A Tour of the Cell. Chapter 6. Biology Eighth Edition Neil Campbell and Jane Reece. PowerPoint Lecture Presentations for

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1 Chapter 6 1 A Tour of the Cell PowerPoint Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp

2 Overview: The Fundamental Units of Life 2 All organisms are made of cells The cell is the simplest collection of matter that can live Cell structure is correlated to cellular function All cells are related by their descent from earlier cells

3 Microscopy 3 In a light microscope (LM), visible light passes through a specimen and then through glass lenses, which magnify the image The quality of an image depends on Magnification, the ratio of an object s image size to its real size Resolution, the measure of the clarity of the image, or the minimum distance of two distinguishable points Contrast, visible differences in parts of the sample

4 Electron microscope Light microscope Unaided eye LMs can magnify effectively to about 1,000 times the size of the actual specimen 10 m 1 m 0.1 m 1 cm 1 mm 100 µm 10 µm Human height Length of some nerve and muscle cells Chicken egg Frog egg Most plant and animal cells Nucleus 4 Most bacteria 1 µm Mitochondrion 100 nm Smallest bacteria Viruses 10 nm Ribosomes Proteins 1 nm Lipids Small molecules 0.1 nm Atoms

5 50 m Figure 6.3 Light Microscopy (LM) Brightfield (unstained specimen) Confocal Electron Microscopy (EM) Longitudinal section of cilium 5 Cross section of cilium Cilia Brightfield (stained specimen) 2 m Phase-contrast 1 m 10 m 50 m Deconvolution Scanning electron microscopy (SEM) 2 m Transmission electron microscopy (TEM) Differential-interferencecontrast (Nomarski) Super-resolution Fluorescence 10 m

6 6 Two basic types of electron microscopes (EMs) are used to study subcellular structures Scanning electron microscopes (SEMs) focus a beam of electrons onto the surface of a specimen, providing images that look 3-D Transmission electron microscopes (TEMs) focus a beam of electrons through a specimen and are used mainly to study the internal structure of cells

7 TECHNIQUE (a) Scanning electron microscopy (SEM) RESULTS Cilia 1 µm 7 (b) Transmission electron microscopy (TEM) Longitudinal section of cilium Cross section of cilium 1 µm

8 Comparing Prokaryotic and Eukaryotic Cells 8 Basic features of all cells: Plasma membrane Semifluid substance called cytosol Chromosomes (carry genes) Ribosomes (make proteins)

9 9 Prokaryotic cells are characterized by having No nucleus DNA in an unbound region called the nucleoid No membrane-bound organelles Cytoplasm bound by the plasma membrane

10 10 Fimbriae Nucleoid Ribosomes Plasma membrane Bacterial chromosome (a) A typical rod-shaped bacterium Cell wall Capsule Flagella 0.5 µm (b) A thin section through the bacterium Bacillus coagulans (TEM) A prokaryotic cell

11 11 Eukaryotic cells are characterized by having DNA in a nucleus that is bounded by a membranous nuclear envelope Membrane-bound organelles Eukaryotic cells are generally much larger than prokaryotic cells

12 12 The plasma membrane is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell The general structure of a biological membrane is a double layer of phospholipids

13 Outside of cell (a) TEM of a plasma membrane 13 Inside of cell 0.1 µm Carbohydrate side chain Hydrophilic region Hydrophobic region Hydrophilic region Phospholipid Proteins (b) Structure of the plasma membrane

14 14 The logistics of carrying out cellular metabolism sets limits on the size of cells The surface area to volume ratio of a cell is critical As the surface area increases by a factor of n 2, the volume increases by a factor of n 3 Small cells have a greater surface area relative to volume

15 Surface area increases while total volume remains constant Total surface area [Sum of the surface areas (height width) of all boxes sides number of boxes] Total volume [height width length number of boxes] Surface-to-volume (S-to-V) ratio [surface area volume]

16 Surface area increases while total volume remains constant Total surface area [Sum of the surface areas (height width) of all boxes sides number of boxes] Total volume [height width length number of boxes] Surface-to-volume (S-to-V) ratio [surface area volume] 6 1.2

17 A Panoramic View of the Eukaryotic Cell A eukaryotic cell has internal membranes that partition the cell into organelles 17 Plant and animal cells have most of the same organelles The nucleus contains most of the DNA in a eukaryotic cell Ribosomes use the information from the DNA to make proteins BioFlix: Tour Of An Animal Cell BioFlix: Tour Of A Plant Cell

