Influence of Host Cell Defence during Influenza Vaccine Production in MDCK Cells
|
|
- Elinor Harris
- 6 years ago
- Views:
Transcription
1 Vaccine Technology III, 07 June 2010 Puerto Vallarta/Mexico Influence of Host Cell Defence during Influenza Vaccine Production in MDCK Cells T. Frensing 1, C. Seitz 1, B. Heynisch 2 and U. Reichl 1/2 1 Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany 2 Chair of Bioprocess Engineering, Otto von Guericke University Magdeburg, Germany MAX-PLANCK-INSTITUT DYNAMIK KOMPLEXER TECHNISCHER SYSTEME MAGDEBURG
2 Influenza Virus NA (Neuraminidase) Lipid bilayer HA (Hemagglutinin) M2 (Ion channel) Orthomyxoviridea: segmented single-stranded RNA genome divided into influenza A, B and C viruses annually 3-5 million cases of severe illness worldwide and 250, ,000 deaths (WHO) best protection by vaccination annually with trivalent seasonal influenza vaccine Egg-based vaccine production M1 (matrix protein) Ulmer et al., 2006 Innate immune response in vaccine production June
3 Bioprocess Engineering Group Cell culture-based vaccine production MDCK (Madin-Darby canine kidney) cells Cytodex 1 (dextran) microcarriers -O-CHCH 2 CH 2 -N CH 2 CH 3 CH 2 CH 3 4 days of cell growth to approx. 2.0 x 10 6 cells/ml medium exchange and virus infection with MOI 25 virus propagation for 2-3 days Genzel et al., h 10 h 22 h 100 µm Innate immune response in vaccine production June
4 Influenza A/PuertoRico/8/34 Influenza virus strain PR8 variants: A/PR/8/34 RKI (Robert Koch Institute) A/PR/8/34 NIBSC (National Institute for Biological Standards and Control) PR8 variants differ in: yield replication kinetics apoptosis induction Schulze-Horsel et al., 2009 amount of differentially expressed host proteins Vester et al., 2010 Innate immune response in vaccine production June
5 1 2 3 Limitations by Innate Immune Response? 4 8 Mock Infection Mock Infection Mx1 12 Mock Infection average ratio MDCK cells Julkunen et al., Vester et al., 2009 Innate immune response in vaccine production June
6 Signalling Pathways infection at a low MOI NS1 P P P NS1 Western blots P qrt-pcr for IFN-β and cmx1 Haller et al., 2006 Innate immune response in vaccine production June
7 Interferon Induction IκBα IRF-3 IFN-mediated signalling / activation of the antiviral state relative induction relative induction time () time () PR8-NIBSC - - PR8-RKI T-flask experiments with an MOI 5; n=4 * * * relative induction * * time () Björn Heynisch Heynisch et al., 2009 submitted Innate immune response in vaccine production June
8 Interferon Induction IκBα relative induction IRF-3 relative induction T-flask T-flask MOI 5; n=4 MOI 25; n= time () * * time () * relative induction time () - - PR8-NIBSC - - PR8-RKI relative induction time () relative induction Bioreactor (DasGip) MOI 25; n= time () relative induction time () Innate immune response in vaccine production June
9 Interferon Induction Claudius Seitz IFN-β (fold induction) M1/NS1 mrna copies / 18s RNA copy Interferon induction PR8-delNS1 PR8-NIBSC PR8-RKI (NS WSN33 (NS Intracellular viral mrna PR8-delNS1 (M1) PR8-NIBSC (NS1) PR8-RKI (NS1) WSN33 (NS1) Mx1 (fold induction) MOI Antiviral state marker cmx PR8-delNS1 PR8-NIBSC PR8-RKI (NS WSN33 (NS loss of function: - Does the inhibition of host defence increase the virus titre? gain of function: - Does the activation of the antiviral state reduce the virus yield? Seitz & Frensing et al., 2010 Innate immune response in vaccine production June
10 Loss of function: viral antagonist NS1 relative expression IFN-β NS1 10% 8% 6% 4% 2% 0.2% 0.1% % delns1 NS1 IFN-β PR8-NIBSC relative expression Mx1 control NS1 100% 80% 60% 40% 10% 5% 0% delns1 cmx1 PR8-NIBSC control NS1 virus genome copies (ml -1 ) virus genome copies (ml -1 ) MOI PR8-delNS NS1 (qrt-pcr) 1.8 control (qrt-pcr) NS1 (log HA) control (log HA) PR8-NIBSC NS1 (qrt-pcr) 1.8 control (qrt-pcr) NS1 (log HA) control (log HA) but: NS1 promotes viral mrnas translation. Which NS1 function enhanced the virus titre? Innate immune response in vaccine production June log HA/100 µl log HA/100 µl
11 Loss of function: viral antagonist rp NS1 relative expression 100% 80% 60% 40% 20% 0% rp IFN IFN-β rp Mx cmx1 control rp virus genome copies (ml -1 ) MOI rp (qrt-pcr) control (qrt-pcr) rp (log HA) control (log HA) log HA/100 µl Inhibition of IFN signalling does not result in higher virus yields Innate immune response in vaccine production June
12 Gain of function: cytokine stimulation ultrafiltration 100 kda cut-off 1st infection: MOI 5 A 100 cmx1 induction 80 virus genome copies (ml -1 ) PR8-RKI MOI 25 2nd infection: MOI control (qrt-pcr) CM (qrt-pcr) control (log HA) CM (log HA) log HA/100 µl Mx1 (fold induction) Innate immune response in vaccine production June MOCK delns1 PR8-RKI PR8-NIBSC Virus replication is slowed down, but almost the same titre is reached - active virus titre (TCID50) - MOIs (25 / 025 / 0025) - PR8-NIBSC, A/WSN/33
