Overview on YMC Column Hardware and Phases
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1 Overview on and Phases Contents UHPLC Column Hardware PT and CC...page 02 Analytical Column Hardware PTH and WT...page 03 Analytical Column Hardware QT and QC...page 04 Analytical Column Hardware GC...page 05 Metal-free PEEK-lined Hardware - PTP...page 07 PEEK Hardware WP...page 08 Capillary Column Hardware AU/RU...page 09 (Semi-)Preparative Hardware WX and DX/AX...page 10 Semi Preparative Hardware WT and CC...page 11 Semi Preparative Hardware WTG...page 12 SFC Hardware WTS...page 14 Page 1/15
2 UHPLC Column Hardware (-xxxxpt) 20, 30, 50, 75, 100, 150 mm 2.0, 2.1, 3.0, (4.6) mm Max. pressure: 1,000 bar (YMC-Triart 1.9 µm), 600 bar (Meteoric Core) Port depth: 2.0 mm (0.08 ) Fig. 1: YMC-Triart UHPLC column Fig. 2: Meteoric Core UHPLC/HPLC column UHPLC Guard Cartridges (-xxxxcc) Guard Holder needed: XPCHUHP Fig. 3: UHPLC guard column holder 5 mm 2.1, 3.0 mm Max. Pressure: 1,000 bar Port depth: adjustable Fig. 4: UHPLC guard cartridge Applicable Phase-Hardware Combinations Family Modification Particle Size PT WT (UltraHT) CC (Guard) C µm X X C18 ExRS 1.9 µm X X YMC-Triart C8 1.9 µm X X Phenyl 1.9 µm X X PFP 1.9 µm X X Diol-HILIC 1.9 µm X X YMC-Pack Pro C18 2 µm X X Hydrosphere C18 2 µm X X C µm X Meteoric Core C18 BIO 2.7 µm X C8 2.7 µm X Page 2/15
3 Higher Pressure HPLC Column Hardware (-xxxxpth) 20, 33, 50, 75, 100, 150, 250 mm 2.1, 3.0, 4.6 mm Max. Pressure: 450 bar Port depth: 2.0 mm (0.08 ) Fig. 5: YMC-Triart high-pressure HPLC column HPLC Column Hardware (-xxxxwt) 30, 50, 75, 100, 150, 250 mm 1.0, 2.0, 3.0, 4.0, 4.6 mm Max. Pressure: 300 bar (3 µm, 150/250 mm length; CHIRAL ART) 250 bar (250 mm length) 200 bar ( 150 mm length) 100 bar (10-30 mm ID) 500 bar (2 µm, UltraHT) Port depth: 3.3 mm (0.13 ) Fig. 6: YMC HPLC column - Waters type Port depth Fig. 7: YMC-Triart HPLC column Fig. 8: YMC-UltraHT HPLC column Page 3/15
4 HPLC Cartridge Type Hardware (-xxxxqt) 30, 33, 50, 100, 125, 150, 250 mm 2.1, 3.0, 4.0, (4.6) mm Max. Pressure: 250 bar (250 mm length) 200 bar ( 150 mm length) Port depth: 2.3 mm (0.09 ) Fig. 9: HPLC cartridge type column (2.1 mm ID) Fig. 10: HPLC cartridge type column (3/4 mm ID) HPLC Cartridge Hardware (-xxxxqc) 30, 33, 50, 100, 125, 150, 250 mm 2.1, 3.0, 4.0, (4.6) mm Max. Pressure: 250 bar (250 mm length) 200 bar ( 150 mm length) Port depth: 2.3 mm (0.09 ) Fig. 11: HPLC cartridge (2.1 mm ID) Fig. 12: HPLC cartridge (3/4 mm ID) Page 4/15
5 Analytical Guard Cartridges (-xxxxgc) Guard Holder needed: External/universal holder, for use with PTH, WT, QT hardware. Internal holder, for use with QT hardware only. For 10 mm length XPGCH-Q1 For 10 mm length XPGCS-Q1 For 20 mm length XPGCH-Q2 For 20 mm length XPGCS-Q2 Fig. 13: universal HPLC guard cartridge holder Fig. 14: internal HPLC guard cartridge holder 10, 20 mm mm Max. Pressure: 400 bar (6.000 psi) Port depth: adjustable Fig. 15: HPLC guard cartridge Applicable Phase-Hardware Combinations Family Modification Particle Size PTH WT QT QC GC C18 3, 5 µm X X X C18 ExRS 3, 5 µm X X YMC-Triart YMC-Pack Pro YMC-Pack C8 3, 5 µm X X X Phenyl 3, 5 µm X X X PFP 3, 5 µm X X X Diol-HILIC 3, 5 µm X X X C18 3, 5 µm X X X X Hydrosphere C18 3, 5 µm X X X X C18 RS 3, 5 µm X X X X C8 3, 5 µm X X X X C4 3, 5 µm X X X X Diol SEC 3, 5 µm X X X X Diol SEC UHPLC 2 µm X Page 5/15
