CLINICAL EVIDENCE Negative Pressure Wound Therapy (NPWT)

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1 CLINICAL EVIDENCE Negative Pressure Wound Therapy (NPWT) Endoform forms part of the NPWT strategy Endoform fits seamlessly with NPWT approaches. Utilize Endoform within the wound bed to help stabilize, correct, build and organize the granulation tissue during NPWT. Endoform in combination with NPWT helps to reduce the average wound closure time by 10 days compared to NPWT alone. 1 Endoform in combination with NPWT contributes to limb salvage after surgical intervention in high risk DFUs. 2 In combination with NPWT, Endoform is easy to use, remains hydrated and does not adhere to the NWPT dressing. 3.4 Patients reported no discomfort when Endoform was used with NPWT. 3,4 Current Treatment: Weekly debridement with application of CECM and NPWT : Application of Endoform : Application of NPWT over Endoform Week 6: Wound healing using Endoform combinated with NPWT 1 Endoform can be used at all phases of wound management Stabilize Correct Build Organize Hemostasis Inflammation Proliferation Remodelling Wound Closure MKT

2 CLINICAL EVIDENCE Negative Pressure Wound Therapy (NPWT) Natural Dermal Template Antimicrobial Dermal Template References 1. Vidovic, G. and P. Sykes (2016). The use of an ovine collagen extracellular matrix dressing in conjunction with negative pressure wound therapy in the management of chronic diabetic foot ulcers. Symposium on Advanced Wound Care - Fall, Los Vegas, NA. 2. Silverman, A. (2017). Use of an Ovine Collagen with an Intact Extracellular Matrix (CECM) and negative pressure wound therapy (NPWT) as part of the wound management plan following limb salvage surgical intervention in high risk diabetic foot ulcers. Symposium on Advanced Wound Care - Spring, San Diego, CA. 3. Felton, D. (2013). Case Study 6: Lower Extremity Wound, Hollister Incrorporated. 4. Felton, D. (2013). Case Study 5: Surgical Dehiscence, Hollister Incorporated. RX Only. Prior to use, be sure to read the entire Instructions for Use package insert supplied with the product. For product questions, sampling needs, or detailed clinical questions concerning our products in the US, please call HCPCS are for reference only and subject to change. Endoform is a registered trademark of Aroa Biosurgery Limited. Manufactured for: AROA BIOSURGERY INC 340 Progress Drive, Manchester, CT Endoform Dermal Template is marketed in the USA by Appulse 2018 Aroa Biosurgery Limited MKT May 2018

