AC Griffonia Lysate Advanced
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1 AC Griffonia Lysate Advanced Innovative Neuro-Cosmetic + Anti-Aging + Instant Tightening Tomorrow s Vision Today!
2 AC GRIFFONIA LYSATE ADVANCED Technical Information: Product Code: INCI Name: Griffonia Simplicifolia Seed Extract INCI Status: Approved Suggested Use Levels: % Suggested Applications: Anti-Aging, Neuro-cosmetics, UV Protection, Tightening Solubility: Water Soluble
3 MARKET DEMANDS + CONSUMER EXPECTATIONS The Modern Consumer Statements like Minimizes Fine Lines, Rejuvenates the Skin and Increases Moisturization no longer grab consumer s attention Today, consumer s are looking for and demanding strong statements Expectations for products that make claims such as: Immediate + Significant + Visible
4 FORMULATION TOOLS + CONSUMER EXPECTATIONS Multifunctional Ingredients Formulator s best tools are ingredients that provide multiple benefits Enhances the product s marketing appeal Minimizes inventory and production time Consumer s also prefer products that provide multiple benefits
5 AC GRIFFONIA LYSATE ADVANCED Meeting Market Demand + Expectations AC Griffonia Lysate Advanced is a natural and innovative ingredient Coincides with trends for cosmetics that provide multiple and immediate benefits in neatly packaged product Immediately tightens the skin Protects against UV induced damage Increases collagen synthesis Minimizes the appearance of fine lines and wrinkles Standardized for 1.0% 5-Hydroxy Tryptophan (HTP)
6 GRIFFONIA HISTORY + INFORMATION Griffonia simplicifolia Shrub native to Africa Griffonia seeds are rich in 5-HTP ( %) 5-HTP helps protect the seeds from UV induced damage
7 AC GRIFFONIA LYSATE ADVANCED IN ACTION Skin Tightening Benefits Contains high molecular weight carbohydrates The carbohydrates create a film on top of the skin The immediately perceivable skin tightening and lifting benefits can also be attributed to these high molecular weight carbohydrates Increases Collagen Synthesis Neurotransmitters, such as GABA, have anti-aging benefits Used to relax muscles and in turn reduce the appearance of wrinkles
8 AC GRIFFONIA LYSATE ADVANCED IN ACTION Minimizes UV Induced Damage 5-HTP is an essential amino acid limited by dietary intake Unlike other precursors, such as L-tryptophan, 5-HTP is only converted to serotonin Serotonin and the Skin Initiate growth factor production Stimulate growth of dermal fibroblasts Vasodilator to improve microcirculation Converted in skin to melatonin a potent antioxidant
9 AC GRIFFONIA LYSATE ADVANCED EFFICACY DATA In-vivo Sensorial Tightening Assay Perceived Skin Tightening Results Volunteers Benchmark with 5.0% ACB Benchmark Griffonia Lysate Advanced Mean Protocol Equipment: Sensory Study Principle of measurement: Evaluated increase in tightening properties from 0-10 (0=no perceived improvement). 3 minutes after application Subjects: 12 (f) Concentration of active used: 5.0% Test area: Eye area
10 AC GRIFFONIA LYSATE ADVANCED EFFICACY DATA In-vivo Perceivable Tightening Assay Protocol Figure 2. Subject #3 Figure 3. Subject #3 Equipment: Sensory Study Principle of measurement: Evaluated increase in tightening properties from 0-10 (0=no perceived improvement). 3 minutes after application Subjects: 12 (f) Concentration of active used: 5.0% Test area: Eye area Figure 4. Subject #8 Before Figure 5. Subject #8 After
11 Type 1 C-peptide (ng/ml) 3500 AC GRIFFONIA LYSATE ADVANCED EFFICACY DATA In-vitro Collagen Synthesis Assay 3000 Protocol 2500 ELISA Assay 2000 The specimen was incubated & washed Peroxidase substrate solution was added & the mixture was incubated again Sulfuric acid was added % AC Griffonia Lysate Advanced 0.01% Sodium Ascorbate Untreated Plate was read using a microplate reader at 450nm Figure 6. AC Griffonia Lysate Advanced increased collagen synthesis by 33% compared to the untreated & 9% more than Sodium Ascorbate.
12 Apoptosis (%) Percentage AC GRIFFONIA LYSATE ADVANCED EFFICACY DATA In-vitro Inhibition of UV Cell Apoptosis Protocol Monocytes were prepared & injected with different levels of 5-HTP or N-acetylcysteine (ACC) Each set of monocytes were exposed to a different range of UV light Degree of apoptosis was measured 0 No UV, 0.0 No UV, 10 mm UV, 10 mm 5- UV 0.0 mm 5- mm 5-HTP 5-HTP HTP HTP Figure 7. Inhibition of UV Cell Apoptosis. UV 5.0 mm ACC
13 Antioxidant Capacity (µmte) AC GRIFFONIA LYSATE ADVANCED EFFICACY DATA In-vitro ORAC Assay µM Trolox 6.25µM Trolox 0.5% AC Griffonia Lysate Advanced 0.1% AC Griffonia Lysate Advanced Figure 8. Antioxidant capacity of AC Griffonia Lysate Advanced 0.05% AC Griffonia Lysate Advanced Protocol Trolox was used as the positive control Solutions were prepared at two concentrations, as a reference Florescent measurements were taken every 2 minutes for 2 hours AC Griffonia Lysate Advanced showed antioxidant activity
14 AC GRIFFONIA LYSATE ADVANCED Technical Information: Product Code: INCI Name: Griffonia Simplicifolia Seed Extract INCI Status: Approved Suggested Use Levels: % Suggested Applications: Anti-Aging, Neuro-cosmetics, UV Protection, Tightening Solubility: Water Soluble
15 AC GRIFFONIA LYSATE ADVANCED THANK YOU For more information Visit our website!
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