Multi-clonal origin of macrolide-resistant Mycoplasma pneumoniae isolates. determined by multiple-locus variable-number tandem-repeat analysis
|
|
- Leon Wilson
- 5 years ago
- Views:
Transcription
1 JCM Accepts, published online ahead of print on 30 May 2012 J. Clin. Microbiol. doi: /jcm Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 Multi-clonal origin of macrolide-resistant Mycoplasma pneumoniae isolates determined by multiple-locus variable-number tandem-repeat analysis Running title: Multi-clonal origin of macrolide-resistant M. pneumoniae Yang Liu, 1 Xinye Ye, 1 Hong Zhang, 2 Xiaogang Xu, 1 Minggui Wang 1* Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai , P. R. China 1 ; Shanghai Children s Hospital, Shanghai Jiaotong University, Shanghai , P. R. China 2 *Corresponding author. Mailing address: Institute of Antibiotics, Huashan Hospital, Fudan University, 12 M. Wulumuqi Rd., Shanghai , People s Republic of China. Phone: Fax: mgwang@fudan.edu.cn. Key Words: Subtyping; Macrolide resistance; Antimicrobial resistance epidemiology 1
2 20 Abstract 21 There was a high percentage of macrolide resistance in Mycoplasma pneumoniae clinical 22 isolates in China. The genetic relatedness of macrolide-resistant M. pneunomiae strains was investigated using the multi-locus variable-number tandem-repeat assay (MLVA). Among 152 M. pneunomiae isolates, the 137 macrolide-resistant strains were clustered into 15 MLVA types indicating that the high macrolide-resistance rate in M. pneumoniae is a result of the dissemination of the multiple resistant clones. Downloaded from on November 17, 2018 by guest 2
3 27 Mycoplasma pneumoniae is one of the most common etiological agents of 28 community-acquired respiratory tract infections, and macrolides are the first-choice 29 antibiotics for treatment of M. pneumoniae infections (14). Prior to the year 2000, macrolide resistance in M. pneunomiae is rare around the world. However, several studies from China described high percentage of macrolide-resistant isolates of M. pneumoniae, ranging from 69% to 92%, obtained from both children and adults between 2003 and 2010 (1, 7, 8, 15). While it is apparent that macrolide-resistant M. pneumoniae isolates are spreading rapidly in certain parts of Asia, the epidemiological mechanism is still unknown. There was no clear association between the macrolide-resistant M. pneumoniae isolates and the P1-RFLP subtypes or PFGE subtypes in previous studies (1). Different typing methods such as conventional PCR (6), restriction fragment length polymorphism (RFLP) (3), pulsed-field gel electrophoresis (3) were developed to differentiate subtypes of M. pneumoniae. However, M. pneumoniae is a highly homogeneous organism and all these methods have a limited power of discrimination. Multi-locus variable-number tandem-repeat analysis (MLVA), which determines the number of tandem repeat sequences (TR) at different loci in a bacterial genome, is highly discriminative and has been successfully applied in typing M. pneumoniae clinical isolates, including the macrolides resistant strains, in Europe (13). In this study, MLVA was used to determine the genetic relationships of the 137 macrolide-resistant M. pneumoniae strains from Shanghai, China in a 5 year period. 46 M. pneumoniae strains. One hundred and fifty-two unique M. pneumoniae clinical 47 isolates were obtained from bronchial aspirates of children with lower respiratory infections 48 in Shanghai, China from December 2005 to July The distribution of the isolates over 3
4 49 the time of these 5 years was as follows: a total of 3 to 10 strains were collected in March, 50 June, July and August of these 5 years; 11 to 16 strains in February, October, December and 51 September; 20 to 21 strains in January, April and May. M. pneumoniae culture, PCR amplification of the P1 gene for species identification and antimicrobial susceptibility testing were carried out as described previously (7). The CLSI breakpoint (erythromycin resistance, 1µg/ml) was used to define an isolate as resistant (2). A total of 137 macrolide resistant isolates (90.1%) were identified from our collection. PCR-RFLP analysis. P1 gene PCR-RFLP typing on all 152 clinical strains of M. pneumoniae was performed as previously described (7). M. pneumoniae clinical isolates could only be classified in two types by PCR-RFLP typing method. Of 152 clinical strains, 138 strains were belonged to type I, 12 were type II and 2 could not be identified. Variable-number tandem-repeat (VNTR) locus selection and analysis. A total of 5 VNTR loci with core sequences >9 bp were selected according to the website ( see Table S1 in the supplemental material) and the corresponding PCR primers that were the same as previously described were used for MLVA typing (4). PCR reactions were carried out in a 50 μl volume containing 25 μl of 2 GC PCR buffer (TaKaRa Biotechnology, Dalian, China), 200 μm of each of the four dntps, 10 μm of each primer set, 0.5U TaKaRa La Taq polymerase (TaKaRa Biotechnology, Dalian, China), 5 μl of template DNA and 9.5 μl of water. PCR conditions 68 were as follows: initial denaturation at 95 C for 10 min, and then 40 cycles of 95 C for 1 min, C for 1 min and 72 C for 1 min, followed by a final polymerase extension step at 72 C for min. All PCR products were sequenced and copy numbers were calculated according to the 4