3 The use of an ovine collagen extracellular matrix dressing in conjunction with negative pressure wound therapy in the management of chronic diabetic foot ulcers. Gregory Vidovic, DPM, Resident Paul Sykes, DPM, Director University General Hospital Podiatric Surgery Residency, Houston, TX Introduction: The use of negative pressure wound therapy dressings (NPWT) on acute and chronic foot wounds is not only well established, it has become a modern paradigm in the treatment of difficult-totreat, pervasive wounds. The role of NPWT in promoting healing is based on a compendium of effects included induction of granulation tissue, removal of exudates, decreasing bioburden and maintaining a hydrated healing environment. 5,6 Additionally, the application of a collagen dressing over chronic wounds has shown an overall The results demonstrated in chronic diabetic foot ulceration an average time to closure of 5.5 weeks in the treatment group using the CECM dressing and 7 weeks in the control group. This shows a mean difference of 10 days in time to wound closure when CECM as added to the regimen with NPWT. There were no adverse events reported. Conclusion: In this case series, when compared to NPWT alone, the addition of Case Study 1 Diabetic foot 3 weeks status post debridement for infection of left foot Wound history: wound that began as hallux ulceration and tunneled into the medial mid-foot along the first ray Previous treatment: hydrogel, silver alginate dressing Current treatment: Case Study 2 Right diabetic foot ulcer Charcot neuroarthropathy Wound history: secondary to Charcot collapse Previous treatment: products, and off-loading boot Current treatment: decrease in wound surface in a case series of diabetic foot ulcers. 6 CECM to NPWT has shown a difference in time to wound closure in A class of several zinc-containing serine proteases including instances of long-standing diabetic foot ulcers. interstitial collagenases, gelatinases, and stromelysins collectively are known as the matrix metalloproteases (MMPs). MMP levels have been shown to be markedly elevated in chronic wounds among a plethora of other pathologic conditions. Here we present ten cases where NPWT was combined in conjunction with ovine collagen extracellular matrix dressing (CECM)* with an overall difference in time to wound closure compared to a retrospective control group in which this dual therapy was not used. Application of CECM dressing Materials and Methods: A prospective case-control study was initiated after approval from the Application of CECM prior to NPWT institutional review board. Patient selection and enrollment was non- Application of NPWT over CECM randomized and continuous until the treatment group of ten patients was filled, with cases of NPWT paired with CECM. The control group REFERENCES 1. American Diabetes Association. Standards of Medical Care in Diabetes Diabetes Care; 38 (suppl 1): S1-S2, doi: /dc15-S001 data was constructed utilizing a retrospective analysis of the last ten 2. Leaper D, Assadian O, Edmiston CE. Approach to Chronic Wound Infections. Br J Dermatol Epub Mar 15. doi: /bjd Imirzalioglu C, Sethi S, Schneider C, Hain T, Chakraborty T, Mayser P, Domann E. Distinct polymicrobal populations in a chronic foot ulcer with implications for diagnostic and anti-infective therapy. BMC Res Notes Mar 29;7:196. doi: / patients previously treated with NPWT alone. The goal of the study was to evaluate and compare the overall time to wound closure of both groups. 4. O Meara S, Al-Kurdi D, Ologun Y, Ovington LG, Martyn-St James M, Richardson R. Antibiotics and antiseptics for venous leg ulcers.cochrane Database Syst Rev Dec 23; 12:CD Epub 2013 Dec Hasan MY, Teo R, Nather A. Negative-pressure wound therapy for management of diabetic foot wounds: a review of the mechanism of action, clinical applications, and recent developments. Diabetic Foot Ankle Jul 1;6: Haycocks S, Chadwick P, Cutting KF. Collagen matrix wound dressings and the treatment of DFUs. J Wound Care Jul;22(7):369-70, * Endoform dermal template, Distributed by Hollister Incorporated. Week 6 Week 7 Caution: Federal (USA) law restricts this device for sale by or on the order of a physician or licensed healthcare professional. Refer to Instruction for Use for contraindications, warnings, precautions and possible complications. Endoform is a trademark of Aroa Biosurgery Limited Aroa Biosurgery Limited Manufactured for: AROA BIOSURGERY INC 340 Progress Drive, Manchester, CT 06042

