Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University

Size: px
Start display at page:

Download "Mass Spectrometry at the Laboratory of Food Chemistry. Edwin Bakx Laboratory of Food Chemistry Wageningen University"

Transcription

1 Mass Spectrometry at the Wageningen University

2 Mass Spectrometry at the 3 UPLC/CE - ESI - Ion trap MS systems UPLC Thermo Acella with a Velos or VelosPro CE Beckman PA800 with a Thermo VelosPro 1 UPLC- ESI Ion mobility TOF MS system Waters Acquity H Class with Synapt G2Si 1 Prep HPLC Single quad MS Waters AutoPurifier system 1 MALDI-TOF/TOF MS Bruker - UltraFlextreme 1 Thermo DSQ Thermo - DSQ

3 Bruker UltraFlextreme MALDI-TOF/TOF MS Location: Axis-X Room L1-01 gfqrd Contact Person Henk Schols Daily responsible

4 Bruker UltraFlextreme MALDI-TOF/TOF MS Matrix Assisted Laser Desorption Ionization Time Of Flight Resolution (FWHM) (Reflector mode) Mass Accuracy < 2 ppm (internal calibration, Reflector mode) TOF/TOF Post Source Decay (PSD) and Collision Induced Dissociation (CID) Simple sample preparation Extreme fast measurement No separation in front

5 Bruker UltraFlextreme Principle of MALDI-TOF/TOF-MS

6 Bruker UltraFlextreme Applications "standard" Proteomics Identification of glycopeptides/proteins Identification of phosphopeptides/proteins Analysing intact proteins (up to 150 kda) Sequencing of peptides and proteins (DeNovo Sequencing) Profiling and identification of charged and neutral oligosaccharides Identification of phytonutrients (Saponins, Polyphenols etc) Identification of bacteria (with BioTyper)

7 ] Bruker UltraFlextreme Identification of oligosaccharides in complex mixtures In te n s. [a.u.] x H H H18 H H5 H H8 H H10 H _Auto_random4 0:N1 MS Raw Maltodextrine and Carboxymethyl inulin In te n s. [a.u.] Carboxymethyl inulin _Auto_random4 0:N5 MS Raw m/z m/z H = Hexose

8 Bruker UltraFlextreme Identification of oligosaccharides in complex mixtures Intens. [a.u.] MALDI-TOF/TOF-MS of CMI 4 11 (m/z = ) 1000 Z/Y 3 Z/Y 2 Z/Y Main structure B/C 1 B/C 2 B/C 3 Z/Y 3 Z/Y 2 Z/Y B/C 1 B/C 2 B/C carboxymethyl group m/z m/z

9 Bruker UltraFlextreme Identification of chemical modifications of proteins Intens. [a.u.] [M+H] + alac blg L M MTT :J22 MS, Smoothed 1000 [2M+H] + [3M+H] Native = Da [4M+H] After 48 h = Da 250 Intens. [a.u.] alac blg L M MTT :J23 MS, Smoothed Difference = 1137 Da Hexose = 162 Da Nr of hexose attached to protein m/z

10 Waters Acquity & Synapt G2 HDMS UPLC-ESI-TWIM-MS Location: Axis-X Room L1-07 Contact Person Henk Schols Daily responsible

11 Waters Acquity & Synapt G2Si HDMS Synapt G2Si HDMS Electrospray ionisation (ESI) Inlet: Direct infusion or UPLC Electron transfer dissociation (ETD) Travelling Wave Ion Mobility (TWIM) IM resolution 40 Time of Flight detection (TOF) Resolution (FWHM) Mass Accuracy < 1 ppm (Lock mass)

12 Waters Acquity & Synapt G2Si HDMS Acquity H Class Bio UPLC system Flow rate ml /min Quaternary pump system Bio compatible (titanium) Detection Photo diode array ( nm) Fluorescence Synapt G2Si HDMS with ETD

13 Traveling-Wave Ion Mobility Mass Spectrometry

14 Waters Acquity & Synapt G2Si HDMS Applications "standard" Proteomics Identification and quantification of glycopeptides /proteins Identification and quantification of phosphopeptides/ proteins Analysing intact proteins and modifications on intact proteins (up to 25 kda) Profiling, identification and quantification of charged and neutral oligosaccharides Identification of isomeric oligosaccharides Identification and quantification of phytonutrients (Saponins, Polyphenols etc)

