RNA and Protein Synthesis Guided Notes
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1 RNA and Protein Synthesis Guided Notes is responsible for controlling the production of in the cell, which is essential to life! o DNARNAProteins contain several thousand, each with directions to make one Where are Proteins Produced? Proteins are produced on! Found in two places: o Free floating in o Attached to How does information needed to build a protein gets delivered from the DNA to the ribosomes? o With the help of in a process called What is RNA? RNA stands for One subunit is called a o 1 5-carbon (ribose) o 1 group o 1 nitrogenous Three types of RNA: A Closer Look at mrna How is mrna different from DNA? o stranded o and able to leave the o The sugar is o There is a different base (U) takes the place of (T) The job of mrna is to take directions for one gene and transport it to a in the. o This is so a cell can begin assembling, the building blocks of! o It s sending a on how to do the job!
2 Protein Synthesis Protein synthesis is a two stage process: o A molecule (mrna) carries instructions from DNA to ribosomes DNA leave the nucleus; can! makes it possible for to be assembled by outside the nucleus Protein Synthesis: Transcription Transcription is when is turned to Happens when need to be made in the Since DNA CANNOT leave the, it is into RNA (DNARNA) o Transcribe: (copy in the same nucleic acid language, but only what is needed!) How does it happen? 1) After an targets the portion of the DNA that should be copied ( ), the sections of DNA ( ) will temporarily to allow mrna to (copy). This will continue until an enzyme signals the end 2) mrna leaves the, travels into the and attaches to a 3) The message from DNA can now be translated to make a Think about it: What is happening in each photo below during transcription? Transcribing DNA to mrna is very easy if you remember these complementary pairs! Try it! o A piece of DNA reads: T A G C A T T C C G A U Transcribe to mrna: o 1 side of DNA reads: A A G C G T A T C C C G Transcribe to mrna:
3 Protein Synthesis: Translation Translation The process in which is used as a to form chains of (RNAProtein) o Amino acids linked together form a o Translate: To change a sentence from one language ( ) to another ( ) Every 3 letters on an mrna chain = Each codon (3 DNA letters) = 1 Given the, we can read a chart to translate it into amino the amino acid it codes for! o Remember, 1 word in nucleic acid language is a (three nucleotides) Think about it: What amino acid is coded for? 1) AUG 2) GUC 3) GCC 4) CGA 5) UAA Translation occurs in a in ALL cells Uses all three forms of RNA ( ) DNA is not directly used! trna: A Closer Look Steps of Translation 1) The mrna leaves the and lands on a (rrna) 2) (with correct anticodon) lands on the ribosome opposite a on the mrna 3) The trna leaves the ribosome, but the that it coded for stays on the ribosome to wait for next codon to be read 4) The moves to the next bringing in another to the growing protein chain Notice the trna is carrying the amino acid leucine, coded for by the sequence CUA The trna knows how to match using bases! So mrna codon reads CUA, so the trna anticodon will be GAU
4 The amino acid chain will ALWAYS begin with the - AUG The trna will continue to add amino acids until it reaches a (UAA, UAG, UGA) When it reaches a stop codon, then a complete has been built! The protein from the ribosome. Think about it: Label the diagram of translation to the right with the following terms! a) ribosome b) mrna c) trna d) codon e) anticodon f) amino acid chain Let s Practice! Given the strand of DNA ATC o What would it s complementary DNA strand read? o Now, transcribe the DNA to mrna o What amino acid does the codon code for? (use chart) o What would the anticodon on trna read? Given the strand of DNA TGA o What would it s complementary DNA strand read? o Now, transcribe the DNA to mrna o What amino acid does the codon code for? (use chart) o What would the anticodon on trna read? Mutations Changes to DNA are called o Change the o Change the o May change o May change
5 Types of Mutations Changes to the letters (ATGC bases) in DNA! Point mutation change to letter in the DNA! o May (or may not) cause a change to protein Frame shift mutation addition of a letter; or deletion of a letter! o Both of these DNA so it changes how the codons are read o Big changes to protein Point Mutations Missense mutation = (Ex. Sickle Cell Anemia) Silent mutation= Nonsense mutation= Frameshift Mutations or one or more bases o Change the meaning of the whole protein! Addition Deletion
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