13 Gain of function: cytokine stimulation 3.5 B/Malaysia/2506/2004 (MOI 25) 1600 IFN induction log HA / 100 µl control stimulated fold induction control stimulated cmx1 induction NS1 NS1 fold induction control stimulated Innate immune response in vaccine production June
14 Minireplicon Assay minigenome with reporter gene Pol-I promoter expression plasmids Pol-II promoter HEK 293 cells NCR FF-Luciferase (-) NCR Renilla PA PB1 FF-Luc PB2 NP vrna (-) Prof. Dr. Georg Kochs Mx luciferase assay Innate immune response in vaccine production June
15 Minireplicon Assay 140 relative activity [%] Mx 0 empty vector empty vector MxA MxA MxA mut MxA mut cmx1 cmx2 mmx1 mmx1 mut cmx1 cmx2 mmx1 mmx1 mut NP ERK2 No inhibitory effects of canine Mx proteins Seitz, C. & Frensing, T., Höper, D., Kochs, G., Reichl, U. (2010). High yields of Influenza A virus in MDCK cells are promoted by an insufficient IFN-induced antiviral state. Journal of General Virology, accepted Innate immune response in vaccine production June
16 Summary considerable IFN signalling in influenza virus infected MDCK cells no impact on final virus titres by inhibition or stimulation of IFN lack of inhibitory potential of canine Mx proteins against influenza virus IFN signalling has only a minor effect on influenza virus replication in MDCK cells, which makes these cells an ideal system for high yield vaccine production. Innate immune response in vaccine production June
17 Acknowledgment Signal transduction group: Claudius Seitz Nancy Wynserski Bianca Kaps Björn Heynisch Maria Rettkowski former students: Kristina Heinze Stefan Heldt Anja Fincke Henrike Götze Innate immune response in vaccine production June
18 Acknowledgment Bioprocess Engineering Group Prof. Dr.-Ing. Udo Reichl Cooperations: Virology Freiburg: Prof. Dr. Georg Kochs FLI Insel Riems: Dr. Dirk Höper PD. Dr. med. vet. Timm Harder Thanks for your attention! MPI-Infection Biology, Berlin: Dr. Alexander Karlas Innate immune response in vaccine production June
Impact of defective interfering particles on virus replication and antiviral host response in cell culture-based influenza vaccine production
Impact of defective interfering particles on virus replication and antiviral host response in cell culture-based influenza vaccine production Timo Frensing 1*, Antje Pflugmacher 1, Mandy Bachmann 1, Britta
More informationSupplementary Figure 1. SC35M polymerase activity in the presence of Bat or SC35M NP encoded from the phw2000 rescue plasmid.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Supplementary Figure 1. SC35M polymerase activity in the presence of Bat or SC35M NP encoded from the phw2000 rescue plasmid. HEK293T
More informationInfluenza viruses. Virion. Genome. Genes and proteins. Viruses and hosts. Diseases. Distinctive characteristics
Influenza viruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Virion Enveloped particles, quasi-spherical or filamentous Diameter 80-120 nm Envelope is derived
More informationhttp://nmhm.washingtondc.museum/collections/archives/agalleries/1918flu/ncp1603.jpg 1 https://assets-production-webvanta-com.s3-us-west- 2 2.amazonaws.com/000000/47/62/original/images/img_109_influenza/Spanish_flu_death_chart.jpg
More informationFIG S1 Examination of eif4b expression after virus infection. (A) A549 cells
Supplementary Figure Legends FIG S1 Examination of expression after virus infection. () 549 cells were infected with herpes simplex virus (HSV) (MOI = 1), and harvested at the indicated times, followed
More informationRalf Wagner Paul-Ehrlich-Institut
www.pei.de Other Assays for the Detection of Neuraminidase (NA)-Specific Antibodies Ralf Wagner Paul-Ehrlich-Institut Overview to presented assays Assay principle based on: Chemical substrates: Protein
More informationUse of Capto ViralQ for the removal of genomic DNA from influenza virus produced in MDCK cells
GE Healthcare Application note 28-9769-69 AA Vaccines Use of Capto ViralQ for the removal of genomic DNA from influenza virus produced in MDCK cells We have developed a chromatographic method to remove
More informationRegulation of cell signaling cascades by influenza A virus
The Second International Symposium on Optimization and Systems Biology (OSB 08) Lijiang, China, October 31 November 3, 2008 Copyright 2008 ORSC & APORC, pp. 389 394 Regulation of cell signaling cascades
More informationIntrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
More informationSupplementary Fig. S1. Schematic diagram of minigenome segments.