4 Use of an Ovine Collagen with an Intact Extracellular Matrix (CECM) and negative pressure wound therapy (NPWT) as part of the wound management plan following limb salvage surgical intervention in high risk diabetic foot ulcers. Adam Silverman, DPM Silverman Podiatry P.A., Baltimore, MD Objective: Demonstrate use of an Ovine Collagen with an Intact Extracellular Matrix (CECM)* and negative pressure wound therapy (NPWT) as part of the wound management plan following limb salvage surgical intervention in high risk diabetic foot ulcers. Case Study 1: Left Hallux Amputation Patient: 65 year-old, Diabetes, neuropathy, smoker, chronic DFU on bilateral great toes with osteomyelitis post-op infection on left foot and went back to OR for debridement and partial 1st metatarsal amputation Case Study 2: Diabetic Foot Ulcer- Wet Gangrene Patient: 70 year-old female hypertension, end-stage renal disease and on hemodialysis Case Study 3: Right Toe Gangrene and Abscess Patient: 63 year-old female, admitted medical center with right 2nd toe gangrene and abscess disease, and underwent partial right 2nd ray amputation (below) Case Study 4: Non-healing surgical wound after left partial 4th and 5th ray resection Patient: 50 year-old female presented to wound care center after partial left 4th and 5th ray resections at another facility 3 weeks prior Background: Diabetes is a disease which is becoming more and more prevalent in our society. 1 As a result, more patients are developing complex lower extremity deformities which could lead to ulcerations that often progress to infection. As medical professionals, it is important that we realize the limb threatening diabetic foot ulceration or infection as early as possible so that we can provide patients with the urgent and aggressive wound care necessary for limb salvage. Patients who suffer a limb loss are more likely to suffer contralateral limb loss or even loss of life within the next few years. 2,3 Case Descriptions: These four cases involve high risk diabetic patients who were treated with surgical intervention. As a part of post-operative wound management, CECM and NPWT were utilized. CECM was applied to the wound bed, covered with a contact layer dressing,** and then a NPWT dressing was applied. Dressings were changed two to three times a week per instructions for use. Initial wound Wound management post-op: Triple antibiotic solution packing, IV antibiotics, hyperbaric oxygen therapy (HBOT) was administered while in the hospital for a total of 5 treatments Application of CECM, contact layer dressing, and NPWT. NPWT changed 3 times weekly but CECM only added weekly Start of CECM with NPWT 3.0 cm x 7.0 cm x 0.2 cm Week 3 Beefy red granulation tissue; epithelialization starting on perimeter of wound 2.1 cm x 7.5 cm x 0.1 cm Week 6 Discontinued NPWT. Continued using CECM and gentian violet and methylene blue (GVMB) polyurethane (PU) antibacterial foam*** 0.7 cm x 5.0 cm x 0.1 cm Week 16 Wound closure Initial wound Patient presented to the clinic with wet gangrene on the right foot Surgery: Incision and drainage, partial 1st and 2nd ray amputations Wound management post-op: Triple antibiotic treatment, angioplasty 3rd post-op day, HBOT 5th day post-op, NPWT 12th day post-op, went to rehab 14th day post-op Week 5 CECM can be seen in wound bed 1.3 cm x 10.5 cm x 0.2 cm Week 8 NPWT discontinued, EDT, contact layer and compression wrap 1.1 cm x 10.3 cm x 0.2 cm Week 16 Proximal 0.5 cm x 2.2 cm x 0.1 cm Distal 0.4 cm x 0.5 cm x 0.1 cm Initial wound Wound management post-op: Antiseptic packing daily and oral antibiotics 2.0 cm x 1.0 cm x 3.0 cm 5 days Post-op Granulating surgical wound with moderate drainage and macerated edges. Tendon exposed without exposed bone. The sutures are intact proximally and distally from amputation with no pain or signs of infection associated Continue to pack would with antiseptic dressing, oral antibiotics and initiated HBOT 2.4 cm x 0.8 cm x 3.5 cm Week 4 Increased granulation tissue, no tendon exposed, no pain or signs of infection, less drainage and no maceration 1.6 cm x 0.6 cm x 2.1 cm Week cm x 0.5 cm x 0.5 cm Week 10 NPWT Discontinued. CECM, contact layer dressing, and multi-layer compression wrap initiated 1.0 cm x 0.4 cm x 0.2 cm Initial wound Previous wound management: Patient arrived to wound center with a NPWT device in place and on oral antibiotics. NPWT was continued and changed 3 times per week after wound debridement 4.5 cm x 2.2 cm x 3.6 cm Application of CECM, contact layer dressing, and NPWT. NPWT changed 2 times weekly but CECM only added weekly 4.2 cm x 0.7 cm x 2.0 cm Week cm x 0.7 cm x 1.1 cm Week 2 NPWT discontinued. CECM, contact layer dressing, and multi-layer compression wrap initiated 4.2 cm x 0.5 cm x 1.5 cm Week cm x 0.3 cm x 1.0 cm Week cm x 0.3 cm x 0.5 cm Week 10 Discontinued CECM. Continued contact layer with compressin wrap 0.3 cm x 0.2 cm x 0.3 cm Conclusion: In these cases, the use of CECM and NPWT as part of the wound management plan following limb salvage surgical intervention has assisted in the task of saving these limbs. Week 1 Can see some residual CECM in wound bed 2.6 cm x 6.8 cm x 0.1 cm Start of CECM and NPWT (wound is 3 weeks old) 3.7 cm x 15.9 cm x 1.8 cm Week 24 Wound closure Week 2-3 NPWT-only initiated at week 2 - application of CECM, contact layer dressing, and NPWT initialted week 3. NPWT changed 2 times weekly but CECM only added weekly 1.6 cm x 0.6 cm x 2.6 cm Week cm x 0.1 cm x 0.1 cm Week 18 5 weeks after wound closure Week 11 Wound closure REFERENCES National Estimates and General Information on Diabetes and Prediabetes in United States, Caution: Federal (USA) law restricts this device for sale by or on the order of a physician or licensed healthcare professional. Refer to Instruction for Use for contraindications, warnings, precautions and possible complications. Endoform is a trademark of Aroa Biosurgery Limited Aroa Biosurgery Limited Manufactured for: AROA BIOSURGERY INC 340 Progress Drive, Manchester, CT 06042