15 Waters Acquity & Synapt G2Si HDMS Identification of (isomeric) oligosaccharides in a complex sample EXAMPLE: 3-AQ labelled methyl esterified galacturonic oligomers from pectin digest % Possible structures m/z Peak label 3 1 = 3 GalA + 1 Methyl

16 EXAMPLE : 3-AQ labelled methyl esterified galacturonic oligomers Ion mobility profile

17 Waters Acquity & Synapt G2Si HDMS Identification of the chemical modifications of proteins isoforms a-lactalbumin Glycosylated a-lactalbumin Mw = 162 = HEXOSE

18 Waters Autopurication LC-Prep Mass Directed auto purification system Location: Axis-X Room L1-07 Contact Person Henk Schols Daily responsible Mark Sanders

19 Waters AutoPurification system Waters Mass directed Auto Purification LC-Prep Preparative HPLC pump Quaternary pump (max. flow rate 150 ml/min) Injection At Column dilution Detectors Photo Diode Array (PDA) nm Single Quad MS ESI and APCI interface Full scan m/z Optional: Evaporate Light Scattering (ELS) Fraction collector Up to 448 test tubes

20 Waters AutoPurification system Waters Mass directed Auto Purification LC-Prep The configuration enables easy scale-up from: Analytical chromatography (micrograms) Semi-preparative chromatography (milligrams) Preparative chromatography (gram quantities)

21 Waters AutoPurification system Mass directed Auto Purification

22 Waters AutoPurification system Applications Mass directed purification of Primary metabolites Phytonutrients and a broad range of other secondary metabolites Oligosaccharides (Bio-active) peptides

23 CAT-AgroFood MS at the Remarks A broad range of MS Apparatus present at FCH The MALDI and the Synapt are complementary systems The Prep HPLC is unique fractionation system

Agilent 7700x ICP-MS

Agilent 7700x ICP-MS Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler

More information

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry

2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique

More information

1. Sample Introduction to MS Systems:

1. Sample Introduction to MS Systems: MS Overview: 9.10.08 1. Sample Introduction to MS Systems:...2 1.1. Chromatography Interfaces:...3 1.2. Electron impact: Used mainly in Protein MS hard ionization source...4 1.3. Electrospray Ioniztion:

More information

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF

Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF Impurity Identification using a Quadrupole - Time of Flight Mass Spectrometer QTOF PUSHER TOF DETECTOR ZSPRAY TM Ion Source SAMPLING CONE SKIMMER RF HEXAPOLE RF HEXAPOLE QUADRUPOLE IN NARROW BANDPASS MODE

More information

SCS Mass Spectrometry Laboratory

SCS Mass Spectrometry Laboratory SCS Mass Spectrometry Laboratory Contact Information Staff 31 Noyes Laboratory (8:00-5:00 M-F) 217-333-2545 http://scs.illinois.edu/massspec/ Furong Sun (frs@illinois.edu) Furong Sun Director Training

More information

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University

Methods in Mass Spectrometry. Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Methods in Mass Spectrometry Dr. Noam Tal Laboratory of Mass Spectrometry School of Chemistry, Tel Aviv University Sample Engineering Chemistry Biology Life Science Medicine Industry IDF / Police Sample

More information

Comparison of mass spectrometers performances

Comparison of mass spectrometers performances Comparison of mass spectrometers performances Instrument Mass Mass Sensitivity resolution accuracy Quadrupole 1 x 10 3 0.1 Da* 0.5-1.0 pmol DE-MALDI 2 x 10 4 20 ppm 1-10 fmol peptide 1-5 pmol protein Ion

More information

MALDI-TOF. Introduction. Schematic and Theory of MALDI

MALDI-TOF. Introduction. Schematic and Theory of MALDI MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein

More information

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016 LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry

More information

New Solvent Grade Targeted for Trace Analysis by UHPLC-MS

New Solvent Grade Targeted for Trace Analysis by UHPLC-MS New Solvent Grade Targeted for Trace Analysis by UHPLC-MS Subhra Bhattacharya, Deva H. Puranam, and Stephen C. Roemer Thermo Fisher Scientific Fisher Chemical, One Reagent Lane, Fair Lawn, NJ Material

