UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
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2 EDITORIAL BOARD EDITORIAL BOARD OF DENTAL JOURNAL (MAJALAH KEDOKTERAN GIGI), January 2nd, 2014 December 31st, 2014 SK: 166/H3.1.2/KD/2014 January 2 nd, 2014 December 31 st, 2014 Patron : Dean of Faculty of Dentistry Airlangga University Advisors : Vice Dean I, Vice Dean II, Vice Dean III Chief Editor : Udijanto Tedjosasongko, drg., Ph.D.,Sp.KGA(K) (Department of Pediatric Dentistry Universitas Airlangga ) Editorial Boards : 1. Prof. Nairn Hutchinson Fulton Wilson, MSc. Ph.D., FDS. (Department of Conservative Dentistry - University of Guy s Dental School, London) 2. Prof. W.J. Spitzer, DMD., MD. (Department of Cranio & Oral Maxillofacial Surgery Hamburg University, Germany) 3. Prof. Edward C. Combe. M.Sc. Ph.D. D.D.Sc. (Department of Biomaterial Minnesota University, U.S.A) 4. Prof. Madya. H. AB. Rani Samsudin D.D.S., FDSRC, AM. (Department of Oral and Maxillofacial Surgery -University Science Malaysia, Malaysia) 5. Prof. Taizo Hamada, D.D.S., Ph.D. (Department of Prosthodontic - Tohoku University, Japan) 6. Prof. Yukio Kato, D.D.S., Ph.D. (Department of Oral Bio Chemistry-University of Hiroshima, Japan) 7. Prof. dr. A.G.M. Tielens (Department of Medical Microbiology and Infections Disease - Erasmus University Medical centre, Rotterdam, the Netherlands) 8. Prof. Lakshman Samaranayake (Department of Oral Microbiology The University of Hongkong) 9. Prof. Kozai Katsuyuki, DDS.,Ph.D. (Department of Pediatric - University of Hiroshima, Japan) 10. Prof. dr. A.G.M. Tielens (Department of Medical Microbiology - University and Infections Diseases - erasmus University Medical Centre, Rotterdam, The Netherlands) 11. Kok Van Kessel (Department of Medical Microbiology and Infections Disease - Erasmus University Medical centre, Rotterdam, the Netherlands) 12. Dr. Leslie Ang (Department of Restorative Dentistry National Dental Centre of Singapore) 13. Prof. Widowati Witjaksono, drg., Ph.D. (Kulliyah of Dentistry, International Islamic University Malaysia) 14. Prof. Dr. M. Suharsini, drg, M.S., Sp. KGA. (Department of Pediatric Dentistry - Universitas Indonesia) 15. Achmad Gunadi, drg, M.S., Ph.D. (Department of Prosthodontic Universitas Jember) Managing Editors : 1. Prof. Dr. M Rubianto, drg, M.S., Sp. Perio. (Department of Periodontic Universitas Airlangga) 2. Prof. Dr. Latief Mooduto, drg, M.S., Sp. KG. (Department of Conservative Dentistry Univ. Airlangga) 3. Prof. RM. Coen Pramono, drg., SU., Sp.BM(K) (Department of Oral and Maxillofacial Surgery - Univ. Airlangga) 4. Prof. Dr. Mieke Sylvia M. A. R., drg, M.S.,Sp.Ort. (Department of Ortodontic Univ. Airlangga) 5. Prof. Dr. Istiati, drg, M.S. (Department of Oral Pathology and Maxillofacial Univ. Airlangga) 6. Prof. Dr. Anita Yuliati, drg, M.Kes. (Department of Dental Material Univ. Airlangga) 7. Prof. Seno Pradopo, drg, S.U., Ph.D. Sp. KGA. (Department of Pediatric Dentistry Univ. Airlangga) 8. Prof. Dr. Diah Savitri Ernawati, drg., M.Si. (Department of Oral Medicine Univ. Airlangga) 9. Prof. Thalca I. Agusni, drg, MHPEd. Ph.D.,Sp.Ort. (Department of Orthodontic Univ. Airlangga) 10. Dr. R. Darmawan Setijanto, drg.,m.kes. (Department of Dental Public Health Univ. Airlangga) 11. Dr. Elly Munadziroh, drg.,ms (Department of Dental Material Universitas Airlangga) 12. Ketut Suardita, drg., Ph.D., Sp.KG(Department of Conservative Dentistry-Univ. Airlangga) 13. Priyawan Rachmadi, drg, Ph.D. (Department of Dental Material Univ. Airlangga) page 2 / 5
