BIOLOGY 103 Spring 2001 MIDTERM LAB SECTION

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1 BIOLOGY 103 Spring 2001 MIDTERM NAME KEY LAB SECTION ID# (last four digits of SS#) STUDENT PLEASE READ. Do not put yourself at a disadvantage by revealing the content of this exam to your classmates. Your grade will be determined relative to the overall performance of the class and you may end up paying a heavy price if you provide information that may benefit those students taking the exam after you. By writing your name in this page you agree not to discuss the content of this exam with students who have not taken the exam. Doing so will not only affect your grade but also will result in disciplinary action against you. SELECT THE BEST POSSIBLE ANSWER. (3 points each) 1) An enzyme that phosphorylates a protein is known as a. pyruvate b. kinase c. phosphatase d. carrier e. channel 2) Exposure of a cell to a temperature lower than normal would cause the membrane to become less fluid because a. all protein channels cease working b. all protein carriers cease working c. all fatty acids become saturated d. more cholesterol gets deposited in the membrane e. phospholipids loose their amphipathic properties 3) The transmembrane region of an ion channel forms an aqueous pore across the lipid bilayer. Which of the following amino acids would you expect to find forming the inside of the pore? a. Leucine and Aspartic Acid b. Glycine and Serine c. Tryptophan and Methionine d. Alanine and Isoleucine e. Cysteine and Valine f. Aspartic Acid and Arginine 4) In one sentence, explain your choice of the answer for question 3. Both amino acids have (charged) polar side chains 1

2 5) A pure lipid bilayer (with no proteins) exhibits which of the following properties? a. membrane potential b. simple diffusion c. active transport d. passive transport e. all of the above 6) This molecule acts as the last electron acceptor of the electron transport chain a. NADH b. ubiquinone c. cytochrome c d. oxygen e. glucose f. ATP 7) In reference to your answer to question 6, how does that molecule enters the mitochondria? a. simple diffusion b. passive transport c. active transport d. as pyruvate e. it does not have to enter the mitochondria because it is produced there 8) In a phospholipid, fatty acids are linked to this molecule a. phosphate b. serine c. cholesterol d. glycerol e. isoprene f. both (a) and (d) are correct 9) Which part of the phospholipid molecule influences the fluidity of a lipid bilayer? The hydrophobic fatty acid tails 10) What is keratin? A cytoplasmic intermediate filament protein 11) Which of the following peptides would you expect to interact with the active site of a protein kinase? a. Leucine-Aspartic acid-threonine-serine-alanine-leucine b. Leucine-Valine-Glycine-Arginine-Leucine c. Alanine-Proline-Proline-Proline-Tryptophan d. Glutamic acid-lysine-arginine-cysteine-methionine e. both (a) and (b) are correct 2

3 g. none of the above 12) In one sentence explain your answer to question 11. Peptide a is the only one that has serine, threonine or tyrosine, the only three amino acids that can be phosphorylated by a kinase 13) All enzymes function as biological catalysts by a. decreasing the free energy of an unfavorable reaction so it can occur spontaneously b. increasing the free energy of an unfavorable reaction so it can occur spontaneously c. creating electrochemical gradients within the active site d. reducing the activation energy necessary for the reaction to take place e. increasing the activation energy necessary for the reaction to take place f. decreasing both the free energy and the activation energy 14) Na + is commonly used to drive the coupled inward transport of nutrients in animal cells because a. a steep concentration gradient (high outside and low inside) is maintained by the Na + -glucose symport b. a steep concentration gradient (high outside and low inside) is maintained by the Na + K + pump c. a steep concentration gradient (low outside and high inside) is maintained by the Na + glucose symport d. a steep concentration gradient (low outside and high inside) is maintained by the Na + K + pump e. most animals eat processed junk food which is high in salt 15) The three protein complexes of the respiratory chain derived energy from to pump across the inner mitochondrial membrane a. phosphorylation electrons b. ATP hydrolysis. protons c. a Na + gradient K+ d. electrons. protons e. protons electrons 16) The transport of a molecule by a protein carrier differs from the transport of a molecule by a protein channel in that a. the transport by the channel exhibits saturation kinetics b. carriers always transport molecules into the cell while channels always transport molecules out the cell c. carriers always transport molecules out the cell while channels always transport molecules into the cell d. carriers only transport hydrophobic molecules while channels can transport hydrophobic and hydrophilic molecules e. both (a) and (b) are correct 3

4 17) Which of the following statements best defines the function of cholesterol? a. a carrier of molecules across the lipid bilayer b. an immediate source of ATP c. an important regulator of the activity of glycolytic enzymes d. an important regulator of membrane fluidity e. all of the above f. both (a) and (d) are correct 18) Hexokinase, the enzyme that catalyzes the first step in glycolysis, can accept either glucose or galactose as a substrate. From kinetic experiments you determine that the Km for galactose is 0.5 mm (millimolar) while the Km for glucose is 50 nm (nanomolar). Therefore a. hexokinase has higher affinity for galactose than glucose b. hexokinase has a higher affinity for glucose than galactose c. the concentration of glucose in the cell is likely to be approximately 1nM d. the Vmax is the same for both glucose and galactose e. both (b) and (d) are correct g. none of the above 19) For a voltage-gated ion channel, the probability of the channel being open is controlled by a. the concentration of the ion outside the cell b. the concentration of the ion inside the cell c. the membrane potential d. binding of a ligand e. phosphorylation 20) The primary function of the glucose carrier is a. the passive import of glucose into the cell b. the active transport of glucose into the cell c. the active transport of glucose out of the cell d. the coupled transport of glucose and protons into the cell e. the phosphorylation of glucose 4