18 Fig. 6-9a ENDOPLASMIC RETICULUM (ER) Rough ER Smooth ER Flagellum Nuclear envelope Nucleolus Chromatin NUCLEUS 18 Centrosome Plasma membrane CYTOSKELETON: Microfilaments Intermediate filaments Microtubules Ribosomes Microvilli Peroxisome Golgi apparatus Mitochondrion Lysosome Animal cell

19 Fig. 6-9b NUCLEUS Nuclear envelope Nucleolus Chromatin Rough endoplasmic reticulum Smooth endoplasmic reticulum 19 Ribosomes Golgi apparatus Central vacuole Microfilaments Intermediate filaments Microtubules CYTO- SKELETON Mitochondrion Peroxisome Plasma membrane Cell wall Wall of adjacent cell Plasmodesmata Chloroplast Plant cell

20 The Nucleus: Information Central 20 The nucleus contains most of the cell s genes and is usually the most conspicuous organelle The nuclear envelope encloses the nucleus, separating it from the cytoplasm The nuclear membrane is a double membrane; each membrane consists of a lipid bilayer

21 1 µm Nuclear envelope: Inner membrane Outer membrane Nucleolus Chromatin 21 Nucleus Nuclear pore Pore complex Surface of nuclear envelope 0.25 µm Ribosome Rough ER 1 µm Pore complexes (TEM) Close-up of nuclear envelope The nucleus and its envelope Nuclear lamina (TEM)

22 22 In the nucleus, DNA and proteins form genetic material called chromatin Chromatin condenses to form discrete chromosomes The nucleolus is located within the nucleus and is the site of ribosomal RNA (rrna) synthesis

23 Ribosomes: Protein Factories 23 Ribosomes are particles made of ribosomal RNA and protein Ribosomes carry out protein synthesis in two locations: In the cytosol (free ribosomes) On the outside of the endoplasmic reticulum or the nuclear envelope (bound ribosomes)

24 Cytosol 24 Endoplasmic reticulum (ER) Free ribosomes Bound ribosomes Large subunit 0.5 µm TEM showing ER and ribosomes Small subunit Diagram of a ribosome

25 The endomembrane system regulates protein traffic and performs metabolic functions in the cell Components of the endomembrane system: Nuclear envelope Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Plasma membrane These components are either continuous or connected via transfer by vesicles

26 The Endoplasmic Reticulum: Biosynthetic Factory 26 The endoplasmic reticulum (ER) accounts for more than half of the total membrane in many eukaryotic cells The ER membrane is continuous with the nuclear envelope There are two distinct regions of ER: Smooth ER, which lacks ribosomes Rough ER, with ribosomes studding its surface

27 Smooth ER Rough ER Nuclear envelope 27 ER lumen Cisternae Ribosomes Transport vesicle Smooth ER Rough ER Transitional ER 200 nm

28 Functions of Smooth ER 28 The smooth ER Synthesizes lipids Metabolizes carbohydrates Detoxifies poison Stores calcium

29 Functions of Rough ER 29 The rough ER Has bound ribosomes, which secrete glycoproteins (proteins covalently bonded to carbohydrates) Distributes transport vesicles, proteins surrounded by membranes Is a membrane factory for the cell

30 The Golgi Apparatus: Shipping and Receiving Center 30 The Golgi apparatus consists of flattened membranous sacs called cisternae Functions of the Golgi apparatus: Modifies products of the ER Manufactures certain macromolecules Sorts and packages materials into transport vesicles

31 31 cis face ( receiving side of Golgi apparatus) Cisternae 0.1 µm trans face ( shipping side of Golgi apparatus) TEM of Golgi apparatus

32 Lysosomes: Digestive Compartments A lysosome is a membranous sac of hydrolytic enzymes that can digest 32 macromolecules Animation: Lysosome Formation Lysosomal enzymes can hydrolyze proteins, fats, polysaccharides, and nucleic acids Some types of cell can engulf another cell by phagocytosis; this forms a food vacuole A lysosome fuses with the food vacuole and digests the molecules Lysosomes also use enzymes to recycle the cell s own organelles and macromolecules, a process called autophagy

33 Fig Lysosomes 33 Nucleus 1 µm Vesicle containing two damaged organelles 1 µm Mitochondrion fragment Lysosome Peroxisome fragment Lysosome Digestive enzymes Lysosome Plasma membrane Digestion Peroxisome Food vacuole Vesicle Mitochondrion Digestion (a) Phagocytosis (b) Autophagy

34 Vacuoles: Diverse Maintenance Compartments 34 A plant cell or fungal cell may have one or several vacuoles Food vacuoles are formed by phagocytosis Contractile vacuoles, found in many freshwater protists, pump excess water out of cells Central vacuoles, found in many mature plant cells, hold organic compounds and water

