cell respiration bi Biology Junction Everything you need in Biology Cellular Respiration All Materials Cmassengale

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1 Biology Junction Everything you need in Biology cell respiration bi Cellular Respiration All Materials Cmassengale C6H12O6 + 6O2 > 6CO2 + 6H20 + energy (heat and ATP) Energy 1/16

Capacity to move or change matter Forms of energy are important to life include Chemical, radiant (heat & light), mechanical, and electrical Energy can be transformed from one form to another

2 Capacity to move or change matter Forms of energy are important to life include Chemical, radiant (heat & light), mechanical, and electrical Energy can be transformed from one form to another Chemical energy is the energy contained in the chemical bonds of molecules Radiant energy travels in waves and is sometimes called electromagnetic energy. An example is visible light Photosynthesis converts light energy to chemical energy Energy that is stored is called potential energy Laws of Thermodynamics 2/16

1st law- Energy cannot be created or destroyed. Energy can be converted from one form to another. The sum of the energy before the conversion is equal to the sum of the energy after the conversion.

3 1st law- Energy cannot be created or destroyed. Energy can be converted from one form to another. The sum of the energy before the conversion is equal to the sum of the energy after the conversion. 2nd law- Some usable energy is lost during transformations. During changes from one form of energy to another, some usable energy is lost, usually as heat. The amount of usable energy therefore decreases. Adenosine triphosphate (ATP) Energy carrying molecule used by cells to fuel their cellular processes ATP is composed of an adenine base, ribose sugar, & 3 phosphate (PO4) groups The PO4 bonds are high-energy bonds that require energy to be made & release energy when broken 3/16

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ATP is made & used continuously by cells Every minute all of an organism s ATP is

to ADP ADP + Pi + energy «ATP + H2O Enzymes (ATP synthetase& ATPase) help break &

phosphorylation When the high-energy phosphate bond is broken, it releases energy, a

5 ATP is made & used continuously by cells Every minute all of an organism s ATP is recycled Phosphorylation refers to the chemical reactions that make ATP by adding Pi to ADP ADP + Pi + energy «ATP + H2O Enzymes (ATP synthetase& ATPase) help break & reform these high energy PO4 bonds in a process called substrate-level phosphorylation When the high-energy phosphate bond is broken, it releases energy, a free phosphate group, & adenosine diphosphate (ADP) 5/16

6 Enzymes in Metabolic Pathways: Biological catalysts Speeds up chemical reactions Lowers the amount of activation energy needed by weakening existing bonds in substrates Highly speci c protein molecules Have an area called the active site where substrates temporarily join Form an enzyme-substrate complex to stress bonds Enzyme usable enzyme substrate complex 6/16

folded inner membrane Folds are called cristae Space inside cristae is called the matrix & contains DNA & ribosomes

7 Energy Carriers During Respiration: NADH: A second energy carrying molecule in the mitochondria; produces 3 ATP FADH2: A third energy carrying molecule in the mitochondria; produces 2 ATP Mitochondria: Has outer smooth, outer membrane & folded inner membrane Folds are called cristae Space inside cristae is called the matrix & contains DNA & ribosomes Site of aerobic respiration Krebs cycle takes place in matrix 7/16

Electron Transport Chain takes place in cristae Cellular Respiration Overview: C6H12O6 + 6O2 > 6CO2 + 6H20 + energy (heat and ATP) Controlled release of

are released as CO2 Generates ATP (adenosine triphosphate) The energy in one glucose molecule may be used to produce 36 ATP Involves a series of 3

8 Electron Transport Chain takes place in cristae Cellular Respiration Overview: C6H12O6 + 6O2 > 6CO2 + 6H20 + energy (heat and ATP) Controlled release of energy from organic molecules (most often glucose) Glucose is oxidized (loses e-) & oxygen is reduced (gains e-) The carbon atoms of glucose (C6H12O6) are released as CO2 Generates ATP (adenosine triphosphate) The energy in one glucose molecule may be used to produce 36 ATP Involves a series of 3 reactions Glycolysis, Kreb s Cycle, & Electron Transport Chain Glycolysis: Occurs in the cytoplasm 8/16

9 Summary of the steps of Glycolysis: a. 2 ATP added to glucose (6C) to energize it. b. Glucose split to 2 PGAL (3C). (PGAL = phosphoglyceraldehyde) c. H+ and e- (e- = electron) taken from each PGAL & given to make 2 NADH. d. NADH is energy and e- carrier. e. Each PGAL rearranged into pyruvate (3C), with energy transferred to make 4 ATP (substrate phosphorylation). f. Although glycolysis makes 4 ATP, the net ATP production by this step is 2 ATP (because 2 ATP were used to start glycolysis). The 2 net ATP are available for cell use. g. If oxygen is available to the cell, the pyruvate will move into the mitochondria & aerobic respiration will begin. 9/16

from the NADH (to alcohol + CO2 in yeast or lactic acid in muscle cells).