6 Applicable Phase-Hardware Combinations Family Modification Particle Size WT QT QC GC ODS-A 3, 5 µm X X X X ODS-AQ 3, 5 µm X X X X ODS-AL 3, 5 µm X X X X ODS-AM 3, 5 µm X X X X Polymer C18 6 µm X X X X C 8 (Octyl) 3, 5 µm X X X X C 4 (Butyl) 3, 5 µm X X X X YMC-Pack Ph (Phenyl) 3, 5 µm X X X X Protein-RP 5 µm X X X X TMS (C1) 3, 5 µm X X X X CN (Cyano) 3, 5 µm X X X X PVA-SIL 5 µm X X X X Polyamine II 5 µm X X X X NH 2 (Amino) 3, 5 µm X X X X Diol-NP 3, 5 µm X X X X SIL (Silica) 3, 5 µm X X X X YMC Carotenoid 3, 5 µm X X X X Specialities YMC-PAH 3, 5 µm X X X X J Sphere ODS 4 µm X X X X YMCbasic 3, 5 µm X X X X Amylose-C 3, 5 µm X X Cellulose-C 3, 5 µm X X CHIRAL ART Amylose-SA 3, 5 µm X X Cellulose-SB 3, 5 µm X X Cellulose-SC 3, 5 µm X X YMC-CHIRAL NEA (R)(S) 5 µm X X CD BR 5 µm X X Page 6/15
7 Metal-free PEEK-lined Hardware (-xxxxptp) 50, 100, 150 mm 2.1, 4.6 mm Max. Pressure: 450 bar (3/5 µm) 1000 bar (1.9 µm) Port depth: 2.0 mm (0.08 ) Fig. 16: YMC-Triart metal-free column Applicable to following products only Family Modification Particle Size C18 YMC-Triart C18 ExRS C8 Phenyl PFP 1.9, 3, 5 µm Diol-HILIC Page 7/15
8 Biocompatible PEEK Hardware (-xxxxwp) Max. Pressure: Port depth: 30, 50, 100 mm 4.6 mm 30 bar (porous 5 µm) bar (non-porous 5 µm) 250 bar (non-porous 3 µm) 3.3 mm (0.13, Waters style) Fig. 17: YMC-BioPro column Applicable to following products only Family Modification Particle Size SP 5 µm YMC-BioPro SP-F 3, 5 µm QA 5 µm QA-F 3, 5 µm Page 8/15
9 Capillary Column Hardware (-xxxxau/-xxxxru) 50, 75, 100, 150, (250) mm 75, 100, 300, 500 µm Max. Pressure: 550 bar for 2, 3,5 µm 600 bar for 1.9 µm Fitting: 1/16 (AU) 1/32 (RU) Thread: 6-40 (1/32 ) (1/16 ) Fig. 18: YMC capillary columns Fig. 19: YMC capillary guard columns Applicable to following products All YMC RP, NP, HILIC phases. Page 9/15
10 (Semi-)Preparative Hardware YMC-Actus Semi Prep. Hardware (20/30 mm ID) (-xxxxwx) 50, 75, 100, 150, 250 mm 20, 30 mm Max. Pressure: 300 bar Port depth: 3.3 mm (0.13 ), 1/16 Fig. 20: YMC-Actus semi prep. column Waters type Port depth YMC-Actus Prep. Hardware (50 mm ID) (-xxxxdx/-xxxxax) 100, 150, 250 mm 50 mm Max. Pressure: 200 bar Connection: female Swagelok nut connection (DX, 1/8 ) Port depth: 3.3 mm (0.13, AX, 1/16 ) Fitting: 1/8 (DX) 1/16 (AX) Fig. 21: YMC-Actus 50 mm, 1/8 prep. column Waters type Port depth Page 10/15