3 14. Dr. Retno Pudji Rahayu, drg., M.Kes. (Department of Oral Pathology and Maxollofacial Univ. Airlangga) 15. Dr. Eha Renwi Astuti, drg., M.Kes (Department of Dental Radiology Univ. Airlangga) 16. Bagus Soebadi, drg, MHPEd. (Department of Oral Medicine Univ. Airlangga) 17. Markus Budi Rahardjo, drg.,m.kes. (Department of Oral Biology Univ. Airlangga) 18. Dr. Susy Kristiani, drg., M.Kes. (Department of Odontology Forensic Univ. Airlangga) 19. Dr. Ira Widjiastuti, drg, M.Kes.Sp.KG. (Department of Conservative Dentistry Univ. Airlangga) 20. Sianiwati Goenharto, drg., M.S. (Department of Ortodontic Univ. Airlangga) 21. Devi Rianti, drg, M.Kes. (Department of Dental Material Univ. Airlangga) 22. Dr. Chiquita Prahasanti, drg.,sp.perio. (Department of Periodontic Univ. Airlangga) 23. Rostiny, drg.,m.kes.,sp.pros. (Department of Prosthodontic Univ. Airlangga) 24. An nissa Chusida, drg.,m.kes (Department of Odontology Forensic Univ. Airlangga) 25. Eric Priyo Prasetyo, drg., Sp.KG. (Department of Conservative Dentistry Univ. Airlangga) 26. Els Sunarsih Budipramana, drg., MS.,Sp.KGA.(K). (Department of Pediatric Dentistry Univ. Airlangga) 27. Dr. Hendrik Setiabudi, drg., M.Kes (Department of Oral Biology Univ. Airlangga) 28. Otty Ratna Wahyuni, drg., M.Kes. (Department of Dental Radiology Univ. Airlangga) 29. Anis Irmawati, drg.,m.kes. (Department of Oral biology Univ. Airlangga) 30. Yuliati, drg.,m.kes. (Department of Oral Biology Univ. Airlangga) 31. Retno Palupi, drg.,m.kes. (Department of Dental Public Helath Univ. Airlangga) 32. Eka Augustina, drg.,sp.perio. (Department of Periodonsia Univ. Airlangga) 33. Febriastuti, drg.,sp.kg. (Department of Conservative Dentistry Univ. Airlangga) 34. Mega M. Puteri, drg.,sp.kga. (Department of Pediatric Dentistry Univ. Airlangga) Administrative Assistant : Novi Dian Prastiwi (Faculty of Dentistry Airlangga University) page 3 / 5
4 Table of Contents No Title Page 1 Management of idiopathic alveolar bone necrosis associated with oroantral fistula - after upper left first molar extraction 2 Indirect veneer treatment of anterior maxillary teeth with enamel hypoplasia - 3 Computer aided diagnosis for osteoporosis based on trabecular bone analysis using panoramic radiographs Anti-inflammation effects of Sardinella longicep oils against paw oedema on Rattus novergicus induced by 1% carrageenan The correlation between immunoexpression of estrogen receptor and the severity of periodontal disease The effectiveness of sharp end and rounded end bristle toothbrush Contact hypersensitivity after using tongue piercing Cervical end preparation design of collarless metal ceramic crown to the decrease of bacterium colony number Treatment of sharp mandibular processus alveolaris with hybrid prosthesis The combination of miacalcic, calcium lactate, and vitamin C as post-extraction alveolar bone resorption inhibitor 11 The molecular pheomena of the blaz genes forming beta-lactamase enzymes structure in Staphylococcus aureus resistant to beta-lactam antibiotics (ampicillin) page 4 / 5