More information

Analysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo, Nihon Waters, JAPAN

Analysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo, Nihon Waters, JAPAN Waters Alliance LC/MS System Analytical Conditions Chromatograms Mass Spectra Calibration Quantitation Analysis of Pesticides (II) Metribuzin & their metabolites in Rice Jun Yonekubo,, JAPAN Key Words

More information

Ion Source. Mass Analyzer. Detector. intensity. mass/charge

Ion Source. Mass Analyzer. Detector. intensity. mass/charge Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS

More information

Biological Mass Spectrometry. April 30, 2014

Biological Mass Spectrometry. April 30, 2014 Biological Mass Spectrometry April 30, 2014 Mass Spectrometry Has become the method of choice for precise protein and nucleic acid mass determination in a very wide mass range peptide and nucleotide sequencing

More information

Fundamentals of Soft Ionization and MS Instrumentation

Fundamentals of Soft Ionization and MS Instrumentation Fundamentals of Soft Ionization and MS Instrumentation Ana Varela Coelho varela@itqb.unl.pt Mass Spectrometry Lab Analytical Services Unit Index Mass spectrometers and its components Ionization methods:

More information

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis

Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS

More information

Mass Spectrometry. Actual Instrumentation

Mass Spectrometry. Actual Instrumentation Mass Spectrometry Actual Instrumentation August 2017 See also http://www.uni-bielefeld.de/chemie/analytik/ms f additional infmation 1. MALDI TOF MASS SPECTROMETRY ON THE ULTRAFLEX 2 2. ESI MASS SPECTROMETRY

More information

Biological Mass spectrometry in Protein Chemistry

Biological Mass spectrometry in Protein Chemistry Biological Mass spectrometry in Protein Chemistry Tuula Nyman Institute of Biotechnology tuula.nyman@helsinki.fi MASS SPECTROMETRY is an analytical technique that identifies the chemical composition of

More information

Polymer Additive Analysis by EI and APCI

Polymer Additive Analysis by EI and APCI Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable

More information

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications

Mass Spectrometry. - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications - Introduction - Ion sources & sample introduction - Mass analyzers - Basics of biomolecule MS - Applications Adapted from Mass Spectrometry in Biotechnology Gary Siuzdak,, Academic Press 1996 1 Introduction

More information

A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS.

A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. A NOVEL METHOD OF M/Z DRIFT CORRECTION FOR OA-TOF MASS SPECTROMETERS BASED ON CONSTRUCTION OF LIBRARIES OF MATRIX COMPONENTS. Martin R Green*, Keith Richardson, John Chipperfield, Nick Tomczyk, Martin

More information

[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS

[ APPLICATION NOTE ] APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS [ APPLICATI TE ] Ion Mobility-enabled Data-dependent Experiments Distinguishing Co-eluting Isomeric Metabolites Using an IMS-QTof Mass Spectrometer Jayne Kirk, 1 Russell Mortishire Smith, 1 Robert Beecher,

More information

Introduction to Proteomics 1.0

Introduction to Proteomics 1.0 Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using

More information

Ionization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s)

Ionization Methods. Neutral species Charged species. Removal/addition of electron(s) Removal/addition of proton(s) Ionization Methods Neutral species Charged species Removal/addition of electron(s) M + e - (M +. )* + 2e - electron ionization Removal/addition of proton(s) M + (Matrix)-H MH + + (Matrix) - chemical ionization

More information

Isomer Separation of Positively Labeled N-glycans by CE-ESI-MS

Isomer Separation of Positively Labeled N-glycans by CE-ESI-MS Isomer Separation of Positively Labeled N-glycans by CE-ESI-MS G.S.M. Kammeijer Center for Proteomics and Metabolomics CE IN THE BIOTECHNOLOGY & PHARMACEUTICAL INDUSTRIES 19 TH SYMPOSIUM ON THE PRACTICAL

More information

Solving practical problems. Maria Kuhtinskaja

Solving practical problems. Maria Kuhtinskaja Solving practical problems Maria Kuhtinskaja What does a mass spectrometer do? It measures mass better than any other technique. It can give information about chemical structures. What are mass measurements

More information

Characterization of an Unknown Compound Using the LTQ Orbitrap

Characterization of an Unknown Compound Using the LTQ Orbitrap Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown

More information

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur

Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses

More information

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry

Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Kai Scheffler, PhD BioPharma Support Expert,LSMS Europe The world

More information

Single Quadrupole Compact Mass Spectrometer for Liquid Sample

Single Quadrupole Compact Mass Spectrometer for Liquid Sample Single Quadrupole Compact Mass Spectrometer for Liquid Sample YL INSTRUMENT CO., LTD. YL9900 MS quickly provides essential information and improves the chemist's workflow. Mordern organic labs have become

More information

New Instruments and Services

New Instruments and Services New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo

More information

AB Sciex QStar XL. AIMS Instrumentation & Sample Report Documentation. chemistry

AB Sciex QStar XL. AIMS Instrumentation & Sample Report Documentation. chemistry Mass Spectrometry Laboratory AIMS Instrumentation & Sample Report Documentation AB Sciex QStar XL chemistry UNIVERSITY OF TORONTO AIMS Mass Spectrometry Laboratory Department of Chemistry, University of

More information

SYNAPT G2-S High Definition MS (HDMS) System

SYNAPT G2-S High Definition MS (HDMS) System SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business

More information

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010

Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Mass Spectrometry Course Árpád Somogyi Chemistry and Biochemistry MassSpectrometry Facility) University of Debrecen, April 12-23, 2010 Introduction, Ionization Methods Mass Analyzers, Ion Activation Methods

More information

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD

Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD Mass spectra of peptides and proteins - and LC analysis of proteomes Stephen Barnes, PhD 4-7117 sbarnes@uab.edu Overview A mass spectrum Electrospray MS Analysis of intact proteins Molecular weight calculations

More information

Components of a Mass Spectrometer

Components of a Mass Spectrometer Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,

More information

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222

Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Automated Sample Preparation/Concentration of Biological Samples Prior to Analysis via MALDI-TOF Mass Spectroscopy Application Note 222 Joan Stevens, Ph.D.; Luke Roenneburg; Tim Hegeman; Kevin Fawcett

More information

Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials

Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials Supporting information 1 2 3 Volume 71 (2015) Supporting information for article: 4 5 6 7 8 Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for

More information

Analysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS

Analysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS Analysis of N-Linked Glycans from Coagulation Factor IX, Recombinant and Plasma Derived, Using HILIC UPLC/FLR/QTof MS Ying Qing Yu Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F I T

More information

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,

MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19, MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents

More information

Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry

Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Michael McCullagh, 1 Kieran Neeson, 2 and Antonietta Gledhill 1 Waters Corporation,

More information

Mass spectrometry Technologies in Lipid chemistry

Mass spectrometry Technologies in Lipid chemistry Mass spectrometry Technologies in Lipid chemistry Rabah Soliymani University Of Helsinki Protein Chemistry Unit Biomedicum Helsinki Rabah.soliymani@helsinki.fi Complex_&_dynamic_mixtures (few copies to

More information

SUPPORTING INFORMATION:

SUPPORTING INFORMATION: 1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld

More information

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management CONTENT Chapter No. Title Page No. Abbreviations List of Figures List of Tables 1 Introduction 1 1.1 Practical aspects 4 1.2 Stability-indicating assay method (SIAM) 5 1.3 Regulatory aspects 6 1.4 Techniques

More information

New Instruments and Services

New Instruments and Services New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion

More information

Application Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry

Application Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry Bruker Daltonics Application Note # ET-17 / MT-99 Characterization of the N-glycosylation Pattern of Antibodies by ESI - and MALDI mass spectrometry Abstract Analysis of the N-glycosylation pattern on

More information

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT

OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (10) 86 2R QUALIFICATION OF EQUIPMENT ANNEX 7: QUALIFICATION OF MASS SPECTROMETERS Full document title and reference Document

More information

Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System

Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System Application Note Food Testing and Agriculture Mass-Based Purification of Natural Product Impurities Using an Agilent 126 Infinity II Preparative LC/MSD System Authors Florian Rieck and Jörg Hippler Agilent

More information

More structural information with MS n

More structural information with MS n PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with

More information

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance

Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance Bruker Daltonics Technical Note # TN-31 Redefining MALDI-TOF/TOF Performance The new ultraflextreme exceeds all current expectations of MALDI-TOF/TOF technology: A proprietary khz smartbeam-ii TM MALDI