open reading frame 1565 (segment 5) 47 (-) 3 5 (+) 76 101 125 149 173 197 221 246 287 open reading frame 890 (segment 8) 60 (-) 3 5 (+) 172 Supplementary Fig. S1. Schematic diagram of minigenome segments.
More informationMultiple Anti-Interferon Actions of the Influenza A Virus NS1 Protein
JOURNAL OF VIROLOGY, July 2007, p. 7011 7021 Vol. 81, No. 13 0022-538X/07/$08.00 0 doi:10.1128/jvi.02581-06 Copyright 2007, American Society for Microbiology. All Rights Reserved. Multiple Anti-Interferon
More informationRelative activity (%) SC35M
a 125 Bat (H17N) b 125 A/WSN (H1N1) Relative activity (%) 0 75 50 25 Relative activity (%) 0 75 50 25 0 Pos. Neg. PA PB1 Pos. Neg. NP PA PB1 PB2 0 Pos. Neg. NP PA PB1 PB2 SC35M Bat Supplementary Figure
More informationConcentration and diafiltration of cell-derived, live influenza virus using 750 C hollow fiber filter cartridge
GE Healthcare Life Sciences Application note 29-928-26 AA Cross flow filtration Concentration and diafiltration of cell-derived, live influenza virus using 75 C hollow fiber filter cartridge This application
More informationPrevention and treatment of swine-origin influenza virus with interferon: an in vivo and ex vivo study
RESEARCH FUND FOR THE CONTROL OF INFECTIOUS DISEASES Prevention and treatment of swine-origin influenza virus with interferon: an in vivo and ex vivo study JM Nicholls *, RWY Chan, E Fish K e y M e s s
More informationNew Small and Versatile Reporter Technologies for Challenging Applications in Virology. Robert Brazas, Ph.D.
New Small and Versatile Reporter Technologies for Challenging Applications in Virology Robert Brazas, Ph.D. Presentation Overview Small size of NanoLuc Luciferase is advantageous Agenda Building a better
More informationTechnology Overview. Summary
Live Attenuated Influenza Vaccines with Altered NS1 Technology Overview Summary Transformative Technology: Live attenuated influenza vaccines (LAIVs) with precise, genetically stable truncations of the
More informationPatricia Fitzgerald-Bocarsly
FLU Patricia Fitzgerald-Bocarsly October 23, 2008 Orthomyxoviruses Orthomyxo virus (ortho = true or correct ) Negative-sense RNA virus (complementary to mrna) Five different genera Influenza A, B, C Thogotovirus
More informationProduction of Avian Influenza H5N1 Virus Using the TideCell Bioreactor System
Production of Avian Influenza H5N1 Virus Using the TideCell Bioreactor System www.bioreactorsciences.com A Production Case Study Using MDCK Cell Line Comparison Study Due to significant differences in
More informationMultifunctional Adaptive NS1 Mutations Are Selected upon Human Influenza Virus Evolution in the Mouse
Multifunctional Adaptive NS1 Mutations Are Selected upon Human Influenza Virus Evolution in the Mouse Nicole E. Forbes 1,2, Jihui Ping 1,2, Samar K. Dankar 1,2, Jian-Jun Jia 1,2, Mohammed Selman 1,2, Liya
More informationCrucial role for human Toll-like receptor 4 in the development of contact allergy to nickel
Supplementary Figures 1-8 Crucial role for human Toll-like receptor 4 in the development of contact allergy to nickel Marc Schmidt 1,2, Badrinarayanan Raghavan 1,2, Verena Müller 1,2, Thomas Vogl 3, György
More informationViral genetic determinants of h5n1 influenza viruses that contribute to cytokine dysregulation
Title Viral genetic determinants of h5n1 influenza viruses that contribute to cytokine dysregulation Author(s) Mok, KP; Wong, CHK; Cheung, CY; Chan, MC; Lee, SMY; Nicholls, JM; Guan, Y; Peiris, JSM Citation
More informationDoctoral Degree Program in Marine Biotechnology, College of Marine Sciences, Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei,
Cyclooxygenase 2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents Chun-Kuang Lin 1,2, Chin-Kai Tseng 3,4, Yu-Hsuan Wu 3,4, Chih-Chuang Liaw 1,5, Chun-
More informationCharacterization of Uncultivable Bat Influenza Virus Using a Replicative Synthetic Virus
Characterization of Uncultivable Bat Influenza Virus Using a Replicative Synthetic Virus Bin Zhou 1. a, Jingjiao Ma 2., Qinfang Liu 2., Bhupinder Bawa 2, Wei Wang 1, Reed S. Shabman 1, Michael Duff 2,
More informationSupplementary Figure 1. Prevalence of U539C and G540A nucleotide and E172K amino acid substitutions among H9N2 viruses. Full-length H9N2 NS
Supplementary Figure 1. Prevalence of U539C and G540A nucleotide and E172K amino acid substitutions among H9N2 viruses. Full-length H9N2 NS nucleotide sequences (a, b) or amino acid sequences (c) from
More informationChallenges and Solutions for the Next Generation of Vaccines: Jonathan Liu Luis Maranga Sachin Mani Richard Schwartz
Challenges and Solutions for the Next Generation of Vaccines: Development of fcell llculture-based dlive Attenuated t Influenza Vaccine VACCINE TECHNOLOGY II June 1, 2008 Albufeira (near Faro), Algarve,
More informationInfluenza Virus Genotypes Circulating In Central Greece During And Vaccine Strain Match
