule Set for 1/16 in. OD capillary tubing Sure-Lok Fingertight Fittings AQ-1388 PEEK Sure-Lok Male Nut AQ-1389 PEEK Sure-Lok Male Nut 1/pk Sure-Lok Couplers AQ-1392 PEEK Sure-Lok Coupler AQ-1393 PEEK Sure-Lok Coupler 1/pk Column Sling Plugs AQ-217 Column Sling Plug, 1-32 Thrd size 1/pk Holder (1 x 1 mm ID) For Semi-Preparative and Preparative s, see p. 254 SecurityGuard PREP PREP HPLC Column Preparative Holder (Two Sizes) For 21.2 mm ID cartridges, use with 18 to 29 mm ID columns Prep Guard Holder AJ-8223 HPLC Holder Kit for 21.2 mm ID cartridges, AJ-8617 SFC Holder Kit for 21.2 mm ID cartridges, For 3. mm ID cartridges, use with 3 to 49 mm ID columns Prep Guard Holder AJ-8277 HPLC Holder Kit for 3. mm ID cartridges, AJ-8618 SFC Holder Kit for 3. mm ID cartridges, PREP 21.2 mm ID HPLC Holder Holders SFC 21.2 mm ID SFC Holder s (15 x 21.2 mm ID) HPLC SECURITYGUARD Replacement Parts and Accessories AQ-8374 PREP Coupler, SS w / PEEK Ferrule Inserts, 1-32 Thrds, 1 /16 in. OD x.2 in. ID AQ-8375 Replacement Ferrule Inserts, for PREP Coupler, 1/pk PEEK,.2 in. ID AQ-8222 PREP Replacement, Kalrez For 15 x 21.2 mm SG HPLC Holder, Size 2-21 AQ-8318 PREP Replacement, Kalrez For 15 x 3 mm SG HPLC Holder, Size 2-25 AQ-85 PREP Replacement, Teflon For 15 x 21.2 mm SG SFC Holder, Size 2-21 AQ-851 PREP Replacement, Teflon For 15 x 3 mm SG SFC Holder, Size 2-25 AT-465 Capillary S.S. Tubing,.2 in. ID x.62 in. 5/pk ( 1 /16 in.) OD x 1 cm length AT-466 Capillary S.S. Tubing,.2 in. ID x.62 in. ( 1 /16 in.) OD x 2 cm length 5/pk 3 mm ID HPLC Holder Kalrez 3 mm ID SFC Holder Teflon (15 x 3. mm ID) Coupler PREP Coupler 253

4 s and Holders Step 1: Choose column ID Step 2: Match column phase If the SecurityGuard System does not provide at lst an equivalent performance as compared to a competing guard cartridge system, return the product with the comparative data within 45 days for a FULL REFUND. Choose your HPLC Column Internal Diameter, ID (mm): Analytical SemiPrep PREP HPLC or SFC Use s (mm): 4. x x 3. 1 x x x 3. SECURITYGUARD HPLC Material Description ph Stability s for General Purpose/Pharmaceutical /1pk /1pk /3pk C18 (ODS, Octadecyl) AJ-4286 AJ-4287 AJ-7221 AJ-7839 AJ-831 C12 (Dodecyl) AJ-673 AJ-674 AJ-7275 AJ-7842 AJ-834 C8 (MOS, Octyl) AJ-4289 AJ-429 AJ-7222 AJ-784 AJ-832 C5 (Pentyl) AJ-4292 AJ-4293 AJ-7372 C1 (TMS) 2-9 AJ-4298 AJ-4299 AJ-7373 Silica AJ-4347 AJ-4348 AJ-7223 AJ-7229 AJ-8312 HILIC HILIC AJ-8328 AJ-8329 AJ-892 NH 2 (Amino, Aminopropyl) AJ-431 AJ-432 AJ-7364 AJ-8162 AJ-839 CN (Cyano, Cyanopropyl) AJ-434 AJ-435 AJ-7313 AJ-822 AJ-8311 Phenyl (Phenylhexyl) AJ-435 AJ-4351 AJ-7314 AJ-7841 AJ-833 PFP(2) Pentafluorophenyl AJ-8326 AJ-8327 AJ-8376 AJ-8377 AJ-8378 SCX (SA, Strong Cation Exchanger) AJ-437 AJ-438 AJ-7369 AJ-8595 AJ-8596 SAX (SB, Strong Anion Exchanger) AJ-431 AJ-4311 AJ-737 RP-1 (Reversed Phase - Polymer) - 14 AJ-588 AJ-589 AJ-7368 AJ-8358 Polar-RP (Ether-linked Phenyl) AJ-675 AJ-676 AJ-7276 AJ-7845 AJ-837 Fusion-RP (C18 Polar Embedded) AJ-7556 AJ-7557 AJ-7558 AJ-7844 AJ-836 AQ C18 (Polar