Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Luton and Dunstable University Hospital NHS Foundation Trust Lewsey Road Luton Bedfordshire LU4 0DZ Contact: Pauline Philip Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only HUMAN BODY FLUIDS DETAIL OF ACCREDITATION General Bacteriology Swabs from eyes, wounds, nose, throat, ears, genital sites, Respiratory fluids (sputum, bronchial washings) Swabs from nose, perineum and wound sites Fluids from CSF, ascetic and peritoneal fluids Urine Urine Nasopharyngeal aspirates Urine Isolation of MRSA Quantification of white and red blood cells and bacteria Quantification of white and red blood cells, bacteria and epithelial cells Detection of RSV fusion protein antigen Detection of Legionella pneumophila serotype 1 antigen Manual culture using SOPs Wound Processing, ENT processing, Genital swabs Manual culture using SOP Manual culture on chromogenic agar using SOP SOP by manual microscopy, cell count Kova slide, Gram stain inverted microscopy using SOP SOP using urine ID plates, AST breakpoint plates and chromogenic media SOP with manufacturer s instructions using Binax NOW kit Binax NOW kit Assessment Manager: JF Page 1 of 6

2 General Bacteriology MaldiTOF Faeces Faeces characterisation of Salmonella, Shigella, Campylobacter & E. coli 0157 Detection of Clostridium difficile GDH antigen and toxins A & B Manual culture methods using selective agar and SOP C. diff Check 60 (ELISA) on DS2 analyser and Quik Chek complete (immunochromatography) Faeces Detection of Helicobacter pylori manufacturer s instructions for IDEIA HpStaR kit on DS2 analyser Blood cultures Detection of microbial growth and general isolation and manufacturer s instructions for BacTALERT 3D automated system, Gram film for positives Human samples Mycobacteria Liquid culture SOP BacTalert 3Dautomated system, confirmation on Cepheid by PCR SOP Bacterial cultures (isolated inhouse) Antimicrobial susceptibility testing and determination of minimum inhibitory concentration (MIC) Manual disk diffusion using SOP and SOP for in house AST break point plates, Stokes Disc diffusion. Faeces Parasitology Examination for the presence of parasites (ova, cysts, trophozoites and worms) SOP by concentration using Parasep system and manual microscopy SOP IF microscopy, ZN stain Assessment Manager: JF Page 2 of 6

3 Human samples including swabs, fluids, tissues as specified Skin scrapings and nail clippings Mycology characterisation of fungi of clinical significance SOP Manual method using SOP by direct microscopy of KOH digested samples and culture on slopes Serology Detection of: Hepatitis A IgM, total HAV Hepatitis B surface antigen Hepatitis C antibody Rubella IgM and IgG HIV antigen Syphilis antibodies CMV IgG and IgM Toxoplasma IgG Mumps IgG Measles IgG Streptolysin O antibodies Hepatitis B surface antigen Hepatitis B surface antibody Hepatitis B core antibody Rubella IgG In house documented procedures based on manufacturer s instructions SOPs in conjunction with Biomerieux Vidas Mini (immunoassay) BIomerieux manual test SOPs in conjunction with Instrumentation Laboratory Best 2000 Bioelisa, EIA Assessment Manager: JF Page 3 of 6

4 Serology Detection of: Detection of VZV antibody T.pallidum antibodies Influenza A, influenza B, adenovirus, respiratory syncytial virus (RSV), parainfluenza 1, parainfluenza 2, and parainfluenza 3 Adenoovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and respiratory syncytial virus (RSV). VZV Measles virus Adenovirus Mumps virus Hepatitis C Virus In house documented procedures based on manufacturer s instructions Launch Diamedix VZV Mast Serodia Myco 2 kit manufacturer s instruction using Millipore respiratory screen kit Millipore respiratory panel 1 Millipore varicella Zoster DFA kit Millipore DFA kit Millipore Adenovirus DFA kit Millipore Mumps IFA kit Ortho Diagnostics Best 2000 Assessment Manager: JF Page 4 of 6

5 Serology Detection of: Herpes Simplex Virus 1 & 2 ASL C.trachomatis In house documented procedures based on manufacturer s instructions Siemens Culture identification/testing kit Siemens Rapi Tex ASL Combipack 50 kit manufacturer s using Siemens Chlamydia Trachomitis DST Detection of Influenza A, Influenza B, Parainfluenza 1,2 and 3, Respiratory Syncycial Virus, Adenovirus and Metapneumovirus Enterovirus CMV Microgen respiratory Virus Screening DFA kit Microgen Enterovirus IFA kit Microgen CMV IEA kit Urines and endocervical swabs Molecular Biology Detection of: Chlamydia Trachomatis Polymerase Chain Reaction using documented in-house procedure Abbot Evo75 & TaqMan 48 and Becton Dickinson VIPER Assessment Manager: JF Page 5 of 6

6 Molecular Biology HIV viral load Hepatitis C Viral Load manufacturer s instructions Roche Cobas Ampliprep/TAQMAN 48 END Assessment Manager: JF Page 6 of 6