5 SELECT FROM AMONG THE FOLLOWING LIST OF TERMS TO ANSWER QUESTIONS Terms may be used more than once or not at all. Only one term per question. (3 points each) a. ATP synthase l. acetyl CoA b. protein phosphatase m. ADP c. cytochrome c n. pyruvate d. phosphatidylcholine o. NADH dehydrogenase e. cholesterol p. stearic acid f. Na + K + ATPase pump q. voltage-gated Na + channel g. saturated fatty acid r. oxygen h. unsaturated fatty acid s. NADH i. hexokinase t. K + j. phosphofructokinase u. H + k. ATP v. cytochrome oxidase 21) E membrane lipid found only in animal cells 22) V this is the last of the respiratory enzyme complexes that accepts electrons 23) I this enzyme phosphorylates glucose 24) H membranes containing this molecule will be more fluid than membranes which do not contain this molecule 25) C the only protein of the electron transport chain that is not a proton pump 26) J the activity of this enzyme can be allosterically regulated by the first product of the citric acid cycle 27) N a three carbon acid that is the final product of glycolysis 28) L entry of this molecule into the Krebs cycle results in the production of citrate 29) A this protein can hydrolyze ATP to pump protons across the membrane 30) C this molecule serves as a mobile carrier that ferries electron from one respiratory enzyme complex to another respiratory enzyme complex 31) Q allows Na+ to enter the cell in response to a change in membrane potential 32) F responsible for the active export of Na+ from the cell 33) T the movement of this molecule into and out of the cell is largely responsible for establishing the resting membrane potential 5

6 TRUE OR FALSE. If false, provide a brief correct answer not to exceed the space provided. (3 points each) 34) true Animal cells use the Na+ gradient to take up nutrients actively 35) false the oxidation of glucose in the cytosol releases 100% of the total free energy potentially available from that glucose molecule the oxidation of glucose to pyruvate releases less than 10% of the total free energy available in that glucose molecule 36) true the cytochrome oxidase complex of the respiratory chain has a higher affinity for electrons than ubiquinone 37) true The Na+K+ ATPase pump helps maintain the osmotic balance of animal cells 38) true All intermediate filament proteins have a high content of α-helices 39) true Intermediate filaments are found in the nucleus as well as the cytoplasm 40) _false A competitive inhibitor reduces the Vmax of an enzyme a competitive inhibitor increases Km and has no effect on Vmax 41) true In a Lineweaver-Burke plot (1/S vs 1/V) the presence of a noncompetitive inhibitor affects the slope of the line 6

7 42) true Vmax is achieved when all of the enzyme in the reaction is bound to substrate 43) false All enzymes begin to exhibit a saturation effect at a substrate concentration that equals Km saturation effect is evident at very high substrate concentrations 44) true The centrosome is the major microtubule organizing center in cells 45) true Tubulin dimers have a bound molecule of GTP that is hydrolyzed to GDP after the tubulin assembles into a microtubule 46) true By creating a reaction pathway that couples an energetically favorable reaction to an energetically unfavorable reaction, enzymes cause otherwise impossible reactions (i.e., those with a + G) to occur 7

8 SHORT ANSWERS. Do not exceed the space provided. Please write neatly; if I can t read it, I won t grade it! (4 points each) 47) Dynamic instability refers to the behavior of cytoplasmic microtubules to switch back and forth between growing and shrinking. Mention two factors that determine whether the microtubule will be growing or shrinking and explain how these factors affect microtubule behavior. Dynamic instability occurs at the plus end and is determined by the concentration of αβ tubulin dimers and the rate of GTP hydrolysis. When the concentration of αβ dimers is high, they will be added to the plus end faster than the rate of GTP hydrolysis, resulting in a GTP cap that prevents depolymerization. If the concentration of αβ dimers is low, the rate of GTP hydrolysis would be faster that then rate of polymerization and GDP-subunits would depolymerize from the plus end because of their low affinity to the polymer. 48) Explain how intermediate filaments provide strength to a sheet of epithelial. Intermediate filaments span from one side of the cell to the opposite site and are indirectly connected to filaments in neighboring cells through desmosomes. This results in a continuous network of filaments that traverses through the entire cell sheet. 49) Explain how the cell utilizes a proton gradient to synthesize ATP. The electron transport chain generates a proton-motive force (consisting of a membrane potential and a proton concentration gradient) across the inner mitochondrial membrane. The protons flow back into the mitochondrial matrix through the Fo (transmembrane proton carrier) subunit of ATP synthase, which harnesses the energy from the proton gradient to make ATP from ADP and Pi 8

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