5 CASE STUDY 6 Lower Extremity Wound Patient: 73-year-old female patient admitted to acute care facility for pain and wound care needs to her right lower extremity. Patient had history of peripheral vascular disease. A wound developed secondary to the swelling in her lower leg, which was caused by a blood clot. After examination and testing, results revealed a deep venous thrombosis (DVT) Wound characteristics and prior treatment: Day 1: presentation Wound measurement at onset: Proximal ulcer: 5.2cm x 5.8cm x 1.0cm (friable, slough 20%) Distal ulcer: 2.5cm x 3.2cm x 1.0cm with 3.0cm undermining at 5 o clock (tan/brown slough 50%) Wound had a large amount of serosangenious fluid with minimal odor Periwound intact, no signs and symptoms of infection noted Pain during dressing changes was reported as 8 on a scale of 1-10 Analgesics were given intravenously to allow care and dressing changes to be performed at the bedside Treatment: Applied Endoform dermal template directly to the wound bed, covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, and negative pressure wound therapy (NPWT) was initiated 72 hours after Endoform dermal template application, the NPWT dressing was removed and reapplied Wounds were assessed and findings documented upon each dressing change (3 times) Endoform applied and rehydrated Restore Contact Layer FLEX dressing applied over Endoform Pain during dressing changes was reported to be 0 on a scale of 1-10 No medication was given during dressing change Dimensions of wound at final dressing change: Proximal: 5.5cm x 4.5cm x 0.5cm Distal: 3.5cm x 2.5cm x 0.5cm with 2.5cm undermining at 5 o clock 100% granulation tissue noted One week after initial application, patient was sent home NWPT treatment initiated NWPT removed after 72 hours