5 71 number of repeats for each locus. Two reference strains of M. pneumoniae M129 (ATCC ) and FH (ATCC15531) were also included as the positive control for the MLVA 73 analysis All data were input into BioNumerics version 6.5 software (Applied Maths, Belgium), then cluster analysis and minimal spanning tree (MST) analysis were performed. The 152 M. pneumoniae clinical isolates were divided into 17 MLVA types (Table 1). Of these 152 isolates, 137 macrolide resistant strains formed 15 MLVA types. There were 8 most common MLVA types in the resistant isolates, each containing more than 10 resistant strains and accounting for 84% of the total 137 isolates (Table 1). There was no evidence of resistant clonal outbreaks according to diversified MLVA patterns during the 5-years study period. The diversified pattern was also observed in isolates collected in the same time period, e.g. 11 resistant isolates from September, 2008, 15 resistant strains from April, 2009 and 21 resistant strains from May, 2009 were clustered into 7, 8 and 9 MLVA types, respectively. MST population modeling also indicated the diversity among the tested isolates, and no dominant MLVA type could not be determined (Figure 1). Diversity of M. pneumoniae strains. Hunter-Gaston index (HGI) of MLVA typing method was calculated. The formula was as follows: HGI = 1- [nj (nj-1)]/[n (N-1)], where N refers to the total number of experimental strains and nj is the number of strains belonging. Diversity indices of the 5 loci were between and (see Table S2 in the 90 supplemental material). Locus VNTR1 had higher HGI than others. The numbers of alleles of 91 the 5 loci were between 1 and 7. Loci VNTR1 had the largest number of alleles (n=7). MST 92 modeling and cluster analysis showed the diversity among the tested isolates and no dominant 5
6 93 MLVA type could not be determined (Figure 1). 94 Our earlier studies indicated that more than 90% of M. pneumoniae strains isolated in 95 Shanghai, China were resistant to macrolides (7, 8). It is known that most of the macrolide resistant M. pneunomiae strains are caused by point mutations in 23S rrna (1, 7, 8, 15). This study used MLVA as an individual identification method to type a large number of macrolide resistant M. pneunomiae isolates for determining the origin of the resistant isolates, whether from a single colony or from multiple clones. In recent years, typing schemes based on MLVA have been designed and implemented for a number of micro-organisms of public health importance (9, 10, 11, 12). Degrange et al. first applied this MLVA method in typing M. pneumoniae isolates and were able to group 265 strains (12 strains were macrolide-resistant) into 26 MLVA types (4). The discrimination potential of MLVA was shown to be much higher than the typing approach based on the differences in the P1 encoding gene. However, MLVA analysis on the limited isolates from France and Japan did not reveal any link between a particular MLVA type and macrolide resistance (4). The present study was the largest MLVA typing worldwide on the macrolide resistant isolates so far. The 137 macrolide-resistant isolates were clustered into 15 MLVA types and none of them could be determined as dominant, indicating the absence of a particular emerging macrolide-resistant clone, and the high rate of macrolide resistance in China is resulted from the dissemination of multiple resistant clones, instead of the spreading of a single colony ACKNOWLEDGEMENTS 114 This work was supported by the National Natural Science Foundation of China (Grant No. 6
7 ). We thank very much Ken B. Waites and Li Xiao for critical review of the manuscript References 1. Cao B., et al High prevalence of macrolide resistance in Mycoplasma pneumoniae isolates from adult and adolescent patients with respiratory tract infection in China. Clin. Infect. Dis. 51: Clinical and laboratory Standards Institute Methods for antimicrobial susceptibility testing for human mycoplasmas; approved guideline M43-A. Clinical and Laboratory Standards Institute, Wayne, PA, USA. 3. Cousin-Allery A., et al Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates. Epidemiol. Infect. 124: Degrange S., et al Development of multiple-locus variable-number tandem-repeat analysis for molecular typing of Mycoplasma pneumoniae. J. Clin. Microbiol. 47: Dumke R., and E. Jacobs Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae. J. Microbiol. Methods. 86: Kong F., S. Gordon, and G.L. Gilbert Rapid-cycle PCR for detection and typing 135 of Mycoplasma pneumoniae in clinical specimens. J. Clin. Microbiol. 38: Liu Y., et al Antimicrobial susceptibility of Mycoplasma pneumoniae isolates and 7
8 137 molecular analysis of macrolide-resistant strains from Shanghai, China. Antimicrob. 138 Agents Chemother. 53: Liu Y., et al Characterization of macrolide resistance in Mycoplasma pneumoniae isolated from children in Shanghai, China. Diagn. Microbiol. Infect. Dis. 67: Schouls L.M., et al Increase in genetic diversity of Haemophilus influenzae serotype b (Hib) strains after introduction of Hib vaccination in The Netherlands. J. Clin. Microbiol. 43: Schouls L.M., et al Multiple-locus variable number tandem repeat analysis of Staphylococcus aureus: comparison with pulsed-field gel electrophoresis and spa-typing. PLoS One 4: e Schwartz S., et al Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis. J. Clin. Microbiol. 47: Spuesens E.B., et al Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing. J. Microbiol. Methods. 82: Vergnaud G., and C. Pourcel Multiple locus VNTR (variable number of tandem repeat) analysis (MLVA). In E. Stackebrandt (ed.), Molecular identification, systematics and population structure of prokaryotes. Springer-Verlag, Berlin, Germany, p Waites K.B., and D.F. Talkington Mycoplasma pneumoniae and its role as a human pathogen. Clin. Microbiol. Rev. 17: Xin D.L., et al Molecular mechanisms of macrolide resistance in clinical isolates 157 of Mycoplasma pneumoniae from China. Antimicrob. Agents Chemother. 53:
9 160 Table 1. MLVA type distribution of 152 M. pneumoniae clinical isolates MLVA Macrolide Macrolide Year distribution(number of resistant strains) type a susceptible b (n) resistant c (n) total a The MLVA types of two reference strains were not included in this table. Reference strain Mp M129 belonged to MLVA type of 13 and reference strain Mp FH alone showed a separate MLVA type. 164 b Susceptible, erythromycin MIC 0.5µg/ml. 165 c Resistant, erythromycin MIC 1µg/ml. All erythromycin-resistant strains had MIC 64µg/ml in 166 present study. 9
10 167 10
11 168 Figure legend: 169 Figure 1. Minimum spanning tree of the MLVA profiles of 152 M. pneumoniae isolates. Each 170 circle denotes a particular MLVA type (MT) indicated by a number corresponding to this genotype in parentheses. The size of the circle is proportional to the number of isolates belonging to the indicated MLVA genotype. Neighboring genotypes have the same distance. Downloaded from on November 17, 2018 by guest 11
12 Figure MT4 (4) MT17 (6) MT9 (14) MT14 (13) MT6 (4) MT12 (12) MT8 (18) MT11 (21) MT1 (2) MT2 (14) MT10 (1) MT13 (10) MT15 (5) MT7 (5) MT14 (1) MT3 (20) MT16 (2) 12
Application of real-time PCR for the detection of macrolide resistance in Belgian M. pneumoniae strains
Application of real-time PCR for the detection of macrolide resistance in Belgian M. pneumoniae strains K. Loens, S. C. Masha,, K. Lagrou, H. Goossens and M. Ieven Introduction (1) One of the smallest
More informationDevelopment of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Molecular Typing of Mycoplasma pneumoniae
JOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2009, p. 914 923 Vol. 47, No. 4 0095-1137/09/$08.00 0 doi:10.1128/jcm.01935-08 Copyright 2009, American Society for Microbiology. All Rights Reserved. Development
More informationIncreasing Genetic Relatedness of Ciprofloxacin-Resistant Streptococcus pneumoniae Isolated in Canada from 1997 to 2005
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Mar. 2008, p. 1190 1194 Vol. 52, No. 3 0066-4804/08/$08.00 0 doi:10.1128/aac.01260-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Increasing
More informationEpidemiological knowledge by genotyping Chlamydia trachomatis: an overview of recent achievements. Björn Herrmann
Epidemiological knowledge by genotyping Chlamydia trachomatis: an overview of recent achievements Björn Herrmann Department of Clinical Microbiology Uppsala University Hospital Uppsala Characteristics
More informationExploring the evolution of MRSA with Whole Genome Sequencing
Exploring the evolution of MRSA with Whole Genome Sequencing PhD student: Zheng WANG Supervisor: Professor Margaret IP Department of Microbiology, CUHK Joint Graduate Seminar Department of Microbiology,
More informationNew genomic typing method MLST
New genomic typing method MLST Bon KIMURA fingerprinting PFGE DNA multilocus sequence typingmlst alleles PFGE MLST 1990 PCR 1 PCR DNA PFGE 1 PFGE RAPDrandomly amplified polymorphic DNA 3 AFLPAmplified
More informationAnalysis of phenotypic variants of the serogroup C ET-15 clone of Neisseria meningitidis by pulsed-field gel electrophoresis.
JCM Accepts, published online ahead of print on 9 May 2007 J. Clin. Microbiol. doi:10.1128/jcm.00908-07 Copyright 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationEnterovirus 71 Outbreak in P. R. China, 2008
JCM Accepts, published online ahead of print on 13 May 2009 J. Clin. Microbiol. doi:10.1128/jcm.00563-09 Copyright 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationMolecular Epidemiology of Tuberculosis. Kathy DeRiemer, PhD, MPH School of Medicine University of California, Davis
Molecular Epidemiology of Tuberculosis Kathy DeRiemer, PhD, MPH School of Medicine University of California, Davis Overview TB transmission and pathogenesis Genotyping methods Genotyping for clinical management
More informationWhole genome sequencing
Downloaded from orbit.dtu.dk on: Dec 20, 2017 Whole genome sequencing Torpdahl, Mia; Löfström, Charlotta; Møller Nielsen, Eva Published in: Publication date: 2014 Document Version Publisher's PDF, also
More informationIsolation of High Level Macrolide Resistant Bordetella pertussis Without Transition Mutation at Domain V in Iran
Jundishapur J Microbiol. 2015 July; 8(7): e18190. Published online 2015 July 25. DOI: 10.5812/jjm.8(5)2015.18190 Research Article Isolation of High Level Macrolide Resistant Bordetella pertussis Without
More informationDissemination of Macrolide-Resistant Streptococcus pneumoniae Isolates Containing Both erm(b) and mef(a) in South Korea
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 2003, p. 5787 5791 Vol. 41, No. 12 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.12.5787 5791.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved.
More informationMolecular typing insight on diversity and antimicrobial resistance of Campylobacter jejuni from Belgian chicken meat
Molecular typing insight on diversity and antimicrobial resistance of Campylobacter jejuni from Belgian chicken meat Ihab Habib Ghent University Department of Public Health and Food Safety. Contents: Molecular
More informationMacrolide-resistant Mycoplasma pneumoniae: its role in respiratory infection
Journal of Antimicrobial Chemotherapy Advance Access published November 20, 2012 J Antimicrob Chemother doi:10.1093/jac/dks457 Macrolide-resistant Mycoplasma pneumoniae: its role in respiratory infection
More informationWeekly Influenza Surveillance Report. Week 11
Weekly Influenza Surveillance Report Week 11 Report produced: 22/03/2001 Influenza activity in Ireland For the week ending the 18/03/01, week 11, influenza activity has increased. Sentinel general practices
More informationEmergence of Klebsiella pneumoniae ST258 with KPC-2 in Hong Kong. Title. Ho, PL; Tse, CWS; Lai, EL; Lo, WU; Chow, KH
Title Emergence of Klebsiella pneumoniae ST258 with KPC-2 in Hong Kong Author(s) Ho, PL; Tse, CWS; Lai, EL; Lo, WU; Chow, KH Citation International Journal Of Antimicrobial Agents, 2011, v. 37 n. 4, p.