11 Semi Prep. Hardware (10/20/30 mm ID) (-xxxxwt) 100, 150, 250, 300 mm 10, 20, 30 mm Max. Pressure: 100 bar Port depth: 3.3 mm (0.13 ), 1/16 Fig. 22: standard semi-prep. column Waters type Port depth Semi Prep. Guard Cartridges (-xxxxcc) Guard Holder needed: For use with WT, WX hardware. For 10 mm ID XPCHSPW1 For 20 mm ID XPCHSPW2 For 30 mm ID XPCHSPW3 Fig. 23: semi prep. guard cartridge holder Fig. 24: semi prep. guard cartridge 10 mm 10, 20, 30 mm Max. Pressure: 300 bar Port depth: 3.3 mm (0.13 ), 1/16 Page 11/15
12 Semi Prep. Guard Column Hardware (-xxxxwtg) 30 mm 8, 10, 20, 30 mm Max. Pressure: 100 bar Port depth: 3.3 mm (0.13 ), 1/16 Fig. 25: semi prep. guard column Waters type Port depth Applicable Phase-Hardware Combinations Family Modification Particle Size WX WT DX/AX CC WTG C18 5 µm X X* X X X C18 ExRS 5 µm X X* X X X YMC-Triart YMC-Pack Pro C8 5 µm X X* X X X Phenyl 5 µm X X* X X X PFP 5 µm X X* X X X Prep C18-S 10, 15, 20 µm X X* X X X Prep C8-S 10, 15, 20 µm X X* X X X C18 5 µm X X* X X X Hydrosphere C18 5 µm X X* X X X C18 RS 5 µm X X* X X X C8 5 µm X X* X X X C4 5 µm X X* X X X *only for 10 mm ID available, for 20/30 mm YMC-Actus hardware available Page 12/15
13 Applicable Phase-Hardware Combinations Family Modification Particle Size WX WT DX/AX CC WTG ODS-A 5 µm X X* X X X ODS-AQ 5 µm X X* X X X ODS-AL 5 µm X X X X X ODS-AM 5 µm X X X X X C 8 (Octyl) 5 µm X X X C 4 (Butyl) 5 µm X X X Ph (Phenyl) 5 µm X X X YMC-Pack Protein-RP 5 µm X X X TMS (C1) 5 µm X X X CN (Cyano) 5 µm X X X PVA-SIL 5 µm X X X Polyamine II 5 µm X X X NH 2 (Amino) 5 µm X X X Diol 5 µm X X X Diol-NP 5 µm X X X SIL (Silica) 5 µm X X X Specialities YMCbasic 5 µm X X X X X YMC Omega 10 µm X X* X X Amylose-C 5 µm X X* X X X Cellulose-C 5 µm X X* X X X CHIRAL ART Amylose-SA 5 µm X X* X X X Cellulose-SB 5 µm X X* X X X Cellulose-SC 5 µm X X* X X X YMC-CHIRAL NEA (R)(S) 5 µm X X X CD BR 5 µm X X X *only for 10 mm ID available, for 20/30 mm YMC-Actus hardware available Page 13/15
14 SFC Hardware Alycon SFC-Hardware (-xxxxwts) Length: 50, 100 mm for 3 µm 150, 250 mm for 3, 5 µm 2.1, 3.0 mm for 3 µm 2.1, 4.6 mm for 3, 5 µm 10, 20 mm for 5 µm Max. Pressure: 300 bar (2.1/4.6 mm ID) 200 bar (10/20 mm ID) Port depth: 3.3 mm (0.13 ) Fig. 26: Alycon SFC columns Waters type Port depth Applicable to following products only Family Modification Particle Size C18 5 µm PFP 5 µm Alcyon SFC Diol-HILIC 5 µm CN 5 µm SIL 5 µm Amylose-C 3, 5 µm Cellulose-C 3, 5 µm Alcyon SFC CSP Amylose-SA 3, 5 µm Cellulose-SB 3, 5 µm Cellulose-SC 3, 5 µm Page 14/15
15 Schöttmannshof Dinslaken Phone: +49 (0) Fax: +49 (0) Page 15/15
Overview on YMC Column Hardware and Phases
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SIMPLIFIED CHIRAL SEPARATIONS. FIVE Complementary Chiral Phases. for Greater Selectivity. Resolve 92 %
SIMPLIFIED IRAL SEPARATIS FIVE omplementary hiral Phases for Greater Selectivity Resolve 9 % of all your Enantiomers using Screening Kits * FREE hiral Screening and Separation Services. *Based on screening
Transfer. Flexible. Universal. 12 YMC-Triart. Scalable particles: EASY UHPLC HPLC. YMC-Triart: ph 1-12 Temperatures up to 70 C