ISPUB.COM The Internet Journal of Microbiology Volume 13 Number 1 Influenza Virus Genotypes Circulating In Central Greece During 2012-2014 And Vaccine Strain Match E Plakokefalos, A Vontas, Z Florou, G
More informationChallenges in Vaccine Production and Rapid Scale up to Meet Emerging Pandemic Threats
Challenges in Vaccine Production and Rapid Scale up to Meet Emerging Pandemic Threats Susan Dana Jones, Ph.D. BioProcess Technology Consultants, Inc. BIO 2009 Process Zone Theater Atlanta, GE May 20, 2009
More informationReverse Genetics of RNA Viruses
Reverse Genetics of RNA Viruses Reverse Genetics (RG) he creation of a virus with a fulllength copy of the viral genome he most powerful tool in modern virology RG of RNA viruses Generation or recovery
More informationHepatitis B Antiviral Drug Development Multi-Marker Screening Assay
Hepatitis B Antiviral Drug Development Multi-Marker Screening Assay Background ImQuest BioSciences has developed and qualified a single-plate method to expedite the screening of antiviral agents against
More informationRole for autophagy in cellular response to influenza virus infection
RESEARCH FUND FOR THE CONTROL OF INFECTIOUS DISEASES Role for autophagy in cellular response to influenza virus infection AHY Law, DCW Lee, TYY Leon, ASY Lau * K e y M e s s a g e s 1. A differential induction
More informationCompeting co-infections of LP and HP AIV H7N7
Competing co-infections of LP and HP AIV H7N7 Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health Suedufer 10, 17493 Greifswald-Island of Riems, Germany Annika Graaf, Timm Harder
More informationMin Levine, Ph. D. Influenza Division US Centers for Disease Control and Prevention. June 18, 2015 NIBSC
Workshop on Immunoassay Standardization for Universal Flu Vaccines Min Levine, Ph. D. Influenza Division US Centers for Disease Control and Prevention June 18, 2015 NIBSC 1 Multiple Immune Mechanisms Contribute
More informationPersistent Infection of MDCK Cells by Influenza C Virus: Initiation and Characterization
J. gen. Virol. (199), 70, 341-345. Printed in Great Britain 341 Key words: influenza C virus/interferon/persistent infection Persistent Infection of MDCK Cells by Influenza C Virus: Initiation and Characterization
More informationThe X protein of Borna disease virus serves essential functions in ACCEPTED. Department of Virology, University of Freiburg, Freiburg, Germany
JVI Accepts, published online ahead of print on 11 April 07 J. Virol. doi:.1128/jvi.02468-06 Copyright 07, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
More informationSegments 7 codes for M1 and M2 proteins (Matrix proteins)
One Flu over the Cuckoo s nest Dr Rachel Jones Department of Virology NPHS Microbiology Cardiff Whistlestop tour through Flu The virus Some history The Novel Virus Recent events The disease The control
More informationSupporting Information
Supporting Information Yen et al. 10.1073/pnas.1111000108 SI Materials and Methods Cells. Madin Darby canine kidney (MDCK) cells and human embryonic kidney 293T cells were obtained from the American Type
More informationavian influenza vaccines Rutger van Wielink
Cell culture based production of avian influenza vaccines Rutger van Wielink Cell culture based production of avian influenza vaccines Rutger van Wielink Thesis committee Promotors Prof. dr. ir. R.H. Wijffels
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/8/406/ra126/dc1 Supplementary Materials for The microrna mir-485 targets host and influenza virus transcripts to regulate antiviral immunity and restrict viral
More informationSupplementary Figure 1 Weight and body temperature of ferrets inoculated with
Supplementary Figure 1 Weight and body temperature of ferrets inoculated with A/Anhui/1/2013 (H7N9) influenza virus. (a) Body temperature and (b) weight change of ferrets after intranasal inoculation with
More informationFlu, Avian Flu and emerging aspects (H1N1 resistance)
EU-CIS Seminar New trends in Infectious Diseases 26 28 November 2008 / Lyon, France Flu, Avian Flu and emerging aspects (H1N1 resistance) Pr. Florence MORFIN FRE 3011 Université Lyon 1 - CNRS Laboratory
More informationInfluenza surveillance and pandemic preparedness - a global challenge Anne Kelso
Influenza surveillance and pandemic preparedness - a global challenge Anne Kelso WHO Collaborating Centre for Reference and Research on Influenza Melbourne, Australia Three global health challenges 243
More informationExploring synergies between academia and vaccine manufacturers: a pilot study on how to rapidly produce vaccines to combat emerging pathogens
Clin Chem Lab Med 2012;50(7):1275 1279 2012 by Walter de Gruyter Berlin Boston. DOI 10.1515/cclm-2011-0650 Exploring synergies between academia and vaccine manufacturers: a pilot study on how to rapidly
More informationIII. What are the requirements for taking and passing this course?