More informationWhole genome sequencing & new strain typing methods in IPC. Lyn Gilbert ACIPC conference Hobart, November 2015
Whole genome sequencing & new strain typing methods in IPC Lyn Gilbert ACIPC conference Hobart, November 2015 Why do strain typing? Evolution, population genetics, geographic distribution 2 Why strain
More informationANALYSIS OF MYCOPLASMA GENITALIUM STRAINS ISOLATED FROM PREGNANT WOMEN AT AN ACADEMIC HOSPITAL IN PRETORIA, SOUTH AFRICA
ANALYSIS OF MYCOPLASMA GENITALIUM STRAINS ISOLATED FROM PREGNANT WOMEN AT AN ACADEMIC HOSPITAL IN PRETORIA, SOUTH AFRICA Mafunise M 1, Le Roux MC 1, de Villiers BE 1, Ditsele RMM 1,2 1 Department of Microbiological
More informationMultiple-Locus Variable-Number Tandem-Repeat Analysis of. Acinetobacter baumannii in China: comparison with PFGE typing.
JCM Accepts, published online ahead of print on 23 January 2013 J. Clin. Microbiol. doi:10.1128/jcm.03108-12 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 2 3 4 Multiple-Locus
More informationSurveillance of Enterococci in Belgium. M. Ieven, K. Loens, B. Jans and H. Goossens
Surveillance of Enterococci in Belgium M. Ieven, K. Loens, B. Jans and H. Goossens Surveillance of Enterococci in Belgium Overview Introduction and epidemiological surveillance Results of isolates received
More informationUpdate on resistance and epidemiology of CAP pathogens in Asia. Cao Bin, MD
Update on resistance and epidemiology of CAP pathogens in Asia Cao Bin, MD Dept Infectious Diseases and Clinical Microbiology Beijing Chaoyang Hospital, Capital Medical University Outlines Resistance trends
More informationFirst detection of the mcr-1 gene in Escherichia coli isolated from livestock between 2013
AAC Accepted Manuscript Posted Online 29 August 2016 Antimicrob. Agents Chemother. doi:10.1128/aac.01472-16 Copyright 2016, American Society for Microbiology. All Rights Reserved. 1 2 First detection of
More information1) 1) 1) 2) 2) (ICU) Key words: (ICU), ( ) 1 30 TEL: FAX: Vol. 17 No
2007 277 1) 1) 1) 2) 2) 3) 4) 5) 6) 1) 2) 3) 4) 5) 6) 18 10 17 19 8 20 16 7 1 (ICU) 20 2x 1.6 2.2 2SD 14 1 4 1 10 7 3 2 9 7.6; 95 2.37 24.62 6 4 5 1 DNA SpeI 9 2 A 7 B 2 A 3 B 1 ICU ICU 7 20 10 5 Key words:
More informationFilipa Matos, Mónica V. Cunha*, Ana Canto, Teresa Albuquerque, Alice Amado, and. INRB, I.P./LNIV- Laboratório Nacional de Investigação Veterinária
JCM Accepts, published online ahead of print on 15 September 2010 J. Clin. Microbiol. doi:10.1128/jcm.01762-10 Copyright 2010, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationTable S1. Primers and PCR protocols for mutation screening of MN1, NF2, KREMEN1 and ZNRF3.
Table S1. Primers and PCR protocols for mutation screening of MN1, NF2, KREMEN1 and ZNRF3. MN1 (Accession No. NM_002430) MN1-1514F 5 -GGCTGTCATGCCCTATTGAT Exon 1 MN1-1882R 5 -CTGGTGGGGATGATGACTTC Exon
More informationIsolation and identification of Mycoplasma gallisepticum in chickensbn from industrial farms in Kerman province
Available online at http://www.ijabbr.com International journal of Advanced Biological and Biomedical Research Volume 2, Issue 1, 2014: 100-104 Isolation and identification of Mycoplasma gallisepticum
More informationPertussis: An Emerging Infection. Holly K. Ehrke. Ferris State University
Running head: PERTUSSIS AN EMERGING INFECTION 1 Pertussis: An Emerging Infection Holly K. Ehrke Ferris State University PERTUSSIS AN EMERGING INFECTION 2 Abstract Pertussis is a highly contagious disease
More informationThe Bacteriology of Bronchiectasis in Australian Indigenous children
The Bacteriology of Bronchiectasis in Australian Indigenous children Kim Hare, Amanda Leach, Peter Morris, Heidi Smith-Vaughan, Anne Chang Presentation outline What is bronchiectasis? Our research at Menzies
More informationMolecular epidemiology and drug resistance mechanism of Salmonella species especially in S. Typhi strains isolated in Bangladesh
Molecular epidemiology and drug resistance mechanism of Salmonella species especially in S. Typhi strains isolated in Bangladesh Dr. Kaisar Ali Talukder Senior Scientist Icddr,b This presentation will
More informationMRC-Holland MLPA. Description version 08; 30 March 2015
SALSA MLPA probemix P351-C1 / P352-D1 PKD1-PKD2 P351-C1 lot C1-0914: as compared to the previous version B2 lot B2-0511 one target probe has been removed and three reference probes have been replaced.
