A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus

Size: px
Start display at page:

Download "A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus"

Transcription

1 197 Appendix 24 A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus N.P. Ferris a, A.N. Bulut b, T. Rendle a, F. Davidson a and D.K.J. Mackay c a b c Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK FMD Institute, PK 714, Ulus, Ankara, Turkey Veterinary Medicines Directorate, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK Summary A solid-phase competition ELISA (SPCE) has been devised for the measurement of antibodies to foot-and-mouth disease (FMD) virus. The assay uses polyclonal antisera and inactivated purified 146S antigens of FMD virus and was compared with the liquid-phase blocking ELISA (LPBE) and virus neutralisation test (VNT) on a range of serum sets. The SPCE exhibited equal test sensitivity as the LPBE and VNT and a better specificity of reaction than the LPBE when using test sera at a 1:5 dilution and a cut-off point of 30 percentage inhibition of antigen. The assay thus retains the sensitivity and ease of use of the LPBE whilst being more robust and specific and offers an improvement for FMD virus antibody detection. Introduction The OIE prescribed tests for foot-and-mouth disease (FMD) serology are the virus neutralisation test (VNT; Golding et al., 1976) and the liquid-phase blocking ELISA (LPBE; Hamblin et al., 1986). The LPBE has since been adopted by a large number of laboratories worldwide (Mackay et al., 1994, 1998) to replace the VNT for routine screening because it is quicker, more reproducible, correlates well with the VNT and does not suffer from the biological variability that is inherent in the VNT. However, the test is not without its problems. The percentage of animals giving false-positive results varies according to the animal population particularly in animals which are stressed and can be as high as 4% (Haas, 1994). For this reason, it is usually recommended that low-positive LPBE results are confirmed by VNT, particularly for the purposes of international trade (Anon, 1996). The assay requires training and experience to yield reproducible results and is not particularly 'robust'. A major factor for the lack of robustness of the LPBE has been in the variable stability of inactivated antigens employed in the procedure. We decided that it would be worthwhile to examine a solid-phase competition ELISA (SPCE) for FMD virus serology using polyclonal antiserum reagents (as used by the LPBE)

2 198 and inactivated, purified 146S antigens of FMD virus which have been observed to be remain stable after long term (years) storage at 4 o C (N.P. Ferris, unpublished results) Materials and methodsmaterials and methodsmaterials and methodsmaterials and methods Viruses FMD virus types O 1 BFS 1860, A 22 IRQ 24/64 and C 1 Noville were grown on monolayers of BHK-21 cells, inactivated with 1 M binary ethyleneimine (BEI; Bahnemann, 1990), 146S antigens purified according to the method of Ferris et al., 1984 and stored at 4 o C for subsequent use in the SPCE. Test Serum Samples Four sets of positive sera were examined : i) reference sera obtained from cattle between 21 and 28 days after either infection or vaccination with single, defined strains of FMD virus of known origin; ii) sera collected sequentially following either infection (type O 1 BFS) or vaccination (types A 22 and C 1 ) with FMD virus; iii) sera generated for the FAO Collaborative Studies Phases XIII (Mackay et al., 1994) and XV (Mackay et al., 1998) and iv) 'problem' sera : sera from cattle or water buffaloes which had been found to be low positive by LPBE and/or VNT on serological testing of animals prior to international trade but in which there was no recorded history of previous vaccination or infection. Additionally, negative sera from the UK, Canada or elsewhere which originated from animals with no history of exposure to FMD virus and which had been tested by LPBE and/or VNT with negative results. Virus neutralisation test and Liquid-phase blocking ELISA (LPBE) Virus neutralisation tests (VNT) were performed in tissue culture grade microtitre plates using the method described by Golding et al. (1976). The LPBE was carried out as described by Hamblin et al. (1986). Solid-phase competition ELISA (SPCE) The final procedure adopted for the solid-phase competition ELISA (SPCE) was as follows (50 µl reagent volumes were used throughout, ELISA plates [Nunc Maxisorp immunoplates] incubated for 1 hour in 37 o C on a rotary shaker, unless otherwise stated, and plates washed three times with phosphate buffered saline [PBS, ph 7.4] containing 0.05% Tween 20 after each incubation step). Plates were coated with an optimal dilution of rabbit antiserum to FMD virus in carbonate/bicarbonate buffer, ph 9.6 and incubated overnight at +4 o C. Next an optimal dilution of 146S antigen of FMD virus homologous to

3 199 the rabbit antiserum was diluted in PBS containing 0.05% Tween 20 and phenol red indicator (PBST) and added to each well. Duplicate, two-fold dilutions of each test serum (from an initial dilution of 1:2.5) in PBST containing 10% normal serum of the species under test and 5% normal rabbit serum (blocking buffer 3) were performed in plates. Immediately homologous guinea pig antiserum, diluted to the optimal concentration in blocking buffer, was added to each well. After plate incubation, an optimal dilution of rabbit anti-guinea pig immunoglobulins conjugated to horse radish peroxidase in blocking buffer was added to each well. Substrate (0.05% H 2 O 2 )/chromogen (orthophenylene diamine) in citrate/phosphate buffer, ph 5.0 was next added. After 15 min incubation the reaction was stopped by adding 1.25 M sulphuric acid. The optical density (OD) of each well was read by using a spectrophotometer (Dynatech) with a 492 nm filter. Antibody titres were expressed as the last dilution of serum showing 30% inhibition of OD compared to the mean OD of the reaction control wells where serum was absent. Blocking Buffers Three different blocking buffers were evaluated using defined positive and negative sera: i) blocking buffer 1 - PBS with 0.05% Tween 20 containing 5% skimmed milk powder ("Marvel"); ii) blocking buffer 2 - PBS (ph 7.4) with 0.1% Tween 20 and containing 0.3% normal serum of the species under test and iii) blocking buffer 3 PBS (ph 7.4) with 0.05% Tween 20 and containing 10% normal serum of the species under test and 5% normal rabbit serum. Results Differentiation of positive from negative sera To evaluate the performance of the test, 57 positive reference sera for type O and 120 negative sera were titrated in the SPCE for antibody to FMD virus type O 1 BFS 1860 in a dilution series from 1:2.5 to 1:80. The results were analysed in terms of percentage inhibition of antigen at each serum dilution (Fig. 1). The dilution at which there was optimal differentiation of positive from negative sera was 1:5. All positive sera had more than 30% inhibition and all negative sera less than this value. At this dilution there was no overlap in the two populations using blocking buffer 3, in contrast to considerable overlap of the positive and negative populations using blocking buffers 2 and 3 (results not shown). Blocking buffer 3 was therefore used for all subsequent work. Having established a provisional cut-off for the test, large numbers of negative sera were then examined at a single dilution of 1:5 (753 sera against type O 1 and 216 against types A 22 and C, of which 113 sera were examined against all 3 serotypes). Frequency distributions showed the percentage inhibitions of the negative sera to be normally distributed (Fig. 2). For a normal population, establishing a cut-off at the mean + 3