1 Molecular Virology Lecture # 1: Course Introduction I. Instructor and Background Dr. Richard Kuhn rjkuhn@bragg.bio.purdue.edu B-129 Lilly Hall 494-1164 Office Hours - Wednesday 10:30-11:30 II. Objective:
More informationInfluenzavirus. Influenzavirus 10/27/15. Lord Randolphs report from Queen Marys trip to Scotland in 1562
A disease under the influence of the stars or the moon. Lord Randolphs report from Queen Marys trip to Scotland in 1562 She fell acquaintance with a new disease which passed through her whole court neither
More informationDevelopment of safe and immunogenic reassortant viruses with 5:3 genotype for live attenuated influenza vaccine
Development of safe and immunogenic reassortant viruses with 5:3 genotype for live attenuated influenza vaccine Irina Isakova-Sivak, PhD Institute of Experimental Medicine, Saint Petersburg, Russia The
More informationSupporting Information
Supporting Information Valkenburg et al. 10.1073/pnas.1403684111 SI Materials and Methods ELISA and Microneutralization. Sera were treated with Receptor Destroying Enzyme II (RDE II, Accurate) before ELISA
More informationModeling the Antigenic Evolution of Influenza Viruses from Sequences
Modeling the Antigenic Evolution of Influenza Viruses from Sequences Taijiao Jiang Center of Systems Medicine, Chinese Academy of Medical Sciences Suzhou Institute of Systems Medicine October 8-10, 2015.
More informationEnvelope e_ :------, Envelope glycoproteins e_ ~ Single-stranded RNA ----, Nucleocapsid
Virus Antib(tdies Your Expertise, Our Antibodies, Accelerated Discovery. Envelope e_---------:------, Envelope glycoproteins e_-------1~ Single-stranded RNA ----, Nucleocapsid First identified in 1989,
More informationReoviruses. Virion. Genome. Genes and proteins. Viruses and hosts. Diseases. Distinctive characteristics
Reoviruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Virion Naked icosahedral capsid (T=13), diameter 60-85 nm Capsid consists of two or three concentric protein
More informationOverview: Chapter 19 Viruses: A Borrowed Life
Overview: Chapter 19 Viruses: A Borrowed Life Viruses called bacteriophages can infect and set in motion a genetic takeover of bacteria, such as Escherichia coli Viruses lead a kind of borrowed life between
More informationCYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION
CYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION What is Cytokine? Secreted popypeptide (protein) involved in cell-to-cell signaling. Acts in paracrine or autocrine fashion through specific cellular receptors.
