Ultimate Core Performance to Maximize Your Investment

Transcription

1 Thermo Scientific Accucore HPL olumns Phase Overview Ultimate ore Performance to Maximize Your Investment

2 Phase Overview Founded on state-of-the-art ore Enhanced Technology and utilizing vast experience in phase bonding and packing, Accucore HPL columns provide a unique chromatography solution to enhance laboratory workflow and efficiency. Available in a wide range of stationary phase selectivities and compatible with almost any instrument, these columns provide an excellent return on investment ontaining solid core particles, which are engineered to a diameter of 2.6 µm and a very narrow particle size distribution; Accucore HPL columns allow high speed, high resolution separation, with back pressures significantly lower than those associated with UHPL. The range of Accucore HPL columns for biomolecules are packed with 150 Å pore diameter particles and allow biomolecule separations to benefit from the superb resolution and high speed enabled by ore Enhanced Technology. Using 4 µm solid core particles, Accucore XL HPL columns allow users of conventional HPL methods to enjoy performance far beyond that of columns packed with 5 µm, 4 µm or even 3 µm fully porous particles. Phase Details olumn Phase Name Particle Type arbon Load USP ategory Accucore 18 olumns RP-MS 2.6 µm 7% µm 9% L µm 5% L7 aq 2.6 µm 9% L1 Polar Premium 2.6 µm 8% L60 Phenyl-Hexyl 2.6 µm 5% L11 PFP 2.6 µm 5% L43 Phenyl-X 2.6 µm 6% µm 5% L62 HILI 2.6 µm L3 Urea-HILI 2.6 µm Accucore olumns for Biomolecules µm 7% L µm 2% L Amide-HILI 2.6 µm Accucore XL HPL olumns 18 4 µm 7% L1 Particle Details Particle Diameter (µm) Porous Layer Thickness (µm) 8 4 µm 4% L7 Particle Size Distribution (D 90/10 ) Pore Diameter (Å) Surface Area (m 2 /g)

3 ph Stability Phases ph Range Accucore 18, , XL 18 1 to 11 Accucore Polar Premium 1.5 to 10.5 Accucore RP-MS, 8, aq, 150-4, XL 8 2 to 9 Accucore Phenyl-Hexyl, PFP, Phenyl-X, 2 to 8 30, HILI, Urea-HILI, 150-Amide-HILI Temperature Stability Maximum Temperature 70 Based on testing on Accucore 18 Flow Rate, Injection Volume and Backpressure olumns olumn ID (mm) Optimum Flow Rate (ml/min) Shipping Solvents Optimum Inj Volume (µl) Accucore HPL olumns Accucore olumns for Biomolecules Accucore XL HPL olumns Max. Backpressure Rating (bar) olumns All columns Solvent Acetonitrile/Water leaning Procedures Before starting ensure that the solvent in the column is miscible with the cleaning solvents. Using 50% of the working flow rate, flush the column with 20 column volumes of each of the solvents below in the order shown. Phase Names Accucore RP-MS, 18, 8, aq, Polar Premium, Phenyl-Hexyl, PFP, Phenyl-X, 30, , 150-4, XL 18, XL 8 HILI, Urea-HILI, 150-Amide-HILI Solvents 1. 90% water / 10% acetonitrile (for buffer removal if required) 2. 50% THF / 50% acetonitrile 3. acetonitrile 4. Re-equilibrate with mobile phase 1. 90% water / 10% acetonitrile (for buffer removal if required) 2. 50% water / 50% acetonitrile 3. Re-equilibrate with mobile phase.