Endcapped C18) AJ-751 AJ-7511 AJ-7512 AJ-7843 AJ-835 Gemini NX-C18 (C18 TWIN-NX Technology) 1-12 AJ-8367 AJ-8368 AJ-8369 AJ-837 AJ-8371 Gemini C18 (C18 TWIN Technology) 1-12 AJ-7596 AJ-7597 AJ-7598 AJ-7846 AJ-838 Gemini C6-Phenyl (C6-Phenyl TWIN Technology) 1-12 AJ-7914 AJ-7915 AJ-9156 AJ-9157 AJ-9158 Oligo-RP (C18 TWIN Technology) 1-12 AJ-8134 AJ-8135 AJ-8136 AJ-821 AJ-831 Oligo-WAX (WA, Wk Anion Exchanger) AJ-8324 AJ-8325 AJ-8639 AJ-842 s for Protein and Polypeptide Reversed Phase /1pk /1pk /3pk For use with silica columns for separation of proteins & peptides, such as Jupiter (); Vydac 218TP, 214TP (Alltech Associates, Inc.); Nucleosil 3 Å C18, C4 (Macherey-Nagel);Hypersil 3 Å, (Thermo Hypersil-Keystone), and other widepore or 3 Å brands. Widepore C18 (ODS, Octadecyl) AJ-432 AJ-4321 AJ-7224 AJ-723 AJ-8313 Widepore C5 (Pentyl) AJ-4326 AJ-4327 AJ-7371 Widepore C4 (Butyl) AJ-4329 AJ-433 AJ-7225 AJ-7231 AJ-8314 s for Silica GFC /1pk (Aqueous SEC) For use with silica GFC columns, such as Yarra and BioSep (); ZORBAX GF-Series (Agilent); Bio-Sil (Bio-Rad). GFC AJ-4487 AJ-8588 GFC AJ-4488 AJ-8589 GFC AJ-4489 AJ-859 s for Chiral /1pk /1pk /3pk For use with chiral columns, such as Lux Cellulose-1, -2, -3, -4, & Amylose-2 (); CHIRALCEL OD-H, CHIRALCEL OJ-H & CHIRALPAK AD -H (DAICEL Corporation) Lux Cellulose-1 Cellulose tris (3, 5-di 2-9 AJ-842 AJ-843 AJ-844 AJ-845 AJ-846 Lux Cellulose-2 Cellulose tris (3-chloro AJ-8398 AJ-8366 AJ-8399 AJ-84 AJ-841 Lux Cellulose-3 Cellulose tris (4-methylbenzoate) 2-9 AJ-8621 AJ-8622 AJ-8623 AJ-8624 AJ-8625 Lux Cellulose-4 Cellulose tris (4-chloro AJ-8626 AJ-8627 AJ-8628 AJ-8629 AJ-863 Lux Amylose-2 Amylose tris (5-chloro AJ-8471 AJ-847 AJ-8472 AJ-8473 AJ-8474 s for Carbohydrate/Organic Acid /1pk For organic acid and carbohydrate analysis, such as Rezex (); Aminex (Bio-Rad); Sugar-Pak (Waters). Carbo-H AJ-449 Carbo-Ag +* Neutral AJ-4491 Carbo-Pb +2 Neutral AJ-4492 Carbo-Ca +2 Neutral AJ-4493 Guard Holders (one-time purchase only) /kit /holder /kit /kit KJ-4282 AJ-722 AJ-8223 AJ-8277 *For use with saccharide and oligosaccharide columns in Ag + form. Traditional SecurityGuard cartridges are pressure rated to 35 psi (241 bar). For UHPLC, core-shell, and / or < 3 μm particle columns and all applications at higher pressures, use SecurityGuard ULTRA, see p For preparative SFC applications, use holder AJ-8617 for 15 x 21.2 mm cartridges or AJ-8618 for 15 x 3 mm cartridges. For Kinetex Core-Shell SecurityGuard PREP cartridges, see p

5 ULTRA UHPLC Column Protection Extends HPLC, core-shell, and < 3 µm particle column lifetime Virtually no change in chromatography Fits virtually all manufacturers columns 2.1 to 4.6 mm ID Pressure rated to 2 psi (1378 bar) Simple to use Universal Fit Use SecurityGuard ULTRA with virtually all UHPLC columns 2.1 to 4.6 mm ID. The extremely low dd volume of this unique product minimizes sample pk dispersion. It will efficiently remove microparticulates and chemical contaminants from the flow strm without contributing to system backpressure