More informationBroadly protective influenza vaccines for pandemic preparedness. Suresh Mittal Department of Comparative Pathobiology Purdue University
Broadly protective influenza vaccines for pandemic preparedness Suresh Mittal Department of Comparative Pathobiology Purdue University Influenza A Virus Orthomyxovirus Consist of s/s (-) sense RNA 8 segments
More informationSupplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
More informationSUPPLEMENTAL INFORMATIONS
1 SUPPLEMENTAL INFORMATIONS Figure S1 Cumulative ZIKV production by testis explants over a 9 day-culture period. Viral titer values presented in Figure 1B (viral release over a 3 day-culture period measured
More informationAbstract: I. A ims Aim 1:
Abstract: Previous work from our laboratory demonstrated that obese mice have alterations in antiviral cytokine gene expression when infected with the influenza virus. Since these cytokines play a major
More informationThe Chimpanzee Model Of HCV: Antiviral Therapy
The Chimpanzee Model Of HCV: Antiviral Therapy Antiviral Therapies in Chimpanzees PEG IFN + Ribavirin STAT C with DAA Protease Inhibitors Nucleoside analogues Polymerase Inhibitors NS5A Inhibitors Immunomodulators
More informationThe nonstructural (NS1) protein of influenza A virus is an antagonist
Mutations in the M-Gene Segment Can Substantially Increase Replication Efficiency of NS1 Deletion Influenza A Virus in MDCK Cells R. van Wielink, a,b M. M. Harmsen, a D. E. Martens b B. P. H. Peeters,
More informationApplication of Reverse Genetics to Influenza Vaccine Development
NIAID Application of Reverse Genetics to Influenza Vaccine Development Kanta Subbarao Laboratory of Infectious Diseases NIAID, NIH Licensed Vaccines for Influenza Principle: Induction of a protective
More informationCleavage and polyadenylation specificity factor (CPSF), elongation initiation factor 4GI (eif4gi),
JVI Accepts, published online ahead of print on 29 April 2009 J. Virol. doi:10.1128/jvi.02323-08 Copyright 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
More informationDevelopment of a Novel Recombinant Influenza Vaccine in Insect Cells
Development of a Novel Recombinant Influenza Vaccine in Insect Cells Clifton McPherson New Cells for New Vaccines II September 18, 2007 cmcpherson@proteinsciences.com New Cell for New Vaccines II Topics:
More informationInfluenza viruses are classified as members of the family
Rewiring the RNAs of influenza virus to prevent reassortment Qinshan Gao a and Peter Palese a,b,1 Departments of a Microbiology and b Medicine, Mount Sinai School of Medicine, New York, NY 10029 Contributed
More informationReassortment of influenza A virus genes linked to PB1 polymerase gene
International Congress Series 1263 (2004) 714 718 Reassortment of influenza A virus genes linked to PB1 polymerase gene Jean C. Downie* www.ics-elsevier.com Centre for Infectious Diseases and Microbiology,
More informationMedical Virology. Herpesviruses, Orthomyxoviruses, and Retro virus. - Herpesviruses Structure & Composition: Herpesviruses
Medical Virology Lecture 2 Asst. Prof. Dr. Dalya Basil Herpesviruses, Orthomyxoviruses, and Retro virus - Herpesviruses Structure & Composition: Herpesviruses Enveloped DNA viruses. All herpesviruses have
More informationSupplementary information. MARCH8 inhibits HIV-1 infection by reducing virion incorporation of envelope glycoproteins
Supplementary information inhibits HIV-1 infection by reducing virion incorporation of envelope glycoproteins Takuya Tada, Yanzhao Zhang, Takayoshi Koyama, Minoru Tobiume, Yasuko Tsunetsugu-Yokota, Shoji
More informationInfluenza Genome Sequencing Project Proposal
Date of Proposal (MM/DD/YY): 06/13/12 TITLE: Role of the Untranslated Regions of the Influenza A Virus Replication and Vaccines Purpose/Objective-please provide a brief one to two paragraph description
More informationImmunobiotics: Cloudbreak Antibody Drug Conjugates (ADC) Les Tari, PhD VP Discovery
Immunobiotics: Cloudbreak Antibody Drug Conjugates (ADC) Les Tari, PhD VP Discovery Cidara s pipeline addresses multiple unmet needs Program Indication Discovery IND- enable Ph 1 Ph 2 Ph 3 REZAFUNGIN IV
More informationINFLUENZA VIRUS. INFLUENZA VIRUS CDC WEBSITE
INFLUENZA VIRUS INFLUENZA VIRUS CDC WEBSITE http://www.cdc.gov/ncidod/diseases/flu/fluinfo.htm 1 THE IMPACT OF INFLUENZA Deaths: PANDEMICS 1918-19 S p a n is h flu 5 0 0,0 0 0 U S 2 0,0 0 0,0 0 0 w o rld
More informationViral reproductive cycle
Lecture 29: Viruses Lecture outline 11/11/05 Types of viruses Bacteriophage Lytic and lysogenic life cycles viruses viruses Influenza Prions Mad cow disease 0.5 µm Figure 18.4 Viral structure of capsid
More informationCover Page. The handle holds various files of this Leiden University dissertation
Cover Page The handle http://hdl.handle.net/1887/35908 holds various files of this Leiden University dissertation Author: Soema, Peter Title: Formulation of influenza T cell peptides : in search of a universal
More informationPermissible Variation in the 39 Non-Coding Region of the Haemagglutinin Genome Segment of the H5N1 Candidate Influenza Vaccine Cirus NIBRG-14
Permissible Variation in the 39 Non-Coding Region of the Haemagglutinin Genome Segment of the H5N1 Candidate Influenza Vaccine Cirus NIBRG-14 Rachel E. Johnson 1, Michelle Hamill 2, Ruth Harvey 1, Carolyn
More informationSupplementary Fig. 1. Delivery of mirnas via Red Fluorescent Protein.