More informationa) SSR with core motif > 2 and repeats number >3. b) MNR with repeats number>5.
1 2 APPENDIX Legends to figures 3 4 5 Figure A1: Distribution of perfect SSR along chromosome 1 of V. cholerae (El-Tor N191). a) SSR with core motif > 2 and repeats number >3. b) MNR with repeats number>5.
More information- Mycoplasma pneumoniae (MP): important respiratory pathogen in children that cause many upper and lower respiratory tract diseases, including
KHOA DICH VU HO HAP - Mycoplasma pneumoniae (MP): important respiratory pathogen in children that cause many upper and lower respiratory tract diseases, including wheezing, coryza, bronchopneumonia. -
More informationin the Gastrointestinal and Reproductive Tracts of Quarter Horse Mares
Influence of Probiotics on Microflora in the Gastrointestinal and Reproductive Tracts of Quarter Horse Mares Katie Barnhart Research Advisors: Dr. Kimberly Cole and Dr. John Mark Reddish Department of
More informationS. Wesley Long, MD, PhD
Basic Molecular Microbiology: A Practical Case-Based Approach S. Wesley Long, MD, PhD Center for Molecular and Translational Human Infectious Diseases Research Houston Methodist Research Institute September
More informationRetrospective and Prospective Verification of the Cepheid Xpert Flu Assay
JCM Accepts, published online ahead of print on 20 July 2011 J. Clin. Microbiol. doi:10.1128/jcm.01162-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationMethicillin-Resistant Staphylococcus aureus (MRSA) S urveillance Report 2008 Background Methods
Methicillin-Resistant Staphylococcus aureus (MRSA) Surveillance Report 2008 Oregon Active Bacterial Core Surveillance (ABCs) Office of Disease Prevention & Epidemiology Oregon Department of Human Services
More informationSimultaneous and Rapid Detection of Causative Pathogens in Community-acquired Pneumonia by Real-time PCR (1167)
From the Japanese Association of Medical Sciences The Japanese Association for Infectious Diseases Simultaneous and Rapid Detection of Causative Pathogens in Community-acquired Pneumonia by Real-time PCR
More information#Corresponding author: Pathology Department, Singapore General Hospital, 20 College. Road, Academia, Level 7, Diagnostics Tower, , Singapore
AAC Accepts, published online ahead of print on 21 October 2013 Antimicrob. Agents Chemother. doi:10.1128/aac.01754-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 Title: Escherichia
More informationACCEPTED. Comparison of disk diffusion and agar dilution methods for erythromycin and
AAC Accepts, published online ahead of print on January 00 Antimicrob. Agents Chemother. doi:./aac.000-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationStreptococcus pneumoniae 356 moxifloxacin (MFLX), garenoxacin (GRNX) sitafloxacin
2009 21 1) 1, 2) 1, 2) 1) 1) 2) 1) 1) 1) 1) 1) 1, 2) 1) 2) 20 2 29 21 1 14 Streptococcus pneumoniae 356 moxifloxacin (MFLX), garenoxacin (GRNX) sitafloxacin (STFX), DX619 S. pneumoniae S. pneumoniae 60
More informationChapter 2.1. Meticillin-resistant Staphylococcus aureus epidemiology and transmission in a Dutch hospital
Meticillin-resistant Staphylococcus aureus epidemiology and transmission in a Dutch hospital MML van Rijen 1, T Bosch 2, MEOC Heck 2, JAJW Kluytmans 1,3 1 Laboratory for Microbiology and Infection Control,
More informationRequest for Report for Projects Awarded in 2013 and 2014 by. Mississippi Center for Food Safety and Post-Harvest Technology
Request for Report for Projects Awarded in 2013 and 2014 by Mississippi Center for Food Safety and Post-Harvest Technology Title: Quantification of high-risk and low-risk Listeria monocytogenes serotypes
More informationResistance to linezolid in enterococci and staphylococci referred to the national reference laboratory
Resistance to linezolid in enterococci and staphylococci referred to the national reference laboratory Dr Danièle Meunier, AMRHAI BSAC - Antimicrobial Susceptibility Testing User Days Oxazolidinone Linezolid
More informationConstruction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation
Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation J. Du 1, Z.H. Tao 2, J. Li 2, Y.K. Liu 3 and L. Gan 2 1 Department of Chemistry,
More informationSALSA MLPA KIT P050-B2 CAH
SALSA MLPA KIT P050-B2 CAH Lot 0510, 0909, 0408: Compared to lot 0107, extra control fragments have been added at 88, 96, 100 and 105 nt. The 274 nt probe gives a higher signal in lot 0510 compared to
More informationRespiratory Pathogen Panel TEM-PCR Test Code:
Respiratory Pathogen Panel TEM-PCR Test Code: 220000 Tests in this Panel Enterovirus group Human bocavirus Human coronavirus (4 types) Human metapneumovirus Influenza A - Human influenza Influenza A -
More informationS. Pereyre,* A. Charron, H. Renaudin, C. Bébéar, and C. M. Bébéar
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 2007, p. 3534 3539 Vol. 45, No. 11 0095-1137/07/$08.00 0 doi:10.1128/jcm.01345-07 Copyright 2007, American Society for Microbiology. All Rights Reserved. First Report
More informationXMRV among Prostate Cancer Patients from the Southern United States and Analysis of Possible Correlates of Infection
XMRV among Prostate Cancer Patients from the Southern United States and Analysis of Possible Correlates of Infection Bryan Danielson Baylor College of Medicine Department of Molecular Virology and Microbiology
More informationSensitive and specific Modified Hodge Test for KPC and metallo-beta-lactamase
JCM Accepts, published online ahead of print on 19 October 2011 J. Clin. Microbiol. doi:10.1128/jcm.05602-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All
More informationIn vitro and Intracellular Activities of Peptide Deformylase. Inhibitor GSK against Legionella pneumophila Isolates
AAC Accepts, published online ahead of print on 27 October 2014 Antimicrob. Agents Chemother. doi:10.1128/aac.04006-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 2 In vitro
More informationAppendix A: Disease-Specific Chapters
Ministry of Health and Long-Term Care Infectious Diseases Protocol Appendix A: Disease-Specific Chapters Chapter: Haemophilus influenzae disease, all types, invasive Effective: May 2018 Haemophilus influenzae,
More informationThesis for doctoral degree (Ph.D.) 2007 Molecular epidemiology of pneumococcal carriage and invasive disease Karin Sjöström.