4 200 standard deviations (SD's) should mean that less than 1% of the values exceed the cut-off. For the negative populations studied here, the mean % inhibitions for types O, A and C were 9, 10 and 10% respectively with standard deviations of 6% in each case. This gave cut-off values of 27, 28 and 28% for types O, A and C respectively. To simplify interpretation and to ensure a high level of specificity, the cut-off was therefore set at 30% for all serotypes. Using this cut-off, the specificity of the assays for types A and C was 100% (zero positive out of 216). For type O, the specificity was 99.3% as 5 out of 753 sera scored positive at the single dilution of 1:5. However, all 5 sera were negative in a titration ELISA (i.e. titre <1:5) raising the specificity of the overall testing procedure to 100% for this population. Correlation with LPBE End point titres were determined in the SPCE and LPBE for positive reference sera for each of the serotypes O, A and C (Fig. 3). Statistically significant correlations were measured for each serotype and all sera positive by LPBE were also positive by SPCE. This result also shows that the SPCE detects antibody to a wide variety of strains within each serotype. For each serotype a single strain of FMD virus was used as the antigen in ELISA, whereas a wide variety of strains from within each serotype was used for infection or vaccination of cattle to produce the reference sera. The dynamics of the antibody responses to infection (type O) and vaccination (types A and C) showed that antibody titres were similar irrespective of measurement by either the LPBE or SPCE (results not shown). Titres in the low positive/doubtful range were measured at 7 days post vaccination (dpv) and all animals were strongly seropositive by 14 dpv. The sensitivity and reproducibility of the SPCE were compared directly with the LPBE and the VNT. Five two-fold dilutions of a type O reference serum were prepared in normal negative bovine serum such that the estimated end point titre in the SPCE was "bracketed" in either direction. These sera were then randomly labelled from A to E and repeatedly examined blind by SPCE, LPBE and by VNT, together with the original positive reference serum (N; Table 1). The SPCE and the LPBE had the same sensitivity, both scoring the 1:160 dilution (B) positive and the 1:320 dilution (D) negative. The VNT was slightly less sensitive than either ELISA scoring the 1:160 dilution (B) doubtful (i.e. titre between 1:16 and 1:32) but the 1:80 dilution (E) positive (i.e. titre >1:45). In terms of reproducibility, both ELISA's generally had lower coefficients of variation (CV) than the VNT. The CV's of the sera in the SPCE were similar to but slightly higher than those in the LPBE. Sera from two FAO Standardisation Exercises were examined by SPCE, LPBE and the VNT. In the Phase XIII Exercise (Table 2), panels of four reference sera for each of the strains O 1 BFS 1860, A 22 IRQ 24/64 and C 1 Noville were created which varied in strength

5 201 from weak positive through to strong positive. Although the absolute values varied between assays, there was good correlation between the three assays. All the reference sera correctly scored positive in the SPCE with the exception of RS-4 for type O 1 BFS 1860 which was negative by half a dilution step (titre 1:3.5; cut-off 1:5). However, this serum was also doubtful by VNT with a titre of 1:32 (cut-off in VNT 1:45).There was also good correlation between the results of all three tests in examination of the Phase XV provisional reference sera (results not shown). Using as antigen either the homologous strain of FMD virus with which the animal was infected or a heterologous strain of the same or a different serotype, there was a high degree of cross-reactivity between the two strains of type O in each of the 3 assays but the extent of cross-reactivity was greatest in the SPCE (results not shown). Some degree of cross-reactivity between serotypes was observed in all three assays for all three serotypes. In all cases, there was less cross-reactivity between serotypes in the SPCE than in the LPBE or VNT, suggesting that the SPCE is more serotype specific than the other two tests. Of the 73 of 85 >doubtful sera= which were positive in the LPBE for antibody to type O, only 3 were positive in the SPCE, one of which was also positive by VNT (Table 3). Likewise for antibody to type A, only 4 out of 48 sera which were LPBE positive were also positive by SPCE and two of these were also VNT positive. Assuming that these sera were actually from FMD naive animals, which could not be proven beyond doubt, then the SPCE was considerably more specific than the LPBE for these 'doubtful' sera and was only slightly less specific than the definitive VNT. Discussion Analysing the results overall, higher concentrations of positive sera were required to achieve a given percentage inhibition in the SPCE than in the LPBE. This meant that the cut-off in the SPCE was at a lower dilution (1:5) than in the LPBE (1:40; Hamblin et al., 1986) and at a lower percentage inhibition (30% as opposed to 50%). Nevertheless, using this cut-off value, the sensitivities of the two ELISA's were almost identical (Table 1). Only some sera with the lowest positive titre by LPBE (1:45) just scored negative (1:3.5) in the SPCE. The SPCE was at least as sensitive (Table 1) and certainly more specific (Table 3) than the 'gold standard' VNT. Any new test for antibody to FMDV must score correctly reference sera which are recognised as international standards. The SPCE scored the provisional FAO reference sera for FMD virus types O 1 Manisa, A 22 IRQ 24/64 and C 1 Noville correctly (Table 2) and showed good correlation with both the LPBE and the VNT for panels of positive reference sera to FMD virus type O 1 BFS 1860, A 22 IRQ 24/64 and C 1 Noville. When using serology for diagnosis of FMD it is useful to have a test which is as broadly cross-reactive between strains of a given serotype, but as serotype-specific as possible. Examining selected