prfp-vector RFP Exon1 Intron RFP Exon2 prfp-mir-124 mir-93/124 RFP Exon1 Intron RFP Exon2 Untransfected prfp-vector prfp-mir-93 prfp-mir-124 Supplementary Fig. 1. Delivery of mirnas via Red Fluorescent
More informationTargeted disruption of influenza A virus hemagglutinin in genetically modified. mice reduces viral replication and improves disease outcome
Supplementary Information Targeted disruption of influenza A virus hemagglutinin in genetically modified mice reduces viral replication and improves disease outcome Song Wang 1#, Chao Chen 1#, Zhou Yang
More informationRespiratory Viruses. Respiratory Syncytial Virus
Adam Ratner, MD Respiratory Viruses Respiratory viruses are among the most common causes of disease throughout life. Often mild and self-limited, they are still associated with tremendous economic and
More informationPlasmid-Driven Formation of Influenza Virus-Like Particles
JOURNAL OF VIROLOGY, Jan. 2000, p. 547 551 Vol. 74, No. 1 0022-538X/00/$04.00 0 Copyright 2000, American Society for Microbiology. All Rights Reserved. Plasmid-Driven Formation of Influenza Virus-Like
More informationph1n1 H3N2: A Novel Influenza Virus Reassortment
ph1n1 H3N2: A Novel Influenza Virus Reassortment Jonathan Gubbay Medical Microbiologist Public Health Laboratory Public Health Ontario June 16, 2011 ph1n1 H3N2 Reassortment: Talk Overview Explain strain
More information10th International Rotavirus Symposium Bangkok, Thailand
Rotavirus Host Range Restriction and Innate Immunity: Mechanisms of Vaccine Attenuation Harry Greenberg MD Stanford University 10th International Rotavirus Symposium Bangkok, Thailand 09/19/12 B dsrna
More informationProposed 1 st IS for Hepatitis E Virus RNA WHO/BS/
www.pei.de Proposed 1 st IS for Hepatitis E Virus RNA WHO/BS/09.2126 S. Baylis, Division of Virology, Paul-Ehrlich-Institut, Langen, Germany SoGAT XXII 14 th -15 th April 2011, Rome, Italy Presented at:
More informationTitle: Cytosolic DNA-mediated, STING-dependent pro-inflammatory gene. Fig. S1. STING ligands-mediated signaling response in MEFs. (A) Primary MEFs (1
1 Supporting Information 2 3 4 Title: Cytosolic DNA-mediated, STING-dependent pro-inflammatory gene induction necessitates canonical NF-κB activation through TBK1 5 6 Authors: Abe et al. 7 8 9 Supporting
More informationOxford Expression Technologies Ltd
Oxford Expression Technologies Ltd Founded in 2007 as a spin out from Oxford Brookes University and Natural Environment Research Council Technology based on the insect baculovirus expression vectors (BEVs)
More informationAndrea Hillesheim, Carolin Nordhoff, Yvonne Boergeling, Stephan Ludwig and Viktor Wixler *
Hillesheim et al. Cell Communication and Signaling 2014, 12:29 RESEARCH Open Access β-catenin promotes the type I IFN synthesis and the IFN-dependent signaling response but is suppressed by influenza A
More informationUnité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr. Roux, PARIS Cedex 15, France
Virology 345 (2006) 73 87 www.elsevier.com/locate/yviro Recombinant influenza A viruses harboring optimized dicistronic NA segment with an extended native 5V terminal sequence: Induction of heterospecific
More informationGlobal Pandemic Preparedness Research Efforts. Klaus Stöhr. WHO Global Influenza Programme. Today
Global Pandemic Preparedness Research Efforts Klaus Stöhr 3 Today Medium-term applied research linked to medical and public health interventions addressing the current pandemic situation in Asia Natural
More informationEvolution of influenza
Evolution of influenza Today: 1. Global health impact of flu - why should we care? 2. - what are the components of the virus and how do they change? 3. Where does influenza come from? - are there animal
More informationBlocking Interhost Transmission of Influenza Virus by Vaccination in the Guinea Pig Model
JOURNAL OF VIROLOGY, Apr. 2009, p. 2803 2818 Vol. 83, No. 7 0022-538X/09/$08.00 0 doi:10.1128/jvi.02424-08 Copyright 2009, American Society for Microbiology. All Rights Reserved. Blocking Interhost Transmission
More informationInfection dynamics of novel influenza A viruses isolated from Australian pigs
Infection dynamics of novel influenza A viruses isolated from Australian pigs Joanne Taylor PhD Candidate Supervisors: Dr Frank Wong, Dr David Williams, Dr John Bingham, Dr Joanne Meers 3 rd December 2015
More informationSUPPLEMENT ARTICLE. Valeria Cannas, Gian Luca Daino, Angela Corona, Francesca Esposito, and Enzo Tramontano