10 Net Yield from Glycolysis 4 NADH2 2 CO2 4 ATP ( 2 used to start reaction) h. If no oxygen is available to the cell (anaerobic), the pyruvate will be fermented by addition of 2 H from the NADH (to alcohol + CO2 in yeast or lactic acid in muscle cells). This changes NADH back to NAD+ so it is available for step c above. This keeps glycolysis going! 10/16

glycolysis di uses into matrix of mitochondria & reacts with coenzyme A to for acetyl-coa

11 Alcoholic Fermentation Lactic Acid Fermentation Aerobic Respiration: Occurs in the mitochondria Includes the Krebs Cycle & the Electron Transport Chain Pyruvic acid from glycolysis di uses into matrix of mitochondria & reacts with coenzyme A to for acetyl-coa (2-carbon compound) CO2 and NADH are also produced 11/16

Kreb s Cycle: Named for biochemist Hans Krebs Metabolic pathway that indirectly requires O2 Kreb s Cycle is also known as the Citric acid Cycle Requires 2 cycles to metabolize glucose Acetyl Co-A

12 Kreb s Cycle: Named for biochemist Hans Krebs Metabolic pathway that indirectly requires O2 Kreb s Cycle is also known as the Citric acid Cycle Requires 2 cycles to metabolize glucose Acetyl Co-A (2C) enters the Kreb s Cycle & joins with Oxaloacetic Acid (4C) to make Citric Acid (6C) Citric acid is oxidized releasing CO2, free H+, & e- and forming ketoglutaric acid (5C) Free e- reduce the energy carriers NAD+ to NADH2 and FAD+ to FADH2 Ketoglutaric acid is also oxidized releasing more CO2, free H+, & e- The cycle continues oxidizing the carbon compounds formed (succinic acid, fumaric acid, malic acid, etc.) producing more CO2, NADH2, FADH2, & ATP H2O is added to supply more H+ CO2 is a waste product that di uses out of cells Oxaloacetic acid is regenerated to start the cycle again NADH2 and FADH2 produced migrate to the Electron Transport Chain (ETC) 12/16

Net Yield from Kreb s Cycle (2 turns) 6 NADH2 2 FADH2 4 CO2 2 ATP Electron Transport Chain: Found in the inner mitochondrial membrane or cristae Contains 4 protein-based complexes that work in

13 Net Yield from Kreb s Cycle (2 turns) 6 NADH2 2 FADH2 4 CO2 2 ATP Electron Transport Chain: Found in the inner mitochondrial membrane or cristae Contains 4 protein-based complexes that work in sequence moving H+ from the matrix across the inner membrane (proton pumps) A concentration gradient of H+ between the inner & outer mitochondrial membrane occurs H+ concentration gradient causes the synthesis of ATP by chemiosmosis Energized e- & H+ from the 10 NADH2 and 2 FADH2 (produced during glycolysis & Krebs cycle) are transferred to O2 to produce H2O (redox 13/16

reaction) O2 + 4e- + 4H+ 2H2O Energy Yield from Aerobic Respiration Glycolysis Kreb s Cycle Total 4 NADH2 6 NADH2 10 NADH2 x 3 = 30 ATP 0 FADH2 2 FADH2 2 FADH2 x 2 = 4 ATP 2 ATP 2 ATP 4 ATP 38 ATP

14 reaction) O2 + 4e- + 4H+ 2H2O Energy Yield from Aerobic Respiration Glycolysis Kreb s Cycle Total 4 NADH2 6 NADH2 10 NADH2 x 3 = 30 ATP 0 FADH2 2 FADH2 2 FADH2 x 2 = 4 ATP 2 ATP 2 ATP 4 ATP 38 ATP Most cells produce molecules of ATP per glucose (66% e cient) Actual number of ATP s produced by aerobic respiration varies among cells 14/16

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Cellular Respiration

Cellular Respiration C 6 H 12 O 6 + 6O 2 -----> 6CO 2 + 6H 2 0 + energy (heat and ATP) 1. Energy Capacity to move or change matter Forms of energy are important to life include Chemical, radiant (heat