More information

High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry

High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry Mark E. Hail 1, Brian Elliott 2, Kerry Nugent 3, Jeffrey L. Whitney 1, and David J. Detlefsen 1 1

More information

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev

Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems

More information

Metabolomics: quantifying the phenotype

Metabolomics: quantifying the phenotype Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems

More information

Supporting information

Supporting information Supporting information Figure legends Supplementary Table 1. Specific product ions obtained from fragmentation of lithium adducts in the positive ion mode comparing the different positional isomers of

More information

Ion fragmentation of small molecules in mass spectrometry

Ion fragmentation of small molecules in mass spectrometry Ion fragmentation of small molecules in mass spectrometry Jeevan Prasain jprasain@uab.edu 6-2612 Nomenclature: the main names and acronyms used in mass spectrometry Molecular ion: Ion formed by addition

More information

[ APPLICATION NOTE ] High Sensitivity Intact Monoclonal Antibody (mab) HRMS Quantification APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS

[ APPLICATION NOTE ] High Sensitivity Intact Monoclonal Antibody (mab) HRMS Quantification APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS KEYWORDS Yun Wang Alelyunas, Henry Shion, Mark Wrona Waters Corporation, Milford, MA, USA APPLICATION BENEFITS mab LC-MS method which enables users to achieve highly sensitive bioanalysis of intact trastuzumab

More information

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry

Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry Time-of-Flight Mass Spectrometry. Basic principles An attractive feature of the time-of-flight (TOF) mass spectrometer is its

More information

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution

Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution Quadrupole and Ion Trap Mass Analysers and an introduction to Resolution A simple definition of a Mass Spectrometer A Mass Spectrometer is an analytical instrument that can separate charged molecules according

More information

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector

Mass Spectrometry. Mass spectrometer MALDI-TOF ESI/MS/MS. Basic components. Ionization source Mass analyzer Detector Mass Spectrometry MALDI-TOF ESI/MS/MS Mass spectrometer Basic components Ionization source Mass analyzer Detector 1 Principles of Mass Spectrometry Proteins are separated by mass to charge ratio (limit

More information

Summary Chapter 8 CHAPTER 8. Summary. Page 173

Summary Chapter 8 CHAPTER 8. Summary. Page 173 CHAPTER 8 Summary Page 173 Chapter 2: Liquid Chromatography/Tandem Mass Spectrometry Method for Quantitative Estimation of PEG 400 and its Applications A rapid sensitive and selective MRM based method

More information

Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules

Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Advantages of Ion Mobility Q-TOF for Characterization of Diverse Biological Molecules Add a New Dimension to your Research Capability with Agilent s New Drift Ion Mobility Q-TOF System Overview: 6560 IM

More information

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides Mohammed Abrar Unilabs York Bioanalytical Solutions Outline Introduction Why LC-MS/MS? Limitations of

More information

Data Independent MALDI Imaging HDMS E for Visualization and Identification of Lipids Directly from a Single Tissue Section

Data Independent MALDI Imaging HDMS E for Visualization and Identification of Lipids Directly from a Single Tissue Section Data Independent MALDI Imaging HDMS E for Visualization and Identification of Lipids Directly from a Single Tissue Section Emmanuelle Claude, Mark Towers, and Kieran Neeson Waters Corporation, Manchester,

More information

Mass Spectrometry Infrastructure

Mass Spectrometry Infrastructure Mass Spectrometry Infrastructure Todd Williams, Ph.D. Director KU Mass Spectrometry and Analytical Proteomics Laboratory Mass Spectrometry Lab B025 Malott Hall Mission The Mass Spectrometry and analytical

More information

Edgar Naegele. Abstract

Edgar Naegele. Abstract Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in

More information

Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application

Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Pharmaceutical Author Cliff Woodward Agilent Technologies, Inc. 285 Centerville

More information

Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System

Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System Keywords Introduction Application Note PHA0413 High Pressure Liquid Chromatography

More information

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions

O O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism

More information

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system

Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 1290 Infinity II/6230B LC/TOF system Cannabinoid Profiling and Quantitation in Hemp Extracts using the Agilent 9 Infinity II/63B LC/TOF system Application Brief Authors Mike Adams, Karen Kaikaris, and A. Roth CWC Labs Joan Stevens, Sue D