6 202 reference sera by SPCE, LPBE and VNT showed that the SPCE most closely matched these criteria. Although positive titres were recorded against serotypes other than the one used to infect the animal, their heterologous titres were universally low, and lower in comparison, than those recorded in the LPBE and the VNT. The ability of the SPCE to detect antibody generated by exposure to a wide variety of strains within a serotype is best shown in Fig 3. For all 3 serotypes, all reference sera collected between 21 and 28 days after either infection or vaccination with any strain within a serotype gave a positive reaction in the SPCE using as antigen purified FMD virus of the homologous serotype. This figure also shows a strong correlation between titres in the SPCE and in the LPBE (and the r values would have been higher if end point titres had been determined for all sera having an SPCE titre of greater than 1:160). The fact that some sera had a high titre by LPBE, but a low titre by SPCE and vice versa suggests that the population of antibodies measured in the two tests were not identical. This is to be expected as the LPBE measures both antibody which prevents binding of the antigen in the liquid phase to the immobilised rabbit antiserum and antibody which blocks the detecting guinea pig antiserum. In contrast, the SPCE relies only on competition between the test serum and the detecting guinea pig antiserum. The objective of the work reported here was to develop an ELISA for antibody to FMD virus which retained the sensitivity and ease of use of the LPBE whilst being more robust and, hopefully, more specific. A sensitive, specific and reliable SPCE has been developed to measure antibody to FMD virus to fulfill the objective. Work is now in hand to adopt the assay for use with the other FMD virus serotypes and to evaluate the test in day-to-day use in the WRL for FMD. A full presentation of this work is in preparation for submission to the Journal of Virological Methods. Acknowledgements This work was supported financially by the UK Ministry of Agriculture, Fisheries and Food (project number SE 1113). The authors wish to thank members of the International Vaccine Bank, Pirbright Laboratory and Dr J. Salt for provision of test sera. ReferencesReferencesReferencesReferences Anon. (1996). OIE Manual of Standards for Diagnostic Tests and Vaccines. Lists A and B diseases of mammals, birds and bees. Chapter Foot and Mouth Disease, pp Bahnemann, H.G. (1990). Vaccine 8, Ferris, N.P., Donaldson, A.I., Barnett, I.T.R. and Osborne, R.W. (1984). Revue Scientific et Technique de l'office International des Epizooties 3, Golding, S.M., Hedger, R.S. and Talbot, P. (1976). Research in Veterinary Science 20, 142-

7 Haas, B. (1994). Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Vienna, September, 1994, Hamblin, C., Barnett, I.T.R. and Hedger, R.S. (1986). Journal of Immunological Methods 93, Mackay, D.K.J., Rendle, T. and Armstrong, R.M. (1994). Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Vienna, September, 1994, Mackay, D.K.J., Rendle, T., and Kitching, R.P. (1998). Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Aldershot, UK, September, 1998, Mackay, D.K.J., Bulut, A.N., Rendle, T., Davidson, F. and Ferris, N.P. In preparation for submission to the Journal of Virological Methods. Table 1 Two-fold dilutions (shown in brackets) of a positive serum (N) diluted in normal bovine serum and repeatedly tested by SPCE, LPBE and VNT. Test Sera C (1280) A (640) D (320) B (160) E (80) N (neat, undiluted serum) SPCE a b 0% c 0% % % % % LPBE % % % % % % a b c VNT % % % % % % Mean titre (n=5 for SPCE and LPBE; n=3 for VNT) of each serum as logarithmic value Standard deviation (SD) Co-efficient of variation Number in parenthesis shows the serum dilution

8 204 Table 2 Examination of the provisional reference sera from the FAO Phase XIII Standardisation Exercise by SPCE, LPBE and VNT Phase XIII O 1 BFS 1860 RS-1 RS-2 RS-3 RS-4 SPCE LPBE VNT 452 a b Phase XIII A 22 IRQ 24/64 RS-1 RS-2 RS-3 RS-4 SPCE LPBE VNT Phase XIII C 1 Noville RS-1 RS-2 RS-3 RS-4 a b SPCE LPBE VNT Left hand columns : arithmetic titre Right hand columns : logarithmic titre Table 3 A panel of 85 >problem= sera were selected for examination by SPCE, LPBE and VNT on the basis of previous reactivity in the LPBE for import/export testing and assumed to originate from FMD virus naive cattle FMD virus Test result Test SPCE LPBE VNT O 1 BFS 1860 Number positive Number negative Specificity (%) A 22 IRQ 24/64 Number positive Number negative Specificity (%)

9 205

FAO Collaborative Study Phase XVII: Standardisation of FMD Antibody Detection

FAO Collaborative Study Phase XVII: Standardisation of FMD Antibody Detection Appendix 28 FAO Collaborative Study Phase XVII: Standardisation of FMD Antibody Detection D J Paton, R M Armstrong, L S Turner, P A Hamblin, M Corteyn, D Gibson, J Anderson Institute for Animal Health,

More information

COMPARISON OF DIFFERENT ELISA METHODS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS (FMDV) TYPE O

COMPARISON OF DIFFERENT ELISA METHODS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS (FMDV) TYPE O Bull. Vet. Inst. Pulawy 48, 5-9, 24 COMPARISON OF DIFFERENT ELISA METHODS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS (FMDV) TYPE O WIESŁAW NIEDBALSKI Department of Foot-and-Mouth

More information

Appendix 71 Secretory IgA as an indicator of oropharyngeal FMDV replication Abstract Introduction Materials and methods

Appendix 71 Secretory IgA as an indicator of oropharyngeal FMDV replication Abstract Introduction Materials and methods Appendix 71 Secretory IgA as an indicator of oropharyngeal FMDV replication Satya Parida, David Paton*, Sarah Cox, Paul Barnett, John Anderson Pirbright Laboratory, Ititute for Animal Health, Ash Road,

More information

Appendix 72 Using NSP ELISA (Chekit-FMD-3ABC Bommeli-Intervet) as a Tool for FMDV Serosurveillance in Bulgaria Abstract: Introduction

