Whole plant regulation of sulfate uptake and distribution in cabbage Koralewska, Aleksandra Dominika

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1 University of Groningen Whole plant regulation of sulfate uptake and distribution in cabbage Koralewska, Aleksandra Dominika IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record Publication date: 2008 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Koralewska, A. D. (2008). Whole plant regulation of sulfate uptake and distribution in cabbage Groningen: s.n. Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date:

2 Acknowledgements I am very glad that four years of my PhD research have resulted in this thesis. With great pleasure I would like to express my gratitude to all who have contributed to this thesis. First of all I would like to thank my supervisor Luit J. De Kok, who not only accepted me as a PhD student, but greatly supported me during this entire period. Thank you for all the hours of valuable discussion, your never ending patience and indulgence. I appreciate that you have taught me scientific writing and handle science. I am also grateful for the opportunity of participation in international conferences and symposias. I also would like to thank Bep and Freek for your excellent technical help. You had a substantial contribution to all the results described in this thesis, without your help I would not have been able to perform such extensive experiments. Bep, thank you for critical reading and language corrections of my manuscripts and thesis. I appreciate all your interest and friendship, and that you accepted to be one of my paranymphs. Freek, thank you for your precise work and planning of the experiments. I appreciate your everyday greetings and your friendly interest. Ger, thank you for your help with all the paper work at the beginning of my stay in the Netherlands and for explanation of many letters in Dutch. Thank you for your advices and your friendly assistance with various problems all the time. I would like to thank Ineke Stulen, my second copromotor, for the valuable discussions and advices, and for the correction of the thesis. I would like to thank Prof. Dr. Theo Elzenga, my promotor, for accepting me as a PhD student and the quick arrangements of the paper work. Thank you for your advices and correction of the thesis. My sincere gratitude goes to Prof. Dr. Malcolm Hawkesford and Dr. Peter Buchner from Rothamsted Research, Harpenden, U.K. Malcolm, thank you for your interest in my research and your several visits during this time. I appreciate the valuable discussions with you and your encouragement. Peter, thank you for the probes for the sulfate transporters and the training on Northern blotting, and your hospitality. 133

3 I would like to thank Dr. Stanislav Kopriva from John Innes Centre, Norwich, U.K. that I was able to stay in your lab, for teaching me measuring the APS reductase activity and for your hospitality. I can not forget to acknowledge Peter Wiersema and Cees Vermeulen, officers of the isotope laboratory, where Northern hybridization and sulfate uptake capacity measurements were performed. Thank you for your help and advice. I would like to thank also Dick Visser for designing of the figures and Alan Lewis, my student, for his 6 months work with me on my project. Thanks also to all the members of Plant Physiology and Molecular Biology for creating a nice atmosphere at work, friendly chats, advice and help. Fatma, I appreciate your friendship and that you have accepted to be one of my paranymphs. Frank and Marcel, thanks for your help and advice at the beginning of my molecular biology work. Mark, thank you for your help with the internet company at the beginning, translating letters in Dutch and being a good colleague at work. Ana, Liping, Asia, Elske, Mei, Sumithra and Muhammad, thank you all for the nice atmosphere in our office and the interesting conversations with you. Prof. Dr. Ir. Piet Kuiper and Cecil Lapre, Desiree, Roosmarijn, Geu, thank you for the pleasant discussions during lunch breaks. Jannie, thank you for your help with sending letters. Finally, but most importantly, I would like to express my sincere thanks to my family and friends. My parents, thank you for giving me a good background, your understanding and encouragement. My lovely husband, thank you for moving with me to the Netherlands, your constant support and indulgence. Thank you also for designing my cover and helping me with the computer problems all the time. My sister, thank you for being my best friend and for corrections of the summary of my thesis in Polish. My friends, Edyta, Kim, Andrzej and Marta, Asia and Jos, Marcin and Julita, thank you for all the support and entertainment you provided. 134

4 Curriculum Vitae Aleksandra Koralewska was born on April 10, 1979 in Wałbrzych, Poland. In 1998 she finished high school and obtained a Bachelor degree in Biotechnology at the University of Wroclaw in In 2002 she was awarded a Socrates/Erasmus scholarship and from December 2002 until May 2003 she was a Diplom Student in the Max Planck Institute of Molecular Plant Physiology, Golm, Germany. She passed the master exam and obtained a Master degree in September In October 2004 she started her Ph.D. study in the Department of Plant Physiology at the University of Groningen. During this time she participated in several conferences and courses, listed below. She is permitted to defend her thesis in November International meetings 6 th International Workshop on Plant Sulfur Metabolism, Kisarazu, Chiba, Japan, May 2005: Distribution of sulfur in Brassica oleracea L. as affected by sulfur nutrition (Oral presentation). International Workshop: Crosstalks between Metabolic Pathways involving Sulfur in Plants, Braunschweig, Germany, October 2005: Sulfate uptake capacity and expression of sulfate transporters in Brassica oleracea as affected by sulfur nutrition (Oral presentation). Symposium on Sulfur-Containing Defence Compounds: Pivotal players in plant stress tolerance, Heidelberg, Germany, 4-7 October 2006: Impact of the external sulfate concentration on the expression and activity of the sulfate transporters in Brassica oleracea (Poster presentation). Sulfur Workshop Potsdam 2007, MPIMP-Golm, Germany, September 2007: Sulfate transporter and APS reductase gene expression, sulfate uptake potential and APS reductase activity in curly kale (Brassica oleracea) upon sulfate deprivation and re-supply (Oral presentation).

5 Symposium of the Task Force on Sustainable Agriculture of the Agenda 21 for the Baltic Sea Region (Baltic 21), Braunschweig, Germany, April, th Workshop on Sulfur Metabolism in Plants, Warsaw, Poland, May 2008: Regulation of expression and activity of sulfate transporters in Brassica (Oral presentation). Conference on Plant Metabolism, Banff, Alberta, Canada, July 30-August 3, 2008: Whole plant regulation of sulfate uptake and distribution in cabbage (Poster presentation). Annual Meeting of Plant Biology, Lunteren, The Netherlands, April 2005, 2006, 2007 Courses The Radiation Safety Course Level 5B, January, 2005 Presentations in English Course, February-May, 2005 Dutch Language Course, September 2005-May 2006 Mass Spectrometry Course, 1-2 November, 2006