Fresh Citrus Disinfectant Cleaner

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1 SUMMARY OF ANTIMICROBIAL ACTIVITY Fresh Citrus Disinfectant Cleaner Description Fresh Citrus Disinfectant Cleaner is an effective economical 4 ounce per gallon disinfectant, specifically designed for hospitals, nursing homes, schools, food processing plants, food service establishments and other institutions where housekeeping is of prime importance. It is a phosphate free formulation using a third generation quat and designed to provide effective cleaning, deodorizing and disinfection, controlling the hazard of cross contamination on treated surfaces. Regulatory Summary Physical Properties EPA Registration No ph of Concentrate Flash Point (PMCC) >200 F USDA Authorization None Specific % Quat (mol. wt.360.5) 1.60% min. 25 C California Status Not Pounds per 8.41 registered 25 C % Volatile 90+ Canadian PCP# None Canadian Din # None

2 (rev. 04/2009 jcg) Summary of Antimicrobial Test Results Disinfectant 10 minutes 5% 400 ppm as CaCO 3 Test Method: Official Method of the AOAC, 14 Edition Use Dilution Method Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Pseudomonas N/A 60, 60, 60 1/60, 1/60, 1/60 aeruginosa (4.0 oz/gal) Staphylococcus 6538 N/A 60, 60, 60 0/60, 0/60, 1/60 aureus Salmonella enterica N/A 60, 60, 60 1/60, 0/60, 0/60 Cornynebacterium ppm N/A 10, 10 0/10, 0/10 ammoniagenes Enterococcus N/A 10, 10 0/10, 0/10 Faecalis Enterococcus N/A 10, 10 0/10, 0/10 faecalis (Vancomycin Resistant) (VRE) Escherichia coli N/A 10, 10 0/10, 0/10 Klebsiella ppm N/A 10, 10 0/10, 0/10 pneumoniae Legionella ppm N/A 10, 10 0/10, 0/10 pneumophilia Proteus mirabilis 9240 N/A 10, 10 0/10, 0/10 Salmonella schottmuelleri 8759 N/A 10, 10 0/10, 0/10 Serratia marcescens N/A 10, 10 0/10, 0/10 Shigella N/A 10, 10 0/10, 0/10 dysenteriae Staphylococcus N/A 10, 10 0/10, 0/10 aureus 1 (MRSA) Community N/A 10, 10 0/10, 0/10 Associated Staphylococcus aureus (CA-MRSA) Staphylococcus CDC No. HIP- N/A 10, 10 0/10, 0/10 aureus 2 (VISA) 5836 Streptococcus N/A 10, 10 0/10, 0/10 Faecalis Streptococcus salivarius 7073 N/A 10, 10 0/10, 0/10 Conclusion: All lots of FRESH CITRUS DISINFECTANT CLEANER effectively killed the above listed bacteria as specified in the test performance standards. 1 Resistant to Gentamicin and Methicillin 2 Reduced Susceptibility to Vancomycin

3 Summary of Antimicrobial Test Results - (Continued) Mildewstat 10 minutes 5% N/A Test Method: Mildewstat (Mold and Mildew Control) - EPA - TSD Mildewstat on Hard Surfaces Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Aspergillus niger 6275 (4.0 oz/gal) N/A 10 No growth after 7 days Conclusion: All lots of FRESH CITRUS DISINFECTANT CLEANER were effective against Aspergillus niger under the test conditions outlined in the EPA test performance standards described above. FRESH CITRUS DISINFECTANT CLEANER is an effective mildewstat for non-porous inanimate hard surfaces when diluted to active concentration (4.0 oz. per gallon of water) Fungicide 10 minutes N/A N/A Test Method: AOAC Fungicide method- EPA (DIS/TSS-6) Organism ATCC# Use-Dilution Hard Water Replicates Results Concentration Condition Trichophyton mentagrophytes 9533 (4.0 oz/gal) N/A 10, 10 0/10, 0/10 Conclusion: All lots of FRESH CITRUS DISINFECTANT CLEANER were effective against Aspergillus niger under the test conditions outlined in the EPA test performance standards described above. FRESH CITRUS DISINFECTANT CLEANER is an effective mildewstat for non-porous inanimate hard surfaces when diluted to 600 ppm active concentration (4.0 oz. per gallon of water).