or dd volume (<.3 μl). SecurityGuard ULTRA Incrses Column Lifetime, Guaranteed! When contaminates and particulates build up at the hd of your column or on the guard cartridge, system backpressures can incrse dramatically. By simply replacing the SecurityGuard ULTRA cartridge, instd of your column, you are able to regain normal operating conditions and reclaim original column performance. Accelerated lifetime test using endogenous biological matrix on Kinetex 2.6 µm C18 5 x 4.6 mm ID column Backpressure (Bar) min WITHOUT SecurityGuard ULTRA WITH SecurityGuard ULTRA 2 SecurityGuard ULTRA Performance Replacement No Guard Injection No. 3 Replacement With SecurityGuard ULTRA Protects with No Loss of Column Performance! SecurityGuard ULTRA s unique design minimizes sample pk dispersion to maintain column performance without altering your chromatography results. Conditions for both columns: Column: Kinetex 1.7 µm XB-C18 Sample: 1. Uracil Dimensions: 5 x 2.1 mm 2. Acetophenone Guard : SecurityGuard ULTRA C18 (ODS) 2.1 mm ID 3. Toluene Part No.: AJ Naphthalene Mobile Phase: Acetonitrile / Water (5:5) Flow Rate:.5 ml/min Detection: 254 nm 4 App ID App ID 1986 SecurityGuard ULTRA Holder AJ-9 SecurityGuard ULTRA Holder SecurityGuard ULTRA s UHPLC / HPLC Sure-Lok High Pressure PEEK Male Nut Fittings AQ-853 Sure-Lok High Pressure PEEK 1-Pc Nut, 1-32, for 1/16 in. Tubing, Pressure Rated to 12 psi (827 bar) 1/pk AQ-853 UHPLC Column Protection Holder Holder with cartridge, assembled s for General Purpose/ Pharmaceutical Column ID (mm) Material Description ph Stability /3pk /3pk /3pk C18 (ODS, Octadecyl) * AJ-8782 AJ-8775 AJ-8768 C8 (MOS, Octyl) * AJ-8784 AJ-8777 AJ-877 PFP (Pentafluorophenyl) * AJ-8787 AJ-878 AJ-8773 HILIC HILIC AJ-8786 AJ-8779 AJ-8772 Phenyl (Phenylhexyl) * AJ-8788 AJ-8781 AJ-8774 s for Protein and Peptide Reversed Phase Widepore C18 (ODS, Octadecyl) * AJ-8783 AJ-8769 Widepore C8 (MOS, Octyl) * AJ-8785 AJ-8771 Widepore C4 (Butyl) * AJ-8899 AJ-891 Peptide C18 (ODS, Octadecyl) * AJ-8948 AJ-8946 s for Synthetic DNA / RNA Analysis Oligo-MS C18 (ODS, Octadecyl) * AJ-968 AJ-967 AJ-966 *ph stable under gradient conditions. ph stable under isocratic conditions. Initial SecurityGuard ULTRA installation onto Kinetex, Aeris, and Oligo-MS columns and later cartridge replacement, requires 3 wrenches, which must be purchased separately: one 3/8 in. wrench (AQ-8959; fits Kinetex, Aeris, and Oligo-MS column end-fittings), and two 5/16 in. wrenches (AQ-893; fits ULTRA cartridge and holder). See p. 376 Sure-Lok Fitting Tightening Tool, Aluminum, for AQO-853 If SecurityGuard ULTRA cartridge protection system does not perform as well or better than your current guard cartridge system of similar phase and dimensions, return the product with comparative data within 45 days for a FULL REFUND. For UHPLC / HPLC High Pressure Stainless Steel Male Nut Fittings, see p. 368 A Core-Shell Performance Enhancement Kit for UHPLC is available, see p R&D 1 Award Recipient See it in action: HPLC SECURITYGUARD ULTRA 255