2 YMC-Triart Transfer Scalable particles: EASY UHPLC HPLC Flexible YMC-Triart: ph -2 Temperatures up to 70 C Universal YMC-Triart for acidic, basic and neutral analytes YMC-Triart 3 YMC-Triart Contents
APPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology.
Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology Jeff Layne, Phenomenex, Inc. Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 951 USA With virtually
LC Columns - Exceed the limit. A premium inert range of LC columns delivering optimal peak shape. ProteCol -P PEEK lined
ProteCol LC Columns - Exceed the limit A premium inert range of LC columns delivering optimal peak shape. ProteCol -G Glass lined ProteCol -P PEEK lined B C A D E F A Column end cap B PEEK frit housing
For Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns.
For Far Greater UHPLC Column Performance... You Must Have Kinetex Core-Shell UHPLC Columns www.phenomenex.com/kinetex The UHPLC Core-Shell Advantage Kinetex Core-Shell Technology produces increased efficiencies
Andrea Perissi Specialista Applicativo, Waters Italia Waters Corporation 1
Interfacing UPC 2 to MS Andrea Perissi Specialista Applicativo, Waters Italia 2012 Waters Corporation 1 Agenda Introduction Traditional approaches to interfacing SFC to MS Ionization Mode Approach used
Unequalled durability against water elution. % tr
Unequalled durability against water elution 2 Revolutionary aqueous durability for aminopropyl phase 1 3 4 1. fructose (2.5mg/mL) 3. sucrose (2.5mg/mL) 500 hr 2. glucose 400 hr Aqueous to non-aqueous Normal
HIGH PERFORMANCE SEPARATION PHASES
HIGH PERFORMANCE SEPARATION PHASES HPLC UHPLC LC/MS Flash routine analysis polar substance separation special ion analysis ultra-fast analysis 100% water compatibility biomacromolecule analysis LC/MS compatibility
UHPLC / HPLC Columns Developed for Mass Spectrometry
ACE UltraCore SuperC18 UHPLC / HPLC s Developed for Mass Spectrometry Water analysis Pharmaceutical Environmental Toxicology Food safety Clinical Forensics Metabolism Trace analysis Ultra-inert 2.5µm and
Pharmacopoeia Methods
Allowable Adjustments to Pharmacopoeia Methods Isocratic Separations Component European Pharmacopoeia (Ph. Eur.) Composition of the mobile phase ± 30 % Relative or ± 2 % absolute, whichever is the larger;
Phenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases
Phenyl-Hexyl UHPLC Columns lternate, complementary selectivity to A C8 and C8 bonded phases articularly recommended for P compounds containing aromatic groups xcellent bonded phase stability for E durable,
Columns The FIRST and ONLY sub-2 µm core-shell columns on the market
Better UHPLC Performance Starts NW.7 µm Core-Shell * Columns The IRST and NLY sub- µm core-shell columns on the market Phenomenex India Laxmi Cyber City, Ground loor, B Block, Survey no: Kondapur, Hyderabad
HPLC Columns. HPLC Accessories HPLC Column Test Mixes Reference Books...www.restek.com. HPLC Applications...