5 Powered by TCPDF ( UNIVERSITAS AIRLANGGA Vol No. 3 / TOC : 9, and page : The molecular pheomena of the blaz genes forming beta-lactamase enzymes structure in Staphylococcus aureus resistant to beta-lactam antibiotics (ampicillin) The molecular pheomena of the blaz genes forming beta-lactamase enzymes structure in Staphylococcus aureus resistant to beta-lactam antibiotics (ampicillin) Author : Mieke Satari mieke_satari@yahoo.com Department of Oral Biology Faculty of Dentistry, Padjadjaran University Bandung - Indonesia Abstract Background: Nowadays, infectious diseases still becomes problems. One of the bacteria causing infectious diseases is S. aureus. In the effort to deal with infections caused by S. aureus, beta-lactam antibiotics, such as ampicillin, are used. In fact, it is unfortunately known that many of S. aureus bacteria are resistant to this group of antibiotics. One of the reasons is known because of nucleotide base changes in the structure of the genes (blaz) which encode beta-lactamase enzymes in S. aureus. Purpose: The objective of this study is to analyze the nucleotide base changes in the structure of the genes (blaz) forming beta-lactamase enzymes in S. aureus resistant to ampicillin based on molecular point of view. Methods: Molecular examinations was conducted by isolating the genes, forming beta-lactamase enzyme, which length was 845bp, from 7 isolates of S. aureus resistant to ampicillin by using PCR technique. The results of blaz amplification were then subjected to homology by using Tn 552 of S. aureus obtained from Bank of Genes. Rresults: Based on the result of the homology, it was found that there was a change in purine base T G, which was a pyrimidine base at the -37 position of the initial codon of blaz. This change, however, did not affect the strength of the promoter since the number of A and T is still more than the number of G and C. In the structure of the gene (blaz) there was even no mutation or deletion or nucleotide base substitution found, so it would not affect the effectiveness of beta-lactamase enzyme. Conclusion: It can be concluded that the resistance of S. aureus towards ampicillin is not caused by nucleotide base deletion/substation. Instead, it is suspected that there are other causes leading to the resistance, including the overproduction of beta-lactamase enzyme, causing the degradation of beta-lactam antibiotics conducted by the beta-lactamase enzyme. Keyword : S., aureus, ampicillin, blaz, Daftar Pustaka : 1. Peterson LJ, (1998). Principles of management and prevention of odontogenic infection. St Louis : Mosby Year Book Inc Copy alamat URL di bawah ini untuk download fullpaper : journal.unair.ac.id/filerpdf/dentja2e1eaf92c2full.pdf page 5 / 5
UNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
page 1 / 5 EDITORIAL BOARD EDITORIAL BOARD OF DENTAL JOURNAL (MAJALAH KEDOKTERAN GIGI), January 2nd, 2014 December 31st, 2014 SK: 166/H3.1.2/KD/2014 January 2 nd, 2014 December 31 st, 2014 Patron : Dean
More informationUNIVERSITAS AIRLANGGA DIREKTORAT PENDIDIKAN Tim Pengembangan Jurnal Universitas Airlangga Kampus C Mulyorejo Surabaya
page 1 / 6 EDITORIAL BOARD EDITORIAL BOARD OF DENTAL JOURNAL (MAJALAH KEDOKTERAN GIGI), January 2nd, 2014 December 31st, 2014 SK: 166/H3.1.2/KD/2014 January 2 nd, 2014 December 31 st, 2014 Patron : Dean
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