SUPPLEMENT ARTICLE A Luciferase Reporter Gene Assay to Measure Ebola Virus Viral Protein 35 Associated Inhibition of Double-Stranded RNA Stimulated, Retinoic Acid Inducible Gene 1 Mediated Induction of
More informationAbstract. preventable through vaccination. Most vaccines available in the United States use an
Abstract WHITE, STEPHEN EVANS. Characterization of the Freeze/Thaw Stability of a Cell Culture-Derived Influenza A/PR/8/34 (H1N1) Virus Stock and the Production Impact thereof. (Under the direction of
More informationINDUCTION OF APOPTOSIS IN MDCK, RK13, AND NEURO-2A CELLS INFECTED WITH EQUINE INFLUENZA VIRUS
Bull Vet Inst Pulawy 57, 3-7, 2013 DOI: 10.2478/bvip-2013-0001 INDUCTION OF APOPTOSIS IN MDCK, RK13, AND NEURO-2A CELLS INFECTED WITH EQUINE INFLUENZA VIRUS MAŁGORZATA KWAŚNIK, WOJCIECH ROŻEK, AND JAN
More informationhemagglutinin and the neuraminidase genes (RNA/recombinant viruses/polyacrylamide gel electrophoresis/genetics)
Proc. Natl. Acad. Sci. USA Vol. 73, No. 6, pp. 242-246, June 976 Microbiology Mapping of the influenza virus genome: Identification of the hemagglutinin and the neuraminidase genes (RNA/recombinant viruses/polyacrylamide
More informationInfluenza. By Allison Canestaro-Garcia. Disease Etiology:
Influenza By Allison Canestaro-Garcia Disease Etiology: The flu is an infectious disease caused by a subset of viruses of the family Orthomyxoviridae. There are 7 different viruses in this family, four
More informationHuman Influenza. Dr. Sina Soleimani. Human Viral Vaccine Quality Control 89/2/29. November 2, 2011 HVVQC ١
Human Influenza Dr. Sina Soleimani Human Viral Vaccine Quality Control 89/2/29 November 2, 2011 HVVQC ١ Presentation outline 1. Introduction 2. Virology 3. Classification 4. Hosts 5. Antigenic Specifications
More informationInfluenza virus capture using membrane chromatography: Improving selectivity by matrix design and pseudo-affinity ligand interactions
Engineering Conferences International ECI Digital Archives Vaccine Technology VII Proceedings 6-17-2018 Influenza virus capture using membrane chromatography: Improving selectivity by matrix design and
More informationInfluenza 2009: Not Yet The Perfect Storm
Influenza 2009: Not Yet The Perfect Storm What s needed for a pandemic strain? Novel virus (little to no immunity) Capable of causing disease in humans Highly pathogenic / virulent Capable of sustained
More informationNOTES. The Naturally Attenuated Kunjin Strain of West Nile Virus Shows Enhanced Sensitivity to the Host Type I Interferon Response
JOURNAL OF VIROLOGY, June 2011, p. 5664 5668 Vol. 85, No. 11 0022-538X/11/$12.00 doi:10.1128/jvi.00232-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. NOTES The Naturally Attenuated
More informationAGAINST VIRAL INFECTIONS. Identify the types of immunity involve in the mechanisms of protection against viral infections.
LECTURE: 02 Title: THE IMMUNOLOGICAL PROTECTIVE MECHANISMS AGAINST VIRAL INFECTIONS LEARNING OBJECTIVES: The student should be able to: Identify the types of immunity involve in the mechanisms of protection
More informationTOWARDS A UNIVERSAL INFLUENZA VIRUS VACCINE
TOWARDS A UNIVERSAL INFLUENZA VIRUS VACCINE Peter Palese Icahn School of Medicine at Mount Sinai New York OPTIONS IX 8-26-16 ISIRV - Options IX for the Control of Influenza Peter Palese, PhD Professor
More informationUpdate on influenza monitoring and vaccine development
Update on influenza monitoring and vaccine development Annette Fox WHO Collaborating Centre for Reference and Research on Influenza at The Peter Doherty Institute for Infection and Immunity 1 Outline Why
More informationAcute respiratory illness This is a disease that typically affects the airways in the nose and throat (the upper respiratory tract).
Influenza glossary Adapted from the Centers for Disease Control and Prevention, US https://www.cdc.gov/flu/glossary/index.htm and the World Health Organization http://www.wpro.who.int/emerging_diseases/glossary_rev_sept28.pdf?ua=1
More informationnumbe r Done by Corrected by Doctor
numbe r 5 Done by Mustafa Khader Corrected by Mahdi Sharawi Doctor Ashraf Khasawneh Viral Replication Mechanisms: (Protein Synthesis) 1. Monocistronic Method: All human cells practice the monocistronic
More informationPassive surveillance of swiavs in France
SIV group technical meeting Paris, December 3 rd -4 th 2015 Passive surveillance of swiavs in France Gaëlle Simon Swine Virology Immunology Unit National Reference Laboratory for Swine Influenza Ploufragan,
More information