Intracellular Vesicular Traffic Chapter 13, Alberts et al.

Transcription

1 Intracellular Vesicular Traffic Chapter 13, Alberts et al.

2 The endocytic and biosynthetic-secretory pathways

3 The intracellular compartments of the eucaryotic ell involved in the biosynthetic-secretory and endocytic pathways Vesicular de transporte

4 The intracellular compartments of the eucaryotic cell involved in the biosynthetic-secretory and endocytic pathways Endocytic pathway Biosynthetic-secretory pathway

5 Electron micrograph of clathrin-coated, COPI-coated, and COPII-coated vesicles

6 Utilization of different coats in vesicular traffic

7 Clathrin-coated pits and vesicles

8 The structure of a clathrin coat

9 The assembly and disassembly of a clathrin coat

10 The role of dynamin in pinching off clathrin-coated vesicles from the membrane

11 Model of COPII-coated vesicle formation Sar1 is a coat recruitment GTPase GEF in this case is Sec12

12 The postulated role of SNAREs in guiding vesicular traffic

13 Dissociation of SNARE pairs by NSF after a membrane fusion cycle is completed

14

15 A model for how snare proteins may concentrate in membrane fusion

16 The entry of the enveloped viruses into cells

17 The recruitment of cargo molecules into ER transport vesicles

18 Retention of incompletely assembled antibody molecules in the ER

19 Homotypic membrane fusion

20 Vesicular tubular clusters

21 A model for the retrieval of ER resident proteins

22 The Golgi apparatus

23 Light micrographs of the Golgi apparatus

24 A goblet cell of the small intestine

25 Histochemical stains demonstrating the biochemical compartmentalization of the Golgi apparatus

26 The functional compartmentalization of the Golgi apparatus

27 Two possible models explaining the organization of the Golgi apparatus and the transport of proteins from one cisterna to the next

28 Lysosomes

29 Three pathways to degradation in lysosomes

30 The structure of mannose 6-phosphate on a lysosomal enzyme

31 The transport of newly synthesized lysosomal hydrolases to lysosomes

32 The recognition of a lysosomal hydrolase

33 Phagocytosis by a macrophage Phagocytosis by a neutrophil

34 Initiation of Immune Responses Complex environment Barrier/Sensor Viruses Bacteria Fungi Parasites Allergens Self antigens Donor antigens Maternal/fetal antigens Food Protective response Infection Susceptibility/ Resistance Allergy/ Asthma Autoimmunity/Tolerance Transplant Immune System

35 Life Cycle of Dendritic Cells from Birth to Death BONE MARROW BLOOD TISSUE DC LYMPHATICS LYMPH NODE B B B B B DC T T T T T

36 Phases of DC maturation 1 DC (3) (3) DC 2 3 (5) (4) 0 24 TIME 48 (hrs) PATHOGEN DC T

37 Phagocytosis

38 Pinocytic vesicles form from coated pits in the plasma membrane The formation of clathrin-coated vesicles from plasma membrane

39 Caveolae in the plasma membrane of a fibroblast

40 A low-density lipoprotein (LDL) particle Normal and mutant LDL receptors

41 Possible fates for transmembrane receptor proteins that have been endocytosed

42 The receptor-mediated endocytosis of LDL red - transferrin R green- opioid R

43 The endocytic pathway from the plasma membrane to lysosomes

44 The sequestration of endocytosed proteins into internal membranes of multivesicular bodies

45 Transcytosis

46 Storage of plasma membrane proteins in recycling endosomes

47 Epithelial cells have two distinct early endosomal compartments but a common late endosomal compartment Apical The basolateral and the apical domains of the plasma membrane communicate with separate early endosomal compartments Basolateral

48 The constitutive and regulate secretory pathways

49 The three best-understood pathways of protein sorting in the trans golgi network

50 The formation of secretory vesicles

51 Exocytosis of secretory vesicles

52 Alternative processing pathways for the prohormone pro-opiomelanocortin

53 Electron micrographs of exocytosis in rat mast cells

54 Exocytosis as a localized response

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56 Two ways of sorting plasma membrane proteins in a polarized epithelial cell

57 Model of lipid rafts in the trans Golgi network

58 The formation of synaptic vesicles