35 35 Central vacuole Cytosol Nucleus Central vacuole Cell wall Chloroplast 5 µm

36 Nucleus 36 Rough ER Smooth ER cis Golgi Review: relationships among organelles of the endomembrane system trans Golgi Plasma membrane

37 Mitochondria and chloroplasts change energy from one form to another 37 Mitochondria are the sites of cellular respiration, a metabolic process that generates ATP Chloroplasts, found in plants and algae, are the sites of photosynthesis Peroxisomes are oxidative organelles

38 Mitochondria and chloroplasts 38 Are not part of the endomembrane system Have a double membrane Have proteins made by free ribosomes Contain their own DNA

39 Figure 6.16 Endoplasmic reticulum Nucleus 39 Engulfing of oxygen- Nuclear using nonphotosynthetic envelope prokaryote, which becomes a mitochondrion Mitochondrion Ancestor of eukaryotic cells (host cell) Nonphotosynthetic eukaryote At least one cell Engulfing of photosynthetic prokaryote Chloroplast Mitochondrion Photosynthetic eukaryote

40 Mitochondria: Chemical Energy Conversion 40 Mitochondria are in nearly all eukaryotic cells They have a smooth outer membrane and an inner membrane folded into cristae The inner membrane creates two compartments: intermembrane space and mitochondrial matrix Cristae present a large surface area for enzymes that synthesize ATP

41 Fig Intermembrane space Outer membrane Free ribosomes in the mitochondrial matrix Inner membrane Cristae Matrix 0.1 µm

42 Chloroplasts: Capture of Light Energy 42 The chloroplast is a member of a family of organelles called plastids Chloroplasts contain the green pigment chlorophyll, as well as enzymes and other molecules that function in photosynthesis Chloroplasts are found in leaves and other green organs of plants and in algae

43 Chloroplast structure includes: Thylakoids, membranous sacs, stacked to form a granum 43 Stroma, the internal fluid Ribosomes Stroma Inner and outer membranes Granum Thylakoid 1 µm

44 Figure 6.18b m Chloroplasts (red) (b) Chloroplasts in an algal cell

45 Peroxisomes: Oxidation 45 Peroxisomes are specialized metabolic compartments bounded by a single membrane Peroxisomes produce hydrogen peroxide (H 2 O 2 ) and convert it to water 2H 2 + O 2 2H 2 O explosion! Oxygen is used to break down different types of molecules

46 The cytoskeleton is a network of fibers that organizes structures and activities in the cell The cytoskeleton is a network of fibers extending throughout the cytoplasm It organizes the cell s structures and activities, anchoring many organelles It is composed of three types of molecular structures: Microtubules Microfilaments Intermediate filaments

47 10 m Figure 6.20 The cytoskeleton 47

48 Components of the Cytoskeleton 48 Microtubules are the thickest of the three components of the cytoskeleton Microfilaments, also called actin filaments, are the thinnest components Intermediate filaments are fibers with diameters in a middle range Microtubule 0.25 µm Microfilaments

49 Roles of the Cytoskeleton: Support, Motility, and 49 Regulation The cytoskeleton helps to support the cell and maintain its shape It interacts with motor proteins to produce motility Inside the cell, vesicles can travel along monorails provided by the cytoskeleton

50 ATP Vesicle Receptor for motor protein 50 (a) Motor protein (ATP powered) Microtubule of cytoskeleton Microtubule Vesicles 0.25 µm (b)

51 Table m 10 m 5 m Column of tubulin dimers 25 nm Actin subunit Keratin proteins Fibrous subunit (keratins coiled together) Tubulin dimer 7 nm 8 12 nm

52 Microtubules 52 Functions of microtubules: Shaping the cell Guiding movement of organelles Separating chromosomes during cell division

53 53 Centrosomes and Centrioles In many cells, microtubules grow out from a centrosome near the nucleus The centrosome is a microtubule-organizing center In animal cells, the centrosome has a pair of centrioles, each with nine triplets of microtubules arranged in a ring

54 Fig Centrosome 54 Microtubule Centrioles 0.25 µm Longitudinal section of one centriole Microtubules Cross section of the other centriole

55 55 Cilia and Flagella Microtubules control the beating of cilia and flagella, locomotor appendages of some cells Cilia and flagella differ in their beating patterns Video: Chlamydomonas Video: Paramecium Cilia

56 Fig Direction of swimming 56 (a) Motion of flagella Direction of organism s movement 5 µm A comparison of the beating of flagella and cilia motion of flagella Power stroke Recovery stroke (b) Motion of cilia 15 µm

57 Cilia and flagella share a common 57 ultrastructure: A core of microtubules sheathed by the plasma membrane A basal body that anchors the cilium or flagellum A motor protein called dynein, which drives the bending movements of a cilium or flagellum Animation: Cilia and Flagella

58 58 Animation: Cilia and Flagella Right-click slide / select Play 2011 Pearson Education, Inc.

