Patrick L. Graham, DVM, MS
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1 MJ PRRS Vaccine: Field efficacy Patrick L. Graham, DVM, MS Peggy Anne Hawkins, DVM, MS
2 Farm Introduction Fall /5 year old sow farm 6400 sows 2 previous PRRSV outbreaks Serum therapy Sows and GDU PRRS elimination!!
3 Elimination Versus Control PRRSV Elimination New animal flow New personnel flow Strict biosecurity Poor quality pigs Disgruntled owners, workers and higher costs PRRSV Control Keep it simple one goal Improve the numberof quality pigs being weaned
4 Chain of Events Third quarter 2008 Production was poor and there were too many low value pigs Week 44 and 48 of 2008 Attempt control with killed PRRS vaccine Production got worse by week Week 18 of 2009 Started using MJ PRRS vaccine By week 22 production improved ddramatically
5 Live Born Stillborn Mummies Killed vaccine Serum MJPRRS Vaccine
6 Pre wean Mortality % Pigs weaned per sow Serum Killed Vaccine MJPRRSV Vaccine
7 Farrowing Rate % Serum MJPRRS Vaccine
8 What is MJPRRS Vaccine? Autogenous Inactivated MJPRRS Viral Antigen Concentrate Vaccine Field strain virus Grown under laboratory conditions High yields of free form structural proteins Viral antigen components Optimize level of ORF5 antigen extracts KILLED Mixed with adjuvant Single or Multiple strains in each bottle
9 Autogenous Biologics in Non Adjacent Herds at Risk 9 CFR The administrator may authorize preparation of an autogenous biologic for use in herds which arenot adjacent to the herd of origin, but which s/he considers to be at risk of infection with the same microorganism(s) Epidemiological Links (Attending Veterinarian) Virus Group; Vet services; Source herd; Genetics; Stud; Pig flow; Feed; Manure; Trucking; Rendering; Packer
10 Virus Group Technology Virus grouping is very important Send virus sequence report (paper/electronic) to MJ Biologics Review sequence and determine the GROUP Major Group: D and S Minor Group: 1 to 8 D1 to D8 and S1 to S8 Group plus epidemiological profile may determine which vaccine is right for your herd Herd derived autogenous Non adjacent, herd at risk derived autogenous
11 Exposure to Live Virus Pigs must be exposed to live virus Natural exposure Serum Ingelvac PRRS ATP Ingelvac PRRS MLV Replacement gilt strategy Sow herd strategy Wean pig strategy **Ingelvac is a registered trademark of Boehringer Ingelheim Vetmedica
12 Literature Support The effect of killed and/or MLV vaccination on exposure to late term pregnant gilts D. L. Reicks et al. (Allen D. Leman Swine Conference, 2010) Only full value pigs were considered at weaning % total born weaned Positive control: 66% ATP MJPRRS: 94% (p= 0.047) [More full value pigs] ATP Only: 78% (p=0.852) It is important to prime with live virus
13 Literature Support (Cont.) Micro dissecting thepathogenesis and immune response of PRRSV infection paves the way for more effective PRRS vaccines H.J. Nauwynck, et al. (ISU Swine Disease Conference, 2010) Author noted that the next breakthrough in PRRSV control would be with inactivated (killed) vaccine technology. Vaccines will be inactivated during growth phase to conserve viral binding ligands
14 Farm Update PCR positive with New Strain RFLP from 1? 4 to Late stage abortions Sows off feed Group change from D4 to D6 D4 vaccine used through 10/28/10 (D2,3,4,5 S1) D6 vaccine used at 8 and 3 weeks prefarrow after whole herd vaccination with D2 on The 2 strains different by 12 amino acids and The 2 strains different by 12 amino acids and 98.3% homologous
15 4 th Quarter 2010 and 1 st Quarter 2011 Time Live Born Stillborn Mummies PWM Pigs Farrow Frame % % (%) Weaned/ Rate % sow 3 rd Q ' n/a Wk 4 8 '09 Wk '09 Wk ' rd Q' th Q ' st Q '
16 MJPRRS vaccine Increase pigs produced d Conclusion 70% good pigs to 96% good pigs 20% marginal pigs to 3% marginal pigs 10% no value pigs to 1% "off" pigs Performance of pigs in nursery and finish Exceptional New Strain has increased losses in some cases Pigs to be weaned are tested every 4 weeks by PCR Stayed 100% positive on PCR through Jan Most recent testing: PRRSV Negative
17
18 Questions
Porcine Reproductive and Respiratory Syndrome Virus
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