Appendix 72 Using NSP ELISA (Chekit-FMD-3ABC Bommeli-Intervet) as a Tool for FMDV Serosurveillance in Bulgaria Abstract: Introduction Appendix 72 Using NSP ELISA (Chekit-FMD-3ABC Bommeli-Intervet) as a Tool for FMDV Serosurveillance in Bulgaria Georgi Georgiev*¹, Emiliya Veleva¹, Liliyana Polihronova¹ and Alessandro Rossi² 1 National

More information

Regulation of FMD vaccines within the European Union

Regulation of FMD vaccines within the European Union Introduction Regulation of FMD vaccines within the European Union K De Clercq 1 and D K J Mackay 2 Appendix 36 The EUFMD European Pharmacopoeia Working Group made a proposal for revision of the FMD vaccine

More information

Longevity of the antibody response in pigs and sheep following a single administration of high potency emergency FMD vaccines

Longevity of the antibody response in pigs and sheep following a single administration of high potency emergency FMD vaccines 247 Appendix 31 Longevity of the antibody response in pigs and sheep following a single administration of high potency emergency FMD vaccines S. J Cox and P. V. Barnett Institute for Animal Health, Pirbright

More information

Bovine Brucellosis Control of indirect ELISA kits

Bovine Brucellosis Control of indirect ELISA kits Bovine Brucellosis Control of indirect ELISA kits (Individual serum samples & pooled serum samples) Standard Operating Procedure Control of Bovine brucellosis Serum ELISA kits SOP Page 1 / 7 02 February

More information

Materials and Methods

Materials and Methods Appendix 18 Repeated administration of maximum payload emergency vaccines made from inactivated purified antigen concentrates do not induce significant titres of antibodies against non-structural proteins

More information

Appendix 30. Preliminary results to evaluate cross-protection between O 1 Manisa and O 1 Campos in cattle

Appendix 30. Preliminary results to evaluate cross-protection between O 1 Manisa and O 1 Campos in cattle Appendix 30 Preliminary results to evaluate cross-protection between O 1 Manisa and O 1 Campos in cattle V.A. Srinivasan 1, S.B.Nagendra Kumar 1, M.Madhan Mohan 1, V.Maroudam 1, P.Santha Kumar 1, S. Parida

More information

Manufacturers expected contribution to the progressive control of Foot-and-Mouth Disease in South Asia

Manufacturers expected contribution to the progressive control of Foot-and-Mouth Disease in South Asia Manufacturers expected contribution to the progressive control of Foot-and-Mouth Disease in South Asia Ph. Dubourget & al. 1 GENERAL PRINCIPLES 2 EPIDEMIO- LOGICALLY RELEVANT STRAINS 3 IMMUNO- DOMINANT

More information

Cedivac-FMD; Duration of Immunity in cattle, sheep and pigs. 2004, 8203 AA Lelystad, The Netherlands * Corresponding Author

Cedivac-FMD; Duration of Immunity in cattle, sheep and pigs. 2004, 8203 AA Lelystad, The Netherlands * Corresponding Author Appendix 3 Cedivac-FMD; Duration of Immunity in cattle, sheep and pigs. Paulus Selman *, Gilles Chénard and Aldo Dekker Animal Sciences Group, Wageningen UR, P.O. Box 65, 8 AB Lelystad, The Netherlands

More information

Field study conducted in Tunisia to evaluate efficacy of an O-BFS vaccine

Field study conducted in Tunisia to evaluate efficacy of an O-BFS vaccine Field study conducted in Tunisia to evaluate efficacy of an O-BFS vaccine FAO/OIE Reference Lab for FMD Emiliana Brocchi Institut de la Recherche Vétérinaire de Tunisie - Service Virologie Soufien Sghaier

More information

FMD Vaccine Strain Selection

FMD Vaccine Strain Selection FMD Vaccine Strain Selection David Paton, Pirbright, UK New Delhi, 13-15 February 2012 Conclusions Vaccine match is one component of vaccine efficacy Vaccine quality may compensate for imperfect match

More information

FOOT AND MOUTH DISEASE DIAGNOSTICS: REQUIREMENTS FOR DEMONSTRATION OF FREEDOM FROM INFECTION

FOOT AND MOUTH DISEASE DIAGNOSTICS: REQUIREMENTS FOR DEMONSTRATION OF FREEDOM FROM INFECTION 70 SG/12/CS3 C Original: English FOOT AND MOUTH DISEASE DIAGNOSTICS: REQUIREMENTS FOR DEMONSTRATION OF FREEDOM FROM INFECTION R.P. Kitching Director, Canadian Food Inspection Agency, National Centre for

More information

Bordetella pertussis igg-pt elisa Kit

Bordetella pertussis igg-pt elisa Kit Bordetella pertussis IgG-PT ELISA Kit Application Bordetella pertussis IgG-PT ELISA Kit is a quantitative test for the detection of IgG antibodies against pertussis toxin in human serum samples. Background

More information

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit 2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit Catalog Number : SEK001 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as

More information

High potency vaccines induce protection against heterologous challenge with FMD virus

High potency vaccines induce protection against heterologous challenge with FMD virus High potency vaccines induce protection against heterologous challenge with FMD virus Katharina Brehm, Naveen Kumar, Hans-Herman Thulke and Bernd Haas Principle of current production method for FMD vaccines

More information

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum Please read carefully due to Critical Changes, e.g., see Calculation of Results. For Research Use Only. Not For Use In Diagnostic

More information

Validation report. First commercially-available ELISA which allows for the detection of Lumpy Skin Disease antibodies

Validation report. First commercially-available ELISA which allows for the detection of Lumpy Skin Disease antibodies Validation report ID Screen Capripox Double Antigen Multi-species Multi-species Double Antigen ELISA for detection of antibodies against capripoxviruses including Lumpy skin disease virus (LSDV), sheeppox

More information

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK001 To achieve the best assay results, this manual must be read carefully before using this product and the assay

More information

Overview of WRL FMD. Historical perspective. Principal activities. FMD threats. Needs/prospects. David Paton

Overview of WRL FMD. Historical perspective. Principal activities. FMD threats. Needs/prospects. David Paton Tracking the emergence and global spread of foot-and-mouth disease (FMD) Overview of WRL FMD David Paton Historical perspective Principal activities FMD threats Needs/prospects Historical Perspective FMD

