Columns The FIRST and ONLY sub-2 µm core-shell columns on the market

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1 Better UHPLC Performance Starts NW.7 µm Core-Shell * Columns The IRST and NLY sub- µm core-shell columns on the market Phenomenex India Laxmi Cyber City, Ground loor, B Block, Survey no: Kondapur, Hyderabad 8, India Tel: - ax: - indiainfo@phenomenex.com Web: *ACQUITY, UPLC, and Waters are registered trademarks of Waters Corporation. Phenomenex, Inc. is not affiliated with Waters Corporation or Agilent Technologies. Agilent is a registered trademark of Agilent Technologies, Inc.

2 Kinetex.7 µm The IRST and NLY Sub- µm Core-Shell Columns on the Market Delivering breakthrough UHPLC performance, Kinetex.7 µm core-shell technology produces increased efficiencies over traditional sub- µm columns on the market, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so you can get the most out of every analysis. Efficiency Test Mix mv Kinetex.7 µm C8 N = 8,68 p/m 7 % Higher Efficiency than the traditional fully porous sub- µm column mv Waters ACQUITY BEH.7 µm C8 N = 7,8 p/m App ID 867 App ID 86 min min Conditions for both columns Columns: Kinetex.7 µm C8 ACQUITY UPLC BEH.7 µm C8 Dimensions: x. mm Mobile Phase: Acetonitrile / Water (:) Injection Volume:. µl/min low Rate:.6 ml/min Temperature: C Detection: nm (ambient) Sample:. Acetophenone. Benzene. Toluene. Naphthalene Estrogen Mix Kinetex.7 µm XB-C8 Peak Capacity = 76 9 % Higher Peak Capacity than the traditional fully porous sub- µm column Waters ACQUITY BEH.7 µm C8 Peak Capacity = App ID App ID min min Conditions for both columns Column: Kinetex.7 µm XB-C8 ACQUITY UPLC BEH.7 µm C8 Dimensions: x. mm Mobile Phase: A: 6.9 mm Ammonium ormate ph. in % Acetonitrile B: Acetonitrile Gradient: Time (min) % A % B 6 low Rate:. ml/min Temperature: Ambient Detection: nm (ambient) Instrument: Waters ACQUITY UPLC Sample:. Estriol. 7B Estradiol. Estrone ACQUITY, UPLC, and Waters are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex, Inc. is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web:

3 The Key to Better UHPLC Performance is Core-Shell Technology The secret is in the core-shell particle morphology itself. A uniform porous silica layer is grown around a spherical solid silica core. This unique combination of precise particle architecture and sub- µm particle size provides dramatic leaps in performance in two important ways:. µm Solid Core. µm Porous Core-Shell Increasing the rate of mass transfer by decreasing the effects of diffusion.7 µm Reducing losses in efficiency with nearly monodispersed sub- µm particles The result is a significant improvement in chromatographic resolution over traditional sub- µm columns, providing more information and higher quality data. PWER TIP Core-shell technology is at the forefront of UHPLC technology and is challenging system manufacturers to develop the next generation UHPLC systems capable of taking full advantage of the performance offered. The chromatography industry agrees that Kinetex columns deliver on their promise of better UHPLC performance! Download and read journal articles and technical notes on Kinetex core-shell technology for free at: Gritti, C.A. Sanchez, T. arkas, G. Guiochon, J. Chromatogr. A 7 () -. Phenomenex, Inc. All rights reserved. Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web:

4 Kinetex.7 µm Taking UHPLC to the Next Level Higher efficiencies in sub- µm core-shell particles give increased resolving power, allowing you to better separate closely eluting peaks and readily separate compounds in complex mixtures for accurate identification. Steroids Higher % 66 % 8 % Higher Resolution (between peaks and ) Kinetex.7 µm XB-C8 Resolution (between peaks and 6) 6 Higher Resolution (between peaks 7 and 8) Waters ACQUITY BEH.7 µm C8 min 6 App ID 98 Conditions for both columns Column: Kinetex.7 µm XB-C8 ACQUITY UPLC BEH.7 µm C8 Dimensions: x. mm Mobile Phase: A: MilliQ Water B: Acetonitrile Gradient: Time (min) % A % B 7 low Rate:. ml/min Temperature: Ambient Detection: nm (ambient) Backpressure: 89 bar (Kinetex) 9 bar (ACQUITY ) Instrument: Waters ACQUITY UPLC Sample:. Hydrocortisone. Cortisone. Corticosterone. Cortisone--Acetate. DHEA (Dehydroepiandrosterone) 6. 7-Hydroxyprogesterone 7. Deoxycorticosterone 8. Progesterone App ID 98 min Column Kinetex.7 µm XB-C8 x.mm ACQUITY.7 µm BEH C8 x. mm Average Peak Width Peak Capacity. sec 7.8 Resolution between Hydrocortisone and Cortisone. (66 % increase) Resolution between DHEA (Dehydroepiandrosterone) and 7-Hydroxyprogesterone. (8 % increase) Resolution between Deoxycorticosterone and Progesterone 7. ( % increase).7 sec ACQUITY, UPLC, and Waters are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex, Inc. is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web:

5 Challenging Separations are No Match for Kinetex. Barbiturates, a class of antidepressants, are often screened for in drugs of abuse testing. Two structural isomers, pentobarbital and amobarbital have been historically difficult to separate because their chemical structures differ from each other by the placement of a methyl group (See structures below). The similarity often results in co-elution of the two analyte peaks making it difficult to quantitate each compound. The Kinetex core-shell.7 µm UHPLC column successfully separated these two compounds better and faster, allowing for cost-effective, high-throughput processing of barbiturate screens. Barbiturates.6 Kinetex.7 µm XB-C8.. 8 % Higher Resolution (between peaks and ) Structural Isomers Pentobarbital Amobarbital than the traditional fully porous sub- µm column. HN NH HN NH.8.6 R s = Waters ACQUITY BEH.7 µm C8 App ID 98 min Conditions for both columns Column: Kinetex.7 µm XB-C8 ACQUITY UPLC BEH.7 µm C8 Dimensions: x. mm Mobile Phase: mm Ammonium Acetate ph 6.7/ Acetonitrile (8:) low Rate:.9 ml/min Temperature: C Detection: nm (ambient) Backpressure: 8 bar (Kinetex) 6 bar (ACQUITY ) Instrument: Waters ACQUITY UPLC Sample:. Phenobarbital. Butalbatal. Pentobarbital. Amobarbital. Secobarbital R s =.87 App ID 98 With such high efficiencies, you now have the ability to increase your productivity! By simply shortening your column length, you can drastically reduce your analysis times without losing critical resolution. Contact us at - or indiainfo@phenomenex.com min ACQUITY, UPLC, and Waters are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex, Inc. is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web:

6 Six Selectivities. Take Your Pick. Choose from a wide range of phases for greater flexibility in UHPLC method development. Kinetex.7 µm columns come in a variety of selectivities to cover a full spectrum of applications ranging from acids and bases, to isomers and extremely polar compounds. ACN H ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN H ACN ACN ACN ACN H H H Si H H H H H H H Si H H Si H Si H H H C8 XB- C8 C8 Phenyl- Hexyl PP HILIC Endcapped C8 phase Increased retention for polar basic compounds Material Characteristics Packing Material Total Particle Size (µm) Protective isobutyl side chains Increased retention of polar acidic compounds Porous Shell (µm) Solid Core (µm) Endcapped C8 phase Less hydrophobic than a C8 phase Pore Size (Å) Effective Surface Area (m /g) Effective Carbon Load % USP Classification ph Stability* Kinetex C8.7.. L. - 8.* bar Kinetex XB-C8.7.. L. - 8.* bar Kinetex C L * bar Kinetex PP L. - 8.* bar Kinetex HILIC.7.. L bar Kinetex Phenyl-Hexyl.7.. L. - 8.* bar * Columns are ph stable from.- under isocratic conditions. Columns are ph stable.-8. under gradient conditions. Phenyl - Hexyl Greater retention and separation of aromatic hydrocarbons Pentafluorophenyl phase Unique aromatic and polar selectivity Unbonded silica phase Increased retention of polar compounds Pressure Stability rdering Information SecurityGuard.7 μm Minibore Columns (mm) ULTRA Cartridges x. x. x. x. /pk XB-C8 A-98-AN B-98-AN D-98-AN -98-AN AJ-878 C8 A-7-AN B-7-AN D-7-AN -7-AN AJ-878 C8 A-99-AN B-99-AN D-99-AN -99-AN AJ-878 PP A-76-AN B-76-AN D-76-AN -76-AN AJ-8787 HILIC A-7-AN B-7-AN D-7-AN AJ-8786 Phenyl-Hexyl B--AN D--AN --AN AJ-8788 for. mm ID SecurityGuard.7 μm MidBore Columns (mm) ULTRA Cartridges x. x. x. /pk XB-C8 A-98-Y B-98-Y D-98-Y AJ-877 C8 B-7-Y D-7-Y AJ-877 C8 A-99-Y B-99-Y D-99-Y AJ-8777 PP D-76-Y AJ-878 HILIC B-7-Y AJ-8779 Phenyl-Hexyl AJ-878 for. mm ID SecurityGuard ULTRA Cartridges require holder, Part No. AJ-9. SecurityGuard Ultra Cartridge System The SecurityGuard Ultra cartridge system protects ultra-high performance columns, like Kinetex, from damaging contaminants and microparticulates. Extend Kinetex column lifetime Simple to use Pressure rated to, psi (,78 bar) its virtually all manufacturers columns. to.6 mm ID SecurityGuard Ultra Guard Cartridge Holder ea Price AJ-9 Kinetex.6 µm is also available! ind more information and dimensions online at: If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product with the Kinetex column within days for a ULL REUND. 6 Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web:

7 What Can Switching to Kinetex Really Mean to Your Lab? Ask a Customer A new analytical method was developed which is capable of analysing 6 different API residues from production surfaces. It has been shown that the.7 µm Kinetex x. mm column was capable of resolving 6 different chemical entities with a 6-minute run time. This new analytical method will be used to replace 6 older methods thereby facilitating an annualised cost saving for the site of, [$6, USD]. Andrew Charles, John P. Dunne, Louis McMahon, and Sebastian Jurek Manufacturing and Technologies, Pfizer Grange Castle, Grange Castle Business Park, Clondalkin, Dublin, Republic of Ireland.7 µm UHPLC Columns Wonder What Kinetex Will Look Like on Your Method? Let PhenoLogix, our in-house analytical services lab, show you what Kinetex core-shell technology can do for you! PhenoLogix@phenomenex.com r visit us online at: Terms and Conditions Phenomenex reserves the right to refuse sale at its discretion. ffer applies to endusers only. Subject to Phenomenex Terms & Conditions, which may be viewed at TermsAndConditions Trademarks Kinetex, Phenomenex, MidBore, and SecurityGuard are trademarks of Phenomenex. ACQUITY, UPLC, and Waters are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Agilent is a registered trademark of Agilent Technologies, Inc. Disclaimer Px Advanced Technology Solutions Pvt. Ltd. is part of Phenomenex. Phenomenex, Inc. is in no way affiliated with Waters Corporation or Agilent Technologies. Comparative separations may not be representative of all applications. Phenomenex, Inc. All rights reserved. Phenomenex India Tel: - ax: - indiainfo@phenomenex.com Web: 7

8 Australia t: f: Austria t: -9- f: -9- Belgium t: + ()-87 f: + ()-879 beinfo@phenomenex.com Canada t: (8) -68 f: () info@phenomenex.com Denmark t: 8 88 f: nordicinfo@phenomenex.com inland t: f: nordicinfo@phenomenex.com rance t: 9 f: 9 franceinfo@phenomenex.com Germany t: f: anfrage@phenomenex.com India t: - f: - indiainfo@phenomenex.com Ireland t: 7 f: eireinfo@phenomenex.com Italy t: 67 f: 67 italiainfo@phenomenex.com Luxembourg t: + ()-87 f: + ()-879 nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: -87 f: -879 nlinfo@phenomenex.com New Zealand t: f: nzinfo@phenomenex.com Norway t: 8 f: nordicinfo@phenomenex.com Puerto Rico t: (8) -HPLC f: () info@phenomenex.com Sweden t: f: nordicinfo@phenomenex.com United Kingdom t: 6-67 f: ukinfo@phenomenex.com All other countries: Corporate ffice USA t: () - f: () info@phenomenex.com The IRST and NLY sub- µm core-shell columns on the market BR889_in_ Phenomenex products are available worldwide. or the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com

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