59 Fig µm Outer microtubule doublet Dynein proteins 59 Plasma membrane Central microtubule Radial spoke Microtubules Plasma membrane (b) Cross section of cilium Protein crosslinking outer doublets Basal body 0.5 µm (a) Longitudinal section of cilium 0.1 µm Triplet (c) Cross section of basal body

60 Microfilaments (Actin Filaments) 60 Microfilaments are solid rods about 7 nm in diameter, built as a twisted double chain of actin subunits The structural role of microfilaments is to bear tension, resisting pulling forces within the cell They form a 3-D network called the cortex just inside the plasma membrane to help support the cell s shape

61 61 Microfilaments that function in cellular motility contain the protein myosin in addition to actin In muscle cells, thousands of actin filaments are arranged parallel to one another Thicker filaments composed of myosin interdigitate with the thinner actin fibers

62 62 Actin filament Muscle cell Myosin filament Myosin arm (a) Myosin motors in muscle cell contraction

63 63 Localized contraction brought about by actin and myosin also drives amoeboid movement, performed by pseudopodia (cellular extensions) Cytoplasmic streaming is a circular flow of cytoplasm within cells This streaming speeds distribution of materials within the cell In plant cells, actin-myosin interactions and sol-gel transformations drive cytoplasmic streaming Video: Cytoplasmic Streaming

64 Figure 6.27 Muscle cell Actin filament Myosin filament Myosin head (a) Myosin motors in muscle cell contraction 0.5 m 64 Cortex (outer cytoplasm): gel with actin network Inner cytoplasm: sol with actin subunits 100 m Extending pseudopodium (b) Amoeboid movement Chloroplast (c) Cytoplasmic streaming in plant cells 30 m

65 Intermediate Filaments 65 Intermediate filaments range in diameter from 8 12 nanometers, larger than microfilaments but smaller than microtubules They support cell shape and fix organelles in place Intermediate filaments are more permanent cytoskeleton fixtures than the other two classes

66 Extracellular components and connections between cells help coordinate cellular activities Most cells synthesize and secrete materials that are external to the plasma membrane These extracellular structures include: Cell walls of plants The extracellular matrix (ECM) of animal cells Intercellular junctions

67 Cell Walls of Plants 67 The cell wall is an extracellular structure that distinguishes plant cells from animal cells Prokaryotes, fungi, and some protists also have cell walls The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water Plant cell walls are made of cellulose fibers embedded in other polysaccharides and protein

68 Animal cells lack cell walls but are covered by an elaborate extracellular matrix (ECM) The ECM is made up of glycoproteins such as collagen, proteoglycans, and fibronectin Extracellular matrix (ECM) of an animal 68 cell Collagen EXTRACELLULAR FLUID Proteoglycan complex Polysaccharide molecule Carbohydrates Fibronectin Core protein Integrins Plasma membrane Proteoglycan molecule Proteoglycan complex Microfilaments CYTOPLASM

69 69 Functions of the ECM: Support Adhesion Movement Regulation

70 Intercellular Junctions 70 Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact Intercellular junctions facilitate this contact There are several types of intercellular junctions Plasmodesmata Tight junctions Desmosomes Gap junctions In animal cells

71 Plasmodesmata are channels that perforate plant cell walls Through plasmodesmata, water and small solutes (and sometimes proteins and RNA) can pass from cell to cell Interior of cell Cell walls 71 Interior of cell 0.5 µm Plasmodesmata Plasma membranes Plasmodesmata between plant cells

72 Tight Junctions, Desmosomes, and Gap Junctions 72 in Animal Cells At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid Desmosomes (anchoring junctions) fasten cells together into strong sheets Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells Animation: Tight Junctions Animation: Desmosomes Animation: Gap Junctions

73 73 Tight junctions prevent fluid from moving across a layer of cells Tight junction 0.5 µm Tight junction Intermediate filaments Desmosome Gap junctions Desmosome 1 µm Space between cells Plasma membranes of adjacent cells Extracellular matrix Gap junction 0.1 µm

74 Figure 6.UN01 Nucleus 74 (ER) (Nuclear envelope)

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