More information

Vaccine matching. Reliability of foot-and-mouth disease r-values. determination

Vaccine matching. Reliability of foot-and-mouth disease r-values. determination Vaccine matching Reliability of foot-and-mouth disease r-values determination Interinstitutional Network for R&D in FMD (RIIDFA)- Argentina Veterinary and Agrochemical Research Centre (VAR) - Belgium Protection

More information

Influenza A H1N1 HA ELISA Pair Set

Influenza A H1N1 HA ELISA Pair Set Influenza A H1N1 HA ELISA Pair Set for H1N1 ( A/Puerto Rico/8/1934 ) HA Catalog Number : SEK11684 To achieve the best assay results, this manual must be read carefully before using this product and the

More information

AFLATOXIN M1 CAT. NO. 961AFLMO1M

AFLATOXIN M1 CAT. NO. 961AFLMO1M h AFLATOXIN M1 CAT. NO. 961AFLMO1M Competitive ELISA Immunoassay for the quantitative detection of Aflatoxin M1 in milk, milk powder and cheese. General Aflatoxins are toxic metabolites produced by a variety

More information

Herpes Simplex Virus 2 IgM HSV 2 IgM

Herpes Simplex Virus 2 IgM HSV 2 IgM DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and 116, Woodland Hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com

More information

Reagents for the Typing of Human Influenza Isolates 2011

Reagents for the Typing of Human Influenza Isolates 2011 Reagents for the Typing of Human Influenza Isolates 2011 This product was developed by the Victorian Infectious Diseases Reference Laboratory (VIDRL) in its capacity as a WHO Collaborating Centre for Reference

More information

Insulin (Porcine/Canine) ELISA

Insulin (Porcine/Canine) ELISA Insulin (Porcine/Canine) ELISA For the quantitative measurement of insulin in Porcine/Canine serum and plasma (EDTA) For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSPO-E01

More information

See external label 96 tests HSV 2 IgA. Cat #

See external label 96 tests HSV 2 IgA. Cat # DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

Abraxis Progesterone (bovine) ELISA Kit

Abraxis Progesterone (bovine) ELISA Kit Abraxis Progesterone (bovine) ELISA Kit Enzyme immunoassay for the quantitative determination of progesterone in bovine milk/serum/plasma samples PN5081M 96 Tests For Research Use Only. Not for use in

More information

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus J. gen. Virol. (198o), 50, 369-375 Printed #~ Great Britain 369 Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus By B. CARTWRIGHT, W. G. CHAPMAN

More information

Comparison of protective Antibody response in guinea pigs immunized with Wild type (Wt) BoHV-1 and ΔgE BoHV-1 mutant virus vaccine

Comparison of protective Antibody response in guinea pigs immunized with Wild type (Wt) BoHV-1 and ΔgE BoHV-1 mutant virus vaccine Comparison of protective Antibody response in guinea pigs immunized with Wild type (Wt) BoHV-1 and ΔgE BoHV-1 mutant virus vaccine L.R.ANANTHAKRISHNA 1*, MOHINI SAINI 2, PRAVEEN K GUPTA 3 and RIZWANA TABASSUM

More information

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual)

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) BACKGROUND Human Immunodeficiency Virus ( HIV ) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to

More information

Bovine Insulin ELISA

Bovine Insulin ELISA Bovine Insulin ELISA For quantitative determination of insulin in bovine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSBO-E01 Size: 96 wells Version:

More information

G. W. WOOD J. C. MUSKETT and D. H. THORNTON MAFF, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, U.K.

G. W. WOOD J. C. MUSKETT and D. H. THORNTON MAFF, Central Veterinary Laboratory, New Haw, Weybridge, Surrey, U.K. J. Comp. Path. 1986 vol. 96 OBSERVATIONS ON THE ABILITY OF AVIAN REOVIRUS VACCINMATION OF HENS TO PROTECT THEIR PROGENY AGAINST THE EFFECTS OF CHALLENGE WITH HOMOLOGOUS AND HETEROLOGOUS STRAINS By G. W.

More information

DIAGNOSTIC AUTOMATION, INC.

DIAGNOSTIC AUTOMATION, INC. DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set

Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK40103 To achieve the best assay results, this manual must be read carefully before using this product and the assay

More information

Rat Insulin ELISA. For the quantitative determination of insulin in rat serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.

Rat Insulin ELISA. For the quantitative determination of insulin in rat serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Rat Insulin ELISA For the quantitative determination of insulin in rat serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-INSRT-E01, E10 96 wells, 10

More information

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures.

Rat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures. Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-CPTRT-E01 96 wells Version: May 26,

More information

COMMITTEE FOR MEDICINAL PRODUCTS FOR VETERINARY USE

COMMITTEE FOR MEDICINAL PRODUCTS FOR VETERINARY USE European Medicines Agency Veterinary Medicines and Inspections EMEA/CVMP/775/02-FINAL COMMITTEE FOR MEDICINAL PRODUCTS FOR VETERINARY USE POSITION PAPER ON REQUIREMENTS FOR VACCINES AGAINST FOOT-AND-MOUTH

More information

Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines

Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines Final Report APL Project 211/139.45 December 211 Australian Animal Health Laboratory Dr Wilna Vosloo Private Bag 24 Geelong Vic

More information

Human Obestatin ELISA

Human Obestatin ELISA K-ASSAY Human Obestatin ELISA For the quantitative determination of obestatin in human serum and plasma Cat. No. KT-495 For Research Use Only. 1 Rev. 081309 K-ASSAY PRODUCT INFORMATION Human Obestatin

More information

Human Apolipoprotein A1 EIA Kit

Human Apolipoprotein A1 EIA Kit A helping hand for your research Product Manual Human Apolipoprotein A1 EIA Kit Catalog Number: 83901 96 assays 1 Table of Content Product Description 3 Assay Principle 3 Kit Components 3 Storage 4 Reagent

More information

This CRP is proposed for five years with three RCM. To apply, please see our website for directions:

This CRP is proposed for five years with three RCM. To apply, please see our website for directions: 1. CRP on the control of foot-and-mouth disease 2. Summary Foot-and-mouth disease (FMD) is one of the most important livestock diseases known to man due to its high infection rate (ease of spread) and

More information

ELEGANCE Chlamydia pneumoniae IgG ELISA KIT

ELEGANCE Chlamydia pneumoniae IgG ELISA KIT INTENDED USE The ELEGANCE Chlamydia pneumoniae IgG ELISA has been designed for the in vitro diagnostic measurement of anti- Chlamydia pneumoniae IgG in the screening of human serum. PRINCIPLES OF THE ELEGANCE

More information

Mouse C-peptide ELISA

Mouse C-peptide ELISA Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calculation

More information

REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIA DEPARTMENT OF LIVESTOCK DEVELOPMENT PAKCHONG, NAKHONRATCHASIMA, THAILAND

REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIA DEPARTMENT OF LIVESTOCK DEVELOPMENT PAKCHONG, NAKHONRATCHASIMA, THAILAND University of Tokyo, Tokyo, Japan, 9-11 June 2015 REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIA DEPARTMENT OF LIVESTOCK DEVELOPMENT PAKCHONG, NAKHONRATCHASIMA, THAILAND Activities of FMD Laboratory

More information

Cotinine (Mouse/Rat) ELISA Kit

Cotinine (Mouse/Rat) ELISA Kit Cotinine (Mouse/Rat) ELISA Kit Catalog Number KA2264 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

WHO Prequalification of In Vitro Diagnostics Programme PUBLIC REPORT. Product: Murex HIV Ag/Ab Combination Number: PQDx

WHO Prequalification of In Vitro Diagnostics Programme PUBLIC REPORT. Product: Murex HIV Ag/Ab Combination Number: PQDx WHO Prequalification of In Vitro Diagnostics Programme PUBLIC REPORT Product: Murex HIV Ag/Ab Combination Number: PQDx 0144-043-00 Abstract Murex HIV Ag/Ab Combination with product codes 7G79-09 (GE41,

More information

Porcine/Canine Insulin ELISA

Porcine/Canine Insulin ELISA Porcine/Canine Insulin ELISA For the quantitative determination of insulin in porcine or canine serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research

More information

Mechanism of FMD spread in East Asia

Mechanism of FMD spread in East Asia Mechanism of FMD spread in East Asia China has the important role of the FMD outbreaks in East Asia 1) Long border with FMD epidemic countries in South East and Central Asia 2) Large numbers of susceptible

More information

Instructions for Use. Tg Antibody ELISA

Instructions for Use. Tg Antibody ELISA Instructions for Use Tg Antibody ELISA Enzyme Immunoassay for the Quantitative Determination of Autoantibodies to Thyroglobulin (Tg) in Serum and Plasma I V D REF EA618/96 12 x 8 2 8 C DLD Gesellschaft

More information

FOOT AND MOUTH DISEASE

FOOT AND MOUTH DISEASE NB: Version adopted by the World Assembly of Delegates of the OIE in May 2012 CHAPTER 2.1.5. FOOT AND MOUTH DISEASE SUMMARY Foot and mouth disease (FMD) is the most contagious disease of mammals and has

More information

This product was developed by the Victorian Infectious Diseases Reference Laboratory (VIDRL) in its capacity as a WHO Collaborating Centre for

This product was developed by the Victorian Infectious Diseases Reference Laboratory (VIDRL) in its capacity as a WHO Collaborating Centre for This product was developed by the Victorian Infectious Diseases Reference Laboratory (VIDRL) in its capacity as a WHO Collaborating Centre for Reference and Research on Influenza, with material provided

More information

LABORATORY CONTINGENCY PLAN MANUAL FOR FMD (INTERNET VERSION)

LABORATORY CONTINGENCY PLAN MANUAL FOR FMD (INTERNET VERSION) DRAFT 10 LABORATORY CONTINGENCY PLAN MANUAL FOR FMD (INTERNET VERSION) Institute for Animal Health Pirbright Laboratory Edition Number Authorised By Issue Date Review Date 9 Dr D. Paton 21 st July 2006

More information

Herpes Simplex Virus 2 IgG HSV 2 IgG

Herpes Simplex Virus 2 IgG HSV 2 IgG DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and 116, Woodland Hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com

More information

Mouse Ultrasensitive Insulin ELISA

Mouse Ultrasensitive Insulin ELISA Mouse Ultrasensitive Insulin ELISA For the quantitative determination of insulin in mouse serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research Use

More information

Cereal yellow dwarf virus - RPV ELISA Kit

Cereal yellow dwarf virus - RPV ELISA Kit Cereal yellow dwarf virus - RPV ELISA Kit Cat. No.:DEIA9232 Pkg.Size:96T Intended use The Cereal yellow dwarf virus - RPV ELISA Kit is a qualitative serological assay for the detection of CYDV-RPV in plant

More information

Trichinella Cat #

Trichinella Cat # DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

Rat Proinsulin ELISA

Rat Proinsulin ELISA Rat Proinsulin ELISA For the quantitative determination of proinsulin in rat serum For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-PINRT-E01 Size: 96 wells Version: June

More information

E. Histolytica IgG ELISA Kit

E. Histolytica IgG ELISA Kit E. Histolytica IgG ELISA Kit Catalog Number KA3193 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of

More information

Dissecting Immune Responses. Miriam Windsor, Nicholas Juleff, Mandy Corteyn, Pippa Hamblin, Veronica Carr, Paul V Barnett, Bryan Charleston*

Dissecting Immune Responses. Miriam Windsor, Nicholas Juleff, Mandy Corteyn, Pippa Hamblin, Veronica Carr, Paul V Barnett, Bryan Charleston* Appendix 28 Dissecting Immune Responses Miriam Windsor, Nicholas Juleff, Mandy Corteyn, Pippa Hamblin, Veronica Carr, Paul V Barnett, Bryan Charleston* Abstract: Pirbright Laboratory, Institute for Animal

More information

Longevity of protection in cattle following vaccination with emergency FMD vaccines from the UK strategic reserve

Longevity of protection in cattle following vaccination with emergency FMD vaccines from the UK strategic reserve Longevity of protection in cattle following vaccination with emergency FMD vaccines from the UK strategic reserve Sarah Cox, Satya Parida, Pip Hamblin, Bartek Bankowski Veronica Carr, David Paton and Paul

More information

Mouse C-peptide ELISA

Mouse C-peptide ELISA Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Preparation

More information

Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Introduction: Materials and methods:

Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Introduction: Materials and methods: Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Haixue Zheng, Fan Yang, Ye Jin, Jianhong Guo, Jijun He, Kaiqi Lian, Zixiang Zhu, Weijun Cao, Lvlv,

More information

IV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use.

