ON-GOING STUDIES ON VTEC IN SWEDEN

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1 NATIONAL VETERINARY INSTITUTE ON-GOING STUDIES ON VTEC IN SWEDEN Anna Aspán & friends

2 Chase-Topping M, Gally D, Low C, Matthews L, Woolhouse M. Nat Rev Microbiol Dec;6(12):

3 Green area routine testing in hospitals for VTEC Red area no routine testing in hospitals for VTEC Public Health Agency of Sweden

4 Infektion med VTEC / STEC Draft

5

6

7 Scotland Sweden Was it something we didn t do? Was it something that we did? Or doesn t it matter anyway?

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9 11% 33 % 13% 28%

10 Some of the knowledge gaps: Are there differences in virulence between different variants of VTEC O157:H7? Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population?

11 Some of the knowledge gaps: Are there differences in virulence between different variants of VTEC O157:H7? Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population? November 2014 Volume 52 Number 11 Journal of Clinical Microbiology p

12 Some of the knowledge gaps: Are there differences in virulence between different variants of VTEC O157:H7? Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population?

13 Some of the knowledge gaps: Are there differences in virulence between different variants of VTEC O157:H7? Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population?

14 q-pcr screening of faecal & ear samples, slaughterhouse prevalence study, (n=2580) ca q-pcr reactions, most in duplex format

15 Positive samples (%) 90,0 80,0 Proportion of positive samples 95% CI 70,0 60,0 50,0 40,0 30,0 20,0 10,0 0,0 O145 O103 O104 O157 O121 O26 SF-p VT1 VT2 VT2a Ears Fecal samples

16 O157 H7 O26 H O103 H2 O145 H O121 H

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19 Matrix No. of PCR+ samples PCR+ samples with Ct <25 No. (%) with VTEC isolation PCR+ samples with Ct > 25 No. of PCR+ samples No. (%) with VTEC isolation PCR+ samples Without Ct No. of PCRsamples No. (%) with VTEC isolation Serogroup of the isolated strains Cattle faeces Slaughterhouse prev study vtx + 3 vtx vtx + 11 vtx vtx + 11 vtx - VTEC O26 Cattle ear samples Slaughterhouse prev study vtx + 1 vtx vtx + 15 vtx vtx + 8 vtx - VTEC O26

20 q-pcr pos samples (n=23) 5 vtx1 +intimin 1 vtx1 -intimin 2 vtx2 +intimin 12 vtx- +intimin 3 vtx- -intimin q-pcr pos samples (n=20) 4 vtx1 +intimin 13 vtx- +intimin 3 vtx- -intimin q-pcr neg samples (n=14) 3 vtx1 +intimin 11 vtx- +intimin q-pcr neg samples (n=16) 7 vtx1 +intimin 1 vtx1+2 +intimin 8 vtx- +intimin

21 Longitudinal study of VTEC O157:H7 in dairy cattle herds in two areas in a high prevalence region Some of the knowledge gaps: Are there differences in virulence between different variants of VTEC O157:H7? Tamminen et al., (in manuscript) Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population?

22 Longitudinal study over 38 months of the verotoxigenic Escherichia coli O157:H7 status in 126 cattle herds Widgren, Söderlund, Fast, Eriksson, Aspán, Emanuelson, Alenius, Lindberg (in manuscript)

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24 Canadian vaccin towards O157:H7 20 farms with high recovery of VTEC O157 in cattle in the island of Öland 10 frams vaccination 10 farms as controls Some of the knowledge gaps: Vaccination of young calves, i.e. animals that are most often positive for VTEC O157 ½ dose of the vaccin for animals < 6 weeks On the two first farms three claves died when vaccinated Are there differences in virulence between different variants of VTEC O157:H7? Can we find better (and cheaper) ways of sampling for O157:H7 and other relevant serotypes of VTEC? Can we find better (and cheaper) ways of isolating O157:H7 and other relevant serotypes of VTEC? The trial had to be abandonen Can we learn more about the epidemiology of VTECs within and between cattle herds? Is it possible to lower the excreation rate of VTEC O157:H7 in the cattle population?

25

26 WGS implementation comes close to rivaling PFGE in terms of speed and cost while producing far more typing data than both PFGE and MLVA.

27 Robert Söderlund Stefan Widgren Christine Källman Tomas Jinnerot Erik Eriksson Ann Lindberg Stefan Alenius Ulf Emanuelsson Lena Maria Tamminen Madde Tråvén Cecilia Jernberg Ingela Hedenström Cecilia Svensson Camilla Sundborger Sofie Ivarsson

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