Thesis for doctoral degree (Ph.D.) 2007 Thesis for doctoral degree (Ph.D.) 2007 Molecular epidemiology of pneumococcal carriage and invasive disease Karin Sjöström Molecular Epidemiology of Pneumococcal
More informationDiphtheria infections caused by the different toxigenic biotypes of Corynebacterium diphtheriae
JCM Accepts, published online ahead of print on 5 December 00 J. Clin. Microbiol. doi:0.8/jcm.054-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
More informationEpidemiology and control of Mycoplasma infections in swine
Epidemiology and control of Mycoplasma infections in swine Maria Pieters, DVM, PhD Maria Pieters DVM, PhD Veterinary Population Medicine department and Veterinary Diagnostic Laboratory Agenda Control Knowledge
More informationStudy on Serotype and Apx Toxins Type of Actinobacillus pleuropneumoniae Strains Isolated from Shandong Province
2005,38(11):2349-2354 Scientia Agricultura Sinica Apx 271018 : 85 APP PCR APX 20 5 APP 7 6 5 5 3 4 4 3 8 2 7 5 APP PCR APP 4 (Apx) 3 4 8 APP Apx 5 Apx 7 20 APP APP 99.5 ~100 APP Apx 5 APP PCR : Study on
More informationCharacterization of community and hospital Staphylococcus aureus isolates in Southampton, UK
Journal of Medical Microbiology (2010), 59, 1084 1088 DOI 10.1099/jmm.0.018986-0 Characterization of community and hospital Staphylococcus aureus isolates in Southampton, UK S. M. Green, 1 P. Marsh, 1
More information* these authors contributed equally to the preparation of this report
AAC Accepts, published online ahead of print on June 00 Antimicrob. Agents Chemother. doi:0./aac.000-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationKnow for sure! Your Power for Health. PapilloCheck and PapilloCheck high-risk HPV-Genotyping: The Clear Edge in Early Detection of Cervical Cancer.
Your Power for Health Laboratory Information hr-hpv DNA-Chip Know for sure! PapilloCheck and PapilloCheck high-risk HPV-Genotyping: The Clear Edge in Early Detection of Cervical Cancer. PapilloCheck and
More informationEPIDEMIOLOGY OF MORAXELLA CATARRHALIS INFECTIONS AMONG THAI PATIENTS TREATED AT SIRIRAJ HOSPITAL, THAILAND DURING
EPIDEMIOLOGY OF MORAXELLA CATARRHALIS INFECTIONS AMONG THAI PATIENTS TREATED AT SIRIRAJ HOSPITAL, THAILAND DURING 2012-2015 Chanwit Tribuddharat and Somporn Srifuengfung Department of Microbiology, Faculty
More informationOUT-TB Web. Ontario Universal Typing of Tuberculosis: Surveillance and Communication System
OUT-TB Web Ontario Universal Typing of Tuberculosis: Surveillance and Communication System Dr. Frances Jamieson, Ontario Public Health Laboratories November 30 th, 2009 Tuberculosis : A Global Problem
More informationQian Gao Fudan University
Qian Gao Fudan University Outline Background & Objectives Genotyping methods Establish the epidemiological field sites Preliminary results of Molecular epidemiology of TB in China Molecular epidemiology
More informationST11 KPC-2 Klebsiella pneumoniae detected in Taiwan
AAC Accepts, published online ahead of print on 30 January 2012 Antimicrob. Agents Chemother. doi:10.1128/aac.05576-11 Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 3 4 5
More informationEvolution of influenza
Evolution of influenza Today: 1. Global health impact of flu - why should we care? 2. - what are the components of the virus and how do they change? 3. Where does influenza come from? - are there animal
More informationTitle: Chest wall abscess due to Mycobacterium bovis BCG after intravesical BCG therapy
JCM Accepts, published online ahead of print on 23 November 2011 J. Clin. Microbiol. doi:10.1128/jcm.05888-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All
More informationÖrebro University Hospital
Örebro University Hospital Department of Laboratory Medicine Susanne Jacobsson Date: 2015-02-18 Page 1 (8) Neisseria meningitidis 2014 Annual report concerning serogroup, genosubtype and antibiotic susceptibility
More informationRésistance bactérienne au cours des Infections Sexuellement Transmissibles Cécile Bébéar
Résistance bactérienne au cours des Infections Sexuellement Transmissibles Cécile Bébéar French Na*onal Center for bacterial STIs Bordeaux University hospital, Bordeaux, France University of Bordeaux,
More informationRapid identification and resistance assessment: The future is mass spectrometry
Rapid identification and resistance assessment: The future is mass spectrometry Dr Sanmarié Schlebusch Director of Microbiology Mater Pathology Brisbane Outline Introduction Plug and play Pre-prep and
More informationLikelihood that an unsubtypeable Influenza A result. in the Luminex xtag Respiratory Virus Panel. is indicative of novel A/H1N1 (swine-like) influenza
JCM Accepts, published online ahead of print on 3 June 2009 J. Clin. Microbiol. doi:10.1128/jcm.01027-09 Copyright 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationDetection and characterization of Mycoplasma pneumoniae during an outbreak of respiratory illness at a university