IV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use. Definitions Instructions for use REF Catalogue number IV2-113E Use by English Invitron Glargine ELISA Kit For in-vitro diagnostic use Σ 96 LOT IVD Lot/Batch Code Storage temperature limitations In vitro

More information

1. Engineering Foot-and-Mouth Disease Viruses with Improved

1. Engineering Foot-and-Mouth Disease Viruses with Improved Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Haixue Zheng, Fan Yang, Ye Jin, Jianhong Guo, Jijun He, Lvlv, Xuepeng Cai, Xiangtao Liu, Hong

More information

E. Histolytica IgG (Amebiasis)

E. Histolytica IgG (Amebiasis) DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and 116, Woodland hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com

More information

A COMPETITIVE ELISA FOR THE DETECTION OF GROUP-SPECIFIC ANTIBODY TO EQUINE ENCEPHALOSIS VIRUS

A COMPETITIVE ELISA FOR THE DETECTION OF GROUP-SPECIFIC ANTIBODY TO EQUINE ENCEPHALOSIS VIRUS A COMPETITIVE ELISA FOR THE DETECTION OF GROUP-SPECIFIC ANTIBODY TO EQUINE ENCEPHALOSIS VIRUS J.E. Crafford 1, A.J. Guthrie 2, M Van Vuuren 1, P.P.C Mertens 3, J.N. Burroughs 3, P.G. Howell 2, C. A. Batten

More information

Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set

Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set Catalog Number : SEK11695 To achieve the best assay results, this manual must be read carefully before using this product

More information

Ph. Eur. Reference Standard - LEAFLET

Ph. Eur. Reference Standard - LEAFLET European Directorate for the Quality of Medicines & HealthCare European Pharmacopoeia (Ph. Eur.) 7, Allée Kastner CS 30026, F-67081 Strasbourg (France) Tel. +33 (0)3 88 41 20 35 Fax. + 33 (0)3 88 41 27

More information

REAGENTS FOR THE TYPING OF HUMAN INFLUENZA ISOLATES 2017

REAGENTS FOR THE TYPING OF HUMAN INFLUENZA ISOLATES 2017 REAGENTS FOR THE TYPING OF HUMAN INFLUENZA ISOLATES 2017 This product was developed by the Victorian Infectious Diseases Reference Laboratory (VIDRL) in its capacity as a WHO Collaborating Centre for Reference

More information

Elements of FMD post-vaccination monitoring programme / Éléments du programme de surveillance post-vaccination contre la fièvre aphteuse

Elements of FMD post-vaccination monitoring programme / Éléments du programme de surveillance post-vaccination contre la fièvre aphteuse Workshop: harmonization of the FMD vaccination strategy in the North Africa / Atelier : harmonisation de la stratégie de vaccination contre la fièvre aphteuse en Afrique du Nord 30-31 mars 2016 - Tunis

More information

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Catalog Number : SEK11233 To achieve the best assay results, this manual must be read carefully before using this product

More information

FMD Carrier state and role of carrier buffalo as source of transboundary spread in Southeast Asia and Eastern Asia Satya Parida

FMD Carrier state and role of carrier buffalo as source of transboundary spread in Southeast Asia and Eastern Asia Satya Parida FMD Carrier state and role of carrier buffalo as source of transboundary spread in Southeast Asia and Eastern Asia Satya Parida, Pirbright, UK Foot-and-mouth disease Highly contagious disease of all the

More information

LABORATORY TRAINING ON FOOT AND MOUTH DISEASE DIAGNOSIS (2-7 May, 2011) TRAINING MANUAL

LABORATORY TRAINING ON FOOT AND MOUTH DISEASE DIAGNOSIS (2-7 May, 2011) TRAINING MANUAL LABORATORY TRAINING ON FOOT AND MOUTH DISEASE DIAGNOSIS (2-7 May, 2011) TRAINING MANUAL SAARC REGIONAL LEADING DIAGNOSTIC LABORATORY ON FOOT AND MOUTH DISEASE PROJECT DIRECTORATE ON FOOT AND MOUTH DISEASE

More information

WEST EURASIA FMD LAB PHASE ACTIVITY PROPOSAL. A. Naci BULUT Head of the Diagnosis Department, Şap Institute

WEST EURASIA FMD LAB PHASE ACTIVITY PROPOSAL. A. Naci BULUT Head of the Diagnosis Department, Şap Institute WEST EURASIA FMD LAB NETWORK WELNET FMD- 2ND PHASE ACTIVITY PROPOSAL FAO/the EuFMD Commission, 39th General Session, 27/28 April 2011 Rome, Italy A. Naci BULUT Head of the Diagnosis Department, Şap Institute

More information

Mouse/Rat THYROXINE (T4) ELISA Catalog No (96 Tests)

Mouse/Rat THYROXINE (T4) ELISA Catalog No (96 Tests) For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Mouse/Rat Thryroxine Kit is intended for the detection of total T4 in mouse/rat serum or plasma. SUMMARY AND EXPLANATION

More information

Mouse GLP-2 EIA. Cat. No. KT-374. For the quantitative determination of GLP-2 in mouse serum or plasma. For Research Use Only. 1 Rev.