4 Phase Properties and haracterization Thermo Scientific Accucore HPL columns are available in sixteen different phases to provide an unrivalled range of selectivities. Each of the bonded phases is manufactured using our advanced bonding technology and is characterized using a detailed testing regime (refer to the technical guide for full details, visit The radar plots below show the results of the characterisation and allow for quick and easy comparison of the phase selectivities. Accucore RP-MS Accucore 18 Accucore 8 HB HB HB Optimized for MS detection, excellent combination of speed and quality of separation Optimum retention for non-polar analytes Lower hydrophobicity than 18 recommended for analytes with moderate hydrophobicity Accucore aq Accucore Polar Premium Accucore Phenyl-Hexyl HB HB HB ompatible with 100% aqueous mobile phases, special selectivity for polar analytes Rugged amide embedded 18 phase that offers complementary selectivity to conventional 18 Unique selectivity for aromatic and moderately polar analytes Accucore Phenyl-X Accucore 30 HB HB Unique reversed-phase shape selectivity with high aromatic selectivity High shape selectivity for hydrophobic, long chain, structurally related isomers

5 Accucore PFP Accucore HILI Accucore Urea-HILI HILI HILI HB Alternative selectivity to 18, particularly for halogenated analytes Enhanced Retention of polar and hydrophilic analytes Unique HILI selectivity and low ion exchange activity Accucore 150-Amide-HILI Accucore Accucore HILI HB HB Designed for the separation of hydrophilic biomolecules in HILI mode. An excellent choice for glycan separations Phase designed for the separation of peptides Lower hydrophobicity for optimal retention of proteins and larger peptides Accucore XL 18 Accucore XL 8 HB HB ompatible with conventional HPL methods providing optimum retention for non-polar analytes Lower hydrophobicity than 18 recommended for analytes with moderate hydrophobicity and compatible with conventional HPL methods

6 Ordering Information Accucore HPL olumns Accucore Accucore RP-MS Accucore Accucore aq Accucore Polar Premium Accucore Phenyl-Hexyl Accucore PFP Accucore Phenyl-X Accucore Accucore HILI Accucore Urea-HILI

7 Accucore HPL olumns for Biomolecules Accucore Accucore Accucore 150-Amide-HILI Accucore nanoviper olumns Description Particle Size (µm) Length (mm) 75 μm ID Accucore Accucore Accucore 150-Amide-HILI Accucore XL HPL olumns Accucore XL Accucore XL Accucore Defender Guard artridges (4/pk) Accucore Accucore RP-MS Accucore Accucore aq Accucore Polar Premium Accucore Phenyl-Hexyl Accucore PFP Accucore Phenyl-X Accucore Accucore HILI Accucore Urea-HILI Accucore Accucore Accucore 150-Amide-HILI

8 Accucore XL Guard artridges (4/pk) Accucore XL Accucore XL UNIGUARD Direct-onnection Guard artridge Holders Description 2.1 mm ID 3.0 mm ID 4.6 mm ID UNIGUARD Drop-In Guard artridge Holder Standard Replacement Tip 850-RT 850-RT 850-RT For validation of the performance of Accucore columns or verification of the optimum selectivity for user s separations. Accucore Validation Kit Validate the reproducibility of Accucore. ontains 3 Accucore 18 HPL columns. Description Particle Size (µm) Length (mm) 2.1 mm ID Accucore Validation Kit V V V Accucore Narrow Selectivity Kit Verify optimum selectivity over a narrow range. ontains 1 each of Accucore 18, RP-MS and aq HPL columns. Description Particle Size (µm) Length (mm) 2.1 mm ID Accucore Narrow Selectivity Kit X VA X VA X VA Accucore Wide Selectivity Kit Verify selectivity over a wide range. ontains 1 each of Accucore 18, Phenyl-Hexyl and PFP HPL columns. Description Particle Size (µm) Length (mm) 2.1 mm ID Accucore Wide Selectivity Kit X VB X VB X VB Accucore Polar Selectivity Kit Verify selectivity for polar analytes. ontains 1 each of Accucore aq, PFP and HILI HPL columns. Description Particle Size (µm) Length (mm) 2.1 mm ID Accucore Polar Selectivity Kit X V X V X V 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. USA and anada France +33 (0) Germany +49 (0) or 21 United Kingdom +44 (0) Japan BR20671_E 11/12S hina India Australia (free call domestic) New Zealand (free call domestic) All Other Enquiries +44 (0) Technical Support North America Outside North America +44 (0)