6 CASE STUDY 6 Lower Extremity Wound ASE OVERVIEW Purpose: To present a case study in which a new dermal template derived from ovine extracellular matrix (ECM), covered by a flexible non-adherent lipido-colloid contact layer, is used in conjunction with negative pressure wound therapy (NPWT). Chronic wounds are known to have a disrupted or damaged ECM. Strategies which include a collagen-based advanced wound product may be beneficial. The Endoform dermal template was assessed for its ability to remain hydrated, ease of application, and patient comfort when used with NPWT foam dressings. Method: Applied the Endoform dermal template directly to the wound bed, covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, and an NPWT dressing. Wounds were assessed and findings documented. Assessment included: Endoform dermal template ability to stay hydrated Ease of application Patient comfort when used with NPWT Application of Endoform dermal template directly to the wound bed covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, yielded quite favorable results. Based on clinical observation: There was no Endoform dermal template adherence to the contact layer There was no adherence to the foam NPWT dressing Patient reported pain was 0 on a scale of % granulation tissued was noted at end of regimen Conclusion: In this case, the ability to use a collagen-based advanced wound product to assist in healing chronic wounds, which is easy to use for clinicians and provides patient comfort, yielded positive results and is very promising. Case provided by: Deborah Felton, RN, BSN, WCC; Mount Carmel East Hospital, Columbus, OH RX Only. Prior to use, be sure to read the entire Instructions for Use package insert supplied with the product. For product questions, sampling needs, or detailed clinical questions concerning our products in the US, please call HCPCS are for reference only and subject to change. Endoform is a registered trademark of Aroa Biosurgery Limited. Manufactured for: AROA BIOSURGERY INC 340 Progress Drive, Manchester, CT Endoform Dermal Template is marketed in the USA by Appulse 2018 Aroa Biosurgery Limited

7 CASE STUDY 5 Surgical Dehiscence Patient: 74-year-old female with dehisced surgical wound Wound characteristics and prior treatment: Appendectomy Wound measurement at onset: 12.2cm x 7.0cm x 6.0cm Acute care facility, inpatient at the time of wound care. Patient was seen for negative pressure wound therapy (NPWT) dressing changes. Length of inpatient status was one week. Wound bed prior to Endoform application Treatment: Applied Endoform dermal template directly to the wound bed, covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, and NPWT was initiated. Wounds were assessed and findings documented per dressing change. Wound measurement on Day 3: 11.6cm x 4.8cm x 4.2cm Wound measurement on Day 5: 11.0cm x 4.2cm x 3.8cm Endoform application No Endoform dermal template adherence to the contact layer No adherence to foam NPWT dressing Endoform dermal template remained hydrated Clinician stated Endoform dermal template was easy to apply Patient reported no pain associated with the use of Endoform dermal template Restore Contact Layer FLEX dressing placement over Endoform

8 CASE STUDY 5 Surgical Dehiscence CASE OVERVIEW Purpose: To present a case study in which a new dermal template derived from ovine extracellular matrix (ECM), covered by a flexible non-adherent lipido-colloid contact layer, is used in conjunction with negative pressure wound therapy (NPWT). Chronic wounds are known to have a disrupted or damaged ECM. Strategies which include a collagen-based advanced wound product may be beneficial. The Endoform dermal template was assessed for its ability to remain hydrated, ease of application, and patient comfort when used with NPWT foam dressings. Method: Applied the Endoform dermal template directly to the wound bed, covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, and an NPWT dressing. Wounds were assessed and findings documented at each dressing change. Assessment included: Endoform dermal template ability to stay hydrated Ease of application Patient comfort when used with NPWT Placement of an Endoform dermal template directly to the wound bed and then covered with Restore Contact Layer FLEX dressing, a flexible non-adherent lipido-colloid contact layer, yielded quite favorable results. Based on clinical observation: There was no Endoform dermal template adherence to the contact layer There was no adherence to the foam NPWT dressing Endoform dermal template remained hydrated Clinician stated Endoform dermal template was easy to apply Patient reported no pain associated with the use of Endoform dermal template Conclusion: In this case, the ability to use a collagen-based advanced wound product to assist in healing chronic wounds, which is easy to use for clinicians and provides patient comfort, yielded positive results and is very promising. Case provided by: Deborah Felton, RN, BSN, WCC; Mount Carmel East Hospital, Columbus, OH RX Only. Prior to use, be sure to read the entire Instructions for Use package insert supplied with the product. For product questions, sampling needs, or detailed clinical questions concerning our products in the US, please call HCPCS are for reference only and subject to change. Endoform is a registered trademark of Aroa Biosurgery Limited. Manufactured for: AROA BIOSURGERY INC 340 Progress Drive, Manchester, CT Endoform Dermal Template is marketed in the USA by Appulse 2018 Aroa Biosurgery Limited

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