Mouse GLP-2 EIA. Cat. No. KT-374. For the quantitative determination of GLP-2 in mouse serum or plasma. For Research Use Only. 1 Rev. Mouse GLP-2 EIA For the quantitative determination of GLP-2 in mouse serum or plasma. Cat. No. KT-374 For Research Use Only. 1 Rev. 11357374 PRODUCT INFORMATION Mouse GLP-2 EIA Cat. No. KT-374 INTENDED

More information

Triiodothyronine (T3) ELISA

Triiodothyronine (T3) ELISA For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Triiodothyronine (T3) ELISA Kit is intended for the detection of total T3 in human serum or plasma. For research

More information

Myeloperoxidase (MPO) ELISA Stool, Urine KIT

Myeloperoxidase (MPO) ELISA Stool, Urine KIT Myeloperoxidase (MPO) ELISA Stool, Urine KIT Cat. No.:DEIA6211 Pkg.Size:96T Intended use The Myeloperoxidase Assay is intended for the quantitative determination of Myeloperoxidase in urine and stool,

More information

Requirements of the Terrestrial Code for FMD surveillance. Dr David Paton Dr Gideon Brückner

Requirements of the Terrestrial Code for FMD surveillance. Dr David Paton Dr Gideon Brückner Requirements of the Terrestrial Code for FMD surveillance Dr David Paton Dr Gideon Brückner 1 Surveillance Close observation Origin of the word: Early 19th century: from French, from sur- over + veiller

More information

Adopted by CVMP 10 March Date for coming into effect 1 July Revised draft guideline agreed by Immunologicals Working Party 22 June 2017

Adopted by CVMP 10 March Date for coming into effect 1 July Revised draft guideline agreed by Immunologicals Working Party 22 June 2017 1 2 3 7 September 2017 EMA/CVMP/IWP/105506/2007-Rev.1 Committee for medicinal products for veterinary use (CVMP) 4 5 6 7 Guideline on data requirements for multi-strain dossiers for inactivated vaccines

More information

Influenza B Hemagglutinin / HA ELISA Pair Set

Influenza B Hemagglutinin / HA ELISA Pair Set Influenza B Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK11053 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized

More information

Prothrombin (Human) ELISA Kit

Prothrombin (Human) ELISA Kit Prothrombin (Human) ELISA Kit Catalog Number KA0496 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General

More information

colorimetric sandwich ELISA kit datasheet

colorimetric sandwich ELISA kit datasheet colorimetric sandwich ELISA kit datasheet For the quantitative detection of human IL5 in serum, plasma, cell culture supernatants and urine. general information Catalogue Number Product Name Species cross-reactivity

More information

Reagent Set DAS ELISA, Alkaline phosphatase label SRA 22001, SRA 23203, SRA 27703, SRA & SRA ToRSV, ArMV, GFLV, AnFBV and PDV

Reagent Set DAS ELISA, Alkaline phosphatase label SRA 22001, SRA 23203, SRA 27703, SRA & SRA ToRSV, ArMV, GFLV, AnFBV and PDV List of contents Lot number Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate 0.150 ml 0.275 ml 0.525

More information

Potato leafroll virus (PLRV) Reagent Set DAS ELISA, Alkaline phosphatase label Catalog number: SRA List of contents

Potato leafroll virus (PLRV) Reagent Set DAS ELISA, Alkaline phosphatase label Catalog number: SRA List of contents Potato leafroll virus (PLRV) Reagent Set List of contents Lot number Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate

More information

Taenia solium IgG ELISA Kit

Taenia solium IgG ELISA Kit Taenia solium IgG ELISA Kit Catalog Number KA3191 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of

More information

Barley yellow dwarf virus Reagent Set Compound ELISA, Alkaline phosphatase label Catalog number: SRA (BYDV-MAV), SRA (BYDV-PAV)

Barley yellow dwarf virus Reagent Set Compound ELISA, Alkaline phosphatase label Catalog number: SRA (BYDV-MAV), SRA (BYDV-PAV) List of contents Lot number Barley yellow dwarf virus Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Detection Antibody, bottle A 0.150 ml

More information

Chymotrypsin ELISA Kit

Chymotrypsin ELISA Kit Chymotrypsin ELISA Kit Cat. No.:DEIA10041 Pkg.Size:96T Intended use The Chymotrypsin ELISA Kit is a sandwich Enzyme Immuno Assay intended for the quantitative determination of Chymotrypsin in stool. General

More information

Insulin ELISA. For the quantitative determination of insulin in serum and plasma

Insulin ELISA. For the quantitative determination of insulin in serum and plasma Insulin ELISA For the quantitative determination of insulin in serum and plasma For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United

More information

Draft Agreed by Immunologicals Working Party January Adoption by CVMP for release for consultation 12 March 2009

Draft Agreed by Immunologicals Working Party January Adoption by CVMP for release for consultation 12 March 2009 15 March 2010 EMA/CVMP/IWP/105506/2007 Committee for medicinal products for veterinary use (CVMP) Guideline on data requirements for multi-strain dossiers for inactivated vaccines against avian influenza

More information

HBeAg and HBeAg Ab ELISA Kit

HBeAg and HBeAg Ab ELISA Kit HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3

More information

Insulin ELISA. For the quantitative determination of insulin in serum and plasma.

Insulin ELISA. For the quantitative determination of insulin in serum and plasma. Insulin ELISA For the quantitative determination of insulin in serum and plasma. For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United

More information

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit

Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum

More information

GLP-2 ELISA. For the quantitative determination of GLP-2 in human serum and plasma samples.

GLP-2 ELISA. For the quantitative determination of GLP-2 in human serum and plasma samples. GLP-2 ELISA For the quantitative determination of GLP-2 in human serum and plasma samples. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 48-GP2HU-E01.1 Size: 96 wells Version:

More information

See external label 2 C-8 C 96 tests CHEMILUMINESCENCE. CMV IgG. Cat # Step (20-25 C Room temp.) Volume

See external label 2 C-8 C 96 tests CHEMILUMINESCENCE. CMV IgG. Cat # Step (20-25 C Room temp.) Volume DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol (Cat. No.:EK-310-91) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED USE The

More information

HIV-1 p24 ELISA Kit. purified polyclonal antibody raised against the full length recombinant p24 is used.

HIV-1 p24 ELISA Kit. purified polyclonal antibody raised against the full length recombinant p24 is used. HIV-1 p24 ELISA Kit 80-001 1 kit 96 assays This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwich ELISA (Enzyme Linked Immunosorbent Assay) method. p24 antigen

More information