4 Summary of Antimicrobial Test Results - (Continued) Virucide Varies 5% N/A Test Method: EPA Guidelines DIS/TSS-7 Organism Adenovirus Type 2 Source of Virus or ATCC# Host System; Cytopathic Effect Use-Dilution Concentration Contact Time Replicates Reduction (Log 10) of Virus Titer 10 Min. 2 >5.0 Log 10 Herpes Simplex Type 1 Herpes Simplex Type 2 Human Immunodeficiency Virus (HIV 1) Influenza A2/Japan Human Coronavirus Avian Influenza A (H5N1) virus Canine Distemper virus Avian influenza/turkey/ Wisconsin Hepatitis B Virus (HBV) Hepatitis C Virus (HCV) Vaccinia virus Representative of Pox virus group Newcastle Disease Equine Arteritis virus Infectious Avian Laryngotracheitis Porcine Respiratory & Reproductive virus (PRRSV) Infectious Bovine Rhinotracheitis Pseudorabies Virus HSV-1; ATCC VR-733 HSV-2; ATCC VR-734 HTLV-IIIB ATCC VR-740 ATCC VR-798 Hepadnavirus Testing ATCC CCL-22 ATCC VR-119 ATCC VR-188 ATCC VR-135 VERO cells; lytic cytopathic effect VERO cells; lytic cytopathic effect MT2 cells; lytic cytopathic effect 10 Min. 2 >3.25 Log Min. 2 >4.25 Log Min. 2 >4.25 Log Min. 2 >5.0 Log min. 2 >4.25 Log Min. 2 >5.0 Log min. 2 >4.25 Log min. 2 >5.7 Log min. 2 >4.79 Log min. 2 >5.56 Log Min. 2 >4.0 Log Min 2 >3.7 Log Min. 2 >3.0 Log 10

5 Transmissible Gastroenteritis (TGE) ATCC VR min. 2 >3.2 Log 10 Conclusion: All lots of FRESH CITRUS DISINFECTANT CLEANER effectively inactivated the above listed viruses as specified in the test performance standards.

6 Summary of Antimicrobial Efficacy - Etiology 3 Pathogenic Microorganism Staphylococcus aureus Salmonella choleraesuis Aspergillus niger Enterobacter aerogenes Escherichia coli Listeria monocytogenes Klebsiella pneumoniae Proteus mirabilis Salmonella schottmuelleri Serratia marcescens Shigella dysenteriae Hepatitis B virus (HBV) Herpes Simplex Type 1&2 HIV-1 (AIDS Virus) Influenza A virus Vaccinia Description Gram positive bacteria identified as a major cause of hospital acquired (nosocomial) infections. Colonizes food and secretes enterotoxins which cause food poisoning after ingestion. Causes wound infections, septicemia, endocarditis, meningitis, osteomylitis and pneumonia. Required for Hospital Disinfectants. Gram negative bacteria associated with acute gastroenteritis and septicemia. Required for Hospital Disinfectants. Black mold, found in shower and dressing rooms. Environmental contaminant may also cause Aspergillosis. Gram negative bacteria spread by anal/oral route of infection. Associated with bacteremia, respiratory, wound and urinary tract infections. Gram negative bacteria spread by anal/oral route of infection, resulting in diarrhea outbreaks. Associated with urinary tract infections and bacteremia. Gram positive (rod shape) bacteria. Considered a potent food pathogen. Found in raw meat and poultry. Infections can result in meningitis or sepsis. Gram negative bacteria associated with severe pneumonia, bacteremia and urinary tract infections. Gram negative (rod shape) bacteria. Highly motile bacteria. Opportunistic pathogen causes bacteremia, urinary tract infections, especially with the chronically ill. Gram negative (rod shape) bacteria associated with acute gastroenteritis and diarrhea. Gram negative bacteria associated with urinary tract infections, meningitis and septicemia. Gram negative bacteria directly spread by anal/oral route of infection; indirectly (including food, hands, flies) spread by contaminated food and inanimate objects resulting in bacillary dysentery. A hepadnavirus, a relatively new class of viruses. Partially double stranded DNA virus with three distinct morphologies. A blood borne pathogen causes serious degeneration of the liver. Lipophilic (enveloped) DNA virus may result in oral mucocutaneous lesions. Associated with most orofacial herpes and HSV encephalitis. Lipophilic (enveloped) RNA retrovirus. Human Immunodeficiency Virus. Known to be the etiologic agent of Acquired Immunodeficiency Syndrome (AIDS). Lipophilic (enveloped) RNA virus. Causative agent in viral flu. Causes flu epidemics in nearly 2 of every 3 years. Lipophilic (enveloped) DNA poxvirus; causes poxvirus infections. 3 Microbiology, D. Kingsbury and G. Wagner Harwal Publishing 1990

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