JCM Accepts, published online ahead of print on 26 December 2013 J. Clin. Microbiol. doi:10.1128/jcm.02810-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 2 3 4 5 6 7 8 9 10
More informationChain of Infection Agent Mode of transmission Contact (direct, indirect, droplet spread) Airborne Common-vehicle spread Host
Goals Microbiology of Healthcare-associated Infections William A. Rutala, Ph.D., M.P.H. Director, Statewide Program for Infection Control and Epidemiology and Research Professor of Medicine, University
More informationHepatitis C Virus Genotype 2 may not be detected by the Cobas AmpliPrep/Cobas. TaqMan HCV Test, version 1.0
JCM Accepts, published online ahead of print on 25 September 2013 J. Clin. Microbiol. doi:10.1128/jcm.02102-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 2 Hepatitis C Virus
More informationOptimal Combination of VNTR Typing for Discrimination of Isolated Mycobacterium tuberculosis in Korea
ORIGINAL ARTICLE http://dx.doi.org/10.4046/trd.2014.76.2.59 ISSN: 1738-3536(Print)/2005-6184(Online) Tuberc Respir Dis 2014;76:59-65 Optimal Combination of VNTR Typing for Discrimination of Isolated Mycobacterium
More informationAvian Influenza Virus H7N9. Dr. Di Liu Network Information Center Institute of Microbiology Chinese Academy of Sciences
Avian Influenza Virus H7N9 Dr. Di Liu Network Information Center Institute of Microbiology Chinese Academy of Sciences Avian Influenza Virus RNA virus, Orthomyxoviruses Influenza A virus Eight Gene segments
More informationEmerging infectious disease: trends in the literature on SARS and H7N9 influenza
Scientometrics (2015) 105:485 495 DOI 10.1007/s11192-015-1681-8 Emerging infectious disease: trends in the literature on SARS and H7N9 influenza Deqiao Tian 1 Tao Zheng 1 Received: 19 July 2015 / Published
More informationA first molecular characterization of Listeria monocytogenes isolates circulating in humans from 2009 to 2014 in the Italian Veneto region
Short communication A first molecular characterization of Listeria monocytogenes isolates circulating in humans from 2009 to 2014 in the Italian Veneto region Cristiano Salata 1,2, Paola Lisotto 1, Caterina
More informationBe sure! Your Power for Health. PelvoCheck CT/NG Your test kit for Chlamydia trachomatis screening and Neisseria gonorrhoeae infections
Your Power for Health Laboratory Information CT/NG DNA-Chip Be sure! Your test kit for Chlamydia trachomatis screening and Neisseria gonorrhoeae infections is part of the ocheck product line from Greiner
More informationRecommended laboratory tests to identify influenza A/H5 virus in specimens from patients with an influenza-like illness
World Health Organization Recommended laboratory tests to identify influenza A/H5 virus in specimens from patients with an influenza-like illness General information Highly pathogenic avian influenza (HPAI)
More informationAffinity of Doripenem and Comparators to Penicillin-Binding Proteins in Escherichia coli and ACCEPTED
AAC Accepts, published online ahead of print on February 00 Antimicrob. Agents Chemother. doi:./aac.01-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationAPril PUlseNet
Issues in Brief Pulsenet: A Critical Food Safety Surveillance System Association of Public Health Laboratories APril 2010 PUlseNet A Critical Food Safety Surveillance System Public health laboratorians
More informationViral Agents of Paediatric Gastroenteritis
Viral Agents of Paediatric Gastroenteritis Dr Carl Kirkwood -------------------- Enteric Virus Research Group Murdoch Childrens Research Institute Royal Children s Hospital Victoria. WHO Collaborating
More informationMRC-Holland MLPA. Description version 14; 28 September 2016
SALSA MLPA probemix P279-B3 CACNA1A Lot B3-0816. As compared to version B2 (lot B2-1012), one reference probe has been replaced and the length of several probes has been adjusted. Voltage-dependent calcium
More informationOriginal Article The programmed death-1 gene polymorphism (PD-1.5 C/T) is associated with non-small cell lung cancer risk in a Chinese Han population
Int J Clin Exp Med 2014;7(12):5832-5836 www.ijcem.com /ISSN:1940-5901/IJCEM0002117 Original Article The programmed death-1 gene polymorphism (PD-1.5 C/T) is associated with non-small cell lung cancer risk
More informationReport of Typing & Antimicrobial Susceptibilities of Isolates Causing Invasive Pneumococcal Disease in Ireland,
Report of Typing & Antimicrobial Susceptibilities of Isolates Causing Invasive Pneumococcal Disease in Ireland, 2011-2013 1. Background Streptococcus pneumoniae is a major cause of life-threatening infections
More informationEnhancing animal health security and food safety in organic livestock production
Enhancing animal health security and food safety in organic livestock production Proceedings of the 3 rd SAFO Workshop 16-18 September 2004, Falenty, Poland Edited by M. Hovi, J. Zastawny and S. Padel
More informationRESEARCH NOTE. 86 Clinical Microbiology and Infection, Volume 12 Number 1, January 2006