HPLC Columns HPLC Column Selection................................292-297 UHPLC Columns........................................298-299 Capillary LC Columns........................................300 Pinnacle
HT5 - High Temperature Stationary Phase for Capillary Gas Chromatography
HT5 - High Temperature Stationary Phase for Capillary Gas Chromatography INTRODUCTION HT5 was the first carborane modified siloxane phase to be commercially available on fused silica capillary columns.
(U)HPLC columns for polar analytes
(U)HPLC columns for polar analytes The Selectivity Company YMC-Triart C8 for 00% aqueous conditions Universal 2 columns in : C8 phase with AQ compatibility Flexible UHPLC HPLC ph = - 2 Temp. up to 70 C
InertSustainBio C18. Rapid Separations of Proteins and Peptides
InertSustainBio C18 Rapid Separations of Proteins and Peptides High Recoveries of Proteins and Peptides The 200A pore size silica creates the opportunity to separate compounds having a molecular weight
SPME-LC Fibers for a Variety of Applications
SPME-LC Fibers for a Variety of Applications Robert Shirey 1, Craig Aurand 1, Dajana Vuckovic 2, Katherine Stenerson 1, Yong Chen 1, Leonard Sidisky 1, and Janusz Pawliszyn 2 1 Supelco, Div. of Sigma-Aldrich,
InertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior
InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups,
Purification - Flash Chromatography
SuperFlash Compound Purification Columns Optimal Design Cost effective, high performance disposable flash column specifically designed for flash chromatography. Excellent Separations Designed to deliver
ACE. Intelligent Solutions for Method Development. Method Development Kits. ACE UHPLC and HPLC Columns. a a
r: ff e ts ice O ki r ti y mn e p tiv olu sam mn c c u le the col e d S an for ngle le si ab s a l i a a av ACE Method Development Kits Intelligent Solutions for Method Development Highly cost effective
Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC
Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC Bàrbara Bagó Bilbao, 12 Julio 2012 2011 Waters Corporation 1 Agenda Column Manufacturing and Platform Definitions New HPLC & ACQUITY UPLC Column Chemistries
314 Speciality Columns
314 Speciality Columns Speciality Columns 315 Speciality Columns Contents YMC Carotenoid... 316-317 YMC PAH... 318-319 J sphere ODS... 320-325 Ordering Information... 326 Introduction Unique bonded phases
Raptor HILIC-Si: Simplify the Switch to HILIC
Stationary Phase: Silica Selectivity Accelerated Raptor HILIC-Si: Simplify the Switch to HILIC Retain polar compounds without ion-pairing reagents. 2.7 µm Raptor core-shell particles provide the speed
Versatile UHPLC columns
Versatile UHPLC columns The Selectivity Company Transfer EASY UHPLC HPLC 100 MPa / 1000 bar Flexible ph = 1 12 Temperature up to 70 C Universal for acidic, basic and neutral analytes www.ymc.de 2 Specification...
COMBO ULTIMATE HPLC/UHPLC. Kinetex + Luna Omega NEW. Gain Incredible Performance, Versatile Selectivities and Upgrade Throughput
ULTIMATE HPLC/UHPLC CMB Kinetex Luna mega Gain Incredible Performance, Versatile Selectivities and Upgrade Throughput NEW MEGA Core-Shell Technology Luna mega Luna mega PS Kinetex. Kinetex Luna mega Cutting
Raptor HILIC-Si: Simplify the Switch to HILIC
Stationary Phase: Silica Selectivity Accelerated Raptor HILIC-Si: Simplify the Switch to HILIC Retain polar compounds without ion-pairing reagents. 2.7 µm Raptor core-shell particles provide the speed