More information

PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES. DR. A. RAMESH, Ph.D, D.Sc.,

PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES. DR. A. RAMESH, Ph.D, D.Sc., PHOTOCATALYTIC DECONTAMINATION OF CHLORANTRANILIPROLE RESIDUES IN WATER USING ZnO NANOPARTICLES DR. A. RAMESH, Ph.D, D.Sc., raamesh_a@yahoo.co.in 1 OBJECTIVES Determination of persistence and photolysis

More information

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27

An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 An Alternative Approach: Top-Down Bioanalysis of Intact Large Molecules Can this be part of the future? Lecture 8, Page 27 Top-down HRAM Bioanalysis of Native Proteins/Molecules Relative Abundance 100

More information

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA

More information

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform

Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation

More information

Small Molecule Science: Experimental designs for achieving ultra trace analysis

Small Molecule Science: Experimental designs for achieving ultra trace analysis Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small

More information

An Introduction. 1 Introduction MALDI-TOF/TOF_version100623

An Introduction. 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF/TOF mass spectrometry An Introduction 1 Introduction MALDI-TOF/TOF_version100623 MALDI-TOF(/TOF) mass spectrometry MALDI ion source Time-of of-flight (TOF) mass analyzer detector 2 Introduction

More information

MALDI Imaging Mass Spectrometry

MALDI Imaging Mass Spectrometry MALDI Imaging Mass Spectrometry Nan Kleinholz Mass Spectrometry and Proteomics Facility The Ohio State University Mass Spectrometry and Proteomics Workshop What is MALDI Imaging? MALDI: Matrix Assisted

More information

Nature Biotechnology: doi: /nbt Supplementary Figure 1

Nature Biotechnology: doi: /nbt Supplementary Figure 1 Supplementary Figure 1 The timeline of the NGAG method for extraction of N-linked glycans and glycosite-containing peptides. The timeline can be changed based on the number of samples. Supplementary Figure

More information

Reducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionkey/ms

Reducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionkey/ms Reducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionkey/ms Erin E. Chambers and Kenneth J. Fountain Waters Corporation, Milford,

More information

Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS

Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher

More information

MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine

MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Application Note: 346 MS/MS as an LC Detector for the Screening of Drugs and Their Metabolites in Race Horse Urine Gargi Choudhary and Diane Cho, Thermo Fisher Scientific, San Jose, CA Wayne Skinner and

More information

High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column

High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column Application Note BioPharma Authors James Martosella, Phu Duong, and Alex Zhu Agilent Technologies, Inc. Abstract

More information

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID

Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID Simple Method (IS-MRM) to Monitor Lysophospholipids and Phospholipids During LC-MS Method Development via In-Source CID James Little, Eastman Chemical Company, Kingsport, TN Overview Phospholipids and

More information

MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation

MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN. Dissertation MASS SPECTROMETRIC CHARACTERIZATION OF ELASTIN PEPTIDES AND THE EFFECT OF SOLAR RADIATION ON ELASTIN Dissertation zur Erlangung des akademischen Grades Doktor rerum naturalium (Dr. rer. nat.) vorgelegt

More information

Analysis of HMF by HPLC

Analysis of HMF by HPLC COST Action 927 Training School Building Skills on the Analysis of Thermal Process Contaminants in Foods 22-26 October 2007, Ankara Analysis of HMF by HPLC Vural Gökmen O O OH Background O COOH O R 2 Carbonyl

More information

Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices

Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Application ote #LCMS-2 esquire series Application of LC/Electrospray Ion Trap Mass Spectrometry for Identification and Quantification of Pesticides in Complex Matrices Introduction The simple monitoring

More information

Analytical Challenges in Veterinary Toxicology: Bromethalin

Analytical Challenges in Veterinary Toxicology: Bromethalin Analytical Challenges in Veterinary Toxicology: Bromethalin Mike Filigenzi, Birgit Puschner, Linda Aston, Robert Poppenga CAHFS-UC Davis School of Veterinary Medicine First things first: Acknowledgements

More information

UPLC-MS/MS Analysis of Azole Antifungals in Serum for Clinical Research

UPLC-MS/MS Analysis of Azole Antifungals in Serum for Clinical Research Stephen Balloch and Gareth Hammond Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity afforded by mass selective detection Wide linear measuring range Simple, inexpensive sample

