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1 UNCLASSIFIED AD NUMBER AD NEW LIMITATION CHANGE TO Approved for public release, distribution unlimited FROM Distribution authorized to U.S. Gov't. agencies and their contractors; Critical Technology; OCT Other requests shall be referred to Commanding Officer, Fort Detrick, Attn; SMUFD-AE-T, Frederick, MD AUTHORITY SMUFD, D/A ltr, 17 Feb 1972 THIS PAGE IS UNCLASSIFIED

2 wo- " -- -L TRANSLATION NO / DATE: LL9, ~ i I - DDC AVAILABILITY NOTICE - This document is subject to special export controls and each transmittal to foreign governments or foreign nationals may be made only with prior approval of Commanding Officer, Fort Detrick, ATTN: SMUFD-AE-T, - - Frederick, 4d r ;.S -;,- DEPRTMNTOF Fort.-Detrick THE ARfY ' Frederick, Maryland 2 ->=-.,=- :.

3 i I... Source: Naturwissenschaften 20:471, 1962 (Brief Orig-inal Comminicatio i Author: M. B. Williamson, L. DiLallo, H. B. Haley; Depts. of Biochemistry.nd " Surgery, Loyola University, Chicago SERUM LIPOPROTEIND INWOUND RXGERATION After rats hadubeen injured, a change in serum proteins appeared, specifieally in the globulin fraction. The change is characterized by a rise in alpha-agmbulin -with a simultaneous decrease in gama globulin LI,3]. During regenration of the wound, large amounts of fatty material appear in the regen-. eration tissue.j,6j. The deposition of lipids points to a change of metabolism in the organism. We investigated whether a eharge of fat metabolism is reflected by a change in serum lipoprotein level during the-regeneration of tewound. Adult female rats were fed a diet not including any protein fot 3 dafs. prior to and during the entire test. After three days, they receiied a circular wound, diai 4 cm on the back proximate to and at the level of the scapulae, under sodium-pentobarbital anesthesia. The 6ontrol group was treated in the same way but were not injured. Blood specimens were taken from a tail vein. Serum from 3 animals was combined for each analysis. 4I Four combined serum 'pecimens [eacl were taken from- the injured and the non-injured animals 2 day# prior to and en the thirc! and sixth day after inflicting the wound. The serum vas stained for lipoproteins with Sudan - black (as speciiied by NcDonald and!libiero; 7) and subjected to electrophoretic fractioning on cellulose-acetate strips. The latter were- then dried "o t room tenipcrature and the relative concentration of protein-bound lipid S-t]

4 %P S"gmined (0 with the densitometer. The same strips were subsequently stained wi'th azoca-rmine B in order to determine that fraction 'of the -lo- Sbuulin which is correlated to the ýfia. At the same time at which the serum stained, with Sudan black was fractioned, equal parts of the non-stained serum.v're subjected to electrophoretic P-ae.ioning on cellulose-acetate strips. These strips were tbln- stained with azoearmine B and the amount of protein in each fraction determined by a method desceribed earlier.the relative " di'stribution of serum protein among the different fractions was the same as iiliiktcv reported earlier. GL, 0] I Protein-bound.lini& in the -Sejum.of Injured Animals D a b r- - finiured Anbmals -. Control Group I - S-.:-iji - ai. Lipids- -b); Globul.in (c) Lipids -(b) Globulin (c) alpha-lbulin 2-223' C 35, : beta-globulin i ZI ) - a) Before and/or after injury; b) Total protein-bound lipids in %; " Proteini % total globulin I"-i-t-.--be-seen- -rom-tht --table that lipids correlated -to alpha- ant/or -I beta-globulin show no change of relative anount 3- and 6 days after injury. - The mass or the lipids is correlated to thu. first and second globulin fraction - 1. antl w4th both beta-globulin fractions of the serum protein. It would appear that the ratio of lipid to proteinunder goes no change after injury. The pro- -pi-- prti-ne 00 0

5 110 am tein fractions also remain unchanged during regeneration of the injured tissues. (<_ Bibliography 1) L. DiLallo, H. B. Haley, M. B. WIliamson: Clin Chem. 8 (1962) 2) M. B. Williamson, L. DiLallo, H. B. Haley:Ciin Chem. 8 (1962) 3) V. Probst, G. Schumacher, E. frlller: Medizinische 1958, vol 46 4) 0.W. Neuhaus, H. F. Balengo, A. M4. Chandler: Proc Soc. Exp. Biol. Med S7H.17:96o (1961) 5) Y, Hosoda: Keijo J. Med. 9:261 (1960) 6) H. B. Haley, M4. B. Williamson: Unpublished Findings,7) H. J. McDonald, L. P. Ribiero: An. Biochem. 1:178 (196o) 1 3I - 0I