86 Clinical Microbiology and Infection, Volume 12 Number 1, January 2006 REFERENCES 1. Archer GL. Staphylococcus aureus: a well-armed pathogen. Clin Infect Dis 1998; 26: 1179 1181. 2. Barenfanger J, Drake
More informationPrevalence of HPV-16 genomic variant carrying a 63-bp duplicated sequence within the E1 gene in Slovenian women
Prevalence of HPV-16 genomic variant carrying a 63-bp duplicated sequence within the E1 gene in Slovenian women K E Y WORDS HPV-16, cervical cancer, E6-T350G variant A BSTRACT High-risk HPV, particularly
More informationSubclinical mastitis caused by Mycoplasma-like bacteria in in dairy cattle in South Australia
Friday, August 12, 2016 Subclinical mastitis caused by Mycoplasma-like bacteria in in dairy cattle in South Australia Abd Al-Bar Al-Farha a1642419@adelaide.edu.au Mycoplasma Smallest bacteria Mollicutes
More informationHuman leukocyte antigen-b27 alleles in Xinjiang Uygur patients with ankylosing spondylitis
Human leukocyte antigen-b27 alleles in Xinjiang Uygur patients with ankylosing spondylitis H.-Y. Zou, W.-Z. Yu, Z. Wang, J. He and M. Jiao Institute of Clinical Medicine, Urumqi General Hospital, Lanzhou
More informationSurveillance of antimicrobial susceptibility of Enterobacteriaceae pathogens isolated from intensive care units and surgical units in Russia
Feb. 2016 THE JAPANESE JOURNAL OF ANTIBIOTICS 69 1 41 41 Surveillance of antimicrobial susceptibility of Enterobacteriaceae pathogens isolated from intensive care units and surgical units in Russia IRINA
More informationHaemophilus influenzae and its invisibility cloak. Anna Strain Virology Supervisor/VPD Reference Center Coordinator June 5, 2018
Haemophilus influenzae and its invisibility cloak Anna Strain Virology Supervisor/VPD Reference Center Coordinator June 5, 2018 Haemophilus influenzae Gram negative aerobic coccobacilli Pfeiffer s Bacillus-
More informationMRC-Holland MLPA. Description version 30; 06 June 2017
SALSA MLPA probemix P081-C1/P082-C1 NF1 P081 Lot C1-0517, C1-0114. As compared to the previous B2 version (lot B2-0813, B2-0912), 11 target probes are replaced or added, and 10 new reference probes are
More informationResistance to new anti-grampositive. Roland Leclercq, Microbiology, CHU Cote de Nacre, Caen, France
Resistance to new anti-grampositive agents Roland Leclercq, Microbiology, CHU Cote de Nacre, Caen, France Recently available antimicrobials against MDR Gram-positive infections Cyclic lipopeptide: daptomycin
More informationCampylobacter: the actual status and control options
Campylobacter: the actual status and control options Prof. Jaap A. Wagenaar, DVM, PhD Dept. Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands
More informationORIGINAL ARTICLE /j x
ORIGINAL ARTICLE 10.1111/j.1469-0691.2004.00869.x Invasive Streptococcus pneumoniae from Portugal: implications for vaccination and antimicrobial therapy I. Serrano, M. Ramirez, the Portuguese Surveillance
More informationfor Microbiology Novos programas de Controlo de Qualidade Externo: desenvolvimento e perspectivas 15 and 16 October 2008 Biognóstica - Portugal
for Microbiology Novos programas de Controlo de Qualidade Externo: desenvolvimento e perspectivas Overview Development of new schemes Molecular detection of mycobacteria Introduced as a new scheme in 2007
More informationA Guideline for the Molecular Typing of Canadian Epidemic Methicillin-Resistant Staphylococcus aureus Using spa Typing.
A Guideline for the Molecular Typing of Canadian Epidemic Methicillin-Resistant Staphylococcus aureus Using spa Typing. George R. Golding National Microbiology Laboratory Winnipeg, Manitoba AMMI-CACMID
More informationEstimates for the mutation rates of spoligotypes and VNTR types of Mycobacterium tuberculosis
Estimates for the mutation rates of spoligotypes and VNTR types of Mycobacterium tuberculosis Josephine F. Reyes and Mark M. Tanaka November 30, 2009 1/21 Understanding diversity in bacterial pathogens
More informationAldehyde Dehydrogenase-2 Genotype Detection in Fingernails among Non-alcoholic Northeastern Thai Population and Derived Gene Frequency
ScienceAsia 28 (2002) : 99-103 Aldehyde Dehydrogenase-2 Genotype Detection in Fingernails among Non-alcoholic Northeastern Thai Population and Derived Gene Frequency Paiboon Mongconthawornchai a, *, Somsong
More informationReceived 21 April 1997/Returned for modification 30 June 1997/Accepted 28 August 1997
JOURNAL OF CLINICAL MICROBIOLOGY, Dec. 1997, p. 3258 3263 Vol. 35, No. 12 0095-1137/97/$04.00 0 Copyright 1997, American Society for Microbiology Comparison of Agar Dilution, Broth Microdilution, E-Test,
More informationSALSA MLPA probemix P241-D2 MODY mix 1 Lot D As compared to version D1 (lot D1-0911), one reference probe has been replaced.
mix P241-D2 MODY mix 1 Lot D2-0413. As compared to version D1 (lot D1-0911), one reference has been replaced. Maturity-Onset Diabetes of the Young (MODY) is a distinct form of non insulin-dependent diabetes
More information