More information

Dr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1

Dr. Erin E. Chambers Waters Corporation. Presented by Dr. Diego Rodriguez Cabaleiro Waters Europe Waters Corporation 1 Development of an SPE-LC/MS/MS Assay for the Simultaneous Quantification of Amyloid Beta Peptides in Cerebrospinal Fluid in Support of Alzheimer s Research Dr. Erin E. Chambers Waters Corporation Presented

More information

Bruker Daltonics. autoflex III smartbeam. The Standard in MALDI-TOF Performance MALDI-TOF/TOF. think forward

Bruker Daltonics. autoflex III smartbeam. The Standard in MALDI-TOF Performance MALDI-TOF/TOF. think forward Bruker Daltonics autoflex III smartbeam The Standard in MALDI-TOF Performance think forward MALDI-TOF/TOF Designed for a Routine High Level of Performance The autoflex III smartbeam brings the power of

More information

High Throughput Accurate Mass Screening of Monoclonal Antibodies

High Throughput Accurate Mass Screening of Monoclonal Antibodies High Throughput Accurate Mass Screening of Monoclonal Antibodies Jim Blasberg April 28, 211 sigma-aldrich.com Life Science and High Technology Center Life Science and High Technology Center2 Sigma-Aldrich,

More information

Identification of Steroids in Water by Ion Trap LC/MS/MS Application

Identification of Steroids in Water by Ion Trap LC/MS/MS Application Identification of Steroids in Water by Ion Trap LC/MS/MS Application Environmental Author Paul Zavitsanos and Christine Miller Agilent Technologies, Inc. Centerville Road Wilmington, DE 9- USA Abstract

More information

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations

Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations Advancing your Forensic Toxicology Analyses; Adopting the Latest in Mass Spectrometry Innovations For Research Use Only. Not for use in diagnostic procedures. 1 2015 AB Sciex RUO-MKT-11-1018-A For research

More information

Advances in Hybrid Mass Spectrometry

Advances in Hybrid Mass Spectrometry The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap

More information

Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF

Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Analysis of Peptides via Capillary HPLC and Fraction Collection Directly onto a MALDI Plate for Off-line Analysis by MALDI-TOF Application Note 219 Joan Stevens, PhD; Luke Roenneburg; Kevin Fawcett (Gilson,

More information

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University

Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University Mass Spectrometry and Proteomics - Lecture 1 - Matthias Trost Newcastle University matthias.trost@ncl.ac.uk Content Lectures 1-3 The basics of mass measurement Ionisation techniques Mass analysers Detectors

More information

Introduction to LC/MS/MS

Introduction to LC/MS/MS Trends in 2006 Introduction to LC/MS/MS By Crystal Holt, LC/MS Product Specialist, Varian Inc. Toxicology laboratories Increased use of LC/MS Excellent LD Cheaper (still expensive) Much more robust Solves

More information

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies.

Using Software Tools to Improve the Detection of Impurities by LC/MS. Application Note. Christine Miller Agilent Technologies. Using Software Tools to Improve the Detection of Impurities Application Note Christine Miller Introduction The analysis of raw materials and finished products for or impurities presents a challenge in

More information

Measuring Lipid Composition LC-MS/MS

Measuring Lipid Composition LC-MS/MS Project: Measuring Lipid Composition LC-MS/MS Verification of expected lipid composition in nanomedical controlled release systems by liquid chromatography tandem mass spectrometry AUTHORED BY: DATE: Sven

More information

New Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses

New Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses New Developments in LC-IMS-MS Proteomic Measurements and Informatic Analyses Erin Shammel Baker Kristin E. Burnum-Johnson, Xing Zhang, Cameron P. Casey, Yehia M. Ibrahim, Matthew E. Monroe, Tao Liu, Brendan

More information

Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids

Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids PO-CON1576E Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids HPLC 2015 PSB-MULTI-06 Yoshiyuki WATABE, Tetsuo IIDA, Daisuke NAKAYAMA, Kanya TSUJII, Saki

More information

Sample Preparation is Key

Sample Preparation is Key PLOS ONE DOI: 10.1371/journal.pone.0117232 February 6, 2015 Presented by Katie Gibbs Sample Preparation is Key Sample extraction and instrumental analysis methods are well documented in metabolomics. Understanding

More information